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Author : Joseph Sambrook
Publisher :
ISBN 13 : 9780879695774
Total Pages : 0 pages
Book Rating : 4.6/5 (957 download)
Download or read book Molecular Cloning written by Joseph Sambrook and published by . This book was released on 2003 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author : Heather B. Miller
Publisher : Academic Press
ISBN 13 : 0123855454
Total Pages : 227 pages
Book Rating : 4.1/5 (238 download)
Download or read book Molecular Biology Techniques written by Heather B. Miller and published by Academic Press. This book was released on 2011-10-18 with total page 227 pages. Available in PDF, EPUB and Kindle. Book excerpt: This manual is an indispensable tool for introducing advanced undergraduates and beginning graduate students to the techniques of recombinant DNA technology, or gene cloning and expression. The techniques used in basic research and biotechnology laboratories are covered in detail. Students gain hands-on experience from start to finish in subcloning a gene into an expression vector, through purification of the recombinant protein. The third edition has been completely re-written, with new laboratory exercises and all new illustrations and text, designed for a typical 15-week semester, rather than a 4-week intensive course. The "project approach to experiments was maintained: students still follow a cloning project through to completion, culminating in the purification of recombinant protein. It takes advantage of the enhanced green fluorescent protein - students can actually visualize positive clones following IPTG induction. - Cover basic concepts and techniques used in molecular biology research labs - Student-tested labs proven successful in a real classroom laboratories - Exercises simulate a cloning project that would be performed in a real research lab - "Project" approach to experiments gives students an overview of the entire process - Prep-list appendix contains necessary recipes and catalog numbers, providing staff with detailed instructions
Author : Joseph Sambrook
Publisher : CSHL Press
ISBN 13 : 0879697717
Total Pages : 802 pages
Book Rating : 4.8/5 (796 download)
Download or read book The Condensed Protocols from Molecular Cloning written by Joseph Sambrook and published by CSHL Press. This book was released on 2006 with total page 802 pages. Available in PDF, EPUB and Kindle. Book excerpt: The Condensed Protocols From Molecular Cloning: A Laboratory Manualis a single–volume adaptation of the three–volume third edition of Molecular Cloning: A Laboratory Manual.This condensed book contains only the step–by–step portions of the protocols, accompanied by selected appendices from the world's best–selling manual of molecular biology techniques. Each protocol is cross–referenced to the appropriate pages in the original manual. This affordable companion volume, designed for bench use, offers individual investigators the opportunity to have their own personal collection of short protocols from the essential Molecular Cloning.
Author : Melody S. Clark
Publisher : Springer Science & Business Media
ISBN 13 : 3642878733
Total Pages : 551 pages
Book Rating : 4.6/5 (428 download)
Download or read book Plant Molecular Biology — A Laboratory Manual written by Melody S. Clark and published by Springer Science & Business Media. This book was released on 2013-11-27 with total page 551 pages. Available in PDF, EPUB and Kindle. Book excerpt: Covering the whole range of molecular biology techniques - genetic engineering as well as cytogenetics of plants -, each chapter begins with an introduction to the basic approach. followed by detailed methods with easy-to-follow protocols and comprehensive troubleshooting. The first part introduces basic molecular methodology such as DNA extraction, blotting, production of libraries and RNA cloning, while the second part describes analytical approaches, in particular RAPD and RFLP. The manual concludes with a variety of gene transfer techniques and both molecular and cytological analysis. As such, this will be of great use to both the first-timer and the experienced scientist.
Author : Stefan Surzycki
Publisher : John Wiley & Sons
ISBN 13 : 0470999241
Total Pages : 246 pages
Book Rating : 4.4/5 (79 download)
Download or read book Human Molecular Biology Laboratory Manual written by Stefan Surzycki and published by John Wiley & Sons. This book was released on 2008-04-15 with total page 246 pages. Available in PDF, EPUB and Kindle. Book excerpt: Human Molecular Biology Laboratory Manual offers a hands-on, state-of-the-art introduction to modern molecular biology techniques as applied to human genome analysis. In eight unique experiments, simple step-by-step instructions guide students through the basic principles of molecular biology and the latest laboratory techniques. This laboratory manual's distinctive focus on human molecular biology provides students with the opportunity to analyze and study their own genes while gaining real laboratory experience. A Background section highlighting the theoretical principles for each experiment. Safety Precautions. Technical Tips. Expected Results. Simple icons indicating tube orientation in centrifuge. Experiment Flow Charts Spiral bound for easy lab use
Author : Erica A. Golemis
Publisher : CSHL Press
ISBN 13 : 0879697237
Total Pages : 954 pages
Book Rating : 4.8/5 (796 download)
Download or read book Protein-protein Interactions written by Erica A. Golemis and published by CSHL Press. This book was released on 2005 with total page 954 pages. Available in PDF, EPUB and Kindle. Book excerpt: Reflecting the various advances in the field, this book provides comprehensive coverage of protein-protein interactions. It presents a collection of the technical and theoretical issues involved in the study of protein associations, including biophysical approaches. It also offers a collection of computational methods for analyzing interactions.
Author : K. V. CHAITANYA
Publisher : PHI Learning Pvt. Ltd.
ISBN 13 : 8120348001
Total Pages : 156 pages
Book Rating : 4.1/5 (23 download)
Download or read book CELL AND MOLECULAR BIOLOGY written by K. V. CHAITANYA and published by PHI Learning Pvt. Ltd.. This book was released on 2013-06-21 with total page 156 pages. Available in PDF, EPUB and Kindle. Book excerpt: This laboratory guide, intended for undergraduate and postgraduate students, includes techniques and their protocols ranging from microscopy to in vitro protein synthesis. Experiments relating to chromosomes study and identifying the phases of cell division are explained. The book lucidly deals with the extraction and characteri-zation of chromatin and techniques for studying its modifications, the gene methodology for identification of mutation and the methodology for isolation of nucleic acids from all types of organisms, such as viruses, fungi, plants and animals. All the protocols have been explained following step-by-step method. Different types of electrophoresis and their techniques, including blotting techniques and the methodology for stripping of probes from membranes for reusing the blot, have also been dealt with. Protocols on modern molecular biology techniques—PCR, restriction enzyme digest, DNA isolation, cloning and DNA sequencing—add weightage to the book. It also gives necessary knowledge of different types of stains, staining techniques, buffers, reagents and media used in the protocols. To help students prepare for answering viva voce questions, the book includes MCQs based on the discussed techniques.
Author : Khalid Z. Masoodi
Publisher : Academic Press
ISBN 13 : 012824450X
Total Pages : 220 pages
Book Rating : 4.1/5 (282 download)
Download or read book Advanced Methods in Molecular Biology and Biotechnology written by Khalid Z. Masoodi and published by Academic Press. This book was released on 2020-10-28 with total page 220 pages. Available in PDF, EPUB and Kindle. Book excerpt: Advanced Methods in Molecular Biology and Biotechnology: A Practical Lab Manual is a concise reference on common protocols and techniques for advanced molecular biology and biotechnology experimentation. Each chapter focuses on a different method, providing an overview before delving deeper into the procedure in a step-by-step approach. Techniques covered include genomic DNA extraction using cetyl trimethylammonium bromide (CTAB) and chloroform extraction, chromatographic techniques, ELISA, hybridization, gel electrophoresis, dot blot analysis and methods for studying polymerase chain reactions. Laboratory protocols and standard operating procedures for key equipment are also discussed, providing an instructive overview for lab work. This practical guide focuses on the latest advances and innovations in methods for molecular biology and biotechnology investigation, helping researchers and practitioners enhance and advance their own methodologies and take their work to the next level. - Explores a wide range of advanced methods that can be applied by researchers in molecular biology and biotechnology - Features clear, step-by-step instruction for applying the techniques covered - Offers an introduction to laboratory protocols and recommendations for best practice when conducting experimental work, including standard operating procedures for key equipment
Author : Edward Harlow
Publisher : CSHL Press
ISBN 13 : 9780879693145
Total Pages : 748 pages
Book Rating : 4.6/5 (931 download)
Download or read book Antibodies written by Edward Harlow and published by CSHL Press. This book was released on 1988 with total page 748 pages. Available in PDF, EPUB and Kindle. Book excerpt: Introduction to immunochemistry for molecular biologists and other nonspecialists. Spiral.
Author : Judith W. Zyskind
Publisher : Elsevier
ISBN 13 : 1483220974
Total Pages : 210 pages
Book Rating : 4.4/5 (832 download)
Download or read book Recombinant DNA Laboratory Manual written by Judith W. Zyskind and published by Elsevier. This book was released on 2014-05-12 with total page 210 pages. Available in PDF, EPUB and Kindle. Book excerpt: Recombinant DNA Laboratory Manual is a laboratory manual on the fundamentals of recombinant DNA techniques such as gel electrophoresis, in vivo mutagenesis, restriction mapping, and DNA sequencing. Procedures that are useful for studying either prokaryotes or eukaryotes are discussed, and experiments are included to teach the fundamentals of recombinant DNA technology. Hands-on computer sessions are also included to teach students how to enter and manipulate sequence information. Comprised of nine chapters, this book begins with an introduction to bacterial growth parameters, how to measure bacterial cell growth, and how to plot cell growth data. The discussion then turns to the isolation and analysis of chromosomal DNA in bacteria and Drosophila; plasmid DNA isolation and agarose gel analysis; and introduction of DNA into cells. Subsequent chapters deal with Tn5 mutagenesis of pBR329; DNA cloning in M13; DNA sequencing; and DNA gel blotting, probe preparation, hybridization, and hybrid detection. The book concludes with an analysis of lambda phage manipulations. This manual is intended for advanced undergraduate or beginning graduate students and should also be helpful to established investigators who are changing their research focus.
Author : S. K. Gakhar
Publisher :
ISBN 13 : 9789382332305
Total Pages : 0 pages
Book Rating : 4.3/5 (323 download)
Download or read book Molecular Biology written by S. K. Gakhar and published by . This book was released on 2013-09-30 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: The objective of this text is to train young teachers from colleges and research institutions so that they can advance their research in various fields of biology. It will also help students at BSc and MSc level to learn the techniques involved in molecular biology. The book contains four chapters providing step-by-step protocols. In addition, it has general instructions for safety procedures.
Author : Stefan Surzycki
Publisher : Springer Science & Business Media
ISBN 13 : 3642569684
Total Pages : 442 pages
Book Rating : 4.6/5 (425 download)
Download or read book Basic Techniques in Molecular Biology written by Stefan Surzycki and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 442 pages. Available in PDF, EPUB and Kindle. Book excerpt: This laboratory manual gives a thorough introduction to basic techniques. It is the result of practical experience, with each protocol having been used extensively in undergraduate courses or tested in the authors laboratory. In addition to detailed protocols and practical notes, each technique includes an overview of its general importance, the time and expense involved in its application and a description of the theoretical mechanisms of each step. This enables users to design their own modifications or to adapt the method to different systems. Surzycki has been holding undergraduate courses and workshops for many years, during which time he has extensively modified and refined the techniques described here.
Author : Carlos F. Barbas
Publisher : CSHL Press
ISBN 13 : 0879697407
Total Pages : 724 pages
Book Rating : 4.8/5 (796 download)
Download or read book Phage Display written by Carlos F. Barbas and published by CSHL Press. This book was released on 2001 with total page 724 pages. Available in PDF, EPUB and Kindle. Book excerpt: Phage-display technology has begun to make critical contributions to the study of molecular recognition. DNA sequences are cloned into phage, which then present on their surface the proteins encoded by the DNA. Individual phage are rescued through interaction of the displayed protein with a ligand, and the specific phage is amplified by infection of bacteria. Phage-display technology is powerful but challenging and the aim of this manual is to provide comprehensive instruction in its theoretical and applied so that any scientist with even modest molecular biology experience can effectively employ it. The manual reflects nearly a decade of experience with students of greatly varying technical expertise andexperience who attended a course on the technology at Cold Spring Harbor Laboratory. Phage-display technology is growing in importance and power. This manual is an unrivalled source of expertise in its execution and application.
Author : Josefine Liljeruhm
Publisher : World Scientific
ISBN 13 : 9814579564
Total Pages : 205 pages
Book Rating : 4.8/5 (145 download)
Download or read book Synthetic Biology: A Lab Manual written by Josefine Liljeruhm and published by World Scientific. This book was released on 2014-04-16 with total page 205 pages. Available in PDF, EPUB and Kindle. Book excerpt: Synthetic Biology: A Lab Manual is the first manual for laboratory work in the new and rapidly expanding field of synthetic biology. Aimed at non-specialists, it details protocols central to synthetic biology in both education and research. In addition, it provides all the information that teachers and students from high schools and tertiary institutions need for a colorful lab course in bacterial synthetic biology using chromoproteins and designer antisense RNAs. As a bonus, practical material is provided for students of the annual international Genetically Engineered Machine (iGEM) competition. The manual is based upon a highly successful course at Sweden's Uppsala University and is coauthored by one of the pioneers of synthetic biology and two bioengineering postgraduate students.An inspiring foreword is written by another pioneer in the field, Harvard's George Church: “Synthetic biology is to early recombinant DNA as a genome is to a gene. Is there anything that SynBio will not impact? There was no doubt that the field of SynBio needed ‘A Lab Manual’ such as the one that you now hold in your hands.”
Author : Zachary F. Burton
Publisher : Academic Press
ISBN 13 : 9780121473709
Total Pages : 252 pages
Book Rating : 4.4/5 (737 download)
Download or read book Experiments in Molecular Biology written by Zachary F. Burton and published by Academic Press. This book was released on 1997-02-20 with total page 252 pages. Available in PDF, EPUB and Kindle. Book excerpt: Experiments in Molecular Biology provides a thorough introduction to recombinant DNA methods used in molecular biology and nucleic acid biochemistry. This unique laboratory manual is particularly appropriate for courses in molecular cloning, molecular genetics techniques, molecular biology techniques, recombinant DNA techniques, bacterial genetics techniques, and genetic engineering. Included is an especially helpful section to aid new instructors in avoiding potential pitfalls of specific experiments. Key Features * Contains student-tested, easy-to-follow protocols * Presents background information that reinforces principles behind the methods presented * Includes questions at the end of laboratory exercises * Provides both detailed descriptions of experimental procedures and a theoretical support section * Sequentially links experiments to provide a "project" approach to studying molecular biochemistry * Includes student-tested, easy-to-follow protocols * Background information reinforces principles behind the methods presented * Includes questions at the end of laboratory exercises * Advises new instructors on potential pitfalls of specific experiments * Provides both detailed descriptions of experimental procedures and a theoretical support section * Sequentially links experiments to provide a "project" approach to studying
Author : J. Saxena
Publisher : Scientific Publishers
ISBN 13 : 9386237237
Total Pages : 402 pages
Book Rating : 4.3/5 (862 download)
Download or read book Laboratory Manual of Microbiology, Biochemistry and Molecular Biology written by J. Saxena and published by Scientific Publishers. This book was released on 2015-05-01 with total page 402 pages. Available in PDF, EPUB and Kindle. Book excerpt: Though many practical books are available in the market but this Laboratory Manual of Microbiology, Biochemistry and Molecular Biology is an unique combination of protocols that covers maximum (about 80%) of the practicals of various Indian universities for UG and PG courses in Bioscience, Biotechnology, Microbiology, Biochemistry and Biochemical Engineering.
Author : R. M. Maizels
Publisher : CUP Archive
ISBN 13 : 9780521419277
Total Pages : 246 pages
Book Rating : 4.4/5 (192 download)
Download or read book Parasite Antigens, Parasite Genes written by R. M. Maizels and published by CUP Archive. This book was released on 1991 with total page 246 pages. Available in PDF, EPUB and Kindle. Book excerpt: Enzymes; 8.
