Differentiation Of Protoplasts And Of Transformed Plant Cells

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Differentiation of Protoplasts and of Transformed Plant Cells

Author : J. Reinert
Publisher : Springer Science & Business Media
ISBN 13 : 3540398368
Total Pages : 163 pages
Book Rating : 4.5/5 (43 download)

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Book Synopsis Differentiation of Protoplasts and of Transformed Plant Cells by : J. Reinert

Download or read book Differentiation of Protoplasts and of Transformed Plant Cells written by J. Reinert and published by Springer Science & Business Media. This book was released on 2013-06-29 with total page 163 pages. Available in PDF, EPUB and Kindle. Book excerpt: H. BINDING and J. REINERT In collaboration with the first authors of this volume This volume is devoted to the development of cell clones and plants from ma nipulated cells: isolated protoplasts, cell fusion bodies, and transformed cells. Isolated protoplasts represent cells which are liberated from their walls and separated from the differentiation pattern of the organism. Investigations on re generation from protoplasts provide a better understanding of the process and control of developmental pathways. Whereas protoplast isolation results in alteration of the state of differentiation of a cell, protoplast fusion is a means for the creation of cells with novel genetic constitution. Fascinating features are (1) to hybridize cells which-unlike gametes -did not derive from meiosis products, (2) to bring together foreign plastids and mitochondria and to investigate their parasexual reactions, and (3) to match ge netic traits which had been separated for long periods of evolution. Highly sophisticated techniques have already been elaborated for the transfer of genes by the use of isolated DNA and gene transfer systems. Highly promising results have already been obtained by the use of Ti plasmids of Agrobacterium, but direct DNA transformation is also proving to be useful. Most of the results in these areas are preliminary and/or limited to a few sys tem.lt is the aim of this volume to present the main features, but at the same time to draw attention to problems and perspectives of protoplast regeneration and so matic cell genetics in order to stimulate further investigations.

PLANT PROTOPLASTS

Author : Fowke
Publisher : Boca Raton, Fla. : CRC Press
ISBN 13 :
Total Pages : 264 pages
Book Rating : 4.3/5 (91 download)

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Book Synopsis PLANT PROTOPLASTS by : Fowke

Download or read book PLANT PROTOPLASTS written by Fowke and published by Boca Raton, Fla. : CRC Press. This book was released on 1985-10-04 with total page 264 pages. Available in PDF, EPUB and Kindle. Book excerpt: Protoplast isolation and culture; Regeneration of plants; Protoplasts for studies of cell organelles; Protoplast fusion, Plant protoplasts and the cell cycle; Isolated protoplasts as laboratory tools in the study of cell wall deposition; Cytodifferentiation; The use of protoplasts for studies on membrane transport in plants; Protoplasts in virology; Plant protoplasts and genetic variation; Plant mutant isolation via protoplasts; Hybridization by somatic cell fusion; Plant protoplast transformation by Agrobacterium in relation to plant biotechnology.

Plant Protoplasts and Genetic Engineering VII

Author : Y. P. S. Bajaj
Publisher : Springer Science & Business Media
ISBN 13 : 3662093685
Total Pages : 339 pages
Book Rating : 4.6/5 (62 download)

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Book Synopsis Plant Protoplasts and Genetic Engineering VII by : Y. P. S. Bajaj

Download or read book Plant Protoplasts and Genetic Engineering VII written by Y. P. S. Bajaj and published by Springer Science & Business Media. This book was released on 2013-03-14 with total page 339 pages. Available in PDF, EPUB and Kindle. Book excerpt: Twenty-seven chapters deal with the regeneration of plants from protoplasts and genetic transformation in various species of Agrostis, Allium, Anthriscus, Asparagus, Avena, Boehmeria, Carthamus, Coffea, Funaria, Geranium, Ginkgo, Gladiolus, Helianthus, Hordeum, Lilium, Lithospermum, Mentha, Panax, Papaver, Passiflora, Petunia, Physocomitrella, Pinus, Poa, Populus, Rubus, Saintpaulia, and Swertia. These studies reflect the far-reaching implications of protoplast technology in genetic engineering of plants. This volume is of special interest to advanced students, teachers, and research scientists in the field of plant tissue culture, molecular biology, genetic engineering, plant breeding, and general plant biotechnology.

Plant Protoplasts and Genetic Engineering III

Author : Y. P. S. Bajaj
Publisher : Springer Science & Business Media
ISBN 13 : 3642780067
Total Pages : 349 pages
Book Rating : 4.6/5 (427 download)

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Book Synopsis Plant Protoplasts and Genetic Engineering III by : Y. P. S. Bajaj

Download or read book Plant Protoplasts and Genetic Engineering III written by Y. P. S. Bajaj and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 349 pages. Available in PDF, EPUB and Kindle. Book excerpt: In continuation of Volumes 8 and 9 (1989) on in vitro manipulation of plant protoplasts, this new volume deals with the regeneration of plants from protoplasts and genetic transformation in various species of Agrostis, Arabidopsis, Atropa, Brassica, Catharanthus, Datura, Cucumis, Daucus, Digitalis, Duboisia, Eustoma, Festuca, Helianthus, Hordeum, Kalanchoe, Linum, Lobelia, Lolium, Lotus, Lycium, Lycopersicum, Mentha, Nicotiana, Pelargonium, Pisum, Pyrus, Salvia, Scopolia, and Solanum.These studies reflect the far reaching implications of protoplast technologyin genetic engineering of plants. They are of special interest to researchers in the field of plant tissue culture, molecular biology, genetic engineering, and plant breeding.

Plant Protoplasts and Genetic Engineering V

Author : Professor Dr. Y. P. S. Bajaj
Publisher : Springer Science & Business Media
ISBN 13 : 3662093669
Total Pages : 432 pages
Book Rating : 4.6/5 (62 download)

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Book Synopsis Plant Protoplasts and Genetic Engineering V by : Professor Dr. Y. P. S. Bajaj

Download or read book Plant Protoplasts and Genetic Engineering V written by Professor Dr. Y. P. S. Bajaj and published by Springer Science & Business Media. This book was released on 2013-03-14 with total page 432 pages. Available in PDF, EPUB and Kindle. Book excerpt: In continuation of Volumes 8, 9, 22, and 23, this new volume deals with the regeneration of plants from isolated protoplasts and genetic transformation in various species of Actinidia, Allocasuarina, Anthurium, Antirrhinum, Asparagus, Beta, Brassica, Carica, Casuarina, Cyphomandra, Eucalyptus, Ipomoea, Larix, Limonium, Liriodendron, Malus, Musa, Physcomitrella, Physalis, Picea, Rosa, Tagetes, Triticum, and Ulmus. These studies reflect the far-reaching implications of protoplast technology in genetic engineering of plants. The book contains a wealth of useful information for advanced students, teachers, and researchers in the field of plant tissue culture, molecular biology, genetic engineering, plant breeding, and general biotechnology.

Plant Protoplasts

Author : Fowke
Publisher : CRC Press
ISBN 13 : 1351084224
Total Pages : 254 pages
Book Rating : 4.3/5 (51 download)

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Book Synopsis Plant Protoplasts by : Fowke

Download or read book Plant Protoplasts written by Fowke and published by CRC Press. This book was released on 2018-01-18 with total page 254 pages. Available in PDF, EPUB and Kindle. Book excerpt: The present book thus emphasizes the importance of plant protoplasts for fundamental research. This book is intended to be used b senior undergraduates, graduate students, and research scientists in plant biology.

Genetic Engineering of Plants

Author : National Research Council
Publisher : National Academies Press
ISBN 13 : 0309034345
Total Pages : 97 pages
Book Rating : 4.3/5 (9 download)

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Book Synopsis Genetic Engineering of Plants by : National Research Council

Download or read book Genetic Engineering of Plants written by National Research Council and published by National Academies Press. This book was released on 1984-02-01 with total page 97 pages. Available in PDF, EPUB and Kindle. Book excerpt: "The book...is, in fact, a short text on the many practical problems...associated with translating the explosion in basic biotechnological research into the next Green Revolution," explains Economic Botany. The book is "a concise and accurate narrative, that also manages to be interesting and personal...a splendid little book." Biotechnology states, "Because of the clarity with which it is written, this thin volume makes a major contribution to improving public understanding of genetic engineering's potential for enlarging the world's food supply...and can be profitably read by practically anyone interested in application of molecular biology to improvement of productivity in agriculture."

Plant Protoplasts and Genetic Engineering I

Author : Y. P. S. Bajaj
Publisher : Springer Science & Business Media
ISBN 13 : 3642736149
Total Pages : 461 pages
Book Rating : 4.6/5 (427 download)

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Book Synopsis Plant Protoplasts and Genetic Engineering I by : Y. P. S. Bajaj

Download or read book Plant Protoplasts and Genetic Engineering I written by Y. P. S. Bajaj and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 461 pages. Available in PDF, EPUB and Kindle. Book excerpt: Isolated protoplasts are a unique tool for genetic manipulation of plants. Since the discovery of a method for the enzymatic isolation of pro-· toplasts by Professor E. C. Cocking in 1960, tremendous progress has been made in this very fascinating area of research. I have witnessed the struggle in the 1960's and early 1970's, when obtaining a clean prepara tion of protoplasts was considered an achievement. I also shared the pioneering excitement and enthusiasm in this field during the 2nd Inter national Congress of Plant Tissue Culture held at Strasbourg in 1970, where Dr. I. Thkebe of Japan presented his work on the induction of division in tobacco protoplasts. This was followed by my participation in three international conferences devoted to plant protoplasts held in 1972 in Salamanca (Spain) and Versailles (France), and then in 1975 in Nottingham (England). The enthusiasm shown by plant scientists at these meetings was ample proof of the bright future of protoplast technology, and it became evident that protoplasts would playa major role in plant biotechnology, especially in genetic engineering. Since then we have never looked back, and now the methods for isolation, fusion, and culture, as well as regeneration of somatic hybrids, have become routine laboratory procedures for most plant species. Currently the focus is on cereal and tree protoplasts. In order to bring about any genetic manipulation through fusion, in corporation of DNA, and transformation, the regeneration of the entire plant through manipulation of protoplasts is a prerequisite.

Safety of Genetically Engineered Foods

Author : National Research Council
Publisher : National Academies Press
ISBN 13 : 0309166152
Total Pages : 254 pages
Book Rating : 4.3/5 (91 download)

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Book Synopsis Safety of Genetically Engineered Foods by : National Research Council

Download or read book Safety of Genetically Engineered Foods written by National Research Council and published by National Academies Press. This book was released on 2004-07-08 with total page 254 pages. Available in PDF, EPUB and Kindle. Book excerpt: Assists policymakers in evaluating the appropriate scientific methods for detecting unintended changes in food and assessing the potential for adverse health effects from genetically modified products. In this book, the committee recommended that greater scrutiny should be given to foods containing new compounds or unusual amounts of naturally occurring substances, regardless of the method used to create them. The book offers a framework to guide federal agencies in selecting the route of safety assessment. It identifies and recommends several pre- and post-market approaches to guide the assessment of unintended compositional changes that could result from genetically modified foods and research avenues to fill the knowledge gaps.

Agrobacterium biology and its application to transgenic plant production

Author : Hau-Hsuan Hwang
Publisher : Frontiers Media SA
ISBN 13 : 2889195740
Total Pages : 167 pages
Book Rating : 4.8/5 (891 download)

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Book Synopsis Agrobacterium biology and its application to transgenic plant production by : Hau-Hsuan Hwang

Download or read book Agrobacterium biology and its application to transgenic plant production written by Hau-Hsuan Hwang and published by Frontiers Media SA. This book was released on 2015-06-26 with total page 167 pages. Available in PDF, EPUB and Kindle. Book excerpt: The broad host range pathogenic bacterium Agrobacterium tumefaciens has been widely studied as a model system to understand horizontal gene flow, secretion of effector proteins into host cells, and plant-pathogen interactions. Agrobacterium-mediated plant transformation also is the major method for generating transgenic plants for research and biotechnology purposes. Agrobacterium species have the natural ability to conduct interkingdom genetic transfer from bacteria to eukaryotes, including most plant species, yeast, fungi, and even animal cells. In nature, A. tumefaciens causes crown gall disease resulting from expression in plants of auxin and cytokinin biosynthesis genes encoded by the transferred (T-) DNA. Gene transfer from A. tumefaciens to host cells requires virulence (vir) genes that reside on the resident tumor-inducing (Ti) plasmid. In addition to T-DNA, several Virulence (Vir) effector proteins are also translocated to host cells through a bacterial type IV secretion system. These proteins aid in T-DNA trafficking through the host cell cytoplasm, nuclear targeting, and T-DNA integration. Genes within native T-DNAs can be replaced by any gene of interest, making Agrobacterium species important tools for plant research and genetic engineering. In this research topic, we provided updated information on several important areas of Agrobacterium biology and its use for biotechnology purposes.

Transgenic Microalgae as Green Cell Factories

Author : Rosa León
Publisher : Springer Science & Business Media
ISBN 13 : 0387755322
Total Pages : 145 pages
Book Rating : 4.3/5 (877 download)

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Book Synopsis Transgenic Microalgae as Green Cell Factories by : Rosa León

Download or read book Transgenic Microalgae as Green Cell Factories written by Rosa León and published by Springer Science & Business Media. This book was released on 2008-12-10 with total page 145 pages. Available in PDF, EPUB and Kindle. Book excerpt: Microalgae have been largely commercialized as food and feed additives, and their potential as a source of high-added value compounds is well known. Yet, only a few species of microalgae have been genetically transformed with efficiency. A better understanding of the mechanisms that control the regulation of gene expression in eukaryotes is therefore needed. In this book a group of outstanding researchers working on different areas of microalgae biotechnology offer a global vision of the genetic manipulation of microalgae and their applications.

Gene Transfer to Plants

Author : Ingo Potrykus
Publisher : Springer Science & Business Media
ISBN 13 : 3642792472
Total Pages : 370 pages
Book Rating : 4.6/5 (427 download)

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Book Synopsis Gene Transfer to Plants by : Ingo Potrykus

Download or read book Gene Transfer to Plants written by Ingo Potrykus and published by Springer Science & Business Media. This book was released on 2013-06-29 with total page 370 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Biolistic DNA Delivery in Plants

Author : Sachin Rustgi
Publisher : Humana
ISBN 13 : 9781071603581
Total Pages : 334 pages
Book Rating : 4.6/5 (35 download)

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Book Synopsis Biolistic DNA Delivery in Plants by : Sachin Rustgi

Download or read book Biolistic DNA Delivery in Plants written by Sachin Rustgi and published by Humana. This book was released on 2021-04-25 with total page 334 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume details protocols for the use of the biolistic DNA delivery method in different plant species. Chapters guide readers through non-protocol chapters that cover relevant topics of interest, a broad overview of the field, exciting modifications of the system, and reliable plant transformation procedures in different plant species. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Biolistic DNA Delivery: Methods and Protocols aims to provide a comprehensive collection of protocols to intended to be a practical guide for the novice as well as the advanced user in the field of plant genetic transformation.

The Molecular Biology of Plant Cells

Author : H. Smith
Publisher : Univ of California Press
ISBN 13 : 9780520034655
Total Pages : 516 pages
Book Rating : 4.0/5 (346 download)

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Book Synopsis The Molecular Biology of Plant Cells by : H. Smith

Download or read book The Molecular Biology of Plant Cells written by H. Smith and published by Univ of California Press. This book was released on 1977-01-01 with total page 516 pages. Available in PDF, EPUB and Kindle. Book excerpt: Plant cell structure and function; Gene expression and its regulation in plant cells; The manipulation of plant cells.

Plant Transformation Technologies

Author : Charles Neal Stewart
Publisher : John Wiley & Sons
ISBN 13 : 0470958944
Total Pages : 314 pages
Book Rating : 4.4/5 (79 download)

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Book Synopsis Plant Transformation Technologies by : Charles Neal Stewart

Download or read book Plant Transformation Technologies written by Charles Neal Stewart and published by John Wiley & Sons. This book was released on 2011-01-31 with total page 314 pages. Available in PDF, EPUB and Kindle. Book excerpt: Plant Transformation Technologies is a comprehensive, authoritative book focusing on cutting-edge plant biotechnologies, offering in-depth, forward-looking information on methods for controlled and accurate genetic engineering. In response to ever-increasing pressure for precise and efficient integration of transgenes in plants, many new technologies have been developed. With complete coverage of these technologies, Plant Transformation Technologies provides valuable insight on current and future plant transformation technologies. With twenty-five chapters written by international experts on transformation technologies, the book includes new information on Agrobacterium, targeting transgenes into plant genomes, and new vectors and market systems. Including both review chapters and protocols for transformation, Plant Transformation Technologies is vitally important to graduate students, postdoctoral students, and university and industry researchers.

Experimental Manipulation of Gene Expression

Author : Masayori Inouye
Publisher : Academic Press
ISBN 13 : 1483273970
Total Pages : 330 pages
Book Rating : 4.4/5 (832 download)

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Book Synopsis Experimental Manipulation of Gene Expression by : Masayori Inouye

Download or read book Experimental Manipulation of Gene Expression written by Masayori Inouye and published by Academic Press. This book was released on 2014-06-28 with total page 330 pages. Available in PDF, EPUB and Kindle. Book excerpt: Experimental Manipulation of Gene Expression discusses a wide range of host systems in which to clone and express a gene of interest. The aims are for readers to quickly learn the versatility of the systems and obtain an overview of the technology involved in the manipulation of gene expression. Furthermore, it is hoped that the reader will learn enough from the various approaches to be able to develop systems and to arrange for a gene of particular interest to express in a particular system. The book opens with a chapter on the design and construction of a plasmid vector system used to achieve high-level expression of a particular phage regulatory protein normally found in minute amounts in a phage-infected bacterial cell. This is followed by separate chapters on topics such as high-level expression vectors that utilize efficient Escherichia coli lipoprotein promoter as well as various other portions of the lipoprotein gene Ipp; DNA cloning systems for streptomycetes; and the design and application of vectors for high-level, inducible synthesis of the product of a cloned gene in yeast.

Biotechnology and the Food Supply

Author : National Research Council
Publisher : National Academies
ISBN 13 :
Total Pages : 120 pages
Book Rating : 4.1/5 (497 download)

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Book Synopsis Biotechnology and the Food Supply by : National Research Council

Download or read book Biotechnology and the Food Supply written by National Research Council and published by National Academies. This book was released on 1988-01-01 with total page 120 pages. Available in PDF, EPUB and Kindle. Book excerpt: Biotechnology and the food supply was the subject of the Food and Nutrition Board's annual symposium. The papers presented at the symposium, and contained in this volume, address various aspects of this topic, including food production, food safety, and food quality.