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Author : Edwin Louis Gump
Publisher :
ISBN 13 :
Total Pages : 546 pages
Book Rating : 4.3/5 (121 download)
Download or read book Analysis of Proteins and Peptides Using Microcolumn Analytical Methods written by Edwin Louis Gump and published by . This book was released on 1998 with total page 546 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author : Guodong Chen
Publisher : Springer Science & Business Media
ISBN 13 : 1441978623
Total Pages : 408 pages
Book Rating : 4.4/5 (419 download)
Download or read book Characterization of Protein Therapeutics using Mass Spectrometry written by Guodong Chen and published by Springer Science & Business Media. This book was released on 2014-07-08 with total page 408 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book highlights current approaches and future trends in the use of mass spectrometry to characterize protein therapies. As one of the most frequently utilized analytical techniques in pharmaceutical research and development, mass spectrometry has been widely used in the characterization of protein therapeutics due to its analytical sensitivity, selectivity, and specificity. This book begins with an overview of mass spectrometry techniques as related to the analysis of protein therapeutics, structural identification strategies, quantitative approaches, followed by studies involving characterization of process related protein drug impurities/degradants, metabolites, higher order structures of protein therapeutics. Both general practitioners in pharmaceutical research and specialists in analytical sciences will benefit from this book that details step-by-step approaches and new strategies to solve challenging problems related to protein therapeutics research and development.
Author : Jennie R. Lill
Publisher : John Wiley & Sons
ISBN 13 : 1119053102
Total Pages : 378 pages
Book Rating : 4.1/5 (19 download)
Download or read book Analytical Characterization of Biotherapeutics written by Jennie R. Lill and published by John Wiley & Sons. This book was released on 2017-08-14 with total page 378 pages. Available in PDF, EPUB and Kindle. Book excerpt: The definitive guide to the myriad analytical techniques available to scientists involved in biotherapeutics research Analytical Characterization of Biotherapeutics covers all current and emerging analytical tools and techniques used for the characterization of therapeutic proteins and antigen reagents. From basic recombinant antigen and antibody characterization, to complex analyses for increasingly complex molecular designs, the book explores the history of the analysis techniques and offers valuable insights into the most important emerging analytical solutions. In addition, it frames critical questions warranting attention in the design and delivery of a therapeutic protein, exposes analytical challenges that may occur when characterizing these molecules, and presents a number of tested solutions. The first single-volume guide of its kind, Analytical Characterization of Biotherapeutics brings together contributions from scientists at the leading edge of biotherapeutics research and manufacturing. Key topics covered in-depth include the structural characterization of recombinant proteins and antibodies, antibody de novo sequencing, characterization of antibody drug conjugates, characterization of bi-specific or other hybrid molecules, characterization of manufacturing host-cell contaminant proteins, analytical tools for biologics molecular assessment, and more. Each chapter is written by a recognized expert or experts in their field who discuss current and cutting edge approaches to fully characterizing biotherapeutic proteins and antigen reagents Covers the full range of characterization strategies for large molecule based therapeutics Provides an up-to-date account of the latest approaches used for large molecule characterization Chapters cover the background needed to understand the challenges at hand, solutions to characterize these large molecules, and a summary of emerging options for analytical characterization Analytical Characterization of Biotherapeutics is an up-to-date resource for analytical scientists, biologists, and mass spectrometrists involved in the analysis of biomolecules, as well as scientists employed in the pharmaceuticals and biotechnology industries. Graduate students in biology and analytical science, and their instructors will find it to be fascinating and instructive supplementary reading.
Author : Marie-Isabel Aguilar
Publisher : Springer Science & Business Media
ISBN 13 : 1592597424
Total Pages : 411 pages
Book Rating : 4.5/5 (925 download)
Download or read book HPLC of Peptides and Proteins written by Marie-Isabel Aguilar and published by Springer Science & Business Media. This book was released on 2008-02-03 with total page 411 pages. Available in PDF, EPUB and Kindle. Book excerpt: The introduction of high-performance liquid chromatography (HPLC) to the analysis of peptides and proteins some 25 years ago revolutionized the biological sciences by enabling the rapid and sensitive analysis of peptide and protein structure through the exquisite speed, sensitivity, and resolution that can be easily obtained. Today, HPLC in its various modes has become the pivotal technique in the characterization of peptides and proteins and currently plays a critical role in both our understanding of biological processes and in the development of peptide- and protein-based pharmaceuticals. The number of applications of HPLC in peptide and protein purification continues to expand at an extremely rapid rate. Solid-phase peptide synthesis and recombinant DNA techniques have allowed the production of large quantities of peptides and proteins that need to be highly purified. HPLC techniques are also used extensively in the isolation and characterization of novel proteins that will become increasingly important in the postgenomic age. The design of multidimensional purification schemes to achieve high levels of product purity further demonstrates the power of HPLC techniques not only in the characterization of cellular events, but also in the production of pepti- and protein-based therapeutics. HPLC continues to be at the heart of the analytical techniques with which scientists in both academia and in industry must arm themselves to be able to fully characterize the identity, purity, and potency of peptides and proteins.
Author : A.L. Burlingame
Publisher : Gulf Professional Publishing
ISBN 13 : 9780121828073
Total Pages : 520 pages
Book Rating : 4.8/5 (28 download)
Download or read book Biological Mass Spectrometry written by A.L. Burlingame and published by Gulf Professional Publishing. This book was released on 2005-11-28 with total page 520 pages. Available in PDF, EPUB and Kindle. Book excerpt: Describes and integrates the techniques of many advances in both chromatographic and mass spectrometric technologies. This book also covers various biophysical applications, such as H/D exchange for study of conformations, protein-protein and protein-metal and ligand interactions. It also describes atto-to-zepto-mole quantitation of 14C and 3H.
Author : U A Th Brinkman
Publisher : Elsevier
ISBN 13 : 1483274616
Total Pages : 405 pages
Book Rating : 4.4/5 (832 download)
Download or read book TRAC: Trends in Analytical Chemistry written by U A Th Brinkman and published by Elsevier. This book was released on 2013-09-24 with total page 405 pages. Available in PDF, EPUB and Kindle. Book excerpt: TRAC: Trends in Analytical Chemistry, Volume 8 provides information pertinent to the trends in the field of analytical chemistry. This book presents a variety of topics related to analytical chemistry, including protein purification, biotechnology, Raman spectroscopy in pharmaceutical field, electrokinetic chromatography, and flow injection analysis. Organized into 50 chapters, this volume begins with an overview of scientometric investigations that enable the quantitative study of the evolution of its various components and can thereby uncover how information is utilized to diffuse and generate knowledge. This text then discusses the economic significance of sensing and control as being the main factors in determining process economics and in offering products and business opportunities. Other chapters consider the important relationship between Raman spectroscopy and other analytical methods. This book discusses as well the interfaces between a gas chromatograph and a Fourier transform infrared spectrometer. The final chapter deals with chemometrics routines. This book is a valuable resource for analytical chemists, and biochemists.
Author : M. Zouhair Atassi
Publisher : Springer Science & Business Media
ISBN 13 : 148991031X
Total Pages : 522 pages
Book Rating : 4.4/5 (899 download)
Download or read book Methods in Protein Structure Analysis written by M. Zouhair Atassi and published by Springer Science & Business Media. This book was released on 2013-06-29 with total page 522 pages. Available in PDF, EPUB and Kindle. Book excerpt: The MPSA international conference is held in a different country every two years. It is devoted to methods of determining protein structure with emphasis on chemistry and sequence analysis. Until the ninth conference, MPSA was an acronym for Methods in Protein Sequence Analysis. To give the conference more flexibility and breadth, the Scientific Advisory Committee of the lOth MPSA decided to change the name to Methods in Protein Structure Analysis; however, the emphasis remains on "methods" and on "chemistry. " In fact, this is the only major conference that is devoted to methods. The MPSA conference is truly international, a fact clearly reflected by the composi tion of its Scientific Advisory Committee. The Scientific Advisory Committee oversees the scientific direction of the MPSA and elects the chairman of the conference. Members of the committee are elected by active members, based on scientific standing and activity. The chairman, subject to approval of the Scientific Advisory Committee, appoints the Organizing Committee. It is this latter committee that puts the conference together. The lectures of the MPSA have traditionally been published in a special proceedings issue. This is different from, and more detailed than, the special MPSA issue of the Journal of Protein Chemistry in which only a brief description of the talks is given in short papers and abstracts. In the I Oth MPSA, about half the talks are by invited speakers and the remainder were selected from submitted short papers and abstracts.
Author : James P. Landers
Publisher : CRC Press
ISBN 13 : 9780849324987
Total Pages : 918 pages
Book Rating : 4.3/5 (249 download)
Download or read book Handbook of Capillary Electrophoresis, Second Edition written by James P. Landers and published by CRC Press. This book was released on 1996-12-23 with total page 918 pages. Available in PDF, EPUB and Kindle. Book excerpt: Because new information was discovered at an incredible rate since the publication of the successful first edition of this Handbook, this fully updated second edition covers all areas of interest in the field of capillary electrophoresis (CE). A relatively new technology, CE is a principle method for studying the physicochemical properties of proteins, peptides, and other macromolecules. Where applicable, the 30 chapters provide basic underlying theories as well as application-oriented aspects of each technique.Keep up with all the developments in this growing field with the Handbook of Capillary Electrophoresis, Second Edition - a complete guide to the fundamentals of CE and the latest research. The chapters are organized into five units: Modes: Presents a theoretical development of the basic principles governing separation with several modes, including CEC, and discusses their practical aspects. Analyte: Applies CE to the analysis of a specific class of analytes, including organic and inorganic ions, pharmaceuticals, glycoconjugates, peptides, proteins, and DNA fragments. Fundamental Aspects of CE: Technique-oriented information for the practitioner, including the importance of the sample matrix, on-line preconcentration of samples, modes of detection, and specific aspects of CE data analysis. Applications of CE: Includes single cell analysis, CE in DNA sequencing, CE as a clinical diagnostic tool, identifying and quantifying drugs, and for characterizing interacting species. Specialized Aspects of CE: Discusses interfacing CE with mass spectrometry, high-volume throughput continuous CE, microchip CE, control of EOF, and much more. The Handbook of Capillary Electrophoresis, Second Edition, pulls together diverse areas and applications of CE, resulting in an excellent tool for scientists involved in biotechnology and clinical chemistry, as well as the pharmaceutical, bioscience, chemical, and instrument-manufacturing industries. With an applications-oriented focus, the handbook is also a superb manual for workshops, seminars, and graduate courses in separation science.
Author : Rizwan Ahmad
Publisher : BoD – Books on Demand
ISBN 13 : 9533078316
Total Pages : 236 pages
Book Rating : 4.5/5 (33 download)
Download or read book Protein Purification written by Rizwan Ahmad and published by BoD – Books on Demand. This book was released on 2012-01-20 with total page 236 pages. Available in PDF, EPUB and Kindle. Book excerpt: The current volume entitled Protein Purification is designed to facilitate rapid access to valuable information about various methodologies. It aims as well to provide an overview of state-of-art techniques for the purification, analysis and quantification of proteins in complex samples using different enrichment strategies.
Author :
Publisher :
ISBN 13 :
Total Pages : 924 pages
Book Rating : 4.F/5 ( download)
Download or read book Dissertation Abstracts International written by and published by . This book was released on 2007 with total page 924 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author : Albert van den Berg
Publisher : Springer Science & Business Media
ISBN 13 : 9401722641
Total Pages : 639 pages
Book Rating : 4.4/5 (17 download)
Download or read book Micro Total Analysis Systems 2000 written by Albert van den Berg and published by Springer Science & Business Media. This book was released on 2013-11-11 with total page 639 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume contains the proceedings of the fourth international symposium on Micro Total Analysis Systems (muTAS 2000). Cutting-edge research of all invited and contributed papers presented by the world’s leading muTAS groups provides the state of the art of this electrifying, multidisciplinary field.
Author : John R. Griffiths
Publisher : John Wiley & Sons
ISBN 13 : 1119045851
Total Pages : 414 pages
Book Rating : 4.1/5 (19 download)
Download or read book Analysis of Protein Post-Translational Modifications by Mass Spectrometry written by John R. Griffiths and published by John Wiley & Sons. This book was released on 2016-11-07 with total page 414 pages. Available in PDF, EPUB and Kindle. Book excerpt: Covers all major modifications, including phosphorylation, glycosylation, acetylation, ubiquitination, sulfonation and and glycation Discussion of the chemistry behind each modification, along with key methods and references Contributions from some of the leading researchers in the field A valuable reference source for all laboratories undertaking proteomics, mass spectrometry and post-translational modification research
Author : Hassan Y. Aboul-Enein
Publisher : CRC Press
ISBN 13 : 1000110400
Total Pages : 479 pages
Book Rating : 4.0/5 (1 download)
Download or read book Analytical and Preparative Separation Methods of Biomacromolecules written by Hassan Y. Aboul-Enein and published by CRC Press. This book was released on 2020-10-14 with total page 479 pages. Available in PDF, EPUB and Kindle. Book excerpt: Reports up-to-date research developments on purifying and isolation large organic molecules. The text provides information on high-performance liquid chromatography and capillary electrophoresis (CE) as tools for analyzing biomacromolecules and developing new biochemical and medicinal compounds. It applies biochemical separation technology to the study of macromolecules such as proteins, polysaccharides, nucleic acids and more.
Author :
Publisher :
ISBN 13 :
Total Pages : 1436 pages
Book Rating : 4.3/5 ( download)
Download or read book Research Awards Index written by and published by . This book was released on 1985 with total page 1436 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author :
Publisher : Elsevier
ISBN 13 : 0080542263
Total Pages : 609 pages
Book Rating : 4.0/5 (85 download)
Download or read book Techniques in Protein Chemistry written by and published by Elsevier. This book was released on 1995-04-13 with total page 609 pages. Available in PDF, EPUB and Kindle. Book excerpt: Techniques in Protein Chemistry VI, an invaluable bench-top reference source for protein chemists, highlights current methods in the following areas: - Protein sequencing and amino acid analysis - Mass spectral analysis of peptides and proteins - Posttranslational processing - High-sensitivity protein and peptide separations - Protein folding and NMR - Analysis of protein interactions - Protein design and engineeringTechniques in Protein Chemistry VI, an invaluable bench-top reference source for protein chemists, highlights current methods in the following areas: - Protein sequencing and amino acid analysis - Mass spectral analysis of peptides and proteins - Posttranslational processing - High-sensitivity protein and peptide separations - Protein folding and NMR - Analysis of protein interactions - Protein design and engineering
Author : A. Berthod
Publisher :
ISBN 13 :
Total Pages : 432 pages
Book Rating : 4.:/5 (89 download)
Download or read book Countercurrent Chromatography written by A. Berthod and published by . This book was released on 2002-10-10 with total page 432 pages. Available in PDF, EPUB and Kindle. Book excerpt: Countercurrent chromatography (CCC) is a separation technique in which the stationary phase is a liquid. The mobile phase is also a liquid, so biphasic liquid systems with at least two solvents are used. Centrifugal fields are used to hold the liquid stationary phase while pushing the liquid mobile phase through it. This comprehensive reference covers recent advancements in the two types of CCC machines: the high speed CCCs without rotary seals and with coiled spools and centrifugal partition chromatographs (CPC) with rotary seals and interconnected channels. Written by leading international experts in the CCC field, the book focuses on the liquid nature of the stationary phase: giving newcomers the basis to do CCC efficiently and rapidly; explaining the art of obtaining a biphasic liquid system; describing the flow patterns in both CPC and high speed CCC machines; showing possible other uses of a liquid stationary phase; presenting a wealth of applications in the separation of organic, pharmaceutical and inorganic mixtures; and demonstrating that even supercritical fluids can be used in CCC.
Author : Leo M.L. Nollet
Publisher : CRC Press
ISBN 13 : 1439830843
Total Pages : 1081 pages
Book Rating : 4.4/5 (398 download)
Download or read book Food Analysis by HPLC, Third Edition written by Leo M.L. Nollet and published by CRC Press. This book was released on 2012-11-16 with total page 1081 pages. Available in PDF, EPUB and Kindle. Book excerpt: For food scientists, high-performance liquid chromatography (HPLC) is a powerful tool for product composition testing and assuring product quality. Since the last edition of this volume was published, great strides have been made in HPLC analysis techniques—with particular attention given to miniaturization, automatization, and green chemistry. Thoroughly updated and revised, Food Analysis by HPLC, Third Edition offers practical and immediately applicable information on all major topics of food components analyzable by HPLC. Maintaining the rigorous standards that made the previous editions so successful and lauded by food scientists worldwide, this third edition examines: Recent trends in HPLC HPLC separation techniques for amino acids, peptides, proteins, neutral lipids, phospholipids, carbohydrates, alcohols, vitamins, and organic acids HPLC analysis techniques for sweeteners, colorants, preservatives, and antioxidants HPLC determinations of residues of mycotoxins, antimicrobials, carbamates, organochlorines, organophosphates, herbicides, fungicides, and nitrosamines HPLC determinations of residues of growth promoters, endocrine disrupting chemicals, polycyclic aromatic hydrocarbons, polychlorinated biphenyls, and dioxins HPLC applications for the analysis of phenolic compounds, anthocyanins, betalains, organic bases, anions, and cations Presenting specific and practical applications to food chemistry, the contributors provide detailed and systematic instructions on sample preparation and separation conditions. The book is an essential reference for those in the fields of chromatography, analytical chemistry, and, especially, food chemistry and food technology.
