Read Books Online and Download eBooks, EPub, PDF, Mobi, Kindle, Text Full Free.
Download Three Dimensional Microvascular Networks For Tissue Engineering full books in PDF, epub, and Kindle. Read online Three Dimensional Microvascular Networks For Tissue Engineering ebook anywhere anytime directly on your device. Fast Download speed and no annoying ads. We cannot guarantee that every ebooks is available!
Author : Taylor M. Simpson
Publisher :
ISBN 13 :
Total Pages : 152 pages
Book Rating : 4.:/5 (777 download)
Download or read book Three-dimensional Microvascular Networks for Tissue Engineering written by Taylor M. Simpson and published by . This book was released on 2006 with total page 152 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author : Carlos Chang
Publisher :
ISBN 13 :
Total Pages : 490 pages
Book Rating : 4.:/5 (659 download)
Download or read book Development of In Vitro Three-Dimensional Microvascular Tissues written by Carlos Chang and published by . This book was released on 2008 with total page 490 pages. Available in PDF, EPUB and Kindle. Book excerpt: Microvasculatures may become damaged by a variety of acute and chronic diseases. In many cases, microvessel function is irreversibly compromised, leading to the dysfunction and even death of surrounding tissues. Currently, there are few therapies that directly address the treatment of microvascular insufficiency. Responding to this need, researchers are developing methods to fabricate in vitro blood vessels. Typical strategies include; cellular sodding within polymers and/or biopolymers, the formation of cylindri-cal cellular monolayers around polymer mandrels, and the modification of biocompatible surfaces for cellular adhesion. Using currently available techniques, simple, individual vessel conduits have been engineered with internal diameters down to 150æ̐m. However, no evidence has been provided illustrating the formation of patent, interconnected mi-crovessel networks without the aid of a host circulatory system. In response to this challenge, it is hypothesized that a novel flow-based experimen-tal system will support the in vitro development of three-dimensional microvascular tis-sues. Addressing this hypothesis, the presented work focused on three specific aims: Spe-cific Aim 1. Pattern planar in vitro three-dimensional microvasculatures. Specific Aim 2. Engineer a Dynamic In vitro Perfusion Chamber (DIP Chamber) for microvascular inves-tigation. Specific Aim 3. In vitro perfusion of microvessel fragments within the DIP Chamber. Through the supporting experiments, directed endothelial sprouting from par-ent isolated microvessel fragments was achieved. In addition, patent in vitro microvessel networks were successfully developed. The presented experiments are the first to achieve these experimental results. In addition, the described experimental model will provide a unique method for future investigations of microcirculatory phenomena. Since no exoge-nous growth factors or cell signals were introduced into the constructs, it is believed that this system presents a physiological platform for future investigations into angiogenesis, angioadaptation, and network remodeling. Moreover, this model may offer a useful plat-form for vascular therapeutic testing and a foundation for future tissue engineering applications.
Author : Eric M. Brey
Publisher : CRC Press
ISBN 13 : 1466580453
Total Pages : 402 pages
Book Rating : 4.4/5 (665 download)
Download or read book Vascularization written by Eric M. Brey and published by CRC Press. This book was released on 2014-08-07 with total page 402 pages. Available in PDF, EPUB and Kindle. Book excerpt: A Complex and Growing Field The study of vascularization in tissue engineering and regenerative medicine (TERM) and its applications is an emerging field that could revolutionize medical approaches for organ and tissue replacement, reconstruction, and regeneration. Designed specifically for researchers in TERM fields, Vascularization: Regenerative Medicine and Tissue Engineering provides a broad overview of vascularization in TERM applications. This text summarizes research in several areas, and includes contributions from leading experts in the field. It defines the difficulties associated with multicellular processes in vascularization and cell-source issues. It presents advanced biomaterial design strategies for control of vascular network formation and in silico models designed to provide insight not possible in experimental systems. It also examines imaging methods that are critical to understanding vascularization in engineered tissues, and addresses vascularization issues within the context of specific tissue applications. This text is divided into three parts; the first section focuses on the basics of vascularization. The second section provides general approaches for promoting vascularization. The final section presents tissue and organ-specific aspects of vascularization in regenerative medicine. Presents Areas of Substantial Clinical and Societal Impact The material contains research and science on the process of vessel assembly with an emphasis on methods for controlling the process for therapeutic applications. It describes the tissue and organ-specific aspects of vascularization in regenerative medicine, and refers to areas such as bone tissue engineering, vascularization of encapsulated cells, adipose tissue, bone and muscle engineering. It also provides a mechanistic understanding of the process and presentation of experimental and computational approaches that facilitate the study of vascular assembly, and includes enabling technologies such as nanotechnology, drug delivery, stem cells, microfluidics, and biomaterial design that are optimized for supporting the formation of extensive vascular networks in regenerative medicine. A guide for researchers developing new methods for modulating vessel assembly, this text can also be used by senior undergraduate and graduate students taking courses focused on TERM.
Author : Karen J.L. Burg
Publisher : CRC Press
ISBN 13 : 1482231182
Total Pages : 412 pages
Book Rating : 4.4/5 (822 download)
Download or read book Engineering 3D Tissue Test Systems written by Karen J.L. Burg and published by CRC Press. This book was released on 2017-07-28 with total page 412 pages. Available in PDF, EPUB and Kindle. Book excerpt: Engineering 3D Tissue Test Systems provides an introduction to, and unique coverage of, a rapidly evolving area in biomaterials engineering. It reveals the current and future research responses, the current and future diagnostic applications, and provides a comprehensive overview to foster innovation. It offers insight into the importance of 3D systems and their use as benchtop models, spanning applications from basic scientific research to clinical diagnostics. Methods and limitations of building 3D tissue structures are evaluated, with attention given to the cellular, polymeric, and fabrication instrumentation components. The book covers the important aspects of polymeric tissue test systems, highlighting the needs and constraints of the industry, and includes a chapter on regulatory and pricing issues.
Author : Wolfgang Holnthoner
Publisher : Springer
ISBN 13 : 9783319545844
Total Pages : 0 pages
Book Rating : 4.5/5 (458 download)
Download or read book Vascularization for Tissue Engineering and Regenerative Medicine written by Wolfgang Holnthoner and published by Springer. This book was released on 2021-06-09 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: This reference work presents the basic principles of angiogenesis induction, including the roles of signaling factors such as hypoxia-inducible factors, biophysical stimulation and angiogenic cells. The book also covers lymphogenesis induction. Both the established fundamentals in the field as well as new trends in the vascularization of engineered tissues are discussed. These include pre-vascularization strategies using preparation of channeled scaffolds and preparation of decellularized blood vessel trees, approaches to inducing formation of microvasculature and approaches to inducing the growth of vascular networks. The authors expand on these concepts with current studies of dual-level approaches to engineer vascularized tissue composites. The book concludes with a discussion of current clinical approaches and the use of vascular grafts in the context of providing clinical practice with new tissue engineering strategies.
Author : Xiaolu Zhu
Publisher : CRC Press
ISBN 13 : 1000400840
Total Pages : 142 pages
Book Rating : 4.0/5 (4 download)
Download or read book Self-Organized 3D Tissue Patterns written by Xiaolu Zhu and published by CRC Press. This book was released on 2022-05-26 with total page 142 pages. Available in PDF, EPUB and Kindle. Book excerpt: Therapies for regenerating damaged tissue and organs have been attracting much attention. In order to efficiently regenerate the functions of living tissue and organs, diverse attempts have been made to utilize scaffolds to “mold” artificial tissue structures. However, the structural complexity of reconstituted tissue is limited by the mechanical precision of scaffolds, which still cause problems arising from their degradation, immunogenic reactions, and so forth. It is also being realized that ultimately the best approach might be to rely on the innate self-organizing properties of cells and the regenerative capability of the organism itself. This book investigates the 3D-pattern formation and evolution mechanism in multipotent cells embedded in 3D semi-synthetic hydrogels and the control methodology for self-organized patterns. The authors theoretically and experimentally demonstrate several types of topological 3D-pattern formation by cells in a 3D matrix in vitro, which can be modeled and predicted by mathematical models based on the reaction-diffusion dynamics of various chemical, physical, and mechanical cues. The study, focused on the 3D pattern formation of cells, provides (i) a unique perspective for understanding the self-organized 3D tissue structures based on Turing instability, (ii) the scheme for rationally controlling the cellular self-organization via exogenous factors or tailored inner interfaces inside hydrogels, and (iii) the elaborate and sophisticated regulating method for tuning collective cellular behaviors in 3D matrices.
Author : Eric S. Comeau
Publisher :
ISBN 13 :
Total Pages : 184 pages
Book Rating : 4.:/5 (113 download)
Download or read book Ultrasound Standing Wave Field Technology for Cell Patterning and Microvessel Network Formation in Vitro and in Situ written by Eric S. Comeau and published by . This book was released on 2017 with total page 184 pages. Available in PDF, EPUB and Kindle. Book excerpt: "The field of tissue engineering holds great promise to regenerate or repair diseased and damaged tissues. However, the fabrication of thick, three-dimensional (3D) tissues is currently limited by the need for perfused microvascular networks to volumetrically supply tissues with nutrients and oxygen. Current efforts to engineer microvascular networks have drawbacks including the inability to control vessel morphology, a limited potential for volumetric scale up, or incompatibility for translation in vivo. The research reported in this thesis developed ultrasound standing wave field (USWF) technologies to volumetrically pattern cells in 3D collagen gels for the fabrication of microvascular networks in vitro and in situ. USWF cell patterning parameters were shown to influence initial 3D endothelial cell patterning, and subsequently, the resultant microvascular network morphology in vitro. USWFs were also employed to co-pattern endothelial and smooth muscle cell types to direct microvascular network morphology, enhance network viability, and promote the formation of mural cell-invested microvessels. Furthermore, novel USWF exposure systems were constructed to demonstrate the feasibility of patterning particles and cells for tissue engineering and regenerative medicine applications in vivo. USWF cell patterning has great capacity to generate vascularized microphysiological systems in vitro, as well as promote tissue regeneration in vivo. Further development of USWF cell patterning technologies would provide noninvasive, rapid, and transformative procedures for the field of tissue engineering."--Page xi.
Author : Hatice Genç
Publisher :
ISBN 13 :
Total Pages : 0 pages
Book Rating : 4.:/5 (138 download)
Download or read book Perfusable Microvascular Networks for Biofabrication of Three-dimensional Tissue Models: Channel Endothelialization and Biofunctional Characterization of Constructs written by Hatice Genç and published by . This book was released on 2023 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author : Masaki Ieda
Publisher : Springer
ISBN 13 : 3319561065
Total Pages : 274 pages
Book Rating : 4.3/5 (195 download)
Download or read book Cardiac Regeneration written by Masaki Ieda and published by Springer. This book was released on 2017-10-27 with total page 274 pages. Available in PDF, EPUB and Kindle. Book excerpt: This Volume of the series Cardiac and Vascular Biology offers a comprehensive and exciting, state-of-the-art work on the current options and potentials of cardiac regeneration and repair. Several techniques and approaches have been developed for heart failure repair: direct injection of cells, programming of scar tissue into functional myocardium, and tissue-engineered heart muscle support. The book introduces the rationale for these different approaches in cell-based heart regeneration and discusses the most important considerations for clinical translation. Expert authors discuss when, why, and how heart muscle can be salvaged. The book represents a valuable resource for stem cell researchers, cardiologists, bioengineers, and biomedical scientists studying cardiac function and regeneration.
Author : Mohammed Assad Yousefhussien
Publisher :
ISBN 13 :
Total Pages : 108 pages
Book Rating : 4.:/5 (828 download)
Download or read book Three-dimensional Quantification and Visualization of Vascular Networks in Engineered Tissues written by Mohammed Assad Yousefhussien and published by . This book was released on 2012 with total page 108 pages. Available in PDF, EPUB and Kindle. Book excerpt: "Three-dimensional textural and volumetric image analysis holds great potential in understanding the image data produced by multi-photon microscopy. In this thesis, a tool that provides quantitative textural and morphometric analyses of vasculature in engineered tissues, alongside with a fast three-dimensional volume rendering is proposed. The investigated 3D artificial tissues consist of Human Umbilical Vein Endothelial Cells (HUVEC) embedded in collagen exposed to two regimes of ultrasound standing wave fields under different pressure conditions. Textural features were evaluated over the extracted connected region in our samples using the normalized Gray Level Co-occurrence Matrix (GLCM) combined with Gray-Level Run Length Matrix (GLRLM) analysis. To minimize the error resulting from any possible volumetric rotation and to provide a comprehensive textural analysis, an averaged version of nine GLCM and GLRLM orientations is used. To evaluate volumetric features, parameters such as volume run length and percentage volume were utilized. The z-projection versions of the samples were used to estimate the tortuosity of the vessels, as well as to measure the length and the angle of the branches. We utilized a three-dimensional volume rendering technique named MATVTK (derived from MATLAB and VTK) and runs under MATLAB that shows a great improvement on the processing time to reconstruct our volumes compared to MATLAB built-in functions. Results show that our analysis is able to differentiate among the exposed samples, due to morphological changes induced by the ultrasound standing wave fields. Furthermore, we demonstrate that providing more textual parameters than what is currently being reported in the literature, enhances the quantitative understanding of the heterogeneity of artificial tissues."--Abstract.
Author : Xiaofang Chen
Publisher :
ISBN 13 : 9781109307580
Total Pages : 91 pages
Book Rating : 4.3/5 (75 download)
Download or read book Engineering Three-dimensional Vessel Networks written by Xiaofang Chen and published by . This book was released on 2009 with total page 91 pages. Available in PDF, EPUB and Kindle. Book excerpt: The survival of thick artificial tissue in vivo requires the formation of three dimensional vessel networks that can anastomose with host vasculature and transport blood to the central part of the tissue quickly after implantation. In this study, a prevascularization strategy was used to create vessel networks in the engineered tissue constructs. The prevascularized tissue model was developed by co-culture of human umbilical vein endothelial cells (HUVECs) and fibroblasts in a fibrin gel. Interconnected vessel networks with visible lumens were developed within the tissue construct in 1 week. In order to demonstrate whether the preformed vessel networks can be functional in vivo, the prevascularized tissue as well as unprevascularized controls were implanted subcutaneously on the dorsal surface of immune-deficient mice. HUVEC-lined vessels containing red blood cells were evident in the prevascularized tissue by day 5, significantly earlier than non-prevascularized tissues (14 days). In addition, collagen deposition and larger number of proliferating cells were observed in the prevascularized tissue indicating higher level of cell viability. In order to further reduce the time required for the formation of functional anstomosis, the seeding density of HUVECs and fibroblasts were optimized. The development of vessel networks in vitro and the formation of functional anastomosis in vivo were significantly accelerated at the presence of high density of fibroblasts (2 million/ml) compared to low density of fibroblasts (0.2 million/ml). Finally, HUVECs were replaced by the endothelial cells derived from cord blood endothelial progenitor cells (EPC-ECs). Vessels formed with EPC-ECs showed similar kinetics as HUVECs in vitro. However, implantation of the prevascularized tissue construct in mice revealed a dramatic difference in the ability of EPC-ECs and HUVECs to form anastomosis with the host vasculature. Vascular beds derived from EPC-ECs were perfused within one day of implantation whereas no HUVEC vessels were perfused at day 1. Furthermore, while almost 90% of EPC-EC derived vessel networks were perfused at day 3, only one third of HUVEC derived vascular beds were perfused. These results demonstrated that prevascularization of engineered tissue constructs with well developed vessel network is an effective strategy to achieve fast perfusion of the engineered tissue constructs in vivo after implantation. This method will potentially facilitate the development of large three dimensional engineered tissues.
Author : Daniel Therriault
Publisher : Ann Arbor, Mich. : University Microfilms International
ISBN 13 :
Total Pages : 258 pages
Book Rating : 4.:/5 (55 download)
Download or read book Directed Assembly of Three Dimensional Microvascular Networks written by Daniel Therriault and published by Ann Arbor, Mich. : University Microfilms International. This book was released on 2003 with total page 258 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author : Chunxiao Cui
Publisher :
ISBN 13 :
Total Pages : 179 pages
Book Rating : 4.:/5 (123 download)
Download or read book Spatiotemporal Control of Microscopic Hydrogel Deformations and Their Tissue Engineering Applications written by Chunxiao Cui and published by . This book was released on 2020 with total page 179 pages. Available in PDF, EPUB and Kindle. Book excerpt: Tissue engineering, which aims to regenerate damaged human tissues or organs with biomaterials and living cells, developed rapidly in the past few decades and has motivated remarkable advancement in the studies on cell engineering, biomaterials, and biofabrication. In spite of such progress, the regeneration of thick tissues, which is needed for repairing a sizable tissue defect, remains difficult. One of the major obstacles is the challenge of creating cell-laden, cell-scale hollow structures that resemble the microvascular networks of our tissues. In an implant for our body, these microvascular networks enable the exchange of nutrients, oxygen, and metabolic wastes between cells and the larger blood vessels, which sustaining the cells' and the tissues' survival. In this study, I developed a novel 4D printing platform that creates microscale, cell-encapsulating, hollow 3D scaffolds for tissue vascularization. Human umbilical vein endothelial cells (HUVECs) were seeded on an inkjet-printed bilayer gelatin micropattern, the cell-lined micropattern was made capable of self-folding into 3D microtubes, at a moment of choice. This work contains four major topics including (a) developing a 4D platform to implement 4D printing of microscale cell-encapsulating hollow 3D scaffold, (b) developing a mathematical model to decipher the dynamics of shape transformation of the self-folding micropatterns, (c) developing biomimetic microvessel/microvascular network using the 4D printing method and investigating how cells behave in response to the microtubular, physical environment given by the 4D printing, and (d) testing the feasibility of vascularizing a 3D tissue-engineering scaffold using the 4D-printed microvessels. This study provides a novel way to create 3D cell-encapsulating tissue engineering scaffold and sheds the light on creating vascularized 3D tissue engineering implants as regenerative medicine.
Author : Willie Wu
Publisher :
ISBN 13 :
Total Pages : pages
Book Rating : 4.:/5 (776 download)
Download or read book Direct Ink Writing of Microvascular Networks written by Willie Wu and published by . This book was released on 2011 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: Nature is replete with examples of embedded microvascular systems that enable efficient fluid flow and distribution for autonomic healing, cooling, and energy harvesting. The ability to incorporate microvascular networks in functional materials systems is therefore both scientifically and technologically important. In this PhD thesis, the direct-write assembly of planar and 3D biomimetic microvascular networks within polymer and hydrogel matrices is demonstrated. In addition, the influence of network design of fluid transport efficiency is characterized. Planar microvascular networks composed of periodic lattices of uniformal microchannels and hierarchical, branching architectures are constructed by direct-write assembly of a fugitive organic ink. Several advancements are required to facilitate their patterning, including pressure valving, dual ink printing, and dynamic pressure variation to allow tunable control of ink deposition. The hydraulic conductance is measured using a high pressure flow meter as a function of network design. For a constant vascular volume and areal coverage, 2- and 4-generation branched architectures that obey Murray's Law exhibited the highest hydraulic conductivity. These experimental observations are in good agreement with predictions made by analytic models. 3D microvascular networks are fabricated by omnidirectional printing a fugitive organic ink into a photopolymerizable hydrogel matrix that is capped with fluid filler of nearly identical composition. Using this approach, 3D networks of arbitrary design can be patterned. After ink deposition is complete, the matrix and fluid filler are chemically cross-linked via UV irradiation, and the ink is removed by liquefication. Aqueous solutions composed of a triblock copolymer of polyethylene oxide (PEO)-polypropylene oxide (PPO)-PEO constitute the materials system of choice due to their thermal- and concentration-dependent phase behavior. Specifically, the fugitive ink consists of a 23 w/w% PEO-PPO-PEO (Pluronic F127) solution, while matrix (25 w/w%) and fluid filler (20 w/w%) are composed of an acrylate-modified form of the Pluronic F127 that can be subsequently photopolymerized. The ink and matrix concentrations exceed the critical micelle concentration (CMC) of 22 w/w% and thus reside in a physical gel state. At their respective concentrations, they possess an elastic plateau modulus G' > 104 Pa needed for ink filament formation, shape retention, and support during the printing process. By contrast, the fluid filler is formulated below the CMC to facilitate its flow into void spaces created as the nozzle translates through the matrix during printing. After printing is completed, photopolymerization is carried out to yield a chemically cross-linked matrix from which the fugitive ink is removed leaving behind the desired 3D microvascular network. Due to the potential application of 3D microvasularized hydrogels in tissue engineering, dye diffusion through the cured Pluronic F127-diacrylate matrix is investigated via fluorescent microscopy. Image analysis is used to extract diffusion profiles of the dye as a function of time. Extraction of the 1-D Gaussian fitting parameters is used to determine the spatial peak variance 1̐đ2 and plotted as a function of time to determine the dye diffusivity.
Author : Jeffrey O. Hollinger
Publisher : CRC Press
ISBN 13 : 1135501912
Total Pages : 500 pages
Book Rating : 4.1/5 (355 download)
Download or read book Bone Tissue Engineering written by Jeffrey O. Hollinger and published by CRC Press. This book was released on 2004-10-14 with total page 500 pages. Available in PDF, EPUB and Kindle. Book excerpt: Focusing on bone biology, Bone Tissue Engineering integrates basic sciences with tissue engineering. It includes contributions from world-renowned researchers and clinicians who discuss key topics such as different models and approaches to bone tissue engineering, as well as exciting clinical applications for patients. Divided into four sections, t
Author : Aldo R. Boccaccini
Publisher : Springer Science & Business Media
ISBN 13 : 3642180566
Total Pages : 272 pages
Book Rating : 4.6/5 (421 download)
Download or read book Myocardial Tissue Engineering written by Aldo R. Boccaccini and published by Springer Science & Business Media. This book was released on 2011-08-29 with total page 272 pages. Available in PDF, EPUB and Kindle. Book excerpt: Myocardial tissue engineering (MTE), a concept that intends to prolong patients’ life after cardiac damage by supporting or restoring heart function, is continuously improving. Common MTE strategies include an engineered ‘vehicle’, which may be a porous scaffold or a dense substrate or patch, made of either natural or synthetic polymeric materials. The function of the substrate is to aid transportation of cells into the diseased region of the heart and support their integration. This book, which contains chapters written by leading experts in MTE, gives a complete analysis of the area and presents the latest advances in the field. The chapters cover all relevant aspects of MTE strategies, including cell sources, specific TE techniques and biomaterials used. Many different cell types have been suggested for cell therapy in the framework of MTE, including autologous bone marrow-derived or cardiac progenitors, as well as embryonic or induced pluripotent stem cells, each having their particular advantages and disadvantages. The book covers a complete range of biomaterials, examining different aspects of their application in MTE, such as biocompatibility with cardiac cells, mechanical capability and compatibility with the mechanical properties of the native myocardium as well as degradation behaviour in vivo and in vitro. Although a great deal of research is being carried out in the field, this book also addresses many questions that still remain unanswered and highlights those areas in which further research efforts are required. The book will also give an insight into clinical trials and possible novel cell sources for cell therapy in MTE.
Author : J. Miguel Oliveira
Publisher : Springer Nature
ISBN 13 : 3030365883
Total Pages : 176 pages
Book Rating : 4.0/5 (33 download)
Download or read book Biomaterials- and Microfluidics-Based Tissue Engineered 3D Models written by J. Miguel Oliveira and published by Springer Nature. This book was released on 2020-04-13 with total page 176 pages. Available in PDF, EPUB and Kindle. Book excerpt: This contributed volume reviews the latest advances on relevant 3D tissue engineered in vitro models of disease making use of biomaterials and microfluidics. The main focus of this book is on advanced biomaterials and microfluidics technologies that have been used in in vitro mimetic 3D models of human diseases and show great promise in revolutionizing personalized medicine. Readers will discover important topics involving biomaterials and microfluidics design, advanced processing techniques, and development and validation of organ- and body-on-a-chip models for bone, liver, and cancer research. An in depth discussion of microfabrication methods for microfluidics development is also provided. This work is edited by two truly multidisciplinary scientists and includes important contributions from well-known experts in their fields. The work is written for both early stage and experienced researchers, and well-established scientists enrolled in the fields of biomaterials, microfluidics, and tissue engineering, and is especially suited to those who wish to become acquainted with the principles and latest developments of in vitro models of diseases, such as professionals working in pharma, medicine, and engineering.
