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Spectral And Imaging Cytometry
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Book Synopsis Spectral and Imaging Cytometry by : Natasha S. Barteneva
Download or read book Spectral and Imaging Cytometry written by Natasha S. Barteneva and published by Springer Nature. This book was released on 2023-04-19 with total page 265 pages. Available in PDF, EPUB and Kindle. Book excerpt: This second edition volume expands on the previous edition with discussions on the latest approaches used in the spectral cytometry field. Beginning with a brief history of spectral cytometry development, this book continues with a section addressing new protocols in different areas of imaging cytometry based on the unique technology of Imagestream and also introduces FlowCam – cytometer with capabilities of analyzing phytoplankton and zooplankton. Written for the highly successful Methods in Molecular Biology series, chapters include brief introductions to their respective topics, complete lists of the materials and reagents necessary, and reproducible laboratory protocols with tips on troubleshooting and recommendations. Cutting-edge and thorough, Spectral and Imaging Flow Cytometry: Methods and Protocols, Second Edition is a valuable resources for beginners and professionals interested in learning more about this developing field. Chapters 1, 2, and 5 are available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.
Book Synopsis Imaging Flow Cytometry by : Natasha S. Barteneva
Download or read book Imaging Flow Cytometry written by Natasha S. Barteneva and published by Humana. This book was released on 2015-11-23 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: This detailed volume for the first time explores techniques and protocols involving quantitative imaging flow cytometry (IFC), which has revolutionized our ability to analyze cells, cellular clusters, and populations in a remarkable fashion. Beginning with an introduction to technology, the book continues with sections addressing protocols for studies on the cell nucleus, nucleic acids, and FISH techniques using an IFC instrument, immune response analysis and drug screening, IFC protocols for apoptosis and cell death analysis, as well as morphological analysis and the identification of rare cells. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Imaging Flow Cytometry: Methods and Protocols will be a critical source for all laboratories seeking to implement IFC in their research studies.
Book Synopsis Multiparameter Flow Cytometry in the Diagnosis of Hematologic Malignancies by : Anna Porwit
Download or read book Multiparameter Flow Cytometry in the Diagnosis of Hematologic Malignancies written by Anna Porwit and published by Cambridge University Press. This book was released on 2018-01-25 with total page 248 pages. Available in PDF, EPUB and Kindle. Book excerpt: Master implementation of the techniques of flow cytometry in diagnosing complex haematological diseases and malignancies in patients, worldwide. Featuring World Health Organization recommendations on pre-analytical steps, instrument settings and panel construction, this invaluable manual offers invaluable support for those researching, practising and analyzing the cause of hematological malignancies. Authored by leading experts, this book puts flow-cytometry into everyday context. With a focus on multicolour panels, the manual provides readers an experienced understanding of effective, implementation techniques. Practitioners of all levels are offered a background in a variety of diseases presented alongside the most current methodology. Wide-ranging and comprehensive; detailed images of healthy blood, bone marrow and lymph-nodes are illustrated throughout, allowing for effective diagnosis. Through engaging with differential diagnoses, the manual offers an understanding of similar symptoms and mimicking malignancies, avoiding inaccurate results. Featuring in-depth descriptions of chronic diseases; users can reach accurate diagnosis, first time.
Book Synopsis Recent Advances in Cytometry, Part A by :
Download or read book Recent Advances in Cytometry, Part A written by and published by Academic Press. This book was released on 2011-06-13 with total page 600 pages. Available in PDF, EPUB and Kindle. Book excerpt: Cytometry is one of the most rapidly growing methodologies available for basic cell and molecular biology, cytogenetics, immunology, oncology, environmental sciences and also various fields of clinical medicine. This new edition, split into 2 Parts, is an almost completely new book, with nearly all of the chapters devoted to new topics. Like the previous volumes on cytometry published as part of the Methods in Cell Biology series, it provides a comprehensive description of particular cytometric methods and reviews their applications. Chapters present the theoretical foundations of the described methods, their applicability in experimental laboratory and clinical settings, and describes common traps and pitfalls such as problems with data interpretation, comparison with alternative assays, and choosing the optimal assay. Comprehensive presentation of cytometric methods covering theoretical applications, applicability, potential pitfalls, and comparisions to alternative assays Discusses many new assays developed since the previous edition Presents recent developments in cytometric intrumentation/technology
Book Synopsis Flow Cytometry Protocols by : Teresa S. Hawley
Download or read book Flow Cytometry Protocols written by Teresa S. Hawley and published by Springer Nature. This book was released on with total page 460 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Fluorescence Lifetime Spectroscopy and Imaging by : Laura Marcu
Download or read book Fluorescence Lifetime Spectroscopy and Imaging written by Laura Marcu and published by CRC Press. This book was released on 2014-07-17 with total page 554 pages. Available in PDF, EPUB and Kindle. Book excerpt: During the past two decades, there has been an increasing appreciation of the significant value that lifetime-based techniques can add to biomedical studies and applications of fluorescence. Bringing together perspectives of different research communities, Fluorescence Lifetime Spectroscopy and Imaging: Principles and Applications in Biomedical Dia
Book Synopsis Advances in Cytometry: Applications by :
Download or read book Advances in Cytometry: Applications written by and published by Elsevier. This book was released on 2024-05-03 with total page 356 pages. Available in PDF, EPUB and Kindle. Book excerpt: Advances in Cytometry: Applications, Volume 176 in the Methods in Cell Biology series, highlights advances in the field, with this new volume presenting interesting chapters on timely topics, including Orthotopic brain tumor models derived from glioblastoma stem-like cells, RNA sequencing in hematopoietic stem cells, Generation of inducible pluripotent stem cells from human dermal fibroblasts, In vitro preparation of dental pulp stem cell grafts combined with biocompatible scaffolds for tissue engineering, Gene expression knockdown in chronic myeloid leukemia stem cells, Identification and isolation of slow-cycling GSCs, Assessment of CD133, EpCAM, and much more. Provides the authority and expertise of leading contributors from an international board of authors Presents the latest release in the Methods in Cell Biology series Includes the latest information on the topic of development, characterization and applications in CAR T Cells
Download or read book Current Protocols on CD-ROM. written by and published by . This book was released on 1997 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Advanced Optical Flow Cytometry by : Valery V. Tuchin
Download or read book Advanced Optical Flow Cytometry written by Valery V. Tuchin and published by John Wiley & Sons. This book was released on 2011-03-31 with total page 698 pages. Available in PDF, EPUB and Kindle. Book excerpt: A detailed look at the latest research in non-invasive in vivo cytometry and its applications, with particular emphasis on novel biophotonic methods, disease diagnosis, and monitoring of disease treatment at single cell level in stationary and flow conditions. This book thus covers the spectrum ranging from fundamental interactions between light, cells, vascular tissue, and cell labeling particles, to strategies and opportunities for preclinical and clinical research. General topics include light scattering by cells, fast video microscopy, polarization, laser-scanning, fluorescence, Raman, multi-photon, photothermal, and photoacoustic methods for cellular diagnostics and monitoring of disease treatment in living organisms. Also presented are discussions of advanced methods and techniques of classical flow cytometry.
Book Synopsis Recent Advances in Cytometry by : Zbigniew Darzynkiewicz
Download or read book Recent Advances in Cytometry written by Zbigniew Darzynkiewicz and published by Academic Press. This book was released on 2011 with total page 617 pages. Available in PDF, EPUB and Kindle. Book excerpt: Cytometry is one of the most rapidly growing methodologies available for basic cell and molecular biology, cytogenetics, immunology, oncology, environmental sciences and also various fields of clinical medicine. This new edition, split into 2 Parts, is an almost completely new book, with nearly all of the chapters devoted to new topics. Like the previous volumes on cytometry published as part of the Methods in Cell Biology series, it provides a comprehensive description of particular cytometric methods and reviews their applications. Chapters present the theoretical foundations of the described methods, their applicability in experimental laboratory and clinical settings, and describes common traps and pitfalls such as problems with data interpretation, comparison with alternative assays, and choosing the optimal assay. Comprehensive presentation of cytometric methods covering theoretical applications, applicability, potential pitfalls, and comparisions to alternative assays * Discusses many new assays developed since the previous edition. Presents recent developments in cytometric intrumentation/technology.
Book Synopsis Biomedical Optical Imaging by : James G. Fujimoto
Download or read book Biomedical Optical Imaging written by James G. Fujimoto and published by Oxford University Press. This book was released on 2009-04-22 with total page 440 pages. Available in PDF, EPUB and Kindle. Book excerpt: Biomedical optical imaging is a rapidly emerging research area with widespread fundamental research and clinical applications. This book gives an overview of biomedical optical imaging with contributions from leading international research groups who have pioneered many of these techniques and applications. A unique research field spanning the microscopic to the macroscopic, biomedical optical imaging allows both structural and functional imaging. Techniques such as confocal and multiphoton microscopy provide cellular level resolution imaging in biological systems. The integration of this technology with exogenous chromophores can selectively enhance contrast for molecular targets as well as supply functional information on processes such as nerve transduction. Novel techniques integrate microscopy with state-of-the-art optics technology, and these include spectral imaging, two photon fluorescence correlation, nonlinear nanoscopy; optical coherence tomography techniques allow functional, dynamic, nanoscale, and cross-sectional visualization. Moving to the macroscopic scale, spectroscopic assessment and imaging methods such as fluorescence and light scattering can provide diagnostics of tissue pathology including neoplastic changes. Techniques using light diffusion and photon migration are a means to explore processes which occur deep inside biological tissues and organs. The integration of these techniques with exogenous probes enables molecular specific sensitivity.
Book Synopsis Handbook of Biological Confocal Microscopy by : James Pawley
Download or read book Handbook of Biological Confocal Microscopy written by James Pawley and published by Springer Science & Business Media. This book was released on 2006-06-02 with total page 1018 pages. Available in PDF, EPUB and Kindle. Book excerpt: Once the second edition was safely off to the printer, the 110 larger world of micro-CT and micro-MRI and the smaller world authors breathed a sigh of relief and relaxed, secure in the belief revealed by the scanning and transmission electron microscopes. that they would “never have to do that again. ” That lasted for 10 To round out the story we even have a chapter on what PowerPoint years. When we ?nally awoke, it seemed that a lot had happened. does to the results, and the annotated bibliography has been In particular, people were trying to use the Handbook as a text- updated and extended. book even though it lacked the practical chapters needed. There As with the previous editions, the editor enjoyed a tremendous had been tremendous progress in lasers and ?ber-optics and in our amount of good will and cooperation from the 124 authors understanding of the mechanisms underlying photobleaching and involved. Both I, and the light microscopy community in general, phototoxicity. It was time for a new book. I contacted “the usual owe them all a great debt of gratitude. On a more personal note, I suspects” and almost all agreed as long as the deadline was still a would like to thank Kathy Lyons and her associates at Springer for year away.
Book Synopsis Fluorescence Applications in Biotechnology and Life Sciences by : Ewa M. Goldys
Download or read book Fluorescence Applications in Biotechnology and Life Sciences written by Ewa M. Goldys and published by John Wiley & Sons. This book was released on 2009-08-24 with total page 398 pages. Available in PDF, EPUB and Kindle. Book excerpt: A self-contained treatment of the latest fluorescence applications in biotechnology and the life sciences This book focuses specifically on the present applications of fluorescence in molecular and cellular dynamics, biological/medical imaging, proteomics, genomics, and flow cytometry. It raises awareness of the latest scientific approaches and technologies that may help resolve problems relevant for the industry and the community in areas such as public health, food safety, and environmental monitoring. Following an introductory chapter on the basics of fluorescence, the book covers: labeling of cells with fluorescent dyes; genetically encoded fluorescent proteins; nanoparticle fluorescence probes; quantitative analysis of fluorescent images; spectral imaging and unmixing; correlation of light with electron microscopy; fluorescence resonance energy transfer and applications; monitoring molecular dynamics in live cells using fluorescence photo-bleaching; time-resolved fluorescence in microscopy; fluorescence correlation spectroscopy; flow cytometry; fluorescence in diagnostic imaging; fluorescence in clinical diagnoses; immunochemical detection of analytes by using fluorescence; membrane organization; and probing the kinetics of ion pumps via voltage-sensitive fluorescent dyes. With its multidisciplinary approach and excellent balance of research and diagnostic topics, this book is an essential resource for postgraduate students and a broad range of scientists and researchers in biology, physics, chemistry, biotechnology, bioengineering, and medicine.
Book Synopsis Flow Cytometry and Cell Sorting by : Andreas Radbruch
Download or read book Flow Cytometry and Cell Sorting written by Andreas Radbruch and published by Springer Science & Business Media. This book was released on 2013-03-14 with total page 365 pages. Available in PDF, EPUB and Kindle. Book excerpt: The analysis and sorting of large numbers of cells with a fluorescence-activated cell sorter (FACS) was first achieved some 30 years ago. Since then, this technology has been rapidly developed and is used today in many laboratories. A Springer Lab Manual Review of the First Edition: "This is a most useful volume which will be a welcome addition for personal use and also for laboratories in a wide range of disciplines. Highly recommended." CYTOBIOS
Book Synopsis Flow Cytometry in Microbiology by : David Lloyd
Download or read book Flow Cytometry in Microbiology written by David Lloyd and published by Springer. This book was released on 2013-11-10 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: As yet, flow cytometry is not used so widely in microbiology as in some other disciplines. This volume presents contributions flow cytometry to study a from research microbiologists who use diverse set of problems. It illustrates the power of the technique, and may persuade others of its usefulness. Most of the con tributors gathered in Cardiff on 23 October 1991, at a meeting organized for the Royal Microscopical Society by Dr. Richard Allman, but the content of their chapters is not limited by the discourse of that meeting, and for balance other experts were invited to write for this book. Flow Cytometry in Microbiology thus represents the first collection of articles specifically devoted to the applications of a technique which promises so much to those investigating the microbial world. Cardiff, 1992 David Lloyd Contents List of Contributors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ix 1 Flow Cytometry: A Technique Waiting for Microbiologists David Lloyd . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1 2 The Physical and Biological Basis for Flow Cytometry of Escherichia coli Erik Boye and Harald B. Steen . . . . . . . . . . . . . . . . . . . . . . 11 3 Flow Cytometric Analysis of Heterogeneous Bacterial Populations Richard Allman, Richard Manchee and David Lloyd. . . . 27 4 On the Determination of the Size of Microbial Cells Using Flow Cytometry Hazel M. Davey, Chris L. Davey and Douglas B. Kell . . 49 5 Uses of Membrane Potential Sensitive Dyes with Bacteria David Mason, Richard Allman and David Lloyd . . . . . . .
Book Synopsis Practical Flow Cytometry by : Howard M. Shapiro
Download or read book Practical Flow Cytometry written by Howard M. Shapiro and published by John Wiley & Sons. This book was released on 2005-02-25 with total page 736 pages. Available in PDF, EPUB and Kindle. Book excerpt: From the reviews of the 3rd Edition... "The standard reference for anyone interested in understandingflow cytometry technology." American Journal of Clinical Oncology "...one of the most valuable of its genre and...addressed to awide audience?written in such an attractive way, being bothinformative and stimulating." Trends in Cell Biology This reference explains the science and discusses the vastbiomedical applications of quantitative analytical cytology usinglaser-activated detection and cell sorting. Now in its fourthedition, this text has been expanded to provide full coverage ofthe broad spectrum of applications in molecular biology andbiotechnology today. New to this edition are chapters on automatedanalysis of array technologies, compensation, high-speed sorting,reporter molecules, and multiplex and apoptosis assays, along withfully updated and revised references and a list of suppliers.
Book Synopsis Coherent Raman Scattering Microscopy by : Ji-Xin Cheng
Download or read book Coherent Raman Scattering Microscopy written by Ji-Xin Cheng and published by CRC Press. This book was released on 2016-04-19 with total page 603 pages. Available in PDF, EPUB and Kindle. Book excerpt: The First Book on CRS MicroscopyCompared to conventional Raman microscopy, coherent Raman scattering (CRS) allows label-free imaging of living cells and tissues at video rate by enhancing the weak Raman signal through nonlinear excitation. Edited by pioneers in the field and with contributions from a distinguished team of experts, Coherent Raman Sc