Confocal And Two Photon Microscopy

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Confocal and Two-Photon Microscopy

Author : Alberto Diaspro
Publisher : John Wiley & Sons
ISBN 13 : 0471409200
Total Pages : 600 pages
Book Rating : 4.4/5 (714 download)

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Book Synopsis Confocal and Two-Photon Microscopy by : Alberto Diaspro

Download or read book Confocal and Two-Photon Microscopy written by Alberto Diaspro and published by John Wiley & Sons. This book was released on 2001-11-22 with total page 600 pages. Available in PDF, EPUB and Kindle. Book excerpt: Confocal and Two-Photon Microscopy Foundations, Applications, and Advances Edited by Alberto Diaspro Confocal and two-photon fluorescence microscopy has provided researchers with unique possibilities of three-dimensional imaging of biological cells and tissues and of other structures such as semiconductor integrated circuits. Confocal and Two-Photon Microscopy: Foundations, Applications, and Advances provides clear, comprehensive coverage of basic foundations, modern applications, and groundbreaking new research developments made in this important area of microscopy. Opening with a foreword by G. J. Brakenhoff, this reference gathers the work of an international group of renowned experts in chapters that are logically divided into balanced sections covering theory, techniques, applications, and advances, featuring: * In-depth discussion of applications for biology, medicine, physics, engineering, and chemistry, including industrial applications * Guidance on new and emerging imaging technology, developmental trends, and fluorescent molecules * Uniform organization and review-style presentation of chapters, with an introduction, historical overview, methodology, practical tips, applications, future directions, chapter summary, and bibliographical references * Companion FTP site with full-color photographs * The significant experience of pioneers, leaders, and emerging scientists in the field of confocal and two-photon excitation microscopy Confocal and Two-Photon Microscopy: Foundations, Applications, and Advances is invaluable to researchers in the biological sciences, tissue and cellular engineering, biophysics, bioengineering, physics of matter, and medicine, who use these techniques or are involved in developing new commercial instruments.

Microscopy Techniques

Author : Jens Rietdorf
Publisher : Springer Science & Business Media
ISBN 13 : 9783540236986
Total Pages : 344 pages
Book Rating : 4.2/5 (369 download)

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Book Synopsis Microscopy Techniques by : Jens Rietdorf

Download or read book Microscopy Techniques written by Jens Rietdorf and published by Springer Science & Business Media. This book was released on 2005-06-23 with total page 344 pages. Available in PDF, EPUB and Kindle. Book excerpt: With contributions by numerous experts

Confocal Microscopy and Multiphoton Excitation Microscopy

Author : Barry R. Masters
Publisher : SPIE Press
ISBN 13 : 9780819461186
Total Pages : 236 pages
Book Rating : 4.4/5 (611 download)

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Book Synopsis Confocal Microscopy and Multiphoton Excitation Microscopy by : Barry R. Masters

Download or read book Confocal Microscopy and Multiphoton Excitation Microscopy written by Barry R. Masters and published by SPIE Press. This book was released on 2006 with total page 236 pages. Available in PDF, EPUB and Kindle. Book excerpt: This text guides you through the principles and practical techniques of confocal and multiphoton microscopy. It also describes the historical connections and parallel inventions that resulted in modern techniques of live cell imaging and their use in biology and medicine. You will find comparisons of different types of confocal and multiphoton microscopes, solutions to the problems one would encounter when using various microscopic techniques, tips on selecting equipment, and an extensive annotated bibliography of additional resources.

The Resolution and Contrast of Confocal and Two-photon Laser Scanning Fluorescence Microscopy in Turbid Media

Author : Yuin-Jen D. Hsu
Publisher :
ISBN 13 :
Total Pages : 142 pages
Book Rating : 4.E/5 ( download)

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Book Synopsis The Resolution and Contrast of Confocal and Two-photon Laser Scanning Fluorescence Microscopy in Turbid Media by : Yuin-Jen D. Hsu

Download or read book The Resolution and Contrast of Confocal and Two-photon Laser Scanning Fluorescence Microscopy in Turbid Media written by Yuin-Jen D. Hsu and published by . This book was released on 1996 with total page 142 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Selected Papers on Multiphoton Excitation Microscopy

Author : Barry R. Masters
Publisher : SPIE-International Society for Optical Engineering
ISBN 13 :
Total Pages : 706 pages
Book Rating : 4.:/5 (318 download)

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Book Synopsis Selected Papers on Multiphoton Excitation Microscopy by : Barry R. Masters

Download or read book Selected Papers on Multiphoton Excitation Microscopy written by Barry R. Masters and published by SPIE-International Society for Optical Engineering. This book was released on 2003 with total page 706 pages. Available in PDF, EPUB and Kindle. Book excerpt: In the last decade multiphoton excitation microscopy has emerged as an important technique with ever increasing numbers of significant applications in the fields of biology, chemistry, physics, and medicine. This volume contains key papers on the following topics: developments of nonlinear optical spectroscopy and nonlinear scanning microscopy (SHG, CARS); theory and techniques of multiphoton excitation microscopy; development of laser sources; single-molecule studies; and applications to biology, cell biology, embryology and developmental biology, neuroscience, dermatology, and optical biopsy. A comprehensive bibliography follows the reprinted papers.

High Resolution Imaging in Microscopy and Ophthalmology

Author : Josef F. Bille
Publisher : Springer
ISBN 13 : 3030166384
Total Pages : 407 pages
Book Rating : 4.0/5 (31 download)

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Book Synopsis High Resolution Imaging in Microscopy and Ophthalmology by : Josef F. Bille

Download or read book High Resolution Imaging in Microscopy and Ophthalmology written by Josef F. Bille and published by Springer. This book was released on 2019-08-13 with total page 407 pages. Available in PDF, EPUB and Kindle. Book excerpt: This open access book provides a comprehensive overview of the application of the newest laser and microscope/ophthalmoscope technology in the field of high resolution imaging in microscopy and ophthalmology. Starting by describing High-Resolution 3D Light Microscopy with STED and RESOLFT, the book goes on to cover retinal and anterior segment imaging and image-guided treatment and also discusses the development of adaptive optics in vision science and ophthalmology. Using an interdisciplinary approach, the reader will learn about the latest developments and most up to date technology in the field and how these translate to a medical setting. High Resolution Imaging in Microscopy and Ophthalmology – New Frontiers in Biomedical Optics has been written by leading experts in the field and offers insights on engineering, biology, and medicine, thus being a valuable addition for scientists, engineers, and clinicians with technical and medical interest who would like to understand the equipment, the applications and the medical/biological background. Lastly, this book is dedicated to the memory of Dr. Gerhard Zinser, co-founder of Heidelberg Engineering GmbH, a scientist, a husband, a brother, a colleague, and a friend.

Spectroscopy for Materials Characterization

Author : Simonpietro Agnello
Publisher : John Wiley & Sons
ISBN 13 : 1119697328
Total Pages : 500 pages
Book Rating : 4.1/5 (196 download)

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Book Synopsis Spectroscopy for Materials Characterization by : Simonpietro Agnello

Download or read book Spectroscopy for Materials Characterization written by Simonpietro Agnello and published by John Wiley & Sons. This book was released on 2021-09-08 with total page 500 pages. Available in PDF, EPUB and Kindle. Book excerpt: SPECTROSCOPY FOR MATERIALS CHARACTERIZATION Learn foundational and advanced spectroscopy techniques from leading researchers in physics, chemistry, surface science, and nanoscience In Spectroscopy for Materials Characterization, accomplished researcher Simonpietro Agnello delivers a practical and accessible compilation of various spectroscopy techniques taught and used to today. The book offers a wide-ranging approach taught by leading researchers working in physics, chemistry, surface science, and nanoscience. It is ideal for both new students and advanced researchers studying and working with spectroscopy. Topics such as confocal and two photon spectroscopy, as well as infrared absorption and Raman and micro-Raman spectroscopy, are discussed, as are thermally stimulated luminescence and spectroscopic studies of radiation effects on optical materials. Each chapter includes a basic introduction to the theory necessary to understand a specific technique, details about the characteristic instrumental features and apparatuses used, including tips for the appropriate arrangement of a typical experiment, and a reproducible case study that shows the discussed techniques used in a real laboratory. Readers will benefit from the inclusion of: Complete and practical case studies at the conclusion of each chapter to highlight the concepts and techniques discussed in the material Citations of additional resources ideal for further study A thorough introduction to the basic aspects of radiation matter interaction in the visible-ultraviolet range and the fundamentals of absorption and emission A rigorous exploration of time resolved spectroscopy at the nanosecond and femtosecond intervals Perfect for Master and Ph.D. students and researchers in physics, chemistry, engineering, and biology, Spectroscopy for Materials Characterization will also earn a place in the libraries of materials science researchers and students seeking a one-stop reference to basic and advanced spectroscopy techniques.

Confocal and Two-photon Microscopy

Author : Sarah Kefayati
Publisher :
ISBN 13 :
Total Pages : pages
Book Rating : 4.:/5 (13 download)

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Book Synopsis Confocal and Two-photon Microscopy by : Sarah Kefayati

Download or read book Confocal and Two-photon Microscopy written by Sarah Kefayati and published by . This book was released on 2009 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:

Introduction to Optical Microscopy

Author : Jerome Mertz
Publisher : Cambridge University Press
ISBN 13 : 1108428304
Total Pages : 475 pages
Book Rating : 4.1/5 (84 download)

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Book Synopsis Introduction to Optical Microscopy by : Jerome Mertz

Download or read book Introduction to Optical Microscopy written by Jerome Mertz and published by Cambridge University Press. This book was released on 2019-08 with total page 475 pages. Available in PDF, EPUB and Kindle. Book excerpt: Presents a fully updated, self-contained textbook covering the core theory and practice of both classical and modern optical microscopy techniques.

The Design, Construction, and Operation of a Two-photon Laser Scanning Confocal Microscope

Author : Benjamin Alexander
Publisher :
ISBN 13 :
Total Pages : 33 pages
Book Rating : 4.:/5 (795 download)

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Book Synopsis The Design, Construction, and Operation of a Two-photon Laser Scanning Confocal Microscope by : Benjamin Alexander

Download or read book The Design, Construction, and Operation of a Two-photon Laser Scanning Confocal Microscope written by Benjamin Alexander and published by . This book was released on 2011 with total page 33 pages. Available in PDF, EPUB and Kindle. Book excerpt: Confocal microscopes have become valuable tools in many fields of study. This work is intended to serve as a primer for basic confocal microscopy theory as well as a description of the design and construction of a homebuilt system in the Elson Lab at the Washington University School of Medicine. A description of the different designs of confocal microscopes is provided, and the capabilities, benefits, and detriments of each is described. The thought process behind each design decision is explained leading to the final system design. A more in depth description of the final design is provided in Chapters Two and Three, but, in short, the final design is a non-descanned, laser scanning, two-photon confocal microscope. The system is designed to integrate as seamlessly as possible with the existing system and to allow for all existing functionality to remain. When completed, the system will be capable of one- and two-photon fluorescence correlation ii spectroscopy (FCS); low-speed, one-photon confocal imaging using a piezo-stage; and high-speed, two-photon confocal imaging using the laser scanning system. Finally, future directions as well as the limitations of the system are also described. All resources necessary to continue work on the system as well as those necessary to use it are provided. This includes: System diagrams, Optical layout plots, Component data sheets, LabVIEW program. These resources are intended to make using, modifying, and improving the system much simpler.

Fluorescence Microscopy

Author : Ulrich Kubitscheck
Publisher : John Wiley & Sons
ISBN 13 : 3527687726
Total Pages : 508 pages
Book Rating : 4.5/5 (276 download)

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Book Synopsis Fluorescence Microscopy by : Ulrich Kubitscheck

Download or read book Fluorescence Microscopy written by Ulrich Kubitscheck and published by John Wiley & Sons. This book was released on 2017-03-27 with total page 508 pages. Available in PDF, EPUB and Kindle. Book excerpt: Zu dem Thema gibt es viele Publikationen, die von Experten für Experten geschrieben wurden. Dieses Buch wendet sich insbesondere an Studenten höherer Semester und Forscher, denen das Hintergrundwissen der Physik fehlt, um neuartige Verfahren der Fluoreszenzmikroskopie zu verstehen. Die zweite Auflage wartet mit neuen Kapiteln und einer erweiterten Einführung auf. Der Schwerpunkt liegt auf der hochauflösenden und Einzelmolekül-Mikroskopie. Jedes Kapitel wurde von einem anerkannten Experten des Fachgebiets geschrieben und sorgfältig überarbeitet, um so die Entwicklungen der letzten Jahre wiederzugeben.

Handbook of Biological Confocal Microscopy

Author : James Pawley
Publisher : Springer Science & Business Media
ISBN 13 : 1475753489
Total Pages : 639 pages
Book Rating : 4.4/5 (757 download)

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Book Synopsis Handbook of Biological Confocal Microscopy by : James Pawley

Download or read book Handbook of Biological Confocal Microscopy written by James Pawley and published by Springer Science & Business Media. This book was released on 2013-04-17 with total page 639 pages. Available in PDF, EPUB and Kindle. Book excerpt: This third edition of a classic text in biological microscopy includes detailed descriptions and in-depth comparisons of parts of the microscope itself, digital aspects of data acquisition and properties of fluorescent dyes, the techniques of 3D specimen preparation and the fundamental limitations, and practical complexities of quantitative confocal fluorescence imaging. Coverage includes practical multiphoton, photodamage and phototoxicity, 3D FRET, 3D microscopy correlated with micro-MNR, CARS, second and third harmonic signals, ion imaging in 3D, scanning RAMAN, plant specimens, practical 3D microscopy and correlated optical tomography.

Optical Imaging in Human Disease and Biological Research

Author : Xunbin Wei
Publisher : Springer Nature
ISBN 13 : 9811576270
Total Pages : 309 pages
Book Rating : 4.8/5 (115 download)

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Book Synopsis Optical Imaging in Human Disease and Biological Research by : Xunbin Wei

Download or read book Optical Imaging in Human Disease and Biological Research written by Xunbin Wei and published by Springer Nature. This book was released on 2021-05-29 with total page 309 pages. Available in PDF, EPUB and Kindle. Book excerpt: The book introduces readers to the basic principle of optical imaging technologies. Focusing on human disease diagnostics using optical imaging methods, it provides essential information for researchers in various fields and discusses the latest trends in optical imaging. In recent decades, there has been a huge increase in imaging technologies and their applications in human diseases diagnostics, including magnetic resonance imaging, x-ray computed tomography, and nuclear tomographic imaging. This book promotes further developments to extend optical imaging to a wider range of disease diagnostics. It is a valuable resource for researchers and students in the field of biomedical optics, as well as for clinicians.

Imaging Neurons

Author : Rafael Yuste
Publisher :
ISBN 13 :
Total Pages : 848 pages
Book Rating : 4.3/5 (91 download)

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Book Synopsis Imaging Neurons by : Rafael Yuste

Download or read book Imaging Neurons written by Rafael Yuste and published by . This book was released on 2000 with total page 848 pages. Available in PDF, EPUB and Kindle. Book excerpt: In the past decade, advances in microscopy have been coupled with new methods of culturing and labeling cells to generate the new science of imaging. Imaging technologies allow investigators to look directly inside living cells and probe their form and function in unprecedented detail. This approach is revolutionizing many aspects of biomedical research, particularly neuroscience, in which visual techniques have traditionally been so important. This manual is the first comprehensive description of the range of imaging technologies being applied to living cells. With its origins in a laboratory course taught at Cold Spring Harbor Laboratory by the editors and contributors, it is packed with the kind of technical detail and practical advice that are essential for success, yet seldom found in the research literature. It covers both established methods and cutting-edge techniques such as multiphoton excitation microscopy and imaging of genetically engineered probes. Although it is neurons to which these technologies are most commonly applied, the methods described are readily adaptable to many other cell types. This book will therefore be an invaluable aid to investigators in cell and developmental biology and immunology as well as neuroscience who wish to take advantage of the extraordinary insights into cellular function offered by imaging technologies.

Principles of Three Dimensional Imaging in Confocal Microscopes

Author : Min Gu
Publisher : World Scientific
ISBN 13 : 9789810225506
Total Pages : 360 pages
Book Rating : 4.2/5 (255 download)

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Book Synopsis Principles of Three Dimensional Imaging in Confocal Microscopes by : Min Gu

Download or read book Principles of Three Dimensional Imaging in Confocal Microscopes written by Min Gu and published by World Scientific. This book was released on 1996 with total page 360 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book discusses the various principles in confocal scanning microscopy which has become a useful tool in many practical fields including biological studies and industrial inspection. The methodology presented in this book is unique and is based on the concept of the three-dimensional transfer functions which have been developed by the author and his colleagues over the last five years. With the 3-D transfer functions, resolving power in 3-D confocal imaging can be defined in a unified way, different optical arrangements can be compared with an insight into their inter-relationship, and images of thick objects can be modeled in terms of the Fourier transform which makes the analysis easy. The aim of this book is to provide a systematic introduction to the concept of the 3-D transfer functions in various confocal microscopes, to describe the methods for the derivation of different 3-D transfer functions, and to explain the principles of 3-D confocal imaging in terms of these functions.

Multiphoton Microscopy

Author : Espen Hartveit
Publisher : Humana
ISBN 13 : 9781493997046
Total Pages : 364 pages
Book Rating : 4.9/5 (97 download)

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Book Synopsis Multiphoton Microscopy by : Espen Hartveit

Download or read book Multiphoton Microscopy written by Espen Hartveit and published by Humana. This book was released on 2020-11-15 with total page 364 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume covers the latest techniques and strategies used in multi-photon excitation (MPE) microscopy. Chapters in this book cover the fundamentals of MPE microscopy as applied to both in vitro and in vivo experimental systems; information on how to combine MPE microscopy with targeted electrophysiological recordings, calcium imaging, and transmembrane voltage imaging; methods to investigate cellular and large-scale neural morphology; signaling in astrocytes; and ways to use MPE microscopy to study the retina. In Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your laboratory. Comprehensive and thorough, Multiphoton Microscopy is a valuable resource for both expert and novice researchers interested in expanding their knowledge and research in this rapidly developing field.

Guide to Research Techniques in Neuroscience

Author : Matt Carter
Publisher : Academic Press
ISBN 13 : 0323915612
Total Pages : 416 pages
Book Rating : 4.3/5 (239 download)

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Book Synopsis Guide to Research Techniques in Neuroscience by : Matt Carter

Download or read book Guide to Research Techniques in Neuroscience written by Matt Carter and published by Academic Press. This book was released on 2022-03-26 with total page 416 pages. Available in PDF, EPUB and Kindle. Book excerpt: Modern neuroscience research is inherently multidisciplinary, with a wide variety of cutting edge new techniques to explore multiple levels of investigation. This Third Edition of Guide to Research Techniques in Neuroscience provides a comprehensive overview of classical and cutting edge methods including their utility, limitations, and how data are presented in the literature. This book can be used as an introduction to neuroscience techniques for anyone new to the field or as a reference for any neuroscientist while reading papers or attending talks. - Nearly 200 updated full-color illustrations to clearly convey the theory and practice of neuroscience methods - Expands on techniques from previous editions and covers many new techniques including in vivo calcium imaging, fiber photometry, RNA-Seq, brain spheroids, CRISPR-Cas9 genome editing, and more - Clear, straightforward explanations of each technique for anyone new to the field - A broad scope of methods, from noninvasive brain imaging in human subjects, to electrophysiology in animal models, to recombinant DNA technology in test tubes, to transfection of neurons in cell culture - Detailed recommendations on where to find protocols and other resources for specific techniques - "Walk-through" boxes that guide readers through experiments step-by-step