Development of a novel real-time method to study the erythrocytic life cycle of Plasmodium falciparum using Quartz Crystal Microbalances

Download Development of a novel real-time method to study the erythrocytic life cycle of Plasmodium falciparum using Quartz Crystal Microbalances PDF Online Free

Author :
Publisher : Cuvillier Verlag
ISBN 13 : 3736932804
Total Pages : 158 pages
Book Rating : 4.7/5 (369 download)

DOWNLOAD NOW!


Book Synopsis Development of a novel real-time method to study the erythrocytic life cycle of Plasmodium falciparum using Quartz Crystal Microbalances by : Rosa Elena Bustos Cruz

Download or read book Development of a novel real-time method to study the erythrocytic life cycle of Plasmodium falciparum using Quartz Crystal Microbalances written by Rosa Elena Bustos Cruz and published by Cuvillier Verlag. This book was released on 2010-04-15 with total page 158 pages. Available in PDF, EPUB and Kindle. Book excerpt: Previous studies carried out by the Biosensor Research Group of the Institute of Clinical and Experimental Transfusion Medicine at the Tübingen University had successfully demonstrated determination of various blood types through the interaction of erythrocytes with immobilised antibodies on a QCM. These studies have opened the possibility to test new applications of this technique for the study of infectious diseases. In our case, studies of the erythrocyte life cycle of P. falciparum, particularly during the last six hours preceding the merozoite release and studies related to reinfection of by merozoites were the focus of our investigation. Release of malaria parasites of P. falciparum from infected erythrocyte at the end of their asexual erythrocyte cycle occurs approximately every 48 hours, asynchronously in parasite culture and synchronously in vivo. This process is poorly studied due to: (I) merozoite release is a very short event (60 S), (II) schizont stages have high sensitivity for culture conditions like pH, medium, osmotic pressure, gas atmosphere and temperature. (III) Schizont conditions are not easy to maintain under the miscrocope, making miscroscopical real time observation of the release difficult. The aim of the presented project was to study the release of Plasmodium falciparum merozoites from erythrocytes with the QCM sensor technique. In this way, the frequency shift due to the change of mass associated to the merozoite release and, on a second QCM, the reinfection of healthy erythrocytes are monitored in real time. Our QCM experiments included the following stages: (I) Adaptation and optimisation of the immobilisation of biological layers to capture the erythrocytes on the quartz. (II) Optimisation of parasite culture conditions in a QCM chamber. (III) Observation of the frequency signal both for infected and non-infected erythrocytes samples and correlation of the signal with the release of merozoites. (IV) Reinfection of healthy erythrocytes on a second quartz within one QCM system. (V) Test of inhibition of merozoite release and reinfection by antimalarial compounds. The results showed that there was significant increase of ~1000 Hz for QCM with infected erythrocyte compared to QCM with healthy erythrocytes, where the frequency remained stable. Microscopical observation of the quartz surface at different times during the experiment (TEM and optical) demonstrated a correlation between the increase in frequency and merozoite release. At this point, approximately more than 80% of the infected erythrocytes on the quartz are involved in the release. Reinfection of new erythrocytes was observed on a second QCM. pH of the system (7.2), Temperature (37°C+/-0.1), flow of the medium (9 µL/min), sterility of the process (BactAlert), gas atmosphere (O2 5%, residual N2) were established to ensure parasite development and survival. External controls using flow cytometry 24 hours after the reinfection show a parasitemia percentage of 1% in the erythrocytes infected in situ. Our results show, that the QCM technique is an appropriate and important new tool to elucidate the biology of the re-invasion process of Plasmodia.

Development of a Novel Real-time Method to Study the Erythrocytic Life Cycle of Plasmodium Falciparum Using Quartz Crystal Microbalances

Download Development of a Novel Real-time Method to Study the Erythrocytic Life Cycle of Plasmodium Falciparum Using Quartz Crystal Microbalances PDF Online Free

Author :
Publisher :
ISBN 13 :
Total Pages : 0 pages
Book Rating : 4.:/5 (845 download)

DOWNLOAD NOW!


Book Synopsis Development of a Novel Real-time Method to Study the Erythrocytic Life Cycle of Plasmodium Falciparum Using Quartz Crystal Microbalances by : Rosa Elena Bustos Cruz

Download or read book Development of a Novel Real-time Method to Study the Erythrocytic Life Cycle of Plasmodium Falciparum Using Quartz Crystal Microbalances written by Rosa Elena Bustos Cruz and published by . This book was released on 2010 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Quantitative Real-Time PCR

Download Quantitative Real-Time PCR PDF Online Free

Author :
Publisher :
ISBN 13 : 9781493998340
Total Pages : 235 pages
Book Rating : 4.9/5 (983 download)

DOWNLOAD NOW!


Book Synopsis Quantitative Real-Time PCR by : Roberto Biassoni

Download or read book Quantitative Real-Time PCR written by Roberto Biassoni and published by . This book was released on 2020 with total page 235 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book expands upon the useful first edition by exploring classic Quantitative Polymerase Chain Reaction (qPCR) techniques as well as a number of recently developed applications. With the changes in instrumentation due to technological advances and the development of new reagents to fulfill ethical and legal issues, the qPCR field is now an up-to-date technology that indeed is widely used in research and clinical diagnostics. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Revised and authoritative, Quantitative Real-Time PCR: Methods and Protocols, Second Edition is an ideal guide to this expanding and vital field of study.

Rapid Cycle Real-Time PCR - Methods and Applications

Download Rapid Cycle Real-Time PCR - Methods and Applications PDF Online Free

Author :
Publisher :
ISBN 13 : 9783642593987
Total Pages : 218 pages
Book Rating : 4.5/5 (939 download)

DOWNLOAD NOW!


Book Synopsis Rapid Cycle Real-Time PCR - Methods and Applications by : W. Dietmaier

Download or read book Rapid Cycle Real-Time PCR - Methods and Applications written by W. Dietmaier and published by . This book was released on 2014-01-15 with total page 218 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Microbes

Download Microbes PDF Online Free

Author :
Publisher : John Wiley & Sons
ISBN 13 : 1118311892
Total Pages : 731 pages
Book Rating : 4.1/5 (183 download)

DOWNLOAD NOW!


Book Synopsis Microbes by : Prakash S. Bisen

Download or read book Microbes written by Prakash S. Bisen and published by John Wiley & Sons. This book was released on 2012-06-22 with total page 731 pages. Available in PDF, EPUB and Kindle. Book excerpt: An accessible introduction to the world of microbes—from basic microbe biology through industrial applications Microbes affect our lives in a variety of ways—playing an important role in our health, food, agriculture, and environment. While some microbes are beneficial, others are pathogenic or opportunistic. Microbes: Concepts and Applications describes basic microbe biology and identification and shows not only how they operate in the subfields of medicine, biotechnology, environmental science, bioengineering, agriculture, and food science, but how they can be harnessed as a resource. It provides readers with a solid grasp of etiologic agents, pathogenic processes, epidemiology, and the role of microbes as therapeutic agents. Placing a major emphasis on omics technology, the book covers recent developments in the arena of microbes and discusses their role in industry and agriculture, as well as in related fields such as immunology, cell biology, and molecular biology. It offers complete discussions of the major bacterial, viral, fungal, and parasitic pathogens; includes information on emerging infectious diseases, antibiotic resistance, and bioterrorism; and talks about the future challenges in microbiology. The most complete treatment of microbial biology available, Microbes features eye-opening chapters on: Human and Microbial World Gene Technology: Application and Techniques Molecular Diagnostic and Medical Microbiology Identification and Classification of Microbes Diversity of Microorganisms Microbes in Agriculture Microbes as a Tool for Industry and Research Complete with charts and figures, this book is an invaluable textbook for university teachers, students, researchers, and people everywhere who care about microorganisms.

Redox-Active Therapeutics

Download Redox-Active Therapeutics PDF Online Free

Author :
Publisher : Springer
ISBN 13 : 3319307053
Total Pages : 694 pages
Book Rating : 4.3/5 (193 download)

DOWNLOAD NOW!


Book Synopsis Redox-Active Therapeutics by : Ines Batinić-Haberle

Download or read book Redox-Active Therapeutics written by Ines Batinić-Haberle and published by Springer. This book was released on 2016-10-13 with total page 694 pages. Available in PDF, EPUB and Kindle. Book excerpt: This essential volume comprehensively discusses redox-active therapeutics, focusing particularly on their molecular design, mechanistic, pharmacological and medicinal aspects. The first section of the book describes the basic aspects of the chemistry and biology of redox-active drugs and includes a brief overview of the redox-based pathways involved in cancer and the medical aspects of redox-active drugs, assuming little in the way of prior knowledge. Subsequent sections and chapters describe more specialized aspects of central nervous system injuries, neurodegenerative diseases, pain, radiation injury and radioprotection (such as of brain, lungs, head and neck and erectile function) and neglected diseases (e.g., leishmaniasis). It encompasses several major classes of redox-active experimental therapeutics, which include porphyrins, salens, nitrones, and most notably metal-containing (e.g., Mn, Fe, Cu, Zn, Sb) drugs as either single compounds or formulations with nanomaterials and quantum dots. Numerous illustrations, tables and figures enhance and complement the text; extensive references to relevant literature are also included. Redox-Active Therapeutics is an invaluable addition to Springer’s Oxidative Stress in Applied Basic Research and Clinical Practice series. It is essential reading for researchers, clinicians and graduate students interested in understanding and exploring the Redoxome—the organism redox network—as an emerging frontier in drug design, redox biology and medicine.

Dictionary of Medical Acronyms and Abbreviations

Download Dictionary of Medical Acronyms and Abbreviations PDF Online Free

Author :
Publisher : Hanley & Belfus
ISBN 13 : 9781560536512
Total Pages : pages
Book Rating : 4.5/5 (365 download)

DOWNLOAD NOW!


Book Synopsis Dictionary of Medical Acronyms and Abbreviations by : Stanley Jablonski

Download or read book Dictionary of Medical Acronyms and Abbreviations written by Stanley Jablonski and published by Hanley & Belfus. This book was released on 2004-09 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: This dictionary lists acronyms and abbreviations occurring with a reasonable frequency in the literature of medicine and the health care professions. Abbreviations and acronyms are given in capital letters, with no punctuation, and with concise definitions. The beginning sections also include symbols, genetic symbols, and the Greek alphabet and symbols.

Transcutaneous Bilirubinometry

Download Transcutaneous Bilirubinometry PDF Online Free

Author :
Publisher : Nova Science Publishers
ISBN 13 : 9781626182486
Total Pages : 0 pages
Book Rating : 4.1/5 (824 download)

DOWNLOAD NOW!


Book Synopsis Transcutaneous Bilirubinometry by : Daniele De Luca

Download or read book Transcutaneous Bilirubinometry written by Daniele De Luca and published by Nova Science Publishers. This book was released on 2013 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book has been written to summarise the available knowledge regarding transcutaneous bilirubinometry, the first technique that allows a completely non-invasive measurement of a clinical variable. This technique led to important changes in the management of neonatal jaundice, the most common clinical condition of new-born infants world-wide. Transcutaneous bilirubinometry has its roots in the old observation that yellow skin colour is due to the deposition of bilirubin, a chromophore molecule. More recently, transcutaneous bilirubinometry has involved many disciplines; and accordingly, this book has contributions from physicists, biologists, paediatric dermatologists and neonatologists who have clinical research expertise in the field of neonatal hyperbilirubinemia. We hope that this book may prompt the advancement of research that will answer the as-yet unanswered questions, improve the reliability and usefulness of transcutaneous bilirubinometry, and respond to the clinical needs of our little patients.

Concise Pocket Medical Dictionary

Download Concise Pocket Medical Dictionary PDF Online Free

Author :
Publisher : Anshan Pub
ISBN 13 : 9781905740918
Total Pages : 0 pages
Book Rating : 4.7/5 (49 download)

DOWNLOAD NOW!


Book Synopsis Concise Pocket Medical Dictionary by : U. N. Panda

Download or read book Concise Pocket Medical Dictionary written by U. N. Panda and published by Anshan Pub. This book was released on 2009 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: This concise and comprehensive dictionary, in four colour, gives information and definitions on medical terminology as required by students, nurses, pharmacists, laboratory and technical staff, health professionals, practitioners, paramedical and allied health workers. As well as the commonly used terms and vocabulary found in heavier volumes, this pocket dictionary also contains copious figures to illustrate key words. Every meaning is given with a simple, comprehensive definition. Particular attention is paid to the latest pharmaceuticals in the market, allowing a quick reading reference to the products of the 21st century. In 4 colour throughout for easy use, and containing a collection of useful appendices, the "Anshan Concise Pocket Medical Dictionary" truly is a handy, portable, reference book, which will serve students, academics, all types of medical and allied health professionals, and also anyone who wants to possess a reliable medical dictionary in their own home library.

Aseptolin

Download Aseptolin PDF Online Free

Author :
Publisher :
ISBN 13 :
Total Pages : 90 pages
Book Rating : 4.F/5 ( download)

DOWNLOAD NOW!


Book Synopsis Aseptolin by : Cyrus Edson

Download or read book Aseptolin written by Cyrus Edson and published by . This book was released on 1896 with total page 90 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Nanotechnology for Microfluidics

Download Nanotechnology for Microfluidics PDF Online Free

Author :
Publisher : John Wiley & Sons
ISBN 13 : 3527345337
Total Pages : 444 pages
Book Rating : 4.5/5 (273 download)

DOWNLOAD NOW!


Book Synopsis Nanotechnology for Microfluidics by : Xingyu Jiang

Download or read book Nanotechnology for Microfluidics written by Xingyu Jiang and published by John Wiley & Sons. This book was released on 2020-09-08 with total page 444 pages. Available in PDF, EPUB and Kindle. Book excerpt: The book focuses on microfluidics with applications in nanotechnology. The first part summarizes the recent advances and achievements in the field of microfluidic technology, with emphasize on the the influence of nanotechnology. The second part introduces various applications of microfluidics in nanotechnology, such as drug delivery, tissue engineering and biomedical diagnosis.

ToF-SIMS

Download ToF-SIMS PDF Online Free

Author :
Publisher : IM Publications
ISBN 13 : 1906715173
Total Pages : 742 pages
Book Rating : 4.9/5 (67 download)

DOWNLOAD NOW!


Book Synopsis ToF-SIMS by : J. C. Vickerman

Download or read book ToF-SIMS written by J. C. Vickerman and published by IM Publications. This book was released on 2013 with total page 742 pages. Available in PDF, EPUB and Kindle. Book excerpt: Time-of-flight secondary ion mass spectrometry (ToF-SIMS) is the most versatile of the surface analysis techniques that have been developed during the last 30 years. This is the Second Edition of the first book ToF-SIMS: Surface analysis by Mass Spectrometry to be dedicated to the subject and the treatment is comprehensive

Memlinc

Download Memlinc PDF Online Free

Author :
Publisher :
ISBN 13 :
Total Pages : 56 pages
Book Rating : 4.:/5 (8 download)

DOWNLOAD NOW!


Book Synopsis Memlinc by :

Download or read book Memlinc written by and published by . This book was released on 1905 with total page 56 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Alkane Functionalization

Download Alkane Functionalization PDF Online Free

Author :
Publisher : John Wiley & Sons
ISBN 13 : 111937880X
Total Pages : 680 pages
Book Rating : 4.1/5 (193 download)

DOWNLOAD NOW!


Book Synopsis Alkane Functionalization by : Armando J. L. Pombeiro

Download or read book Alkane Functionalization written by Armando J. L. Pombeiro and published by John Wiley & Sons. This book was released on 2019-03-11 with total page 680 pages. Available in PDF, EPUB and Kindle. Book excerpt: Presents state-of-the-art information concerning the syntheses of valuable functionalized organic compounds from alkanes, with a focus on simple, mild, and green catalytic processes Alkane Functionalization offers a comprehensive review of the state-of-the-art of catalytic functionalization of alkanes under mild and green conditions. Written by a team of leading experts on the topic, the book examines the latest research developments in the synthesis of valuable functionalized organic compounds from alkanes. The authors describe the various modes of interaction of alkanes with metal centres and examine theoxidative alkane functionalization upon C-O bond formation. They address the many types of mechanisms, discuss typical catalytic systems and highlight the strategies inspired by biological catalytic systems. The book also describes alkane functionalization upon C-heteroatom bond formation as well as oxidative and non-oxidative approaches. In addition, the book explores non-transition metal catalysts and metal-free catalytic systems and presents selected types of functionalization of sp3 C-H bonds pertaining to substrates other than alkanes. This important resource: Presents a guide to the most recent advances concerning the syntheses of valuable functionalized organic compounds from alkanes Contains information from leading experts on the topic Offers information on the catalytic functionalization of alkanes that allows for improved simplicity and sustainability compared to current multi-stage industrial processes Explores the challenges inherent with the application of alkanes as starting materials for syntheses of added value functionalized organic compounds Written for academic researchers and industrial scientists working in the fields of coordination chemistry, organometallic chemistry, catalysis, organic synthesis and green chemistry, Alkane Functionalization is an important resource for accessing the most up-to-date information available in the field of catalytic functionalization of alkanes.

Clinical Fluid Therapy in the Perioperative Setting

Download Clinical Fluid Therapy in the Perioperative Setting PDF Online Free

Author :
Publisher : Cambridge University Press
ISBN 13 : 1107119553
Total Pages : 309 pages
Book Rating : 4.1/5 (71 download)

DOWNLOAD NOW!


Book Synopsis Clinical Fluid Therapy in the Perioperative Setting by : Robert G. Hahn

Download or read book Clinical Fluid Therapy in the Perioperative Setting written by Robert G. Hahn and published by Cambridge University Press. This book was released on 2016-05-26 with total page 309 pages. Available in PDF, EPUB and Kindle. Book excerpt: The world's most renowned researchers in fluid management explain what you should know when providing infusion fluids to surgical patients.

Synthetic DNA Delivery Systems

Download Synthetic DNA Delivery Systems PDF Online Free

Author :
Publisher : Springer Science & Business Media
ISBN 13 : 9780306477010
Total Pages : 160 pages
Book Rating : 4.4/5 (77 download)

DOWNLOAD NOW!


Book Synopsis Synthetic DNA Delivery Systems by : Dan Luo

Download or read book Synthetic DNA Delivery Systems written by Dan Luo and published by Springer Science & Business Media. This book was released on 2003-09-30 with total page 160 pages. Available in PDF, EPUB and Kindle. Book excerpt: DNA delivery into cells is a rapidly developing area in gene therapy and biotechnology. Moreover, it is a powerful research tool to determine gene structure, regulation, and function. Viral methods of DNA delivery are well-characterized and efficient, but little is known about the toxicity and immunogenecity of viral vectors. As a result, non-viral, transfection methods of DNA delivery are of increasing interest. Synthetic DNA Delivery Systems is a comprehensive and current resource on DNA transfection. The use of histidine-rich peptides and polypeptides as DNA delivery systems and self-assembled delivery systems based on cationic lipids and polymers are discussed. Targeted delivery to organelles, tumor cells and dendritic cells comprise an important topic.

Coupling and Decoupling of Diverse Molecular Units in Glycosciences

Download Coupling and Decoupling of Diverse Molecular Units in Glycosciences PDF Online Free

Author :
Publisher : Springer
ISBN 13 : 9783319880624
Total Pages : 0 pages
Book Rating : 4.8/5 (86 download)

DOWNLOAD NOW!


Book Synopsis Coupling and Decoupling of Diverse Molecular Units in Glycosciences by : Zbigniew J. Witczak

Download or read book Coupling and Decoupling of Diverse Molecular Units in Glycosciences written by Zbigniew J. Witczak and published by Springer. This book was released on 2018-08-31 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: This unique book covers the latest developments in coupling and decoupling of biomolecules containing functionalized carbohydrate components, being one of the first collections in this important area of applied medicinal chemistry. Connecting molecules, often referred as bio-conjugation, has become one of the most often performed procedures in modern medicinal chemistry. Sometimes, when the connected molecules are not useful anymore, they must be disconnected. The molecules that must be connected (coupled) may belong to both small and large molecules and include such constructs as glycoproteins, glycopeptides and glycans. In this work, more than 15 experts address a comprehensive range of potential and current uses of in vitro and in vivo bio-conjugation methodologies, leading to a variety of glycoconjugates. The analytical aspects of bio-conjugation are also here discussed. Medicinal and organic chemists from graduate level onwards will understand the appeal of this important book.