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The Relationship Between Pathogenic And Nonpathogenic Strains Of Pseudomonas Syringae And Bacterial Brown Spot Disease In Beans
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Book Synopsis The Relationship Between Pathogenic and Nonpathogenic Strains of Pseudomonas Syringae and Bacterial Brown Spot Disease in Beans by : Rui Pereira Leite
Download or read book The Relationship Between Pathogenic and Nonpathogenic Strains of Pseudomonas Syringae and Bacterial Brown Spot Disease in Beans written by Rui Pereira Leite and published by . This book was released on 1986 with total page 250 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis The Relationship Between Pathogenic and Nonpathogenic Strains of Pseudomonas Syringae and Bacterial Brown Spot Disease in Beans by : R. P. Leite Junior
Download or read book The Relationship Between Pathogenic and Nonpathogenic Strains of Pseudomonas Syringae and Bacterial Brown Spot Disease in Beans written by R. P. Leite Junior and published by . This book was released on 1986 with total page 107 pages. Available in PDF, EPUB and Kindle. Book excerpt: Studies were conducted to determine the ability of nonpathogenic strains of Pseudomonas syringae pv. syringae to colonize the snap bean phylloplane. The effect of colonization by nonpathogenic P. syringae pv. syringae, was examined under field conditons. Strains of P. syringae nonpathogenic to beans originally obtained from bean leaf surface were introduced into field plots by seed treatment before planting and/or by spray application. Bacterial population sizes on bean leaflets and pods were estimated by dilution plating from bulked samples. The nonpathogenic strains of P. syringae colonized bean leaflets and pods, and reached populations for 10 [elevado a 3] to 10 [elevado a 5] CFU/ leaflet or pod. However, dry weather was apparently unfavorable for the establishment of the nonpathogenic strains on bean plants. The population sizes of the nonpathogenic strains remined below 10 [elevado a 3] CFU/ leaflet under these conditions. The presence of high epiphytic populations of P. syringae-like bacteria (PSLB) (10 [elevado a 6] CFU/leaflet) on bean plants, apparently prevented the establishment of large epiphytic populations (10 [elevado a 3] CFU/leaflet) of the nonpathogenic strains of P. syringae. The nonpathogenic strains failed to reduce the occurrence of bacterial brown spot, in these cases. Decreased number of naturally occurring were observed on tretments sprayed with PSLB nonpathogenic strains were applied prior to establishment of high populations of PSLB. Significant reductions (p
Book Synopsis The Etiology and Epidemiology of Bacterial Brown Spot of Bean Caused by Pseudomonas Syringae Pathovar Syringae by : Daniel Edwin Legard
Download or read book The Etiology and Epidemiology of Bacterial Brown Spot of Bean Caused by Pseudomonas Syringae Pathovar Syringae written by Daniel Edwin Legard and published by . This book was released on 1991 with total page 238 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Biological and Molecular Characterization of Wild Type Parental and Nonpathogenic Mutant Strains of Pseudomonas Syringae Pathovars Phaseolicola and Syringae by : Gregory Bertoni
Download or read book Biological and Molecular Characterization of Wild Type Parental and Nonpathogenic Mutant Strains of Pseudomonas Syringae Pathovars Phaseolicola and Syringae written by Gregory Bertoni and published by . This book was released on 1987 with total page 238 pages. Available in PDF, EPUB and Kindle. Book excerpt: The in planta growth of three wild type strains of phytopathogenic bacteria and three nonpathogenic mutant strains was studied to determine if mutations eliminating the ability of the mutant strains to cause disease had affected their growth in leaf tissue. The mutant strains were obtained by Tn5 mutagenesis of Pseudomonas svringae pv. phaseolicola strain PP7010 and P. svringae pv. svringae strain PS9020, organisms which respectively cause halo blight of bean and bacterial brown spot of bean. Through cloning of Tn5-containing genomic DNA, bacterial conjugation, auxanography, and genetic marker exchange experiments, these studies demonstrated that the Tn5 mutation in strain PP7014 of P. svringae pv. phaseolicola had created an auxotrophic requirement for uracil and had caused an inability to produce typical necrotic and chlorotic disease symptoms in bean leaves. A simple and reproducible bioassay was developed to monitor bacterial growth in leaf tissue. Using this bioassay, mutant PP7014 was shown to be incapable of growth in planta, presumably due to its inability to obtain uracil for growth in the leaf. However, a similar Tn5 arginine auxotroph, PP7510, was capable of multiplication in bean leaves, demonstrating that prototrophy of P. svringae pv. phaseolicola is not a prerequisite for pathogenicity. Growth studies with pathogenic wild type P. syringae pv. syringae strains J900 and PS9020 revealed a correlation between growth in planta and the severity of symptom expression. Altered growth patterns and symptom expression were shown for two nonpathogenic strains derived by Tn5. mutagenesis of PS9020. Mutant strain PS9021 was incapable of in planta growth and disease symptom expression. Mutant strain PS9024, although initially capable of rapid multiplication in bean leaves, was unable to maintain the high population levels and produce the characteristic symptoms of the parental wild type strain. Further growth studies were conducted to investigate the complementation of the Tn5 mutation in PS9021 by cosmids containing wild type PS9020 DNA homologous to the mutated region. Although stable throughout multiple rounds of bacterial replication, the cosmids could partially, but not completely, restore the wild type in planta growth and symptom expression pattern to PS9021.
Book Synopsis Cropping Pattern, Epiphytic Population of Pseudomonas Syringae, and Incidence of Brown Spot on Snap Beans (Phaseolus Vulgaris L.) in Central Wisconsin by : Julianne Lindemann
Download or read book Cropping Pattern, Epiphytic Population of Pseudomonas Syringae, and Incidence of Brown Spot on Snap Beans (Phaseolus Vulgaris L.) in Central Wisconsin written by Julianne Lindemann and published by . This book was released on 1981 with total page 338 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Identification and Inheritance of Resistance to Bacterial Brown Spot (Pseudomonas Syringae) in Phaseolus Vulgaris by : Steven Hales Antonius
Download or read book Identification and Inheritance of Resistance to Bacterial Brown Spot (Pseudomonas Syringae) in Phaseolus Vulgaris written by Steven Hales Antonius and published by . This book was released on 1982 with total page 702 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Bacterial Blights of Common Bean by : Michael Lennon O'Leary
Download or read book Bacterial Blights of Common Bean written by Michael Lennon O'Leary and published by . This book was released on 2018 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: Common bean is a genetically diverse crop that is a staple food in many regions of the world. However, common bean is affected by numerous diseases that can negatively impact production. There are three major bacterial diseases of common bean: halo blight, caused by Pseudomonas syringae pv. phaseolicola (Psp), bacterial brown spot, caused by P. syringae pv. syringae (Pss), and common bacterial blight, caused by Xanthomonas axonopodis pv. phaseoli (Xap) and X. fuscans subsp. fuscans (Xff). Disease symptoms caused by Psp and Pss can be difficult to distinguish in certain common bean genotypes and under certain environmental conditions. Precise pathogen identification is important in terms of potential yield loss and the need for disease management. Symptoms with characteristics of both halo blight and bacterial brown spot were observed on a newly developed cultivar of dark red kidney beans in Wisconsin, and pseudomonads were recovered from all samples. To determine the etiology of this disease, a method of identification was developed that involved 1) PCR with primers specific for toxin genes utilized by Psp and Pss, 2) the ability to induce a hypersensitivity response in tobacco leaves, and 3) symptoms induced on common bean. When applied to the unusual disease phenotype on this dark red kidney variety most of these were atypical symptoms of halo blight. This method was then successfully applied to identify bacterial brown spot in disease samples from Oregon, and halo blight and bacterial brown spot in disease samples from Turkey. Xap and Xff strains induce identical symptoms on common bean but are genetically distinct species. Strains of Xap and Xff can be differentiated in culture by the production of a brown diffusible pigment by Xff strains. However, some Xap strains, particularly those that originate from East Africa, are more genetically related to Xff strains than to the globally predominant Xap strains, and show pathogenic variability on certain cultivars, e.g., Andean versus Middle American. To better understand the genetic diversity among Xap and Xff strains, the complete genome sequences of three globally predominant Xap strains, two East African Xap strains, three Xff strains, and one Xanthomonas spp. strain that is associated with common bean but not pathogenic were determined using long-read sequencing technology. Whole-genome alignment revealed differences in chromosome organization between Xap strains, East African Xap strains, and Xff strains; additionally, mobile element content is variable among these strains. Conserved genetic factors, e.g. conserved plasmids and type III secreted effector proteins, some of which may have been horizontally acquired by Xap and Xff strains, were identified. The brown diffusible pigment produced by Xff strains is the result of an oxidation product of homogentisic acid, which is referred to as pyomelanin. In the tyrosine catabolism pathway, homogentisic acid is produced by 4-hydroxyphenylpyruvate dioxygenase (HPPD) and converted to 4-maleylacetoacetate by homogentisate dioxygenase (HmgA). The complete genome sequences of Xap and Xff strains revealed that Xff strains have a conserved mutation in the hmgA gene, whereas Xap strains have an intact hmgA gene. HPPD deletion mutants in an Xff strain resulted in loss of pyomelanin (brown pigment) production, whereas deletion of hmgA in two Xap strains resulted in brown pigment production, making Xap colonies resemble those of wild-type Xff strains. Additionally, complementing a wild-type Xff strain with a functional hmgA gene eliminated visible pyomelanin accumulation. Finally, virulence of a Xff HPPD and a Xap hmgA deletion mutant was assessed on twelve bean cultivars, including both Andean and Middle American gene pools, with different levels of disease resistance to common bacterial blight. There was no significant differences in virulence between brown pigment mutants and wild-type strains.
Book Synopsis Studies of the Spatial Patterns of Bacterial Brown Spot of Snap Beans by : Brian Dean Hudelson
Download or read book Studies of the Spatial Patterns of Bacterial Brown Spot of Snap Beans written by Brian Dean Hudelson and published by . This book was released on 1990 with total page 342 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Bacterial Brown Spot of Beans written by and published by . This book was released on 2014 with total page 1 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Sugar Availability as a Limit to Growth of Pathogenic and Non-pathogenic Bacteria in Bean Leaves by : Jessica Louise Ray
Download or read book Sugar Availability as a Limit to Growth of Pathogenic and Non-pathogenic Bacteria in Bean Leaves written by Jessica Louise Ray and published by . This book was released on 2002 with total page 142 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Pseudomonas syringae Pathovars and Related Pathogens - Identification, Epidemiology and Genomics by : M’Barek Fatmi
Download or read book Pseudomonas syringae Pathovars and Related Pathogens - Identification, Epidemiology and Genomics written by M’Barek Fatmi and published by Springer. This book was released on 2008-08-14 with total page 433 pages. Available in PDF, EPUB and Kindle. Book excerpt: The Conference on Pseudomonas syringae which started in 1973 as an informal meeting of a group of scientists working on these bacteria in Angers, France, has become more and more important with time. Many meetings have been held since then: 1984, 1987, 1991, 1995, and 2002 in Cape Sounion, Greece; Lisbon, Portugal; Florence, Italy; Berlin, Germany; and Maratea, Italy; respectively. This Conference is considered as the most important scientific forum in which recent advances in different research aspects on Pseudomonas syringae, a plant pathogenic bacterial species that includes a high number of pathogens (referred as pathovars) and Related Pathogens such as Acidovorax, Burkholderia, Ralstonia, affecting several economically important crops. The proceedings resulting from these meetings are considered as valuable sources of information related to this group of pathogens. The interest in organising this conference regularly is reflected by the attendance of more than 80 scientists from 20 countries worldwide, who participated at the 7th International Conference on Pseudomonas syringae pathovars and related pat- gens organized by the Institut Agronomique et Vétérinaire Hassan II in Agadir, Morocco, from 13th to 16th November 2006.
Book Synopsis Pseudomonas Syringae Pathovars and Related Pathogens by : K. Rudolph
Download or read book Pseudomonas Syringae Pathovars and Related Pathogens written by K. Rudolph and published by Springer Science & Business Media. This book was released on 1997-06-30 with total page 714 pages. Available in PDF, EPUB and Kindle. Book excerpt: During the last decade, research on Pseudomonas syringae pathovars and related pathogens has progressed rapidly, opening up many new avenues. The application of molecular genetics has provided new insights into determinants of pathogenicity and virulence. Progress has also been made in elucidating the chemical structures and modes of action of phytotoxins from Pseudomonas syringae; by establishing novel strategies for disease control; in biotechnological applications; by studying the resistant reaction of the plant with a combined biochemical and genetic approach; and in the development of new detection and identification methodologies as tools in epidemiological studies. With such rapid advances it becomes more and more difficult to keep abreast of the developments and concepts within disciplines, all involving research on pathovars of P. syringae. In an attempt to provide a balanced overview, recent developments in these rapidly expanding fields have been critically reviewed at the beginning of each chapter by internationally renowned experts. Our comprehensive coverage has been made possible because all the contributors to this volume presented their latest findings at the `5th International Conference on Pseudomonas syringae Pathovars and Related Pathogens' in Berlin, September 3-8, 1995. In this way, it was possible to bring together contributions from a wide range of fields including phytopathology, genetics, bacteriology, plant breeding, plant protection, and taxonomy. This book is not intended simply as a record of the proceedings of the Berlin Conference, but as an extension of recent findings and hypotheses put forward at the meeting. All papers published in this volume have been reviewed by the Editors.
Book Synopsis Annual Review of Phytopathology by : R. J. Cook
Download or read book Annual Review of Phytopathology written by R. J. Cook and published by Annual Reviews. This book was released on 1990 with total page 522 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Crop Improvement Utilizing Biotechnology by : Rup Lal
Download or read book Crop Improvement Utilizing Biotechnology written by Rup Lal and published by CRC Press. This book was released on 2019-07-23 with total page 364 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book discusses; somaclonal variation in crop improvement, the role of tissue culture in rapid clonal propagation and production of pathogen-free plant, protoplasts in crop improvement, cell selection and long-term high-frequency regeneration of cereals and legumes, agrobacteria-mediated gene transformation and vectors for gene cloning in plants, and plant frost injury and its management.
Download or read book Pseudomonas written by Enrica Galli and published by Wiley-Blackwell. This book was released on 1992 with total page 466 pages. Available in PDF, EPUB and Kindle. Book excerpt: Based on the proceedings of a symposium held under the auspices of the Federation of European Microbiological Societies, June 1991, Trieste, Italy, devoted to the ubiquitous bacteria with promising potential as a biological ally in agriculture, the chemical industry, and environmental bioremediation. Contributors discuss biological properties as well as biotechnological uses. Annotation copyrighted by Book News, Inc., Portland, OR
Book Synopsis UCLA Symposia on Molecular & Cellular Biology by :
Download or read book UCLA Symposia on Molecular & Cellular Biology written by and published by . This book was released on 1986 with total page 280 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Annual Review of Phytopathology by : Kenneth P. Baker
Download or read book Annual Review of Phytopathology written by Kenneth P. Baker and published by . This book was released on 1974 with total page 516 pages. Available in PDF, EPUB and Kindle. Book excerpt:
