Cell Culture Techniques In Heart And Vessel Research

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Cell Culture Techniques in Heart and Vessel Research

Author : H.M. Piper
Publisher : Springer Science & Business Media
ISBN 13 : 3642752624
Total Pages : 568 pages
Book Rating : 4.6/5 (427 download)

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Book Synopsis Cell Culture Techniques in Heart and Vessel Research by : H.M. Piper

Download or read book Cell Culture Techniques in Heart and Vessel Research written by H.M. Piper and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 568 pages. Available in PDF, EPUB and Kindle. Book excerpt: In the cardiovascular sciences, an increasing demand for the use of modern methods of cell biology has developed. The use of specific cell culture models of the various tissues involved is essential for most of these novel approaches. This book meets the demand for acomprehensive and easy accessible source for cell cul- ture methods in cardiovascular research as it was not previously available. The basic methods for cultures of cardiomyocytes (embryonic and adult), endothelial cells (micro- and macrovascular), smooth muscle cells and pericytes are described in detail by an international selection of experts. Special chapters discuss the use of growth factors and attachment substrates, techniques for co-cultures, cultures on permeable filter membranes and microcarrier cultures. The methodological descriptions are sufficiently detailed for an immediate application in the laboratory. All chapters also contain a critical evaluation of alternative approaches.

Methods in Endothelial Cell Biology

Author : Hellmut G. Augustin
Publisher : Springer Science & Business Media
ISBN 13 : 3642187250
Total Pages : 381 pages
Book Rating : 4.6/5 (421 download)

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Book Synopsis Methods in Endothelial Cell Biology by : Hellmut G. Augustin

Download or read book Methods in Endothelial Cell Biology written by Hellmut G. Augustin and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 381 pages. Available in PDF, EPUB and Kindle. Book excerpt: Endothelial cell biology has developed into a vibrant discipline and has become a critical instrument to study several disease processes on the cellular and molecular level. It is now widely recognized that dysfunctions of normal endothelial cell homeostasis are involved in some of the most important human diseases, including ischemic heart diseases, hypertension, atherosclerosis, tumors, diabetes, arthritis, and inflammation. Further, the increasing importance and recognition of the field of vascular biology in general requires in vitro and in vivo techniques in order to address the complex questions. Methods in Endothelial Cell Biology is a comprehensive practical "how-to"-guide summarizing the most relevant established techniques as well as a number of new emerging techniques. Easy-to-follow reliable protocols provide a useful lab bench resource for the experienced researcher and newcomer to the field.

Endothelial Cell Culture

Author : Roy Bicknell
Publisher : Cambridge University Press
ISBN 13 : 9780521559904
Total Pages : 156 pages
Book Rating : 4.5/5 (599 download)

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Book Synopsis Endothelial Cell Culture by : Roy Bicknell

Download or read book Endothelial Cell Culture written by Roy Bicknell and published by Cambridge University Press. This book was released on 1996-09-28 with total page 156 pages. Available in PDF, EPUB and Kindle. Book excerpt: The aim of the Handbooks in Practical Animal Cell Biology is to provide practical workbooks for those involved in primary cell culture. Each volume addresses a different cell lineage, and contains an introductory section followed by individual chapters on the culture of specific differentiated cell types. The authors of each chapter are leading researchers in their fields and use their first-hand experience to present reliable techniques in a clear and thorough manner. Endothelial Cell Culture contains chapters on endothelial cells derived from 1) lung, 2) bone marrow, 3) brain, 4) mammary glands, 5) skin, 6) adipose tissue, 7) female reproductive system, and 8) synovium.

Practical Methods in Cardiovascular Research

Author : Stefan Dhein
Publisher : Springer Science & Business Media
ISBN 13 : 9783540407638
Total Pages : 1036 pages
Book Rating : 4.4/5 (76 download)

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Book Synopsis Practical Methods in Cardiovascular Research by : Stefan Dhein

Download or read book Practical Methods in Cardiovascular Research written by Stefan Dhein and published by Springer Science & Business Media. This book was released on 2005-03-17 with total page 1036 pages. Available in PDF, EPUB and Kindle. Book excerpt: Scientists working or planning to work in the field of cardiovascular research will welcome Practical Methods in Cardiovascular Research as the reference book they have long been waiting for. Not only general aspects of cardiovascular research are well presented, but also detailed descriptions of methods and protocols and practical examples. Written by leading scientists in their field, chapters cover classical methods such as the Langendorff heart or working heart models as well as numerous new techniques and methods. Newcomers and experienced researchers alike will benefit from the troubleshooting guide in each chapter, the extensive reference lists for advanced reading and the great practical experience of the authors. Practical Methods in Cardiovascular Research is therefore a long awaited "must have" for anybody with an interest in cardiovascular research.

Cardiac Cell Culture Technologies

Author : Zbigniew Brzozka
Publisher : Springer
ISBN 13 : 9783319889795
Total Pages : 0 pages
Book Rating : 4.8/5 (897 download)

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Book Synopsis Cardiac Cell Culture Technologies by : Zbigniew Brzozka

Download or read book Cardiac Cell Culture Technologies written by Zbigniew Brzozka and published by Springer. This book was released on 2018-09-04 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book provides an introduction to the biological background of heart functioning and analyzes the various materials and technologies used for the development of microfluidic systems dedicated to cell culture, with an emphasis on cardiac cells. The authors describe the characterization of microfluidic systems for cardiac cell culture and center their discussion of the use of stem cell stimulation based on four different types: electrical, biochemical, physical, and mechanical. This book is appropriate for researchers focused on on-chip technologies and heart studies, students in bioengineering and microengineering courses, and a variety of professionals, such as biotechnologists, biomedical engineers, and clinicians working in the cardiac diseases field.

Practical Methods in Cardiovascular Research

Author : Stefan Dhein
Publisher : Springer
ISBN 13 : 3540265740
Total Pages : 1011 pages
Book Rating : 4.5/5 (42 download)

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Book Synopsis Practical Methods in Cardiovascular Research by : Stefan Dhein

Download or read book Practical Methods in Cardiovascular Research written by Stefan Dhein and published by Springer. This book was released on 2006-02-28 with total page 1011 pages. Available in PDF, EPUB and Kindle. Book excerpt: Scientists working or planning to work in the field of cardiovascular research will welcome Methods in Cardiovascular Research as the reference book they have been waiting for. Not only general aspects of cardiovascular research are well presented but also detailed descriptions of methods, protocols and practical examples. Written by leading scientists in their field, chapters cover classical methods such as the Langendorff heart or working heart models as well as numerous new techniques and methods. Newcomers and experienced researchers alike will benefit from the troubleshooting guide in each chapter, the extensive reference lists for advanced reading and the great practical experience of the authors. Methods in Cardiovascular Research is a "must have" for anybody with an interest in cardiovascular research.

Biology of Endothelial Cells

Author : Eric A. Jaffe
Publisher : Springer Science & Business Media
ISBN 13 : 146132825X
Total Pages : 471 pages
Book Rating : 4.4/5 (613 download)

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Book Synopsis Biology of Endothelial Cells by : Eric A. Jaffe

Download or read book Biology of Endothelial Cells written by Eric A. Jaffe and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 471 pages. Available in PDF, EPUB and Kindle. Book excerpt: The participation of endothelial cells in various physiologic and pathologic processes has been hypothesized since before the turn of the century. However, until recently, direct evidence for endothelial involvement in these processes has been extremely difficult to obtain due to the inability to study endothelial cell function in vitro. Though the possibility of using cultured endothelial cells to study endothelial cell function in vitro was recognized many years ago, the inability to culture unambiguously identifiable endothelial cells limited investigators in their studies of endothelial function. As a result, the field of endothelial cell biology lay relatively fallow for many years. The development in the early 1970's of routine and easily implemented methods for culturing human endothelial cells and the demonstration that cultured endothelial cells synthesized a physiologically relevant protein, Factor VIII/von Willebrand Factor, quickly changed this state of affairs. Over the following decade the scope of endothelial cell research rapidly widened, spreading in a number of directions. First, methods were developed to culture endothelial cells from a variety of species. Second, methods were developed to culture endothelial cells from different organs and types of blood vessels (arteries, veins, and capillaries) within a single species. Third, and most important, investigators began using cultured endothelial cells as tools to study the potential involvement of endothelial cells in a wide assortment of biologically interesting processes. The net result has been a tremendous increase in our understanding of endothelial cell function.

Post-Genomic Cardiology

Author : José Marín-García
Publisher : Academic Press
ISBN 13 : 0124046428
Total Pages : 935 pages
Book Rating : 4.1/5 (24 download)

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Book Synopsis Post-Genomic Cardiology by : José Marín-García

Download or read book Post-Genomic Cardiology written by José Marín-García and published by Academic Press. This book was released on 2014-05-09 with total page 935 pages. Available in PDF, EPUB and Kindle. Book excerpt: In this second edition of Post-Genomic Cardiology, developing and new technologies such as translational genomics, next generation sequencing (NGS), bioinformatics, and systems biology in molecular cardiology are assessed in light of their therapeutic potential. As new methods of mutation screening emerge, both for the genome and for the “epigenome, comprehensive understanding of the many mutations that underlie cardiovascular diseases and adverse drug reactions is within our reach. This book, written by respected cardiologist José Marín-García, features discussion on the Hap-Map: the largest international effort to date aiming to define the differences between our individual genomes. This unique reference further reviews and investigates genome sequences from our evolutionary relatives that could help us decipher the signals of genes, and offers a comprehensive and critical evaluation of regulatory elements from the complicated network of the background DNA. Offers updated discussion of cutting-edge molecular techniques including new genomic sequencing / NGS / Hap-Map / bioinformatics / systems biology approaches Analyzes mitochondria dynamics and their role in cardiac dysfunction, up-to-date analysis of cardio-protection, and cardio-metabolic syndrome Presents recent translational studies, gene therapy, transplantation of stem cells, and pharmacological treatments in CVDs

Introduction to Cell and Tissue Culture

Author : Jennie P. Mather
Publisher : Springer Science & Business Media
ISBN 13 : 0585275718
Total Pages : 247 pages
Book Rating : 4.5/5 (852 download)

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Book Synopsis Introduction to Cell and Tissue Culture by : Jennie P. Mather

Download or read book Introduction to Cell and Tissue Culture written by Jennie P. Mather and published by Springer Science & Business Media. This book was released on 2007-08-20 with total page 247 pages. Available in PDF, EPUB and Kindle. Book excerpt: It is a pleasure to contribute the foreword to Introduction to Cell and Tissue Culture: The ory and Techniques by Mather and Roberts. Despite the occasional appearance of thought ful works devoted to elementary or advanced cell culture methodology, a place remains for a comprehensive and definitive volume that can be used to advantage by both the novice and the expert in the field. In this book, Mather and Roberts present the relevant method ology within a conceptual framework of cell biology, genetics, nutrition, endocrinology, and physiology that renders technical cell culture information in a comprehensive, logical for mat. This allows topics to be presented with an emphasis on troubleshooting problems from a basis of understanding the underlying theory. The material is presented in a way that is adaptable to student use in formal courses; it also should be functional when used on a daily basis by professional cell culturists in a- demia and industry. The volume includes references to relevant Internet sites and other use ful sources of information. In addition to the fundamentals, attention is also given to mod ern applications and approaches to cell culture derivation, medium formulation, culture scale-up, and biotechnology, presented by scientists who are pioneers in these areas. With this volume, it should be possible to establish and maintain a cell culture laboratory devot ed to any of the many disciplines to which cell culture methodology is applicable.

Molecular Biology of the Cell

Author :
Publisher :
ISBN 13 : 9780815332183
Total Pages : 0 pages
Book Rating : 4.3/5 (321 download)

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Book Synopsis Molecular Biology of the Cell by :

Download or read book Molecular Biology of the Cell written by and published by . This book was released on 2002 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Angiogenesis Assays

Author : Carolyn A. Staton
Publisher : John Wiley & Sons
ISBN 13 : 047002934X
Total Pages : 410 pages
Book Rating : 4.4/5 (7 download)

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Book Synopsis Angiogenesis Assays by : Carolyn A. Staton

Download or read book Angiogenesis Assays written by Carolyn A. Staton and published by John Wiley & Sons. This book was released on 2007-01-11 with total page 410 pages. Available in PDF, EPUB and Kindle. Book excerpt: Angiogenesis, the development of new blood vessels from the existing vasculature, is essential for physiological growth and over 18,000 research articles have been published describing the role of angiogenesis in over 70 different diseases, including cancer, diabetic retinopathy, rheumatoid arthritis and psoriasis. One of the most important technical challenges in such studies has been finding suitable methods for assessing the effects of regulators of eh angiogenic response. While increasing numbers of angiogenesis assays are being described both in vitro and in vivo, it is often still necessary to use a combination of assays to identify the cellular and molecular events in angiogenesis and the full range of effects of a given test protein. Although the endothelial cell - its migration, proliferation, differentiation and structural rearrangement - is central to the angiogenic process, it is not the only cell type involved. the supporting cells, the extracellular matrix and the circulating blood with its cellular and humoral components also contribute. In this book, experts in the use of a diverse range of assays outline key components of these and give a critical appraisal of their strengths and weaknesses. Examples include assays for the proliferation, migration and differentiation of endothelial cells in vitro, vessel outgrowth from organ cultures, assessment of endothelial and mural cell interactions, and such in vivo assays as the chick chorioallantoic membrane, zebrafish, corneal, chamber and tumour angiogenesis models. These are followed by a critical analysis of the biological end-points currently being used in clinical trials to assess the clinical efficacy of anti-angiogenic drugs, which leads into a discussion of the direction future studies should take. This valuable book is of interest to research scientists currently working on angiogenesis in both the academic community and in the biotechnology and pharmaceutical industries. Relevant disciplines include cell and molecular biology, oncology, cardiovascular research, biotechnology, pharmacology, pathology and physiology.

Culture of Cells for Tissue Engineering

Author : Gordana Vunjak-Novakovic
Publisher : John Wiley & Sons
ISBN 13 : 0471741809
Total Pages : 528 pages
Book Rating : 4.4/5 (717 download)

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Book Synopsis Culture of Cells for Tissue Engineering by : Gordana Vunjak-Novakovic

Download or read book Culture of Cells for Tissue Engineering written by Gordana Vunjak-Novakovic and published by John Wiley & Sons. This book was released on 2006-03-31 with total page 528 pages. Available in PDF, EPUB and Kindle. Book excerpt: Step-by-step, practical guidance for the acquisition, manipulation,and use of cell sources for tissue engineering Tissue engineering is a multidisciplinary field incorporatingthe principles of biology, chemistry, engineering, and medicine tocreate biological substitutes of native tissues for scientificresearch or clinical use. Specific applications of this technologyinclude studies of tissue development and function, investigatingdrug response, and tissue repair and replacement. This area israpidly becoming one of the most promising treatment options forpatients suffering from tissue failure. Written by leading experts in the field, Culture of Cellsfor Tissue Engineering offers step-by-step, practicalguidance for the acquisition, manipulation, and use of cell sourcesfor tissue engineering. It offers a unique focus on tissueengineering methods for cell sourcing and utilization, combiningtheoretical overviews and detailed procedures. Features of the text include: Easy-to-use format with a two-part organization Logically organized—part one discusses cell sourcing,preparation, and characterization and the second part examinesspecific engineered tissues Each chapter covers: structural and functional properties oftissues, methodological principles, culture, cellselection/expansion, cell modifications, cell seeding, tissueculture, analytical assays, and a detailed description ofrepresentative studies End-of-chapter features include useful listings of sources forreagents, materials, and supplies, with the contact details of thesuppliers listed at the end of the book A section of elegant color plates to back up the figures in thechapters Culture of Cells for Tissue Engineering givesnovice and seasoned researchers in tissue engineering an invaluableresource. In addition, the text is suitable for professionals inrelated research, particularly in those areas where cell and tissueculture is a new or emerging tool.

Cells and Tissues in Culture Methods, Biology and Physiology

Author : E. N. Willmer
Publisher : Elsevier
ISBN 13 : 1483277232
Total Pages : 843 pages
Book Rating : 4.4/5 (832 download)

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Book Synopsis Cells and Tissues in Culture Methods, Biology and Physiology by : E. N. Willmer

Download or read book Cells and Tissues in Culture Methods, Biology and Physiology written by E. N. Willmer and published by Elsevier. This book was released on 2013-10-02 with total page 843 pages. Available in PDF, EPUB and Kindle. Book excerpt: Cells and Tissues in Culture: Methods, Biology, and Physiology, Volume 3 focuses on the applications of the methods of tissue culture to various fields of investigation, including virology, immunology, and preventive medicine. The selection first offers information on molecular organization of cells and tissues in culture and tissue culture in radiobiology. Topics include cellular organization at the molecular level, fibrogenesis in tissue culture, effect of radiation on the growth of isolated cells, and irradiation of the selected parts of the cell. The publication then considers the effects of invading organisms on cells and tissues in culture and cell, tissue, and organ cultures in virus research. The book elaborates on antibody production in tissue culture and tissue culture in pharmacology. Discussions focus on early attempts at in vitro studies, tissue culture in the study of pharmacologically active agents, and methods of assessment of drug activity. The text also reviews invertebrate tissue and organ culture in cell research; introduction and methods employed in plant tissue culture; and growth, differentiation and organogenesis in plant tissue and organ cultures. The selection is a vital source of data for readers interested in the culture of cells and tissues.

In Vitro Methods in Pharmaceutical Research

Author : Jose V. Castell
Publisher : Elsevier
ISBN 13 : 0080534600
Total Pages : 483 pages
Book Rating : 4.0/5 (85 download)

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Book Synopsis In Vitro Methods in Pharmaceutical Research by : Jose V. Castell

Download or read book In Vitro Methods in Pharmaceutical Research written by Jose V. Castell and published by Elsevier. This book was released on 1996-10-04 with total page 483 pages. Available in PDF, EPUB and Kindle. Book excerpt: In Vitro Methods in Pharmaceutical Research provides a comprehensive guide to laboratory techniques for evaluating in vitro organ toxicity using cellular models. Step-by-step practical tips on how to perform and interpret assays for drug metabolism and toxicity assessment are provided, along with a comparison of different techniques available. It is a welcome addition to the literature at a time when interest is growing in cellular in vitro models for toxicology and pharmacology studies. Meets the continuing demand for information in this field Compares In Vitro techniques with other methods Describes cell-culture methods used to investigate toxicity in cells derived from different organs Includes contributions by leading experts in the field

Culturing Life

Author : Hannah Landecker
Publisher : Harvard University Press
ISBN 13 : 9780674023284
Total Pages : 302 pages
Book Rating : 4.0/5 (232 download)

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Book Synopsis Culturing Life by : Hannah Landecker

Download or read book Culturing Life written by Hannah Landecker and published by Harvard University Press. This book was released on 2010-03-30 with total page 302 pages. Available in PDF, EPUB and Kindle. Book excerpt: How did cells make the journey, one we take so much for granted, from their origin in living bodies to something that can be grown and manipulated on artificial media in the laboratory, a substantial biomass living outside a human body, plant, or animal? This is the question at the heart of Hannah Landecker's book. She shows how cell culture changed the way we think about such central questions of the human condition as individuality, hybridity, and even immortality and asks what it means that we can remove cells from the spatial and temporal constraints of the body and "harness them to human intention." Rather than focus on single discrete biotechnologies and their stories--embryonic stem cells, transgenic animals--Landecker documents and explores the wider genre of technique behind artificial forms of cellular life. She traces the lab culture common to all those stories, asking where it came from and what it means to our understanding of life, technology, and the increasingly blurry boundary between them. The technical culture of cells has transformed the meaning of the term "biological," as life becomes disembodied, distributed widely in space and time. Once we have a more specific grasp on how altering biology changes what it is to be biological, Landecker argues, we may be more prepared to answer the social questions that biotechnology is raising.

Basic Cell Culture Protocols

Author : Cheryl D. Helgason
Publisher : Humana Press
ISBN 13 : 9781627031271
Total Pages : 550 pages
Book Rating : 4.0/5 (312 download)

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Book Synopsis Basic Cell Culture Protocols by : Cheryl D. Helgason

Download or read book Basic Cell Culture Protocols written by Cheryl D. Helgason and published by Humana Press. This book was released on 2012-11-20 with total page 550 pages. Available in PDF, EPUB and Kindle. Book excerpt: At some point in their careers, virtually every scientist and technician, as well as many medical professionals, regardless of their area of specialization have a need to utilize cell culture systems. Updating and significantly expanding upon the previous editions, Basic Cell Culture Protocols, Fourth Edition provides the novice cell culturist with sufficient information to perform the basic techniques, to ensure the health and identity of their cell lines, and to be able to isolate and culture specialized primary cell types. The intent of this extensive volume is to generate a valuable resource containing clear methodologies pertinent to current areas of investigation, rather than attempting to educate cell culturists on specific cell types or organ systems. Written in the highly successful Methods in Molecular BiologyTM, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and up-to-date, Basic Cell Culture Protocols, Fourth Edition compiles the essential techniques needed to approach this vital laboratory activity with full success.

Stem Cells

Author : Christine L. Mummery
Publisher : Academic Press
ISBN 13 : 0124115675
Total Pages : 446 pages
Book Rating : 4.1/5 (241 download)

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Book Synopsis Stem Cells by : Christine L. Mummery

Download or read book Stem Cells written by Christine L. Mummery and published by Academic Press. This book was released on 2014-05-23 with total page 446 pages. Available in PDF, EPUB and Kindle. Book excerpt: The second edition of Stem Cells: Scientific Facts and Fiction provides the non-stem cell expert with an understandable review of the history, current state of affairs, and facts and fiction of the promises of stem cells. Building on success of its award-winning preceding edition, the second edition features new chapters on embryonic and iPS cells and stem cells in veterinary science and medicine. It contains major revisions on cancer stem cells to include new culture models, additional interviews with leaders in progenitor cells, engineered eye tissue, and xeno organs from stem cells, as well as new information on "organs on chips" and adult progenitor cells. In the past decades our understanding of stem cell biology has increased tremendously. Many types of stem cells have been discovered in tissues that everyone presumed were unable to regenerate in adults, the heart and the brain in particular. There is vast interest in stem cells from biologists and clinicians who see the potential for regenerative medicine and future treatments for chronic diseases like Parkinson's, diabetes, and spinal cord lesions, based on the use of stem cells; and from entrepreneurs in biotechnology who expect new commercial applications ranging from drug discovery to transplantation therapies. Explains in straightforward, non-specialist language the basic biology of stem cells and their applications in modern medicine and future therapy Includes extensive coverage of adult and embryonic stem cells both historically and in contemporary practice Richly illustrated to assist in understanding how research is done and the current hurdles to clinical practice