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The Interaction Of Base Analog Subsituted Lambda Pr Promoters With E Coli Rna Polymerase
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Book Synopsis The Interaction of Base-analog Subsituted Lambda PR̳ Promoters with E. Coli RNA Polymerase by : John Woods Dubendorff
Download or read book The Interaction of Base-analog Subsituted Lambda PR̳ Promoters with E. Coli RNA Polymerase written by John Woods Dubendorff and published by . This book was released on 1985 with total page 262 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis The Interaction of E. Coli RNA Polymerase with Lambda Phage PR Promoter by : Won-Chul Suh
Download or read book The Interaction of E. Coli RNA Polymerase with Lambda Phage PR Promoter written by Won-Chul Suh and published by . This book was released on 1993 with total page 430 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis The Interaction of Escherichia Coli RNA Polymerase with Phage [lambda] Promoters by : Jung-Hye Roe
Download or read book The Interaction of Escherichia Coli RNA Polymerase with Phage [lambda] Promoters written by Jung-Hye Roe and published by . This book was released on 1984 with total page 422 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Interaction of E.coli RNA Polymerase with Phage [lambda] Promoters by : Elizabeth Margaret Owens
Download or read book Interaction of E.coli RNA Polymerase with Phage [lambda] Promoters written by Elizabeth Margaret Owens and published by . This book was released on 1979 with total page 172 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Kinetic Investigation of the Molecular Processes Involved in the Mechanism of Open Complex Formation Between E. Coli RNA Polymerase and the [lambda]Pr Promoter by : Wayne Kontur
Download or read book Kinetic Investigation of the Molecular Processes Involved in the Mechanism of Open Complex Formation Between E. Coli RNA Polymerase and the [lambda]Pr Promoter written by Wayne Kontur and published by . This book was released on 2006 with total page 200 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Studies on the Interaction of E. Coli RNA Polymerase with Lactose Promoter DNA by : Donald Duane Lorimer
Download or read book Studies on the Interaction of E. Coli RNA Polymerase with Lactose Promoter DNA written by Donald Duane Lorimer and published by . This book was released on 1989 with total page 260 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Kinetics and Mechanism of Open Complex Formation, Stabilization, and Initiation by E. Coli RNA Polymerase at [lambda]PR and T7A1 Promoters by : Hao-Che Wang
Download or read book Kinetics and Mechanism of Open Complex Formation, Stabilization, and Initiation by E. Coli RNA Polymerase at [lambda]PR and T7A1 Promoters written by Hao-Che Wang and published by . This book was released on 2022 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: The flow of genetic information in cells starts with transcription by the enzyme RNA polymerase (RNAP) of DNA information to synthesize a wide variety of structural and functional RNAs which collectively participate in most cellular processes. Transcription is therefore a highly regulated step in gene expression. Research discussed here focuses on transcription initiation, the earliest phase of transcription, and on questions of how differences in promoter DNA sequences and length affect initiation thermodynamics and kinetics. During transcription initiation, RNAP binds to and unwinds the transcription start site region of promoter DNA, opening 13 bp to form an open complex (OC). Chapter 2 reports a detailed kinetic-mechanistic comparison of OC formation for the model λPR and T7A1 promoters, and Chapter 3 does the same for OC dissociation kinetics and mechanisms. For OC formation, kinetics of 2-amino purine (-4) fluorescence, Cy3 (-100) - Cy5 (+14) FRET, and single-dye PIFE were determined and compared with filter binding kinetics of formation of long-lived promoter complexes. A range of temperatures and of glycine betaine concentrations was examined. From these comparisons, we conclude that early steps in the mechanism of OC formation are similar for the two promoters while later steps including DNA opening differ significantly. Our data are consistent with previous reports that opening of the T7A1 bubble occurs in two adjacent kinetic steps vs. one step for λPR. After the DNA opening transition state, the kinetics and mechanisms of OC formation at these two promoters become similar again. In Chapter 3, we investigated the roles of clamp closing and of allosteric effects of discriminator and upstream interactions on downstream elements in stabilizing E. coli RNA polymerase- λPR promoter open complexes (OC). Our approach is to determine dissociation rate constants for the stable OC (kd) and for the unstable I2 intermediate OC (k-2) at λPR and T7A1 promoters and promoter variants with exchanged discriminators, core promoters or UP elements by filter binding assays. Values of kd are also determined for downstream-truncated promoters (at +12, +6) and for a jaw deletion RNAP variant. Analysis yields free energy changes 8́6G3^o for the conversion of unstable I2 to a stable OC, and the large contribution to 8́6G3^o for all promoters, from clamp closing on the promoter from +6 upstream, as well as the significant contributions to 8́6G3^o for promoters with the λPR discriminator from downstream interactions with the Îø lobe and Îø' jaw that are allosterically controlled by interactions of the discriminator with ϳ1.2 and the Îø gate loop. Lastly, in chapter 4, we further test how promoters with different OC stability affect the escaping of the RNAP from promoter. Preliminary results indicate the OC lifetime mainly alters the rate for converting the OC into initial complex (IC) for NTP incorporations. Taken together, our studies provide a more complete understanding of how promoter sequence and length influences different phases of transcription initiation.
Download or read book Cumulated Index Medicus written by and published by . This book was released on 1987 with total page 1220 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Chemica Scripta written by and published by . This book was released on 1986 with total page 688 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Dissertation Abstracts International by :
Download or read book Dissertation Abstracts International written by and published by . This book was released on 1986 with total page 626 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Proceedings of the Robert A. Welch Foundation Conferences on Chemical Research by :
Download or read book Proceedings of the Robert A. Welch Foundation Conferences on Chemical Research written by and published by . This book was released on 1985 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Comprehensive Dissertation Index by :
Download or read book Comprehensive Dissertation Index written by and published by . This book was released on 1989 with total page 754 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Chemical Synthesis in Molecular Biology by : Helmut Blöcker
Download or read book Chemical Synthesis in Molecular Biology written by Helmut Blöcker and published by . This book was released on 1987 with total page 240 pages. Available in PDF, EPUB and Kindle. Book excerpt: Protein-DNA recognition: The ineraction of lac. cl and E.coli RNA plymerase with Operators and promotors 1. Rapide solid-phase phosphotriester synthesis of DNA fragments on controlled pore glass and appçication to the preparation of a gene for somatomedin C 13. Cleavage of phosphorothioate-containing oligonucleotides and DNA by restriction endonucleases. Regio-and stereospecific synthesis of 7-Deazapurine 2 - Deoxyribonucleosides and incorporation of nucleooside isosteres into oligonucleotides. Repair-resistant nucleoside analogues in oligonucleotide-directed mutagenesis. Total synthesis of yeast alanyl tRNA. Synthesis of 5-Azacytosine 2'-Deoxyribonucleosides, proprerties and biological activity. Synthesis and characterization of a set of four dodecadeoxyribonucleoside Undecaphospates containing 0 - Methylguanine opposite ademine, cytosine, Guanine, and thymine. Chemical synthesis and biological applications of oligodeoxynucleotides containig carcinogen-DNA Adducts: 06-Methylguanine. Biotinylated ologonucleotide hybridization probes: Progress towards a Non-radioactive method for the deiagnosis of human genetic diseases.
Book Synopsis American Doctoral Dissertations by :
Download or read book American Doctoral Dissertations written by and published by . This book was released on 1985 with total page 696 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Index Medicus written by and published by . This book was released on 2004 with total page 2068 pages. Available in PDF, EPUB and Kindle. Book excerpt: Vols. for 1963- include as pt. 2 of the Jan. issue: Medical subject headings.
Download or read book Nucleic Acids Abstracts written by and published by . This book was released on 1996 with total page 1030 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Biological & Agricultural Index by :
Download or read book Biological & Agricultural Index written by and published by . This book was released on 1993 with total page 2728 pages. Available in PDF, EPUB and Kindle. Book excerpt: