Author : National Aeronautics and Space Administration (NASA)
Publisher : Createspace Independent Publishing Platform
ISBN 13 : 9781725576605
Total Pages : 36 pages
Book Rating : 4.5/5 (766 download)
Book Synopsis Comparison of Epifluorescent Viable Bacterial Count Methods by : National Aeronautics and Space Administration (NASA)
Download or read book Comparison of Epifluorescent Viable Bacterial Count Methods written by National Aeronautics and Space Administration (NASA) and published by Createspace Independent Publishing Platform. This book was released on 2018-08-16 with total page 36 pages. Available in PDF, EPUB and Kindle. Book excerpt: Two methods, the 2-(4-Iodophenyl) 3-(4-nitrophenyl) 5-phenyltetrazolium chloride (INT) method and the direct viable count (DVC), were tested and compared for their efficiency for the determination of the viability of bacterial populations. Use of the INT method results in the formation of a dark spot within each respiring cell. The DVC method results in elongation or swelling of growing cells that are rendered incapable of cell division. Although both methods are subjective and can result in false positive results, the DVC method is best suited to analysis of waters in which the number of different types of organisms present in the same sample is assumed to be small, such as processed waters. The advantages and disadvantages of each method are discussed. Rodgers, E. B. and Huff, T. L. Marshall Space Flight Center NASA-TM-103592, NAS 1.15:103592 ...