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Download or read book Trace Analysis of Peptides and Proteins by Capillary Electrophoresis written by and published by . This book was released on 1998 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
![Download Trace Analysis of Peptides and Proteins by Capillary Electrophoresis [microform] PDF Online Free](https://books.google.com/books/content/images/frontcover/YIG1SgAACAAJ?fife=w200-h370)
Author : Pinto, Devanand Michael
Publisher : National Library of Canada = Bibliothèque nationale du Canada
ISBN 13 :
Total Pages : 342 pages
Book Rating : 4.:/5 (74 download)
Download or read book Trace Analysis of Peptides and Proteins by Capillary Electrophoresis [microform] written by Pinto, Devanand Michael and published by National Library of Canada = Bibliothèque nationale du Canada. This book was released on 1998 with total page 342 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author : Mark A. Strege
Publisher : Springer Science & Business Media
ISBN 13 : 159259798X
Total Pages : 340 pages
Book Rating : 4.5/5 (925 download)
Download or read book Capillary Electrophoresis of Proteins and Peptides written by Mark A. Strege and published by Springer Science & Business Media. This book was released on 2008-02-04 with total page 340 pages. Available in PDF, EPUB and Kindle. Book excerpt: Throughout the more than 20 years that have followed the beginnings of capillary electrophoresis (CE), its application to the analysis of proteins and peptides has continued to be reliable, versatile, and productive. Over time, CE has matured to become a superb complement to HLPC, and in many cases has also evolved as an automated and quantitative replacement for conventional slab gel electrophoresis methods such as SDS-PAGE and isoelectric focusing. Within Capillary Electrophoresis of Proteins and Peptides, we have assembled contributions from researchers who are applying state-of-the-art CE for protein and peptide analysis, including topics that we believe are of great potential both in the present and for the future. In comparison to traditional separation methods, CE represents a miniaturized analysis technique (especially in its microchip-based format) that is highly dependent upon the basic fundamentals of effective sample recovery and high sensitivity detection. With these issues in mind, Chapters 1–4 describe recently developed approaches for both capillary coatings and analyte detection via laser-induced fluorescence. Since the discipline of biotechnology has established itself as a primary platform for the application of CE to the analysis of proteins and peptides, Chapters 5–7 demonstrate a variety of examples of the specific techniques that have been applied for the development of biopharmaceuticals and their commercialization. The methods covered here include also the analysis of oligosaccharides from glycoproteins.
Author : Nguyet Thuy Tran
Publisher : Humana
ISBN 13 : 9781493981533
Total Pages : 234 pages
Book Rating : 4.9/5 (815 download)
Download or read book Capillary Electrophoresis of Proteins and Peptides written by Nguyet Thuy Tran and published by Humana. This book was released on 2018-05-30 with total page 234 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book provides a comprehensive survey of recent developments and applications of high performance capillary electrophoresis in the field of protein and peptide analysis with a distinct focus on the analysis of intact proteins. With practical detail, the contents cover different modes of capillary electrophoresis (CE) useful for protein and peptide analysis, CZE, CIEF, ACE, CGE, and different types of application such as the quality control of therapeutic proteins and monoclonal antibodies, clinical analyses of chemokines in tissues, qualitative and quantitative analysis of vaccine proteins, and determination of binding constants in complexes involving peptides or proteins. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and exhaustive, Capillary Electrophoresis of Proteins and Peptides: Methods and Protocols serves both beginners and experts with a collection of the current and most active topics in this vital field of study.
Author : Angela R. Piergiovanni
Publisher : MDPI
ISBN 13 : 3039212273
Total Pages : 110 pages
Book Rating : 4.0/5 (392 download)
Download or read book Analysis of Peptides and Proteins by Electrophoretic Techniques written by Angela R. Piergiovanni and published by MDPI. This book was released on 2019-07-23 with total page 110 pages. Available in PDF, EPUB and Kindle. Book excerpt: The characterization of peptides and proteins is central to understanding their function and expression in biological matrices. Moreover, these macromolecules are important biomarkers of many human diseases. In recent years, the performance of separation techniques based on electromigration have significantly increased. The development of microdevices has reduced sample consumption and waste production while high-sensitivity detectors, such as mass spectrometry (MS) or laser-induced fluorescence (LIF), have significantly improved with regards to separation efficiency and detection limits. All of these advancements have led to appreciably enlarged fields of application. Nowadays, a multitude of studies using separation techniques based on electromigration to study proteins and peptides from numerous real matrices are available in the literature. This Special Issue covers the most recent knowledge and advances in the study of peptides and proteins using several electrophoresis techniques, as well as the characterization of relevant proteins and peptides in application areas such as clinical studies, functional foods, and toxicology.
Author : Tim Wehr
Publisher : CRC Press
ISBN 13 : 1482289857
Total Pages : 301 pages
Book Rating : 4.4/5 (822 download)
Download or read book Capillary Electrophoresis of Proteins written by Tim Wehr and published by CRC Press. This book was released on 1998-10-20 with total page 301 pages. Available in PDF, EPUB and Kindle. Book excerpt: "Provides practical information on the application of capillary electrophoresis (CE) to protein analysis, with an emphasis on developing and optimizing CE techniques in the laboratory. Includes separation methods bases on mass, charge, isoelectric point, molecular sieving, and affinity interactions."
Author : William S. Hancock
Publisher : CRC Press
ISBN 13 : 9780849378225
Total Pages : 280 pages
Book Rating : 4.3/5 (782 download)
Download or read book New Methods in Peptide Mapping for the Characterization of Proteins written by William S. Hancock and published by CRC Press. This book was released on 1995-10-23 with total page 280 pages. Available in PDF, EPUB and Kindle. Book excerpt: This text is devoted to the characterization of recombinant DNA-derived proteins by peptide mapping. It describes new technological procedures including capillary electrophoresis, analysis of glycopeptides and the use of electrospray and matrix-assisted laser desorption mass spectrometry. The book presents practical procedures for preparing a protein sample, the enzyme digestion, choice of separation method and procedures for the structural analysis of the separated species. Many figures of peptide maps illustrate typical results. Tables of summary information about digestion, separation conditions, and analyses of important protein samples are also presented.
Author : James Q. Xia
Publisher : Springer
ISBN 13 : 3319462407
Total Pages : 80 pages
Book Rating : 4.3/5 (194 download)
Download or read book Capillary Electrophoresis-Mass Spectrometry written by James Q. Xia and published by Springer. This book was released on 2016-11-08 with total page 80 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book covers the latest developments in capillary electrophoresis-mass spectrometry for the analysis of therapeutic proteins. The application of capillary electrophoresis-mass spectrometry (CE-MS) coupling technology in the analysis of recombinant therapeutic proteins is detailed thoroughly. Specific topics include recent developments in coupling capillary electrophoresis with mass spectrometry for the quality control of monoclonal antibody therapeutics, top-down analysis of monoclonal antibody using the CE-MS platform, and detection of host cell protein impurities. Comprehensive characterization of antibody-drug conjugates (ADCs) by coupling capillary electrophoresis with mass spectrometry is also covered. This is an ideal book for scientists in the life science and biopharmaceutical industry who are working on characterizing the PTMs of monoclonal antibodies, as well as graduate students and researchers in the separation science and biological mass spectrometry fields.
Author : Angelina Taichrib
Publisher :
ISBN 13 :
Total Pages : 115 pages
Book Rating : 4.:/5 (828 download)
Download or read book Analysis of Peptides and Proteins by Capillary Electrophoresis-mass Spectrometry written by Angelina Taichrib and published by . This book was released on 2012 with total page 115 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author : Lin Liu
Publisher :
ISBN 13 :
Total Pages : 242 pages
Book Rating : 4.3/5 (91 download)
Download or read book Development of Capillary Electrophoresis and Mass Spectrometry Methods in Protein Analysis written by Lin Liu and published by . This book was released on 1999 with total page 242 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author : Hong Shen
Publisher :
ISBN 13 :
Total Pages : 328 pages
Book Rating : 4.:/5 (396 download)
Download or read book Analysis of Proteins and Peptides by On-column Preconcentration Capillary Liquid Chromatography and Capillary Electrophoresis written by Hong Shen and published by . This book was released on 1998 with total page 328 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author : Andras Guttman
Publisher : Newnes
ISBN 13 : 0080931359
Total Pages : 391 pages
Book Rating : 4.0/5 (89 download)
Download or read book Capillary Gel Electrophoresis written by Andras Guttman and published by Newnes. This book was released on 2021-12-04 with total page 391 pages. Available in PDF, EPUB and Kindle. Book excerpt: Capillary Gel Electrophoresis and Related Microseparation Techniques covers all theoretical and practical aspects of capillary gel electrophoresis. It also provides an excellent overview of the key application areas of nucleic acid, protein and complex carbohydrate analysis, affinity-based methodologies, micropreparative aspects and related microseparation methods. It not only gives readers a better understanding of how to utilize this technology, but also provides insights into how to determine which method will provide the best technical solutions to particular problems. This book can also serve as a textbook for undergraduate and graduate courses in analytical chemistry, analytical biochemistry, molecular biology and biotechnology courses. Covers all theoretical and practical aspects of capillary gel electrophoresis Excellent overview of the key applications of nucleic acid, protein and complex carbohydrate analysis, affinity-based methodologies, micropreparative aspects and related microseparation methods Teaches readers how to use the technology and select methods that are ideal for fundamental problems Can serve as a textbook for undergraduate and graduate courses in analytical chemistry, analytical biochemistry, molecular biology and biotechnology courses
Author :
Publisher :
ISBN 13 :
Total Pages : 49 pages
Book Rating : 4.:/5 (932 download)
Download or read book Capillary Electrophoresis Fractionation, Enzyme Chemistry and Mass Spectrometry for Sensitive Detection and Identification of Peptides and Proteins: written by and published by . This book was released on 2001 with total page 49 pages. Available in PDF, EPUB and Kindle. Book excerpt: This report describes work carried out in a project with the long term goal of developing an advanced mass spectrometric method combined with separation & enzyme chemistry for the detection & identification of agents of biological origin. Part 1 covers initial efforts in demonstrating the possibility of combining capillary electrophoresis (CE) fractionation with enzyme digestion, followed by matrix-assisted laser desorption ionization/mass spectrometric (MALDI-MS)analysis of the digest for peptide mass mapping for protein identification. Part 2 presents a study that used a surfactant-treated capillary for efficient CE separation of proteins. Comparison of this method with other techniques is also discussed. The last part describes the development & application of CE/MALDI-MS for complex protein sample analysis and discusses some planned future work.
Author : Edwin Louis Gump
Publisher :
ISBN 13 :
Total Pages : 546 pages
Book Rating : 4.3/5 (121 download)
Download or read book Analysis of Proteins and Peptides Using Microcolumn Analytical Methods written by Edwin Louis Gump and published by . This book was released on 1998 with total page 546 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author : Kimberly Fay Greve
Publisher :
ISBN 13 :
Total Pages : 330 pages
Book Rating : 4.:/5 (331 download)
Download or read book Separation of Peptides and Proteins by Capillary Electrophoresis written by Kimberly Fay Greve and published by . This book was released on 1995 with total page 330 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author : Rawi Ramautar
Publisher : John Wiley & Sons
ISBN 13 : 3527833102
Total Pages : 404 pages
Book Rating : 4.5/5 (278 download)
Download or read book Capillary Electrophoresis - Mass Spectrometry for Proteomics and Metabolomics written by Rawi Ramautar and published by John Wiley & Sons. This book was released on 2022-10-03 with total page 404 pages. Available in PDF, EPUB and Kindle. Book excerpt: Capillary Electrophoresis—Mass Spectrometry for Proteomics and Metabolomics A powerful and essential resource for researchers with an interest in CE-MS In Capillary Electrophoresis—Mass Spectrometry for Proteomics and Metabolomics: Principles and Applications, a team of distinguished researchers delivers a comprehensive overview of bioanalytical capillary electrophoresis coupled to mass spectrometry (CE-MS). The book explains foundational principles, technology as well the strategies and techniques used in data analysis for metabolic and proteomic studies. It also provides a global overview of recent developments and advances for improving CE-MS sensitivity and reproducibility. An essential handbook for everyone performing metabolomic and proteomic analysis, the information provided here will assist researchers in tapping into the full potential of this technique to answer biological and clinical questions. Readers will also find: A thorough introduction to the principles of capillary electrophoresis, including its fundamentals, CE separation modes, capillary coatings, and the fundamentals of mass spectrometry In-depth examinations of technological developments in capillary electrophoresis, including sample preparation, online preconcentration, detection sensitivity, and metabolic coverage Comprehensive discussions of metabolomic studies, including their biomedical and clinical applications Recent advances in proteomics, including top-down and bottom-up approaches Perfect for analytical and clinical chemists, Capillary Electrophoresis—Mass Spectrometry for Proteomics and Metabolomics: Principles and Applications will also earn a place in the libraries of biochemists, molecular biologists, and other molecular life scientists.
Author : B. D. Hames
Publisher : OUP Oxford
ISBN 13 : 0191565636
Total Pages : 378 pages
Book Rating : 4.1/5 (915 download)
Download or read book Gel Electrophoresis of Proteins written by B. D. Hames and published by OUP Oxford. This book was released on 1998-10-01 with total page 378 pages. Available in PDF, EPUB and Kindle. Book excerpt: This new edition of Gel Electrophoresis of Proteins is a completely new text, with eight of the ten chapters written by new authors. It presents the best methods, hints and tips for core procedures such as one- dimensional polyacrylamide gel electrophoresis, isoelectric focusing, two-dimensional gel electrophoresis, preparative gel electrophoresis, and peptide mapping, complete with the latest refinements and updates of the procedures. In addition, it describes major new techniques which have come to the fore since the previous edition. Thus there are chapters on capillary gel electrophoresis, sequence analysis of gel-resolved proteins, fluorophore-labelled saccharide electrophoresis, and analysis of protein:protein interactions by gel electrophoresis. One thing has not changed. The emphasis is still on describing the best methods, in step-by-step detail, with copious advice to ensure that each method works first time in the reader's hands. The first two editions of Gel Electrophoresis of Proteins: A Practical Approach each gained a strong reputation as easy-to-follow laboratory manuals written by experienced researchers for researchers. The methods were presented in a clear accessible format and had been fully tested to ensure success in the lab. This new edition will strengthen the reputation of the book still further. It is a 'must have' for all those who currently use gel electrophoresis or who plan to do so.
