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Author : Jack Glaser
Publisher : MBF Press
ISBN 13 : 0978647114
Total Pages : 103 pages
Book Rating : 4.9/5 (786 download)
Download or read book Stereology for Biological Research written by Jack Glaser and published by MBF Press. This book was released on 2007 with total page 103 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author : Vyvyan Howard
Publisher : Garland Science
ISBN 13 : 0203006399
Total Pages : 277 pages
Book Rating : 4.2/5 (3 download)
Download or read book Unbiased Stereology written by Vyvyan Howard and published by Garland Science. This book was released on 2004-11-15 with total page 277 pages. Available in PDF, EPUB and Kindle. Book excerpt: Unbiased Stereology, Second Edition is a practical guide to making unbiased 3-D measurements via the microscope. Only those stereological techniques which have been tried and tested by real application are included. Although this technology is essentially mathematical and statistical, the authors do not immerse the reader in complex analysis, but rather provide simple heuristic explanations and references to the original proof, and illustrate the theory by analogies drawn from everyday experience. To give practical experience in application of the techniques, exercises are provided at the end of each chapter, complete with detailed worked answers.
Author : Mark J. West
Publisher :
ISBN 13 : 9781936113606
Total Pages : 0 pages
Book Rating : 4.1/5 (136 download)
Download or read book Basic Stereology for Biologists and Neuroscientists written by Mark J. West and published by . This book was released on 2012 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Stereological techniques allow biologists to create quantitative, three-dimensional descriptions of biological structures from two- dimensional images of tissue viewed under the microscope. For example, they can accurately estimate the size of a particular organelle, the total length of a mass of capillaries, or the number of neurons or synapses in a particular region of the brain. This book provides a practical guide to designing and critically evaluating stereological studies of the nervous system and other tissues. It explains the basic concepts behind design-based stereology and how to get started. Also included are detailed descriptions of how to prepare tissue appropriately, perform pilot studies and decide on the appropriate sampling strategy, and account for phenomena such as tissue shrinkage. Numerous examples of applications of stereological methods that are applicable to studies of the central system and a wide variety of other tissues are explained. The book is therefore essential reading for neurobiologists and cell biologists interested in generating accurate representations of cell and tissue architecture.
Author : John C. Russ
Publisher : Springer Science & Business Media
ISBN 13 : 1489935339
Total Pages : 192 pages
Book Rating : 4.4/5 (899 download)
Download or read book Practical Stereology written by John C. Russ and published by Springer Science & Business Media. This book was released on 2013-11-09 with total page 192 pages. Available in PDF, EPUB and Kindle. Book excerpt: vi on geometric probability is included, students can be expected to create a few simple programs like those shown, but for other geometries. I am indebted to Tom Hare for critical reviews of the material and an endless enthusiasm to debate and derive stereological relationships; to John Matzka at Plenum Press for patiently instructing me in the intricacies of typesetting; to Chris Russ for helping to program many of these measurement techniques; and especially to Helen Adams, both for her patience with my creative fever to write yet another book, and for pointing out that the title, which I had intended to contrast to "theoretical stereology," can also be understood as the antonym of "impractical stereology." John C. Russ Raleigh,NC July, 1986 Chapter 1: Statistics 1 Accuracy and precision 1 The mean and standard deviation 5 Distributions 7 Comparison 13 Correlation 18 Nonlinear fitting 19 Chapter 2: Image Types 23 Planar sections 23 Projected images 25 Finite sections 28 Space-filling structures and dispersed phases 29 Types of images and contrast mechanisms 31 Sampling 32 Chapter 3: Manual Methods 35 Volume fraction 35 Surface density 38 Contiguity 41 Mean intercept length 42 Line density 43 Grain size determination 55 Curvature 48 Reticles to aid counting 49 Magnification and units 51 Chapter4: Size Distributions 53 Intercept length in spheres 53 Nonspherical shapes 57 Corrections for finite section thickness 59 Lamellae 61 Measurement of profile size 62 Nonspherical particles 69 vii Contents viii Chapter 5: Computer Metlwds 73
Author : Peter R. Mouton
Publisher : JHU Press
ISBN 13 : 0801899850
Total Pages : 195 pages
Book Rating : 4.8/5 (18 download)
Download or read book Unbiased Stereology written by Peter R. Mouton and published by JHU Press. This book was released on 2011-07-19 with total page 195 pages. Available in PDF, EPUB and Kindle. Book excerpt: This update to Peter R. Mouton's pioneering work provides bioscientists with the concepts needed in order to apply the principles and practices of unbiased stereology to research involving biological tissues. Mouton starts with a brief explanation of the history and theory of the process before defining the terms, concepts, and tools of unbiased stereological procedures. He compares and contrasts the procedures with less-exacting approaches to quantitative analysis of biological structure using specific examples from biomedical literature. The book incorporates existing best practices with new methodologies, such as the Rare Event Protocol, while simplifying the dense, often difficult literature on the Subject to show the utility and importance of unbiased stereology. This clear, insightful guide goes a step further than other books on this Subject by demonstrating not only how to use unbiased stereology but also how to interpret and present the results. Written by the official U.S. representative to the International Society for Stereology, this is the most complete, up-to-date resource on the science of unbiased stereology. Those new to bioscience research as well as experienced practitioners will find that Mouton's explanations are the perfect companion for stereology courses and workshops.
Author : Peter R. Mouton
Publisher : JHUP
ISBN 13 :
Total Pages : 236 pages
Book Rating : 4.3/5 (91 download)
Download or read book Principles and Practices of Unbiased Stereology written by Peter R. Mouton and published by JHUP. This book was released on 2002-05-16 with total page 236 pages. Available in PDF, EPUB and Kindle. Book excerpt: "An important book for the libraries of professionals in neurobiology, neurology, and neurosurgery." -- Doody's Book Review Service
Author : John C. Russ
Publisher : Springer Science & Business Media
ISBN 13 : 1461512336
Total Pages : 384 pages
Book Rating : 4.4/5 (615 download)
Download or read book Practical Stereology written by John C. Russ and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 384 pages. Available in PDF, EPUB and Kindle. Book excerpt: Stereology is the science that relates three-dimensional structure to the two-dimensional images that can be measured. The most common field of application is in microscopy, both of man-made materials (metals, ceramics, composites, etc.) and of biological tissue samples. This book covers the applications and terminology of both fields. Recent emphasis in stereology is concerned with sampling strategies to avoid bias due to directionality and non-uniformity, and these methods are fully covered. So are the classic techniques that measure size distributions, surface curvature, etc., which are widely useful but not discussed in other recent texts. Finally, most stereology is taught as manual procedures using counting and grids, but this text also covers the use of modern desktop computers for image analysis and processing to obtain and interpret the stereological data.
Author : P. R. Mouton
Publisher : John Wiley & Sons
ISBN 13 : 1118444183
Total Pages : 305 pages
Book Rating : 4.1/5 (184 download)
Download or read book Neurostereology written by P. R. Mouton and published by John Wiley & Sons. This book was released on 2013-11-12 with total page 305 pages. Available in PDF, EPUB and Kindle. Book excerpt: Stereological methods provide researchers with unparalleled quantitative data from tissue samples and allow for well-evidenced research advances in a broad range of scientific fields. Presenting a concise introduction to the methodology and application of stereological research in neuroscience, Neurostereology provides a fuller understanding of the use of these methods in research and a means for replicating successful scientific approaches. Providing sound footing for future research, Neurostereology is a useful tool for basic and clinical researchers and advanced students looking to integrate these methods into their research.
Download or read book The Applications of Stereology and Computing in Earth Sciences Metallography and Biological Research, Abstracts written by and published by . This book was released on 1976 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author : Walter Pfaller
Publisher : Springer Science & Business Media
ISBN 13 : 3642682871
Total Pages : 183 pages
Book Rating : 4.6/5 (426 download)
Download or read book Structure Function Correlation on Rat Kidney written by Walter Pfaller and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 183 pages. Available in PDF, EPUB and Kindle. Book excerpt: Over the past few decades an exceedingly large number of experimental and clinical investigations have been performed in an attempt to analyze the way in which the kidney functions. The basis for all this work was established during the nineteenth and the early twentieth century by morphologists (Bowman 1842; Hyrtl1863, 1872; Heidenhain 1874; Peter 1909; von Mollendorf 1930). All these investigators clearly outlined the extremely heterogeneous assembly of renal tissue and also defined the nephron as the smallest morphological unit. It was further the merit of these anato mists and histologists to preclude quite a number of nephron functions based merely on their careful observations. Contemporary histologists have been able to add little to these observations. Unfortunately with the introduction of physiologic in vivo et situ studies on kidneys the interest in heterogeneity waned. This lack of attention was aggravated by the introduction of the clearance techniques which cannot account for regional differences in the function of the smallest unit, the nephron. That ana tomic heterogeneity has a functional correlate was strongly suggested by Trueta et al. (1947) and vigorously stimulated a number of studies. The development of physiologic microtechniques, like micropuncture and microperfusion of single nephrons, or the perfusion of isolated nephron portions and electrophysiologic studies, enormously expanded our knowledge concerning details regarding nephron and total renal func tion.
Author : Stephen M. Evans
Publisher :
ISBN 13 : 9780198505280
Total Pages : 362 pages
Book Rating : 4.5/5 (52 download)
Download or read book Quantitative Methods in Neuroscience written by Stephen M. Evans and published by . This book was released on 2004 with total page 362 pages. Available in PDF, EPUB and Kindle. Book excerpt: Stereology is a valuable tool for neuroscientists, allowing them to obtain 3-Dimensional information from 2-Dimensional measurements made on appropriately sampled sections (usually obtained from histological sections or MRI/CT/PET scans). This 3-D information is invaluable in correlatingstructural/functional relationships in the pursuit of far greater understanding of the function of the central nervous system. However, in carrying out such measurements, often based on limited data sets, there is a risk of experimenter bias. An important feature of modern design based stereology isto be aware of potential sources of bias and eliminate them during the data collection. With many of the major neuroscience journals now insisting that quantitative data be presented, there is a greater need than ever for neuroscientists to understand the theory and practice behind quantitativemethods, such as those offered by stereology. Quantitative Methods in Neuroscience is a cookbook of stereological methods written especially for neuroscientists. It provides clear and accessible advice about when and when not to use stereology. Throughout the book, the emphasis is on practical guidance, rather than discussions and formulae.Written by leading scientists in the field of stereology, with a Foreword by D.C. Sterio, the book will be a valuable introduction to these methods for neuroscientists, and all those involved in development of new drug programmes.
Author : Adrian Baddeley
Publisher : CRC Press
ISBN 13 : 1135438366
Total Pages : 412 pages
Book Rating : 4.1/5 (354 download)
Download or read book Stereology for Statisticians written by Adrian Baddeley and published by CRC Press. This book was released on 2004-11-29 with total page 412 pages. Available in PDF, EPUB and Kindle. Book excerpt: Setting out the principles of stereology from a statistical viewpoint, this book focuses on both basic theory and practical implications. The authors discuss ways to effectively communicate statistical issues to clients, draw attention to common methodological errors, and provide references to essential literature. The first full text on design-based stereology opens with a review of classical and modern stereology, followed by a treatment of mathematical foundations and then on to core techniques. The final chapters discuss implementing techniques in practical sampling designs, summarize understanding of the variance of stereological estimators, and describe open problems for further research. The book also details isotropic, vertical or local sampling designs for estimating stereological parameters such as volume, surface area, particle number and spatial distribution. This extensive text offers support to statistical consultants using examples, applications and unique Advice to Consultants sections. It contains numerous literature references, bibliographic notes and nearly 150 illustrations.
Author : Hans Elias
Publisher : Springer Science & Business Media
ISBN 13 : 3642882609
Total Pages : 331 pages
Book Rating : 4.6/5 (428 download)
Download or read book Stereology written by Hans Elias and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 331 pages. Available in PDF, EPUB and Kindle. Book excerpt: The Second International Congress for Stereology, again, has brought together scientists from very diverse discipl ines for discussion of problems concerning the recognition of three-dimensional structure, problems which confront those who study materials, rocks, biological systems or heavenly bodies. The program was organized into sessions each dealing with a special type of structural problem regardless of systems in the study of which these problems occur. Since all natural sciences have similar structural questions to investigate, discourses among biologists, metallurgists etc. were intense. Subject areas were not separated during the Congress. No concurrent sessions were held. Each participant had the opportunity to hear every paper. This re sulted in an unusually high attendance. During the last session, after five and a half days of intense work almost half the participants were still present in the lecture hall. Each of us was fascinated with what he was able to learn from fellow stereologists who studied different sectors of nature. Friendshipswere establ ished across oceans and across discipl inary boundaries. Each session was introduced by a key-note lecture didactical, meth odological and theoretical in nature. These key-note lectures can be recognized in this volume by their greater length, 12 pages being allotted to each key-note speaker. Collectively they constitute al most a textbook of stereology. Contributed papers in each problem category deal with appl ications.
Author : P. R. Mouton
Publisher : Wiley-Blackwell
ISBN 13 : 9781118444207
Total Pages : 280 pages
Book Rating : 4.4/5 (442 download)
Download or read book Neurostereology written by P. R. Mouton and published by Wiley-Blackwell. This book was released on 2014-01-17 with total page 280 pages. Available in PDF, EPUB and Kindle. Book excerpt: Stereological methods provide researchers with unparalleled quantitative data from tissue samples and allow for well-evidenced research advances in a broad range of scientific fields. Presenting a concise introduction to the methodology and application of stereological research in neuroscience, Neurostereology provides a fuller understanding of the use of these methods in research and a means for replicating successful scientific approaches. Providing sound footing for future research, Neurostereology is a useful tool for basic and clinical researchers and advanced students looking to integrate these methods into their research.
Author : Anda Cornea
Publisher : Elsevier
ISBN 13 : 0124167136
Total Pages : 261 pages
Book Rating : 4.1/5 (241 download)
Download or read book Fluorescence Microscopy written by Anda Cornea and published by Elsevier. This book was released on 2014-02-24 with total page 261 pages. Available in PDF, EPUB and Kindle. Book excerpt: Fluorescence Microscopy: Super-Resolution and other Novel Techniques delivers a comprehensive review of current advances in fluorescence microscopy methods as applied to biological and biomedical science. With contributions selected for clarity, utility, and reproducibility, the work provides practical tools for investigating these ground-breaking developments. Emphasizing super-resolution techniques, light sheet microscopy, sample preparation, new labels, and analysis techniques, this work keeps pace with the innovative technical advances that are increasingly vital to biological and biomedical researchers. With its extensive graphics, inter-method comparisons, and tricks and approaches not revealed in primary publications, Fluorescence Microscopy encourages readers to both understand these methods, and to adapt them to other systems. It also offers instruction on the best visualization to derive quantitative information about cell biological structure and function, delivering crucial guidance on best practices in related laboratory research. Presents a timely and comprehensive review of novel techniques in fluorescence imaging as applied to biological and biomedical research Offers insight into common challenges in implementing techniques, as well as effective solutions
Author : Douglas J. Taatjes
Publisher : Springer Science & Business Media
ISBN 13 : 1592599931
Total Pages : 505 pages
Book Rating : 4.5/5 (925 download)
Download or read book Cell Imaging Techniques written by Douglas J. Taatjes and published by Springer Science & Business Media. This book was released on 2008-02-04 with total page 505 pages. Available in PDF, EPUB and Kindle. Book excerpt: A diverse collection of state-of-the-art methods for the microscopic imaging of cells and molecules. The authors cover a wide spectrum of complimentary techniques, including such methods as fluorescence microscopy, electron microscopy, atomic force microscopy, and laser scanning cytometry. Additional readily reproducible protocols on confocal scanning laser microscopy, quantitative computer-assisted image analysis, laser-capture microdissection, microarray image scanning, near-field scanning optical microscopy, and reflection contrast microscopy round out this eclectic collection of cutting-edge imaging techniques now available. The authors also discuss preparative methods for particles and cells by transmission electron microscopy.
Author : V. HOWARD
Publisher : BIOS Scientific Publishers
ISBN 13 : 9780387915166
Total Pages : 0 pages
Book Rating : 4.9/5 (151 download)
Download or read book UNBIASED STEREOLOGY written by V. HOWARD and published by BIOS Scientific Publishers. This book was released on 1998-04-03 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: In Unbiased Stereology, the authors present a practical guide to making unbiased 3D measurements using microscopy. Stereology is the statistical inference of geometric parameters from spatially sampled information; it is considered unbiased when the test probes are positioned in an appropriately random manner. Now that unbiased stereology is required for nerve-cell counts in neurotoxicology by the FDA and by many journals before the acceptance of an article or study, this book is essential reading. It includes only those stereological techniques that have been tried and tested by actual application. Specific topics covered include: random sampling and random geometry; the estimation of feature volume, surface, length, number and mean particle size; as well as statistics for stereologists.
