Development And Broad Application Of A Mass Spectrometry Based Metabolomics Platform

Download Development And Broad Application Of A Mass Spectrometry Based Metabolomics Platform full books in PDF, epub, and Kindle. Read online Development And Broad Application Of A Mass Spectrometry Based Metabolomics Platform ebook anywhere anytime directly on your device. Fast Download speed and no annoying ads. We cannot guarantee that every ebooks is available!

Development and Broad Application of a Mass Spectrometry Based Metabolomics Platform

Author : Jay Steven Kirkwood
Publisher :
ISBN 13 :
Total Pages : 207 pages
Book Rating : 4.:/5 (859 download)

DOWNLOAD NOW!

Book Synopsis Development and Broad Application of a Mass Spectrometry Based Metabolomics Platform by : Jay Steven Kirkwood

Download or read book Development and Broad Application of a Mass Spectrometry Based Metabolomics Platform written by Jay Steven Kirkwood and published by . This book was released on 2013 with total page 207 pages. Available in PDF, EPUB and Kindle. Book excerpt: Metabolomics is a comprehensive analysis of small molecules, or metabolites, in a system. Metabolomics is a hypothesis-generating experiment and offers an unbiased analysis of cell metabolism that can aid in the understanding of fundamental biological processes. Metabolomics is widely and broadly applicable in the biological sciences and has been used to study gene function, elucidate mechanisms of drug action, develop novel therapeutics, and to better understand disease states. This dissertation presents studies aimed at developing and determining the suitability of a mass spectrometry based untargeted metabolomics platform. Four chapters of original research are presented in this dissertation. The first chapter describes the development of a liquid chromatography-quadrupole-time-of-flight mass spectrometry metabolomics platform and details the various steps involved in a typical metabolomics experiment including metabolite extraction/sample preparation, metabolite separation and data collection, data processing and statistical analysis, and metabolite identification. The second chapter applies the metabolomics platform to uncover the metabolic consequences of vitamin C deficiency in zebrafish, which, like humans cannot synthesize vitamin C and must acquire it through diet for survival. In addition to uncovering several metabolic changes in vitamin C deficient zebrafish previously reported in genetic animal models of vitamin C deficiency, we found evidence for increased purine nucleotide cycle activity. These results demonstrate the suitability of zebrafish for the study of dietary vitamin C deficiency and highlight the roles of vitamin C in energy metabolism. The third chapter describes a metabolomics driven effort to characterize the antiobesity effects and mechanisms of xanthohumol, a prenylated flavonoid found in hops. Based on a metabolomics analysis of plasma from fatty rats treated with xanthohumol, we measure the bioenergetic effects of xanthohumol on cells in culture and find that it is a general mitochondrial uncoupler. We hypothesize that it is through this mechanism that xanthohumol exerts its anti-obesity effects in vivo. The fourth chapter investigates the temporal metabolome changes that occur during adipocyte differentiation. Using time course metabolomics, we uncovered increases in several uncharacterized di- and tripeptides, presumably products of protein degradation. We then treated differentiating adipocytes with 18O labeled water and found incorporation of 18O into the peptides, confirming them as products of peptide or protein hydrolysis. In addition, H218O metabolomics revealed enhanced flux through the CDP-choline cycle and activation of glutaminolysis during adipocyte differentiation, highlighting the utility of 18O labeled water metabolomics to uncover alterations in metabolic pathways undetectable with a typical metabolomics experiment.

Plant Metabolomics

Author : Kazuki Saito
Publisher : Springer Science & Business Media
ISBN 13 : 3540297820
Total Pages : 351 pages
Book Rating : 4.5/5 (42 download)

DOWNLOAD NOW!

Book Synopsis Plant Metabolomics by : Kazuki Saito

Download or read book Plant Metabolomics written by Kazuki Saito and published by Springer Science & Business Media. This book was released on 2006-06-29 with total page 351 pages. Available in PDF, EPUB and Kindle. Book excerpt: Metabolomics – which deals with all metabolites of an organism – is a rapidly-emerging sector of post-genome research fields. It plays significant roles in a variety of fields from medicine to agriculture and holds a fundamental position in functional genomics studies and their application in plant biotechnology. This volume comprehensively covers plant metabolomics for the first time. The chapters offer cutting-edge information on analytical technology, bioinformatics and applications. They were all written by leading researchers who have been directly involved in plant metabolomics research throughout the world. Up-to-date information and future developments are described, thereby producing a volume which is a landmark of plant metabolomics research and a beneficial guideline to graduate students and researchers in academia, industry, and technology transfer organizations in all plant science fields.

Development and Application of Mass Spectrometry-based Metabolomics for Characterization of Type 2 Diabetes and Diabetic Cardiomyopathy

Download Development and Application of Mass Spectrometry-based Metabolomics for Characterization of Type 2 Diabetes and Diabetic Cardiomyopathy PDF Online Free

Author : Benjamin Wancewicz
Publisher :
ISBN 13 :
Total Pages : 0 pages
Book Rating : 4.:/5 (145 download)

DOWNLOAD NOW!

Book Synopsis Development and Application of Mass Spectrometry-based Metabolomics for Characterization of Type 2 Diabetes and Diabetic Cardiomyopathy by : Benjamin Wancewicz

Download or read book Development and Application of Mass Spectrometry-based Metabolomics for Characterization of Type 2 Diabetes and Diabetic Cardiomyopathy written by Benjamin Wancewicz and published by . This book was released on 2024 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Diabetes mellitus (DM) is a global epidemic, currently affecting more than 537 million adults worldwide. Type 2 diabetes mellitus (T2D) is the predominant form of DM, making up over 90% of the cases. Individuals with diabetes are at an increased risk of cardiovascular complications, which is termed diabetic cardiomyopathy (DCM), and these complications are their primary source of mortality. The early stages of DCM are marked by shifts in glucose metabolism, as well as lipid metabolism. Mass-spectrometry metabolomics has been used to study these shifts and is a natural tool to survey the large changes that are present. However, challenges remain in accessing the plasma and cardiac metabolome in a comprehensive manner. The research described herein involves the development and application of metabolomics approaches, namely in sample preparation and characterization by mass spectrometry, to investigate metabolic shifts in T2D. Chapter 1 provides an introduction to diabetes, metabolomics, and the mouse models used to study diabetes. In Chapter 2, a highly reproducible and high-throughput method for plasma metabolomics was developed. Using a single-phase extraction method, flow injection, and the Fourier-transform ion cyclotron resonance mass spectrometer (FTICR MS), a broad range of metabolites and lipids were observed. In Chapter 3, this metabolomics platform was used to analyze plasma samples from patients with diabetes undergoing either metformin or diet and lifestyle intervention. Fatty acid metabolism was observed to differ between the two diabetic groups with the levels in the metformin group being significantly different than diet and lifestyle group. In Chapter 4, a comprehensive analysis of the human heart was undertaken with six extraction methods and four metabolomics platforms to determine the best approach for investigating cardiac metabolism. In Chapter 5, the heart of a high fat diet mouse model of DCM was investigated. Alterations in the lipidome were observed with a particular shift in acyl chain composition in ceramides, triglycerides, and cardiolipins.

Mass Spectrometry-based Metabolomics and Lipidomics Empowered by Software Enables Hardware and Applications

Author : Benton J. Anderson
Publisher :
ISBN 13 :
Total Pages : 0 pages
Book Rating : 4.:/5 (143 download)

DOWNLOAD NOW!

Book Synopsis Mass Spectrometry-based Metabolomics and Lipidomics Empowered by Software Enables Hardware and Applications by : Benton J. Anderson

Download or read book Mass Spectrometry-based Metabolomics and Lipidomics Empowered by Software Enables Hardware and Applications written by Benton J. Anderson and published by . This book was released on 2023 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: This work describes advancements in mass spectrometry metabolomics and lipidomics. Chapter 1 introduces mass spectrometry omics for biological discovery and puts the field in the context of the broader omics field, and gives a framework for how this author thinks about mass spectrometry as a collaborative entity for describing and enabling biological insights across fields and groups of people. Chapter 2 describes the Nile rat plasma lipidomics and polar metabolomics in support of developing the animal as a complementary model of human diabetes. Chapter 3 describes advancements in lipidomics data processing in LipiDex 2, focusing on quality control in Degreaser QC module and MSn tree-based fragmentation method support via library generation and integrated support in spectral matching and peak annotation modules. Chapter 4 details the development of a real-time mass spectrometry-based urine metabolomics platform. Chapter 5 concludes the work with final thoughts on the role of the mass spectrometry analyst, future directions for lipidomics and future software upgrades to the LipiDex 2 feature set.

Mass Spectrometry-Based Metabolomics: Platform Development and Application to Neurodegenerative Disease

Author : He Huang
Publisher :
ISBN 13 :
Total Pages : 236 pages
Book Rating : 4.:/5 (114 download)

DOWNLOAD NOW!

Book Synopsis Mass Spectrometry-Based Metabolomics: Platform Development and Application to Neurodegenerative Disease by : He Huang

Download or read book Mass Spectrometry-Based Metabolomics: Platform Development and Application to Neurodegenerative Disease written by He Huang and published by . This book was released on 2017 with total page 236 pages. Available in PDF, EPUB and Kindle. Book excerpt: Metabolites are small molecules that participate in critical cellular processes that include energy production, macromolecules biosynthesis, and signaling pathways. Metabolic dysregulation is associated with changes in homeostasis and disease pathology. Measuring metabolite changes at the sites of tissue dysfunction can reveal new mechanisms that could serve as therapeutic targets and aid in the biomarker discovery. In this dissertation, we develop an untargeted metabolomic platform based on tandem mass spectrometry (TripleTOF 5600+, SCIEX). The platform consists of: hydrophilic interaction chromatography - mass spectrometry (HILIC-MS) to separate and detect polar metabolites and direct infusion-based based shotgun lipidomics. The platform allows global metabolic profiling in approximately thirty minutes. Three neurodegenerative diseases, including multiple sclerosis, normal pressure hydrocephalus (NPH), and Chiari malformation type I (CMI) are examined by this platform. First, dimethyl fumarate (DMF) treated oligodendrocytes are examined to provide mechanistic insight into potential neuroprotective pathways that may be upregulated by this treatment. Results indicate DMF alters levels of intermediates in the citric acid cycle to induce antioxidant responses in oligodendrocytes. Secondly, cerebrospinal fluid (CSF) samples were collected longitudinally from NPH patients following moderate exercise in order to elucidate pathological mechanisms of this disease as well as categorize potential biomarkers of treatment response. The results indicate that moderate exercise induces changes in CSF metabolites in NPH patient. These metabolic alterations are associated with the levels of vascular endothelial growth factor (VEGF) and patient clinical responses to shunting. Finally, both CSF and serum metabolites of CMI are investigated. Neurotransmitter and neuropeptide alterations were found to be associated with CMI. These results suggest that alterations in neurotransmission might underlie symptoms such as pain in this disorder. In this dissertation, unique metabolic alterations are detected by the untargeted metabolomic platform and suggest novel pathways that might be further examined as therapeutic interventions to alleviate neurological symptoms and promote CNS regeneration.

Mass Spectrometry-Based Metabolomics

Author : Sastia Prama Putri
Publisher : CRC Press
ISBN 13 : 1482223775
Total Pages : 284 pages
Book Rating : 4.4/5 (822 download)

DOWNLOAD NOW!

Book Synopsis Mass Spectrometry-Based Metabolomics by : Sastia Prama Putri

Download or read book Mass Spectrometry-Based Metabolomics written by Sastia Prama Putri and published by CRC Press. This book was released on 2016-04-21 with total page 284 pages. Available in PDF, EPUB and Kindle. Book excerpt: Mass Spectrometry-Based Metabolomics: A Practical Guide is a simple, step-by-step reference for profiling metabolites in a target organism. It discusses optimization of sample preparation for urine, serum, blood, tissue, food, and plant and animal cell samples. Encompassing three different technical fields-biology, analytical chemistry, and informa

Proteomic and Metabolomic Approaches to Biomarker Discovery

Author : Haleem J. Issaq
Publisher : Academic Press
ISBN 13 : 0123947952
Total Pages : 489 pages
Book Rating : 4.1/5 (239 download)

DOWNLOAD NOW!

Book Synopsis Proteomic and Metabolomic Approaches to Biomarker Discovery by : Haleem J. Issaq

Download or read book Proteomic and Metabolomic Approaches to Biomarker Discovery written by Haleem J. Issaq and published by Academic Press. This book was released on 2013-05-20 with total page 489 pages. Available in PDF, EPUB and Kindle. Book excerpt: Proteomic and Metabolomic Approaches to Biomarker Discovery demonstrates how to leverage biomarkers to improve accuracy and reduce errors in research. Disease biomarker discovery is one of the most vibrant and important areas of research today, as the identification of reliable biomarkers has an enormous impact on disease diagnosis, selection of treatment regimens, and therapeutic monitoring. Various techniques are used in the biomarker discovery process, including techniques used in proteomics, the study of the proteins that make up an organism, and metabolomics, the study of chemical fingerprints created from cellular processes. Proteomic and Metabolomic Approaches to Biomarker Discovery is the only publication that covers techniques from both proteomics and metabolomics and includes all steps involved in biomarker discovery, from study design to study execution. The book describes methods, and presents a standard operating procedure for sample selection, preparation, and storage, as well as data analysis and modeling. This new standard effectively eliminates the differing methodologies used in studies and creates a unified approach. Readers will learn the advantages and disadvantages of the various techniques discussed, as well as potential difficulties inherent to all steps in the biomarker discovery process. A vital resource for biochemists, biologists, analytical chemists, bioanalytical chemists, clinical and medical technicians, researchers in pharmaceuticals, and graduate students, Proteomic and Metabolomic Approaches to Biomarker Discovery provides the information needed to reduce clinical error in the execution of research. - Describes the use of biomarkers to reduce clinical errors in research - Includes techniques from a range of biomarker discoveries - Covers all steps involved in biomarker discovery, from study design to study execution

Advancing Mass Spectrometry-based Metabolomics and Proteomics

Author : Ling Hao
Publisher :
ISBN 13 :
Total Pages : 0 pages
Book Rating : 4.:/5 (112 download)

DOWNLOAD NOW!

Book Synopsis Advancing Mass Spectrometry-based Metabolomics and Proteomics by : Ling Hao

Download or read book Advancing Mass Spectrometry-based Metabolomics and Proteomics written by Ling Hao and published by . This book was released on 2017 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Quantitative analysis of endogenous biomolecules offers a wealth of clues to understand cell biology and disease mechanisms. Mass spectrometry (MS) has become a foremost technology for the study of biomolecules and their dynamic alterations involved in disease processes. This dissertation is devoted to the development of novel and improved MS-based metabolomic and proteomic methodologies and the application to human disease studies. Specifically, both label-free and stable isotope labeling strategies have been developed for the analysis of metabolites, peptides, and proteins in various biological systems. Label-free methods provide straightforward measurements of biomolecules and are combined with advanced bioinformatics tools for biomarker discovery. Stable isotope labeling, particularly custom DiLeu, iDiLeu, and mdDiLeu tags, offers high-throughput and accurate quantification of biomolecules. Novel online software platform has also been developed for automated omics data analysis. The advancements of analytical methodologies in this work have been mainly achieved on standard flow liquid chromatography (LC)-MS, nanoflow LC-MS, and capillary electrophoresis (CE)-MS platforms. Comprehensive metabolomic and proteomic studies have been successfully applied to disease biomarker discovery for human Preclinical Alzheimer's Disease and Lower Urinary Tract Symptoms in human and mouse models.

Development of Mass Spectrometry-based Bioanalytical Platforms for Metabolomics

Author : Katyeny Manuela da Silva
Publisher :
ISBN 13 :
Total Pages : 0 pages
Book Rating : 4.:/5 (139 download)

DOWNLOAD NOW!

Book Synopsis Development of Mass Spectrometry-based Bioanalytical Platforms for Metabolomics by : Katyeny Manuela da Silva

Download or read book Development of Mass Spectrometry-based Bioanalytical Platforms for Metabolomics written by Katyeny Manuela da Silva and published by . This book was released on 2023 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Mass Spectrometry-Based Metabolomics in Clinical and Herbal Medicines

Author : Aihua Zhang
Publisher : John Wiley & Sons
ISBN 13 : 3527835741
Total Pages : 292 pages
Book Rating : 4.5/5 (278 download)

DOWNLOAD NOW!

Book Synopsis Mass Spectrometry-Based Metabolomics in Clinical and Herbal Medicines by : Aihua Zhang

Download or read book Mass Spectrometry-Based Metabolomics in Clinical and Herbal Medicines written by Aihua Zhang and published by John Wiley & Sons. This book was released on 2021-08-20 with total page 292 pages. Available in PDF, EPUB and Kindle. Book excerpt: Highlights the importance and benefit of mass spectrometry-based metabolomics for identifying biomarkers that accurately screen for potential biomarkers of diseases Mass spectrometry-based metabolomics offer new opportunities for biomarker discovery in complex diseases and may provide pathological understanding of diseases beyond traditional technologies. It is the systematic analysis of low-molecular-weight metabolites in biological samples and has been applied to discovering and identifying the perturbed pathways. Currently, mass spectrometry-based metabolomics has become an important tool in clinical research and the diagnosis of human disease. Mass Spectrometry-Based Metabolomics in Clinical and Herbal Medicines comprehensively presents the current state, challenges, and applications of high-throughput mass spectrometry-based metabolomics such as metabolites analysis, biomarker discovery, technical challenges, discovery of natural product, mechanism interpretation of action, discovery of active ingredients, clinical application and precision medicine, and enhancing their biomedical value in a real world of biomedicine, shedding light on the potential for spectrometry-based metabolomics. It highlights the value of mass spectrometry-based metabolomics and metabolism to address the complexity of herbal medicines in systems pharmacology, especially, to link phytochemical analysis with the assessment of pharmacological effect and therapeutic potential. Each chapter has been laid out with introduction, method, up-to-date literature, identification of biomarker, and applications Covers the current state, challenges, and applications of high-throughput mass spectrometry-based metabolomics in the discovery of biomarker, active ingredients, natural product, etc. Constitutes a unique and indispensable practical guide for any phytochemistry or related laboratory, and provides hands-on description of new techniques Provides a guide for new practitioners of pharmacologists, pharmacological scholars, drug developers, botanist, researchers of traditional medicines. Mass Spectrometry-Based Metabolomics in Clinical and Herbal Medicines provides a landmark of mass spectrometry-based metabolomics research and a beneficial guideline to graduate students and researchers in academia, industry, and technology transfer organizations in all biomedical science fields.

A Mass Spectrometry Imaging-based Platform

Author :
Publisher :
ISBN 13 :
Total Pages : 0 pages
Book Rating : 4.:/5 (861 download)

DOWNLOAD NOW!

Book Synopsis A Mass Spectrometry Imaging-based Platform by :

Download or read book A Mass Spectrometry Imaging-based Platform written by and published by . This book was released on 2013 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Mass spectrometry imaging (MSI) has evolved as a promising technology to map a broad range of molecules ranging from metabolites, peptides to proteins in an anatomical context. In this work, a multi-dimensional MSI-based platform was developed and applied to investigate several intriguing biological problems. This dissertation first introduces the rationale and recent progress of MSI, reviews its emerging applications to various fields and outlines one limitation that the detection of low molecular weight (MW) species is usually hindered by matrix-derived peaks. An ionless matrix referred as the proton sponge is therefore investigated as a solution to eliminate the interferences. It allows for probing metabolites in roots and root nodules of leguminous plants Medicago truncatula that possess symbiotic interactions with nitrogen-fixing rhizobia, highlighting differences in metabolic distribution between roots and nodules. In addition to exploring novel matrices, high resolution and high accuracy MSI (HRMSI) is also developed to circumvent matrix interferences by resolving endogenous analytes from background peaks. Novel spatial information of multiple metabolites and neurotransmitters is gleaned using rodent and crustacean brain specimens in this proof-of-principle study. Beyond metabolites, the HRMSI-based approach is applied to map neuropeptides in crustacean stomatogastric ganglia. Complementary to MALDI-MS, electrospray ionization (ESI)-MS-based experiments deliver another suite of neuropeptides. We therefore combine multiple MS instruments and compared their performances for characterization of novel neuropeptides from a marine model organism, the spiny lobster Panulirus interruptus. In addition to peptides, MALDI-MSI has also been applied to mapping protein distributions in the brain under pharmacological treatment. Specifically, alterations of protein expression patterns in the brains of infant rats were studied following neonatal exposure to the N-methyl-D-aspartate receptor antagonist dizocilpine (MK 801). A strategy combining profiling and imaging MS and top-down high-resolution tandem MS is utilized to discover putative protein biomarkers that may serve as potential targets of treatments for neurodevelopmental disorders. This work not only improves upon MSI by developing new methods, but also presents a useful platform that integrates MALDI-MSI with ESI-MS in exploring the underlying chemistry of various biological models exemplified by rodent, crustacean and plant-bacteria systems, and demonstrates wide applicability to future research.

Advanced Mass Spectrometry-based Analytical Separation Techniques for Probing the Polar Metabolome

Author : Rawi Ramautar
Publisher : Royal Society of Chemistry
ISBN 13 : 1839163534
Total Pages : 277 pages
Book Rating : 4.8/5 (391 download)

DOWNLOAD NOW!

Book Synopsis Advanced Mass Spectrometry-based Analytical Separation Techniques for Probing the Polar Metabolome by : Rawi Ramautar

Download or read book Advanced Mass Spectrometry-based Analytical Separation Techniques for Probing the Polar Metabolome written by Rawi Ramautar and published by Royal Society of Chemistry. This book was released on 2021-07-13 with total page 277 pages. Available in PDF, EPUB and Kindle. Book excerpt: The efficient analysis of polar and charged metabolites in biological samples remains a huge challenge in the field of metabolomics. Over the past years, novel mass spectrometry-based analytical tools have been developed to enable the sensitive and efficient profiling of polar ionogenic metabolites in various biological samples. This book gives the reader a comprehensive overview of these recent technological developments. Topics covered include the use of chemical labelling strategies for allowing the analysis of polar metabolites using reversed-phase liquid chromatography–mass spectrometry (RPLC-MS) and the latest methodological developments in RPLC-MS, hydrophilic interaction liquid chromatography (HILIC)-MS and ion-pair LC-MS approaches. Attention is also paid to developments in nano-LC-MS and capillary electrophoresis–mass spectrometry methods specifically for profiling polar metabolites in small volume biological samples. The utility of ion-mobility MS and NMR spectroscopy will also be outlined. Sample preparation is the key part in the analytical workflow employed for metabolomics. Therefore, ample emphasis will be given on recent solid-phase extraction and solid-phase micro-extraction methods. Finally, analytical techniques for chiral metabolic profiling will also be considered. Discussing the state-of-the-art of the proposed topics in one single book for probing the polar metabolome, using relevant examples, is unique and needed in the metabolomics field. This book has relevance and appeal to an international audience of analytical and biomedical researchers in industry and academia.

Metabolomics in Crop Research – Current and Emerging Methodologies

Author : Marta Sousa Silva
Publisher : Frontiers Media SA
ISBN 13 : 2889631583
Total Pages : 181 pages
Book Rating : 4.8/5 (896 download)

DOWNLOAD NOW!

Book Synopsis Metabolomics in Crop Research – Current and Emerging Methodologies by : Marta Sousa Silva

Download or read book Metabolomics in Crop Research – Current and Emerging Methodologies written by Marta Sousa Silva and published by Frontiers Media SA. This book was released on 2019-11-18 with total page 181 pages. Available in PDF, EPUB and Kindle. Book excerpt: The plant metabolome is highly complex, being composed of over 200,000 metabolites. The characterization of these small molecules has been crucial to study plant growth and development as well as their response to environmental changes. The potential of metabolomics in plant research, particularly if applied to crop plants, is also extremely valuable in the discovery of biomarkers and in the improvement of crop yield and quality. This Frontiers Research Topic addresses many applications of metabolomics to crop research, based on different analytical platforms, including mass spectrometry, and nuclear magnetic resonance. It comprises 13 articles from 109 authors that show the importance and the contribution of metabolomics in the analysis of crop’s traceability and genetic variation, in the study of fruit development, and in the understanding of the plant’s response to the environment and to different biotic and abiotic stresses.

Method Development and Application of Mass Spectrometry Imaging to Study Symbiotic Relationships Between Bacteria and Host Organisms

Download Method Development and Application of Mass Spectrometry Imaging to Study Symbiotic Relationships Between Bacteria and Host Organisms PDF Online Free

Author :
Publisher :
ISBN 13 :
Total Pages : 0 pages
Book Rating : 4.:/5 (949 download)

DOWNLOAD NOW!

Book Synopsis Method Development and Application of Mass Spectrometry Imaging to Study Symbiotic Relationships Between Bacteria and Host Organisms by :

Download or read book Method Development and Application of Mass Spectrometry Imaging to Study Symbiotic Relationships Between Bacteria and Host Organisms written by and published by . This book was released on 2016 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Metabolic profiling can help shed light on cellular mechanisms; however a major technical challenge is to study endogenous metabolomic pathways without perturbing them. Most of the techniques currently in use for metabolomics studies in biological systems rely on tissue extracts which destroy the samples and result in the loss of information about analyte distribution within the tissue. Mass spectrometry imaging (MSI) has evolved as a promising technology to map a wide range of biomolecules in an anatomical context. In this dissertation, a multi-dimensional mass spectrometry-based platform was developed and applied to study interactions between bacteria and host organisms. Specifically, this work aimed to study the symbiotic relationship between soil bacteria and legume plants for agricultural and environmental sustainability applications, as well as the symbiosis between bacteria and leaf cutter ants and humans and our gut microbiota for the discovery of potentially novel antibiotics and antifungals. High-resolution, accurate-mass (HRAM) matrix-assisted laser desorption/ ionization (MALDI)-MSI was used to compare differentially treated biological samples, and liquid chromatography (LC)- electrospray ionization (ESI)- tandem mass spectrometry (MS/MS) coupled with database searching was used as a complementary mass spectrometry technique to identify interesting metabolites chosen from the MSI experiments. This work not only improves upon MSI by exploring novel sample preparation methods, but also presents a useful platform that integrates MALDI-MSI with ESI-MS in exploring the underlying chemistry of several biological symbiotic systems.

Processing Metabolomics and Proteomics Data with Open Software

Author : Robert Winkler
Publisher : Royal Society of Chemistry
ISBN 13 : 1788017218
Total Pages : 460 pages
Book Rating : 4.7/5 (88 download)

DOWNLOAD NOW!

Book Synopsis Processing Metabolomics and Proteomics Data with Open Software by : Robert Winkler

Download or read book Processing Metabolomics and Proteomics Data with Open Software written by Robert Winkler and published by Royal Society of Chemistry. This book was released on 2020-03-19 with total page 460 pages. Available in PDF, EPUB and Kindle. Book excerpt: Metabolomics and proteomics allow deep insights into the chemistry and physiology of biological systems. This book expounds open-source programs, platforms and programming tools for analysing metabolomics and proteomics mass spectrometry data. In contrast to commercial software, open-source software is created by the academic community, which facilitates the direct interaction between users and developers and accelerates the implementation of new concepts and ideas. The first section of the book covers the basics of mass spectrometry, experimental strategies, data operations, the open-source philosophy, metabolomics, proteomics and statistics/ data mining. In the second section, active programmers and users describe available software packages. Included tutorials, datasets and code examples can be used for training and for building custom workflows. Finally, every reader is invited to participate in the open science movement.

Mass Spectrometry in Metabolomics

Author : Daniel Raftery
Publisher : Humana Press
ISBN 13 : 9781493912599
Total Pages : 360 pages
Book Rating : 4.9/5 (125 download)

DOWNLOAD NOW!

Book Synopsis Mass Spectrometry in Metabolomics by : Daniel Raftery

Download or read book Mass Spectrometry in Metabolomics written by Daniel Raftery and published by Humana Press. This book was released on 2014-10-01 with total page 360 pages. Available in PDF, EPUB and Kindle. Book excerpt: Metabolomics is a fast growing field in systems biology and offers a powerful and promising approach for a large range of applications. Metabolomics focuses on deriving the concentrations and fluxes of low molecular weight metabolites in bio-fluids, cells or tissue, plants, foods and related samples and this information provides enormous detail on biological systems and their current status. Mass Spectrometry in Metabolomics: Methods and Protocols presents a broad coverage of the major mass spectrometry (MS)-based metabolomics methods and applications. MS is one of most powerful and commonly used analytical methods in metabolomics; because so many different MS systems are used in metabolomics, this volume includes a wide variety such as triple quads, time of flight, Fourier transform ion cyclotron resonance and even simple quadrupole systems. A wide range of studies are described, with samples ranging from blood and urine to tissue and even plants. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, Mass Spectrometry in Metabolomics: Methods and Protocols seeks to serve both professionals and novices with its well-honed methodologies in an effort to further the dynamic field of metabolomics.

Development and Application of Mass Spectrometry-based Metabolomics Methods for Disease Biomarker Identification

Author : Lily Victoria Tong
Publisher :
ISBN 13 :
Total Pages : 299 pages
Book Rating : 4.:/5 (367 download)

DOWNLOAD NOW!

Book Synopsis Development and Application of Mass Spectrometry-based Metabolomics Methods for Disease Biomarker Identification by : Lily Victoria Tong

Download or read book Development and Application of Mass Spectrometry-based Metabolomics Methods for Disease Biomarker Identification written by Lily Victoria Tong and published by . This book was released on 2008 with total page 299 pages. Available in PDF, EPUB and Kindle. Book excerpt: (cont.) We sought to investigate whether the addition of metabolomic information would result in increased accuracy of mortality prediction. One hundred twenty patient samples were obtained from a national dialysis study (equally representing death and survival within 90 days of starting dialysis) and analyzed according to our protocol. Two feature selection algorithms were applied to identify significant metabolites distinguishing death and survival, and the corresponding models resulted in improved receiver-operating characteristic (ROC) curve areas of 0.85 and 0.93. This result constitutes a significant improvement from existing clinical models, which at best result in ROC curve areas of 0.80. Based on this work, we hypothesize that our observed differential fatty acid concentrations are indicative of impaired fatty acid oxidation, leading to insulin resistance in ESRD patients (regardless of Type II diabetes status) and eventually, patient mortality.