Carbon-13 NMR Spectroscopy of Biological Systems

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Publisher : Elsevier
ISBN 13 : 0080528554
Total Pages : 355 pages
Book Rating : 4.0/5 (85 download)

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Book Synopsis Carbon-13 NMR Spectroscopy of Biological Systems by : Nicolau Beckmann

Download or read book Carbon-13 NMR Spectroscopy of Biological Systems written by Nicolau Beckmann and published by Elsevier. This book was released on 1995-04-06 with total page 355 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book is intended to provide an in-depth understanding of 13C NMR as a tool in biological research. 13C NMR has provided unique information concerning complex biological systems, from proteins and nucleic acids to animals and humans. The subjects addressed include multidimensional heteronuclear techniques for structural studies of molecules in the liquid and solid states, the investigation of interactions in model membranes, the elucidation of metabolic pathwaysin vitro and in vivo on animals, and noninvasive metabolic studies performed on humans. The book is a unique mix of NMR methods and biological applications which makes it a convenient reference for those interested in research in this interdisciplinary area of physics, chemistry, biology, and medicine. An interdisciplinary text with emphasis on both 13C NMR methodology and the relevant biological and biomedical issues State-of-the-art 13C NMR techniques are described; Whenever possible, their advantages over other approaches are emphasized The chapters constitute comprehensive reviews and are written by acknowledged experts in their fields Chapters are written in a clear style, and include a large number of illustrations and comprehensive references

Recent Applications of 13C NMR Spectroscopy to Biological Systems

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Publisher :
ISBN 13 :
Total Pages : pages
Book Rating : 4.:/5 (727 download)

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Book Synopsis Recent Applications of 13C NMR Spectroscopy to Biological Systems by :

Download or read book Recent Applications of 13C NMR Spectroscopy to Biological Systems written by and published by . This book was released on 1981 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: Carbon-13 nuclear magnetic resonance (NMR) spectroscopy, in conjunction with carbon-13 labelling, is a powerful new analytical technique for the study of metabolic pathways and structural components in intact organelles, cells, and tissues. The technique can provide, rapidly and non-destructively, unique information about: the architecture and dynamics of structural components; the nature of the intracellular environment; and metabolic pathways and relative fluxes of individual carbon atoms. With the aid of results recently obtained by us and those reported by a number of other laboratories, the problems and potentialities of the technique will be reviewed with emphasis on: the viscosities of intracellular fluids; the structure and dynamics of the components of membranes; and the primary and secondary metabolic pathways of carbon in microorganisms, plants, and mammalian cells in culture.

Carbon 13 NMR Spectroscopy

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Author :
Publisher : John Wiley & Sons
ISBN 13 :
Total Pages : 798 pages
Book Rating : 4.3/5 (91 download)

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Book Synopsis Carbon 13 NMR Spectroscopy by : Hans-Otto Kalinowski

Download or read book Carbon 13 NMR Spectroscopy written by Hans-Otto Kalinowski and published by John Wiley & Sons. This book was released on 1988 with total page 798 pages. Available in PDF, EPUB and Kindle. Book excerpt: 13C NMR spectroscopy has not only become an established and well documented technique, but is about to yield even more detailed information on increasingly complex organic and biological systems, through the possibilities opened by pulse techniques. This work describes these techniques.

Biological NMR Spectroscopy

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Author :
Publisher : Oxford University Press
ISBN 13 : 0195357426
Total Pages : 375 pages
Book Rating : 4.1/5 (953 download)

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Book Synopsis Biological NMR Spectroscopy by : John L. Markley

Download or read book Biological NMR Spectroscopy written by John L. Markley and published by Oxford University Press. This book was released on 1997-01-30 with total page 375 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book presents a critical assessment of progress on the use of nuclear magnetic resonance spectroscopy to determine the structure of proteins, including brief reviews of the history of the field along with coverage of current clinical and in vivo applications. The book, in honor of Oleg Jardetsky, one of the pioneers of the field, is edited by two of the most highly respected investigators using NMR, and features contributions by most of the leading workers in the field. It will be valued as a landmark publication that presents the state-of-the-art perspectives regarding one of today's most important technologies.

Biological Magnetic Resonance

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Author :
Publisher : Springer Science & Business Media
ISBN 13 : 0306470780
Total Pages : 265 pages
Book Rating : 4.3/5 (64 download)

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Book Synopsis Biological Magnetic Resonance by : Lawrence J. Berliner

Download or read book Biological Magnetic Resonance written by Lawrence J. Berliner and published by Springer Science & Business Media. This book was released on 2006-04-11 with total page 265 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume constitutes a compilation of the latest experiments and theories on a rapidly evolving and maturing field in MRI/MRS, which is the use of the stable isotope 13-C. The 13-C is used to probe the chemistry, mechanism, and function in living systems. All the chapters are written by experts in the field who discuss topics such as `Tracer Theory and the Suitability of 13-C NMR', `Applications of 13-C to Studies of Human Brain Metabolism', etc.

Nuclear magnetic Resonance in biochemistry

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Publisher : Elsevier
ISBN 13 : 0323141048
Total Pages : 428 pages
Book Rating : 4.3/5 (231 download)

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Book Synopsis Nuclear magnetic Resonance in biochemistry by : Thomas James

Download or read book Nuclear magnetic Resonance in biochemistry written by Thomas James and published by Elsevier. This book was released on 2012-12-02 with total page 428 pages. Available in PDF, EPUB and Kindle. Book excerpt: Nuclear Magnetic Resonance in Biochemistry: Principles and Applications focuses on the principles and applications of nuclear magnetic resonance (NMR) in biochemistry. Topics covered include experimental methods in NMR; the mechanisms of NMR relaxation; chemical and paramagnetic shifts; spin-spin splitting; the use of NMR in investigations of biopolymers and biomolecular interactions; and molecular dynamics in biological and biochemical systems. This text is comprised of eight chapters; the first of which gives an overview of NMR spectroscopy and its use in studies of biological systems. The next two chapters discuss the theoretical basis for NMR applications in biochemistry, with emphasis on Bloch equations, quantum mechanics, correlation function and correlation time, double resonance, and chemical exchange. The reader is then introduced to the basis for chemical shifts and spin-spin splitting, along with several examples of the use of these NMR parameters in studies of small molecule interactions and structure. The experimental apparatus and procedures employed in NMR studies, Fourier transform NMR, and NMR spectral parameters of small molecules interacting with macromolecules are also considered. The book highlights the information obtainable from the spectra of biopolymers, and then concludes with a chapter on NMR investigations of the state of motion of lipids in membranes and model membranes; water in macromolecular and cellular systems; and sodium ion in biological tissue. This book is intended primarily for chemists, biochemists, biophysicists, and molecular biologists, as well as graduate students.

Biological Magnetic Resonance

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Publisher : Springer Science & Business Media
ISBN 13 : 1461565375
Total Pages : 361 pages
Book Rating : 4.4/5 (615 download)

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Book Synopsis Biological Magnetic Resonance by : Lawrence J. Berliner

Download or read book Biological Magnetic Resonance written by Lawrence J. Berliner and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 361 pages. Available in PDF, EPUB and Kindle. Book excerpt: We are pleased to present this second volume of a series that has already received much interest. The application of magnetic resonance methods to the study of actual biological systems as contrasted to cell-free samples, although not entirely novel, as demonstrated by Civan and Shporer in Volume I, has taken on new dimensions with the use of phosphorus-31 and carbon-13 NMR in studying cells, tissues, and organelles. The applications of 31 P NMR to such systems is reviewed in this volume, while carbon-13 will be covered in a later one. The use of nitroxide spin labels has grown to the point where it now may be considered a common biological technique. The synthesis and applications of a new class of nitroxides is described in this volume. ESR spectroscopy of paramagnetic ions is a powerful approach to studying molecular and structural details, as the chapter by Boas, Pilbrow, and Smith on the ESR of copper in Volume 1 has shown. In this volume the ESR of molybdenum and iron is treated in a comparable fashion. In the first volume some aspects of 1 H NMR spectroscopy of certain classes of In this volume the high-resolu biological macromolecules were discussed.· tion multinuclear NMR spectra of peptides, including the physiologically significant peptide hormones, are reviewed.

Methodologies for Metabolomics

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Publisher : Cambridge University Press
ISBN 13 : 0521765900
Total Pages : 641 pages
Book Rating : 4.5/5 (217 download)

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Book Synopsis Methodologies for Metabolomics by : Norbert W. Lutz

Download or read book Methodologies for Metabolomics written by Norbert W. Lutz and published by Cambridge University Press. This book was released on 2013-01-21 with total page 641 pages. Available in PDF, EPUB and Kindle. Book excerpt: Metabolomics, the global characterisation of the small molecule complement involved in metabolism, has evolved into a powerful suite of approaches for understanding the global physiological and pathological processes occurring in biological organisms. The diversity of metabolites, the wide range of metabolic pathways and their divergent biological contexts require a range of methodological strategies and techniques. Methodologies for Metabolomics provides a comprehensive description of the newest methodological approaches in metabolomic research. The most important technologies used to identify and quantify metabolites, including nuclear magnetic resonance and mass spectrometry, are highlighted. The integration of these techniques with classical biological methods is also addressed. Furthermore, the book presents statistical and chemometric methods for evaluation of the resultant data. The broad spectrum of topics includes a vast variety of organisms, samples and diseases, ranging from in vivo metabolomics in humans and animals to in vitro analysis of tissue samples, cultured cells and biofluids.

NMR in Living Systems

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Publisher : Springer Science & Business Media
ISBN 13 : 9400945809
Total Pages : 406 pages
Book Rating : 4.4/5 (9 download)

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Book Synopsis NMR in Living Systems by : T. Axenrod

Download or read book NMR in Living Systems written by T. Axenrod and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 406 pages. Available in PDF, EPUB and Kindle. Book excerpt: In the four decades since its discovery nuclear magnetic resonance (NMR) has become an indispensable tool for obtaining chemical information often. inaccessible by other methods. With the development of instruments of increasingly higher magnetic field strengths, the integration of powerful computers and the availability of an expanding array of flexible software new applications and developments have proliferated rapidly. Among the more exciting new advances is the use of NMR spectroscopy to probe biological systems. The last ten years have witnessed tremendous progress in the development of new NMR imaging and spectroscopic techniques for research and diagnostic applications. The ability to investigate metabolic processes and anatomical structure of intact biological systems under conditions that are totally non-destructive and non-invasive clearly provides much of the impetus for the intense activity that has been generated in the fields of medicine, radiology and the allied basic sciences. Significant advances have been made in this brief period: Whole-body proton NMR imaging today provides anatomical definition of normal and abnormal tissue with a contrast and detection sensitivity often superior to those of X-ray computed tomography and other competing imaging methods. Biochemical pathways, using NMR spectroscopy of protons, carbon-13 and phosphorus-31 nuclei in live animals and man can readily be followed by surface-coil methods to detect metabolites in localized regions. Indicative of the importance and widespread acceptance of these techniques is the explosive growth that the NMR literature is experiencing. This augers well for the future.

Carbon-13 NMR Chemical Shifts in Structural and Stereochemical Analysis

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Author :
Publisher : VCH Publishers
ISBN 13 :
Total Pages : 408 pages
Book Rating : 4.3/5 (91 download)

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Book Synopsis Carbon-13 NMR Chemical Shifts in Structural and Stereochemical Analysis by : Kalevi Pihlaja

Download or read book Carbon-13 NMR Chemical Shifts in Structural and Stereochemical Analysis written by Kalevi Pihlaja and published by VCH Publishers. This book was released on 1994 with total page 408 pages. Available in PDF, EPUB and Kindle. Book excerpt: A review of recent research on strategies and applications of the C-13 chemical shift, a method for determining configuration of organic compounds. Introduces C-13 NMR spectroscopy, and describes conditions for collecting the FID, for data handling, and for obtaining a well- resolved C-13 NMR spectrum, as well as various substituent effect correlations, their derivations, and the origin of the effects. Also discusses the use of multidimensional NMR methods. For organic, physical, and natural products chemists. Includes bandw diagrams. Annotation copyright by Book News, Inc., Portland, OR

Biological Magnetic Resonance

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Publisher : Springer Science & Business Media
ISBN 13 : 0306458861
Total Pages : 265 pages
Book Rating : 4.3/5 (64 download)

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Book Synopsis Biological Magnetic Resonance by : Lawrence Berliner

Download or read book Biological Magnetic Resonance written by Lawrence Berliner and published by Springer Science & Business Media. This book was released on 1999-01-31 with total page 265 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume constitutes a compilation of the latest experiments and theories on a rapidly evolving and maturing field in MRI/MRS, which is the use of the stable isotope 13-C. The 13-C is used to probe the chemistry, mechanism, and function in living systems. All the chapters are written by experts in the field who discuss topics such as `Tracer Theory and the Suitability of 13-C NMR', `Applications of 13-C to Studies of Human Brain Metabolism', etc.

Fundamentals of Protein NMR Spectroscopy

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Publisher : Springer Science & Business Media
ISBN 13 : 1402034997
Total Pages : 543 pages
Book Rating : 4.4/5 (2 download)

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Book Synopsis Fundamentals of Protein NMR Spectroscopy by : Gordon S. Rule

Download or read book Fundamentals of Protein NMR Spectroscopy written by Gordon S. Rule and published by Springer Science & Business Media. This book was released on 2005-10-28 with total page 543 pages. Available in PDF, EPUB and Kindle. Book excerpt: NMR spectroscopy has proven to be a powerful technique to study the structure and dynamics of biological macromolecules. Fundamentals of Protein NMR Spectroscopy is a comprehensive textbook that guides the reader from a basic understanding of the phenomenological properties of magnetic resonance to the application and interpretation of modern multi-dimensional NMR experiments on 15N/13C-labeled proteins. Beginning with elementary quantum mechanics, a set of practical rules is presented and used to describe many commonly employed multi-dimensional, multi-nuclear NMR pulse sequences. A modular analysis of NMR pulse sequence building blocks also provides a basis for understanding and developing novel pulse programs. This text not only covers topics from chemical shift assignment to protein structure refinement, as well as the analysis of protein dynamics and chemical kinetics, but also provides a practical guide to many aspects of modern spectrometer hardware, sample preparation, experimental set-up, and data processing. End of chapter exercises are included to emphasize important concepts. Fundamentals of Protein NMR Spectroscopy not only offer students a systematic, in-depth, understanding of modern NMR spectroscopy and its application to biomolecular systems, but will also be a useful reference for the experienced investigator.

Carbon-13 NMR Spectroscopy

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Publisher : Elsevier
ISBN 13 : 0323145507
Total Pages : 575 pages
Book Rating : 4.3/5 (231 download)

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Book Synopsis Carbon-13 NMR Spectroscopy by : J Stothers

Download or read book Carbon-13 NMR Spectroscopy written by J Stothers and published by Elsevier. This book was released on 2012-12-02 with total page 575 pages. Available in PDF, EPUB and Kindle. Book excerpt: Carbon-13 NMR Spectroscopy focuses on the potential of 13C techniques and the practical difficulties associated with the detection of 13C NMR absorption. This monograph includes a descriptive presentation of 13C shielding results that has been adopted with emphasis on the structural and stereochemical aspects. Organized into four parts encompassing 11 chapters, this book starts with an overview of the characteristics of the NMR signals derived from compounds containing 13C nuclei in natural abundance that are inherently much weaker than those exhibited by protons. This monograph then compares the primary characteristics of 13C NMR with the more familiar proton methods. Other chapters consider the 13C spectra of pyridine, pyridazine, pyrimidine, pyrazine, s-triazine, and s-tetrazine. The final chapter deals with the effects of solute–solvent interactions on the shieldings of other nuclei. This monograph is intended for organic chemists, graduate students, and researchers in various branches of chemistry with an interest in 13C NMR methods as another approach to chemical problems.

Sup 13/C Cross-polarization Magic-angle NMR Studies of Biological Systems

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Publisher :
ISBN 13 :
Total Pages : pages
Book Rating : 4.:/5 (16 download)

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Book Synopsis Sup 13/C Cross-polarization Magic-angle NMR Studies of Biological Systems by :

Download or read book Sup 13/C Cross-polarization Magic-angle NMR Studies of Biological Systems written by and published by . This book was released on 1979 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: The following topics are discussed: use of high-resolution NMR spectroscopy for studies on naturally occurring biological molecules; cross-polarization magic-angle techniques; transfer of magnetic polarization from an abundant magnetic nucleus, /sup 1/H, to a less abundant nucleus, /sup 13/C, via the thermal reservoir; high-resolution /sup 13/C spectra for membrane constituents of bacterial cells; high-resolution /sup 13/C spectra for canine tendon, bovine tendon, and mouse tail, lipid mobilities in various tissue states; comparison of mobility of collagen in canine tendon with that of bovine tendon and rat skin; and measurement of /sup 13/C natural abundance spectra for several polycrystalline and amorphous biomolecular materials. (HLW).

High Resolution NMR Spectroscopy

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Publisher : Elsevier Inc. Chapters
ISBN 13 : 0128083042
Total Pages : 42 pages
Book Rating : 4.1/5 (28 download)

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Book Synopsis High Resolution NMR Spectroscopy by : Rubén H. Contreras

Download or read book High Resolution NMR Spectroscopy written by Rubén H. Contreras and published by Elsevier Inc. Chapters. This book was released on 2013-06-08 with total page 42 pages. Available in PDF, EPUB and Kindle. Book excerpt: In this chapter, the qualitative model described in is applied to show systematic rationalizations in terms of chemical interactions that define well-known trends for chemical shifts corresponding to 13C, 15N, 17O, and 19F isotopes. The theoretical fundamentals for this approach are given in . They could be a bit difficult to follow for readers who do not have a good training in physics and mathematics. However, this difficulty was intended to be overcome by resorting in to describing this approach and providing “physically” several mathematical expressions and describing them in terms of familiar concepts employed frequently in different branches of chemistry and structural biology. The authors believe that once readers understand how easy this approach is and how it facilitates building pictorial representations of how several chemical interactions can be detected by means of high-resolution NMR spectroscopy, the initial problems will be overcome very soon.

Biological Magnetic Resonance

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Publisher : Springer Science & Business Media
ISBN 13 : 146156543X
Total Pages : 317 pages
Book Rating : 4.4/5 (615 download)

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Book Synopsis Biological Magnetic Resonance by : Lawrence Berliner

Download or read book Biological Magnetic Resonance written by Lawrence Berliner and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 317 pages. Available in PDF, EPUB and Kindle. Book excerpt: Judging from the articles published in Biochemistry, magnetic resonance techniques (NMR and ESR) are now among the most popular methods in biochemical research. The series Biological Magnetic Resonance, the fifth volume of which we are proudly presenting, is intended to provide authori tative coverage of topics of current interest. Previous volumes have covered a number of aspects in a thorough and pedagogical fashion rarely found in other publications in this field. Continuing to fulfill the mission of the series, this volume presents a chapter by Baxter, Mackenzie, and Scott on the applications of carbon-13 NMR spectroscopy in investigations of methabolic pathways in vivo. Blom berg and Ruterjans give a comprehensive summary of the use of nitrogen-15 NMR in studies of systems of biological interest. Phosphorus-3I NMR investigations of enzyme systems are described by Rao. Tsai and Bruzik outline the principles of and summarize the state-of-the-art advances in the 18 use of oxygen isotopes e 70 and 0) in phosphorus-3I and oxygen-17 NMR studies of biophosphates. Lipid-protein interactions as reflected in ESR and NMR data are discussed by Devaux. We wish to thank the authors for their cooperation in maintaining the and continued high standards of the series.

Topics in Carbon-13 NMR Spectroscopy

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Publisher :
ISBN 13 :
Total Pages : 520 pages
Book Rating : 4.3/5 (91 download)

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Book Synopsis Topics in Carbon-13 NMR Spectroscopy by : George C. Levy

Download or read book Topics in Carbon-13 NMR Spectroscopy written by George C. Levy and published by . This book was released on 1974 with total page 520 pages. Available in PDF, EPUB and Kindle. Book excerpt: