Analytical Affinity Chromatography

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Analytical Affinity Chromatography

Author : Irwin M. Chaiken
Publisher : CRC Press
ISBN 13 : 1351078127
Total Pages : 204 pages
Book Rating : 4.3/5 (51 download)

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Book Synopsis Analytical Affinity Chromatography by : Irwin M. Chaiken

Download or read book Analytical Affinity Chromatography written by Irwin M. Chaiken and published by CRC Press. This book was released on 2018-01-10 with total page 204 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume presents discussions of theoretical and experimental considerations that have led to the analytical affinity chromatography field, as well as current efforts to use this methodology to characterize the interaction mechanisms of biological macromolecules and to establish conditions for employing bioaffinity chromatographic systems as preparative tools. The chapters include a comprehensive discussion of interactive chromatography theory (DeLisi and Hethcote), a review of experimental data obtained for biological macromolecules and the relevant theoretical considerations of affinity chromatography which led to them (Swaisgood and Chaiken), an evaluation of rate processes in affinity chromatography and the potential to determine biologically meaningful chemical rate constants (Walters), and the use of quantitative and molecular considerations to design affinity chromatographic systems (Stellwagen and Liu).

Affinity Chromatography

Author : Pascal Bailon
Publisher : Springer Science & Business Media
ISBN 13 : 1603272615
Total Pages : 233 pages
Book Rating : 4.6/5 (32 download)

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Book Synopsis Affinity Chromatography by : Pascal Bailon

Download or read book Affinity Chromatography written by Pascal Bailon and published by Springer Science & Business Media. This book was released on 2008-02-04 with total page 233 pages. Available in PDF, EPUB and Kindle. Book excerpt: Affinity chromatography, with its exquisite specificity, is based upon molecular recognition. It is a powerful tool for the purification of biomolecules. In recent years, numerous new applications and modified techniques have been derived from gro- specific interactions and biological recognition principles. An up-to-date review of the past, current, and future applications of affinity chromatography has been presented in the introductory chapter by Meir Wilchek and Irwin Chaiken. Though many of these new applications and techniques are well documented in the literature, it is often difficult to find methods that are written with the intent of helping new practitioners of affinity chromatography. This volume on Affinity Chromatography: Methods and Protocols is intended for the novice, as well as for - perts in the field. The protocols are written by experts who have developed and/or successfully employed these methods in their laboratories. Each chapter describes a specific technique, and since the book is intended to help the beginner, each technique is described simply and clearly, making sure that all relevant steps are included, assuming no previous knowledge. Each chapter contains an introduction describing the principles involved, followed by a Materials and Methods section, which lays the groundwork for the reader to conduct experiments step-by-step, in an orderly fashion. The following Notes section, which describes many of the problems that can occur, makes suggestions for overcoming them, and provides alternate procedures. These are precisely the sort of important, practical details that never seem to appear in the published literature.

Approaches to the Purification, Analysis and Characterization of Antibody-Based Therapeutics

Author : Allan Matte
Publisher : Elsevier
ISBN 13 : 0081030193
Total Pages : 222 pages
Book Rating : 4.0/5 (81 download)

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Book Synopsis Approaches to the Purification, Analysis and Characterization of Antibody-Based Therapeutics by : Allan Matte

Download or read book Approaches to the Purification, Analysis and Characterization of Antibody-Based Therapeutics written by Allan Matte and published by Elsevier. This book was released on 2020-09-07 with total page 222 pages. Available in PDF, EPUB and Kindle. Book excerpt: Approaches to the Purification, Analysis and Characterization of Antibody-Based Therapeutics provides the interested and informed reader with an overview of current approaches, strategies and considerations relating to the purification, analytics and characterization of therapeutic antibodies and related molecules. While there are obviously other books published in and around this subject area, they seem to be either older (c.a. year 2000 publication date) or are more limited in scope. The book will include an extensive bibliography of the published literature in the respective areas covered. It is not, however, intended to be a how-to methods book. Covers the vital new area of R&D on therapeutic antibodies Written by leading scientists and researchers Up-to-date coverage and includes a detailed bibliography

Analytical Affinity Chromatography

Author :
Publisher :
ISBN 13 :
Total Pages : pages
Book Rating : 4.:/5 (16 download)

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Book Synopsis Analytical Affinity Chromatography by :

Download or read book Analytical Affinity Chromatography written by and published by . This book was released on 2017 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:

Affinity Chromatography

Author : P. Mohr
Publisher : CRC Press
ISBN 13 : 9780824774684
Total Pages : 320 pages
Book Rating : 4.7/5 (746 download)

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Book Synopsis Affinity Chromatography by : P. Mohr

Download or read book Affinity Chromatography written by P. Mohr and published by CRC Press. This book was released on 1985-11-22 with total page 320 pages. Available in PDF, EPUB and Kindle. Book excerpt: Affinity Chromatography combines theoretical aspects and practical applications-providing a solid understanding of affinity principles on a molecular level. Beginning with the historical background of affinity chromatography, this single-source volume discusses matrix supports and the insertion of spacers ... the chemical and physicochemical features of the adsorption and elution step ... immunoassay technology and separation of viruses and cells ... variants of affinity chromatography other than biospecific ... related techniques including affinity electrophoresis ... and much more. Written by leading experts in the field, Affinity Chromatography contains convenient features such as a concise outline format-summarizing useful information; numerous illustrations-clarifying fundamental and methodical factors; easy-to-read tables-for a quick understanding of vital material; current references-facilitating continuing study. Providing stimulation for the development of new affinity methods, this important volume is mandatory for analytical chemists, chromatographers, biochemists, biologists, microbiologists, pharmacists, and students in advanced undergraduate- and graduate-level analytical and preparative chemistry courses. Book jacket.

Liquid Chromatography

Author : D.S. Hage
Publisher : Elsevier Inc. Chapters
ISBN 13 : 0128071060
Total Pages : 40 pages
Book Rating : 4.1/5 (28 download)

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Book Synopsis Liquid Chromatography by : D.S. Hage

Download or read book Liquid Chromatography written by D.S. Hage and published by Elsevier Inc. Chapters. This book was released on 2013-01-08 with total page 40 pages. Available in PDF, EPUB and Kindle. Book excerpt: Affinity chromatography is a type of liquid chromatography that uses a biologically related agent as a stationary phase to purify or analyze specific sample components. This chapter examines the basic components and principles of affinity chromatography and various applications of this method. The general types of ligands, immobilization schemes, support materials, and elution conditions that can be employed in affinity chromatography are first examined. Various methods in this field are then considered, including bioaffinity chromatography, immunoaffinity chromatography, dye-ligand and biomimetic affinity chromatography, immobilized metal-ion affinity chromatography, and analytical affinity chromatography. The types of ligands utilized in each of these methods are described, along with common applications of these techniques. Several related developments are also discussed, such as the use of affinity chromatography with mass spectrometry, miniaturized systems, molecularly imprinted polymers, and aptamers.

Development of Analytical Uses of High Performance Affinity Chromatography

Author : Mandi L. Conrad
Publisher :
ISBN 13 :
Total Pages : 318 pages
Book Rating : 4.:/5 (259 download)

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Book Synopsis Development of Analytical Uses of High Performance Affinity Chromatography by : Mandi L. Conrad

Download or read book Development of Analytical Uses of High Performance Affinity Chromatography written by Mandi L. Conrad and published by . This book was released on 2008 with total page 318 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Affinity Chromatography and Biological Recognition

Author : Irwin Chaiken
Publisher : Elsevier
ISBN 13 : 0323146627
Total Pages : 542 pages
Book Rating : 4.3/5 (231 download)

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Book Synopsis Affinity Chromatography and Biological Recognition by : Irwin Chaiken

Download or read book Affinity Chromatography and Biological Recognition written by Irwin Chaiken and published by Elsevier. This book was released on 2012-12-02 with total page 542 pages. Available in PDF, EPUB and Kindle. Book excerpt: Affinity Chromatography and Biological Recognition contains manuscripts presented at the Fifth International Symposium on Affinity Chromatography and Biological Recognition convened in June 12-17, 1983, at St. John's College in Annapolis, Maryland. Organized into six parts encompassing 82 chapters, the book begins by examining the growing synergism between affinity methods and the understanding and study of basic principles of biological recognition. The book then focuses on the trends and progress in the design and application of affinity methods for isolation, therapeutics, diagnostics, and biotechnology. Significant chapters are devoted to the contributions of affinity methodology in such areas as cell membrane receptors, quantitative properties of macromolecular interactions, microscale analytical and preparative applications of high performance affinity chromatography, antibodies as in vivo and in vitro diagnostic and therapeutic agents, and drug targeting. This volume will be a stimulus for broad and creative application of affinity concepts and methods in many fields of biomedical research and biotechnology.

Handbook of Affinity Chromatography

Author : David S. Hage
Publisher : CRC Press
ISBN 13 : 0824751981
Total Pages : 878 pages
Book Rating : 4.8/5 (247 download)

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Book Synopsis Handbook of Affinity Chromatography by : David S. Hage

Download or read book Handbook of Affinity Chromatography written by David S. Hage and published by CRC Press. This book was released on 2005-07-19 with total page 878 pages. Available in PDF, EPUB and Kindle. Book excerpt: This essential handbook guides investigators in the theory, applications, and practical use of affinity chromatography in a variety of fields including biotechnology, biochemistry, molecular biology, analytical chemistry, proteomics, pharmaceutical science, environmental analysis, and clinical chemistry. The Handbook of Affinity Chromatograph

Chromatography

Author : E. Heftmann
Publisher : Elsevier
ISBN 13 : 0080472249
Total Pages : 585 pages
Book Rating : 4.0/5 (84 download)

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Book Synopsis Chromatography by : E. Heftmann

Download or read book Chromatography written by E. Heftmann and published by Elsevier. This book was released on 2004-04-16 with total page 585 pages. Available in PDF, EPUB and Kindle. Book excerpt: Chromatography has emerged as the most important and versatile analytical method. The book is not only an updated version of Heftmann's classical text, but it covers areas of future importance, such as microfluidics and computer resources. Under his experienced guidance, authorities in each field have contributed their practical experience to an integrated treatment of modern micro analysis. In Part A the theoretical basis of individual separation methods is explained and the technical aspects are illustrated. It includes the theory of gas and liquid chromatography as well as specific chromatographic techniques, such as size-exclusion, planar, ion, and affinity chromatography as well as various electrokinetic separation techniques. Microfluidics are covered for the first time and useful sources of analytical instruments are listed and evaluated. 1. Each chapter written by an authority2. Thorough treatment of the theoretical basis of separation methods3. Practical guide for performing analyses

Analytical and Preparative Separation Methods of Biomacromolecules

Author : Hassan Y. Aboul-Enein
Publisher : CRC Press
ISBN 13 : 1000146251
Total Pages : 482 pages
Book Rating : 4.0/5 (1 download)

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Book Synopsis Analytical and Preparative Separation Methods of Biomacromolecules by : Hassan Y. Aboul-Enein

Download or read book Analytical and Preparative Separation Methods of Biomacromolecules written by Hassan Y. Aboul-Enein and published by CRC Press. This book was released on 2020-10-15 with total page 482 pages. Available in PDF, EPUB and Kindle. Book excerpt: Reports up-to-date research developments on purifying and isolation large organic molecules. The text provides information on high-performance liquid chromatography and capillary electrophoresis (CE) as tools for analyzing biomacromolecules and developing new biochemical and medicinal compounds. It applies biochemical separation technology to the study of macromolecules such as proteins, polysaccharides, nucleic acids and more.

Handbook of Affinity Chromatography, Second Edition

Author : David S. Hage
Publisher : CRC Press
ISBN 13 : 9780824740573
Total Pages : 968 pages
Book Rating : 4.7/5 (45 download)

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Book Synopsis Handbook of Affinity Chromatography, Second Edition by : David S. Hage

Download or read book Handbook of Affinity Chromatography, Second Edition written by David S. Hage and published by CRC Press. This book was released on 2005-07-19 with total page 968 pages. Available in PDF, EPUB and Kindle. Book excerpt: This essential handbook guides investigators in the theory, applications, and practical use of affinity chromatography in a variety of fields including biotechnology, biochemistry, molecular biology, analytical chemistry, proteomics, pharmaceutical science, environmental analysis, and clinical chemistry. The Handbook of Affinity Chromatography reflects upon important factors to consider in the development of affinity methods, such as the choice of support material, immobilization methods, and application or elution conditions. It reviews common affinity methods and explores the latest preparative, analytical, and biophysical applications, including the use of affinity chromatography with other separation techniques and analytical systems. This basis seamlessly supports the discussion of recent developments in techniques including the use of affinity ligands in capillary electrophoresis, mass spectrometry, microanalytical systems, and optical biosensors. New chapters feature expanded discussions on molecularly imprinted polymers and biomimetic ligands, chromatographic immunoassays, affinity-based immunoassays, affinity-based chiral stationary phases, and affinity ligands in multidimensional systems. Engaging the collaboration of 48 scientists and students from 23 laboratories and organizations to present the latest information on affinity methods, the Handbook of Affinity Chromatography illustrates a wide range of applications and theory for scientists, students, and laboratory workers throughout the fields of chemistry and biology.

Current Developments in Biotechnology and Bioengineering

Author : Ashok Pandey
Publisher : Elsevier
ISBN 13 : 0444636730
Total Pages : 888 pages
Book Rating : 4.4/5 (446 download)

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Book Synopsis Current Developments in Biotechnology and Bioengineering by : Ashok Pandey

Download or read book Current Developments in Biotechnology and Bioengineering written by Ashok Pandey and published by Elsevier. This book was released on 2016-09-17 with total page 888 pages. Available in PDF, EPUB and Kindle. Book excerpt: Current Developments in Biotechnology and Bioengineering: Production, Isolation and Purification of Industrial Products provides extensive coverage of new developments, state-of-the-art technologies, and potential future trends, focusing on industrial biotechnology and bioengineering practices for the production of industrial products, such as enzymes, organic acids, biopolymers, and biosurfactants, and the processes for isolating and purifying them from a production medium. During the last few years, the tools of molecular biology and genetic and metabolic engineering have rendered tremendous improvements in the production of industrial products by fermentation. Structured by industrial product classifications, this book provides an overview of the current practice, status, and future potential for the production of these agents, along with reviews of the industrial scenario relating to their production. Provides information on industrial bioprocesses for the production of microbial products by fermentation Includes separation and purification processes of fermentation products Presents economic and feasibility assessments of the various processes and their scaling up Links biotechnology and bioengineering for industrial process development

Affinity Chromatography

Author : Sameh Magdeldin
Publisher : BoD – Books on Demand
ISBN 13 : 9535103253
Total Pages : 360 pages
Book Rating : 4.5/5 (351 download)

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Book Synopsis Affinity Chromatography by : Sameh Magdeldin

Download or read book Affinity Chromatography written by Sameh Magdeldin and published by BoD – Books on Demand. This book was released on 2012-03-21 with total page 360 pages. Available in PDF, EPUB and Kindle. Book excerpt: Most will agree that one major achievement in the bio-separation techniques is affinity chromatography. This coined terminology covers a myriad of separation approaches that relies mainly on reversible adsorption of biomolecules through biospecific interactions on the ligand. Within this book, the authors tried to deliver for you simplified fundamentals of affinity chromatography together with exemplarily applications of this versatile technique. We have always been endeavor to keep the contents of the book crisp and easily comprehensive, hoping that this book will receive an overwhelming interest, deliver benefits and valuable information to the readers.

Technologies for Biochemical Conversion of Biomass

Author : Hongzhang Chen
Publisher : Academic Press
ISBN 13 : 0128025948
Total Pages : 292 pages
Book Rating : 4.1/5 (28 download)

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Book Synopsis Technologies for Biochemical Conversion of Biomass by : Hongzhang Chen

Download or read book Technologies for Biochemical Conversion of Biomass written by Hongzhang Chen and published by Academic Press. This book was released on 2016-12-14 with total page 292 pages. Available in PDF, EPUB and Kindle. Book excerpt: Technologies for Biochemical Conversion of Biomass introduces biomass biochemical conversion technology, including the pretreatment platform, enzyme platform, cell refining platform, sugar platform, fermentation platform, and post-treatment platform. Readers will find a systematic treatment, not only of the basics of biomass biochemical conversion and the introduction of each strategy, but also of the current advances of research in this area. Researchers will find the key problems in each technology platform for biomass biochemical conversion identified and solutions offered. This valuable reference book features new scientific research and the related industrial application of biomass biochemical conversion technology as the main content, and then systematically introduces the basic principles and applications of biomass biochemical conversion technology. Combines descriptions of these technologies to provide strategies and a platform for biochemical conversion in terms of basic knowledge, research advances, and key problems Summarizes models of biomass biochemical conversion for multiple products Presents products of biomass biochemical conversion from C1 to C10

Protein Purification

Author : Jan-Christer Janson
Publisher : John Wiley & Sons
ISBN 13 : 9780471186267
Total Pages : 712 pages
Book Rating : 4.1/5 (862 download)

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Book Synopsis Protein Purification by : Jan-Christer Janson

Download or read book Protein Purification written by Jan-Christer Janson and published by John Wiley & Sons. This book was released on 1998 with total page 712 pages. Available in PDF, EPUB and Kindle. Book excerpt: This is a state-of-the-art sourcebook on modern high-resolution biochemical separation techniques for proteins. It contains all the basic theory and principles used in protein chromatography and electrophoresis.

Applied Biophysics for Drug Discovery

Author : Donald Huddler
Publisher : John Wiley & Sons
ISBN 13 : 111909948X
Total Pages : 148 pages
Book Rating : 4.1/5 (19 download)

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Book Synopsis Applied Biophysics for Drug Discovery by : Donald Huddler

Download or read book Applied Biophysics for Drug Discovery written by Donald Huddler and published by John Wiley & Sons. This book was released on 2017-10-02 with total page 148 pages. Available in PDF, EPUB and Kindle. Book excerpt: Applied Biophysics for Drug Discovery is a guide to new techniques and approaches to identifying and characterizing small molecules in early drug discovery. Biophysical methods are reasserting their utility in drug discovery and through a combination of the rise of fragment-based drug discovery and an increased focus on more nuanced characterisation of small molecule binding, these methods are playing an increasing role in discovery campaigns. This text emphasizes practical considerations for selecting and deploying core biophysical method, including but not limited to ITC, SPR, and both ligand-detected and protein-detected NMR. Topics covered include: • Design considerations in biophysical-based lead screening • Thermodynamic characterization of protein-compound interactions • Characterizing targets and screening reagents with HDX-MS • Microscale thermophoresis methods (MST) • Screening with Weak Affinity Chromatography • Methods to assess compound residence time • 1D-NMR methods for hit identification • Protein-based NMR methods for SAR development • Industry case studies integrating multiple biophysical methods This text is ideal for academic investigators and industry scientists planning hit characterization campaigns or designing and optimizing screening strategies.