Characterizing Bacterial Secretion Systems Using Mass Spectrometry

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Publisher : LAP Lambert Academic Publishing
ISBN 13 : 9783847321194
Total Pages : 212 pages
Book Rating : 4.3/5 (211 download)

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Book Synopsis Characterizing Bacterial Secretion Systems Using Mass Spectrometry by : Pragya Singh

Download or read book Characterizing Bacterial Secretion Systems Using Mass Spectrometry written by Pragya Singh and published by LAP Lambert Academic Publishing. This book was released on 2012-06 with total page 212 pages. Available in PDF, EPUB and Kindle. Book excerpt: Gram-negative bacteria have evolved an array of secretion systems for protein transport across their membranes. The ability to secrete a diverse set of proteins to their target of choice provides bacteria with a menacing tool for pathogenesis. This work aims to enhance our understanding of bacterial pathogenesis by characterizing key aspects of bacterial secretion systems using mass spectrometry. Chapter 1 provides a general introduction of protein secretion pathways in Gran-negative bacteria, with a special emphasis on type III and type VI secretion systems. Chapter 2 highlights chemical cross-linking and mass spectrometry (CXMS) as a technique for studying protein-protein interactions in large molecular complexes. Chapter 3 describes a novel pipeline for acquisition and analysis of CXMS data. In Chapter 4, key interactions between the basal components of type III secretion systems (T3SS) are identified, and used to create a model of the core structure of T3SS. In Chapter 5, secretome of T6SS in P. aeruginosa is profiled to identify three novel substrates of the system. Evidence of the involvement of one of these substrates in inter-bacterial interactions is presented.

Structural and Functional Characterization of Bacterial Secretion Systems Using Mass Spectrometry

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Publisher :
ISBN 13 :
Total Pages : 388 pages
Book Rating : 4.:/5 (755 download)

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Book Synopsis Structural and Functional Characterization of Bacterial Secretion Systems Using Mass Spectrometry by : Pragya Singh

Download or read book Structural and Functional Characterization of Bacterial Secretion Systems Using Mass Spectrometry written by Pragya Singh and published by . This book was released on 2011 with total page 388 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Identification of Microorganisms by Mass Spectrometry

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Publisher : John Wiley & Sons
ISBN 13 : 0471748633
Total Pages : 377 pages
Book Rating : 4.4/5 (717 download)

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Book Synopsis Identification of Microorganisms by Mass Spectrometry by : Charles L. Wilkins

Download or read book Identification of Microorganisms by Mass Spectrometry written by Charles L. Wilkins and published by John Wiley & Sons. This book was released on 2005-12-16 with total page 377 pages. Available in PDF, EPUB and Kindle. Book excerpt: A multidisciplinary approach to understanding the fundamentals of mass spectrometry for bacterial analysis From chemotaxonomy to characterization of targeted proteins, Identification of Microorganisms by Mass Spectrometry provides an overview of both well-established and cutting-edge mass spectrometry techniques for identifying microorganisms. A vital tool for microbiologists, health professionals, and analytical chemists, the text is designed to help scientists select the most effective techniques for use in biomedical, biochemical, pharmaceutical, and bioterror defense applications. Since microbiological applications of mass spectrometry require a basic understanding of both microbiology and analytical chemistry, the editors have incorporated material from both disciplines so that readers from either field will come to understand the necessary principles of the other. Featuring contributions from some of the most recognized experts in both fields, this volume provides specific examples of fundamental methods as well as approaches developed in the last decade, including: * Metastable atom bombardment pyrolysis mass spectrometry * Matrix-assisted laser desorption/ionization mass spectrometry (MALDI) * MALDI time-of-flight mass spectrometry (MALDI-TOF MS) of intact bacteria * High-resolution Fourier transform mass spectrometry (FTMS) * Electrospray ionization (ESI) mass spectrometry Identification of Microorganisms by Mass Spectrometry represents the most comprehensive and up-to-date work on the topic currently available. It is liberally illustrated with figures and tables and covers every aspect of spectrometric identification of microorganisms, including experimental procedures, various means of sample preparation, data analysis, and interpretation of complex mass spectral data.

Applications of Mass Spectrometry in Microbiology

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Publisher : Springer
ISBN 13 : 3319260707
Total Pages : 336 pages
Book Rating : 4.3/5 (192 download)

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Book Synopsis Applications of Mass Spectrometry in Microbiology by : Plamen Demirev

Download or read book Applications of Mass Spectrometry in Microbiology written by Plamen Demirev and published by Springer. This book was released on 2016-01-12 with total page 336 pages. Available in PDF, EPUB and Kindle. Book excerpt: In the last quarter century, advances in mass spectrometry (MS) have been at the forefront of efforts to map complex biological systems including the human metabolome, proteome, and microbiome. All of these developments have allowed MS to become a well-established molecular level technology for microorganism characterization. MS has demonstrated its considerable advantage as a rapid, accurate, and cost-effective method for microorganism identification, compared to conventional phenotypic techniques. In the last several years, applications of MS for microorganism characterization in research, clinical microbiology, counter-bioterrorism, food safety, and environmental monitoring have been documented in thousands of publications. Regulatory bodies in Europe, the US, and elsewhere have approved MS-based assays for infectious disease diagnostics. As of mid-2015, more than 3300 commercial MS systems for microorganism identification have been deployed worldwide in hospitals and clinical labs. While previous work has covered broader approaches in using MS to characterize microorganisms at the species level or above, this book focuses on strain-level and subtyping applications. In twelve individual chapters, innovators, leaders and practitioners in the field from around the world have contributed to a comprehensive overview of current and next-generation approaches for MS-based microbial characterization at the subspecies and strain levels. Chapters include up-to-date reference lists as well as web-links to databases, recommended software, and other useful tools. The emergence of new, antibiotic-resistant strains of human or animal pathogens is of extraordinary concern not only to the scientific and medical communities, but to the general public as well. Developments of novel MS-based assays for rapid identification of strains of antibiotic-resistant microorganisms are reviewed in the book as well. Microbiologists, bioanalytical scientists, infectious disease specialists, clinical laboratory and public health practitioners as well as researchers in universities, hospitals, government labs, and the pharmaceutical and biotechnology industries will find this book to be a timely and valuable resource.

The Use of Mass Spectrometry to Characterize the Metabolic Output of Bacterial Pathogens

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Publisher :
ISBN 13 : 9781124554891
Total Pages : 172 pages
Book Rating : 4.5/5 (548 download)

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Book Synopsis The Use of Mass Spectrometry to Characterize the Metabolic Output of Bacterial Pathogens by : David Junior Gonzalez

Download or read book The Use of Mass Spectrometry to Characterize the Metabolic Output of Bacterial Pathogens written by David Junior Gonzalez and published by . This book was released on 2011 with total page 172 pages. Available in PDF, EPUB and Kindle. Book excerpt: The overall theme of this thesis is the use of mass spectrometry to answer questions of biological relevance. The thesis begins by informing the reader on the basics of mass spectrometry and associated tools with an introductory chapter. Chapter 2 describes collaborative work performed between three labs at UCSD, the Jack E. Dixon, Victor Nizet and Pieter C. Dorrestein labs. The project was initiated by the use of comparative genomics to discover a widely distribution toxin biosynthetic gene cluster. Molecular and biochemical analysis of a two members of the conserved family, namely Group A Streptococcus and Clostridium Botulinum, demonstrated the organisms indeed contained similar cellular machinery for the biosynthesis of ribosomally encoded peptides putatively containing thiazole and (methyl)-oxazole moieties. Chapter 3 continues characterizing the widely distributed family of toxins specifically characterizing the gene cluster within the bacterium C. Botulinum. The work describes the use of mass spectrometry to obtain structural evidence, verifying the biosynthetic gene cluster indeed produces heterocycles as hypothesized by the genomic studies. Additionally, the work uses biochemical and genetic approaches to characterize a novel [Beta]-hemolytic, cytolytic toxin in two Clostridia species. Chapter 4 introduces the method of imaging mass spectrometry (IMS) of microbial colonies. Although IMS is not a novel technique, very few reports had used the technology to investigate the microbial kingdom. The work within chapter 4 describes an IMS survey performed on a vast number of differential microbes to visualize their associated metabolic output. The goal was to show that IMS is an amenable technique that can be applied to a diverse number of microbes. Chapter 5 goes beyond the proposal set forth in chapter 4, as IMS was used to visualize the interaction between two well characterized model bacterial systems, the probiotic bacterium Bacillus subtilis and the bona fide human pathogen methicillin resistant Staphylococcus aureus (MRSA). Imaging mass spectrometry showed directional release of compounds by the probiotic organism B. subtilis that have antibiotic effects against MRSA giving insight into the potential mode of action for probiotic organisms.

Development and Application of Mass Spectrometry in Monitoring of Chemical Reactions and Rapid Characterization of Pathogenic Bacteria

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Publisher :
ISBN 13 :
Total Pages : pages
Book Rating : 4.:/5 (897 download)

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Book Synopsis Development and Application of Mass Spectrometry in Monitoring of Chemical Reactions and Rapid Characterization of Pathogenic Bacteria by : Yu-Chie Chen

Download or read book Development and Application of Mass Spectrometry in Monitoring of Chemical Reactions and Rapid Characterization of Pathogenic Bacteria written by Yu-Chie Chen and published by . This book was released on 2013 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:

Global Strategy for Health for All by the Year 2000

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Publisher :
ISBN 13 : 9789241800037
Total Pages : 90 pages
Book Rating : 4.8/5 ( download)

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Book Synopsis Global Strategy for Health for All by the Year 2000 by : World Health Organization

Download or read book Global Strategy for Health for All by the Year 2000 written by World Health Organization and published by . This book was released on 1981-01-01 with total page 90 pages. Available in PDF, EPUB and Kindle. Book excerpt:

The Use of Mass Spectrometry Technology (MALDI-TOF) in Clinical Microbiology

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Publisher : Academic Press
ISBN 13 : 0128144521
Total Pages : 299 pages
Book Rating : 4.1/5 (281 download)

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Book Synopsis The Use of Mass Spectrometry Technology (MALDI-TOF) in Clinical Microbiology by : Fernando Cobo

Download or read book The Use of Mass Spectrometry Technology (MALDI-TOF) in Clinical Microbiology written by Fernando Cobo and published by Academic Press. This book was released on 2018-08-03 with total page 299 pages. Available in PDF, EPUB and Kindle. Book excerpt: The Use of Mass Spectrometry Technology (MALDI-TOF) in Clinical Microbiology presents the state-of the-art for MALDI-TOF mass spectrometry. It is a key reference defining how MALDI-TOF mass spectrometry is used in clinical settings as a diagnostic tool of microbial identification and characterization that is based on the detection of a mass of molecules. The book provides updated applications of MALDI-TOF techniques in clinical microbiology, presenting the latest information available on a technology that is now used for rapid microbial identification at relatively low cost, thus offering an alternative to conventional laboratory diagnosis and proteomic identification systems. Although the main use of the technology has, until now, been identification or typing of bacteria from a positive culture, applications in the field of virology, mycology, microbacteriology and resistances are opening up new opportunities. Presents updated applications of MALDI-TOF techniques in clinical microbiology Describes the use of mass spectrometry in the lab, the principles of the technology, preparation of samples, device calibration and maintenance, treatment of microorganisms, and quality control Presents key information for researchers, including possible uses of the technology, differences between devices, how to interpret results, and future applications Covers the topic in a systematic and comprehensive manner that is useful to both clinicians and researchers

Metabolic Profiling

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Publisher : Humana
ISBN 13 : 9781493992522
Total Pages : 291 pages
Book Rating : 4.9/5 (925 download)

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Book Synopsis Metabolic Profiling by : Georgios A. Theodoridis

Download or read book Metabolic Profiling written by Georgios A. Theodoridis and published by Humana. This book was released on 2019-04-30 with total page 291 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume explores the different approaches and techniques used by researchers to study the recent challenges and developments in metabolic profiling. This book is divided into IV parts. Part I contains chapters that highlight basic concepts, such as experimental design, data treatment, metabolite identification, and harmonization. Part II describes experimental protocols for both targeted and untargeted metabolomics covering the basic analytical technologies: LC-MS, GC-MS, NMR and CE-MS. In addition the protocols describe methods for the study of tissues, feces, blood and other types of biological samples as well as the application of chemical derivatization for GC-MS. Parts III and IV present the use of metabolomics in the study of food, plants and the life sciences, with examples from the quest for the discovery of disease biomarkers, physical exercise omics and metabolic profiling of food, fruit and wine. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and thorough, Metabolic Profiling: Methods and Protocols is a valuable resource for researchers who are interested in expanding their knowledge of this rapidly developing field.

Bacterial Secretion Systems

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Publisher : Springer Nature
ISBN 13 : 1071634453
Total Pages : 607 pages
Book Rating : 4.0/5 (716 download)

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Book Synopsis Bacterial Secretion Systems by : Laure Journet

Download or read book Bacterial Secretion Systems written by Laure Journet and published by Springer Nature. This book was released on 2023-11-06 with total page 607 pages. Available in PDF, EPUB and Kindle. Book excerpt: This second edition details new and updated protocols that cover techniques used to study secretion systems. Chapters focus on identifying and localizing the different subunits, defining interactions within subunits, monitoring conformational changes, purifying and imaging of large complexes, defining the assembly pathway by fluorescence microscopy and the role of energy during assembly and/or secretion, identifying secreted effectors as well as using reporters to follow effector transport. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Bacterial Secretion Systems: Methods and Protocols- Second Edition aims to be a useful and practical guide to new researchers and experts looking to expand their knowledge.

The Comprehensive Sourcebook of Bacterial Protein Toxins

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Publisher : Elsevier
ISBN 13 : 0080456987
Total Pages : 1072 pages
Book Rating : 4.0/5 (84 download)

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Book Synopsis The Comprehensive Sourcebook of Bacterial Protein Toxins by : Joseph E. Alouf

Download or read book The Comprehensive Sourcebook of Bacterial Protein Toxins written by Joseph E. Alouf and published by Elsevier. This book was released on 2005-12-20 with total page 1072 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book describes the major achievements and discoveries relevant to bacterial protein toxins since the turn of the new century illustrated by the discovery of more than fifty novel toxins (many of them identified through genome screening). The establishment of the three-dimensional crystal structure of more than 20 toxins during the same period offers deeper knowledge of structure-activity relationships and provides a framework to understand how toxins recognize receptors, penetrate membranes and interact with and modify intracellular substrates. Edited by two of the most highly regarded experts in the field from the Institut Pasteur, France 14 brand new chapters dedicated to coverage of historical and general aspects of toxinology Includes the major toxins of both basic and clinical interest are described in depth Details applied aspects of toxins such as therapy, vaccinology, and toolkits in cell biology Evolutionary and functional aspects of bacterial toxins evaluated and summarized Toxin applications in cell biology presented Therapy (cancer therapy, dystonias) discussed Vaccines (native and genetically engineered vaccines) featured Toxins discussed as biological weapons, comprising chapters on anthrax, diphtheria, ricin etc.

Identification and Characterization of Contrasting Genotypes/Cultivars to Discover Novel Players in Crop Responses to Abiotic/Biotic Stresses

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Publisher : Frontiers Media SA
ISBN 13 : 2889740099
Total Pages : 739 pages
Book Rating : 4.8/5 (897 download)

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Book Synopsis Identification and Characterization of Contrasting Genotypes/Cultivars to Discover Novel Players in Crop Responses to Abiotic/Biotic Stresses by : Raul Antonio Sperotto

Download or read book Identification and Characterization of Contrasting Genotypes/Cultivars to Discover Novel Players in Crop Responses to Abiotic/Biotic Stresses written by Raul Antonio Sperotto and published by Frontiers Media SA. This book was released on 2022-02-24 with total page 739 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Mass Spectrometry for Microbial Proteomics

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Publisher : John Wiley & Sons
ISBN 13 : 1119991927
Total Pages : 545 pages
Book Rating : 4.1/5 (199 download)

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Book Synopsis Mass Spectrometry for Microbial Proteomics by : Haroun N. Shah

Download or read book Mass Spectrometry for Microbial Proteomics written by Haroun N. Shah and published by John Wiley & Sons. This book was released on 2010-10-28 with total page 545 pages. Available in PDF, EPUB and Kindle. Book excerpt: New advances in proteomics, driven largely by developments in mass spectrometry, continue to reveal the complexity and diversity of pathogenic mechanisms among microbes that underpin infectious diseases. Therefore a new era in medical microbiology is demanding a rapid transition from current procedures to high throughput analytical systems for the diagnosis of microbial pathogens. This book covers the broad microbiological applications of proteomics and mass spectrometry. It is divided into six sections that follow the general progression in which most microbiology laboratories are approaching the subject –Transition, Tools, Preparation, Profiling by Patterns, Target Proteins, and Data Analysis.

The Mycobacterial Cell Envelope

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Publisher :
ISBN 13 : 9781555814687
Total Pages : 0 pages
Book Rating : 4.8/5 (146 download)

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Book Synopsis The Mycobacterial Cell Envelope by : Mamadou Daffé

Download or read book The Mycobacterial Cell Envelope written by Mamadou Daffé and published by . This book was released on 2008 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Explains the unique characteristics that cause this large group of bacteria responsible for tuberculosis and leprosy to function differently; serves as a valuable reference for those working in the areas of biochemistry, genetics, genomics, and immunology.

Proteomics of Microbial Pathogens

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Publisher : John Wiley & Sons
ISBN 13 : 9783527317592
Total Pages : 352 pages
Book Rating : 4.3/5 (175 download)

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Book Synopsis Proteomics of Microbial Pathogens by : Peter R. Jungblut

Download or read book Proteomics of Microbial Pathogens written by Peter R. Jungblut and published by John Wiley & Sons. This book was released on 2007-01-30 with total page 352 pages. Available in PDF, EPUB and Kindle. Book excerpt: High-quality research articles on proteomic analyses of microbial pathogens, made available in a handy form. Containing proven, high-quality research articles selected from the popular PROTEOMICS journal, this is a current overview of the latest research into the proteomics analysis of microbial pathogens as well as several review articles.

Advancements of Mass Spectrometry in Biomedical Research

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Publisher : Springer
ISBN 13 : 3030159507
Total Pages : 813 pages
Book Rating : 4.0/5 (31 download)

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Book Synopsis Advancements of Mass Spectrometry in Biomedical Research by : Alisa G. Woods

Download or read book Advancements of Mass Spectrometry in Biomedical Research written by Alisa G. Woods and published by Springer. This book was released on 2019-07-25 with total page 813 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume explores the use of mass spectrometry for biomedical applications. Chapters focus on specific therapeutic areas such as oncology, infectious disease, and psychiatry. Additional chapters focus on methodology, technologies and instrumentation, as well as on analysis of protein-protein interactions, protein quantitation, and protein post-translational modifications. Various omics fields such as proteomics, metabolomics, glycomics, lipidomics, and adductomics are also covered. Applications of mass spectrometry in biotechnological and pharmaceutical industry are also discussed. This volume provides readers with a comprehensive and informative manual that will allow them to appreciate mass spectrometry and proteomic research, but also to initiate and improve their own work. This book acts as a technical guide as well as a conceptual guide to the newest information in this exciting field.

Characterization of rare and recently first described human pathogenic bacteria

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Publisher : Frontiers Media SA
ISBN 13 : 2832530192
Total Pages : 127 pages
Book Rating : 4.8/5 (325 download)

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Book Synopsis Characterization of rare and recently first described human pathogenic bacteria by : Percy Schröttner

Download or read book Characterization of rare and recently first described human pathogenic bacteria written by Percy Schröttner and published by Frontiers Media SA. This book was released on 2023-07-24 with total page 127 pages. Available in PDF, EPUB and Kindle. Book excerpt: