Thysanoptera Studies

Author : Alvah Peterson
Publisher : Forgotten Books
ISBN 13 : 9781334745713
Total Pages : 58 pages
Book Rating : 4.7/5 (457 download)

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Download or read book Morphological Studies on the Head and Mouth-Parts of the Thysanoptera (Classic Reprint) written by Alvah Peterson and published by Forgotten Books. This book was released on 2016-12-22 with total page 58 pages. Available in PDF, EPUB and Kindle. Book excerpt: Excerpt from Morphological Studies on the Head and Mouth-Parts of the Thysanoptera For general purposes thrips should be killed in boiling water and preserved in 70 per cent. Alcohol. When only the chitinized parts are desired, living or preserved specimens should be boiled for ten or fifteen minutes in a 10 per cent. Solution of potassium hydroxide, then reboiled in water to remove the alkali, and finally preserved in 70 per cent. Alcohol. Living material treated in the above manner is better than preserved material, for preservation in alcohol tends to make the specimens too brittle for careful dissection. The use of a Leitz binocular microscope made possible the dissection of the minute mouth-parts. A number of media were tried in which to make dissections; carbol-aniline oil proved to be the best. Its good qualities are that it evaporates slowly and will clear specimens from any grade of alcohol above 50 per cent: If it be desirable to stain with safranin or orange G the stain can be dissolved in 95 per cent. Alcohol and will readily mix with the carbol-aniline oil. The staining of the material for dissection with safranin proved to be very useful in differentiating the almost colorless mouth-parts of some of the species. In using aniline oil in any form one precaution must be observed; as much as possible of the oil should be removed with a blotting paper or a dry rag or by replacing the carbol-aniline oil with carbol-xylene or xylene before mounting in balsam. If the oil be not removed the media in which the parts are immersed will eventually darken. Material for sections was fixed with hot (80 C.) corrosive sublimate (saturated corrosive sublimate in 35% alcohol plus 2% of glacial acetic acid) for 15 to 30 minutes, which was replaced by 70% alcohol containing a few drops of iodine for 24 or more hours. About the Publisher Forgotten Books publishes hundreds of thousands of rare and classic books. Find more at www.forgottenbooks.com This book is a reproduction of an important historical work. Forgotten Books uses state-of-the-art technology to digitally reconstruct the work, preserving the original format whilst repairing imperfections present in the aged copy. In rare cases, an imperfection in the original, such as a blemish or missing page, may be replicated in our edition. We do, however, repair the vast majority of imperfections successfully; any imperfections that remain are intentionally left to preserve the state of such historical works."