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Assay Of Protein And Polypeptide Hormones
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Book Synopsis Assay of Protein and Polypeptide Hormones by : H. Van Cauwenberge
Download or read book Assay of Protein and Polypeptide Hormones written by H. Van Cauwenberge and published by Elsevier. This book was released on 2014-05-17 with total page 255 pages. Available in PDF, EPUB and Kindle. Book excerpt: International Series of Monographs in Pure and Applied Biology, Modern Trends in Physiological Sciences, Volume 33: Assay of Protein and Polypeptide Hormones focuses on the assays of protein and polypeptide hormones. The selection first discusses the general principles of protein and polypeptide hormone assays, including conditions and basic factors for bioassays and status of biological assays. The book looks at the assays of hypophyseal hormones, such as growth hormones, adrenocorticotropin (ACTH), thyrotropin, gonadotropins, prolactin, vasopressin, and oxytocin. Concerns include radioimmunoassay, standards of thyrotropin, immunoassay of hypophyseal gonadotropins, and bioassay of prolactin. The book evaluates the assays of pancreatic hormones, such as insulin and glucagon. Chemical structure and properties of insulin and radioimmunoassay of glucagon are explained. The text also focuses on the assays of erythropoietin, renin, human chorionic gonadotropin, and human placental lactogen (HPL). Concerns include augmentation of red cell volume; biological application of renin assays; and similarities and differences between hypophyseal somatotropin and HPL. The selection is a valuable source of data for readers interested in the assays of protein and polypeptide hormones.
Book Synopsis The Cytochemical Bioassay of Polypeptide Hormones by : J. Chayen
Download or read book The Cytochemical Bioassay of Polypeptide Hormones written by J. Chayen and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 336 pages. Available in PDF, EPUB and Kindle. Book excerpt: The cytochemical bioassay system was described in a short abstract in 1971, and more fully, in the cytochemical bioassay of corticotrophin, in 1972. Since then, cytochemical bioassays have been described for several polypeptide hormones, and these assays are already widely used. It is expedient that the subject should be reviewed, as it is in this monograph, by one writer who has had the good fortune to have taken part in the growth of cytochemistry from its early origins to its present position as the basis of possibly the most sensitive bioassay system currently available. However, it should be noted that major contributions have been made by many, both to the development of the subject and to the establishment of the bioassays. The object of this preface is to try to give some perspective to the growth of this subject and to record that the cytochemical bioassay system has been fostered by many outstanding scientists in an atmosphere of remarkable goodwill. To begin with, there could have been no cytochemical bioassays until cytochem istry had been converted from its rather unsure origins into a precise and quantitative form of cellular biochemistry. This was done with skill and enthusiastic dedication by my colleagues, Dr. Lucille Bitensky, Dr. F. P. Altman, Dr. R. G. L. W. Poulter and Mr. A. A. Silcox, first at the Royal College of Butcher, Dr.
Book Synopsis Structure-activity Relationships of Protein and Polypeptide Hormones by : M. Margoulies
Download or read book Structure-activity Relationships of Protein and Polypeptide Hormones written by M. Margoulies and published by . This book was released on 1972 with total page 604 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Methods of Protein Microcharacterization by : John E. Shively
Download or read book Methods of Protein Microcharacterization written by John E. Shively and published by Springer Science & Business Media. This book was released on 2008-02-22 with total page 458 pages. Available in PDF, EPUB and Kindle. Book excerpt: Milestones in the techniques and methodology of polypeptide structure determination include the determination of the sequence of insulin by Sanger in 1951 (I) and the introduction of the repeti tive degradation of proteins with phenylisothiocyanate by Edman in 1959 (2). The automation of Edman chemistry (3) played a major role in the determination of polypeptide structures. Important modifications of Edman chemistry include the solid-phase approach by Laursen in 1971 (4) and the use of modified Edman reagents such as 4-N, N-dimethylaminoazobenzene-4'-isothiocy- ate (DABITC) for manual sequencing by Chang et al. (5) in 1976. A second major breakthrough in the analysis of polypeptides was automated amino acid analysis described by Spackman et al. in 1958 (6). However, during the period from 1975 to 1980, it became increasingly clear that the amount of material required for struc tural analysis was more than could be easily isolated for the vast majority of proteins. The field was criticized for its lack of sensitive techniques for the analysis of growth factors, immune modulators, membrane receptors, and peptide hormones. In addition, very little had been done to modernize and improve the original instruments introduced in the mid-1960s. The first indications of improved instrumentation for Edman chemistry came from Wittmann-Liebold's laboratory (7), followed by the introduction of a "micro" sequencer by Hunkapiller and Hood in 1978 (8). The movement toward improved instrumentation culminated in the "gas"--Phase sequencer of Hewick et al. (9) in 1981
Author :Brigitte Wittmann-Liebold Publisher :Springer Science & Business Media ISBN 13 :3642715346 Total Pages :427 pages Book Rating :4.6/5 (427 download)
Book Synopsis Advanced Methods in Protein Microsequence Analysis by : Brigitte Wittmann-Liebold
Download or read book Advanced Methods in Protein Microsequence Analysis written by Brigitte Wittmann-Liebold and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 427 pages. Available in PDF, EPUB and Kindle. Book excerpt: Much of the recent spectacular progress in the biological sciences can be at tributed ot the ability to isolate, analyze, and structurally characterize proteins and peptides which are present in cells and cellular organelles in only very small amounts. Recent advances in protein chemistry and in particular the application of new micromethods have led to fruitful advances in the understanding of basic cellular processes. Areas where protein-chemical studies have resulted in interest ing discoveries include the peptide hormones and their release factors, growth factors and oncogenes, bioenergetics, proton pumps and ion pumps and chan nels, topogenesis and protein secretion, molecular virology and immunology, membrane protein analysis, and receptor research. In fact, the key methods are now on hand to unravel many of the major outstanding problems of molecular biology and in particular questions of fundamental interest which relate to devel opmental biology and specificity in cell-cell interaction. In this volume we have assembled descriptions of procedures which have re cently been shown to be efficaceous for the isolation, purification, and chemical characterization of proteins and peptides that are only available in minute amounts. Emphasis is placed on well-established micromethods which have been tested and found useful in many laboratories by experienced investigators. The chapters are written by specialists, and describe a range of sensitive techniques which can be used by researchers working in laboratories with only modest resources and equipment.
Book Synopsis Polypeptide Hormones by : Ruth Porter
Download or read book Polypeptide Hormones written by Ruth Porter and published by John Wiley & Sons. This book was released on 2009-09-16 with total page 192 pages. Available in PDF, EPUB and Kindle. Book excerpt: The Novartis Foundation Series is a popular collection of the proceedings from Novartis Foundation Symposia, in which groups of leading scientists from a range of topics across biology, chemistry and medicine assembled to present papers and discuss results. The Novartis Foundation, originally known as the Ciba Foundation, is well known to scientists and clinicians around the world.
Book Synopsis Pharmacology of Hormonal Polypeptides and Proteins by : Nathan Back
Download or read book Pharmacology of Hormonal Polypeptides and Proteins written by Nathan Back and published by Springer. This book was released on 2013-12-11 with total page 671 pages. Available in PDF, EPUB and Kindle. Book excerpt: It can be concluded (under the specific experimental procedures em ployed) that:- 1) HCG labelled with 1 - 2 atoms of radioactive iodine did not differ sig nificantly from the unlabelled hormone; 2) The ovary alone exhibited a capacity to affix specifically HCG; 3) The amount of radioactive material in the ovary was directly proport ional to the quantity of labelled HCG injected; 4) When the HCG present in the circulation is bound to an antiserum to HCG, the antigen-antibody complex is not concentrated by the ovary; 5) Circulating labelled HCG decreased to 50% within 30 minutes following a single intravenous injection; 6) There are four different phases of ovarian uptake of HCG, namely: the first phase, when there is only an inflow from the circulation and stor age mainly in the follicular envelopes; the second phase, when there is a greater inflow than outflow; the third phase, when the inflow is equal to the outflow; and the fourth period, when the outflow is bigger than the in flow. REFERENCES 1. Lunenfeld, B. and Eshkol, A. Vitamins and Hormones (1967) 25:165 2. Eshkol, A. In: Recent Research on Gonadotropbio Hormones, eds. E. T. Bell andJ. A. Loraine, Edinburgh, Livingstone (1967), p. 202. 3. Eshkol, A. and Lunenfeld, B. Proc. Tel-Hashomer Hosp. (1967) 6:4. ACKNOWLEOOMEN'IS This work was supported in part by a grant from the Population Council, N. Y. , U. S. A. andbyGrantNo.
Book Synopsis Chemistry, Biology, and Medicine of Neurohypophyseal Hormones and Their Analogs by : Sidney Udenfriend
Download or read book Chemistry, Biology, and Medicine of Neurohypophyseal Hormones and Their Analogs written by Sidney Udenfriend and published by Academic Press. This book was released on 2013-10-22 with total page 383 pages. Available in PDF, EPUB and Kindle. Book excerpt: Chemistry, Biology, and Medicine of Neurohypophyseal Hormones and Their Analogs, Volume 8 covers the advances in methods to study peptide hormone action at the molecular level. The book discusses the anatomy, physiology, and clinical disorders of the neurohypophysis; the biosynthesis of neurohypophyseal peptides; and the structure-activity relationships of neurohypophyseal peptides, with an emphasis on the role of conformational features. The text also describes conformational studies of neurohypophyseal hormones by physical chemical techniques; the central nervous system effects of neurohypophyseal peptides; and the effects of neurohypophyseal hormones on the kidney. The effects of neurohypophyseal hormones on the cardiovascular systems are also considered. Chemists, neurobiologists, and people involved in the study of neurohypophyseal hormones will find the book invaluable.
Book Synopsis Molecular Biology of The Cell by : Bruce Alberts
Download or read book Molecular Biology of The Cell written by Bruce Alberts and published by . This book was released on 2002 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Structure-activity Relationships of Protein and Polypeptide Hormones by :
Download or read book Structure-activity Relationships of Protein and Polypeptide Hormones written by and published by . This book was released on 1971 with total page 604 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Protein and Polypeptide Hormones by : M. Margoulies
Download or read book Protein and Polypeptide Hormones written by M. Margoulies and published by . This book was released on 1968 with total page 986 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Hormone Chemistry: Protein, polypeptide & peptide hormones by : Wilfrid R. Butt
Download or read book Hormone Chemistry: Protein, polypeptide & peptide hormones written by Wilfrid R. Butt and published by . This book was released on 1975 with total page 296 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Peptide Hormone Secretion by : K. Siddle
Download or read book Peptide Hormone Secretion written by K. Siddle and published by Oxford University Press, USA. This book was released on 1990 with total page 376 pages. Available in PDF, EPUB and Kindle. Book excerpt: Polypeptide hormones play a central role in the regulation of biological processes from the minute-to-minute control of vital functions to the programming of birth, development, reproduction, and ageing. The first of a two-volume work on the methods of study used in this field, Peptide Hormone Secretion focuses on the isolation of polypeptide hormones and their identification and structural analysis, the development of sensitive bioassay and immunoassay techniques, and tissue localization of peptide hormones and their encoding MRNAs, as well as techniques for characterization of peptide hormone receptors. Ideal for graduate students and researchers, the book includes coverage of the general principles and strategies that the methods are based on, so the techniques will have a wide application to biologically active peptides involved in neurotransmission, growth regulations, differentiation, and vasoregulation.
Book Synopsis Hormonal Proteins and Peptides by : Choh Hao Li
Download or read book Hormonal Proteins and Peptides written by Choh Hao Li and published by . This book was released on 1973 with total page 472 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Plant Peptide Hormones and Growth Factors by : Andreas Schaller
Download or read book Plant Peptide Hormones and Growth Factors written by Andreas Schaller and published by Springer Nature. This book was released on 2023-11-29 with total page 312 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book describes state-of-the-art approaches for identification and functional characterization of plant peptide hormones and growth factors. Chapters guide readers through protocols for computational identification of novel signaling peptides and phytocytokines, analysis of post-translational maturation events, isolation of mature peptides from plant tissues, peptide bioassays, in vivo and in vitro analysis of peptide activity, peptide receptor identification and interaction analysis, and quantitative assessment of peptide ligand binding to cognate receptors. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Plant Peptide Hormones and Growth Factors aims to be a useful and practical guide to new researchers and experts looking to expand their technical know-how.
Book Synopsis Principles of Competitive Protein-binding Assays by : William D. Odell
Download or read book Principles of Competitive Protein-binding Assays written by William D. Odell and published by Lippincott Williams & Wilkins. This book was released on 1971 with total page 462 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Immunological Properties of Protein Hormones by : Filippo Polvani
Download or read book Immunological Properties of Protein Hormones written by Filippo Polvani and published by Elsevier. This book was released on 2012-12-02 with total page 274 pages. Available in PDF, EPUB and Kindle. Book excerpt: Immunological Properties of Protein Hormones covers the proceedings of the 1964 International Endocrinological Symposia on Immunological Properties of Protein Hormones, held in Rome, Italy. This symposium is organized under the auspices of Accademia Nazionale dei Lincei and of Consiglio Nazionale delle Rieerche Rome. This book is organized into four sections encompassing 27 chapters that tackle the immunological properties of gonadotropins, growth hormones, and insulin. The first section presents first an overview of immunological studies of protein hormones, followed by a discussion on the application of immunofluorescence to localization of pituitary hormones. This section also looks into the antigenic property of ACTH antibodies. The remaining sections cover the immunoassay methods of analysis, occurrence, distribution, clinical and physiological aspects, functions, and antigenic properties of the specific protein hormones. This book is of great benefit to immunoendocrinologists.
