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Characterization Of The Integrated Virus Detection System Ivds Using Ms 2 Bacteriophage
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Book Synopsis Characterization of MS2 Bacteriophage by Integrated Virus Detection System (IVDS) Physical Counting Methodology by :
Download or read book Characterization of MS2 Bacteriophage by Integrated Virus Detection System (IVDS) Physical Counting Methodology written by and published by . This book was released on 1999 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: A new physically based methodology - The Integrated Virus Detection System (IVDS) - was used to characterize a high concentration, 10.2 mg protein/ml, sample preparation of MS2 Bacteriophage with a reported 10(exp 14) pfu/ml (DPM14) virus count in a common TNME buffer. Virus counts were made using the IVDS instrument following serial dilution. Results indicated virus counts of 1.5 x 10(exp 5) for the neat sample (DPM14), followed by 6.5 x 10(exp 4) viruses (DPM13), 1.2 x 10(exp 4) viruses (DPM12), 9.3 x 10(exp 2) viruses (DPM11), 88 viruses (DPM10), and 5 viruses (DPM9), respectively. Lower concentrations display a consistent multiplier and were consistent with target dilutions. Increases in virus concentration appear to decrease the multiplier. Variation is considered to be due to aggregation. Results demonstrate a consistent and simple-to-use methodology. Results further indicate that the IVDS instrument can be used for characterization of other virus preparations with equal ease and similar results.
Book Synopsis Characterization of the Integrated Virus Detection System (IVDS) Using MS-2 Bacteriophage by :
Download or read book Characterization of the Integrated Virus Detection System (IVDS) Using MS-2 Bacteriophage written by and published by . This book was released on 1999 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: The detection and analysis of viruses have been goals of science for more than 70 years, following the first real evidence that a new type of microorganism was responsible for diseases in man and animals. These new microbes were smaller than bacteria, which have now been well documented, classified, and studied. Their small size made classifying the new microbes more difficult, and the field of virology has been advanced by biochemical techniques rather than by direct examination. Advancements in electron microscopy in more recent times have made advances in this area, and much has been reported on the physical features of more than 21 virus families. All these historic techniques are time consuming and require special knowledge and specialized chemicals and preparations to be successful. Capitalizing on the physical characteristics, it was possible to separate the families and count the individual viruses in a new and dramatic way, using easily obtained materials and simple-to-operate techniques. The only materials used in the Integrated Virus Detection System (IVDS) are a buffer, CO2 gas, and butanol. The work in this report presents the advances in the concentration and counting phases of IVDS. Results indicate a practical, easy to use device, which can count viruses in near real time. The JVDS can, in its final configuration, be expected to analyze a sample, count the viruses present, and give a preliminary identification for all viruses.
Book Synopsis Mass Spectrometry and Integrated Virus Detection System Characterization of MS2 Bacteriophage by :
Download or read book Mass Spectrometry and Integrated Virus Detection System Characterization of MS2 Bacteriophage written by and published by . This book was released on 2005 with total page 36 pages. Available in PDF, EPUB and Kindle. Book excerpt: This study demonstrates the characterization of the sample matrix composition of the MS2 virus using ESI-MS and IVDS detection systems. The MS2 samples were grown and purified using various techniques, which showed ESI-MS samples responding differently from IVDS samples. The LC-MS of the specific biomarker of the MS2 bacteriophage from an infected Escherichia coli (E. coli) sample was characterized in the presence of E. coli proteins. The significant impact of the sample matrix was observed upon the identification of MS2 using a database search. Escherichia coli infected with MS2 showed a different score from other uninfected scores. Only purified MS2, using CsCl and analyzed by LS-MS, showed a positive match in the database search. However, the variation in the MS2 sample matrix had no effect on the characterization of MS2.
Book Synopsis Stability of the Integrated Virus Detection System (VDS) Electrospray Module During Analysis of MS2 Bacteriophage by :
Download or read book Stability of the Integrated Virus Detection System (VDS) Electrospray Module During Analysis of MS2 Bacteriophage written by and published by . This book was released on 2005 with total page 15 pages. Available in PDF, EPUB and Kindle. Book excerpt: This report characterizes the stability of the electrospray module of the IVDS system while analyzing a sample of MS2 bacteriophage. The electrospray parameters were varied across the stable range, and resultant output from the detector was analyzed.
Book Synopsis Integrated Virus Detection by : Charles H. Wick
Download or read book Integrated Virus Detection written by Charles H. Wick and published by CRC Press. This book was released on 2014-11-13 with total page 260 pages. Available in PDF, EPUB and Kindle. Book excerpt: Multiple viruses can be detected concurrently using the Integrated Virus Detection System (IVDS). Integrated Virus Detection describes this technology and provides many examples of applications including a chapter on viruses found in honeybees with descriptions of seasonal and yearly variation. This straightforward technology can be used to detect known, unknown, and unsequenced viruses collected from environmental and other complex biological sources. This book summarizes more than ten US patents issued for the invention of the IVDS, which is the common name of the electrospray–differential mobility analyzer method. The IVDS is powering mankind’s ability to rapidly detect, measure, and monitor viruses as well as virus-like particles. Three facts make rapid detection possible: virus size, which ranges from 20 to 800 nm.; disparity in each virus species’ particle size thus allowing size data to be used for detection and preliminary identification; and the fact that virus particle density is distinct from other nanoparticles. The IVDS can ascertain the absence of virion particles, thus presenting compelling evidence of a true negative, which is useful in verifying decontamination and other processes. In addition, large numbers of samples may be processed in an automated fashion, providing an excellent means to prescreen them for judicious targeting of subsequent tests such as PCR or the discriminating method for identifying microbes, which is mass spectrometry proteomics.* The book is helpful to anyone interested in virus detection, especially in situations where many viral types may coexist. *Identifying Microbes by Mass Spectrometry Proteomics (CRC Press 2013)
Book Synopsis Determination of MS2 Bacteriophage Stability at Low PH Using the Integrated Virus Detection System (IVDS). by :
Download or read book Determination of MS2 Bacteriophage Stability at Low PH Using the Integrated Virus Detection System (IVDS). written by and published by . This book was released on 2006 with total page 13 pages. Available in PDF, EPUB and Kindle. Book excerpt: This report describes testing of the survivability of MS2 virus under harsh conditions using the Integrated Virus Detection System (IVDS). In this study, the MS2 was subjected to a highly acidic and oxidative environment. Judging by the concentration of the virus particles, the virus survived 66 hr in 0.1 N HNO3 solution without any signs of diminution in the number of virus particles. This suggests that viruses might be able to survive such harsh environmental conditions.
Book Synopsis Determination of MS2 Bacteriophage Stability at High Temperatures Using the Integrated Virus Detection System (IVDS). by :
Download or read book Determination of MS2 Bacteriophage Stability at High Temperatures Using the Integrated Virus Detection System (IVDS). written by and published by . This book was released on 2005 with total page 17 pages. Available in PDF, EPUB and Kindle. Book excerpt: This is the third report in a series of reports that investigates the survivability of the MS2 virus in harsh environments. The first two reports study the effects of pH on the virus. In this report, we study the effect of elevated temperatures on the virus. The stability of the virus was studied by counting the number of virus particles using the Integrated Virus Detection System (IVDS). Test results show that the virus particles are relatively stable for a limited time (up to 0.50 hr) at temperatures as high as 63 degrees C. Longer exposure or exposure to higher temperatures, results in quick breakdown of the virus particles.
Book Synopsis Integrated Virus Detection by : Charles H. Wick
Download or read book Integrated Virus Detection written by Charles H. Wick and published by CRC Press. This book was released on 2014-11-13 with total page 254 pages. Available in PDF, EPUB and Kindle. Book excerpt: Multiple viruses can be detected concurrently using the Integrated Virus Detection System (IVDS). Integrated Virus Detection describes this technology and provides many examples of applications including a chapter on viruses found in honeybees with descriptions of seasonal and yearly variation. This straightforward technology can be used to detect
Book Synopsis Integrated Virus Detection System Characterization of MS2 and TBSV After Pulsed Lamp Exposure by :
Download or read book Integrated Virus Detection System Characterization of MS2 and TBSV After Pulsed Lamp Exposure written by and published by . This book was released on 2010 with total page 29 pages. Available in PDF, EPUB and Kindle. Book excerpt: The ability to decontaminate microorganisms with a broad band pulsed light system has been demonstrated (ERDC-TR-456). This study will allow the effects of the pulsed lamp decontamination effects on MS2 bacteriophagc and tomato bushy stunt virus to be analyzed quickly without historical microbiological techniques. The Integrated Virus Detection System can analyze multiple samples quickly and allows rapid determination of pulsed lamp effects.
Book Synopsis Removing Complex Growth Media from MS2 Bacteriophage Cultures by :
Download or read book Removing Complex Growth Media from MS2 Bacteriophage Cultures written by and published by . This book was released on 1999 with total page 22 pages. Available in PDF, EPUB and Kindle. Book excerpt: Purification and concentration of viruses from the background material are required whatever the subsequent analysis methods used. For the analysis of viruses, it is essential, and detection methods depend on this solution. This report demonstrates a methodology for removing growth media from a virus preparation. A sample of MS2 was purified using a new ultrafiltration (UF) technique with hollow fibers. A typical MS2 virus sample with a nominal stated concentration of 1.4x10(exp 12) plaque forming units per ml (pfu/ml) in the original growth media was used to demonstrate this method. After UF, the growth media was removed and the viruses counted using the Integrated Virus Detection System (IVDS) instrument. This report further describes the use of this ultrafiltration procedure to remove other impurities, such as cesium chloride and albumin, from solutions containing a purified solution of MS2 bacteriophage. These solutions were also analyzed using the IVDS instrument.
Book Synopsis Virus Detection by : Charles H. Wick
Download or read book Virus Detection written by Charles H. Wick and published by CRC Press. This book was released on 2023-05-03 with total page 158 pages. Available in PDF, EPUB and Kindle. Book excerpt: Viruses do not behave as other microbes; their life cycles require infecting healthy cells, commandeering their cellular apparatus, replicating and then killing the host cell. Methods for virus detection and identification have been developed only in the past few decades. These recently developed methods include molecular, physical, and proteomic techniques. All these approaches (Electron Microscopy, Molecular, Direct Counting, and Mass Spectrometry Proteomics) to detection and identification are reviewed in this succinct volume. It is written in approachable language with enough detail for trained professionals to follow and want to recommend to others. Key Features Covers common detection methods Reviews the history of detection from antiquity to the present Documents the strengths and weaknesses of various detection methods Describes how to detect newly discovered viruses Recommends specific applications for clinical, hospital, environmental, and public health uses
Book Synopsis Single-stranded RNA phages by : Paul Pumpens
Download or read book Single-stranded RNA phages written by Paul Pumpens and published by CRC Press. This book was released on 2020-02-03 with total page 1832 pages. Available in PDF, EPUB and Kindle. Book excerpt: This is a comprehensive guide to single-stranded RNA phages (family Leviviridae), first discovered in 1961. These phages played a unique role in early studies of molecular biology, the genetic code, translation, replication, suppression of mutations. Special attention is devoted to modern applications of the RNA phages and their products in nanotechnology, vaccinology, gene discovery, evolutionary and environmental studies. Included is an overview of the generation of novel vaccines, gene therapy vectors, drug delivery, and diagnostic tools exploring the role of RNA phage-derived products in the revolutionary progress of the protein tethering and bioimaging protocols. Key Features Presents the first full guide to single-stranded RNA phages Reviews the history of molecular biology summarizing the role RNA phages in the development of the life sciences Demonstrates how RNA phage-derived products have resulted in nanotechnological applications Presents an up-to-date account of the role played by RNA phages in evolutionary and environmental studies
Book Synopsis Microfluidic Chip-Capillary Electrophoresis Devices by : Ying Sing Fung
Download or read book Microfluidic Chip-Capillary Electrophoresis Devices written by Ying Sing Fung and published by CRC Press. This book was released on 2015-08-18 with total page 392 pages. Available in PDF, EPUB and Kindle. Book excerpt: Capillary electrophoresis (CE) and microfluidic chip (MC) devices are relatively mature technologies, but this book demonstrates how they can be integrated into a single, revolutionary device that can provide on-site analysis of samples when laboratory services are unavailable. By introducing the combination of CE and MC technology, Microfluidic Ch
Book Synopsis Proteomics for Biological Discovery by : Timothy D. Veenstra
Download or read book Proteomics for Biological Discovery written by Timothy D. Veenstra and published by John Wiley & Sons. This book was released on 2019-05-07 with total page 408 pages. Available in PDF, EPUB and Kindle. Book excerpt: An update to the popular guide to proteomics technology applications in biomedical research Building on the strength of the original edition, this book presents the state of the art in the field of proteomics and offers students and scientists new tools and techniques to advance their own research. Written by leading experts in the field, it provides readers with an understanding of new and emerging directions for proteomics research and applications. Proteomics for Biological Discovery begins by discussing the emergence of proteomics technologies and summarizing the potential insights to be gained from proteome-level research. The tools of proteomics, from conventional to novel techniques, are thoroughly covered, from underlying concepts to limitations and future directions. Later chapters provide an overview of the current developments in post-translational modification studies, structural proteomics, biochemical proteomics, applied proteomics, and bioinformatics relevant to proteomics. Chapters cover: Quantitative Proteomics for Differential Protein Expression Profiling; Protein Microarrays; Protein Biomarker Discovery; Biomarker Discovery using Mass Spectrometry Imaging; Protein-Protein Interactions; Mass Spectrometry Of Intact Protein Complexes; Crosslinking Applications in Structural Proteomics; Functional Proteomics; High Resolution Interrogation of Biological Systems via Mass Cytometry; Characterization of Drug-Protein Interactions by Chemoproteomics; Phosphorylation; Large-Scale Phosphoproteomics; and Probing Glycoforms of Individual Proteins Using Antibody-Lectin Sandwich Arrays. Presents a comprehensive and coherent review of the major issues in proteomic technology development, bioinformatics, strategic approaches, and applications Chapters offer a rigorous overview with summary of limitations, emerging approaches, questions, and realistic future industry and basic science applications Features new coverage of mass spectrometry for high throughput proteomic measurements, and novel quantitation strategies such as spectral counting and stable isotope labeling Discusses higher level integrative aspects, including technical challenges and applications for drug discovery Offers new chapters on biomarker discovery, global phosphorylation analysis, proteomic profiling using antibodies, and single cell mass spectrometry Proteomics for Biological Discovery is an excellent advanced resource for graduate students, postdoctoral fellows, and scientists across all the major fields of biomedical science.
Book Synopsis Neutron Scattering in Biology by : Jörg Fitter
Download or read book Neutron Scattering in Biology written by Jörg Fitter and published by Springer Science & Business Media. This book was released on 2006-05-01 with total page 571 pages. Available in PDF, EPUB and Kindle. Book excerpt: The advent of new neutron facilities and the improvement of existing sources and instruments world wide supply the biological community with many new opportunities in the areas of structural biology and biological physics. The present volume offers a clear description of the various neutron-scattering techniques currently being used to answer biologically relevant questions. Their utility is illustrated through examples by some of the leading researchers in the field of neutron scattering. This volume will be a reference for researchers and a step-by-step guide for young scientists entering the field and the advanced graduate student.
Book Synopsis Bacteriophage T4 by : Christopher K. Mathews
Download or read book Bacteriophage T4 written by Christopher K. Mathews and published by . This book was released on 1983 with total page 432 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Bacteriophage written by Stephen Mc Grath and published by Caister Academic Press Limited. This book was released on 2007 with total page 362 pages. Available in PDF, EPUB and Kindle. Book excerpt: Written by eminent international researchers actively involved in the disparate areas of bacteriophage research this book focuses on the current rapid developments in this exciting field.
