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Using Mass Spectrometry To Investigate Protein Surface Interactions During Hydrophobic Interaction Chromatography
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Book Synopsis Using Mass Spectrometry to Investigate Protein-surface Interactions During Hydrophobic Interaction Chromatography by : Tara Tibbs Jones
Download or read book Using Mass Spectrometry to Investigate Protein-surface Interactions During Hydrophobic Interaction Chromatography written by Tara Tibbs Jones and published by . This book was released on 2003 with total page 310 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Protein-surface Interactions in Chromatography by : Tracy Wilson Perkins
Download or read book Protein-surface Interactions in Chromatography written by Tracy Wilson Perkins and published by . This book was released on 1997 with total page 440 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Mass Spectrometry Analysis for Protein-Protein Interactions and Dynamics by : M. Chance
Download or read book Mass Spectrometry Analysis for Protein-Protein Interactions and Dynamics written by M. Chance and published by John Wiley & Sons. This book was released on 2008-09-22 with total page 325 pages. Available in PDF, EPUB and Kindle. Book excerpt: Presents a wide variety of mass spectrometry methods used to explore structural mechanisms, protein dynamics and interactions between proteins. Preliminary chapters cover mass spectrometry methods for examining proteins and are then followed by chapters devoted to presenting very practical, how-to methods in a detailed way. Includes footprinting and plistex specifically, setting this book apart from the competition.
Book Synopsis Protein Retention and Transport in Hydrophobic Interaction Chromatography by :
Download or read book Protein Retention and Transport in Hydrophobic Interaction Chromatography written by and published by . This book was released on 2006 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: Hydrophobic interaction chromatography (HIC) is gaining popularity for purification of biologics because it accomplishes separation based on a different set of molecular characteristics to more popular methods such as ion-exchange chromatography. The existing retention models for HIC account for the effect of salt, but the effects of adsorbent and protein properties are not completely understood. In addition, very few studies have been performed to study protein transport in HIC although this area is important to process design and scale-up. The goals of this thesis were to characterize the adsorbents used in HIC and to determine adsorption and transport properties necessary for designing HIC processes. First, inverse size exclusion chromatography was used to determine the key physical properties, specifically the pore size distributions, of a set of HIC adsorbents. These were then used to explain subsequently measured adsorption and transport trends. Isocratic elution experiments showed a very clear trend in that protein molecular mass and structural stability affect retention, while protein recovery is sensitive to structural stability and especially the nature of the adsorbent base matrix. A general thermodynamic relation was derived that predicts that protein retention in HIC increases under conditions that decrease protein solubility. This correlation is consistent with the experimental results that solubility, in terms of its surrogate the second osmotic virial coefficient, correlates well with HIC retention in many cases, including correctly predicting the reverse Hofmeister effects. However, solubility could not explain retention behavior under some conditions. In those cases, protein-surface interactions or conformational change could be important determinants of protein adsorption. The adsorption isotherms of proteins in HIC were relatively "soft", and well-defined plateau regions were not observed. The static capacities of the media varied depending on salt concentration and the protein and adsorbent types. The protein accessible surface area appears to be the main factor determining the binding capacity. The dynamic capacities of the adsorbents were 10--70% of the static capacities, depending on adsorbent particle size and feed flow rate. The effective pore diffusivities of model proteins in HIC media were determined using the general linear rate model. The pore diffusivities obtained from this method are generally accurate for proteins with low structural flexibility but not for more flexible ones, presumably because conformational change effects contribute significantly to the overall rate limitations. (Abstract shortened by UMI.).
Book Synopsis Controlling Protein-surface Interactions in Chromatography Using Mixed Self-assembled Monolayers by : Chrysanty Tedjo
Download or read book Controlling Protein-surface Interactions in Chromatography Using Mixed Self-assembled Monolayers written by Chrysanty Tedjo and published by . This book was released on 2011 with total page 157 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Guide to Protein Purification by : Richard R Burgess
Download or read book Guide to Protein Purification written by Richard R Burgess and published by Academic Press. This book was released on 2009-11-03 with total page 915 pages. Available in PDF, EPUB and Kindle. Book excerpt: Guide to Protein Purification, Second Edition provides a complete update to existing methods in the field, reflecting the enormous advances made in the last two decades. In particular, proteomics, mass spectrometry, and DNA technology have revolutionized the field since the first edition’s publication but through all of the advancements, the purification of proteins is still an indispensable first step in understanding their function. This volume examines the most reliable, robust methods for researchers in biochemistry, molecular and cell biology, genetics, pharmacology and biotechnology and sets a standard for best practices in the field. It relates how these traditional and new cutting-edge methods connect to the explosive advancements in the field. This "Guide to" gives imminently practical advice to avoid costly mistakes in choosing a method and brings in perspective from the premier researchers while presents a comprehensive overview of the field today. Gathers top global authors from industry, medicine, and research fields across a wide variety of disciplines, including biochemistry, genetics, oncology, pharmacology, dermatology and immunology Assembles chapters on both common and less common relevant techniques Provides robust methods as well as an analysis of the advancements in the field that, for an individual investigator, can be a demanding and time-consuming process
Book Synopsis Ion-Exchange Chromatography of Proteins by : Shuichi Yamamoto
Download or read book Ion-Exchange Chromatography of Proteins written by Shuichi Yamamoto and published by CRC Press. This book was released on 1988-04-14 with total page 438 pages. Available in PDF, EPUB and Kindle. Book excerpt: Showing chemists how to predict ion-exchange chromatography (IEC) separation behavior and how to determine appropriate operating conditions, this reference illustrates procedures, apparatus, and types of ion exchangers, emphasizing the design and application of large-scale IEC. Complete with more than 100 useful tables and diagrams, Ion-Exchange Chromatography of Proteins explains the effects of each variable on separation behavior ... compares models and equations describing separation behavior of proteins in IEC with experimental results ... introduces design calculation procedures for scale up, adapting IEC for individual requirements ... discusses parameters affecting separation behavior, including adsorption equilibria, stationary phase diffusion, and axial dispersion ... provides examples of the most up-to-date applications, such as high- or medium-performance IEC and large-scale operations ... and reviews both theoretical and experimental literature. Ion-Exchange Chromatography of Proteins serves as an important reference for analytical, agricultural, food, and pharmaceutical chemists, chromatographers, food scientists and technologists, biochemists, and biotechnologists. Book jacket.
Book Synopsis Surface Energetics of Protein Adsorption Onto Chromatographic Supports by : Muhammad Aasim
Download or read book Surface Energetics of Protein Adsorption Onto Chromatographic Supports written by Muhammad Aasim and published by . This book was released on 2012 with total page 257 pages. Available in PDF, EPUB and Kindle. Book excerpt: Protein adsorption onto chromatographic supports has been studied utilizing the extended DLVO approach. Hydrophobic interaction chromatography was mainly explored due to it wide applicability in the recovery of a number of biologically significant macromolecules in its native form. The interactions between a number of selected model proteins and commercially available chromatographic beads i.e., Phenyl Sepharose 6 Fast Flow, Source 15 Phenyl, Toyopearl Phenyl 650-C and Toyopearl Butyl 650-C was studied via extended DLVO (XDLVO) calculations. The physicochemical properties of the proteins and the chromatographic beads, required for the calculations, are achieved by contact angle measurements and zeta potential determinations. Protein properties were investigated both at the hydrated and dehydrated state to achieve the colloidal-type calculation at the low and high salts. The mentioned approach allowed the calculations of interaction energy of proteins to chromatographic supports as a function of distance, at the operating buffer conditions. The XDLVO calculations were correlated with the actual separation behavior of the model proteins. The correlations plotted for all the chromatographic supports under study revealed that all the proteins can be segregated into two main groups; the proteins showing higher interaction energy minimum are eluting late in the chromatography while proteins showing low interaction energy are eluting earlier in the chromatographic experiments. Moreover, the calculations were able to expose the role of backbone chemistry harboring the same ligand and the effect of different ligands immobilized on the same supports. These studies have also shown the effect of the supporting surface used for protein immobilization. Thus, the XDLVO calculations were able to understand the underlying phenomenon and will suggest a broader tool to enhance a better understanding of the downstream bioprocessing, which will facilitate development, optimization and finally implementation of the bioprocess design.
Book Synopsis Mass Spectrometry of Protein Interactions by : Kevin Downard
Download or read book Mass Spectrometry of Protein Interactions written by Kevin Downard and published by John Wiley & Sons. This book was released on 2007-08-24 with total page 153 pages. Available in PDF, EPUB and Kindle. Book excerpt: The authoritative guide to analyzing protein interactions by mass spectrometry Mass spectrometry (MS) is playing an increasingly important role in the study of protein interactions. Mass Spectrometry of Protein Interactionspresents timely and definitive discussions of the diverse range of approaches for studying protein interactions by mass spectrometry with an extensive set of references to the primary literature. Each chapter is written by authors or teams of authors who are international authorities in their fields. This leading reference text: * Discusses the direct detection of protein interactions through electrospray ionization (ESI-MS); ion mobility analysis; and matrix-assisted laser desorption/ionization (MALDI-MS) * Covers the indirect analysis of protein interactions through hydrogen-deuterium exchange (HX-MS); limited proteolysis; cross-linking; and radial probe (RP-MS) * Guides researchers in the use of mass spectrometry in structural biology, biochemistry, and protein science to map and define the huge number and diversity of protein interactions * Reviews the latest discoveries and applications and addresses new and ongoing challenges This is a comprehensive reference for researchers in academia and industry engaged in studies of protein interactions and an excellent text for graduate and postgraduate students.
Book Synopsis Purification Tools for Monoclonal Antibodies by : Pete Gagnon
Download or read book Purification Tools for Monoclonal Antibodies written by Pete Gagnon and published by Validated Biosystems Incorporated. This book was released on 1996 with total page 278 pages. Available in PDF, EPUB and Kindle. Book excerpt: Purification Tools for Monoclonal is an essential book for professionals, educators and advanced students in the field of Biotechnology. It is based on experience gained from purification process development, scale-up, and manufacture of more than 250 monoclonal-based diagnostic and therapeutic products. Ten chapters provide in-depth coverage of major separation mechanisms, process strengths, weaknesses and method development; all fully integrated with the special performance, economic and validation requirements associated with monoclonals. Covered methods include precipitation with inorganic salts, polyethylene glycol, electrolyte depletion, caprylic acid, ethacridine, chromatographic purification by size exclusion, ion exchange, hydroxyapatite, hydrophobic interaction, immobilized metal affinity, hydrophilic interaction, euglobulin adsorption, thiophilic adsorption, protein A, protein G, lectin affinity, and more. 88 figures, 29 tables.
Book Synopsis Proteomic Profiling and Analytical Chemistry by : Pawel Ciborowski
Download or read book Proteomic Profiling and Analytical Chemistry written by Pawel Ciborowski and published by Elsevier. This book was released on 2016-03-02 with total page 300 pages. Available in PDF, EPUB and Kindle. Book excerpt: Proteomic Profiling and Analytical Chemistry: The Crossroads, Second Edition helps scientists without a strong background in analytical chemistry to understand principles of the multistep proteomic experiment necessary for its successful completion. It also helps researchers who do have an analytical chemistry background to break into the proteomics field. Highlighting points of junction between proteomics and analytical chemistry, this resource links experimental design with analytical measurements, data analysis, and quality control. This targeted point of view will help both biologists and chemists to better understand all components of a complex proteomic study. The book provides detailed coverage of experimental aspects such as sample preparation, protein extraction and precipitation, gel electrophoresis, microarrays, dynamics of fluorescent dyes, and more. The key feature of this book is a direct link between multistep proteomic strategy and quality control routinely applied in analytical chemistry. This second edition features a new chapter on SWATH-MS, substantial updates to all chapters, including proteomic database search and analytical quantification, expanded discussion of post-hoc statistical tests, and additional content on validation in proteomics. Covers the analytical consequences of protein and peptide modifications that may have a profound effect on how and what researchers actually measure Includes practical examples illustrating the importance of problems in quantitation and validation of biomarkers Helps in designing and executing proteomic experiments with sound analytics
Book Synopsis Biological Interactions on Materials Surfaces by : David A. Puleo
Download or read book Biological Interactions on Materials Surfaces written by David A. Puleo and published by Springer Science & Business Media. This book was released on 2009-06-26 with total page 438 pages. Available in PDF, EPUB and Kindle. Book excerpt: Success or failure of biomaterials, whether tissue engineered constructs, joint and dental implants, vascular grafts, or heart valves, depends on molecular-level events that determine subsequent responses of cells and tissues. This book presents the latest developments and state-of-the-art knowledge regarding protein, cell, and tissue interactions with both conventional and nanophase materials. Insight into these biomaterial surface interactions will play a critical role in further developments in fields such as tissue engineering, regenerative medicine, and biocompatibility of implanted materials and devices. With chapters written by leaders in their respective fields, this compendium will be the authoritative source of information for scientists, engineers, and medical researchers seeking not only to understand but also to control tissue-biomaterial interactions.
Book Synopsis Mass Spectrometry for Microbial Proteomics by : Haroun N. Shah
Download or read book Mass Spectrometry for Microbial Proteomics written by Haroun N. Shah and published by John Wiley & Sons. This book was released on 2010-10-28 with total page 545 pages. Available in PDF, EPUB and Kindle. Book excerpt: New advances in proteomics, driven largely by developments in mass spectrometry, continue to reveal the complexity and diversity of pathogenic mechanisms among microbes that underpin infectious diseases. Therefore a new era in medical microbiology is demanding a rapid transition from current procedures to high throughput analytical systems for the diagnosis of microbial pathogens. This book covers the broad microbiological applications of proteomics and mass spectrometry. It is divided into six sections that follow the general progression in which most microbiology laboratories are approaching the subject –Transition, Tools, Preparation, Profiling by Patterns, Target Proteins, and Data Analysis.
Book Synopsis Protein Purification by : Jan-Christer Janson
Download or read book Protein Purification written by Jan-Christer Janson and published by John Wiley & Sons. This book was released on 1998 with total page 712 pages. Available in PDF, EPUB and Kindle. Book excerpt: This is a state-of-the-art sourcebook on modern high-resolution biochemical separation techniques for proteins. It contains all the basic theory and principles used in protein chromatography and electrophoresis.
Book Synopsis Protein-surface Interactions in Multimodal Chromatography: a Molecular Modeling Based Investigation by : Suvrajit Banerjee
Download or read book Protein-surface Interactions in Multimodal Chromatography: a Molecular Modeling Based Investigation written by Suvrajit Banerjee and published by . This book was released on 2016 with total page 258 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Proteomics Sample Preparation by : Jörg von Hagen
Download or read book Proteomics Sample Preparation written by Jörg von Hagen and published by John Wiley & Sons. This book was released on 2011-08-24 with total page 498 pages. Available in PDF, EPUB and Kindle. Book excerpt: This long-awaited first guide to sample preparation for proteomics studies overcomes a major bottleneck in this fast growing technique within the molecular life sciences. By addressing the topic from three different angles -- sample, method and aim of the study -- this practical reference has something for every proteomics researcher. Following an introduction to the field, the book looks at sample preparation for specific techniques and applications and finishes with a section on the preparation of sample types. For each method described, a summary of the pros and cons is given, as well as step-by-step protocols adaptable to any specific proteome analysis task.
Book Synopsis Mass Spectrometry-Based Chemical Proteomics by : W. Andy Tao
Download or read book Mass Spectrometry-Based Chemical Proteomics written by W. Andy Tao and published by John Wiley & Sons. This book was released on 2019-07-10 with total page 448 pages. Available in PDF, EPUB and Kindle. Book excerpt: PROVIDES STRATEGIES AND CONCEPTS FOR UNDERSTANDING CHEMICAL PROTEOMICS, AND ANALYZING PROTEIN FUNCTIONS, MODIFICATIONS, AND INTERACTIONS—EMPHASIZING MASS SPECTROMETRY THROUGHOUT Covering mass spectrometry for chemical proteomics, this book helps readers understand analytical strategies behind protein functions, their modifications and interactions, and applications in drug discovery. It provides a basic overview and presents concepts in chemical proteomics through three angles: Strategies, Technical Advances, and Applications. Chapters cover those many technical advances and applications in drug discovery, from target identification to validation and potential treatments. The first section of Mass Spectrometry-Based Chemical Proteomics starts by reviewing basic methods and recent advances in mass spectrometry for proteomics, including shotgun proteomics, quantitative proteomics, and data analyses. The next section covers a variety of techniques and strategies coupling chemical probes to MS-based proteomics to provide functional insights into the proteome. In the last section, it focuses on using chemical strategies to study protein post-translational modifications and high-order structures. Summarizes chemical proteomics, up-to-date concepts, analysis, and target validation Covers fundamentals and strategies, including the profiling of enzyme activities and protein-drug interactions Explains technical advances in the field and describes on shotgun proteomics, quantitative proteomics, and corresponding methods of software and database usage for proteomics Includes a wide variety of applications in drug discovery, from kinase inhibitors and intracellular drug targets to the chemoproteomics analysis of natural products Addresses an important tool in small molecule drug discovery, appealing to both academia and the pharmaceutical industry Mass Spectrometry-Based Chemical Proteomics is an excellent source of information for readers in both academia and industry in a variety of fields, including pharmaceutical sciences, drug discovery, molecular biology, bioinformatics, and analytical sciences.
