Beginning Molecular Biology Laboratory Manual

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Molecular Biology Techniques

Author : Heather B. Miller
Publisher : Academic Press
ISBN 13 : 0123855454
Total Pages : 227 pages
Book Rating : 4.1/5 (238 download)

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Book Synopsis Molecular Biology Techniques by : Heather B. Miller

Download or read book Molecular Biology Techniques written by Heather B. Miller and published by Academic Press. This book was released on 2011-10-18 with total page 227 pages. Available in PDF, EPUB and Kindle. Book excerpt: This manual is an indispensable tool for introducing advanced undergraduates and beginning graduate students to the techniques of recombinant DNA technology, or gene cloning and expression. The techniques used in basic research and biotechnology laboratories are covered in detail. Students gain hands-on experience from start to finish in subcloning a gene into an expression vector, through purification of the recombinant protein. The third edition has been completely re-written, with new laboratory exercises and all new illustrations and text, designed for a typical 15-week semester, rather than a 4-week intensive course. The "project approach to experiments was maintained: students still follow a cloning project through to completion, culminating in the purification of recombinant protein. It takes advantage of the enhanced green fluorescent protein - students can actually visualize positive clones following IPTG induction. - Cover basic concepts and techniques used in molecular biology research labs - Student-tested labs proven successful in a real classroom laboratories - Exercises simulate a cloning project that would be performed in a real research lab - "Project" approach to experiments gives students an overview of the entire process - Prep-list appendix contains necessary recipes and catalog numbers, providing staff with detailed instructions

Human Molecular Biology Laboratory Manual

Author : Stefan Surzycki
Publisher : John Wiley & Sons
ISBN 13 : 0470999241
Total Pages : 246 pages
Book Rating : 4.4/5 (79 download)

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Book Synopsis Human Molecular Biology Laboratory Manual by : Stefan Surzycki

Download or read book Human Molecular Biology Laboratory Manual written by Stefan Surzycki and published by John Wiley & Sons. This book was released on 2008-04-15 with total page 246 pages. Available in PDF, EPUB and Kindle. Book excerpt: Human Molecular Biology Laboratory Manual offers a hands-on, state-of-the-art introduction to modern molecular biology techniques as applied to human genome analysis. In eight unique experiments, simple step-by-step instructions guide students through the basic principles of molecular biology and the latest laboratory techniques. This laboratory manual's distinctive focus on human molecular biology provides students with the opportunity to analyze and study their own genes while gaining real laboratory experience. A Background section highlighting the theoretical principles for each experiment. Safety Precautions. Technical Tips. Expected Results. Simple icons indicating tube orientation in centrifuge. Experiment Flow Charts Spiral bound for easy lab use

Molecular Biology

Author : S. K. Gakhar
Publisher :
ISBN 13 : 9789382332305
Total Pages : 0 pages
Book Rating : 4.3/5 (323 download)

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Book Synopsis Molecular Biology by : S. K. Gakhar

Download or read book Molecular Biology written by S. K. Gakhar and published by . This book was released on 2013-09-30 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: The objective of this text is to train young teachers from colleges and research institutions so that they can advance their research in various fields of biology. It will also help students at BSc and MSc level to learn the techniques involved in molecular biology. The book contains four chapters providing step-by-step protocols. In addition, it has general instructions for safety procedures.

Basic Techniques in Molecular Biology

Author : Stefan Surzycki
Publisher : Springer Science & Business Media
ISBN 13 : 3642569684
Total Pages : 442 pages
Book Rating : 4.6/5 (425 download)

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Book Synopsis Basic Techniques in Molecular Biology by : Stefan Surzycki

Download or read book Basic Techniques in Molecular Biology written by Stefan Surzycki and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 442 pages. Available in PDF, EPUB and Kindle. Book excerpt: This laboratory manual gives a thorough introduction to basic techniques. It is the result of practical experience, with each protocol having been used extensively in undergraduate courses or tested in the authors laboratory. In addition to detailed protocols and practical notes, each technique includes an overview of its general importance, the time and expense involved in its application and a description of the theoretical mechanisms of each step. This enables users to design their own modifications or to adapt the method to different systems. Surzycki has been holding undergraduate courses and workshops for many years, during which time he has extensively modified and refined the techniques described here.

Advanced Methods in Molecular Biology and Biotechnology

Author : Khalid Z. Masoodi
Publisher : Academic Press
ISBN 13 : 012824450X
Total Pages : 220 pages
Book Rating : 4.1/5 (282 download)

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Book Synopsis Advanced Methods in Molecular Biology and Biotechnology by : Khalid Z. Masoodi

Download or read book Advanced Methods in Molecular Biology and Biotechnology written by Khalid Z. Masoodi and published by Academic Press. This book was released on 2020-10-28 with total page 220 pages. Available in PDF, EPUB and Kindle. Book excerpt: Advanced Methods in Molecular Biology and Biotechnology: A Practical Lab Manual is a concise reference on common protocols and techniques for advanced molecular biology and biotechnology experimentation. Each chapter focuses on a different method, providing an overview before delving deeper into the procedure in a step-by-step approach. Techniques covered include genomic DNA extraction using cetyl trimethylammonium bromide (CTAB) and chloroform extraction, chromatographic techniques, ELISA, hybridization, gel electrophoresis, dot blot analysis and methods for studying polymerase chain reactions. Laboratory protocols and standard operating procedures for key equipment are also discussed, providing an instructive overview for lab work. This practical guide focuses on the latest advances and innovations in methods for molecular biology and biotechnology investigation, helping researchers and practitioners enhance and advance their own methodologies and take their work to the next level. - Explores a wide range of advanced methods that can be applied by researchers in molecular biology and biotechnology - Features clear, step-by-step instruction for applying the techniques covered - Offers an introduction to laboratory protocols and recommendations for best practice when conducting experimental work, including standard operating procedures for key equipment

Cell and Molecular Biology Lab Manual

Author : David Thompson
Publisher : Createspace Independent Publishing Platform
ISBN 13 : 9781463530082
Total Pages : 170 pages
Book Rating : 4.5/5 (3 download)

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Book Synopsis Cell and Molecular Biology Lab Manual by : David Thompson

Download or read book Cell and Molecular Biology Lab Manual written by David Thompson and published by Createspace Independent Publishing Platform. This book was released on 2011-06-14 with total page 170 pages. Available in PDF, EPUB and Kindle. Book excerpt: A laboratory manual for an undergraduate-level cell and molecular biology course.

Plant Molecular Biology — A Laboratory Manual

Author : Melody S. Clark
Publisher : Springer Science & Business Media
ISBN 13 : 3642878733
Total Pages : 551 pages
Book Rating : 4.6/5 (428 download)

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Book Synopsis Plant Molecular Biology — A Laboratory Manual by : Melody S. Clark

Download or read book Plant Molecular Biology — A Laboratory Manual written by Melody S. Clark and published by Springer Science & Business Media. This book was released on 2013-11-27 with total page 551 pages. Available in PDF, EPUB and Kindle. Book excerpt: Covering the whole range of molecular biology techniques - genetic engineering as well as cytogenetics of plants -, each chapter begins with an introduction to the basic approach. followed by detailed methods with easy-to-follow protocols and comprehensive troubleshooting. The first part introduces basic molecular methodology such as DNA extraction, blotting, production of libraries and RNA cloning, while the second part describes analytical approaches, in particular RAPD and RFLP. The manual concludes with a variety of gene transfer techniques and both molecular and cytological analysis. As such, this will be of great use to both the first-timer and the experienced scientist.

Synthetic Biology: A Lab Manual

Author : Josefine Liljeruhm
Publisher : World Scientific
ISBN 13 : 9814579564
Total Pages : 205 pages
Book Rating : 4.8/5 (145 download)

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Book Synopsis Synthetic Biology: A Lab Manual by : Josefine Liljeruhm

Download or read book Synthetic Biology: A Lab Manual written by Josefine Liljeruhm and published by World Scientific. This book was released on 2014-04-16 with total page 205 pages. Available in PDF, EPUB and Kindle. Book excerpt: Synthetic Biology: A Lab Manual is the first manual for laboratory work in the new and rapidly expanding field of synthetic biology. Aimed at non-specialists, it details protocols central to synthetic biology in both education and research. In addition, it provides all the information that teachers and students from high schools and tertiary institutions need for a colorful lab course in bacterial synthetic biology using chromoproteins and designer antisense RNAs. As a bonus, practical material is provided for students of the annual international Genetically Engineered Machine (iGEM) competition. The manual is based upon a highly successful course at Sweden's Uppsala University and is coauthored by one of the pioneers of synthetic biology and two bioengineering postgraduate students.An inspiring foreword is written by another pioneer in the field, Harvard's George Church: “Synthetic biology is to early recombinant DNA as a genome is to a gene. Is there anything that SynBio will not impact? There was no doubt that the field of SynBio needed ‘A Lab Manual’ such as the one that you now hold in your hands.”

Calculations for Molecular Biology and Biotechnology

Author : Frank H. Stephenson
Publisher : Academic Press
ISBN 13 : 012375691X
Total Pages : 520 pages
Book Rating : 4.1/5 (237 download)

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Book Synopsis Calculations for Molecular Biology and Biotechnology by : Frank H. Stephenson

Download or read book Calculations for Molecular Biology and Biotechnology written by Frank H. Stephenson and published by Academic Press. This book was released on 2010-07-30 with total page 520 pages. Available in PDF, EPUB and Kindle. Book excerpt: Calculations for Molecular Biology and Biotechnology: A Guide to Mathematics in the Laboratory, Second Edition, provides an introduction to the myriad of laboratory calculations used in molecular biology and biotechnology. The book begins by discussing the use of scientific notation and metric prefixes, which require the use of exponents and an understanding of significant digits. It explains the mathematics involved in making solutions; the characteristics of cell growth; the multiplicity of infection; and the quantification of nucleic acids. It includes chapters that deal with the mathematics involved in the use of radioisotopes in nucleic acid research; the synthesis of oligonucleotides; the polymerase chain reaction (PCR) method; and the development of recombinant DNA technology. Protein quantification and the assessment of protein activity are also discussed, along with the centrifugation method and applications of PCR in forensics and paternity testing. - Topics range from basic scientific notations to complex subjects like nucleic acid chemistry and recombinant DNA technology - Each chapter includes a brief explanation of the concept and covers necessary definitions, theory and rationale for each type of calculation - Recent applications of the procedures and computations in clinical, academic, industrial and basic research laboratories are cited throughout the text New to this Edition: - Updated and increased coverage of real time PCR and the mathematics used to measure gene expression - More sample problems in every chapter for readers to practice concepts

Early Development of Xenopus Laevis

Author : Hazel L. Sive
Publisher : CSHL Press
ISBN 13 : 9780879695040
Total Pages : 370 pages
Book Rating : 4.6/5 (95 download)

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Book Synopsis Early Development of Xenopus Laevis by : Hazel L. Sive

Download or read book Early Development of Xenopus Laevis written by Hazel L. Sive and published by CSHL Press. This book was released on 2000 with total page 370 pages. Available in PDF, EPUB and Kindle. Book excerpt: Amphibian embryos are supremely valuable in studies of early vertebrate development because they are large, handle easily, and can be obtained at many interesting stages. And of all the amphibians available for study, the most valuable is Xenopus laevis,which is easy to keep and ovulates at any time of year in response to simple hormone injections. Xenopusembryos have been studied for years but this is a particularly exciting time for the field. Techniques have become available very recently that permit a previously impossible degree of manipulation of gene expression in intact embryos, as well as the ability to visualize the results of such manipulation. As a result, a sophisticated new understanding of Xenopusdevelopment has emerged, which ensures the species’ continued prominent position among the organisms favored for biological investigation. This manual contains a comprehensive collection of protocols for the study of early development in Xenopusembryos. It is written by several of the field’s most prominent investigators in the light of the experience they gained as instructors in an intensive laboratory course taught at Cold Spring Harbor Laboratory since 1991. As a result it contains pointers, hints, and other technical knowledge not readily available elsewhere. This volume is essential reading for all investigators interested in the developmental and cell biology of Xenopusand vertebrates generally. Many of the techniques described here are illustrated in an accompanying set of videotapeswhich are cross-referenced to the appropriate section of the manual.

CELL AND MOLECULAR BIOLOGY

Author : K. V. CHAITANYA
Publisher : PHI Learning Pvt. Ltd.
ISBN 13 : 8120348001
Total Pages : 156 pages
Book Rating : 4.1/5 (23 download)

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Book Synopsis CELL AND MOLECULAR BIOLOGY by : K. V. CHAITANYA

Download or read book CELL AND MOLECULAR BIOLOGY written by K. V. CHAITANYA and published by PHI Learning Pvt. Ltd.. This book was released on 2013-06-21 with total page 156 pages. Available in PDF, EPUB and Kindle. Book excerpt: This laboratory guide, intended for undergraduate and postgraduate students, includes techniques and their protocols ranging from microscopy to in vitro protein synthesis. Experiments relating to chromosomes study and identifying the phases of cell division are explained. The book lucidly deals with the extraction and characteri-zation of chromatin and techniques for studying its modifications, the gene methodology for identification of mutation and the methodology for isolation of nucleic acids from all types of organisms, such as viruses, fungi, plants and animals. All the protocols have been explained following step-by-step method. Different types of electrophoresis and their techniques, including blotting techniques and the methodology for stripping of probes from membranes for reusing the blot, have also been dealt with. Protocols on modern molecular biology techniques—PCR, restriction enzyme digest, DNA isolation, cloning and DNA sequencing—add weightage to the book. It also gives necessary knowledge of different types of stains, staining techniques, buffers, reagents and media used in the protocols. To help students prepare for answering viva voce questions, the book includes MCQs based on the discussed techniques.

Molecular Neuroscience

Author : Rusty Lansford
Publisher :
ISBN 13 : 9781621820130
Total Pages : 0 pages
Book Rating : 4.8/5 (21 download)

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Book Synopsis Molecular Neuroscience by : Rusty Lansford

Download or read book Molecular Neuroscience written by Rusty Lansford and published by . This book was released on 2014 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: A wide variety of powerful molecular techniques have been applied to biology in recent decades, ranging from recombinant DNA technologies to state-of-the-art imaging methods. But the plethora of techniques available combined with the complexities of neurobiological systems can make it difficult for neuroscientists to select and carry out an experimental procedure to effectively address the question at hand. This laboratory manual serves as a comprehensive practical guide to molecular and cellular methods for neuroscientists. It consists of five major sections: Working with Cells, Working with DNA, Working with RNA, Gene Transfer, and Imaging. Each includes step-by-step protocols and discussions of basic and cutting-edge procedures for working in that area. Fundamental techniques include maintaining a sterile working environment, purifying and culturing neural cells, isolating and manipulating DNA and RNA, and understanding and using a microscope. Advanced topics include single-neuron isolation and analysis, in vivo gene delivery and imaging, optogenetics, RNA interference, transgenic technologies, high-throughput analysis of gene expression (e.g., RNA-Seq), and constructing and imaging fluorescent proteins. The manual includes protocols developed in the Advanced Techniques in Molecular Neuroscience course offered annually at Cold Spring Harbor Laboratory, as well as protocols drawn from its best-selling lab manuals. It is an essential resource for all neuroscientists, from graduate students upward, who seek to use molecular techniques to probe the complexities of the nervous system.

Single-molecule Techniques

Author : Paul R. Selvin
Publisher : CSHL Press
ISBN 13 : 087969775X
Total Pages : 511 pages
Book Rating : 4.8/5 (796 download)

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Book Synopsis Single-molecule Techniques by : Paul R. Selvin

Download or read book Single-molecule Techniques written by Paul R. Selvin and published by CSHL Press. This book was released on 2008 with total page 511 pages. Available in PDF, EPUB and Kindle. Book excerpt: Geared towards research scientists in structural and molecular biology, biochemistry, and biophysics, this manual will be useful to all who are interested in observing, manipulating and elucidating the molecular mechanisms and discrete properties of macromolecules.

RNA

Author : Donald Charles Rio
Publisher :
ISBN 13 : 9780879698904
Total Pages : 0 pages
Book Rating : 4.6/5 (989 download)

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Book Synopsis RNA by : Donald Charles Rio

Download or read book RNA written by Donald Charles Rio and published by . This book was released on 2011 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: So much has been learned about RNA in the past ten years that the ability to purify, analyze, and manipulate RNA molecules is now essential in all kinds of bioscience. Initiating RNA research can be intimidating but the new book RNA: A Laboratory Manualprovides a broad range of up-to-date techniques presented in a functional framework, so that any investigator can confidently handle RNA and carry out meaningful experiments, from the most basic to the highly sophisticated. Originating in three of the field's most prominent laboratories, this manual provides the necessary background and strategies for approaching any RNA investigation, as well as detailed protocols and extensive tips and troubleshooting information. It is required reading for every research laboratory in the life sciences.

Phage Display

Author : Carlos F. Barbas
Publisher : CSHL Press
ISBN 13 : 0879697407
Total Pages : 724 pages
Book Rating : 4.8/5 (796 download)

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Book Synopsis Phage Display by : Carlos F. Barbas

Download or read book Phage Display written by Carlos F. Barbas and published by CSHL Press. This book was released on 2001 with total page 724 pages. Available in PDF, EPUB and Kindle. Book excerpt: Phage-display technology has begun to make critical contributions to the study of molecular recognition. DNA sequences are cloned into phage, which then present on their surface the proteins encoded by the DNA. Individual phage are rescued through interaction of the displayed protein with a ligand, and the specific phage is amplified by infection of bacteria. Phage-display technology is powerful but challenging and the aim of this manual is to provide comprehensive instruction in its theoretical and applied so that any scientist with even modest molecular biology experience can effectively employ it. The manual reflects nearly a decade of experience with students of greatly varying technical expertise andexperience who attended a course on the technology at Cold Spring Harbor Laboratory. Phage-display technology is growing in importance and power. This manual is an unrivalled source of expertise in its execution and application.

A BEGINNER’S GUIDE TO PRACTICAL MOLECULAR ENTOMOLOGY

Author : RAMANA NARAVA
Publisher : American Academic Press
ISBN 13 : 1631815040
Total Pages : 121 pages
Book Rating : 4.6/5 (318 download)

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Book Synopsis A BEGINNER’S GUIDE TO PRACTICAL MOLECULAR ENTOMOLOGY by : RAMANA NARAVA

Download or read book A BEGINNER’S GUIDE TO PRACTICAL MOLECULAR ENTOMOLOGY written by RAMANA NARAVA and published by American Academic Press. This book was released on 2020-12-01 with total page 121 pages. Available in PDF, EPUB and Kindle. Book excerpt: This laboratory manual describes laboratory procedures, equipment uses, preparation of reagents, DNA and RNA isolation, electrophoresis, blotting and hybridisation, molecular markers, PCR, DNA barcodes, sequencing methods, gene expression studies, and RNAi. Each chapter consists of an outline of the principles concerned, a schematic explanation of the procedure with elaborated protocols. The book is main geared for students who are relatively new to practical molecular entomology and therefore the emphasis is on making techniques accessible to those individuals. As the author admits, there is a degree of repetition, what this implies is that the majority of the chapters are basically complete and standard thus, there's no looking around for recipes, however in fact it conjointly pushes up the dimensions. The chapters written in this book gives the background information which can be allowed to modify at their end and design new protocols.

Plant Biotechnology and Molecular Biology : A Laboratory Manual

Author : M.S. Punia
Publisher : Scientific Publishers
ISBN 13 : 9388172361
Total Pages : 263 pages
Book Rating : 4.3/5 (881 download)

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Book Synopsis Plant Biotechnology and Molecular Biology : A Laboratory Manual by : M.S. Punia

Download or read book Plant Biotechnology and Molecular Biology : A Laboratory Manual written by M.S. Punia and published by Scientific Publishers. This book was released on 2018-01-01 with total page 263 pages. Available in PDF, EPUB and Kindle. Book excerpt: The book, “A Laboratory Manual of Plant Biotechnology and Molecular Biology” comprises of workable laboratory protocols for a large number of techniques related to plant biotechnology, genetic engineering and molecular biology. This includes plant cell and tissue culture, callus and suspension culture, anther culture, ovule culture, embryo culture, Cryopreservation, Isolation of Plant protoplasts, Protoplast culture and regeneration, production of somatic hybrids through protoplast fusion, gene transformation using Agrobacterium as vector, direct gene transfer using biolistic gun, Isolation of plant and organells DNA, construction and screening of genomic DNA libraries, Molecular markers like RFLP, RAPD, SCARS and CAPS, DNA sequencing, RNA isolation and northern blotting, Isolation of proteins and western blotting etc. The manual is prepared with the objective to cater the needs of post- graduate students as well as for scientists working in the disciplines of Plant Breeding, Genetics, Botany, Plant physiology, Biochemistry, Plant Biotechnology, Molecular Biology etc. It gives an update on some well established methods and presents reliable protocols.