Development Of Methods For Directed And Structured Immobilization Of Biomolecules

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Development of Methods for Directed and Structured Immobilization of Biomolecules

Author : Björn Waterkotte
Publisher : Cuvillier Verlag
ISBN 13 : 3736945329
Total Pages : 252 pages
Book Rating : 4.7/5 (369 download)

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Book Synopsis Development of Methods for Directed and Structured Immobilization of Biomolecules by : Björn Waterkotte

Download or read book Development of Methods for Directed and Structured Immobilization of Biomolecules written by Björn Waterkotte and published by Cuvillier Verlag. This book was released on 2013-11-07 with total page 252 pages. Available in PDF, EPUB and Kindle. Book excerpt: Surface immobilized molecules play a crucial role in applied as well as in basic research. They can be found in DNA or protein-array as well as in cell-migration or biochemical interaction experiments. Especially for proteins the immobilization can critically affect molecule accessibility and activity. In this work two essential aspects of protein immobilization were investigated: the identification of suitable surface functionalization by two array approaches and the generation of laterally structured gray scale protein patterns by projection lithography. In proof-of-concept experiments a new strategy to create an array of different, neighboring functionalizations on the same surface was successfully tested. In "mechanical protection" inert plungers were applied to protect distinct surface areas from the surface functionalization reagents and allowed a selective modification of individual regions in serial reactions. In a particle based alternative approach a high spot density surface array was fabricated using functionalized, polyvinyl acetate, melamine and silicon oxide-based particles. The in situ encoded bead-based array (IEBA) allowed the study of immobilization conditions and the effect of protein adhesion. For the reliable and fast analysis of array-images with several thousand individual particles a custom open-source evaluation software was developed. To create protein patterns a custom-built projection lithography device was utilized to generate high definition protein patterns. Patterning conditions were optimized with respect to deposition speed and signal to noise ratio while retaining a biocompatible process flow. The established technology provides protein patterns with great control over the deposited surface density, patterns were transferred within seconds to minutes to functionalized glass slides. To obtain three-dimensional, surface-functionalized surfaces this approach was transferred to thin polymer films. The substrates were demonstrated to be biocompatible with mouse fibroblasts. These surfaces allow a novel level of control addressing both the chemical surrounding and the shape of a cell microenvironment.

Bioconjugate Techniques

Author : Greg T. Hermanson
Publisher : Academic Press
ISBN 13 : 0080568726
Total Pages : 1233 pages
Book Rating : 4.0/5 (85 download)

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Book Synopsis Bioconjugate Techniques by : Greg T. Hermanson

Download or read book Bioconjugate Techniques written by Greg T. Hermanson and published by Academic Press. This book was released on 2010-07-26 with total page 1233 pages. Available in PDF, EPUB and Kindle. Book excerpt: Bioconjugate Techniques, 2nd Edition, is the essential guide to the modification and cross linking of biomolecules for use in research, diagnostics, and therapeutics. It provides highly detailed information on the chemistry, reagent systems, and practical applications for creating labeled or conjugate molecules. It also describes dozens of reactions with details on hundreds of commercially available reagents and the use of these reagents for modifying or cross linking peptides and proteins, sugars and polysaccharides, nucleic acids and oligonucleotides, lipids, and synthetic polymers. A one-stop source for proven methods and protocols for synthesizing bioconjugates in the lab Step-by-step presentation makes the book an ideal source for researchers who are less familiar with the synthesis of bioconjugates More than 600 figures that visually describe the complex reactions associated with the synthesis of bioconjugates Includes entirely new chapters on the latest areas in the field of bioconjugation as follows: Microparticles and nanoparticlesSilane coupling agentsDendrimers and dendronsChemoselective ligationQuantum dotsLanthanide chelatesCyanine dyesDiscrete PEG compoundsBuckyballs,fullerenes, and carbon nanotubesMass tags and isotope tagsBioconjugation in the study of protein interactions

Surface Modification of Biopolymers

Author : Vijay Kumar Thakur
Publisher : John Wiley & Sons
ISBN 13 : 1119044774
Total Pages : 448 pages
Book Rating : 4.1/5 (19 download)

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Book Synopsis Surface Modification of Biopolymers by : Vijay Kumar Thakur

Download or read book Surface Modification of Biopolymers written by Vijay Kumar Thakur and published by John Wiley & Sons. This book was released on 2015-05-26 with total page 448 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book addresses surface modification techniques, which are critical for tailoring and broadening the applications of naturally occurring biopolymers. Biopolymers represent a sustainable solution to the need for new materials in the auto, waste removal, biomedical device, building material, defense, and paper industries. Features: First comprehensive summary of biopolymer modification methods to enhance compatibility, flexibility, enhanced physicochemical properties, thermal stability, impact response, and rigidity, among others Address of a green, eco-friendly materials that is increasing in use, underscoring the roles of material scientists in the future of new "green" bioolymer material use Coverage applications in automotive development, hazardous waste removal, biomedical engineering, pulp and paper industries, development of new building materials, and defense-related technologies Facilitation of technology transfer

9th International Conference on the Development of Biomedical Engineering in Vietnam

Author : Van Toi Vo
Publisher : Springer Nature
ISBN 13 : 3031446305
Total Pages : 1140 pages
Book Rating : 4.0/5 (314 download)

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Book Synopsis 9th International Conference on the Development of Biomedical Engineering in Vietnam by : Van Toi Vo

Download or read book 9th International Conference on the Development of Biomedical Engineering in Vietnam written by Van Toi Vo and published by Springer Nature. This book was released on 2023-11-19 with total page 1140 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book presents cutting-edge research and developments in the field of biomedical engineering, with a special emphasis on results achieved in Vietnam and neighboring low- and middle-income countries. Covering both fundamental and applied research, and focusing on the theme of “Translational Healthcare Technology from Advanced to Low and Middle Income Countries in the Era of Covid and Digital Transformation”, it reports on the design, fabrication, and application of low-cost and portable medical devices, biosensors, and microfluidic devices, on improved methods for biological data acquisition and analysis, on nanoparticles for biological applications, and on new achievements in biomechanics, tissue engineering, and regeneration. It describes the developments of molecular and cellular biology techniques, neuroengineering techniques, and statistical and computational methods, including artificial intelligence, for biomedical applications. It also discusses strategies to address some relevant issues in biomedical education and entrepreneurship. Gathering the proceedings of the 9th International Conference on The Development of Biomedical Engineering in Vietnam, BME 9, held on December 27-29, 2022, in Ho Chi Minh, Vietnam, the book offers important answers to current challenges in the field and a source of inspiration for scientists, engineers, and researchers with various backgrounds working in different research institutes, companies, and countries.

National Marine Pollution Program

Author :
Publisher :
ISBN 13 :
Total Pages : 718 pages
Book Rating : 4.:/5 (318 download)

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Book Synopsis National Marine Pollution Program by :

Download or read book National Marine Pollution Program written by and published by . This book was released on 1986 with total page 718 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Biocatalysis: Biochemical Fundamentals And Applications (Second Edition)

Author : Peter Grunwald
Publisher : World Scientific Publishing Company
ISBN 13 : 1783269103
Total Pages : 1314 pages
Book Rating : 4.7/5 (832 download)

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Book Synopsis Biocatalysis: Biochemical Fundamentals And Applications (Second Edition) by : Peter Grunwald

Download or read book Biocatalysis: Biochemical Fundamentals And Applications (Second Edition) written by Peter Grunwald and published by World Scientific Publishing Company. This book was released on 2017-08-04 with total page 1314 pages. Available in PDF, EPUB and Kindle. Book excerpt: In this Completely Revised and Extended Edition with a significantly enhanced content, all Chapters have been updated considering relevant literature and recent developments until 2016 together with application oriented examples with a focus on Industrial Biocatalysis. Newly treated topics comprise among others systems metabolic engineering approaches, metagenome screening, new tools for pathway engineering, and de-novo computational design as actual research areas in biocatalysis. Information about different aspects of RNA technologies, and completely new Chapters on 'Fluorescent Proteins' and 'Biocatalysis and Nanotechnology' are also included.

Nanobiosensors for Biomolecular Targeting

Author : Subash C.B. Gopinath
Publisher : Elsevier
ISBN 13 : 9780128139004
Total Pages : 0 pages
Book Rating : 4.1/5 (39 download)

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Book Synopsis Nanobiosensors for Biomolecular Targeting by : Subash C.B. Gopinath

Download or read book Nanobiosensors for Biomolecular Targeting written by Subash C.B. Gopinath and published by Elsevier. This book was released on 2018-10-23 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Nanobiosensors for Bio-molecular Targeting presents the latest analytical methods for the detection of different substances in the range of small molecules to whole cells, exploring the advantages and disadvantages of each method. Biosensors combine the component of biological origin and physicochemical detector to show the presence of analytes in a given sample. The use of bionanotechnology has led to a significant advancement in the progression of nanobiosensors and has been effectively used for biomedical diagnosis.

Summary of Federal Programs and Projects

Author : United States. National Ocean Pollution Program Office
Publisher :
ISBN 13 :
Total Pages : 454 pages
Book Rating : 4.E/5 ( download)

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Book Synopsis Summary of Federal Programs and Projects by : United States. National Ocean Pollution Program Office

Download or read book Summary of Federal Programs and Projects written by United States. National Ocean Pollution Program Office and published by . This book was released on 1991 with total page 454 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Nanotechnology and Biosensors

Author : Dimitrios P Nikolelis
Publisher : Elsevier
ISBN 13 : 0128138858
Total Pages : 474 pages
Book Rating : 4.1/5 (281 download)

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Book Synopsis Nanotechnology and Biosensors by : Dimitrios P Nikolelis

Download or read book Nanotechnology and Biosensors written by Dimitrios P Nikolelis and published by Elsevier. This book was released on 2018-06-27 with total page 474 pages. Available in PDF, EPUB and Kindle. Book excerpt: Nanotechnology and Biosensors shows how nanotechnology is used to create affordable, mass-produced, portable, small sized biosensors to directly monitor environmental pollutants. In addition, it provides information on their integration into components and systems for mass market applications in food analysis, environmental monitoring and health diagnostics. Nanotechnology has led to a dramatic improvement in the performance, sensitivity and selectivity of biosensors. As metal-oxide and carbon nanostructures, gold and magnetite nanoparticles, and the integration of dendrimers in biosensors using nanotechnology have contributed greatly in making biosensors more effective and affordable on a mass-market level, this book presents a timely resource on the topic. - Highlights nanotechnology-based approaches to the detection of enzyme inhibitors, direct enzymatic and microbial detection of metabolites, and nutrients using biosensors - Includes examples on how nanotechnology has lead to improvements in the construction of portable, selective and sensitive biosensing devices - Offers thorough coverage of biomarker/biosensor interaction for the rapid detection of toxicants and pollutants

Immobilized Biomolecules in Analysis

Author : A. E. G. Cass
Publisher : Practical Approach
ISBN 13 : 9780199636365
Total Pages : 254 pages
Book Rating : 4.6/5 (363 download)

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Book Synopsis Immobilized Biomolecules in Analysis by : A. E. G. Cass

Download or read book Immobilized Biomolecules in Analysis written by A. E. G. Cass and published by Practical Approach. This book was released on 1998 with total page 254 pages. Available in PDF, EPUB and Kindle. Book excerpt: Biomolecules and cells are critical components of biosensors and biomaterials, but in order to function in an artificial environment, they must be immobilized in a manner that does not affect their interaction with target analytes. Biosensors demonstrate that we can harness the incrediblefunctions of living molecules and cells for our own purposes and are therefore at the forefront of technology. Moreover the applications of immobilized biomolecules and cells are expected to expand far beyond biosensor applications and indeed are already used for pharmaceutical production andtesting. Biomaterials will become increasing common as they are being developed into toxic filters, artificial organs, and even silicon chips. This book provides a selection of methods for the immobilization of biomolecules and cells on a variety of surface with different geometries and chemistriesso that they retain their function and guidelines on which method to use. Also included are the analytical techniques to measure the functionality of immobilized biomolecules. All the protocols have been tried and validated by the authors. Immobilized Biomolecules in Analysis: A Practical Approachis an invaluable guide to all researchers in the fields of biosensors and biomaterials. Research in biosensors is carried out in a wide variety of fields including biochemistry, chemistry, engineering, laboratory medicine, environmental and defence research. The protocols are written so that anextensive prior knowledge of biochemistry is not required to use them.

Biomolecular Electronics

Author : Paolo Facci
Publisher : William Andrew
ISBN 13 : 1455731528
Total Pages : 257 pages
Book Rating : 4.4/5 (557 download)

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Book Synopsis Biomolecular Electronics by : Paolo Facci

Download or read book Biomolecular Electronics written by Paolo Facci and published by William Andrew. This book was released on 2014-04-03 with total page 257 pages. Available in PDF, EPUB and Kindle. Book excerpt: Biomolecular Electronics – the electrical control of biological phenomena – is a scientific challenge that, once fully realized, will find a wide range of applications from electronics and computing to medicine and therapeutic techniques.This new arena of biomolecular electronics is approached using familiar concepts from many areas such as electrochemistry, device electronics and some mechanisms of gene expression level control. Practical techniques are explored by which electrical and electronic means can be used to control biological reactions and processes. Also, the current and future applications for this new and expanding field are discussed.This book is aimed at scientists and engineers involved in both research and commercial applications across fields including bioelectronics, bionanotechnology, electrochemistry and nanomedicine – providing a state-of-the-art survey of what's going on at the boundary between biology and electronic technology at the micro- and nano- scales, along with a suggestive insight into future possible developments. - Demystifies the science and applications of electrically-driven biological reactions - Explains how the techniques of bioelectronics and electrochemistry can be deployed as biological control technologies - Provides applications information for diverse areas from bio-electrochemistry to electrical control of gene expression levels

Biocatalyst Immobilization

Author : Maria Lujan Ferreira
Publisher : Academic Press
ISBN 13 : 0323913776
Total Pages : 460 pages
Book Rating : 4.3/5 (239 download)

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Book Synopsis Biocatalyst Immobilization by : Maria Lujan Ferreira

Download or read book Biocatalyst Immobilization written by Maria Lujan Ferreira and published by Academic Press. This book was released on 2022-11-12 with total page 460 pages. Available in PDF, EPUB and Kindle. Book excerpt: Biocatalyst Immobilization: Foundations and Applications provides a comprehensive overview of biocatalytic immobilization processes, as well as methods for study, characterization and application. Early chapters discuss current progress in enzyme immobilization and methods for selecting and pretreating enzymes prior to immobilization, with an emphasis on navigating common challenges and employing enzyme supports and post immobilization treatments to impact enzymatic activity. Process-based chapters instruct on measuring and reporting on enzyme immobilization efficiency, protein final content, quantification of reaction products, and the use of nanomaterials to characterize immobilized enzymes. Later chapters examine recent advances, including novel enzymatic reactors, multi-enzymatic biocatalysts, enzymatic biosensors, whole cell immobilization, the industrial application of immobilized enzymes, and perspectives on future trends. - Provides a thorough overview of biocatalyst and enzyme immobilization for research and practical application - Presents methods based content that instructs in enzyme immobilization pretreatment, enzyme supports, post immobilization treatments, measuring enzyme immobilization efficiency, quantification of reaction products, and whole cell immobilization - Features chapter contributions from international leaders in the field

Handbook of Nanostructured Materials and Nanotechnology, Five-Volume Set

Author : Hari Singh Nalwa
Publisher : Academic Press
ISBN 13 : 0080533647
Total Pages : 3593 pages
Book Rating : 4.0/5 (85 download)

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Book Synopsis Handbook of Nanostructured Materials and Nanotechnology, Five-Volume Set by : Hari Singh Nalwa

Download or read book Handbook of Nanostructured Materials and Nanotechnology, Five-Volume Set written by Hari Singh Nalwa and published by Academic Press. This book was released on 1999-10-29 with total page 3593 pages. Available in PDF, EPUB and Kindle. Book excerpt: Nanostructured materials is one of the hottest and fastest growing areas in today's materials science field, along with the related field of solid state physics. Nanostructured materials and their based technologies have opened up exciting new possibilites for future applications in a number of areas including aerospace, automotive, x-ray technology, batteries, sensors, color imaging, printing, computer chips, medical implants, pharmacy, and cosmetics. The ability to change properties on the atomic level promises a revolution in many realms of science and technology. Thus, this book details the high level of activity and significant findings are available for those involved in research and development in the field. It also covers industrial findings and corporate support. This five-volume set summarizes fundamentals of nano-science in a comprehensive way. The contributors enlisted by the editor are at elite institutions worldwide. Key Features * Provides comprehensive coverage of the dominant technology of the 21st century * Written by 127 authors from 16 countries, making this truly international * First and only reference to cover all aspects of nanostructured materials and nanotechnology

Technologies for Smart Sensors and Sensor Fusion

Author : Kevin Yallup
Publisher : CRC Press
ISBN 13 : 135183147X
Total Pages : 492 pages
Book Rating : 4.3/5 (518 download)

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Book Synopsis Technologies for Smart Sensors and Sensor Fusion by : Kevin Yallup

Download or read book Technologies for Smart Sensors and Sensor Fusion written by Kevin Yallup and published by CRC Press. This book was released on 2017-12-19 with total page 492 pages. Available in PDF, EPUB and Kindle. Book excerpt: Exciting new developments are enabling sensors to go beyond the realm of simple sensing of movement or capture of images to deliver information such as location in a built environment, the sense of touch, and the presence of chemicals. These sensors unlock the potential for smarter systems, allowing machines to interact with the world around them in more intelligent and sophisticated ways. Featuring contributions from authors working at the leading edge of sensor technology, Technologies for Smart Sensors and Sensor Fusion showcases the latest advancements in sensors with biotechnology, medical science, chemical detection, environmental monitoring, automotive, and industrial applications. This valuable reference describes the increasingly varied number of sensors that can be integrated into arrays, and examines the growing availability and computational power of communication devices that support the algorithms needed to reduce the raw sensor data from multiple sensors and convert it into the information needed by the sensor array to enable rapid transmission of the results to the required point. Using both SI and US units, the text: Provides a fundamental and analytical understanding of the underlying technology for smart sensors Discusses groundbreaking software and sensor systems as well as key issues surrounding sensor fusion Exemplifies the richness and diversity of development work in the world of smart sensors and sensor fusion Offering fresh insight into the sensors of the future, Technologies for Smart Sensors and Sensor Fusion not only exposes readers to trends but also inspires innovation in smart sensor and sensor system development.

Zinc Oxide Nanostructures: Synthesis and Characterization

Author : Sotirios Baskoutas
Publisher : MDPI
ISBN 13 : 3038973025
Total Pages : 303 pages
Book Rating : 4.0/5 (389 download)

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Book Synopsis Zinc Oxide Nanostructures: Synthesis and Characterization by : Sotirios Baskoutas

Download or read book Zinc Oxide Nanostructures: Synthesis and Characterization written by Sotirios Baskoutas and published by MDPI. This book was released on 2018-12-04 with total page 303 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book is a printed edition of the Special Issue "Zinc Oxide Nanostructures: Synthesis and Characterization" that was published in Materials

Conjugated Polymers for Next-Generation Applications, Volume 1

Author : Vijay Kumar
Publisher : Woodhead Publishing
ISBN 13 : 0128236345
Total Pages : 618 pages
Book Rating : 4.1/5 (282 download)

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Book Synopsis Conjugated Polymers for Next-Generation Applications, Volume 1 by : Vijay Kumar

Download or read book Conjugated Polymers for Next-Generation Applications, Volume 1 written by Vijay Kumar and published by Woodhead Publishing. This book was released on 2022-06-24 with total page 618 pages. Available in PDF, EPUB and Kindle. Book excerpt: Conjugated Polymers for Next-Generation Applications, Volume One: Synthesis, Properties and Optoelectrochemical Devices describes the synthesis and characterization of varied conjugated polymeric materials and their key applications, including active electrode materials for electrochemical capacitors and lithium-ion batteries, along with new ideas of functional materials for next-generation high-energy batteries, a discussion of common design procedures, and the pros and cons of conjugated polymers for certain applications. The book's emphasis lies in the underlying electronic properties of conjugated polymers, their characterization and analysis, and the evaluation of their effectiveness for utilization in energy and electronics applications. This book is ideal for researchers and practitioners in the area of materials science, chemistry and chemical engineering. - Provides an overview of the synthesis and functionalization of conjugated polymers and their composites - Reviews important photovoltaics applications of conjugated polymeric materials, including their use in energy storage, batteries and optoelectronic devices - Discusses conjugated polymers and their application in electronics for sensing, bioelectronics, memory, and more

Advances in Protein Molecular and Structural Biology Methods

Author : Timir Tripathi
Publisher : Academic Press
ISBN 13 : 0323902650
Total Pages : 716 pages
Book Rating : 4.3/5 (239 download)

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Book Synopsis Advances in Protein Molecular and Structural Biology Methods by : Timir Tripathi

Download or read book Advances in Protein Molecular and Structural Biology Methods written by Timir Tripathi and published by Academic Press. This book was released on 2022-01-14 with total page 716 pages. Available in PDF, EPUB and Kindle. Book excerpt: Advances in Protein Molecular and Structural Biology Methods offers a complete overview of the latest tools and methods applicable to the study of proteins at the molecular and structural level. The book begins with sections exploring tools to optimize recombinant protein expression and biophysical techniques such as fluorescence spectroscopy, NMR, mass spectrometry, cryo-electron microscopy, and X-ray crystallography. It then moves towards computational approaches, considering structural bioinformatics, molecular dynamics simulations, and deep machine learning technologies. The book also covers methods applied to intrinsically disordered proteins (IDPs)followed by chapters on protein interaction networks, protein function, and protein design and engineering. It provides researchers with an extensive toolkit of methods and techniques to draw from when conducting their own experimental work, taking them from foundational concepts to practical application. - Presents a thorough overview of the latest and emerging methods and technologies for protein study - Explores biophysical techniques, including nuclear magnetic resonance, X-ray crystallography, and cryo-electron microscopy - Includes computational and machine learning methods - Features a section dedicated to tools and techniques specific to studying intrinsically disordered proteins