Strategies in Size-exclusion Chromatography

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Publisher :
ISBN 13 :
Total Pages : 440 pages
Book Rating : 4.3/5 (91 download)

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Book Synopsis Strategies in Size-exclusion Chromatography by : Martin Potschka

Download or read book Strategies in Size-exclusion Chromatography written by Martin Potschka and published by . This book was released on 1996 with total page 440 pages. Available in PDF, EPUB and Kindle. Book excerpt: Examines size exclusion chromatography technology from both the detector-focused and column-focused approaches. Provides fundamental information on the mechanism of size exclusion chromatography Reparations. Addresses special topics in size exclusion chromatography, including characterization of copolymers, inverse size exclusion chromatography, aggregating polymers, polysaccharides, and proteins.

Column Handbook for Size Exclusion Chromatography

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Author :
Publisher : Academic Press
ISBN 13 : 0127655557
Total Pages : 664 pages
Book Rating : 4.1/5 (276 download)

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Book Synopsis Column Handbook for Size Exclusion Chromatography by : Chi-san Wu

Download or read book Column Handbook for Size Exclusion Chromatography written by Chi-san Wu and published by Academic Press. This book was released on 1999 with total page 664 pages. Available in PDF, EPUB and Kindle. Book excerpt: The column is the heart of a chromatographic system. For the first time, complete and authoritative information on columns for size exclusion and gel filtration chromatography is available in a single volume containing contributions from every major manufacturer worldwide and many renowned column users. This comprehensive handbook covers the technology, characterization, application, evaluation, maintenance, and quality control of commercial analytical and preparative columns for SEC, GFC, and two closely related techniques, hydrodynamic chromatography and high osmotic pressure chromatography. Experienced column users discuss various applications in organic and aqueous mobile phases for synthetic and biopolymers, small solutes, biopharmaceutical therapeutics, and high-temperature SEC.

Size Exclusion Chromatography

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Publisher : Springer Science & Business Media
ISBN 13 : 1461578612
Total Pages : 292 pages
Book Rating : 4.4/5 (615 download)

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Book Synopsis Size Exclusion Chromatography by : B. J. Hunt

Download or read book Size Exclusion Chromatography written by B. J. Hunt and published by Springer Science & Business Media. This book was released on 2013-04-17 with total page 292 pages. Available in PDF, EPUB and Kindle. Book excerpt: There is a large and increasing variety of polymers currently in use both for domestic and industrial applications. The properties of polymers are deter mined not only by their chemical type, but also by their molecular mass and molecular mass distributions. However, while the chemical type of polymers can be determined relatively easily, the average molecular masses and molecular mass distributions are more difficult to measure. The molecular mass averages of a polymer are measured by specialized and complex techniques such as light scattering (for weight average) and osmometry (for number average). Thus, complete characterization of the molecular mass distribution of a polymer by such means requires separating the sample into many fractions which can then be examined individually. Since size exclusion chromatography was introduced as a rapid and straightforward technique for the characterization of polymer molecular mass distributions, there have been tremendous increases in development and applications, and it was felt appropriate to bring together into a single volume the information required by scientists from many disciplines who wish to use the technique. This book should be useful to existing users, those who are new to the technique, and those who may be familiar with the basic technique and now wish to extend their capabilities to more complex applications (or to consider the potential of a number of related techniques). The book will also be of general interest to the experienced liquid chromatographer.

Handbook Of Size Exclusion Chromatography And Related Techniques

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Author :
Publisher : CRC Press
ISBN 13 : 0203913329
Total Pages : 720 pages
Book Rating : 4.2/5 (39 download)

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Book Synopsis Handbook Of Size Exclusion Chromatography And Related Techniques by : Chi-San Wu

Download or read book Handbook Of Size Exclusion Chromatography And Related Techniques written by Chi-San Wu and published by CRC Press. This book was released on 2003-11-04 with total page 720 pages. Available in PDF, EPUB and Kindle. Book excerpt: Documenting critical advances in this rapidly evolving field, the Second Edition highlights the need for new applications and technologies that assist in the determination of molecular weight and molecular weight distributions of polymers in an accurate, efficient manner. This volume presents the latest findings from a international team of specialists and continues to inspire and extend practical applications of size exclusion chromatography (SEC). It includes six new chapters covering high-speed size exclusion chromatography, SEC of low molecular weight materials, and the extended family of techniques, from two-dimensional liquid chromatography to high osmotic pressure chromatography.

Size Exclusion Chromatography

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Publisher : Springer Science & Business Media
ISBN 13 : 3662039109
Total Pages : 236 pages
Book Rating : 4.6/5 (62 download)

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Book Synopsis Size Exclusion Chromatography by : Sadao Mori

Download or read book Size Exclusion Chromatography written by Sadao Mori and published by Springer Science & Business Media. This book was released on 2013-03-09 with total page 236 pages. Available in PDF, EPUB and Kindle. Book excerpt: Although size exclusion chromatography (SEC) is perhaps the most popular and widely used technique for determining the molecular weight distribution of polymeric materials, there have been very few texts written on this topic. During the past decade, SEC has experienced a considerable amount of growth in regard to column and detector technology and new applications. With these advances, SEC can now be used for determining absolute molecular weight, polymer chain conformation and size, and branching, as well as polymer solution properties. This book introduces the reader to the fundamentals of SEC with emphasis on practical aspects of the technique, such as column and mobile selection, calibration, new detector capabilities and guidelines for performing SEC on most types of polymers, especially those of industrial importance. This book is intended for either those new to the field of SEC, or for those research workers who require a more comprehensive background.

Biological Small Angle Scattering: Techniques, Strategies and Tips

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Author :
Publisher : Springer
ISBN 13 : 981106038X
Total Pages : 269 pages
Book Rating : 4.8/5 (11 download)

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Book Synopsis Biological Small Angle Scattering: Techniques, Strategies and Tips by : Barnali Chaudhuri

Download or read book Biological Small Angle Scattering: Techniques, Strategies and Tips written by Barnali Chaudhuri and published by Springer. This book was released on 2017-12-07 with total page 269 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book provides a clear, comprehensible and up-to-date description of how Small Angle Scattering (SAS) can help structural biology researchers. SAS is an efficient technique that offers structural information on how biological macromolecules behave in solution. SAS provides distinct and complementary data for integrative structural biology approaches in combination with other widely used probes, such as X-ray crystallography, Nuclear magnetic resonance, Mass spectrometry and Cryo-electron Microscopy. The development of brilliant synchrotron small-angle X-ray scattering (SAXS) beam lines has increased the number of researchers interested in solution scattering. SAS is especially useful for studying conformational changes in proteins, highly flexible proteins, and intrinsically disordered proteins. Small-angle neutron scattering (SANS) with neutron contrast variation is ideally suited for studying multi-component assemblies as well as membrane proteins that are stabilized in surfactant micelles or vesicles. SAS is also used for studying dynamic processes of protein fibrillation in amyloid diseases, and pharmaceutical drug delivery. The combination with size-exclusion chromatography further increases the range of SAS applications. The book is written by leading experts in solution SAS methodologies. The principles and theoretical background of various SAS techniques are included, along with practical aspects that range from sample preparation to data presentation for publication. Topics covered include techniques for improving data quality and analysis, as well as different scientific applications of SAS. With abundant illustrations and practical tips, we hope the clear explanations of the principles and the reviews on the latest progresses will serve as a guide through all aspects of biological solution SAS. The scope of this book is particularly relevant for structural biology researchers who are new to SAS. Advanced users of the technique will find it helpful for exploring the diversity of solution SAS methods and applications. Chapter 3 of this book is available open access under a CC BY 4.0 license at link.springer.com.

Modern Size-Exclusion Liquid Chromatography

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Author :
Publisher : John Wiley & Sons
ISBN 13 : 0470442832
Total Pages : 512 pages
Book Rating : 4.4/5 (74 download)

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Book Synopsis Modern Size-Exclusion Liquid Chromatography by : Andre Striegel

Download or read book Modern Size-Exclusion Liquid Chromatography written by Andre Striegel and published by John Wiley & Sons. This book was released on 2009-07-31 with total page 512 pages. Available in PDF, EPUB and Kindle. Book excerpt: The Second Edition of Modern Size-Exclusion Chromatography offers a complete guide to the theories, methods, and applications of size-exclusion chromatography. It provides an unparalleled, integrated, up-to-date treatment of gel permeation and gel filtration chromatography. With its detailed descriptions of techniques, data handling, compilations of information on columns and column packings, and tables of important solvents and reference materials, the book offers readers everything they need to take full advantage of this popular macromolecular characterization technique. Since publication of the first edition in 1979, there have been many important advances in the field of size-exclusion chromatography. This Second Edition brings the book thoroughly up to date, with expert coverage of: New and emerging industrial and research applications Practical aspects of size-exclusion chromatography (SEC) and multidetector and multidimensional SEC technologies for polymer architecture and copolymer analysis Updated information on the latest equipment and techniques New best practices for the lab SEC in relation to polymer characterization techniques such as GPEC, LCCC, and rheology Throughout the text, detailed examples guide you step by step through all the latest techniques and applications. With its extensive revisions and updates written by leading experts and pioneers in the field, Modern Size-Exclusion Liquid Chromatography remains the definitive resource for the broad range of researchers and scientists who use HPLC and GPC methods.

Aqueous Size-Exclusion Chromatography

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Author :
Publisher : Elsevier
ISBN 13 : 0080858449
Total Pages : 473 pages
Book Rating : 4.0/5 (88 download)

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Book Synopsis Aqueous Size-Exclusion Chromatography by : P.L. Dubin

Download or read book Aqueous Size-Exclusion Chromatography written by P.L. Dubin and published by Elsevier. This book was released on 1988-05-01 with total page 473 pages. Available in PDF, EPUB and Kindle. Book excerpt: The rapid development of new packings for aqueous size-exclusion chromatography has revolutionized this field. High resolution non-adsorptive columns now make possible the efficient separation of proteins and the rapid and precise determination of the molecular weight distribution of synthetic polymers. This technology is also being applied to the separation of small ions, the characterization of associating systems, and the measurement of branching. At the same time, fundamental studies are elucidating the mechanisms of the various chromatographicprocesses.These developments in principles and applications are assembled for the first time in this book. Fundamental issues are dealt with: the roles of pore structure and macromolecular dimensions, hydrophobic and electrostatic effects, and the determination and control of column efficiency. High-performance packings based on derivatized silica are reviewed in detail. Special techniques are thoroughly described, including SEC/LALLS, inverse exclusion chromatography, and frontal zone chromatography. Attention is focussed on special applications of size-exclusion methods, such as the characterization of micelles, separations of inorganic ions, and Hummel-Dreyer and related methods for equilibrium systems. Protein chromatography is dealt with in both dedicated sections and throughout the book as a whole.This is a particularly comprehensive and authoritative work - all the contributions review broad topics of general significance and the authors are of high repute. The material will be of special value for the characterization of synthetic water-soluble polymers, especially polyelectrolytes. Biochemists will find fundamental and practical guidance on protein separations. Researchers confronted with solutes that exhibit complex chromatographic behavior, such as humic acids, aggregating proteins, and micelles should find the contents of this volume illuminating.

Crystallization of Membrane Proteins

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Publisher : CRC Press
ISBN 13 : 1351088173
Total Pages : 343 pages
Book Rating : 4.3/5 (51 download)

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Book Synopsis Crystallization of Membrane Proteins by : Hartmut Michel

Download or read book Crystallization of Membrane Proteins written by Hartmut Michel and published by CRC Press. This book was released on 2018-01-18 with total page 343 pages. Available in PDF, EPUB and Kindle. Book excerpt: The precise knowledge of the structure of biological macromolecules forms the basis of understanding their function and their mechanism of action. It also lays the foundation for rational protein and drug design. The only method to obtain this knowledge is still crystallography. At present, the structures of about 400 proteins are known at or nearly at atomic proteins. However, only two of them are membrane proteins or complexes of the membrane proteins. The reasons for the difference is not the crystals of membrane proteins resists forming special problems when being analysed. The reason is that the membrane proteins resist into forming into well-ordered crystals. The intention of this book is to help to produce well-ordered crystals proteins and to provide guidelines, it is aimed at both biochemists and protein crystallographer‘s.

Size Exclusion Chromatography

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Author :
Publisher : Springer
ISBN 13 : 9781461578628
Total Pages : 286 pages
Book Rating : 4.5/5 (786 download)

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Book Synopsis Size Exclusion Chromatography by : B. J. Hunt

Download or read book Size Exclusion Chromatography written by B. J. Hunt and published by Springer. This book was released on 2014-01-10 with total page 286 pages. Available in PDF, EPUB and Kindle. Book excerpt: There is a large and increasing variety of polymers currently in use both for domestic and industrial applications. The properties of polymers are deter mined not only by their chemical type, but also by their molecular mass and molecular mass distributions. However, while the chemical type of polymers can be determined relatively easily, the average molecular masses and molecular mass distributions are more difficult to measure. The molecular mass averages of a polymer are measured by specialized and complex techniques such as light scattering (for weight average) and osmometry (for number average). Thus, complete characterization of the molecular mass distribution of a polymer by such means requires separating the sample into many fractions which can then be examined individually. Since size exclusion chromatography was introduced as a rapid and straightforward technique for the characterization of polymer molecular mass distributions, there have been tremendous increases in development and applications, and it was felt appropriate to bring together into a single volume the information required by scientists from many disciplines who wish to use the technique. This book should be useful to existing users, those who are new to the technique, and those who may be familiar with the basic technique and now wish to extend their capabilities to more complex applications (or to consider the potential of a number of related techniques). The book will also be of general interest to the experienced liquid chromatographer.

Multiple Detection in Size-exclusion Chromatography

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Publisher :
ISBN 13 :
Total Pages : 370 pages
Book Rating : 4.3/5 (91 download)

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Book Synopsis Multiple Detection in Size-exclusion Chromatography by : André M. Striegel

Download or read book Multiple Detection in Size-exclusion Chromatography written by André M. Striegel and published by . This book was released on 2005 with total page 370 pages. Available in PDF, EPUB and Kindle. Book excerpt: For four decades, size-exclusion chromatography has played a prominent role in characterizing polymers, by determining the polymer's molar mass averages, often not absolutely but relative to some calibration standard. Not satisfied, scientists now want to determine the absolute molar mass averages and distribution and to characterize their long-chain and short-chain branching, tacticity, copolymer and base-pair sequences, and other matters. Oh yes, and they want all this as a continuous function of the molar mass of the analyte. Fortunately, the technique is endowed with a number of detection methods, and the 18 papers here explain the role of those various detection methods and the synergistic effect of combining them in different configurations to obtain desired results. Distributed in the US by Oxford University Press. Annotation : 2004 Book News, Inc., Portland, OR (booknews.com).

Handbook Of Size Exclusion Chromatography And Related Techniques

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Author :
Publisher : CRC Press
ISBN 13 : 9780203913321
Total Pages : 720 pages
Book Rating : 4.9/5 (133 download)

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Book Synopsis Handbook Of Size Exclusion Chromatography And Related Techniques by : Chi-San Wu

Download or read book Handbook Of Size Exclusion Chromatography And Related Techniques written by Chi-San Wu and published by CRC Press. This book was released on 2003-11-04 with total page 720 pages. Available in PDF, EPUB and Kindle. Book excerpt: Documenting critical advances in this rapidly evolving field, the Second Edition highlights the need for new applications and technologies that assist in the determination of molecular weight and molecular weight distributions of polymers in an accurate, efficient manner. This volume presents the latest findings from a international team of specialists and continues to inspire and extend practical applications of size exclusion chromatography (SEC). It includes six new chapters covering high-speed size exclusion chromatography, SEC of low molecular weight materials, and the extended family of techniques, from two-dimensional liquid chromatography to high osmotic pressure chromatography.

Liquid Chromatography

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Publisher : Elsevier Inc. Chapters
ISBN 13 : 0128071362
Total Pages : 53 pages
Book Rating : 4.1/5 (28 download)

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Book Synopsis Liquid Chromatography by : A.M. Striegel

Download or read book Liquid Chromatography written by A.M. Striegel and published by Elsevier Inc. Chapters. This book was released on 2013-01-08 with total page 53 pages. Available in PDF, EPUB and Kindle. Book excerpt: Size-exclusion chromatography (SEC) is used for the characterization and/or separation of macromolecules. Size-based sampling of column pore volume provides information in terms of the molar mass averages and distributions of disperse polymers. The history of the development, the basic theory of column separations, calibration, and detection strategies for obtaining absolute molar mass information are described, as are modern multidetector techniques and the incorporation of SEC into two-dimensional liquid chromatographic arrangements.

Handbook of Size Exclusion Chromatography

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Author :
Publisher : CRC Press
ISBN 13 : 9780824792886
Total Pages : 453 pages
Book Rating : 4.7/5 (928 download)

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Book Synopsis Handbook of Size Exclusion Chromatography by : Chi-San Wu

Download or read book Handbook of Size Exclusion Chromatography written by Chi-San Wu and published by CRC Press. This book was released on 1995-01-06 with total page 453 pages. Available in PDF, EPUB and Kindle. Book excerpt: This work details the practical use of size exclusion chromatography (SEC) in characterizing the molecular weight distribution of important polymeric materials. It addresses problems encountered in the SEC of specific substances, including copolymers, polyamides, polyvinyl alcohol and acetate, lignin derivatives, proteins and starch.

Modern Size-Exclusion Liquid Chromatography

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Author :
Publisher : Wiley
ISBN 13 : 0470442832
Total Pages : 496 pages
Book Rating : 4.4/5 (74 download)

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Book Synopsis Modern Size-Exclusion Liquid Chromatography by : Andre Striegel

Download or read book Modern Size-Exclusion Liquid Chromatography written by Andre Striegel and published by Wiley. This book was released on 2009-07-31 with total page 496 pages. Available in PDF, EPUB and Kindle. Book excerpt: The Second Edition of Modern Size-Exclusion Chromatography offers a complete guide to the theories, methods, and applications of size-exclusion chromatography. It provides an unparalleled, integrated, up-to-date treatment of gel permeation and gel filtration chromatography. With its detailed descriptions of techniques, data handling, compilations of information on columns and column packings, and tables of important solvents and reference materials, the book offers readers everything they need to take full advantage of this popular macromolecular characterization technique. Since publication of the first edition in 1979, there have been many important advances in the field of size-exclusion chromatography. This Second Edition brings the book thoroughly up to date, with expert coverage of: New and emerging industrial and research applications Practical aspects of size-exclusion chromatography (SEC) and multidetector and multidimensional SEC technologies for polymer architecture and copolymer analysis Updated information on the latest equipment and techniques New best practices for the lab SEC in relation to polymer characterization techniques such as GPEC, LCCC, and rheology Throughout the text, detailed examples guide you step by step through all the latest techniques and applications. With its extensive revisions and updates written by leading experts and pioneers in the field, Modern Size-Exclusion Liquid Chromatography remains the definitive resource for the broad range of researchers and scientists who use HPLC and GPC methods.

Optimization in HPLC

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Publisher : John Wiley & Sons
ISBN 13 : 3527347895
Total Pages : 418 pages
Book Rating : 4.5/5 (273 download)

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Book Synopsis Optimization in HPLC by : Stavros Kromidas

Download or read book Optimization in HPLC written by Stavros Kromidas and published by John Wiley & Sons. This book was released on 2021-11-08 with total page 418 pages. Available in PDF, EPUB and Kindle. Book excerpt: Learn to maximize the performance of your HPLC or UHPLC system with this resource from leading experts in the field Optimization in HPLC: Concepts and Strategies delivers tried-and-tested strategies for optimizing the performance of HPLC and UHPLC systems for a wide variety of analytical tasks. The book explains how to optimize the different HPLC operation modes for a range of analyses, including small molecules, chiral substances, and biomolecules. It also shows readers when and how computational tools may be used to optimize performance. The practice-oriented text describes common challenges faced by users and developers of HPLC and UHPLC systems, as well as how those challenges can be overcome. Written for first-time and experienced users of HPLC technology and keeping pace with recent developments in HPLC instrumentation and operation modes, this comprehensive guide leaves few questions unanswered. Readers will also benefit from the inclusion of: A thorough introduction to optimization strategies for different modes and uses of HPLC, including working under regulatory constraints An exploration of computer aided HPLC optimization, including ChromSwordAuto and Fusion QbD A treatment of current challenges for HPLC users in industry as well as large and small analytical service providers Discussions of current challenges for HPLC equipment suppliers Tailor-made for analytical chemists, chromatographers, pharmacologists, toxicologists, and lab technicians, Optimization in HPLC: Concepts and Strategies will also earn a place on the shelves of analytical laboratories in academia and industry who seek a one-stop reference for optimizing the performance of HPLC systems.

Aqueous Size-exclusion Chromatography

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Author :
Publisher :
ISBN 13 : 9780444416162
Total Pages : 453 pages
Book Rating : 4.4/5 (161 download)

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Book Synopsis Aqueous Size-exclusion Chromatography by : Paul Dubin

Download or read book Aqueous Size-exclusion Chromatography written by Paul Dubin and published by . This book was released on 1988 with total page 453 pages. Available in PDF, EPUB and Kindle. Book excerpt: