Atomic Force Microscopy In Molecular And Cell Biology

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Atomic Force Microscopy

Author : Pier Carlo Braga
Publisher : Springer Science & Business Media
ISBN 13 : 1592596479
Total Pages : 388 pages
Book Rating : 4.5/5 (925 download)

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Book Synopsis Atomic Force Microscopy by : Pier Carlo Braga

Download or read book Atomic Force Microscopy written by Pier Carlo Braga and published by Springer Science & Business Media. This book was released on 2008-02-02 with total page 388 pages. Available in PDF, EPUB and Kindle. Book excerpt: The natural, biological, medical, and related sciences would not be what they are today without the microscope. After the introduction of the optical microscope, a second breakthrough in morphostructural surface analysis occurred in the 1940s with the development of the scanning electron microscope (SEM), which, instead of light (i. e. , photons) and glass lenses, uses electrons and electromagnetic lenses (magnetic coils). Optical and scanning (or transmission) electron microscopes are called “far-field microscopes” because of the long distance between the sample and the point at which the image is obtained in comparison with the wavelengths of the photons or electrons involved. In this case, the image is a diffraction pattern and its resolution is wavelength limited. In 1986, a completely new type of microscopy was proposed, which, without the use of lenses, photons, or electrons, directly explores the sample surface by means of mechanical scanning, thus opening up unexpected possibilities for the morphostructural and mechanical analysis of biological specimens. These new scanning probe microscopes are based on the concept of near-field microscopy, which overcomes the problem of the limited diffraction-related resolution inherent in conventional microscopes. Located in the immediate vicinity of the sample itself (usually within a few nanometers), the probe records the intensity, rather than the interference signal, thus significantly improving resolution. Since the most we- known microscopes of this type operate using atomic forces, they are frequently referred to as atomic force microscopes (AFMs).

Atomic Force Microscopy

Author : Nuno C. Santos
Publisher : Humana Press
ISBN 13 : 9781493988938
Total Pages : 372 pages
Book Rating : 4.9/5 (889 download)

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Book Synopsis Atomic Force Microscopy by : Nuno C. Santos

Download or read book Atomic Force Microscopy written by Nuno C. Santos and published by Humana Press. This book was released on 2018-10-30 with total page 372 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book aims to provide examples of applications of atomic force microscopy (AFM) using biological samples, showing different methods for AFM sample preparation, data acquisition and processing, and avoiding technical problems. Divided into two sections, chapters guide readers through image artifacts, process and quantitatively analyze AFM images, lipid bilayers, image DNA-protein complexes, AFM cell topography, single-molecule force spectroscopy, single-molecule dynamic force spectroscopy, fluorescence methodologies, molecular recognition force spectroscopy, biomechanical characterization, AFM-based biosensor setup, and detail how to implement such an in vitro system, which can monitor cardiac electrophysiology, intracellular calcium dynamics, and single cell mechanics. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Atomic Force Microscopy: Methods and Protocols is useful for researchers at different stages, from newcomers to experienced users, interested in new AFM applications.

Atomic Force Microscopy in Molecular and Cell Biology

Author : Jiye Cai
Publisher : Springer
ISBN 13 : 9811315108
Total Pages : 244 pages
Book Rating : 4.8/5 (113 download)

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Book Synopsis Atomic Force Microscopy in Molecular and Cell Biology by : Jiye Cai

Download or read book Atomic Force Microscopy in Molecular and Cell Biology written by Jiye Cai and published by Springer. This book was released on 2018-11-03 with total page 244 pages. Available in PDF, EPUB and Kindle. Book excerpt: The book addresses new achievements in AFM instruments – e.g. higher speed and higher resolution – and how AFM is being combined with other new methods like NSOM, STED, STORM, PALM, and Raman. This book explores the latest advances in atomic force microscopy and related techniques in molecular and cell biology. Atomic force microscopy (AFM) can be used to detect the superstructures of the cell membrane, cell morphology, cell skeletons and their mechanical properties. Opening up new fields of in-situ dynamic study for living cells, enzymatic reactions, fibril growth and biomedical research, these combined techniques will yield valuable new insights into molecule and cell biology. This book offers a valuable resource for students and researchers in the fields of biochemistry, cell research and chemistry etc.

Atomic Force Microscopy in Cell Biology

Author :
Publisher : Academic Press
ISBN 13 : 0080549446
Total Pages : 430 pages
Book Rating : 4.0/5 (85 download)

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Book Synopsis Atomic Force Microscopy in Cell Biology by :

Download or read book Atomic Force Microscopy in Cell Biology written by and published by Academic Press. This book was released on 2002-05-30 with total page 430 pages. Available in PDF, EPUB and Kindle. Book excerpt: This is the first book to cover the history, structure, and application of atomic force microscopy in cell biology. Presented in the clear, well-illustrated style of the Methods in Cell Biology series, it introduces the AFM to its readers and enables them to tap the power and scope of this technology to further their own research. A practical laboratory guide for use of the atomic force and photonic force microscopes, it provides updated technology and methods in force spectroscopy. It is also a comprehensive and easy-to-follow practical laboratory guide for the use of the AFM and PFM in biological research.

Intermolecular and Surface Forces

Author : Jacob N. Israelachvili
Publisher : Academic Press
ISBN 13 : 0123919339
Total Pages : 708 pages
Book Rating : 4.1/5 (239 download)

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Book Synopsis Intermolecular and Surface Forces by : Jacob N. Israelachvili

Download or read book Intermolecular and Surface Forces written by Jacob N. Israelachvili and published by Academic Press. This book was released on 2011-07-22 with total page 708 pages. Available in PDF, EPUB and Kindle. Book excerpt: Intermolecular and Surface Forces describes the role of various intermolecular and interparticle forces in determining the properties of simple systems such as gases, liquids and solids, with a special focus on more complex colloidal, polymeric and biological systems. The book provides a thorough foundation in theories and concepts of intermolecular forces, allowing researchers and students to recognize which forces are important in any particular system, as well as how to control these forces. This third edition is expanded into three sections and contains five new chapters over the previous edition. - Starts from the basics and builds up to more complex systems - Covers all aspects of intermolecular and interparticle forces both at the fundamental and applied levels - Multidisciplinary approach: bringing together and unifying phenomena from different fields - This new edition has an expanded Part III and new chapters on non-equilibrium (dynamic) interactions, and tribology (friction forces)

Open-Space Microfluidics

Author : Emmanuel Delamarche
Publisher : John Wiley & Sons
ISBN 13 : 3527696792
Total Pages : 450 pages
Book Rating : 4.5/5 (276 download)

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Book Synopsis Open-Space Microfluidics by : Emmanuel Delamarche

Download or read book Open-Space Microfluidics written by Emmanuel Delamarche and published by John Wiley & Sons. This book was released on 2018-01-18 with total page 450 pages. Available in PDF, EPUB and Kindle. Book excerpt: Summarizing the latest trends and the current state of this research field, this up-to-date book discusses in detail techniques to perform localized alterations on surfaces with great flexibility, including microfluidic probes, multifunctional nanopipettes and various surface patterning techniques, such as dip pen nanolithography. These techniques are also put in perspective in terms of applications and how they can be transformative of numerous (bio)chemical processes involving surfaces. The editors are from IBM Zurich, the pioneers and pacesetters in the field at the forefront of research in this new and rapidly expanding area.

Cell Imaging Techniques

Author : Douglas J. Taatjes
Publisher : Springer Science & Business Media
ISBN 13 : 1592599931
Total Pages : 505 pages
Book Rating : 4.5/5 (925 download)

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Book Synopsis Cell Imaging Techniques by : Douglas J. Taatjes

Download or read book Cell Imaging Techniques written by Douglas J. Taatjes and published by Springer Science & Business Media. This book was released on 2008-02-04 with total page 505 pages. Available in PDF, EPUB and Kindle. Book excerpt: A diverse collection of state-of-the-art methods for the microscopic imaging of cells and molecules. The authors cover a wide spectrum of complimentary techniques, including such methods as fluorescence microscopy, electron microscopy, atomic force microscopy, and laser scanning cytometry. Additional readily reproducible protocols on confocal scanning laser microscopy, quantitative computer-assisted image analysis, laser-capture microdissection, microarray image scanning, near-field scanning optical microscopy, and reflection contrast microscopy round out this eclectic collection of cutting-edge imaging techniques now available. The authors also discuss preparative methods for particles and cells by transmission electron microscopy.

Life at the Nanoscale

Author : Yves Dufrene
Publisher : CRC Press
ISBN 13 : 981426797X
Total Pages : 460 pages
Book Rating : 4.8/5 (142 download)

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Book Synopsis Life at the Nanoscale by : Yves Dufrene

Download or read book Life at the Nanoscale written by Yves Dufrene and published by CRC Press. This book was released on 2019-03-28 with total page 460 pages. Available in PDF, EPUB and Kindle. Book excerpt: Proceeding from basic fundamentals to applications, this volume provides a comprehensive overview of the use of AFM and related scanning probe microscopies for cell surface analysis. It covers all cell types, from viruses and protoplasts to bacteria and animal cells. It also discusses a range of advanced AFM modalities, including high-resolution imaging, nanoindentation measurements, recognition imaging, and single-molecule and single-cell force spectroscopy. The book covers methodologies for preparing and analyzing cells and membranes of all kinds and highlights recent examples to illustrate the power of AFM techniques in life sciences and nanomedicine.

Molecular Biology of the Cell

Author :
Publisher :
ISBN 13 : 9780815332183
Total Pages : 0 pages
Book Rating : 4.3/5 (321 download)

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Book Synopsis Molecular Biology of the Cell by :

Download or read book Molecular Biology of the Cell written by and published by . This book was released on 2002 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Bioimaging

Author : Douglas E. Chandler
Publisher : Jones & Bartlett Publishers
ISBN 13 : 0763738743
Total Pages : 468 pages
Book Rating : 4.7/5 (637 download)

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Book Synopsis Bioimaging by : Douglas E. Chandler

Download or read book Bioimaging written by Douglas E. Chandler and published by Jones & Bartlett Publishers. This book was released on 2009 with total page 468 pages. Available in PDF, EPUB and Kindle. Book excerpt: The Development Of Microscopy Revolutionized The World Of Cell And Molecular Biology As We Once Knew It And Will Continue To Play An Important Role In Future Discoveries. Bioimaging: Current Concepts In Light And Electron Microscopy Is The Optimal Text For Any Undergraduate Or Graduate Bioimaging Course, And Will Serve As An Important Reference Tool For The Research Scientist. This Unique Text Covers, In Great Depth, Both Light And Electron Microscopy, As Well As Other Structure And Imaging Techniques Like X-Ray Crystallography And Atomic Force Microscopy. Written In A User-Friendly Style And Covering A Broad Range Of Topics, Bioimaging Describes The State-Of-The-Art Technologies That Have Powered The Field To The Forefront Of Cellular And Molecular Biological Research.

Fundamentals and Application of Atomic Force Microscopy for Food Research

Author : Jian Zhong
Publisher : Academic Press
ISBN 13 : 0128241322
Total Pages : 385 pages
Book Rating : 4.1/5 (282 download)

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Book Synopsis Fundamentals and Application of Atomic Force Microscopy for Food Research by : Jian Zhong

Download or read book Fundamentals and Application of Atomic Force Microscopy for Food Research written by Jian Zhong and published by Academic Press. This book was released on 2022-09-29 with total page 385 pages. Available in PDF, EPUB and Kindle. Book excerpt: Fundamentals and Application of Atomic Force Microscopy for Food Research explains how to get reliable AFM data and current application progress of AFM in different food substances. Sections focus on an Introduction to AFM for food research and Applications of AFM for different types of food substances. Edited by 3 experts in the field of nanotechnology and food science, this book reduces the difficulty of AFM application and shortens the learning time for new hands. Until now, no such book has systematically described the application of Atomic Force Microscopy (AFM) for food research. Many scientists in the field of food science and engineering need to evaluate their developed foods and food contact surfaces at nanoscale. However, there is a steep learning curve for new hands, hence the need for this comprehensive resource. - Describes the application of AFM for food research - Covers applications of AFM for different types of food substances - Addresses future uses and perspectives of AFM for the development of food nanotechnology

Physics of Bio-Molecules and Cells

Author : Henrik Flyvbjerg
Publisher : Springer Science & Business Media
ISBN 13 : 3540457011
Total Pages : 591 pages
Book Rating : 4.5/5 (44 download)

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Book Synopsis Physics of Bio-Molecules and Cells by : Henrik Flyvbjerg

Download or read book Physics of Bio-Molecules and Cells written by Henrik Flyvbjerg and published by Springer Science & Business Media. This book was released on 2003-07-01 with total page 591 pages. Available in PDF, EPUB and Kindle. Book excerpt: Aimed at those working to enter this rapidly developing field, this volume on biological physics is written in a pedagogical style by leading scientists giving explanations that take their starting point where any physicist can follow and end at the frontier of research in biological physics. These lectures describe the state-of-the-art physics of biomolecules and cells. In biological systems ranging from single biomolecules to entire cells and larger biological systems, it focuses on aspects that require concepts and methods from physics for their analysis and understanding, such as the mechanics of motor proteins; how the genetic code is physically read and managed; the machinery of protein--DNA interactions; force spectroscopy of biomolecules' velopes, cytoskeletons, and cytoplasms; polymerization forces; listeria propulsion; cell motility; lab-on-a-chip nanotechnology for single-molecule analysis of biomolecules; bioinformatics; and coding and computational strategies of the brain.

Live Cell Imaging

Author : Robert D. Goldman
Publisher : CSHL Press
ISBN 13 : 9780879696832
Total Pages : 658 pages
Book Rating : 4.6/5 (968 download)

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Book Synopsis Live Cell Imaging by : Robert D. Goldman

Download or read book Live Cell Imaging written by Robert D. Goldman and published by CSHL Press. This book was released on 2005 with total page 658 pages. Available in PDF, EPUB and Kindle. Book excerpt: Recent advances in imaging technology reveal, in real time and great detail, critical changes in living cells and organisms. This manual is a compendium of emerging techniques, organized into two parts: specific methods such as fluorescent labeling, and delivery and detection of labeled molecules in cells; and experimental approaches ranging from the detection of single molecules to the study of dynamic processes in organelles, organs, and whole animals. Although presented primarily as a laboratory manual, the book includes introductory and background material and could be used as a textbook in advanced courses. It also includes a DVD containing movies of living cells in action, created by investigators using the imaging techniques discussed in the book. The editors, David Spector and Robert Goldman, whose previous book was Cells: A Laboratory Manual,are highly respected investigators who have taught microscopy courses at Cold Spring Harbor Laboratory, the Marine Biology Laboratory at Woods Hole, and Northwestern University.

Atomic Force Microscopy in Liquid

Author : Arturo M. Baró
Publisher : John Wiley & Sons
ISBN 13 : 3527327584
Total Pages : 385 pages
Book Rating : 4.5/5 (273 download)

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Book Synopsis Atomic Force Microscopy in Liquid by : Arturo M. Baró

Download or read book Atomic Force Microscopy in Liquid written by Arturo M. Baró and published by John Wiley & Sons. This book was released on 2012-05-14 with total page 385 pages. Available in PDF, EPUB and Kindle. Book excerpt: About 40 % of current atomic force microscopy (AFM) research is performed in liquids, making liquid-based AFM a rapidly growing and important tool for the study of biological materials. This book focuses on the underlying principles and experimental aspects of AFM under liquid, with an easy-to-follow organization intended for new AFM scientists. The book also serves as an up-to-date review of new AFM techniques developed especially for biological samples. Aimed at physicists, materials scientists, biologists, analytical chemists, and medicinal chemists. An ideal reference book for libraries. From the contents: Part I: General Atomic Force Microscopy * AFM: Basic Concepts * Carbon Nanotube Tips in Atomic Force Microscopy with * Applications to Imaging in Liquid * Force Spectroscopy * Atomic Force Microscopy in Liquid * Fundamentals of AFM Cantilever Dynamics in Liquid * Environments * Single-Molecule Force Spectroscopy * High-Speed AFM for Observing Dynamic Processes in Liquid * Integration of AFM with Optical Microscopy Techniques Part II: Biological Applications * DNA and Protein-DNA Complexes * Single-Molecule Force Microscopy of Cellular Sensors * AFM-Based Single-Cell Force Spectroscopy * Nano-Surgical Manipulation of Living Cells with the AFM

Correlative Light and Electron Microscopy IV

Author :
Publisher : Academic Press
ISBN 13 : 0128220597
Total Pages : 452 pages
Book Rating : 4.1/5 (282 download)

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Book Synopsis Correlative Light and Electron Microscopy IV by :

Download or read book Correlative Light and Electron Microscopy IV written by and published by Academic Press. This book was released on 2021-03-09 with total page 452 pages. Available in PDF, EPUB and Kindle. Book excerpt: Correlative Light and Electron Microscopy IV, Volume 162, a new volume in the Methods in Cell Biology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. Besides the detailed description of protocols for CLEM technologies including time-resolution, Super resolution LM and Volume EM, new chapters cover Workflow (dis)-advantages/spiderweb, Serial section LM + EM, Platinum clusters as CLEM probes, Correlative Light Electron Microscopy with a transition metal complex as a single probe, SEM-TEM-SIMS, HPF-CLEM, A new workflow for high-throughput screening of mitotic mammalian cells for electron microscopy using classic histological dyes, and more. - Contains contributions from experts in the field - Covers topics using nano-SIMS and EDX for CLEM - Presents recent advances and currently applied correlative approaches - Gives detailed protocols, allowing for the application of workflows in one's own laboratory setting - Covers CLEM approaches in the context of specific applications - Aims to stimulate the use of new combinations of imaging modalities

Molecular Manipulation with Atomic Force Microscopy

Author : Anne-Sophie Duwez
Publisher : CRC Press
ISBN 13 : 1439809666
Total Pages : 291 pages
Book Rating : 4.4/5 (398 download)

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Book Synopsis Molecular Manipulation with Atomic Force Microscopy by : Anne-Sophie Duwez

Download or read book Molecular Manipulation with Atomic Force Microscopy written by Anne-Sophie Duwez and published by CRC Press. This book was released on 2011-12-07 with total page 291 pages. Available in PDF, EPUB and Kindle. Book excerpt: With the invention of scanning probe techniques in the early 1980s, scientists can now play with single atoms, single molecules, and even single bonds. Force, dynamics, and function can now be probed at the single-molecule level. Molecular Manipulation with Atomic Force Microscopy (AFM) presents a series of topics that discuss concepts and methodologies used to manipulate and study single (bio)molecules with AFM. The first part is dedicated to the pulling of single molecules with force spectroscopy to investigate molecular interactions, mechanics, and mechanochemical processes, and the second part to the manipulation, repositioning, and targeted delivery of single molecules on substrates. Single molecule manipulation is an exciting area of research which made important breakthroughs in nanoscience and which could find potential applications in a diverse range of disciplines, including chemistry, biology, physics, material and polymer science, and engineering. New and experienced AFM researchers looking for applications beyond imaging will find a wealth of information in this informative volume.

Atomic Force Microscopy for Nanoscale Biophysics

Author : Mi Li
Publisher : Elsevier
ISBN 13 : 0323958338
Total Pages : 338 pages
Book Rating : 4.3/5 (239 download)

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Book Synopsis Atomic Force Microscopy for Nanoscale Biophysics by : Mi Li

Download or read book Atomic Force Microscopy for Nanoscale Biophysics written by Mi Li and published by Elsevier. This book was released on 2023-02-15 with total page 338 pages. Available in PDF, EPUB and Kindle. Book excerpt: Atomic Force Microscopy for Nanoscale Biophysics: From Single Molecules to Living Cells summarizes the applications of atomic force microscopy for the investigation of biomolecules and cells. The book discusses the methodology of AFM-based biomedical detection, diverse biological systems, and the combination of AFM with other complementary techniques. These state-of-the-art chapters empower researchers to address biological issues through the application of atomic force microscopy. Atomic force microscopy (AFM) is a unique, multifunctional tool for investigating the structures and properties of living biological systems under aqueous conditions with unprecedented spatiotemporal resolution. - Summarizes the recent progress of atomic force microscopy in biomedical applications - Presents the methods and skills of applying atomic force microscopy - Aids researchers in investigating the nanoscale biophysics of diverse biological systems