Single Molecule Tools, Part B: Super-Resolution, Particle Tracking, Multiparameter, and Force Based Methods

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Publisher : Academic Press
ISBN 13 : 0123814839
Total Pages : 745 pages
Book Rating : 4.1/5 (238 download)

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Book Synopsis Single Molecule Tools, Part B: Super-Resolution, Particle Tracking, Multiparameter, and Force Based Methods by :

Download or read book Single Molecule Tools, Part B: Super-Resolution, Particle Tracking, Multiparameter, and Force Based Methods written by and published by Academic Press. This book was released on 2010-07-09 with total page 745 pages. Available in PDF, EPUB and Kindle. Book excerpt: Single molecule tools have begun to revolutionize the molecular sciences, from biophysics to chemistry to cell biology. They hold the promise to be able to directly observe previously unseen molecular heterogeneities, quantitatively dissect complex reaction kinetics, ultimately miniaturize enzyme assays, image components of spatially distributed samples, probe the mechanical properties of single molecules in their native environment, and "just look at the thing" as anticipated by the visionary Richard Feynman already half a century ago. Single Molecule Tools, Part B: Super-Resolution, Particle Tracking, Multiparameter, and Force Based Methods captures a snapshot of this vibrant, rapidly expanding field, presenting articles from pioneers in the field intended to guide both the newcomer and the expert through the intricacies of getting single molecule tools. - Includes time-tested core methods and new innovations applicable to any researcher employing single molecule tools - Methods included are useful to both established researchers and newcomers to the field - Relevant background and reference information given for procedures can be used as a guide to developing protocols in a number of disciplines

Single Molecule Tools

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Publisher :
ISBN 13 :
Total Pages : 663 pages
Book Rating : 4.:/5 (672 download)

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Book Synopsis Single Molecule Tools by :

Download or read book Single Molecule Tools written by and published by . This book was released on 2010 with total page 663 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Methods in Methane Metabolism, Part B

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Publisher : Academic Press
ISBN 13 : 0123869064
Total Pages : 361 pages
Book Rating : 4.1/5 (238 download)

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Book Synopsis Methods in Methane Metabolism, Part B by : Amy Rosenzweig

Download or read book Methods in Methane Metabolism, Part B written by Amy Rosenzweig and published by Academic Press. This book was released on 2011-03-17 with total page 361 pages. Available in PDF, EPUB and Kindle. Book excerpt: Produced by microbes on a large scale, methane is an important alternative fuel as well as a potent greenhouse gas. This volume focuses on microbial methane metabolism, which is central to the global carbon cycle. Both methanotrophy and methanogenesis are covered in detail. Topics include isolation and classification of microorganisms, metagenomics approaches, biochemistry of key metabolic enzymes, gene regulation and genetic systems, and field measurements. The state of the art techniques described here will both guide researchers in specific pursuits and educate the wider scientific community about this exciting and rapidly developing field. - Topics include isolation and classification of microorganisms, metagenomics approaches, biochemistry of key metabolic enzymes, gene regulation and genetic systems and field measurements - The state-of-the-art techniques described here will both guide researchers in specific pursuits and educate the wider scientific community about this exciting and rapidly developing field

Guide to Techniques in Mouse Development, Part B

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Publisher : Academic Press
ISBN 13 : 0123848814
Total Pages : 637 pages
Book Rating : 4.1/5 (238 download)

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Book Synopsis Guide to Techniques in Mouse Development, Part B by : Paul Wassarman

Download or read book Guide to Techniques in Mouse Development, Part B written by Paul Wassarman and published by Academic Press. This book was released on 2010-09-24 with total page 637 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume comprehensively covers new technologies and methodologies that have appeared for the study of mouse development - Update of volume 225, Guide to Techniques in Mouse Development, edited by P.M. Wassarman and M.L. DePamphilis and published in 1993 - Comprehensively covers: - new techniques for the cryopreservation of gametes and embryos - production of transgenic and null (knockout) animals (use of ES cells) - generation of conditional/inducible mutant animals, use of gene-trap mutagenesis - analysis of allele-specific expression, use of new reporter constructs - humanizing of transgenic animals - transcript profiling of mouse development - imaging of mouse development - rederivation of animals and use of mouse genomics

Synthetic Biology, Part B

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Publisher : Academic Press
ISBN 13 : 0123851211
Total Pages : 556 pages
Book Rating : 4.1/5 (238 download)

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Book Synopsis Synthetic Biology, Part B by : Chris Voigt

Download or read book Synthetic Biology, Part B written by Chris Voigt and published by Academic Press. This book was released on 2011-07-15 with total page 556 pages. Available in PDF, EPUB and Kindle. Book excerpt: Synthetic biology encompasses a variety of different approaches, methodologies and disciplines, and many different definitions exist. This Volume of Methods in Enzymology has been split into 2 Parts and covers topics such as Measuring and Engineering Central Dogma Processes, Mathematical and Computational Methods and Next-Generation DNA Assembly and Manipulation. - Encompasses a variety of different approaches, methodologies and disciplines - Split into 2 parts and covers topics such as measuring and engineering central dogma processes, mathematical and computational methods and next-generation DNA assembly and manipulation

Protein Engineering for Therapeutics, Part B

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Publisher : Academic Press
ISBN 13 : 0123972647
Total Pages : 399 pages
Book Rating : 4.1/5 (239 download)

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Book Synopsis Protein Engineering for Therapeutics, Part B by :

Download or read book Protein Engineering for Therapeutics, Part B written by and published by Academic Press. This book was released on 2012-01-25 with total page 399 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume of Methods in Enzymology looks at Protein Engineering for Therapeutics. The chapters provide an invaluable resource for academics, researchers and students alike. With an international board of authors, this volume is split into sections that cover subjects such as Peptides, and Scaffolds - Chapters provide an invaluable resource for academics, researchers and students alike - Iinternational board of authors - This volume is split into sections that cover subjects such as peptides, and scaffolds

Cryo-EM Part B: 3-D Reconstruction

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Publisher : Academic Press
ISBN 13 : 0123849926
Total Pages : 472 pages
Book Rating : 4.1/5 (238 download)

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Book Synopsis Cryo-EM Part B: 3-D Reconstruction by :

Download or read book Cryo-EM Part B: 3-D Reconstruction written by and published by Academic Press. This book was released on 2010-09-30 with total page 472 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume is dedicated to a description of the instruments, samples, protocols, and analyses that belong to cryo-EM. It emphasizes the relatedness of the ideas, instrumentation, and methods underlying all cryo-EM approaches, which allow practitioners to easily move between them. Within each section, the articles are ordered according to the most common symmetry of the sample to which their methods are applied. - Includes time-tested core methods and new innovations applicable to any researcher - Methods included are useful to both established researchers and newcomers to the field - Relevant background and reference information given for procedures can be used as a guide

Research on Nitrification and Related Processes, Part B

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Publisher : Academic Press
ISBN 13 : 0123864909
Total Pages : 583 pages
Book Rating : 4.1/5 (238 download)

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Book Synopsis Research on Nitrification and Related Processes, Part B by :

Download or read book Research on Nitrification and Related Processes, Part B written by and published by Academic Press. This book was released on 2011-06-15 with total page 583 pages. Available in PDF, EPUB and Kindle. Book excerpt: The global nitrogen cycle is the one most impacted by mankind. The past decade has changed our view on many aspects of the microbial biogeochemical cycles, including the global nitrogen cycle, which is mainly due to tremendous advances in methods, techniques and approaches. Many novel processes and the molecular inventory and organisms that facilitate them have been discovered only within the last 5 to 10 years, and the process is in progress. Research on Nitrification and Related Processes, Part B provides state-of-the-art updates on methods and protocols dealing with the detection, isolation and characterization of macromolecules and their hosting organisms that facilitate nitrification and related processes in the nitrogen cycle as well as the challenges of doing so in very diverse environments. - Provides state-of-the-art update on methods and protocols - Deals with the detection, isolation and characterization of macromolecules and their hosting organisms - Deals with the challenges of very diverse environments

Constitutive Activity in Receptors and Other Proteins, Part B

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Publisher : Academic Press
ISBN 13 : 0123812976
Total Pages : 721 pages
Book Rating : 4.1/5 (238 download)

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Book Synopsis Constitutive Activity in Receptors and Other Proteins, Part B by :

Download or read book Constitutive Activity in Receptors and Other Proteins, Part B written by and published by Academic Press. This book was released on 2010-11-24 with total page 721 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume of Methods in Enzymology covers the current methodology for the detection and assessment of constitutively active proteins. The chapters written by expert authors who are leaders in the field, provide hints and tricks not available in primary research publications.It is extensively referenced, with useful figures and tables throughout the volume. - Expert authors who are leaders in the field - Extensively referenced and useful figures and tables - Provides hints and tricks to facilitate reproduction of methods

The Unfolded Protein Response and Cellular Stress, Part B

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Publisher : Academic Press
ISBN 13 : 0123851157
Total Pages : 441 pages
Book Rating : 4.1/5 (238 download)

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Book Synopsis The Unfolded Protein Response and Cellular Stress, Part B by : P. Michael Conn

Download or read book The Unfolded Protein Response and Cellular Stress, Part B written by P. Michael Conn and published by Academic Press. This book was released on 2011-01-22 with total page 441 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume provides descriptions of the occurrence of the UPR, methods used to assess it, pharmacological tools and other methodological approaches to analyze its impact on cellular regulation. The authors explain how these methods are able to provide important biological insights. - This volume provides descriptions of the occurrence of the UPR, methods used to assess it, pharmacological tools and other methodological approaches to analyze its impact on cellular regulation - The authors explain how these methods are able to provide important biological insights

Fragment Based Drug Design

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Publisher : Academic Press
ISBN 13 : 0123812747
Total Pages : 662 pages
Book Rating : 4.1/5 (238 download)

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Book Synopsis Fragment Based Drug Design by : Lawrence C. Kuo

Download or read book Fragment Based Drug Design written by Lawrence C. Kuo and published by Academic Press. This book was released on 2011-03-09 with total page 662 pages. Available in PDF, EPUB and Kindle. Book excerpt: There are numerous excellent reviews on fragment-based drug discovery (FBDD), but there are to date no hand-holding guides or protocols with which one can embark on this orthogonal approach to complement traditional high throughput screening methodologies. This Methods in Enzymology volume offers the tools, practical approaches, and hit-to-lead examples on how to conduct FBDD screens. The chapters in this volume cover methods that have proven to be successful in generating leads from fragments, including chapters on how to apply computational techniques, nuclear magnetic resonance, surface plasma resonance, thermal shift and binding assays, protein crystallography, and medicinal chemistry in FBDD. Also elaborated by experienced researchers in FBDD are sample preparations of fragments, proteins, and GPCR as well as examples of how to generate leads from hits. Offers the tools, practical approaches, and hit-to-lead examples on how to conduct FBDD screens The chapters in this volume cover methods that have proven to be successful in generating leads from fragments, including chapters on how to apply computational techniques, nuclear magnetic resonance, surface plasma resonance, thermal shift and binding assays, protein crystallography, and medicinal chemistry in FBDD

Computer Methods, Part C

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Publisher : Academic Press
ISBN 13 : 0123812712
Total Pages : 701 pages
Book Rating : 4.1/5 (238 download)

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Book Synopsis Computer Methods, Part C by :

Download or read book Computer Methods, Part C written by and published by Academic Press. This book was released on 2010-12-24 with total page 701 pages. Available in PDF, EPUB and Kindle. Book excerpt: The combination of faster, more advanced computers and more quantitatively oriented biomedical researchers has recently yielded new and more precise methods for the analysis of biomedical data. These better analyses have enhanced the conclusions that can be drawn from biomedical data, and they have changed the way that experiments are designed and performed. This volume, along with the 2 previous Computer Methods volumes for the Methods in Enzymology serial, aims to inform biomedical researchers about recent applications of modern data analysis and simulation methods as applied to biomedical research. - Presents step-by-step computer methods and discusses the techniques in detail to enable their implementation in solving a wide range of problems - Informs biomedical researchers of the modern data analysis methods that have developed alongside computer hardware - Presents methods at the "nuts and bolts" level to identify and resolve a problem and analyze what the results mean

Methods in Methane Metabolism, Part A

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Publisher : Academic Press
ISBN 13 : 0123851130
Total Pages : 420 pages
Book Rating : 4.1/5 (238 download)

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Book Synopsis Methods in Methane Metabolism, Part A by : Amy Rosenzweig

Download or read book Methods in Methane Metabolism, Part A written by Amy Rosenzweig and published by Academic Press. This book was released on 2011-05-11 with total page 420 pages. Available in PDF, EPUB and Kindle. Book excerpt: Produced by microbes on a large scale, methane is an important alternative fuel as well as a potent greenhouse gas. This volume focuses on microbial methane metabolism, which is central to the global carbon cycle. Both methanotrophy and methanogenesis are covered in detail. Topics include isolation and classification of microorganisms, metagenomics approaches, biochemistry of key metabolic enzymes, gene regulation and genetic systems, and field measurements. The state-of-the-art techniques described here will both guide researchers in specific pursuits and educate the wider scientific community about this exciting and rapidly developing field. - Topics include isolation and classification of microorganisms, metagenomics approaches, biochemistry of key metabolic enzymes, gene regulation and genetic systems, and field measurements - The state-of-the-art techniques described here will both guide researchers in specific pursuits and educate the wider scientific community about this exciting and rapidly developing field

Methods in Systems Biology

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Publisher : Academic Press
ISBN 13 : 0123851181
Total Pages : 775 pages
Book Rating : 4.1/5 (238 download)

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Book Synopsis Methods in Systems Biology by : Daniel Jameson

Download or read book Methods in Systems Biology written by Daniel Jameson and published by Academic Press. This book was released on 2011-09-26 with total page 775 pages. Available in PDF, EPUB and Kindle. Book excerpt: Systems biology is a term used to describe a number of trends in bioscience research and a movement that draws on those trends. This volume in the Methods in Enzymology series comprehensively covers the methods in systems biology. With an international board of authors, this volume is split into sections that cover subjects such as machines for systems biology, protein production and quantification for systems biology, and enzymatic assays in systems biology research. This volume in the Methods in Enzymology series comprehensively covers the methods in systems biology With an international board of authors, this volume is split into sections that cover subjects such as machines for systems biology, protein production and quantification for systems biology, and enzymatic assays in systems biology research

Methods in Methane Metabolism

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Publisher : Academic Press
ISBN 13 : 0123851122
Total Pages : 421 pages
Book Rating : 4.1/5 (238 download)

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Book Synopsis Methods in Methane Metabolism by : Amy Claire Rosenzweig

Download or read book Methods in Methane Metabolism written by Amy Claire Rosenzweig and published by Academic Press. This book was released on 2011 with total page 421 pages. Available in PDF, EPUB and Kindle. Book excerpt: Produced by microbes on a large scale, methane is an important alternative fuel as well as a potent greenhouse gas. This volume focuses on microbial methane metabolism, which is central to the global carbon cycle. Both methanotrophy and methanogenesis are covered in detail. Topics include isolation and classification of microorganisms, metagenomics approaches, biochemistry of key metabolic enzymes, gene regulation and genetic systems, and field measurements. The state of the art techniques described here will both guide researchers in specific pursuits and educate the wider scientific community about this exciting and rapidly developing field. Topics include isolation and classification of microorganisms, metagenomics approaches, biochemistry of key metabolic enzymes, gene regulation and genetic systems, and field measurements. The state of the art techniques described here will both guide researchers in specific pursuits and educate the wider scientific community about this exciting and rapidly developing field.

Guide to Techniques in Mouse Development, Part A

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Author :
Publisher : Academic Press
ISBN 13 : 0080887783
Total Pages : 539 pages
Book Rating : 4.0/5 (88 download)

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Book Synopsis Guide to Techniques in Mouse Development, Part A by :

Download or read book Guide to Techniques in Mouse Development, Part A written by and published by Academic Press. This book was released on 2010-08-04 with total page 539 pages. Available in PDF, EPUB and Kindle. Book excerpt: Guide to Techniques in Mouse Development, Part A comprehensively covers new technologies and methodologies that have appeared for the study of mouse development. - Update of volume 225 of Methods in Enzymology, Guide to Techniques in Mouse Development, edited by P.M. Wassarman and M.L. DePamphilis and published in 1993 - Covers new technologies and methodologies, including: - new techniques for the cryopreservation of gametes and embryos - production of transgenic and null (knockout) animals (use of ES cells) - generation of conditional/inducible mutant animals - use of gene-trap mutagenesis - analysis of allele-specific expresion - use of new reporter constructs - humanizing of transgenic animals - transcript profiling of mouse development - imaging of mouse development - rederivation of animals and use of mouse genomics

Synthetic Biology, Part A

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Author :
Publisher : Academic Press
ISBN 13 : 0123850762
Total Pages : 733 pages
Book Rating : 4.1/5 (238 download)

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Book Synopsis Synthetic Biology, Part A by : Chris Voigt

Download or read book Synthetic Biology, Part A written by Chris Voigt and published by Academic Press. This book was released on 2011-07-08 with total page 733 pages. Available in PDF, EPUB and Kindle. Book excerpt: Synthetic biology encompasses a variety of different approaches, methodologies and disciplines, and many different definitions exist. This Volume of Methods in Enzymology has been split into 2 Parts and covers topics such as Measuring and Engineering Central Dogma Processes, Mathematical and Computational Methods and Next-Generation DNA Assembly and Manipulation. - Encompasses a variety of different approaches, methodologies and disciplines - Split into 2 parts and covers topics such as measuring and engineering central dogma processes, mathematical and computational methods and next-generation DNA assembly and manipulation