Single Nucleotide Polymorphisms in the Hsp30 Gene as Markers to Detect Oncohynchus Mykiss (rainbow Trout) and Oncorhynchus Clarkii (cutthroat Trout) Hybridization

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Total Pages : 84 pages
Book Rating : 4.:/5 (921 download)

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Book Synopsis Single Nucleotide Polymorphisms in the Hsp30 Gene as Markers to Detect Oncohynchus Mykiss (rainbow Trout) and Oncorhynchus Clarkii (cutthroat Trout) Hybridization by : Rebecca L. Loda

Download or read book Single Nucleotide Polymorphisms in the Hsp30 Gene as Markers to Detect Oncohynchus Mykiss (rainbow Trout) and Oncorhynchus Clarkii (cutthroat Trout) Hybridization written by Rebecca L. Loda and published by . This book was released on 2004 with total page 84 pages. Available in PDF, EPUB and Kindle. Book excerpt: "Hybridization with introduced rainbow trout has been a formidable barrier to conservation and re-introduction of native species of cutthroat trout in the inter mountain west, including the Truckee River system. To aid in the management of native cutthroat trout, a reliable test needs to be developed to identify and monitor hybrids. Genetic analysis of microsatellites has been employed for hybrid detection, but the high degree of inter- and intra- species variability at these loci limits this approach to specific strains of trout. Because of the amount of genetic variation between subspecies of trout, additional markers are desirable for robust identification of hybrids ... To test the possibility of using genetic variation within the HSP30 locus as markers to detect rainbow trout and cutthroat trout hybrids, DNA from Greenback, Lahontan, Colorado River, Bonneville, Coastal, Yellowstone, Westslope, and Snake River cutthroat trout and four strains of rainbow trout was amplified by PCR and sequenced. Consensus sequences and sequence alignments were produced for each species or subspecies, and the data were analyzed to identify single nucleotide polymorphisms (SNPs) that showed species-specific variation. Four polymorphic loci were identified that allow distinction between rainbow trout and seven of the of the eight cutthroat subspecies. Primers pairs were designed to allow species identification without the need for sequencing, using SNPs in the HSP30 gene for the identification of rainbow/cutthroat hybrids and also hybridization between different strains of cutthroat and cutthroat/rainbow hybrids using high throughput sequencing. We were able to design a primer pair that could distinguish rainbow and cutthroat trout using only PCR. However, additional primers must be developed that can identify cutthroat/rainbow hybrids without sequence analysis."--Abstract.

A Suite of Twelve Single Nucleotide Polymorphism Markers for Detecting Introgression Between Cutthroat and Rainbow Trout

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Total Pages : 4 pages
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Book Synopsis A Suite of Twelve Single Nucleotide Polymorphism Markers for Detecting Introgression Between Cutthroat and Rainbow Trout by : Andrew S. Harwood

Download or read book A Suite of Twelve Single Nucleotide Polymorphism Markers for Detecting Introgression Between Cutthroat and Rainbow Trout written by Andrew S. Harwood and published by . This book was released on 2011 with total page 4 pages. Available in PDF, EPUB and Kindle. Book excerpt: A suite of 12 subspecies and species-specific single nucleotide polymorphism (species-specific SNP) markers was developed to distinguish rainbow trout (RT) Oncorhynchus mykiss from the four major subspecies of cutthroat trout: westslope cutthroat trout (WCT) Oncorhynchus clarki lewisi, Yellowstone cutthroat trout (YCT) Oncorhynchus clarki bouvieri, coastal cutthroat trout (CCT) Oncorhynchus clarki clarki, Lahontan cutthroat trout (LCT) Oncorhynchus clarki henshawi, and their hybrids. Several of the markers were linked to help strengthen hybrid determinations, and sex-specific species-specific SNP assays were also developed.

Diagnostic Single Nucleotide Polymorphisms for Identifying Westslope Cutthroat Trout (Oncorhynchus Clarki Lewisi), Yellowstone Cutthroat Trout (Oncorhynchus Clarkii Bouvieri) and Rainbow Trout (Oncorhynchus Mykiss)

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ISBN 13 :
Total Pages : 5 pages
Book Rating : 4.:/5 (13 download)

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Book Synopsis Diagnostic Single Nucleotide Polymorphisms for Identifying Westslope Cutthroat Trout (Oncorhynchus Clarki Lewisi), Yellowstone Cutthroat Trout (Oncorhynchus Clarkii Bouvieri) and Rainbow Trout (Oncorhynchus Mykiss) by : Steven T. Kalinowski

Download or read book Diagnostic Single Nucleotide Polymorphisms for Identifying Westslope Cutthroat Trout (Oncorhynchus Clarki Lewisi), Yellowstone Cutthroat Trout (Oncorhynchus Clarkii Bouvieri) and Rainbow Trout (Oncorhynchus Mykiss) written by Steven T. Kalinowski and published by . This book was released on 2010 with total page 5 pages. Available in PDF, EPUB and Kindle. Book excerpt: We describe 12 diagnostic single nucleotide polymorphism (SNP) assays for use in species identification among rainbow and cutthroat trout: five of these loci have alleles unique to rainbow trout (Oncorhynchus mykiss), three unique to westslope cutthroat trout (O. clarkii lewisi) and four unique to Yellowstone cutthroat trout (O. clarkii bouvieri). These diagnostic assays were identified using a total of 489 individuals from 26 populations and five fish hatchery strains.

The Hybrid Dilemma

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ISBN 13 :
Total Pages : 66 pages
Book Rating : 4.:/5 (664 download)

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Book Synopsis The Hybrid Dilemma by : Andrew S. Harwood

Download or read book The Hybrid Dilemma written by Andrew S. Harwood and published by . This book was released on 2010 with total page 66 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Development of Nuclear DNA Markers to Detect Hybridization and Assess Phylogenetic Relationships Among Subspecies of Rainbow Trout (Oncorhynchus Mykiss) and Cutthroat Trout (Oncorhynchus Clarki)

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ISBN 13 :
Total Pages : 154 pages
Book Rating : 4.:/5 (454 download)

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Book Synopsis Development of Nuclear DNA Markers to Detect Hybridization and Assess Phylogenetic Relationships Among Subspecies of Rainbow Trout (Oncorhynchus Mykiss) and Cutthroat Trout (Oncorhynchus Clarki) by : J. W. Pearce Smithwick

Download or read book Development of Nuclear DNA Markers to Detect Hybridization and Assess Phylogenetic Relationships Among Subspecies of Rainbow Trout (Oncorhynchus Mykiss) and Cutthroat Trout (Oncorhynchus Clarki) written by J. W. Pearce Smithwick and published by . This book was released on 2000 with total page 154 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Rainbow Trout (Oncorhynchus Mykiss) Invasion and the Spread of Hybridization with Native Westslope Cutthroat Trout (Oncorhynchus Clarkii Lewisi)

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ISBN 13 :
Total Pages : 12 pages
Book Rating : 4.:/5 (13 download)

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Book Synopsis Rainbow Trout (Oncorhynchus Mykiss) Invasion and the Spread of Hybridization with Native Westslope Cutthroat Trout (Oncorhynchus Clarkii Lewisi) by : Matthew C. Boyer

Download or read book Rainbow Trout (Oncorhynchus Mykiss) Invasion and the Spread of Hybridization with Native Westslope Cutthroat Trout (Oncorhynchus Clarkii Lewisi) written by Matthew C. Boyer and published by . This book was released on 2008 with total page 12 pages. Available in PDF, EPUB and Kindle. Book excerpt: We analyzed 13 microsatellite loci to estimate gene flow among westslope cutthroat trout, Oncorhynchus clarkii lewisi, populations and determine the invasion pattern of hybrids between native O. c. lewisi and introduced rainbow trout, Oncorhynchus mykiss, in streams of the upper Flathead River system, Montana (USA) and British Columbia (Canada). Fourteen of 31 sites lacked evidence of O. mykiss introgression, and gene flow among these nonhybridized O. c. lewisi populations was low, as indicated by significant allele frequency divergence among populations (?ST = 0.076, ?ST = 0.094, P

Hybridization Between Yellowstone Cutthroat Trout and Rainbow Trout in the Upper Snake River Basin, Wyoming

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ISBN 13 :
Total Pages : 11 pages
Book Rating : 4.:/5 (13 download)

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Book Synopsis Hybridization Between Yellowstone Cutthroat Trout and Rainbow Trout in the Upper Snake River Basin, Wyoming by : Ryan P. Kovach

Download or read book Hybridization Between Yellowstone Cutthroat Trout and Rainbow Trout in the Upper Snake River Basin, Wyoming written by Ryan P. Kovach and published by . This book was released on 2011 with total page 11 pages. Available in PDF, EPUB and Kindle. Book excerpt: Human-induced hybridization between fish populations and species is a major threat to aquatic biodiversity worldwide and is particularly relevant to management of the subspecies of cutthroat trout Oncorhynchus clarkii. The upper Snake River basin in Wyoming contains one of the largest remaining populations of Yellowstone cutthroat trout O. clarkii bouvieri, a subspecies of special concern throughout its range; however, little is known about levels of hybridization between Yellowstone cutthroat trout and exotic rainbow trout O. mykiss or about the overall genetic population structure for this river basin. There is concern that the Gros Ventre River is a source of hybridization for the Snake River basin. We sampled across the upper Snake River basin to estimate levels of hybridization and population structure and to describe hybrid zone structure and spatial patterns of hybridization throughout the basin. We used this information to help resolve whether the Gros Ventre River was acting as a potential source of hybridization for the upper Snake River basin. We found that Yellowstone cutthroat trout genotypes dominated the river system, but hybridization was detected at low levels in all populations. The Gros Ventre River contained the highest levels of hybridization (population and individual) and displayed evidence of ongoing hybridization between parental genotypes. Levels of hybridization decreased as a function of distance from the Gros Ventre River, suggesting that this population is acting as a source of rainbow trout genes. These patterns were evident despite the fact that levels of genetic connectivity appeared to be higher than those observed in other cutthroat trout populations (global genetic differentiation index F ST = 0.04), and we did not find evidence for genetic isolation by distance. Management actions aimed at reducing the presence of highly hybridized cutthroat trout or rainbow trout individuals in the Gros Ventre River will help to maintain the upper Snake River basin as an important conservation area.

Chromosome Rearrangements, Recombination Suppression, and Limited Segregation Distortion in Hybrids Between Yellowstone Cutthroat Trout (Oncorhynchus Clarkii Bouvieri) and Rainbow Trout (O. Mykiss)

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ISBN 13 :
Total Pages : 16 pages
Book Rating : 4.:/5 (13 download)

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Book Synopsis Chromosome Rearrangements, Recombination Suppression, and Limited Segregation Distortion in Hybrids Between Yellowstone Cutthroat Trout (Oncorhynchus Clarkii Bouvieri) and Rainbow Trout (O. Mykiss) by : Carl O. Ostberg

Download or read book Chromosome Rearrangements, Recombination Suppression, and Limited Segregation Distortion in Hybrids Between Yellowstone Cutthroat Trout (Oncorhynchus Clarkii Bouvieri) and Rainbow Trout (O. Mykiss) written by Carl O. Ostberg and published by . This book was released on 2013 with total page 16 pages. Available in PDF, EPUB and Kindle. Book excerpt: Background Introgressive hybridization is an important evolutionary process that can lead to the creation of novel genome structures and thus potentially new genetic variation for selection to act upon. On the other hand, hybridization with introduced species can threaten native species, such as cutthroat trout (Oncorhynchus clarkii) following the introduction of rainbow trout (O. mykiss). Neither the evolutionary consequences nor conservation implications of rainbow trout introgression in cutthroat trout is well understood. Therefore, we generated a genetic linkage map for rainbow-Yellowstone cutthroat trout (O. clarkii bouvieri) hybrids to evaluate genome processes that may help explain how introgression affects hybrid genome evolution. Results The hybrid map closely aligned with the rainbow trout map (a cutthroat trout map does not exist), sharing all but one linkage group. This linkage group (RYHyb20) represented a fusion between an acrocentric (Omy28) and a metacentric chromosome (Omy20) in rainbow trout. Additional mapping in Yellowstone cutthroat trout indicated the two rainbow trout homologues were fused in the Yellowstone genome. Variation in the number of hybrid linkage groups (28 or 29) likely depended on a Robertsonian rearrangement polymorphism within the rainbow trout stock. Comparison between the female-merged F1 map and a female consensus rainbow trout map revealed that introgression suppressed recombination across large genomic regions in 5 hybrid linkage groups. Two of these linkage groups (RYHyb20 and RYHyb25_29) contained confirmed chromosome rearrangements between rainbow and Yellowstone cutthroat trout indicating that rearrangements may suppress recombination. The frequency of allelic and genotypic segregation distortion varied among parents and families, suggesting few incompatibilities exist between rainbow and Yellowstone cutthroat trout genomes.

Hybridization Between Yellowstone Cutthroat Trout and Rainbow Trout Alters the Expression of Muscle Growth-Related Genes and Their Relationships with Growth Patterns

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ISBN 13 :
Total Pages : 16 pages
Book Rating : 4.:/5 (13 download)

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Book Synopsis Hybridization Between Yellowstone Cutthroat Trout and Rainbow Trout Alters the Expression of Muscle Growth-Related Genes and Their Relationships with Growth Patterns by : Carl O. Ostberg

Download or read book Hybridization Between Yellowstone Cutthroat Trout and Rainbow Trout Alters the Expression of Muscle Growth-Related Genes and Their Relationships with Growth Patterns written by Carl O. Ostberg and published by . This book was released on 2015 with total page 16 pages. Available in PDF, EPUB and Kindle. Book excerpt: Hybridization creates novel gene combinations that may generate important evolutionary novelty, but may also reduce existing adaptation by interrupting inherent biological processes, such as genotype-environment interactions. Hybridization often causes substantial change in patterns of gene expression, which, in turn, may cause phenotypic change. Rainbow trout (Oncorhynchus mykiss) and cutthroat trout (O. clarkii) produce viable hybrids in the wild, and introgressive hybridization with introduced rainbow trout is a major conservation concern for native cutthroat trout. The two species differ in body shape, which is likely an evolutionary adaptation to their native environments, and their hybrids tend to show intermediate morphology. The characterization of gene expression patterns may provide insights on the genetic basis of hybrid and parental morphologies, as well as on the ecological performance of hybrids in the wild. Here, we evaluated the expression of eight growth-related genes (MSTN-1a, MSTN-1b, MyoD1a, MyoD1b, MRF-4, IGF-1, IGF-2, and CAST-L) and the relationship of these genes with growth traits (length, weight, and condition factor) in six line crosses: both parental species, both reciprocal F1 hybrids, and both first-generation backcrosses (F1 x rainbow trout and F1 x cutthroat trout). Four of these genes were differentially expressed among rainbow, cutthroat, and their hybrids. Transcript abundance was significantly correlated with growth traits across the parent species, but not across hybrids. Our findings suggest that rainbow and cutthroat trout exhibit differences in muscle growth regulation, that transcriptional networks may be modified by hybridization, and that hybridization disrupts intrinsic relationships between gene expression and growth patterns that may be functionally important for phenotypic.

PCR-based Sex Specific Markers for Rainbow Trout (Oncorhynchus Mykiss)

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ISBN 13 :
Total Pages : 224 pages
Book Rating : 4.:/5 (54 download)

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Book Synopsis PCR-based Sex Specific Markers for Rainbow Trout (Oncorhynchus Mykiss) by : Alexandre Rodrigues Caetano

Download or read book PCR-based Sex Specific Markers for Rainbow Trout (Oncorhynchus Mykiss) written by Alexandre Rodrigues Caetano and published by . This book was released on 1994 with total page 224 pages. Available in PDF, EPUB and Kindle. Book excerpt: