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State Of Art Separation Mass Spectrometry In Protein Characterization
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Book Synopsis Chromatography by : Leonardo Calderon
Download or read book Chromatography written by Leonardo Calderon and published by BoD – Books on Demand. This book was released on 2012-10-24 with total page 442 pages. Available in PDF, EPUB and Kindle. Book excerpt: Nowadays, Chromatography is the most versatile and widespread technique employed in modern chemical analysis and plays a vital role in the advancement of chemistry, biology, medicine and related fields of research. Because of the inherent simplicity and ease of operation, it can be used together with a wide range of detection systems, including electrochemical, photometric and mass spectrometry, being an invaluable laboratory tool for the separation and identification of compounds. The purpose of this book is not only to present the latest state and development tendencies of chromatography, but to bring the reader useful information on separation sciences to enable him to use chromatography on his research field. Taking into account the large amount of knowledge about chromatography theory and practice presented in the book, it has three major parts: applications, theory and sample preparation. The book is also intended for both graduate and postgraduate students in fields such as chemistry, biology, biotechnology, forensic, medicine, pharmacology and engineering, and as a reference for professionals and practitioners.
Book Synopsis Approaches to the Purification, Analysis and Characterization of Antibody-Based Therapeutics by : Allan Matte
Download or read book Approaches to the Purification, Analysis and Characterization of Antibody-Based Therapeutics written by Allan Matte and published by Elsevier. This book was released on 2020-09-07 with total page 222 pages. Available in PDF, EPUB and Kindle. Book excerpt: Approaches to the Purification, Analysis and Characterization of Antibody-Based Therapeutics provides the interested and informed reader with an overview of current approaches, strategies and considerations relating to the purification, analytics and characterization of therapeutic antibodies and related molecules. While there are obviously other books published in and around this subject area, they seem to be either older (c.a. year 2000 publication date) or are more limited in scope. The book will include an extensive bibliography of the published literature in the respective areas covered. It is not, however, intended to be a how-to methods book. Covers the vital new area of R&D on therapeutic antibodies Written by leading scientists and researchers Up-to-date coverage and includes a detailed bibliography
Book Synopsis Characterization of Impurities and Degradants Using Mass Spectrometry by : Guodong Chen
Download or read book Characterization of Impurities and Degradants Using Mass Spectrometry written by Guodong Chen and published by John Wiley & Sons. This book was released on 2011-04-27 with total page 402 pages. Available in PDF, EPUB and Kindle. Book excerpt: The book highlights the current practices and future trends in structural characterization of impurities and degradants. It begins with an overview of mass spectrometry techniques as related to the analysis of impurities and degradants, followed by studies involving characterization of process related impurities (including potential genotoxic impurities), and excipient related impurities in formulated products. Both general practitioners in pharmaceutical research and specialists in analytical chemistry field will benefit from this book that will detail step-by-step approaches and new strategies to solve challenging problems related to pharmaceutical research.
Book Synopsis Proteomics Technologies and Applications by : Ibrokhim Y. Abdurakhmonov
Download or read book Proteomics Technologies and Applications written by Ibrokhim Y. Abdurakhmonov and published by BoD – Books on Demand. This book was released on 2019-09-25 with total page 134 pages. Available in PDF, EPUB and Kindle. Book excerpt: Proteomics Technologies and Applications reviews and describes the nature and application of molecules with proteins or peptides, and elucidates and predicts the possible molecular and physiological causes related to changing proteomic profiles. Chapters target various methods and tools available for analysis, detection, separation, quantification, and localization of cell proteomes of a biological system, which are helpful as biomarkers for various disease prognoses and diagnoses.
Book Synopsis LC-MS/MS in Proteomics by : Pedro R. Cutillas
Download or read book LC-MS/MS in Proteomics written by Pedro R. Cutillas and published by Humana Press. This book was released on 2011-04-06 with total page 330 pages. Available in PDF, EPUB and Kindle. Book excerpt: With the development of new quantitative strategies and powerful bioinformatics tools to cope with the analysis of the large amounts of data generated in proteomics experiments, liquid chromatography with tandem mass spectrometry (LC-MS/MS) is making possible the analysis of proteins on a global scale, meaning that proteomics can now start competing with cDNA microarrays for the analysis of whole genomes. In LC-MS/MS in Proteomics: Methods and Applications, experts in the field provide protocols and up-to-date reviews of the applications of LC-MS/MS, with a particular focus on MS-based methods of protein and peptide quantification and the analysis of post-translational modifications. Beginning with overviews of the use of LC-M/MS in protein analysis, the book continues with topics such as protocols for the analysis of post-translational modifications, with particular focus on phosphorylation and glycosylation, popular techniques for quantitative proteomics, such as multiple reaction monitoring, metabolic labelling, and chemical tagging, biomarker discovery in biological fluids, as well as novel applications of LC-MS/MS. Written in the highly successful Methods in Molecular BiologyTM series format, chapters include introductions to their respective subjects, lists of necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, LC-MS/MS in Proteomics: Methods and Applications presents the techniques and concepts necessary in order to aid proteomic practitioners in the application of LC-MS/MS to essentially any biological problem.
Book Synopsis Proteome and Protein Analysis by : R.M. Kamp
Download or read book Proteome and Protein Analysis written by R.M. Kamp and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 371 pages. Available in PDF, EPUB and Kindle. Book excerpt: The papers selected here cover new, sensitive and rapid methods for the analysis of proteins, with special emphasis on the proteome. In addition to the experimental details, the advantages and limitations of the methodological approaches are discussed, and topics include sequencing analysis, sample preparation, mass spectrometry, NMR, analysis of post-translational modifications, purification of recombinant proteins, protein-protein and protein-DNA interactions, structure prediction, modeling and folding, functional implications of domains and newly emerging investigative methods, allowing analysis of the expression of genes.
Book Synopsis Sector Field Mass Spectrometry for Elemental and Isotopic Analysis by : Thomas Prohaska
Download or read book Sector Field Mass Spectrometry for Elemental and Isotopic Analysis written by Thomas Prohaska and published by Royal Society of Chemistry. This book was released on 2014-11-21 with total page 667 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book was triggered by the success story of sector field mass spectrometry in elemental and isotopic analysis from the early days when the first mass spectrum of Ne was presented a hundred years ago. The outstanding and unique features of sector field mass spectrometry - high sensitivity, high mass resolution and simultaneous multiple ion detection - paved the way for its successful and increasing application in different fields of science. Written, compiled and edited by worldwide renowned experts with profound expertise in sector field mass spectrometry related to elemental and isotopic analysis, this book is intended to provide deep insight into the topic along with fundamental knowledge about elemental and isotopic analysis. Aimed at scientists in the field of natural and life sciences, instrument manufacturers, practitioners and graduate students, this book provides solid information about the methodological background and analytical capabilities of sector field mass spectrometry. A detailed description of peculiarities and an overview of the most relevant applications making use of specific techniques using sector field mass analysers (ICP-MS, GDMS, TIMS, SIMS and IRMS) are given, including a presentation of the currently available commercial instruments. This approach guarantees that readers are thoroughly introduced to and familiarized with the fascinating inter- and transdisciplinary field of sector field mass spectrometry.
Book Synopsis Proteome Analysis by : David W Speicher
Download or read book Proteome Analysis written by David W Speicher and published by Elsevier. This book was released on 2004-05-18 with total page 394 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book explores the current status of proteomics, an exciting new discipline, which is less than 10 years old. This new field has rapidly grown into a major commercial and research enterprise with great prospects for dramatically advancing our knowledge of basic biological and disease processes. The contributors to this book are an international panel of proteomics experts, who review and discuss the current status of specific technologies and approaches. Proteomics represents an exciting new way to pursue biological and biomedical science at an unprecedented pace. Proteomics takes a broad, comprehensive, systematic approach to understanding biology that is generally unbiased and not dependent upon existing knowledge. The major components of proteomics from basic discovery using a range of alternative analytical methods to discovery validation and use for clinical applications are discussed. State-of-the-art protein profiling methods include high resolution two-dimensional gels, two-dimensional differential in-gel electrophoresis, LC-MS and LC-MS/MS using accurate mass tags, and protein identifications of proteins from gels using mass spectrometry methods are discussed in depth. Other chapters describe comprehensive characterization of proteomes using electrophoretic prefractionation and analyses of sub-proteomes based on specific posttranslational modifications including the phospho-proteome, the glyco-proteome, and nitrated proteins. These conventional proteome analysis chapters are complemented by discussion of emerging technologies and approaches such as affinity based biosensor proteomics as well as the use of protein microarrays, microfluidics and nanotechnology. Strategies for improving throughput by automation are also discussed. Additional chapters address the application of current proteome techniques to clinical problems and the availability of protein expression library resources for proteome studies.· Authored by international experts in the field · Covers a wide range of topics including 2-D gels, global proteomics using accurate mass tags, global proteomics using electrophoretic prefractionation, microfluidics, and nanotechnology· Includes state-of-the-art protein profiling methods, and emerging technologies
Book Synopsis Mass Spectrometry for Microbial Proteomics by : Haroun N. Shah
Download or read book Mass Spectrometry for Microbial Proteomics written by Haroun N. Shah and published by John Wiley & Sons. This book was released on 2010-10-28 with total page 545 pages. Available in PDF, EPUB and Kindle. Book excerpt: New advances in proteomics, driven largely by developments in mass spectrometry, continue to reveal the complexity and diversity of pathogenic mechanisms among microbes that underpin infectious diseases. Therefore a new era in medical microbiology is demanding a rapid transition from current procedures to high throughput analytical systems for the diagnosis of microbial pathogens. This book covers the broad microbiological applications of proteomics and mass spectrometry. It is divided into six sections that follow the general progression in which most microbiology laboratories are approaching the subject –Transition, Tools, Preparation, Profiling by Patterns, Target Proteins, and Data Analysis.
Book Synopsis Separation Techniques Applied to Omics Sciences by : Ana Valéria Colnaghi Simionato
Download or read book Separation Techniques Applied to Omics Sciences written by Ana Valéria Colnaghi Simionato and published by Springer Nature. This book was released on 2021-10-09 with total page 277 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book covers liquid chromatography, gas chromatography and capillary electrophoresis, the three main separation techniques lately available, applied to key omic sciences, such as genomics, proteomics, metabolomics and foodomics. The fundamentals of each technique are not covered herein. Instead, the recent advances in such techniques are presented focusing on the application to omics analyses and unique aspects in each case. This volume intends to offer wide ranging options available to researchers on omics sciences, and how to integrate them in order to achieve the comprehension of a biological system as a whole. Omic sciences have been of ultimate importance to comprehend the complex biochemical reactions and related events that occurs upon a biological system. The classical central dogma of molecular biology, which states that genetic information flows unidirectionally from DNA to RNA and then to proteins, has been gradually replaced by the systems biology approach. This book presents a multidisciplinary approach that explains the biological system as a whole, where the entire organism is influenced by a variety of internal events as well as by the environment, showing that each level of the biological information flux may influence the previous or the subsequent one.
Book Synopsis Mass Spectrometry in the Biological Sciences by : A. L. Burlingame
Download or read book Mass Spectrometry in the Biological Sciences written by A. L. Burlingame and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 569 pages. Available in PDF, EPUB and Kindle. Book excerpt: Leading practitioners describe in detail advanced methods of mass spectrometry used in structural characterization of biomacromolecules of both natural and recombinant origin. They demonstrate by example how these methodologies can solve a wide array of real-world problems in protein biochemistry, immunology, and glycobiology, as well as for human bacterial pathogens, lipids, and nucleic acids. The book offers a unique opportunity to learn these techniques that are revolutionizing the field. Its authoritative assessment in the context of how to solve important and challenging problems in bioscience and medicine ensures a competitive advantage for today's researchers.
Book Synopsis Protein Structure Analysis by : Roza Maria Kamp
Download or read book Protein Structure Analysis written by Roza Maria Kamp and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 311 pages. Available in PDF, EPUB and Kindle. Book excerpt: "Protein Structure Analysis - Preparation and Characterization" is a compilation of practical approaches to the structural analysis of proteins and peptides. Here, about 20 authors describe and comment on techniques for sensitive protein purification and analysis. These methods are used worldwide in biochemical and biotechnical research currently being carried out in pharmaceu tical and biomedical laboratories or protein sequencing facilities. The chapters have been written by scientists with extensive ex perience in these fields, and the practical parts are well documen ted so that the reader should be able to easily reproduce the described techniques. The methods compiled in this book were demonstrated in student courses and in the EMBO Practical Course on "Microsequence Analysis of Proteins" held in Berlin September 10-15, 1995. The topics also derived from a FEBS Workshop, held in Halkidiki, Thessaloniki, Greece, in April, 1995. Most of the authors participated in these courses as lecturers and tutors and made these courses extremely lively and successful. Since polypeptides greatly vary depending on their specific structure and function, strategies for their structural analysis must for the most part be adapted to each individual protein. Therefore, advantages and limitations of the experimen tal approaches are discussed here critically, so that the reader becomes familiar with problems that might be encountered.
Book Synopsis Protein Mass Spectrometry by : Julian Whitelegge
Download or read book Protein Mass Spectrometry written by Julian Whitelegge and published by Elsevier. This book was released on 2008-10-09 with total page 563 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book is designed to be a central text for young graduate students interested in mass spectrometry as it relates to the study of protein structure and function as well as proteomics. It is a definite must-have work for:- libraries at academic institutions with Master and Graduate programs in biochemistry, molecular biology, structural biology and proteomics- individual laboratories with interests covering these areas - libraries and individual laboratories in the pharmaceutical and biotechnology industries.*Serves as an essential reference to those working in the field*Incorporates the contributions of prominent experts *Features comprehensive coverage and a logical structure
Book Synopsis Single Cell Analysis by : Dario Anselmetti
Download or read book Single Cell Analysis written by Dario Anselmetti and published by John Wiley & Sons. This book was released on 2009-03-09 with total page 284 pages. Available in PDF, EPUB and Kindle. Book excerpt: The first-ever comprehensive overview of the methods used in this key technology in modern biology provides the latest working knowledge needed by every scientist entering this growing field. It covers all the current technology and application areas, from microscopy and spectroscopy to proteomics and microfluidics.
Download or read book Cumulated Index Medicus written by and published by . This book was released on 1987 with total page 1216 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Molecular Nuclear Medicine by : L.E. Feinendegen
Download or read book Molecular Nuclear Medicine written by L.E. Feinendegen and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 795 pages. Available in PDF, EPUB and Kindle. Book excerpt: Biochemical transparency of the human body is at the doorstep of advanced technology. Toward this goal the book describes relevant isotopic tracer techniques of nuclear medicine. It deals with quantitatively measuring in vivo biochemical reactions as they occur within homeostatic circuits under control by genes and protein interactions. The text indicates how nuclear medicine can aid clinical researchers and practitioners, human geneticists and pharmacologists in understanding (and affecting) gene-phenotype relationships. Experts give background, techniques and examples in an interdisciplinary approach to regional imaging and in vitro analyses of biochemical reactions.
Book Synopsis Multidimensional Liquid Chromatography by : Steven A. Cohen
Download or read book Multidimensional Liquid Chromatography written by Steven A. Cohen and published by John Wiley & Sons. This book was released on 2008-05-09 with total page 490 pages. Available in PDF, EPUB and Kindle. Book excerpt: Multidimensional Liquid Chromatography (MDLC) is a very powerful separation technique for analyzing exceptionally complex samples in one step. This authoritative reference presents a number of recent contributions that help define the current art and science of MDLC. Topics covered include instrumentation, theory, methods development, and applications of MDLC in the life sciences and in industrial chemistry. With the information to help you perform very difficult separations of complex samples, this reference includes chapters contributed by leading experts or teams of experts.
