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Author : Zandiswa Modjadji Salman
Publisher :
ISBN 13 :
Total Pages : 202 pages
Book Rating : 4.:/5 (775 download)
Download or read book Transformation of Cassava (Manihot Esculenta Crantz). written by Zandiswa Modjadji Salman and published by . This book was released on 2000 with total page 202 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author : V. N. H. van Velzen
Publisher :
ISBN 13 :
Total Pages : 154 pages
Book Rating : 4.:/5 (671 download)
Download or read book Optimization of Transformation and Regeneration in Cassava (Manihot Esculenta Crantz) written by V. N. H. van Velzen and published by . This book was released on 2000 with total page 154 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author : Hang Tu Luong
Publisher :
ISBN 13 :
Total Pages : pages
Book Rating : 4.:/5 (595 download)
Download or read book Studies on Transformation of Cassava (Manihot Esculenta Crantz.). written by Hang Tu Luong and published by . This book was released on 1996 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author : Peng Zhang
Publisher :
ISBN 13 :
Total Pages : 258 pages
Book Rating : 4.:/5 (636 download)
Download or read book Studies on Cassava (Manihot Esculenta Crantz) Transformation: Towards Genetic Improvement written by Peng Zhang and published by . This book was released on 2000 with total page 258 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author : Ian S. Curtis
Publisher : Springer Science & Business Media
ISBN 13 : 1402023332
Total Pages : 448 pages
Book Rating : 4.4/5 (2 download)
Download or read book Transgenic Crops of the World written by Ian S. Curtis and published by Springer Science & Business Media. This book was released on 2012-08-10 with total page 448 pages. Available in PDF, EPUB and Kindle. Book excerpt: Since the first transgenic plants were produced back in the early 1980s, there have been substantial developments towards the genetic engineering of most crops of our world. Initial studies using isolated plant cells and removing their cell walls to form protoplasts, offered the possibility of transferring genetic material by Agrobacterium-mediated gene transfer, chemical agents or electrical charges. However, in those cases were isolated protoplasts could be transformed, often, a shoot regeneration system was not available to induce the production of transgenic plants and any such regenerated plants were subject to mutation or chromosomal of cultured plant organs, such as leaf abnormalities. By the mid-1980s, the use disks, offered the convenience of combining gene transfer, plant regeneration and selection of transformants in a single system. This approach, enabled the production of stable, phenotypically-normal, transgenic potato and tomato plants in culture. By the late 1980s, the use of biolistics offered a means of inserting foreign genes into plant cells which where inaccessible to Agrobacterium infection. Even today, this technology is now standard practice for the production of some transgenic plants.
Author : Tiyani Yvonne Mathebula
Publisher :
ISBN 13 :
Total Pages : 208 pages
Book Rating : 4.:/5 (775 download)
Download or read book Transformation of South African Cassava (Manihot Esculenta Crantz) Cultivar written by Tiyani Yvonne Mathebula and published by . This book was released on 2001 with total page 208 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author : Eri Sofiari
Publisher :
ISBN 13 : 9789054855361
Total Pages : 136 pages
Book Rating : 4.8/5 (553 download)
Download or read book Regeneration and Transformation of Cassava (Manihot Esculenta Crantz.) written by Eri Sofiari and published by . This book was released on 1996 with total page 136 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author : Uzoma Enyinnaya Ihemere
Publisher :
ISBN 13 :
Total Pages : pages
Book Rating : 4.:/5 (565 download)
Download or read book Somatic Embryogenesis and Transformation of Cassava for Enhanced Starch Production written by Uzoma Enyinnaya Ihemere and published by . This book was released on 2003 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: Abstract: Cassava (Manihot esculenta Crantz), a member of the family Euphorbiaceae, is one of the most important food crops of sub-Saharan Africa. One of the constraints for cassava starch production is the long growing season. Cassava typically takes 9-12 months to yield a good harvest. This is longer than other major starch-producing crops such as corn and potatoes. We report here the generation of transgenic cassava with increased starch biosynthesis capacity. This was achieved by enhancing the activity of ADP-glucose pyrophosphorylase (AGPase), the rate-limiting enzyme in the starch biosynthesis, in transgenic cassava. To do this, we transformed cassava with a modified E. coli glgC gene encoding AGPase. The glgC gene was modified by site-directed mutagenesis (K296E/G336D) to remove the allosteric regulation (enhancement by fructose1,6-P and inhibition by AMP) sites and to increase the velocity of the enzyme. Root-specific expression of the glgC gene product was achieved using the tuber-specific patatin promoter of potato. We obtained antibiotic-resistant putative transformed plants which have been shown to have integrated and expressed the transgene by PCR, Southern blot, RT-PCR and enzyme activity analyses. AGPase enzyme activity in transformed plants was increased by more than 65%. Significantly, transgenic plants expressing the bacterial glgC gene had two-fold greater top (leaf and stem) and root biomass than wild-type plants grown in the greenhouse.
Author : Edouard Pichko Kassianoff
Publisher :
ISBN 13 :
Total Pages : 624 pages
Book Rating : 4.:/5 (86 download)
Download or read book Biomas Partitioning Characteristics, Tissue Culture and Transformation of the Genetic Improvement of Cassava (manihot Esculenta Crantz) written by Edouard Pichko Kassianoff and published by . This book was released on 1992 with total page 624 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author : Sandra Laminski
Publisher :
ISBN 13 :
Total Pages : 404 pages
Book Rating : 4.:/5 (775 download)
Download or read book Molecular Characterisation and Transformation of South African Cassava (Manihot Esculenta Crantz). written by Sandra Laminski and published by . This book was released on 2003 with total page 404 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author : Murunwa Makwarela
Publisher :
ISBN 13 :
Total Pages : 360 pages
Book Rating : 4.:/5 (775 download)
Download or read book Optimisation of Regeneration Systems for a Range of Cassava (Manihot Esculenta Crantz) Cultivars Suitable for Growth in South Africa and Transformation with SACMV N-Rep Gene written by Murunwa Makwarela and published by . This book was released on 2006 with total page 360 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author : M. M. Schreuder
Publisher :
ISBN 13 :
Total Pages : 36 pages
Book Rating : 4.:/5 (672 download)
Download or read book Transformation and Regeneration of Cassava (Manihot Esculanta Crantz.) written by M. M. Schreuder and published by . This book was released on 1998 with total page 36 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author : Viduranga Waisundara
Publisher : BoD – Books on Demand
ISBN 13 : 9535137409
Total Pages : 318 pages
Book Rating : 4.5/5 (351 download)
Download or read book Cassava written by Viduranga Waisundara and published by BoD – Books on Demand. This book was released on 2018-01-17 with total page 318 pages. Available in PDF, EPUB and Kindle. Book excerpt: Cassava is a staple food for many nations owing to its resilience for growth under various climatic conditions. It is a good source of carbohydrates and is the third largest source of food carbohydrates in the tropics, after rice and maize. This book focuses on the morphological traits and nutritive properties of cassava and its production processes, postharvest techniques and diseases that affect the growth of the crop. Given its extensive usage and market value, it is one of the agricultural produces for which many biotechnological interventions have been applied for ascertaining food security. It is hoped that readers will gain knowledge on cassava as well as use some of the techniques mentioned herein for improvement of the production of the crop.
Author :
Publisher : Bib. Orton IICA / CATIE
ISBN 13 :
Total Pages : 50 pages
Book Rating : 4./5 ( download)
Download or read book Mutation Breeding in Cassava written by and published by Bib. Orton IICA / CATIE. This book was released on with total page 50 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author : Elizabeth Balyejusa Kizito
Publisher :
ISBN 13 : 9789157671318
Total Pages : 47 pages
Book Rating : 4.6/5 (713 download)
Download or read book Genetic and Root Growth Studies in Cassava (Manihot Esculenta Crantz) written by Elizabeth Balyejusa Kizito and published by . This book was released on 2006 with total page 47 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author : Felix I. Nweke
Publisher : MSU Press
ISBN 13 :
Total Pages : 322 pages
Book Rating : 4.F/5 ( download)
Download or read book The Cassava Transformation written by Felix I. Nweke and published by MSU Press. This book was released on 2002 with total page 322 pages. Available in PDF, EPUB and Kindle. Book excerpt: Cassava is Africa's second most important food crop. The cassava transformation that is now underway in West Africa is fueled by new high yielding TMS varieties that have transformed cassava from a low-yielding, famine-reserve crop to a high-yielding cash crop for both rural and urban consumers. The book highlights the role of cassava as a "poverty fighter" by increasing cassava productivity and driving down the cost of cassava in rural and urban diets.
Author : Dimuth S. K. Siritunga
Publisher :
ISBN 13 :
Total Pages : pages
Book Rating : 4.:/5 (516 download)
Download or read book Generation of Acyanogenic Cassava (Manihot Esculenta Crants) written by Dimuth S. K. Siritunga and published by . This book was released on 2002 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: Abstract: Cassava (Manihot esculenta, Crantz) roots are the primary source of calories for more than 500 million people. Cassava leaves and roots, however, contain potentially toxic levels of the cyanogenic glycoside, linamarin. Consumption of residual cyanogens in incompletely processed cassava roots can result in cyanide poisoning due to conversion of the cyanogens to cyanide in the body. Our objective was to eliminate cyanogens from cassava so as to eliminate the need for extensive food processing. To achieve this goal we generated transgenic cassava plants in which the expression of the CYP79D1 and CYP79D2 genes, that catalyze the first-dedicated step in linamarin synthesis, was inhibited. Using a leaf-specific Cab1 promoter to drive the antisense expression of the CYP79D1/CYP79D2 genes we observed up to a 94% reduction in leaf linamarin content associated with an apparent complete inhibition of CYP79D1/CYP79D2 expression. Significantly, the linamarin content of roots also was reduced by 99% in transgenic plants. These results suggest that linamarin is transported from leaves to roots. To investigate linamarin transport further we generated transgenic cassava plants in which the expression of CYP79D1/CYP79D2 was inhibited using a tuber-specific patatin promoter to drive the antisense expression of the genes. Transformants with complete inhibition of CYP79D1/CYP79D2 expression in the roots had no reduction in the root linamarin content compared to wild-type plants. Thus it is apparent that young cassava plants (3-4 month old) transport cyanogenic glucosides from the leaves to the roots. It has been demonstrated that the major cyanogen present in poorly processed cassava roots is acetone cyanohydrin and that cassava roots have substantially lower levels of HNL than leaves. We postulated that elevated expression of HNL in roots would accelerate the conversion of acetone cyanohydrin to cyanide. To test this hypothesis we have over-expressed HNL in transgenic cassava plants under the control of 2X35S CaMV promoter. The 2-fold elevation of HNL levels was correlated with substantially reduced levels of acetone cyanohydrin in homogenized root tissues. Importantly the over-expression of HNL in roots of transgenic cassava plants does not affect the steady-state linamarin levels in intact roots, thus retaining the herbivore deterrent attributes of cyanogens.
