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Characterization Of Post Translational Modification And Protein Protein Interaction Of Rna Binding Protein 1 Rbp1p In Saccharomyces Cerevisiae
Download Characterization Of Post Translational Modification And Protein Protein Interaction Of Rna Binding Protein 1 Rbp1p In Saccharomyces Cerevisiae full books in PDF, epub, and Kindle. Read online Characterization Of Post Translational Modification And Protein Protein Interaction Of Rna Binding Protein 1 Rbp1p In Saccharomyces Cerevisiae ebook anywhere anytime directly on your device. Fast Download speed and no annoying ads. We cannot guarantee that every ebooks is available!
Book Synopsis Candida Albicans by : Rajendra Prasad
Download or read book Candida Albicans written by Rajendra Prasad and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 273 pages. Available in PDF, EPUB and Kindle. Book excerpt: Candida, which was discovered more than a century ago as a causative organism of oral thrush, is now thought to potentially infect almost every tissue of the human body. Although we still do not have a safe anti-candida drug, the growing pace of progess of research on Candida albicans holds promise that a breakthrough is imminent. Though many monographs and articles on candida and candidoses have appeared in recent years, they mostly cover the clinical aspects. This particular text, however, explains the more basic features of candida including the molecular genetics, molecular biology and immunology of the cell wall, the molecular basis of morphogenesis and the structure and function of the plasma membrane. The role of anti-candida drugs and their mechanism of action are also discussed.
Book Synopsis Eukaryotic MRNA Processing by : Adrian Krainer
Download or read book Eukaryotic MRNA Processing written by Adrian Krainer and published by IRL Press. This book was released on 1997 with total page 408 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume focuses on the major aspects of post-transcriptional mRNA processing in the nucleus of eukaryotic cells. Each of the described mRNA reactions is required for proper gene expression and can also serve as a control point for regulating the expression of many genes, for example duringembryonic development or in different cell types. The different chapters review the assembly of newly synthesized nuclear mRNA transcripts into hnRNP particles and catalytically active spliceosomes; the structure and mechanism of action of small nuclear ribonucleoprotein particles and proteinfactors that catalyse pre-mRNA splicing in mammalian cells and in yeast; the regulation of gene expression and generation of protein isoform diversity by alternative splicing; the mechanisms of 3' end cleavage and polyadenylation; the architecture of the cell nucleus in relation to these processesand to the localization of the relevant substrates and factors; the diverse mechanisms of RNA processing by ribozymes and their potential relevance for nuclear mRNA processing; the mechanism of spliced-leader addition by trans-splicing in nematodes and trypanosomes; and the process ofinsertion/deletion mRNA editing in kinetoplasmid protozoa. In each chapter, leading researchers have provided detailed, critical reviews of the history, experimental approaches, major advances, current ideas and models, as well as future directions, for each of these active areas of research.
Book Synopsis The Eukaryotic RNA Exosome by : John LaCava
Download or read book The Eukaryotic RNA Exosome written by John LaCava and published by Springer Nature. This book was released on 2019-11-25 with total page 514 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume provides a cross-section of RNA exosome research protocols, applied to a diversity of model organisms. Chapters guide readers through methods that e.g. delineate eukaryotic exosomes’ origins in prokaryotes, probe its RNA substrates, adapter complexes and macromolecular interaction of networks, and establish critical structural-function relationships. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, The Eukaryotic RNA Exosome: Methods and Protocols aims to ensure successful results in the further study of this vital field.
Book Synopsis Nuclear Export of Viral RNAs by : J. Hauber
Download or read book Nuclear Export of Viral RNAs written by J. Hauber and published by Springer Science & Business Media. This book was released on 2001-03-27 with total page 148 pages. Available in PDF, EPUB and Kindle. Book excerpt: In eukaryotic cells, the nuclear genome and its transcriptional apparatus is separated from the site of protein synthesis by the nuclear envelope. Thus, a constant flow of proteins and nucleic acids has to cross the nuclear envelope in both directions. This transport in and out of the nucleus is mediated by nuclear pore complexes (NPCs) and occurs in an energy and signal-dependent manner. Thus, nucleocytoplasmic translocation of macro molecules across the nuclear envelope appears to be a highly specific and regulated process. Viruses that replicate their genome in the cell nucleus are therefore forced to develop efficient ways to deal with the intracellulZlr host cell transport machinery. Historically, investigation of Polyomavirus replication allowed identification ofsequences that mediate nuclear import, which led subsequently to our detailed understanding of the cellular factors that are involved in nuclear import. Transport ofmacromolecules in the opposite direction, however, is less well understood. The investigation of retroviral gene expression in recent years pro vided the first insights into the cellular mechanisms that regulate nuclear export. In particular, the detailed dissection of the function of the human immunodeficiency virus type I (HIV-I) Rev trans-activator protein identified CRMI, as a hona fide nuclear export receptor. CRM I appears to be involved in the nucleocytoplasmic translocation of the vast majority of viral and cellular proteins that have subsequently been found to contain a Rev-type leucine-rich nuclear export signal (NES).
Book Synopsis RNA-Protein Interactions : A Practical Approach by : Christopher W.J. Smith
Download or read book RNA-Protein Interactions : A Practical Approach written by Christopher W.J. Smith and published by Oxford University Press, UK. This book was released on 1998-07-09 with total page 370 pages. Available in PDF, EPUB and Kindle. Book excerpt: RNA-protein interactions play a fundamental role in gene expression and protein synthesis. Recent research into the role of RNA in cells has elucidated many more vital interactions with proteins. This book provides an up-to-date and comprehensive guide to a wide range of laboratory procedures to investigate the interactions between RNA and proteins. - ;RNA-protein interactions play a vital role in gene transcription and protein expression. Interactions such as the synthesis of mRNA by RNA polymerases, to the essential modification of RNA by the proteins of the spliceosome complex, and the highly catalytic action of the ribosome in protein synthesis, are established as being fundamental to the function of RNA. Recent research into, for example, the role of RNA as a catalyst, has elucidated many more interactions with proteins that are vital to cell function. RNA - Protein Interactions: A Practical Approach provides a clear and comprehensive guide to the experimental procedures used in studying RNA - protein interactions. The approaches covered range from those initially used to detect a novel RNA-protein interaction, various biochemical and genetic approaches to purifying and cloning RNA binding proteins, through to methods for an in depth analysis of the structural basis of the interaction. The volume includes a number of procedures that have not previously been covered in this type of manual. These include the production of site-specifically modified RNAs by enzymatic and chemical methods and in vivo screening for novel RNA - protein interactions in yeast and E. coli . This is the first volume to gather in one place this wide array of approaches for studying RNA - protein interactions. As is customary for the Practical Approach series, the writing is characterized by a clear explanatory style with many detailed protocols. This informative book will be a valuable aid to laboratory workers in biochemistry and molecular biology - graduate students, postdoctoral and senior scientists - whose research encompasses this field. -
Book Synopsis Characterization of Translation Initiation Factor 1A in Saccharomyces Cerevisiae by : Mami Kainuma
Download or read book Characterization of Translation Initiation Factor 1A in Saccharomyces Cerevisiae written by Mami Kainuma and published by . This book was released on 1996 with total page 326 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis RNA'Protein Interaction Protocols by : Susan R. Haynes
Download or read book RNA'Protein Interaction Protocols written by Susan R. Haynes and published by Springer Science & Business Media. This book was released on 2008-02-03 with total page 485 pages. Available in PDF, EPUB and Kindle. Book excerpt: The molecular characterization of RNA and its interactions with proteins is an important and exciting area of current research. Organisms utilize a variety of RNA–protein interactions to regulate the expression of their genes. This is particularly true for eukaryotes, since newly synthesized messenger RNA must be extensively modified and transported to the cytoplasm before it can be used for protein synthesis. The realization that posttranscriptional processes are critical components of gene regulation has sparked an explosion of interest in both stable ribonucleoprotein (RNP) complexes and transient RNA–protein interactions. RNA is conformationally flexible and can adopt complex structures that provide diverse surfaces for interactions with proteins. The fact that short RNA molecules (aptamers; see Chapter 16) can be selected to bind many different types of molecules is evidence of the structural variability of RNA. RNA molecules are rarely entirely single- or double-stranded, but usually contain multiple short duplexes interrupted by single-stranded loops and bulges; in some RNAs, such as tRNAs, the short duplexes stack on each other. Further variability is generated by the presence of non-Watson-Crick base pairs, modified nucleotides, and more complex structures, such as pseudoknots and triple-strand interactions.
Book Synopsis RNA Binding Proteins by : Zdravko Lorkovic
Download or read book RNA Binding Proteins written by Zdravko Lorkovic and published by CRC Press. This book was released on 2012-08-10 with total page 175 pages. Available in PDF, EPUB and Kindle. Book excerpt: Gene expression in eukaryotes is regulated at different levels, which need to be coordinated to implement the information in the genome. Now it is clear that post-transcriptional regulation of gene expression such as pre-mRNA splicing, mRNA transport, editing, turnover and translation are as important as the control of transcription. In all aspects
Book Synopsis Functional Characterization of Ded1p, a DExD/H Box RNA Helicase, in Saccharomyces Cerevisiae by : Jean-Leon Chong
Download or read book Functional Characterization of Ded1p, a DExD/H Box RNA Helicase, in Saccharomyces Cerevisiae written by Jean-Leon Chong and published by . This book was released on 2004 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: Abstract: The yeast DED1 protein (Ded1p) belongs to an evolutionarily conserved DExD/H-box protein family. Members of this family are involved in essentially all aspects of RNA metabolism, including pre-mRNA splicing, ribosomal biogenesis, RNA transport, and translation. They are often regarded as RNA helicases, or RNA unwindases, because some of them can couple ATP hydrolysis to unwinding short RNA duplexes in vitro. However, recent results suggest that, in vivo, DExD/H box proteins may dissociate specific RNA-binding proteins to remodel ribonucleoprotein complexes. Our lab previously found that Ded1p is a novel essential translation factor. However, Ded1p's mechanistic role in translation remains to be defined. To examine Ded1p's role further, I sought to identify its interacting proteins. In the first part of this dissertation (Chapter 1 to 4), I describe the unexpectedly finding that Ded1p binds to yeast L-A virus particles and accelerates the rate of L-A's negative-strand RNA synthesis in vitro. Viruses are intracellular parasites that must use the host machinery to multiply, because their genomes are very small. Thus, identification of the host factors that perform essential functions in viral replication is of crucial importance to the understanding of virus-host interactions. The findings in this dissertation and the fact that Ded1p is also required for translating the brome mosaic virus RNA2 in yeast thus raise an intriguing possibility that Ded1p is one of the key host factors favored by several evolutionarily related RNA viruses including the human hepatitis C virus. In the second part of this dissertation (Chapter 5), I show that Ded1p is associated with active spliceosomes and inactivation of Ded1p affects spliceosome formation. Moreover, data from our lab demonstrates that introns accumulate in ded1 mutant strains after being shifted to non-permissive temperature. These findings indicate that Ded1p is also involved in pre-mRNA splicing. A growing body of evidence has suggested that many proteins can function in different nuclear processes in gene expression, implying a close inter-dependent relationship among these processes. The observations in this dissertation and the fact that Ded1p is a translation factor suggest a possible intimate coordination in gene expression between the nucleus and the cytoplasm.
Book Synopsis Role of Protein-Protein Interactions in Metabolism: Genetics, Structure, Function, 2nd Edition by : Amit V. Pandey
Download or read book Role of Protein-Protein Interactions in Metabolism: Genetics, Structure, Function, 2nd Edition written by Amit V. Pandey and published by Frontiers Media SA. This book was released on 2018-06-20 with total page 152 pages. Available in PDF, EPUB and Kindle. Book excerpt: Genetic variations may change the structure and function of individual proteins as well as affect their interactions with other proteins and thereby impact metabolic processes dependent on protein-protein interactions. For example, cytochrome P450 proteins, which metabolize a vast array of drugs, steroids and other xenobiotics, are dependent on interactions with redox and allosteric partner proteins for their localization, stability, (catalytic) function and metabolic diversity (reactions). Genetic variations may impact such interactions by changing the splicing and/or amino acid sequence which in turn may impact protein topology, localization, post translational modifications and three dimensional structure. More generally, research on single gene defects and their role in disease, as well as recent large scale sequencing studies suggest that a large number of genetic variations may contribute to disease not only by affecting gene function or expression but also by modulating complex protein interaction networks. The aim of this research topic is to bring together researchers working in the area of drug, steroid and xenobiotic metabolism who are studying protein-protein interactions, to describe their recent advances in the field. We are aiming for a comprehensive analysis of the subject from different approaches including genetics, proteomics, transcriptomics, structural biology, biochemistry and pharmacology. Of particular interest are papers dealing with translational research describing the role of novel genetic variations altering protein-protein interaction. Authors may submit original articles, reviews and opinion or hypothesis papers dealing with the role of protein-protein interactions in health and disease. Potential topics include, but are not limited to: • Role of protein-protein interactions in xenobiotic metabolism by cytochrome P450s and other drug metabolism enzymes. • Role of classical and novel interaction partners for cytochrome P450-dependent metabolism which may include interactions with redox partners, interactions with other P450 enzymes to form P450 dimers/multimers, P450-UGT interactions and proteins involved in posttranslational modification of P450s. • Effect of genetic variations (mutations and polymorphisms) on metabolism affected by protein-protein interactions. • Structural implications of mutations and polymorphisms on protein-protein interactions. • Functional characterization of protein-protein interactions. • Analysis of protein-protein interaction networks in health and disease. • Regulatory mechanisms governing metabolic processes based on protein-protein interactions. • Experimental approaches for identification of new protein-protein interactions including changes caused by mutations and polymorphisms.
Book Synopsis RNA Binding Proteins in Development and Disease by : Robert B. Denman
Download or read book RNA Binding Proteins in Development and Disease written by Robert B. Denman and published by . This book was released on 2008-01-01 with total page 214 pages. Available in PDF, EPUB and Kindle. Book excerpt: Introduction - The circle of life is a philosophical construct, for want of a better term that posits the interconnectedness of all living things. In the movie The Lion King which brought the term to the consciousness of the Western mind, Mufasa tries to explain the concept to his heir-apparent, Simba, by saying, we eat the antelope, but when we die, we become the grass that they eat . While the circle of life has an uncanny resonance with something deep within, especially for children, being Disney marketing magic it is never spelled out what actually connects us. I would submit to you that what this book demonstrates is that RNA binding proteins (RBPs) are some of the stuff connecting the circle of life. They are ageless, they are ubiquitous, and they are diverse. Found in all kingdoms, phyla and subphyla they impinge on all aspects of cellular metabolism from the transcription and splicing of messenger RNAs, to the formation of ribosomal RNA (see Aveilla et al. Chapter 11), from the translation of messenger RNAs (see Zhong et al. Chapter 1), to the timing of their translation and their degradation (see Pelletier et al. Chapter 2 and Vessey et al. Chapter 5). They are chaperones and repressors. More broadly they are regulators, which are in turn are regulated by a host of post-translational modifications (see Gopal et al. Chapter 3 and Kobarg Chapter 4). They play important roles in the differentiation of the nervous system (see Kawahara et al. Chapter 6), in muscle development (see Denman Chapter 7) and in germ line development (see Vessey et al. Chapter 5). Correspondingly, as befits a central role in metabolism, their loss or mutation can have profound organismal affects, from outright lethality to cancer (see Noubissi et al. Chapter 8), muscular disorders (see Meola et al. Chapter 9), eye disorders (see Schreuder et al. Chapter 10), bone marrow failure (see Aveilla et al. Chapter 11) epilepsy and mental retardation (Zhong et al. Chapter 1). Quite simply, they are RNA binding proteins . I would therefore invite the interested reader on an abbreviated tour of the circle of life that is contained in this slender tome. Of course, the very enterprise of making a book places constraints on what is included and what is not. As there are currently more than 30,000 papers on PubMed dealing with RNA binding proteins it is probable that many a would-be-reader will not find his or her favorite RBP reviewed here. Nevertheless, I would commend this book as a representative sampling of those articles, and therefore a worthwhile read for several reasons. First, the modular architecture of RBPs produces diversity via the mixing of a limited set of RNA binding motifs. Doubtless, one of the motifs the would-be reader is familiar with is discussed in these pages. Therefore, this book would offer a unique view of this motif operating in a different context. Second, the most common problem attended to within the field of RNA binding proteins is, What RNAs bind to a particular RBP and how do we know they are bona fide? By reading this book you will see how others have dealt with, or are dealing with this problem providing you the reader with the insight needed to attack this problem on another front. Finally, the dynamism of RBP-RNA interactions, coupled with their propensity to form larger aggregates make it likely that the RBP most familiar to a would-be reader associates with one or more of the RBPs reviewed here. If so, I would say, in order to know thyself, know thy neighbor . It is after all the circle of life.
