Author : Chinmay Nitin Afzulpurkar
Publisher :
ISBN 13 :
Total Pages : 102 pages
Book Rating : 4.:/5 (132 download)
Book Synopsis Study on Forming DNA-mediated Nanoparticle Conjugates by : Chinmay Nitin Afzulpurkar
Download or read book Study on Forming DNA-mediated Nanoparticle Conjugates written by Chinmay Nitin Afzulpurkar and published by . This book was released on 2022 with total page 102 pages. Available in PDF, EPUB and Kindle. Book excerpt: The objective of this thesis is to establish a rapid, robust, and reproducible procedure to controllably assemble DNA-functionalized Au nanoparticles (AuNPs) into satellite-shaped nanostructures using sequence-specific DNA hybridization. A target DNA strand with COVID-19sequence (t-DNA) is used as a bridge to connect a capture DNA functionalized on a 50nm gold nanoparticle (C-AuNP) and a probe DNA functionalized on a 30nm nanoparticle (P-AuNP), creating C-AuNP/t-DNA/P-AuNP conjugates. The C-DNA is complementary to a portion of t-DNA, and PDNA is complementary to the other portion of t-DNA. The parameters that affect the conjugate formation, including hybridization buffer strength, hybridization temperature, and DNA lengths, have been studied to increase the efficiency of the process. It is concluded that the hybridization buffer containing 0.6M NaCl at 50 degrees Celcius consistently formed the nanostructures of CAuNP/t-DNA/P-AuNP conjugates. The hybridization time for each of C-AuNP/t-DNA and P-AuNP/tDNA hybridization was 10 minutes. The optimum nanosatellite formations were obtained when50 nm AuNPs were functionalized with a mixture of 48-base C-DNA and 18-base spacer DNA and30 nm AuNPs were functionalized with a mixture of 46-base P-DNA and 18-base spacer DNA. This sequence-specific controlled formation of nanosatellite structures can be potentially implemented into many fields, such as detection of DNA and RNA of specific pathogens, food safety, and clinical and forensic research.