Tag Based Next Generation Sequencing

Download Tag Based Next Generation Sequencing full books in PDF, epub, and Kindle. Read online Tag Based Next Generation Sequencing ebook anywhere anytime directly on your device. Fast Download speed and no annoying ads. We cannot guarantee that every ebooks is available!

Tag-based Next Generation Sequencing

Author : Matthias Harbers
Publisher : John Wiley & Sons
ISBN 13 : 3527644571
Total Pages : 609 pages
Book Rating : 4.5/5 (276 download)

DOWNLOAD NOW!

Book Synopsis Tag-based Next Generation Sequencing by : Matthias Harbers

Download or read book Tag-based Next Generation Sequencing written by Matthias Harbers and published by John Wiley & Sons. This book was released on 2011-10-21 with total page 609 pages. Available in PDF, EPUB and Kindle. Book excerpt: Tag-based approaches were originally designed to increase the throughput of capillary sequencing, where concatemers of short sequences were first used in expression profiling. New Next Generation Sequencing methods largely extended the use of tag-based approaches as the tag lengths perfectly match with the short read length of highly parallel sequencing reactions. Tag-based approaches will maintain their important role in life and biomedical science, because longer read lengths are often not required to obtain meaningful data for many applications. Whereas genome re-sequencing and de novo sequencing will benefit from ever more powerful sequencing methods, analytical applications can be performed by tag-based approaches, where the focus shifts from 'sequencing power' to better means of data analysis and visualization for common users. Today Next Generation Sequence data require powerful bioinformatics expertise that has to be converted into easy-to-use data analysis tools. The book's intention is to give an overview on recently developed tag-based approaches along with means of their data analysis together with introductions to Next-Generation Sequencing Methods, protocols and user guides to be an entry for scientists to tag-based approaches for Next Generation Sequencing.

Next Generation Sequencing

Author : Jerzy Kulski
Publisher : BoD – Books on Demand
ISBN 13 : 9535122401
Total Pages : 466 pages
Book Rating : 4.5/5 (351 download)

DOWNLOAD NOW!

Book Synopsis Next Generation Sequencing by : Jerzy Kulski

Download or read book Next Generation Sequencing written by Jerzy Kulski and published by BoD – Books on Demand. This book was released on 2016-01-14 with total page 466 pages. Available in PDF, EPUB and Kindle. Book excerpt: Next generation sequencing (NGS) has surpassed the traditional Sanger sequencing method to become the main choice for large-scale, genome-wide sequencing studies with ultra-high-throughput production and a huge reduction in costs. The NGS technologies have had enormous impact on the studies of structural and functional genomics in all the life sciences. In this book, Next Generation Sequencing Advances, Applications and Challenges, the sixteen chapters written by experts cover various aspects of NGS including genomics, transcriptomics and methylomics, the sequencing platforms, and the bioinformatics challenges in processing and analysing huge amounts of sequencing data. Following an overview of the evolution of NGS in the brave new world of omics, the book examines the advances and challenges of NGS applications in basic and applied research on microorganisms, agricultural plants and humans. This book is of value to all who are interested in DNA sequencing and bioinformatics across all fields of the life sciences.

Tag-based Next Generation Sequencing

Author : Matthias Harbers
Publisher : John Wiley & Sons
ISBN 13 : 352732819X
Total Pages : 609 pages
Book Rating : 4.5/5 (273 download)

DOWNLOAD NOW!

Book Synopsis Tag-based Next Generation Sequencing by : Matthias Harbers

Download or read book Tag-based Next Generation Sequencing written by Matthias Harbers and published by John Wiley & Sons. This book was released on 2012-02-13 with total page 609 pages. Available in PDF, EPUB and Kindle. Book excerpt: Tag-based approaches were originally designed to increase the throughput of capillary sequencing, where concatemers of short sequences were first used in expression profiling. New Next Generation Sequencing methods largely extended the use of tag-based approaches as the tag lengths perfectly match with the short read length of highly parallel sequencing reactions. Tag-based approaches will maintain their important role in life and biomedical science, because longer read lengths are often not required to obtain meaningful data for many applications. Whereas genome re-sequencing and de novo sequencing will benefit from ever more powerful sequencing methods, analytical applications can be performed by tag-based approaches, where the focus shifts from 'sequencing power' to better means of data analysis and visualization for common users. Today Next Generation Sequence data require powerful bioinformatics expertise that has to be converted into easy-to-use data analysis tools. The book's intention is to give an overview on recently developed tag-based approaches along with means of their data analysis together with introductions to Next-Generation Sequencing Methods, protocols and user guides to be an entry for scientists to tag-based approaches for Next Generation Sequencing.

Next-Generation Sequencing and Sequence Data Analysis

Author : Kuo Ping Chiu
Publisher :
ISBN 13 : 9781681080932
Total Pages : 162 pages
Book Rating : 4.0/5 (89 download)

DOWNLOAD NOW!

Book Synopsis Next-Generation Sequencing and Sequence Data Analysis by : Kuo Ping Chiu

Download or read book Next-Generation Sequencing and Sequence Data Analysis written by Kuo Ping Chiu and published by . This book was released on 2018-01-22 with total page 162 pages. Available in PDF, EPUB and Kindle. Book excerpt: Nucleic acid sequencing techniques have enabled researchers to determine the exact order of base pairs - and by extension, the information present - in the genome of living organisms. Consequently, our understanding of this information and its link to genetic expression at molecular and cellular levels has lead to rapid advances in biology, genetics, biotechnology and medicine. Next-Generation Sequencing and Sequence Data Analysis is a brief primer on DNA sequencing techniques and methods used to analyze sequence data. Readers will learn about recent concepts and methods in genomics such as sequence library preparation, cluster generation for PCR technologies, PED sequencing, genome assembly, exome sequencing, transcriptomics and more. This book serves as a textbook for students undertaking courses in bioinformatics and laboratory methods in applied biology. General readers interested in learning about DNA sequencing techniques may also benefit from the simple format of information presented in the book.

Algorithms for Next-Generation Sequencing Data

Author : Mourad Elloumi
Publisher : Springer
ISBN 13 : 3319598260
Total Pages : 356 pages
Book Rating : 4.3/5 (195 download)

DOWNLOAD NOW!

Book Synopsis Algorithms for Next-Generation Sequencing Data by : Mourad Elloumi

Download or read book Algorithms for Next-Generation Sequencing Data written by Mourad Elloumi and published by Springer. This book was released on 2017-09-18 with total page 356 pages. Available in PDF, EPUB and Kindle. Book excerpt: The 14 contributed chapters in this book survey the most recent developments in high-performance algorithms for NGS data, offering fundamental insights and technical information specifically on indexing, compression and storage; error correction; alignment; and assembly. The book will be of value to researchers, practitioners and students engaged with bioinformatics, computer science, mathematics, statistics and life sciences.

Clinical Applications for Next-Generation Sequencing

Author : Urszula Demkow
Publisher : Academic Press
ISBN 13 : 0128018410
Total Pages : 336 pages
Book Rating : 4.1/5 (28 download)

DOWNLOAD NOW!

Book Synopsis Clinical Applications for Next-Generation Sequencing by : Urszula Demkow

Download or read book Clinical Applications for Next-Generation Sequencing written by Urszula Demkow and published by Academic Press. This book was released on 2015-09-10 with total page 336 pages. Available in PDF, EPUB and Kindle. Book excerpt: Clinical Applications for Next Generation Sequencing provides readers with an outstanding postgraduate resource to learn about the translational use of NGS in clinical environments. Rooted in both medical genetics and clinical medicine, the book fills the gap between state-of-the-art technology and evidence-based practice, providing an educational opportunity for users to advance patient care by transferring NGS to the needs of real-world patients. The book builds an interface between genetic laboratory staff and clinical health workers to not only improve communication, but also strengthen cooperation. Users will find valuable tactics they can use to build a systematic framework for understanding the role of NGS testing in both common and rare diseases and conditions, from prenatal care, like chromosomal abnormalities, up to advanced age problems like dementia. Fills the gap between state-of-the-art technology and evidence-based practice Provides an educational opportunity which advances patient care through the transfer of NGS to real-world patient assessment Promotes a practical tool that clinicians can apply directly to patient care Includes a systematic framework for understanding the role of NGS testing in many common and rare diseases Presents evidence regarding the important role of NGS in current diagnostic strategies

Next Generation Sequencing

Author : Lee-Jun C. Wong
Publisher : Springer Science & Business Media
ISBN 13 : 1461470013
Total Pages : 302 pages
Book Rating : 4.4/5 (614 download)

DOWNLOAD NOW!

Book Synopsis Next Generation Sequencing by : Lee-Jun C. Wong

Download or read book Next Generation Sequencing written by Lee-Jun C. Wong and published by Springer Science & Business Media. This book was released on 2013-05-31 with total page 302 pages. Available in PDF, EPUB and Kindle. Book excerpt: In recent years, owing to the fast development of a variety of sequencing technologies in the post human genome project era, sequencing analysis of a group of target genes, entire protein coding regions of the human genome, and the whole human genome has become a reality. Next Generation Sequencing (NGS) or Massively Parallel Sequencing (MPS) technologies offers a way to screen for mutations in many different genes in a cost and time efficient manner by deep coverage of the target sequences. This novel technology has now been applied to clinical diagnosis of Mendelian disorders of well characterized or undefined diseases, discovery of new disease genes, noninvasive prenatal diagnosis using maternal blood, and population based carrier testing of severe autosomal recessive disorders. This book covers topics of these applications, including potential limitations and expanded application in the future.

Next-Generation Sequencing Data Analysis

Author : Xinkun Wang
Publisher : CRC Press
ISBN 13 : 1482217899
Total Pages : 252 pages
Book Rating : 4.4/5 (822 download)

DOWNLOAD NOW!

Book Synopsis Next-Generation Sequencing Data Analysis by : Xinkun Wang

Download or read book Next-Generation Sequencing Data Analysis written by Xinkun Wang and published by CRC Press. This book was released on 2016-04-06 with total page 252 pages. Available in PDF, EPUB and Kindle. Book excerpt: A Practical Guide to the Highly Dynamic Area of Massively Parallel SequencingThe development of genome and transcriptome sequencing technologies has led to a paradigm shift in life science research and disease diagnosis and prevention. Scientists are now able to see how human diseases and phenotypic changes are connected to DNA mutation, polymorphi

Algorithms for Next-Generation Sequencing

Author : Wing-Kin Sung
Publisher : CRC Press
ISBN 13 : 1466565519
Total Pages : 362 pages
Book Rating : 4.4/5 (665 download)

DOWNLOAD NOW!

Book Synopsis Algorithms for Next-Generation Sequencing by : Wing-Kin Sung

Download or read book Algorithms for Next-Generation Sequencing written by Wing-Kin Sung and published by CRC Press. This book was released on 2017-05-18 with total page 362 pages. Available in PDF, EPUB and Kindle. Book excerpt: Advances in sequencing technology have allowed scientists to study the human genome in greater depth and on a larger scale than ever before – as many as hundreds of millions of short reads in the course of a few days. But what are the best ways to deal with this flood of data? Algorithms for Next-Generation Sequencing is an invaluable tool for students and researchers in bioinformatics and computational biology, biologists seeking to process and manage the data generated by next-generation sequencing, and as a textbook or a self-study resource. In addition to offering an in-depth description of the algorithms for processing sequencing data, it also presents useful case studies describing the applications of this technology.

Next-Generation Genome Sequencing

Author : Michal Janitz
Publisher : John Wiley & Sons
ISBN 13 : 3527644733
Total Pages : 281 pages
Book Rating : 4.5/5 (276 download)

DOWNLOAD NOW!

Book Synopsis Next-Generation Genome Sequencing by : Michal Janitz

Download or read book Next-Generation Genome Sequencing written by Michal Janitz and published by John Wiley & Sons. This book was released on 2011-08-24 with total page 281 pages. Available in PDF, EPUB and Kindle. Book excerpt: Written by leading experts from industry and academia, this first single comprehensive resource addresses recent developments in next generation DNA sequencing technology and their impact on genome research, drug discovery and health care. As such, it presents a detailed comparative analysis of commercially available platforms as well as insights into alternative, emerging sequencing techniques. In addition, the book not only covers the principles of DNA sequencing techniques but also social, ethical and commercial aspects, the concept of personalized medicine and a five-year perspective of DNA sequencing.

Next Generation Sequencing and Sequence Assembly

Author : Ali Masoudi-Nejad
Publisher : Springer Science & Business Media
ISBN 13 : 1461477263
Total Pages : 92 pages
Book Rating : 4.4/5 (614 download)

DOWNLOAD NOW!

Book Synopsis Next Generation Sequencing and Sequence Assembly by : Ali Masoudi-Nejad

Download or read book Next Generation Sequencing and Sequence Assembly written by Ali Masoudi-Nejad and published by Springer Science & Business Media. This book was released on 2013-07-09 with total page 92 pages. Available in PDF, EPUB and Kindle. Book excerpt: The goal of this book is to introduce the biological and technical aspects of next generation sequencing methods, as well as algorithms to assemble these sequences into whole genomes. The book is organized into two parts; part 1 introduces NGS methods and part 2 reviews assembly algorithms and gives a good insight to these methods for readers new to the field. Gathering information, about sequencing and assembly methods together, helps both biologists and computer scientists to get a clear idea about the field. Chapters will include information about new sequencing technologies such as ChIp-seq, ChIp-chip, and De Novo sequence assembly.

Next Generation Sequencing in Forensic Science

Author : Kelly M. Elkins
Publisher : CRC Press
ISBN 13 : 1000449254
Total Pages : 176 pages
Book Rating : 4.0/5 (4 download)

DOWNLOAD NOW!

Book Synopsis Next Generation Sequencing in Forensic Science by : Kelly M. Elkins

Download or read book Next Generation Sequencing in Forensic Science written by Kelly M. Elkins and published by CRC Press. This book was released on 2021-09-15 with total page 176 pages. Available in PDF, EPUB and Kindle. Book excerpt: Next Generation Sequencing in Forensic Science: A Primer addresses next generation sequencing (NGS) specific to its application to forensic science. The first part of the book offers a history of human identity approaches, including VNTR, RFLP, STR, and SNP DNA typing. It discusses the history of sequencing for human DNA typing, including Sanger sequencing, SNaPshot, pyrosequencing, and principles of next generation sequencing. The chapters present an overview of the forensically focused AmpliSeq, ForenSeq, Precision ID, PowerSeq, and QIAseq panels for human DNA typing using autosomal, Y and X chromosome STRs and SNPs using the MiSeq FGx and Ion Torrent System. The authors outline the steps included in DNA extraction and DNA quantitation that are performed prior to preparing libraries with the NGS kits. The second half of the book details the implementation of ForenSeq and Precision ID to amplify and tag targets to create the library, enrich targets to attach indexes and adaptors, perform library purification and normalization, pool the libraries, and load samples to the cartridge to perform the sequencing on the instrument. Coverage addresses the operation of the MiSeq FGx and Ion Chef, including creating a sample list, executing wash steps, performing NGS, understanding the run feedback files from the instrument, and troubleshooting. ForenSeq and Precision ID panel data analysis are explained, including how to analyze and interpret NGS data and output graphs and charts. The book concludes with mitochondrial DNA (mtDNA) sequencing and SNPs analysis, including the issue of heteroplasmy. The final chapters review forensic applications of microbial DNA, NGS in body fluid analysis, and challenges and considerations for future applications. FEATURES Focuses on human identification using traditional and NGS DNA typing methods targeting short tandem repeats (STRs) Applies the technology and its application to law enforcement investigations and identity and ancestry single nucleotide polymorphisms (SNPs) for investigational leads, mass disaster, and ancestry cases Presents the underlying principles of NGS in a clear, easy-to-understand format for practitioners and students studying DNA in forensic programs This is the first book to prepare practitioners to utilize and implement this new technology in their lab for casework, highlighting early applications of how NGS results have been used in court. The book can be utilized for upper-level undergraduate and graduate students taking courses focused on NGS concepts. Readers are expected to have a basic understanding of molecular and cellular biology and DNA typing.

Next Generation Sequencing Technologies and Challenges in Sequence Assembly

Author : Sara El-Metwally
Publisher : Springer Science & Business
ISBN 13 : 1493907158
Total Pages : 123 pages
Book Rating : 4.4/5 (939 download)

DOWNLOAD NOW!

Book Synopsis Next Generation Sequencing Technologies and Challenges in Sequence Assembly by : Sara El-Metwally

Download or read book Next Generation Sequencing Technologies and Challenges in Sequence Assembly written by Sara El-Metwally and published by Springer Science & Business. This book was released on 2014-04-19 with total page 123 pages. Available in PDF, EPUB and Kindle. Book excerpt: The introduction of Next Generation Sequencing (NGS) technologies resulted in a major transformation in the way scientists extract genetic information from biological systems, revealing limitless insight about the genome, transcriptome and epigenome of any species. However, with NGS, came its own challenges that require continuous development in the sequencing technologies and bioinformatics analysis of the resultant raw data and assembly of the full length genome and transcriptome. Such developments lead to outstanding improvements of the performance and coverage of sequencing and improved quality for the assembled sequences, nevertheless, challenges such as sequencing errors, expensive processing and memory usage for assembly and sequencer specific errors remains major challenges in the field. This book aims to provide brief overviews the NGS field with special focus on the challenges facing the NGS field, including information on different experimental platforms, assembly algorithms and software tools, assembly error correction approaches and the correlated challenges.

Computational Methods for Next Generation Sequencing Data Analysis

Author : Ion Mandoiu
Publisher : John Wiley & Sons
ISBN 13 : 1118169484
Total Pages : 460 pages
Book Rating : 4.1/5 (181 download)

DOWNLOAD NOW!

Book Synopsis Computational Methods for Next Generation Sequencing Data Analysis by : Ion Mandoiu

Download or read book Computational Methods for Next Generation Sequencing Data Analysis written by Ion Mandoiu and published by John Wiley & Sons. This book was released on 2016-10-03 with total page 460 pages. Available in PDF, EPUB and Kindle. Book excerpt: Introduces readers to core algorithmic techniques for next-generation sequencing (NGS) data analysis and discusses a wide range of computational techniques and applications This book provides an in-depth survey of some of the recent developments in NGS and discusses mathematical and computational challenges in various application areas of NGS technologies. The 18 chapters featured in this book have been authored by bioinformatics experts and represent the latest work in leading labs actively contributing to the fast-growing field of NGS. The book is divided into four parts: Part I focuses on computing and experimental infrastructure for NGS analysis, including chapters on cloud computing, modular pipelines for metabolic pathway reconstruction, pooling strategies for massive viral sequencing, and high-fidelity sequencing protocols. Part II concentrates on analysis of DNA sequencing data, covering the classic scaffolding problem, detection of genomic variants, including insertions and deletions, and analysis of DNA methylation sequencing data. Part III is devoted to analysis of RNA-seq data. This part discusses algorithms and compares software tools for transcriptome assembly along with methods for detection of alternative splicing and tools for transcriptome quantification and differential expression analysis. Part IV explores computational tools for NGS applications in microbiomics, including a discussion on error correction of NGS reads from viral populations, methods for viral quasispecies reconstruction, and a survey of state-of-the-art methods and future trends in microbiome analysis. Computational Methods for Next Generation Sequencing Data Analysis: Reviews computational techniques such as new combinatorial optimization methods, data structures, high performance computing, machine learning, and inference algorithms Discusses the mathematical and computational challenges in NGS technologies Covers NGS error correction, de novo genome transcriptome assembly, variant detection from NGS reads, and more This text is a reference for biomedical professionals interested in expanding their knowledge of computational techniques for NGS data analysis. The book is also useful for graduate and post-graduate students in bioinformatics.

Bioinformatics for High Throughput Sequencing

Author : Naiara Rodríguez-Ezpeleta
Publisher : Springer Science & Business Media
ISBN 13 : 1461407826
Total Pages : 258 pages
Book Rating : 4.4/5 (614 download)

DOWNLOAD NOW!

Book Synopsis Bioinformatics for High Throughput Sequencing by : Naiara Rodríguez-Ezpeleta

Download or read book Bioinformatics for High Throughput Sequencing written by Naiara Rodríguez-Ezpeleta and published by Springer Science & Business Media. This book was released on 2011-10-26 with total page 258 pages. Available in PDF, EPUB and Kindle. Book excerpt: Next generation sequencing is revolutionizing molecular biology. Owing to this new technology it is now possible to carry out a panoply of experiments at an unprecedented low cost and high speed. These go from sequencing whole genomes, transcriptomes and small non-coding RNAs to description of methylated regions, identification protein – DNA interaction sites and detection of structural variation. The generation of gigabases of sequence information for each of this huge bandwidth of applications in just a few days makes the development of bioinformatics applications for next generation sequencing data analysis as urgent as challenging.

Next Generation Sequencing Technologies in Medical Genetics

Author : C. Alexander Valencia
Publisher : Springer Science & Business Media
ISBN 13 : 1461490324
Total Pages : 101 pages
Book Rating : 4.4/5 (614 download)

DOWNLOAD NOW!

Book Synopsis Next Generation Sequencing Technologies in Medical Genetics by : C. Alexander Valencia

Download or read book Next Generation Sequencing Technologies in Medical Genetics written by C. Alexander Valencia and published by Springer Science & Business Media. This book was released on 2013-10-16 with total page 101 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book introduces readers to Next Generation Sequencing applications in medical genetics. The authors discuss the direct application of next-generation sequencing to medicine, specifically, laboratory medicine or molecular diagnostics. The first part of the book contains chapters on sanger sequencing, NGS technologies, targeted-amplification and capture, and exome sequencing. The second part of the book focuses on genetic disorders diagnoses by NGS, prenatal diagnosis, muscular dystrophies, mitochondrial disorders diagnosis, and challenges in molecular diagnosis. Recent developments and potential future trends in NGS sequencing applications are highlighted, as well.

Advancing Development of Synthetic Gene Regulators

Author : Anandhakumar Chandran
Publisher : Springer
ISBN 13 : 9811065470
Total Pages : 123 pages
Book Rating : 4.8/5 (11 download)

DOWNLOAD NOW!

Book Synopsis Advancing Development of Synthetic Gene Regulators by : Anandhakumar Chandran

Download or read book Advancing Development of Synthetic Gene Regulators written by Anandhakumar Chandran and published by Springer. This book was released on 2017-09-19 with total page 123 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book focuses on an “outside the box” notion by utilizing the powerful applications of next-generation sequencing (NGS) technologies in the interface of chemistry and biology. In personalized medicine, developing small molecules targeting a specific genomic sequence is an attractive goal. N-methylpyrrole (P)–N-methylimidazole (I) polyamides (PIPs) are a class of small molecule that can bind to the DNA minor groove. First, a cost-effective NGS (ion torrent platform)-based Bind-n-Seq was developed to identify the binding specificity of PIP conjugates in a randomized DNA library. Their biological influences rely primarily on selective DNA binding affinity, so it is important to analyze their genome-wide binding preferences. However, it is demanding to enrich specifically the small-molecule-bound DNA without chemical cross-linking or covalent binding in chromatinized genomes. Herein is described a method that was developed using high-throughput sequencing to map the differential binding sites and relative enriched regions of non-cross-linked SAHA-PIPs throughout the complex human genome. SAHA-PIPs binding motifs were identified and the genome-level mapping of SAHA-PIPs-enriched regions provided evidence for the differential activation of the gene network. A method using high-throughput sequencing to map the binding sites and relative enriched regions of alkylating PIP throughout the human genome was also developed. The genome-level mapping of alkylating the PIP-enriched region and the binding sites on the human genome identifies significant genomic targets of breast cancer. It is anticipated that this pioneering low-cost, high through-put investigation at the sequence-specific level will be helpful in understanding the binding specificity of various DNA-binding small molecules, which in turn will be beneficial for the development of small-molecule-based drugs targeting a genome-level sequence.