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Aptamer Based Biosensors For Protein Biomarkers
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Book Synopsis Aptamer-based Biosensors for Protein Biomarkers by : Alan Ka Ho Cheng
Download or read book Aptamer-based Biosensors for Protein Biomarkers written by Alan Ka Ho Cheng and published by . This book was released on 2009 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: The technique of "systematic evolution of ligands by exponential enrichment" has transformed the premise of biorecognition to encompass nucleic acids in addition to antibodies.These synthetic counterparts - aptamers - are DNA or RNA strands that have been selected through iterative rounds of in vitro selection to bind with affinity to analytes (from small molecules to proteins) of choice. In this thesis, the voltammetric aptamer-based detection of lysozyme was first explored based on charge density changes on aptamer-modified electrodes upon analyte binding. Different optical and electrochemical methods were then tested for the detection of ubiquitous glycanhydrolaselysozyme was first explored based on charge density changes on aptamer-modified electrodes upon analyte binding. Different detection methodologies (optical and electrochemical methods) were then tested for the detection of MUC1(mucin 1),a glycoprotein overexpressed in elevated concentrations in the serum of patients with epithelial adenocarcinomas; this augments the importance for MUC1 detection as an alternative for ealry cancer diagnostics. Optical biosensors developed for the MUC1 detection was through a DNA hybridization scheme with quantum-dot based flouorescence readout. Electrochemical methods were based on either direct attachment of a redox center, methylene blue(MB), to the aptamer strands,or adding free MB to the electrolyte solution(intercalative binding). In both cases, quantitative detection of MUC1 was possible, although direct labeling showed a better sensitivity. To further improve the sensitivity, an intricate biochemical assay was explored featuring the fusion of anti-thrombin and anti-MUC1 aptamers, in the hope of generating a pico-Molar assay for MUC1 via the MUC1- dependent allosteric upregulation of the proteolytic activity of human a-thrombin.
Book Synopsis Biosensors Based on Aptamers and Enzymes by : Man Bock Gu
Download or read book Biosensors Based on Aptamers and Enzymes written by Man Bock Gu and published by Springer. This book was released on 2014-07-08 with total page 338 pages. Available in PDF, EPUB and Kindle. Book excerpt: Volumes are organized topically and provide a comprehensive discussion of developments in the respective field over the past 3-5 years. The series also discusses new discoveries and applications. Special volumes are dedicated to selected topics which focus on new biotechnological products and new processes for their synthesis and purification. In general, special volumes are edited by well-known guest editors. The series editor and publisher will however always be pleased to receive suggestions and supplementary information. Manuscripts are accepted in English.
Book Synopsis Study of Aptamer Selection Methodologies for Developing Piezoelectric Quartz Crystal Biosensors to Detect Albumin in Urine, Malaria and Sars Protein Biomarkers in Serum by : Dharmatov Rahula Barlongo Albano
Download or read book Study of Aptamer Selection Methodologies for Developing Piezoelectric Quartz Crystal Biosensors to Detect Albumin in Urine, Malaria and Sars Protein Biomarkers in Serum written by Dharmatov Rahula Barlongo Albano and published by . This book was released on 2017-01-26 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: This dissertation, "Study of Aptamer Selection Methodologies for Developing Piezoelectric Quartz Crystal Biosensors to Detect Albumin in Urine, Malaria and SARS Protein Biomarkers in Serum" by Dharmatov Rahula Barlongo, Albano, was obtained from The University of Hong Kong (Pokfulam, Hong Kong) and is being sold pursuant to Creative Commons: Attribution 3.0 Hong Kong License. The content of this dissertation has not been altered in any way. We have altered the formatting in order to facilitate the ease of printing and reading of the dissertation. All rights not granted by the above license are retained by the author. Abstract: Due to the high affinity and selectivity of aptamer selected from DNA library towards target proteins, the use of specific protein biomarkers to assist early diagnosis, and the recent rapid development of piezoelectric quartz crystal (PQC) biosensor technology, the research reported is focused on developing PQC biosensor using aptamers selected from a DNA library as selective coating for the detection of albumin in urine, two protein biomarkers for malaria in sera, and the determination of trace amount of SCV helicase protein from SAR in sera. Three new aptamer-PQC biosensors are developed with research conducted on aptamer selection, optimization of coating methods for aptamers on PQC, and coupling paramagnetic nanoparticle technology with PQC biosensor in flow injection analysis (FIA) to enrich analyte and eliminate sample matrix interference. A new approach for aptamer selection is successfully attempted in the development of the first aptamer-PQC biosensor with an aim to offer an early detection of microalbuminuria enabled by an increase in detection sensitivity with aptamer selected by its actual binding with albumin-PQC biosensor. The aptamer-PQC sensor developed has shown to give a working range between 0.1 to 10 ug/mL, a detection limit (S/N = 3, n=3) of 0.048 μg/ml, repeatability of RSD = 6.8% (n=3), a response time of 1 minute and a throughput of 60 samples/hour. The developed piezoelectric aptamer sensor is shown to have sufficient sensitivity to detect microgram quantities of albumin in urine. Aptamer-PQC biosensors are developed for the detection of two protein biomarkers, PfLDH for general diagnosis and PfHRP-2 for a specific diagnosis of Plasmodium falciparum malaria parasitemia, in sera. The results show satisfactory working range from 10-100 ng/mL for both PfLDH and PfHRP-2 protein biomarker, low detection limit of 1.8 and 4.7 ng/mL and satisfactory repeatability (%RSD, n = 3) of 7.4% and 9.2% for PfHRP-2 and PfLDH respectively. It is first report for aptamer based PQC biosensor to detect malarial PfHRP-2 and PfLDH at ppb range to meet the requirement for their diagnosis. To meet the highly demanding challenge for detecting protein biomarkers at pg/mL level in high-protein sera sample, the coupling of paramagnetic nanoparticle technology with aptamer-PQC biosensor was attempted for developing aptamer-PQC biosensor for selective detection of SCV helicase protein produced from SARS CoV replication. The coupling of aptamer coated paramagnetic nanoparticles for sample pretreatment to aptamer-PQC biosensor has shown to detect helicase protein in one-minute assay with a detection limit of 350 pg/mL. The aptamer-coated crystal exhibits a frequency shift linearly proportional to the concentration of SARS helicase from 1 to 1000 ng/mL with a correlation coefficient of 0.9975 and a repeatability of 6.8% (%RSD, n=3). After the enrichment procedure, recoveries of 102% and 119% were achieved using samples spiked with SARS helicase at concentrations of 10 ng/mL and 1.0 ng/mL respectively. It is the first report to detect for SCV helicase protein using PQC biosensor at pg/mL level after magnetic bead enrichment. DOI: 10.5353/th_b5185935 Subjects: Biochemical markers Albumins Piezoelectric polymer biosensors
Book Synopsis Aptasensors for Point-of-Care Diagnostics by : Arpana Parihar
Download or read book Aptasensors for Point-of-Care Diagnostics written by Arpana Parihar and published by CRC Press. This book was released on 2023-12-18 with total page 375 pages. Available in PDF, EPUB and Kindle. Book excerpt: The design and fabrication of aptamer-based biosensors for point-of-care testing of disease diagnostic and prognostic is an upthrust and interdisciplinary area of research. This book covers the design and development of novel cost-effective aptamer-based biosensors for disease diagnostic and prognostic including an understanding of health care management in terms of timely updates of disease situations in a particular geographical area. It also discusses the scaling up and market trend of aptamer-based devices for extension of research from lab to market, and end users. Features: Explains the design and fabrication of aptasensors-based diagnostic devices. Includes novel approaches and comprehensive technology overview for diagnosis management towards early-stage detection of various biomarkers associated with several health conditions. Demonstrates possible benefits of combined diagnostics and therapeutic possibilities using aptamer-based point-of-care technologies devices. Discusses emerging implications and recent advances of biosensing platforms for designing and developing aptamer-based point-of-care testing (POCT) devices. Explores aptamer-based approach to develop advanced platforms for medical applications and open arena for state-of-the-art future research. This book is aimed at researchers and graduate students in biomedical engineering, diagnostics, nanobiotechnology, and materials science.
Book Synopsis Computational Design and Experimental Validation of RNA-Based Biosensors for the Detection of Biomarker Proteins by : Lipika Gadila
Download or read book Computational Design and Experimental Validation of RNA-Based Biosensors for the Detection of Biomarker Proteins written by Lipika Gadila and published by . This book was released on 2018 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: There is a need to detect medically relevant proteins such as biomarkers of human disease. Aptamers are nucleic acid-based recognition elements that have the ability to selectively bind to a large range of targets with high affinity, including proteins. Specifically, our objective is to engineer RNA-based riboswitches, which use aptamers to bind to a target ligand and thereby alter gene expression. Riboswitches can be potentially engineered as diagnostic sensors for protein targets. Compared to enzyme-linked immunosorbent assays (ELISAs) or surface plasmon resonance (SPR), riboswitch sensors have not been extensively tested for their ability to detect protein biomarkers of human disease. This thesis aims to validate a method of computationally designing riboswitches that can bind to and detect a specific protein of interest. Creating a riboswitch for a specific aptamer can be a slow process without computational modeling. As a result, the riboswitches in these experiments have been designed with the Riboswitch Calculator. Riboswitches, were designed to detect three proteins of interest: MS2 coat protein, C-reactive protein (CRP), and Interleukin-32. Aptamers for these protein targets were identified and used to design the riboswitch sequences. The riboswitches and expression cassettes were tested in a coupled transcription-translation in vitro gene expression system known as TX-TL. Two designed riboswitches successfully detected the MS2 coat protein with statistically significant (p
Book Synopsis NanoBioMedicine by : Shailendra K. Saxena
Download or read book NanoBioMedicine written by Shailendra K. Saxena and published by Springer Nature. This book was released on 2020-02-03 with total page 510 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book provides a comprehensive overview of the recent trends in various Nanotechnology-based therapeutics and challenges associated with its development. Nanobiotechnology is an interdisciplinary research that has wide applications in the various fields of biomedical research. The book discusses the various facets of the application of Nanotechnology in drug delivery, clinical diagnostics, Nanomedicine and treatment of infectious and chronic diseases. The book also highlights the recent advancements on important devices and applications that are based on Nanotechnology in medicine and brief the regulatory and ethical issues related to nanomedical devices. It also reviews the toxicological profile of various nanomaterials and emphasizes the need for safe nanomaterials for clinical use. Finally, the book discusses the recent developments of potential commercial applications of Nanotechnology.
Download or read book Biosensors written by Inamuddin and published by Materials Research Forum LLC. This book was released on 2019-04-20 with total page 324 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book presents recent developments in the field of biosensors and their applications in healthcare. Topics include aptasensors for the detection of environmental contaminants, disease-causing pathogens, molecularly imprinted polymers for the detection of genetic materials, infectious diseases, in vivo monitoring of key molecules, functional nanoparticles targeted to specific tumor cells for detection as well as imaging.
Book Synopsis Advanced Biosensors for Health Care Applications by : Dr. Inamuddin
Download or read book Advanced Biosensors for Health Care Applications written by Dr. Inamuddin and published by Elsevier. This book was released on 2019-06-15 with total page 432 pages. Available in PDF, EPUB and Kindle. Book excerpt: Advanced Biosensors for Health Care Applications highlights the different types of prognostic and diagnostic biomarkers associated with cancer, diabetes, Alzheimer's disease, brain and retinal diseases, cardiovascular diseases, bacterial infections, as well as various types of electrochemical biosensor techniques used for early detection of the potential biomarkers of these diseases. Many advanced nanomaterials have attracted intense interests with their unique optical and electrical properties, high stability, and good biocompatibility. Based on these properties, advanced nanoparticles have been used as biomolecular carriers, signal producers, and signal amplifiers in biosensor design. Recent studies reported that there are several diagnostic methods available, but the major issue is the sensitivity and selectivity of these approaches. This book outlines the need of novel strategies for developing new systems to retrieve health information of patients in real time. It explores the potential of nano-multidisciplinary science in the design and development of smart sensing technology using micro-nanoelectrodes, novel sensing materials, integration with MEMS, miniaturized transduction systems, novel sensing strategy, that is, FET, CMOS, System-on-a-Chip (SoC), Diagnostic-on-a-Chip (DoC), and Lab-on-a-Chip (LOC), for diagnostics and personalized health-care monitoring. It is a useful handbook for specialists in biotechnology and biochemical engineering. Describes advanced nanomaterials for biosensor applications Relates the properties of available nanomaterials to specific biomarkers applications Includes diagnosis and electrochemical studies based on biosensors Explores the potential of nano-multidisciplinary science to design and develop smart sensing technologies Describes novel strategies for developing a new class of assay systems to retrieve the desired health information
Book Synopsis Electrochemical Biosensors by : Serge Cosnier
Download or read book Electrochemical Biosensors written by Serge Cosnier and published by CRC Press. This book was released on 2015-01-26 with total page 405 pages. Available in PDF, EPUB and Kindle. Book excerpt: Since four decades, rapid detection and monitoring in clinical and food diagnostics and in environmental and biodefense have paved the way for the elaboration of electrochemical biosensors. Thanks to their adaptability, ease of use in relatively complex samples, and their portability, electrochemical biosensors now are one of the mainstays of analy
Book Synopsis The Selective and Sensitive Detection of Protein Biomarkers Using Aptamer-based Nanosensors by : Benjamin James Edwards
Download or read book The Selective and Sensitive Detection of Protein Biomarkers Using Aptamer-based Nanosensors written by Benjamin James Edwards and published by . This book was released on 2020 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Biosensors Based on Sandwich Assays by : Fan Xia
Download or read book Biosensors Based on Sandwich Assays written by Fan Xia and published by Springer. This book was released on 2018-01-09 with total page 220 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book shows the various sandwich assays that are constructed from recognition molecules, such as antibodies, oligonucleotide sequences and aptamers, developed as a result of nano- and biotechnology advances. It consists of ten chapters presenting interesting examples of these assays, organized according to the type of analytic methods (colorimetric, fluorescence, electrochemical, etc.) and detected objects (protein, nucleic acid, small-molecule, ion, etc.). It also includes a chapter discussing the introduction of sandwich assays as biosensors for the detection of a range of targets. It is an interesting and useful resource for a wide readership in various fields of chemical science and nanotechnology.
Book Synopsis Aptamers for Medical Applications by : Yiyang Dong
Download or read book Aptamers for Medical Applications written by Yiyang Dong and published by Springer Nature. This book was released on 2021-03-25 with total page 470 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book outlines comprehensively the main medical uses of aptamers, from diagnosis to therapeutics in fourteen chapters. Pioneering topics covered include aptamer pharmaceuticals, aptamers for malign tumors, aptamers for personalized therapeutics and aptamers for point-of-care testing. The book offers an essential guide for medical scientists interested in developing aptamer-based schemes for better theranostics. It is therefore of interest for not only academic researchers, but also practitioners and medical researchers in various fields of medical science, medical research and bio-analytical chemistry.
Book Synopsis Aptamers for Analytical Applications by : Yiyang Dong
Download or read book Aptamers for Analytical Applications written by Yiyang Dong and published by John Wiley & Sons. This book was released on 2019-02-11 with total page 426 pages. Available in PDF, EPUB and Kindle. Book excerpt: An essential guide that puts the focus on method developments and applications in aptamers In recent years, aptamer-based systems have been developed for a wide-range of analytical and medical applications. Aptamers for Analytical Applications offers an introduction to the topic, outlines the common protocols for aptamer synthesis, as well as providing information on the different optimization strategies that can obtain higher affinities to target molecules. The contributors?noted experts on the topic?provide an in-depth review of the characterization of aptamer-target molecule interaction and immobilization strategies and discuss the developments of methods for all the relevant applications. The book outlines different schemes to efficiently immobilize aptamers on substrates as well as summarizing the characterization methods for aptamer-ligand complexes. In addition, aptamer-based colorimetric, enzyme-linked, fluorescent, electrochemical, lateral flow and non-labeling analytical methods are presented. The book also reflects state-of-the-art and emerging applications of aptamer-based methods. This important resource: -Provides a guide to aptamers which provide highly specific and sensitive molecular recognition, with affinities in the range of antibodies and are much cheaper to produce -Offers a discussion of the analytical method developments and improvements with established systems and beyond -Offers a comprehensive guide to all the relevant application areas -Presents an authoritative book from contributors who are noted experts in the field Written for analytical chemists, biochemists, analytical researchers, Aptamers for Analytical Applications is a comprehensive book that adopts a methodological point of view to the important aspects of aptamer generation and modification with a strong emphasis on method developments for relevant applications.
Book Synopsis Development of an Electrochemical Surface-enhanced Raman Spectroscopy DNA Aptamer Biosensor for Rapid Detection of Protein Biomarkers of Disease by : Scott Gerald Harroun
Download or read book Development of an Electrochemical Surface-enhanced Raman Spectroscopy DNA Aptamer Biosensor for Rapid Detection of Protein Biomarkers of Disease written by Scott Gerald Harroun and published by . This book was released on 2014 with total page 372 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Electrochemical Biosensors by : Ali A. Ensafi
Download or read book Electrochemical Biosensors written by Ali A. Ensafi and published by Elsevier. This book was released on 2019-07-25 with total page 388 pages. Available in PDF, EPUB and Kindle. Book excerpt: Electrochemical Biosensors summarizes fundamentals and trends in electrochemical biosensing. It introduces readers to the principles of transducing biological information to measurable electrical signals to identify and quantify organic and inorganic substances in samples. The complexity of devices related to biological matrices makes this challenging, but this measurement and analysis are critically valuable in biotechnology and medicine. Electrochemical biosensors combine the sensitivity of electroanalytical methods with the inherent bioselectivity of the biological component. Some of these sensor devices have reached the commercial stage and are routinely used in clinical, environmental, industrial and agricultural applications. Describes several electrochemical methods used as detection techniques with biosensors Discusses different modifiers, including nanomaterials, for preparing suitable pathways for immobilizing biomaterials at the sensor Explains various types of signal monitoring, along with several recognition systems, including antibodies/antigens, DNA-based biosensors, aptamers (protein-based), and more
Book Synopsis Electrochemical Aptamer-Based Biosensors for Disease Biomarkers by : Masoud Negahdary
Download or read book Electrochemical Aptamer-Based Biosensors for Disease Biomarkers written by Masoud Negahdary and published by Elsevier. This book was released on 2025-02-01 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Electrochemical Aptamer-Based Biosensors for Disease Biomarkers comprehensively presents the principles of designing aptamer-based biosensors for disease detection using biomarkers. This book considers the latest research conducted in recent years and discusses the application of aptasensors design against different diseases and explores innovative types of electrochemical diagnostic techniques used based on the use of various advanced elements and assemblies such as nanomaterials and signal transducers. Electrochemical Aptamer-Based Biosensors for Disease Biomarkers is an effective practical guide for researchers to use as reference for the development of their own laboratory research.
Book Synopsis Aptamers in Biotechnology by : Katharina Urmann
Download or read book Aptamers in Biotechnology written by Katharina Urmann and published by Springer Nature. This book was released on 2020-07-31 with total page 217 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book reviews the development, characterization and applications of aptamers in different areas of biotechnology ranging from therapeutics to diagnostics and protein purification. Hailed as chemical antibodies, these single-stranded nucleic acid receptors were predicted to supersede antibodies in traditional assays, such as ELISA, within a short time. While this has yet to happen, readers will find in this book a deep insight into the progress of aptamer technology and a critical discussion about the limitations that need to be overcome in order to find wider acceptance and use outside of the still relatively small aptamer-community. This book covers all aspects of aptamer generation and application for the aptamer-experienced reader and curious novice alike, with the addition of an industry perspective on the future of aptamer-use in biotechnology.
