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Author : Ann Marie Staub Carrick
Publisher :
ISBN 13 :
Total Pages : 210 pages
Book Rating : 4.3/5 ( download)
Download or read book Towards Structural Analysis of Chemokine written by Ann Marie Staub Carrick and published by . This book was released on 2009 with total page 210 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author : Anna-Katharina Apel
Publisher :
ISBN 13 :
Total Pages : pages
Book Rating : 4.:/5 (113 download)
Download or read book Biophysical and Structural Analysis of the Chemokine Receptors CCR2A and CCR3, Leading to a 2.7 Å Resolution Structure of CCR2A written by Anna-Katharina Apel and published by . This book was released on 2017 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author : Catherina L. Salanga
Publisher :
ISBN 13 : 9781124703862
Total Pages : 288 pages
Book Rating : 4.7/5 (38 download)
Download or read book Structural and Functional Analysis of Chemokine Interactions with Glycosaminoglycans and Chemokine Receptors written by Catherina L. Salanga and published by . This book was released on 2011 with total page 288 pages. Available in PDF, EPUB and Kindle. Book excerpt: Chemokines are involved in cell migration and activation during routine immune surveillance, inflammation and even cancer metastasis. The migration of chemokine receptor-bearing cells, including leukocytes and tumor cells, occurs in response to the secretion of chemokines, which accumulate on cell surfaces through interaction with glycosaminoglycans (GAGs) where they effectively serve as traffic signals to guide cell movement. Engagement of chemokines with their receptors subsequently causes the activation of signaling pathways that result in firm adhesion and extravasation of the cell into tissue, and in the case of leukocytes, activation of defense mechanisms. However, in cancer cells, the signaling pathways can be exploited or redirected, resulting in responses like survival, growth and proliferation. Herein, a structural and functional approach was used to address specific questions about the interactions of chemokines (i) with GAGs and (ii) with chemokine receptors in the context of cancer. Technically, the use of mass spectrometry has been a strong theme throughout these studies. In Chapter 2, a novel application of hydroxyl radical footprinting coupled with mass spectrometry was used to characterize the GAG binding specificity of the chemokine, MCP-3/CCL7. Potential GAG binding epitopes, identified by mass spectrometry, were then validated by mutagenesis and functional assays. In Chapter 3 and 4, a phosphoproteomic mass spectrometry strategy was used to elucidate CXCL12-mediated survival signaling through the receptor, CXCR4, in cells from patients with chronic lymphocytic leukemia (CLL). While signaling cascades involved in chemokine-mediated migration are well established, pathways involved in cell survival and proliferation in cancer, are not. Methods developed for phosphopeptide enrichment, and subsequent analysis via mass spectrometry are described in Chapter 3, and interesting/novel phosphoproteins, potentially involved in CXCL12-mediated CLL survival are described in Chapter 4. In Chapter 5, a functional approach was taken to elucidate the roles of receptors CXCR4 and CXCR7 in breast cancer growth and metastasis. The data show that CXCR7 affects the functional activity of CXCR4 in vitro, and decreases the extent of lung metastases in vivo, without inhibiting primary tumor growth. Overall, these studies serve to better understand some of the regulatory mechanisms that control chemokine function in normal physiology and in cancer.
![Download Structural and Functional Analysis of the Human [beta]-chemokine I-309 PDF Online Free](https://books.google.com/books/content/images/frontcover/f2CGtgAACAAJ?fife=w200-h370)
Author : John Frank Paolini
Publisher :
ISBN 13 :
Total Pages : 476 pages
Book Rating : 4.:/5 (33 download)
Download or read book Structural and Functional Analysis of the Human [beta]-chemokine I-309 written by John Frank Paolini and published by . This book was released on 1995 with total page 476 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author : Jean-Paul Renaud
Publisher : John Wiley & Sons
ISBN 13 : 1118900502
Total Pages : 1367 pages
Book Rating : 4.1/5 (189 download)
Download or read book Structural Biology in Drug Discovery written by Jean-Paul Renaud and published by John Wiley & Sons. This book was released on 2020-01-09 with total page 1367 pages. Available in PDF, EPUB and Kindle. Book excerpt: With the most comprehensive and up-to-date overview of structure-based drug discovery covering both experimental and computational approaches, Structural Biology in Drug Discovery: Methods, Techniques, and Practices describes principles, methods, applications, and emerging paradigms of structural biology as a tool for more efficient drug development. Coverage includes successful examples, academic and industry insights, novel concepts, and advances in a rapidly evolving field. The combined chapters, by authors writing from the frontlines of structural biology and drug discovery, give readers a valuable reference and resource that: Presents the benefits, limitations, and potentiality of major techniques in the field such as X-ray crystallography, NMR, neutron crystallography, cryo-EM, mass spectrometry and other biophysical techniques, and computational structural biology Includes detailed chapters on druggability, allostery, complementary use of thermodynamic and kinetic information, and powerful approaches such as structural chemogenomics and fragment-based drug design Emphasizes the need for the in-depth biophysical characterization of protein targets as well as of therapeutic proteins, and for a thorough quality assessment of experimental structures Illustrates advances in the field of established therapeutic targets like kinases, serine proteinases, GPCRs, and epigenetic proteins, and of more challenging ones like protein-protein interactions and intrinsically disordered proteins
Author : Ariane L. Jansma
Publisher :
ISBN 13 :
Total Pages : 206 pages
Book Rating : 4.:/5 (55 download)
Download or read book Structural and Functional Analysis of the Chemokine CCL27 and the Expression and Purification of Silent Chemokine Receptors D6 and DARC written by Ariane L. Jansma and published by . This book was released on 2009 with total page 206 pages. Available in PDF, EPUB and Kindle. Book excerpt: Chemokines are small chemoattractant proteins that function by binding to G-protein coupled receptors (GPCRs) on a wide variety of cell types, triggering cascades of intracellular signaling pathways. While they are best known for their role in leukocyte migration, both in standard immune surveillance and maintenance, as well as in response to inflammation, chemokines are involved in a variety of physiological and pathophysiological processes. In addition to their complex function, the chemokine network itself is highly complex, with some chemokines being specific to one receptor, while others activate multiple receptors expressed on different cell types and in some cases, resulting in very different cellular responses. This project in part involves the chemokine CCL27, which is expressed in skin and selectively chemoattracts CLA memory T cells expressing the chemokine receptor, CCR10. The first set of aims for this project involved a comprehensive analysis of the structural and functional mechanism contributing to the biological diversity of CCL27. This was accomplished through a biophysical characterization of the oligomerization properties, residues targeting receptor activation, as well as sites of glycosaminoglycan (GAG) interactions for this chemokine. The results suggest that CCL27 exists in multiple oligomeric states, and its unique oligomerization patterns appear to play a role in the diversity of its multiple binding partners. The second major project aim involves optimization of the expression and purification of the silent chemokine receptors D6 and the Duffy Antigen Receptor for Chemokines (DARC). D6 and DARC are termed silent receptors because they bind many chemokines with high affinity and specificity, but unlike other chemokine receptors, they do not signal through G-proteins. Instead, they act as regulators, either by targeting their chemokine ligands for degradation, or by shuttling them from one location to another. One of the rate-limiting steps in the biophysical characterization of 7-transmembrane helical chemokine receptors is obtaining sufficient amounts of purified, functional protein. In this project, a tetracycline-indicuble mammalian cell expression system is applied to both D6 and DARC. Results from the initial test expressions and purifications indicate that this method was successful in generating solubilized receptor protein.
Author : Michael Corbett
Publisher :
ISBN 13 :
Total Pages : 224 pages
Book Rating : 4.:/5 (9 download)
Download or read book Molecular and Structural Analysis of Natural Variants of Parapoxvirus Chemokine Binding Proteins Reveals a Diverse Range of Chemokine Class Specificity written by Michael Corbett and published by . This book was released on 2013 with total page 224 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author : Polina Isaikina
Publisher :
ISBN 13 :
Total Pages : 0 pages
Book Rating : 4.:/5 (139 download)
Download or read book Structural and Functional Analysis of the Human Chemokine Receptor and HIV-1 Co-receptor CCR5 written by Polina Isaikina and published by . This book was released on 2023 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author : Hae Ryong Kwon
Publisher :
ISBN 13 :
Total Pages : 120 pages
Book Rating : 4.:/5 (71 download)
Download or read book Study of the Structure and Function of CXC Chemokine Receptor 2 written by Hae Ryong Kwon and published by . This book was released on 2010 with total page 120 pages. Available in PDF, EPUB and Kindle. Book excerpt: It has been shown that the amino terminus and second extracellular loop (EC2) of CXCR2 are crucial for ligand binding and receptor activation. The lack of an ionic lock motif in the third intracellular loop of CXCR2 focuses an investigation of the mechanism by which these two extracellular regions contribute to receptor recognition and activation. The first objective of this investigation was to predict the structure of CXCR2 based on known structures of crystallized GPCRs. Rhodopsin, [beta]2- adrenergic receptor, CXCR4 were used for homology modeling of CXCR2 structure. Highly conserved motifs found in sequence alignments of the template GPCRs were helpful to generate CXCR2 models. We also studied solvent accessibility of residues in the EC2 of CXCR2 in the inactive state. Most of the residues in the EC2 were found to be solvent accessible in the inactive state, suggesting the residues might be involved in ligand recognition. Second, we studied the role of charged residues in the EC2 of CXCR2 in ligand binding and receptor activation using constitutively active mutants (CAM) of CXCR2, D9K and D9R. Combinatorial mutations consisting of the CAM in the amino terminus and single mutations of charged residues in the EC2 were generated to study two concepts including "attraction" and "repulsion" models. The mutant receptors were used to test their effects on cell surface expression, ligand binding, receptor activation through PLC-[beta]3, and cellular transformation. All the mutations in the repulsion model result in CXCR2 receptors that are unable to bind ligand, suggesting that each of the Arg residues in the EC2 are important for ligand recognition. Interestingly, mutations in the attraction model partially inhibited receptor activation by the CAM D9K, suggesting that Glu198 and Asp199 residues in the EC2 are associated with receptor activation. Furthermore, a novel CAM, E198A/D199A, was identified in this study. These negatively charged residues are very close to a conserved disulfide bond linking the EC2 and the third transmembrane. In this sense, these current discoveries concerning the structural basis of CXCR2 and interdisciplinary approaches would provide new insights to investigate unknown mechanisms of interaction with its cognate ligands and receptor activation.
Author : Christian Taylor Gilliland
Publisher :
ISBN 13 : 9781303616686
Total Pages : 232 pages
Book Rating : 4.6/5 (166 download)
Download or read book Structural and Functional Analysis of the Constitutively Active C-C Chemokine Receptor Type 1 (CCR1) written by Christian Taylor Gilliland and published by . This book was released on 2013 with total page 232 pages. Available in PDF, EPUB and Kindle. Book excerpt: Chemokine receptors belong to the G protein-coupled receptor (GPCR) family of proteins and are critical mediators of the directed migration of leukocytes in innate and adaptive immune responses. Understanding the behavior of chemokine receptors under basal and agonist-stimulated conditions is essential to developing effective therapeutics for inflammatory and autoimmune diseases. For the first time, the constitutive activity of the C-C chemokine receptor type 1 (CCR1) is uncovered through ligand-independent cellular migration, constitutive phosphorylation and association with [beta]-arrestin-2, and continual internalization followed by recycling back to the plasma membrane. Initial data suggests that CCR1 can act as a scavenging receptor to sequester chemokines intracellularly without canonical G protein signaling, thereby providing biological relevance to receptor constitutive activity. A Ser/Thr-rich cluster in the distal carboxy-terminal tail of CCR1 is identified as the major site of basal phosphorylation and fulfills a necessary, but not sufficient, role in pre-coupling to [beta]-arrestin-2. Site-directed mutagenesis of receptor transmembrane domains and conserved DRY motif has identified residues important for stabilizing CCR1 in a constitutively active state. Activation of CCR1 primarily leads to a conformational rearrangement with [beta]-arrestin-2, while endogenous chemokines induce this change with differential potency and efficacy. Lastly, small metal ion chelator molecules are able to activate desensitization and down-modulation of CCR1 with similar efficacy to natural ligands. Taken together, the work presented herein underlies the complexity of CCR1 function in the presence and absence of ligand and provides new avenues for therapeutic targeting.
Author :
Publisher : Academic Press
ISBN 13 : 9780121827991
Total Pages : 672 pages
Book Rating : 4.8/5 (279 download)
Download or read book Nuclear Magnetic Resonance of Biological Macromolecules, Part C written by and published by Academic Press. This book was released on 2005-04-20 with total page 672 pages. Available in PDF, EPUB and Kindle. Book excerpt: The critically acclaimed laboratory standard, Methods in Enzymology, is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. The series contains much material still relevant today - truly an essential publication for researchers in all fields of life sciences. Nuclear Magnetic Resonance of Biological Macromolecules, Part C is written with a "hands-on" perspective. That is, practical applications with critical evaluations of methodologies and experimental considerations needed to design, execute, and interpret NMR experiments pertinent to biological molecules. * One of the most highly respected publications in the field of biochemistry since 1955 * Frequently consulted, and praised by researchers and reviewers alike * Truly an essential publication for anyone in any field of the life sciences
Author : Jeffrey K. Harrison
Publisher : Springer Science & Business Media
ISBN 13 : 1597450200
Total Pages : 412 pages
Book Rating : 4.5/5 (974 download)
Download or read book The Chemokine Receptors written by Jeffrey K. Harrison and published by Springer Science & Business Media. This book was released on 2007-11-17 with total page 412 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume, new to The Receptors series, focuses on several areas, including the birth, maturation, and structure of Chemokines; Neutrophil, Dendritic, and Lymphocyte trafficking; and Chemokine Receptors in diseases such as AIDs and lung cancer. In particular the book contains cutting-edge information ranging from basic molecular and cellular mechanisms to physiological and pathological roles of chemokines.
Author : Barry S. Selinsky
Publisher : Springer Science & Business Media
ISBN 13 : 159259400X
Total Pages : 330 pages
Book Rating : 4.5/5 (925 download)
Download or read book Membrane Protein Protocols written by Barry S. Selinsky and published by Springer Science & Business Media. This book was released on 2008-02-03 with total page 330 pages. Available in PDF, EPUB and Kindle. Book excerpt: Knowledge of the three-dimensional structure of a protein is absolutely required for the complete understanding of its function. The spatial orientation of amino acids in the active site of an enzyme demonstrates how substrate specificity is defined, and assists the medicinal chemist in the design of s- cific, tight-binding inhibitors. The shape and contour of a protein surface hints at its interaction with other proteins and with its environment. Structural ana- sis of multiprotein complexes helps to define the role and interaction of each individual component, and can predict the consequences of protein mutation or conditions that promote dissociation and rearrangement of the complex. Determining the three-dimensional structure of a protein requires milligram quantities of pure material. Such quantities are required to refine crystallization conditions for X-ray analysis, or to overcome the sensitivity limitations of NMR spectroscopy. Historically, structural determination of proteins was limited to those expressed naturally in large amounts, or derived from a tissue or cell source inexpensive enough to warrant the use of large quantities of cells. H- ever, with the advent of the techniques of modern gene expression, many p- teins that are constitutively expressed in minute amounts can become accessible to large-scale purification and structural analysis.
Author : Sangdun Choi
Publisher : Springer
ISBN 13 : 9781493968008
Total Pages : 6330 pages
Book Rating : 4.9/5 (68 download)
Download or read book Encyclopedia of Signaling Molecules written by Sangdun Choi and published by Springer. This book was released on 2017-12-15 with total page 6330 pages. Available in PDF, EPUB and Kindle. Book excerpt: The second edition of this encyclopedia presents over 400 biologically important signaling molecules and the content is built on the core concepts of their functions along with early findings written by some of the world’s foremost experts. The molecules are described by recognized leaders in each molecule. The interactions of these single molecules in signal transduction networks will also be explored. This encyclopedia marks a new era in overview of current cellular signaling molecules for the specialist and the interested non-specialist alike. Currently, there are more than 30,000 genes in human genome. However, not all the proteins encoded by these genes work equally in order to maintain homeostasis. Understanding the important signaling molecules as completely as possible will significantly improve our research-based teaching and scientific capabilities.
Author : Oystein Bruserud
Publisher : Springer Science & Business Media
ISBN 13 : 3642126391
Total Pages : 214 pages
Book Rating : 4.6/5 (421 download)
Download or read book The Chemokine System in Experimental and Clinical Hematology written by Oystein Bruserud and published by Springer Science & Business Media. This book was released on 2010-09-30 with total page 214 pages. Available in PDF, EPUB and Kindle. Book excerpt: The aim of the issue is to describe and explain the importance of the chemokine system in hematology. The chemokine system is probably important for many aspects of normal as well as malignant hematopoiesis. A major focus is the development and treatment of hematologic malignancies, including the immunobiology of stem cell transplantation. The present reviews illustrate that chemokines can be involved in leukemogenesis. The chemokine system is also important both for the crosstalk between malignant cells and their neighbouring nonmalignant stromal cells (including endothelial cells) as well as for immunoregulation in patients treated with allogeneic stem cell transplantation. Thus, chemokines are important both for the pathogenesis and treatment of hematological diseases.
Author : Martin J. Stone
Publisher : MDPI
ISBN 13 : 3038427284
Total Pages : 229 pages
Book Rating : 4.0/5 (384 download)
Download or read book Regulation of Chemokine- Receptor Interactions and Functions written by Martin J. Stone and published by MDPI. This book was released on 2018-03-19 with total page 229 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book is a printed edition of the Special Issue "Regulation of Chemokine-Receptor Interactions and Functions" that was published in IJMS
Author : David Malcolm James Lilley
Publisher : Royal Society of Chemistry
ISBN 13 : 0854042539
Total Pages : 339 pages
Book Rating : 4.8/5 (54 download)
Download or read book Ribozymes and RNA Catalysis written by David Malcolm James Lilley and published by Royal Society of Chemistry. This book was released on 2008 with total page 339 pages. Available in PDF, EPUB and Kindle. Book excerpt: Takes the reader through the origins of catalysis in RNA and necessarily includes significant discussion of structure and folding. The main focus of the book concerns chemical mechanism with extensive comment on how, despite the importance of RNA catalysis in the cell, its origins are still poorly understood and often controversial. The reader is given an outline of the important role of RNA catalysis in many aspects of cell function, including RNA processing and translation.
