Read Books Online and Download eBooks, EPub, PDF, Mobi, Kindle, Text Full Free.
Download A Study Of Sulfhydryl Groups And Disulfide Bonds In Proteins full books in PDF, epub, and Kindle. Read online A Study Of Sulfhydryl Groups And Disulfide Bonds In Proteins ebook anywhere anytime directly on your device. Fast Download speed and no annoying ads. We cannot guarantee that every ebooks is available!
Author : Eleanor Groeniger
Publisher :
ISBN 13 :
Total Pages : 94 pages
Book Rating : 4.:/5 (978 download)
Download or read book A Study of Sulfhydryl Groups and Disulfide Bonds in Proteins written by Eleanor Groeniger and published by . This book was released on 1963 with total page 94 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author : Yu M. Torchinskii
Publisher : Springer Science & Business Media
ISBN 13 : 1475701276
Total Pages : 282 pages
Book Rating : 4.4/5 (757 download)
Download or read book Sulfhydryl and Disulfide Groups of Proteins written by Yu M. Torchinskii and published by Springer Science & Business Media. This book was released on 2013-11-22 with total page 282 pages. Available in PDF, EPUB and Kindle. Book excerpt: (Written for the original Russian edition) Out of all the functional groups of protein molecules that be long to the side chains of amino acid residues, the sulfhydryl or thiol (SH) groups of cysteine residues and the disulfide (S-S) groups of cystine have for long attracted particular attention from chemists and biochemists. This is due, on the one hand, to the high chemical reactivity of these groups, which enter into many, and often very characteristic and selective, reactions with several types of compound. On the other hand, this attention is connected with the great significance of SH and S-S groups for the specific functions of a number of enzymes, hormones, and other biological ly active proteins, and consequently for the normal course of many physiological processes. The varied functions of sulfhydryl and disulfide groups in en zymes are particularly interesting; their role can consist of bind ing substrates and cofactors, in direct participation in the catalytic act, or in maintaining (in ways not so far sufficiently studied) the native, catalytically active conformation of the protein. It is entirely natural that the detailed study of the chemical behavior and biological role of these sulfur-containing groups in proteins (and in such low-molecular cofactors as glutathione, co enzyme A, lipoiC acid, etc. ) has, in addition to its academic, gen eral biological interest, great Significance for many applied branches of medicine and biology (toxicology, radiobiology, diagnostics and therapeutics, biochemical engineering, plant growing, etc. ).
Author : Mendel Friedman
Publisher : Pergamon
ISBN 13 :
Total Pages : 500 pages
Book Rating : 4.3/5 (91 download)
Download or read book The Chemistry and Biochemistry of the Sulfhydryl Group in Amino Acids, Peptides, and Proteins written by Mendel Friedman and published by Pergamon. This book was released on 1973 with total page 500 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author : Reinhold Benesch
Publisher : Elsevier
ISBN 13 : 0323148689
Total Pages : 482 pages
Book Rating : 4.3/5 (231 download)
Download or read book Sulfur in Proteins written by Reinhold Benesch and published by Elsevier. This book was released on 2012-12-02 with total page 482 pages. Available in PDF, EPUB and Kindle. Book excerpt: Sulfur in Proteins is a collection of papers presented at the 1958 Symposium on Sulfur in Proteins, held in Falmouth, Massachusetts. The symposium primary aims to explore the varied and unusual roles of –S–S– and –SH groups in proteins. This book is organized into eight chapters that cover a wide variety of proteins, including serum, iron, copper, and muscle proteins, as well as enzymes and viruses. The opening chapter describes the various protein reactions involving sulfur, emphasizing the chemical modification of thiol and disulfide groups. Considerable chapters are devoted to the chemical properties, biogenesis, reactivity, and oxygenation of specific proteins. A chapter highlights some aspects of protein structure in relation to the role of –S–S– and –SH groups in enzymic catalysis. This chapter also describes the properties of sulfhydryl groups in yeast alcohol dehydrogenase. The final chapters examine the role of sulfur groups in several virus proteins and in cell division. This work will be of great value to protein scientists and researchers.
Author : Greg T. Hermanson
Publisher : Academic Press
ISBN 13 : 0080568726
Total Pages : 1233 pages
Book Rating : 4.0/5 (85 download)
Download or read book Bioconjugate Techniques written by Greg T. Hermanson and published by Academic Press. This book was released on 2010-07-26 with total page 1233 pages. Available in PDF, EPUB and Kindle. Book excerpt: Bioconjugate Techniques, 2nd Edition, is the essential guide to the modification and cross linking of biomolecules for use in research, diagnostics, and therapeutics. It provides highly detailed information on the chemistry, reagent systems, and practical applications for creating labeled or conjugate molecules. It also describes dozens of reactions with details on hundreds of commercially available reagents and the use of these reagents for modifying or cross linking peptides and proteins, sugars and polysaccharides, nucleic acids and oligonucleotides, lipids, and synthetic polymers. A one-stop source for proven methods and protocols for synthesizing bioconjugates in the lab Step-by-step presentation makes the book an ideal source for researchers who are less familiar with the synthesis of bioconjugates More than 600 figures that visually describe the complex reactions associated with the synthesis of bioconjugates Includes entirely new chapters on the latest areas in the field of bioconjugation as follows: Microparticles and nanoparticlesSilane coupling agentsDendrimers and dendronsChemoselective ligationQuantum dotsLanthanide chelatesCyanine dyesDiscrete PEG compoundsBuckyballs,fullerenes, and carbon nanotubesMass tags and isotope tagsBioconjugation in the study of protein interactions
Author : Brookhaven National Laboratory
Publisher :
ISBN 13 :
Total Pages : 296 pages
Book Rating : 4.:/5 (44 download)
Download or read book Protein Structure and Function written by Brookhaven National Laboratory and published by . This book was released on 1960 with total page 296 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author : R. S. Asquith
Publisher : Springer Science & Business Media
ISBN 13 : 1461341094
Total Pages : 431 pages
Book Rating : 4.4/5 (613 download)
Download or read book Chemistry of Natural Protein Fibers written by R. S. Asquith and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 431 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume arose originally from the complaints of the editor's students, both undergraduate and postgraduate, that there was no modern book on protein fibers which told enough about protein science and chemical tech nologies related to fibers. By and large this is probably a reasonable cri de coeur. The undergraduate on a technological course, lacking information on the basic scientific techniques used to carry out the research on which his fiber technology is based, can find it difficult to obtain this information. The pure science undergraduate often lacks knowledge of the application of these techniques in protein fiber technology. The young graduates, com mencing research related to some aspect of protein fibers, are drawn from a wide range of scientific disciplines, having been trained as biochemists, chemists, physicists, technologists, and histologists, to name but a few. Generally these new research workers pass through a preliminary "lost" period in which they have to evaluate their background in relation to the wide and differing fields of research in protein fiber science to which they are now exposed. As time goes on they then either develop a wide knowledge covering science and technology or remain in a specific part of their original discipline, with a narrow knowledge of its application in the field of the research degree they are taking.
Author : Rowen J. Y. Chang
Publisher : Springer Science & Business Media
ISBN 13 : 1441972730
Total Pages : 290 pages
Book Rating : 4.4/5 (419 download)
Download or read book Folding of Disulfide Proteins written by Rowen J. Y. Chang and published by Springer Science & Business Media. This book was released on 2011-08-12 with total page 290 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book aims to cover the knowledge of protein folding accumulated from studies of disulfide-containing proteins, including methodologies, folding pathways, and folding mechanism of numerous extensively characterized disulfide proteins. Folding of Disulfide Proteins will be valuable supplementary reading for general biochemistry, biophysics, molecular biology, and cellular biology courses for graduate and undergraduate students. This book can also be used for specialized graduate-level biochemistry, biophysics, and molecular biology courses dedicated to protein folding as well as related biological problems and diseases. Will also be of interest to everybody interested in problems related to protein folding, and anyone who is interested in understanding the mechanism of protein misfolding and protein misfolding-related diseases.
Author : Luis Moroder
Publisher : Royal Society of Chemistry
ISBN 13 : 1847559263
Total Pages : 452 pages
Book Rating : 4.8/5 (475 download)
Download or read book Oxidative Folding of Peptides and Proteins written by Luis Moroder and published by Royal Society of Chemistry. This book was released on 2008-12-10 with total page 452 pages. Available in PDF, EPUB and Kindle. Book excerpt: The formation of disulfide bonds is probably the most influential modification of peptides and proteins. An elaborate set of cellular machinery exists to catalyze and guide this process. In recent years, significant developments have been made in both our understanding of the in vivo situation and the in vitro manipulation of disulfide bonds. This is the first monograph to provide a comprehensive overview of this exciting and rapidly developing area. It offers in-depth insights into the mechanisms of in vivo and in vitro oxidative folding of proteins as well as mono- and multiple-stranded peptides. Procedures applied for laboratory and industrial purposes are also discussed by top experts in the field. The book describes the enzymes involved in the correct oxidative folding of cysteine-containing proteins in prokaryotes and eukaryotes. It then goes on to discuss the mimicking of these enzymes for successful in vitro folding of proteins (including synthetic replicates) and to deal with important issues concerning cysteine-rich peptides. The ability of natural bioactive peptides to fold correctly, and in high yields, to form defined structural motifs using cysteine sequence patterns is still puzzling. With this in mind, synthetic procedures for establishing native cysteine frameworks are discussed using selected examples, such as the potential of selenocysteines. The biotechnological and pharmaceutical relevance of proteins, peptides, their variants and synthetic replicates is continuously increasing. Consequently, this book is invaluable for peptide and protein chemists involved in related research and production.
Author : M. Zouhair Atassi
Publisher : Springer Science & Business Media
ISBN 13 : 1461341906
Total Pages : 495 pages
Book Rating : 4.4/5 (613 download)
Download or read book Immunochemistry of Proteins written by M. Zouhair Atassi and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 495 pages. Available in PDF, EPUB and Kindle. Book excerpt: The structural features responsible for the immunogenicity of certain parts of native protein molecules have been of interest to immunochemists and protein chemists for over three decades. Following the early work of Land steiner in 1942, which showed that peptide fragments from silk fibroin exhibited an inhibitory activity toward the reaction of the protein with its antibodies, fragments from many other protein systems have been isolated and studied. However, no concerted effort was (or could be) devoted to the elucidation of the complete antigenic structure of a protein. In order for these endeavors to be successful and meaningful, knowledge of both the amino acid sequence and the detailed three-dimensional structure of the protein is necessary. Such information was not available for a protein until early in the 1960s. This and the fact that protein chemistry was not in fact sufficiently developed early in the 1960s to enable the successful completion of the entire antigenic structure of a protein were major contributing factors for the slow progress in this field. Determination of the antigenic structures of proteins therefore posed a chemical challenge of enormous proportions. For these reasons, many investigators diverted their attention to study of the immunochemistry of homo- or mixed amino acid polymers in the hope that the information derived from these systems might prove useful in the understanding of the immunochemistry of proteins.
Author : Pooja Rajeshbhai Ramanuj
Publisher :
ISBN 13 :
Total Pages : 64 pages
Book Rating : 4.:/5 (132 download)
Download or read book Enrichment and Identification of Disulfide Bonded Peptides by Tandem Mass Spectrometry written by Pooja Rajeshbhai Ramanuj and published by . This book was released on 2017 with total page 64 pages. Available in PDF, EPUB and Kindle. Book excerpt: Proteins are Comprised of different amino acids which can undergo various modifications known as post-translational modifications (PTMs). Amino acid's side chains are the most common sites for the PTMs. Cysteine side chain can undergo several PTMs and one very common one is disulfide bond formation. Disulfide bond aids in maintaining protein tertiary structures, its identification is an inevitable aspect in studying protein functions. Furthermore, disulfide bonds are potential targets to develop new protein therapeutics for life-threatening diseases such as cancer. Literature surveys revealed several methods to identify disulfide bonds, which require either high quality sample or are highly laborious. Moreover, unexpected folding patterns in protein structure limits the use of data software in disulfide identification. In this study, a straightforward and novel method was developed for confident identification of cysteine residues involved in disulfide bond formation. In order to develop a robust strategy for mapping disulfide bonds in proteins, we developed a chemical strategy. First, we reduced the disulfide bonds to two -SH and subsequently reacted with a reagent called 2, 3- dibromomaleimide (DBM). The maleimide reacted with sulfhydryl bond of cysteine amino acid and substituted the disulfide bonds. Cysteine sulfur was converted to sulfoxide by mild oxidation to make this bond low energy tandem mass spectrometry cleavable. Due to cleavage, this produced two different peptides peaks in the mass spectra so disulfide-bonded peptides can be sequenced as linear modified peptides using tandem mass spectrometry. Furthermore, to enrich the disulfide bonds, we added a biotin group in the dibromomaleimide. Biotin is a small molecule, which has strong interaction with a protein avidin. After reduction of disulfide bonds in peptides, we used a similar dibromomaleimide reagent with a biotin group incorporated in it. After that peptide were enriched using avidin immobilized agarose or magnetic beads. After washing the non-binders, the disulfide-bonded peptide was eluted from the beads and peptide sequence was analyzed using liquid chromatography and tandem mass spectrometry. We tested our approach with standard disulfide containing peptides, somatostatin and a protein lysozyme. Efficient labeling was observed in standard peptides and we have identified several disulfide bond scrambling in lysozyme.In comparison to conventional approaches, our method has three major advantages: (I) reduced sample complexity (II) precise mapping of disulfide bonds (III) faster and easy MS data analysis. Although, there are certain challenges involving oxidation study using biotin dibromomaleimide (BDBM), this method highly enhances the confidence identification of disulfide bonds. We believe this strategy could be an efficient way to enrich and analyze disulfide bonds in proteins.
Author : Shan S. Wong
Publisher : CRC Press
ISBN 13 : 9780849358869
Total Pages : 360 pages
Book Rating : 4.3/5 (588 download)
Download or read book Chemistry of Protein Conjugation and Cross-Linking written by Shan S. Wong and published by CRC Press. This book was released on 1991-06-18 with total page 360 pages. Available in PDF, EPUB and Kindle. Book excerpt: Chemical cross-linking reagents have attained great practical use in industry as well as in basic research, and an understanding of their fundamental principles of reaction is paramount to their applications. With broad coverage of the development and application of these reagents, Chemistry of Protein Conjugation and Cross-Linking discusses the mechanism of reaction and allows you to put the theory into practice. The book offers an explanation of the underlying mechanism of chemical modification, surveys all the bifunctional reagents used in bioconjugation and cross-linking, and provides a review of practical applications of these reagents in various areas of biochemistry, molecular biology, biotechnology, nucleic acid chemistry, immunochemistry, and diagnostic and biomedical disciplines. It contains numerous examples and illustrations, plus step-by-step explanations to reaction procedures. It is an excellent introduction and a comprehensive reference about chemical modification.
Author : Bernd Gutte
Publisher : Elsevier
ISBN 13 : 0080538592
Total Pages : 527 pages
Book Rating : 4.0/5 (85 download)
Download or read book Peptides written by Bernd Gutte and published by Elsevier. This book was released on 1995-10-24 with total page 527 pages. Available in PDF, EPUB and Kindle. Book excerpt: In recent years, research has shown the importance of peptides in neuroscience, immunology, and cell biology. Active research programs worldwide are now engaged in developing peptide-based drugs and vaccines using modification of natural peptides and proteins, design of artificial peptides and peptide mimetics, and screening of peptide and phage libraries. In this comprehensive book, the authors discuss peptide synthesis and application within the context of their increasing importance to the pharmaceutical industry. Peptides: Synthesis, Structures, and Applications explores the broad growth of information in modern peptide synthetic methods and the structure-activity relationships of synthetic polypeptides. - The history of peptide chemistry - Amide formation, deprotection, and disulfide formation in peptide synthesis - Solid-phase peptide synthesis - a-helix formation by peptides in water - Stability and dynamics of peptide conformation - An overview of structure-function studies of peptide hormones - Neuropeptides:peptide and nonpeptide analogs - Reversible inhibitors of serine proteinases - Design of polypeptides - Current capabilities and future possibilities of soluble chemical combinatorial libraries - Epitope mapping with peptides - Synthesis and applications of branched peptides in immunological methods and vaccines
Author : Hans Neurath
Publisher : Elsevier
ISBN 13 : 0323161383
Total Pages : 683 pages
Book Rating : 4.3/5 (231 download)
Download or read book The Proteins Composition, Structure, and Function V1 written by Hans Neurath and published by Elsevier. This book was released on 2012-12-02 with total page 683 pages. Available in PDF, EPUB and Kindle. Book excerpt: The Proteins: Composition, Structure, and Function, Second Edition, Volume I explores the quantitative relationships between protein composition, structure, and function. This book is composed of six chapters that cover the rapid and fundamental advances in understanding protein chemistry. This book outlines first the quantitative procedures and various methods suitable for the determination of amino acids found as constituents of naturally occurring peptides and as free amino acids in tissues and body fluids. These topics are followed by a discussion on some of the aspects of peptide chemistry, which appear significant in relation to peptides possessing physiological activity. The next chapter considers protein synthesis that represents the sequences of chemical reactions whereby amino acids are assembled in biological systems to produce proteins. This volume also examines the correlation of structure with function; the mechanisms of control of protein biosynthesis; the exact role of intramolecular interactions in the determination of tertiary structure; and the colinearity of genetic "maps with amino acid sequences. A chapter describes the methods of analysis and reactions of sulfhydryl, disulfide, and thiol ester groups in proteins, as well as the evidence relating to the functions of these sulfur groups in proteins. The final chapter looks into the models and theories for the noncovalent bond interactions in proteins. This book is of value to organic chemists, biochemists, and researchers in the protein-related fields.
Author : Abba Kastin
Publisher : Elsevier
ISBN 13 : 0080463797
Total Pages : 1682 pages
Book Rating : 4.0/5 (84 download)
Download or read book Handbook of Biologically Active Peptides written by Abba Kastin and published by Elsevier. This book was released on 2011-04-28 with total page 1682 pages. Available in PDF, EPUB and Kindle. Book excerpt: Peptides play a crucial role in many physiological processes including actions as neurotransmitters, hormones, and antibiotics. Research has shown their importance in such fields as neuroscience, immunology, pharmacology, and cell biology. The Handbook of Biologically Active Peptides presents, for the first time, this tremendous body of knowledge in the field of biologically active peptides in one single reference. The section editors and contributors represent some of the most sophisticated and distinguished scientists working in basic sciences and clinical medicine. The Handbook of Biologically Active Peptides is a definitive, all-encompassing reference that will be indispensable for individuals ranging from peptide researchers, to biochemists, cell and molecular biologists, neuroscientists, pharmacologists, and to endocrinologists. Chapters are designed to be a source for workers in the field and will enable researchers working in a specific area to examine other related areas with which they would not ordinarily be familiar.*Chapters are designed to be a source for workers in the field and will enable researchers working in a specific area to examine other related areas that they would not ordinarily be familiar.*Fascinating relationships described in the book include the presence of some peptides originally found in frog skin that persist in the human human and brain where they can affect food intake and obesity.
Author : Mendel Friedman
Publisher : Springer Science & Business Media
ISBN 13 : 1468432826
Total Pages : 765 pages
Book Rating : 4.4/5 (684 download)
Download or read book Protein Crosslinking written by Mendel Friedman and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 765 pages. Available in PDF, EPUB and Kindle. Book excerpt: The word crosslinking implies durable combination of (usually large) distinct elements at specific places to create a new entity that has different properties as a result of the union. In the case of proteins, such crosslinking often results in important changes in chemical, functional, nutritional, and biomedical properties, besides physical properties simply related to molecular size and shape. (Nucleic acids, carbohydrates, and other biopolymers are correspondingly affected.) Since proteins are ubiquitous, the consequences of their crosslinking are widespread and often profound. Scientists from many disciplines including organic chemistry, bio chemistry, protein chemistry, food science, nutrition, radiation biology, pharmacology, physiology, medicine, and dentistry are, therefore, minutely interested in protein crosslinking reactions and their implications. Because protein crosslinking encompasses so many disciplines, in organizing the Symposium on Nutritional and Biochemical Conse quences of Protein Crosslinking sponsored by the Protein Subdivi sion of the Division of Agricultural and Food Chemistry of the American Chemical Society, I sought participants with the broadest possible range of interests, yet with a common concern for theore tical and practical aspects of protein crosslinking. An important function of a symposium is to catalyze progress by bringing together ideas and experiences needed for interaction among different, yet related disciplines. To my pleasant surprise, nearly everyone invited came to San Francisco to participate.
Download or read book Current Protocols in Protein Science written by and published by . This book was released on 1996 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
