Author : Dr. D. A. Dhale
Publisher : Book Saga Publications
ISBN 13 : 9395470429
Total Pages : 316 pages
Book Rating : 4.3/5 (954 download)
Book Synopsis Advanced Techniques in Plant Sciences by : Dr. D. A. Dhale
Download or read book Advanced Techniques in Plant Sciences written by Dr. D. A. Dhale and published by Book Saga Publications . This book was released on 2023-05-28 with total page 316 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book, ‘Advanced Techniques in Plant Sciences’ includes basic and advanced techniques like microscopy, polarizing microscopy, transmission and scanning electron microscopes, confocal microscopy, phase contrast microscopy, photomicrography, digital imaging, and microscopic measurements. The Spectroscopic techniques contain Colorimeter, Spectroscopic techniques, Ultraviolet-visible spectroscopy; Infrared spectroscopy, Nuclear magnetic resonance spectroscopy; Basic NMR techniques, NMR Imaging Principles, Electron Spin Resonance (ESR) Spectroscopy, Atomic Absorption Spectroscopy (AAS); Mass spectrometry, Flame photometer, etc. In histochemical studies, various methods include proteins, carbohydrates, lipids, and enzymes; how to prepare the macrolides; and the different methods of preparing the slides, which include Along with this, you will also study whole mounts. Also, about the sectioning of plant specimens, free hand, microtome, rotary microtome sectioning, types of microtome, and methods of serial sectioning. Maceration, Gel electrophoresis, PAGE, SDS-PAGE, Agarose gel electrophoresis; isoelectric focusing, and 2D electrophoresis Blotting Techniques: Principles and Techniques of Southern, Northern, and Western Blotting Techniques and Southwestern Blotting. Centrifugation techniques, Types of Centrifugation, Ultracentrifugation, Concept of Partition Coefficient; Chromatography, Techniques by chromatographic bed shape, Paper Chromatography, Double-way paper chromatography, Planar chromatography, Thin layer chromatography (TLC), High-performance liquid chromatography (HPLC)- Partition chromatography, Normal-phase chromatography, Displacement chromatography, Reversed-phase chromatography (RPC), Size-exclusion chromatography, Ion-exchange chromatography, Bioaffinity chromatography, Aqueous normal-phase chromatography, Isocratic flow, and gradient elution; High-Performance Thin-Layer Chromatography (HPTLC), Gas chromatography (GC), Flash column chromatography, Column chromatography, Ion chromatography, Gel-filtration chromatography or Size-exclusion chromatography, Pyrolysis gas chromatography, Countercurrent chromatography.