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Colony Of Clones
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Book Synopsis Hemopoietic Colonies by : D. Metcalf
Download or read book Hemopoietic Colonies written by D. Metcalf and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 397 pages. Available in PDF, EPUB and Kindle. Book excerpt: The cloning of hemopoietic cells in semisolid medium began some 12 years ago, and when colonies of T = and B = lymphocytes were grown by several groups last year, the last major subclasses of hemopoietic cells had been successfully cultured in vitro. The time seemed appropriate to write a short general account of this exciting new area of hematology, emphasizing particularly the potential value of these new techniques for direct studies on patients with leukemia and allied diseases. I had hoped to refer to, or at least record, every paper published in this field, but the literature proved to be larger than I had realized, and furthermore, some areas were so active that chapters had to be rewritten over a period of less than a year. I regret that ·what follows is an incomplete account and no doubt, by the time this book becomes available, the material will be even more out of date. However, I have described the basic techniques in some detail and have tried, where possible, to explain how and why the techniques can fail. It is already possible to see patterns, perhaps even principles, emerging from this work which may well be applicable to other cell populations. These have been emphasized in the text in an attempt to make this review as creative as possible.
Book Synopsis Culture of Animal Cells by : R. Ian Freshney
Download or read book Culture of Animal Cells written by R. Ian Freshney and published by John Wiley & Sons. This book was released on 2015-12-23 with total page 728 pages. Available in PDF, EPUB and Kindle. Book excerpt: Since the publication of the sixth edition of this benchmark text, numerous advances in the field have been made – particularly in stem cells, 3D culture, scale-up, STR profiling, and culture of specialized cells. Culture of Animal Cells: A Manual of Basic Technique and Specialized Applications, Seventh Edition is the updated version of this benchmark text, addressing these recent developments in the field as well as the basic skills and protocols. This eagerly awaited edition reviews the increasing diversity of the applications of cell culture and the proliferation of specialized techniques, and provides an introduction to new subtopics in mini-reviews. New features also include a new chapter on cell line authentication with a review of the major issues and appropriate protocols including DNA profiling and barcoding, as well as some new specialized protocols. Because of the continuing expansion of cell culture, and to keep the bulk of the book to a reasonable size, some specialized protocols are presented as supplementary material online. Culture of Animal Cells: A Manual of Basic Technique and Specialized Applications, Seventh Edition provides the most accessible and comprehensive introduction available to the culture and experimental manipulation of animal cells. This text is an indispensable resource for those in or entering the field, including academic research scientists, clinical and biopharmaceutical researchers, undergraduate and graduate students, cell and molecular biology and genetics lab managers, trainees and technicians.
Book Synopsis Cell Line Development by : Mohamed Al-Rubeai
Download or read book Cell Line Development written by Mohamed Al-Rubeai and published by Springer Science & Business Media. This book was released on 2009-08-11 with total page 259 pages. Available in PDF, EPUB and Kindle. Book excerpt: Mammalian cell lines command an effective monopoly for the production of therapeutic proteins that require post-translational modifications. This unique advantage outweighs the costs associated with mammalian cell culture, which are far grater in terms of development time and manufacturing when compared to microbial culture. The development of cell lines has undergone several advances over the years, essentially to meet the requirement to cut the time and costs associated with using such a complex hosts as production platforms. This book provides a comprehensive guide to the methodology involved in the development of cell lines and the cell engineering approach that can be employed to enhance productivity, improve cell function, glycosylation and secretion and control apoptosis. It presents an overall picture of the current topics central to expression engineering including such topics as epigenetics and the use of technologies to overcome positional dependent inactivation, the use of promoter and enhancer sequences for expression of various transgenes, site directed engineering of defined chromosomal sites, and examination of the role of eukaryotic nucleus as the controller of expression of genes that are introduced for production of a desired product. It includes a review of selection methods for high producers and an application developed by a major biopharmaceutical industry to expedite the cell line development process. The potential of cell engineering approch to enhance cell lines through the manipulation of single genes that play important roles in key metabolic and regulatory pathways is also explored throughout.
Book Synopsis The Joy of Science by : Richard A. Lockshin
Download or read book The Joy of Science written by Richard A. Lockshin and published by Springer Science & Business Media. This book was released on 2007-11-05 with total page 426 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book reveals that scientific logic is an extension of common, everyday logic and that it can and should be understood by everyone. Written by a practicing and successful scientist, it explores why questions arise in science and looks at how questions are tackled, what constitutes a valid answer, and why. The author does not bog the reader down in technical details or lists of facts to memorize. He uses accessible examples, illustrations, and descriptions to address complex issues. The book should prove enlightening to anyone who has been perplexed by the meaning, relevance, and moral or political implications of science.
Book Synopsis PCR Cloning Protocols by : Bing-Yuan Chen
Download or read book PCR Cloning Protocols written by Bing-Yuan Chen and published by Springer Science & Business Media. This book was released on 2008-02-05 with total page 429 pages. Available in PDF, EPUB and Kindle. Book excerpt: PCR Cloning Protocols, Second Edition, updates and expands Bruce White's best-selling PCR Cloning Protocols (1997) with the newest procedures for DNA cloning and mutagenesis. Here the researcher will find readily reproducible methods for all the major aspects of PCR use, including PCR optimization, computer programs for PCR primer design and analysis, and novel variations for cloning genes of special characteristics or origin, with emphasis on long distance PCR and GC-rich template amplification. Also included are both conventional and novel enzyme-free and restriction site-free procedures to clone PCR products into a range of vectors, as well as state-of-the-art protocols to facilitate DNA mutagenesis and recombination, and to clone the challenging uncharacterized DNA flanking a known DNA fragment.
Download or read book Cloned Ambition written by Gail Daley and published by Gail Daleys Fine Art. This book was released on 2023-11-30 with total page 212 pages. Available in PDF, EPUB and Kindle. Book excerpt: “A Modern twist on the story of David and Bathsheba.” Set in a future where clones are seen as second-class citizens and Normals are threatened by their own creation, Scarlet and her Mate Dagmar must fight for their own freedom and survival. Scarlet was created to be the vessel for an aging actress's brain when she reached maturity. But when her creator dies before the transplant can take place, Scarlet is sold to a clone breeding farm, and only narrowly escapes a terrible fate to rejoin her mate Dagmar and his band of ‘wild’ clones. Desperate for a different future, Scarlet joins her mate Dagmar and his band of 'Wild' Clones, who have rebelled against their masters’ and are trying to build their own society. But when their leader, Napoleon, makes a deal with the illegal Portal gatekeepers to find a world for the Freed Clones colony, Scarlet discovers there is a heavy price for her dream of freedom. Napoleon wants Scarlet. She will be forced to choose between her mate Dagmar who she loves or giving in to Napoleon’s demands. To protect the free clone colony on Halcyon, and with danger lurking around every corner, Scarlet must make a stand for herself and all woman clones for the right to choose who is her mate. If you enjoyed the suspense and adventure of The Hunger Games, then you'll be captivated by Scarlet's journey for freedom in this thrilling novel.
Download or read book Epac'90 written by and published by Atlantica Séguier Frontières. This book was released on 1990 with total page 160 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Antibody Engineering by : Thomas Böldicke
Download or read book Antibody Engineering written by Thomas Böldicke and published by BoD – Books on Demand. This book was released on 2018-02-21 with total page 314 pages. Available in PDF, EPUB and Kindle. Book excerpt: Antibody Engineering comprises in vitro selection and modification of human antibodies including humanization of mouse antibodies for therapy, diagnosis, and research. This book comprises an overview about the generation of antibody diversity and essential techniques in antibody engineering: construction of immune, naive and synthetic libraries, all available in vitro display methods, humanization by chain shuffling, affinity maturation techniques, de novo synthesis of antibody genes, colony assays for library screening, construction of scFvs from hybridomas, and purification of monoclonal antibodies by exclusion chromatography. In addition, other topics that are discussed in this book are application and mechanism of single domain antibodies, structural diversity of antibodies, immune-mediated skin reactions induced by TNF-alpha recombinant antibodies, and bioinformatic approaches to select pathogen-derived peptide sequences for antibody targets.
Book Synopsis Rapid Diagnosis in Populations at Risk from Radiation and Chemicals by : Antonina Cebulska-Wasilewska
Download or read book Rapid Diagnosis in Populations at Risk from Radiation and Chemicals written by Antonina Cebulska-Wasilewska and published by IOS Press. This book was released on 2010 with total page 392 pages. Available in PDF, EPUB and Kindle. Book excerpt: It is a reality that we live in a world where populations could be exposed to radiation or hazardous chemicals as a result of an accident, act of terrorism or a war. This exposure could cause serious health problems, sometimes within days or weeks, and may even lead to a latent health effect on humans, such as an increased cancer rate. Health professionals dealing with these cases therefore need methods to measure the effects of radiation and chemicals using molecular & cellular assays, and their possible short & long term consequences for potentially exposed members of a population at risk. Many biological methods are currently available to monitor both the environment and humans following exposure to physical & chemical agents, by using specific biomarkers to measure genetic damage and changes in macromolecules and body tissues. This makes it possible to perform a reliable risk assessment and to protect the public against unwanted consequences of terrorism and other threats...
Book Synopsis Essentials of Stem Cell Biology by : Robert Lanza
Download or read book Essentials of Stem Cell Biology written by Robert Lanza and published by Academic Press. This book was released on 2009-06-05 with total page 681 pages. Available in PDF, EPUB and Kindle. Book excerpt: First developed as an accessible abridgement of the successful Handbook of Stem Cells, Essentials of Stem Cell Biology serves the needs of the evolving population of scientists, researchers, practitioners and students that are embracing the latest advances in stem cells. Representing the combined effort of seven editors and more than 200 scholars and scientists whose pioneering work has defined our understanding of stem cells, this book combines the prerequisites for a general understanding of adult and embryonic stem cells with a presentation by the world's experts of the latest research information about specific organ systems. From basic biology/mechanisms, early development, ectoderm, mesoderm, endoderm, methods to application of stem cells to specific human diseases, regulation and ethics, and patient perspectives, no topic in the field of stem cells is left uncovered. - Selected for inclusion in Doody's Core Titles 2013, an essential collection development tool for health sciences libraries - Contributions by Nobel Laureates and leading international investigators - Includes two entirely new chapters devoted exclusively to induced pluripotent stem (iPS) cells written by the scientists who made the breakthrough - Edited by a world-renowned author and researcher to present a complete story of stem cells in research, in application, and as the subject of political debate - Presented in full color with glossary, highlighted terms, and bibliographic entries replacing references
Book Synopsis Current Protocols Essential Laboratory Techniques by : Sean R. Gallagher
Download or read book Current Protocols Essential Laboratory Techniques written by Sean R. Gallagher and published by John Wiley & Sons. This book was released on 2012-03-19 with total page 679 pages. Available in PDF, EPUB and Kindle. Book excerpt: The latest title from the acclaimed Current Protocols series, Current Protocols Essential Laboratory Techniques, 2e provides the new researcher with the skills and understanding of the fundamental laboratory procedures necessary to run successful experiments, solve problems, and become a productive member of the modern life science laboratory. From covering the basic skills such as measurement, preparation of reagents and use of basic instrumentation to the more advanced techniques such as blotting, chromatography and real-time PCR, this book will serve as a practical reference manual for any life science researcher. Written by a combination of distinguished investigators and outstanding faculty, Current Protocols Essential Laboratory Techniques, 2e is the cornerstone on which the beginning scientist can develop the skills for a successful research career.
Book Synopsis Genomes of Plants and Animals by : J. Perry Gustafson
Download or read book Genomes of Plants and Animals written by J. Perry Gustafson and published by Springer Science & Business Media. This book was released on 2013-06-29 with total page 320 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume brings together the disciplines of plant and animal genome research, and serves as an opportunity for scientists from both fields to compare results, problems and prospects.
Book Synopsis Recombinant DNA Principles and Methodologies by : James Greene
Download or read book Recombinant DNA Principles and Methodologies written by James Greene and published by CRC Press. This book was released on 1998-06-25 with total page 776 pages. Available in PDF, EPUB and Kindle. Book excerpt: This comprehensive yet balanced work emphasizes the principles and rationale underlying recombinant DNA methodology while furnishing a general understanding of the experimental protocols-suggesting flexible approaches to resolving particular molecular necessities that are easily adaptable to readers' specific applications. Features summary tables presenting at-a-glance information on practices of recombinant DNA methodologies! Recombinant DNA Principles and Methodologies discusses basic and advanced topics requisite to the employment of recombinant DNA technology, such as plasmid biology nucleic acid biochemistry restriction enzymes cloning strategies gel electrophoresis southern and northern blotting preparation of probes phage lambda biology cosmids and genome analysis cloned gene expression polymerase chain reaction conventional and automated DNA sequencing site-directed mutagenesis and more! Elucidating the material with over 2250 edifying references, equations, drawings, and photographs, this state-of-the-art resource is a valuable hands-on guide for molecular and cell biologists, biochemists, bioprocess technologists, applied and industrial microbiologists, virologists, geneticists, chemical engineers, and upper-level undergraduate and graduate students in these disciplines.
Book Synopsis Molecular Biology and Biotechnology by : Ralph Rapley
Download or read book Molecular Biology and Biotechnology written by Ralph Rapley and published by Royal Society of Chemistry. This book was released on 2015-11-09 with total page 574 pages. Available in PDF, EPUB and Kindle. Book excerpt: One of the exciting aspects of being involved in the field of molecular biology is the ever-accelerating rate of progress, both in the development of new methodologies and the practical applications of these methodologies. This popular textbook has been completely revised and updated to provide a comprehensive overview and to reflect key developments in this rapidly expanding area. Chapters on the impact of molecular biology in the development of biotechnology have been fully updated and include the applications of molecular biology in the areas of diagnostics, biosensors and biomarkers, therapeutics, agricultural biotechnology and vaccines. The first six chapters deal with the technology used in current molecular biology and biotechnology. These primarily deal with core nucleic acid techniques, genomics, proteomics and recombinant protein production. Further chapters address major advances in the applications of molecular biotechnology. By presenting information in an easily assimilated form, this book makes an ideal undergraduate text. Molecular Biology and Biotechnology 6th Edition will be of particular interest to students of biology and chemistry, as well as to postgraduates and other scientific workers who need a sound introduction to this ever rapidly advancing and expanding area.
Book Synopsis Journal by : National Cancer Institute (U.S.)
Download or read book Journal written by National Cancer Institute (U.S.) and published by . This book was released on 1972 with total page 968 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Cell Biology written by Julio E. Celis and published by Elsevier. This book was released on 2006 with total page 632 pages. Available in PDF, EPUB and Kindle. Book excerpt: This four-volume laboratory manual contains comprehensive state-of-the-art protocols essential for research in the life sciences. Techniques are presented in a friendly step-by-step fashion, providing useful tips and potential pitfalls. The important steps and results are beautifully illustrated for further ease of use. This collection enables researchers at all stages of their careers to embark on basic biological problems using a variety of technologies and model systems. This thoroughly updated third edition contains 165 new articles in classical as well as rapidly emerging technologies. Topics covered include: Cell and Tissue Culture: Associated Techniques, Viruses, Antibodies, Immunocytochemistry (Volume 1) Organelle and Cellular Structures, Assays (Volume 2) Imaging Techniques, Electron Microscopy, Scanning Probe and Scanning Electron Microscopy, Microdissection, Tissue Arrays, Cytogenetics and In Situ Hybridization, Genomics and Transgenic Knockouts and Knock-down Methods (Volume 3) Transfer of Macromolecules, Expression Systems, Gene Expression Profiling (Volume 4) Indispensable bench companion for every life science laboratory Provides the latest information on the plethora of technologies needed to tackle complex biological problems Includes numerous illustrations, some in full color, supporting steps and results
Book Synopsis Journal of the National Cancer Institute by :
Download or read book Journal of the National Cancer Institute written by and published by . This book was released on 2001 with total page 690 pages. Available in PDF, EPUB and Kindle. Book excerpt:
