Single Molecule Microscopy In Neurobiology

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Single Molecule Microscopy in Neurobiology

Author : Nobuhiko Yamamoto
Publisher : Humana
ISBN 13 : 9781071605318
Total Pages : 0 pages
Book Rating : 4.6/5 (53 download)

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Book Synopsis Single Molecule Microscopy in Neurobiology by : Nobuhiko Yamamoto

Download or read book Single Molecule Microscopy in Neurobiology written by Nobuhiko Yamamoto and published by Humana. This book was released on 2020-04-29 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume looks at the methodology and techniques used by experts to study how certain molecules function in specific locations, and their temporal patterns. Chapters in this book cover topics such as in vivo single-molecule tracking of voltage-gated calcium channels with split-fluorescent proteins in CRISPR-engineering C. elegans; protein-protein interactions in membranes using single particle tracking; neuropathological diseases revealed by quantum-dot single particle tracking; SPoD-OnSPAN; and investigating molecular diffusion inside small neuronal compartments with two-photon fluorescence correlation spectroscopy. In the Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your laboratory. Cutting-edge and comprehensive, Single Molecule Microscopy is a valuable resource for any researcher interested in learning more about this important field.

Cellular Imaging Techniques for Neuroscience and Beyond

Author : Floris G. Wouterlood
Publisher : Academic Press
ISBN 13 : 0123858720
Total Pages : 298 pages
Book Rating : 4.1/5 (238 download)

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Book Synopsis Cellular Imaging Techniques for Neuroscience and Beyond by : Floris G. Wouterlood

Download or read book Cellular Imaging Techniques for Neuroscience and Beyond written by Floris G. Wouterlood and published by Academic Press. This book was released on 2012-08-08 with total page 298 pages. Available in PDF, EPUB and Kindle. Book excerpt: In the biomedical sciences, the confocal laser scanning microscope (CLSM) has become the instrument of choice for producing high-resolution images and 3D reconstruction, breaking the barriers of conventional optical microscopy. Wouterlood (anatomy, VU U. Medical Center, Amsterdam, the Netherlands) introduces the confocal principle which eliminates out-of-focus haze, its components, and relevant equations. International scientists explain the principles and related methods of stimulated emission depletion (SRED), single molecule localization, and coherent anti-Stokes Raman (CARS) microscopy; labeling approaches; preparation of samples for imaging; and applications of, and developments in, this new wave of imaging, e.g., visualization of neuronal networks, DNA, and myelin. The text includes color and b&w images, and referral to an online CLSM simulator. Academic Press is an imprint of Elsevier. Annotation ©2013 Book News, Inc., Portland, OR (booknews.com).

Imaging in Neuroscience and Development

Author : Rafael Yuste
Publisher : CSHL Press
ISBN 13 : 0879696923
Total Pages : 813 pages
Book Rating : 4.8/5 (796 download)

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Book Synopsis Imaging in Neuroscience and Development by : Rafael Yuste

Download or read book Imaging in Neuroscience and Development written by Rafael Yuste and published by CSHL Press. This book was released on 2005 with total page 813 pages. Available in PDF, EPUB and Kindle. Book excerpt: As imaging studies have continued to expand in scope and sophistication, this new edition of the highly successful and wellâ€“received Imaging Neurons: A Laboratory Manualhas expanded to include development, with over twenty new chapters on such topics as MRI microscopy, imaging early developmental events, and labeling single neurons. Chapters on FRET, FCS/ICS, FRAP, hyperresolution microscopy, single molecule imaging, imaging with quantum dots, and imaging gene expression are included. With over forty full chapters, the manual also includes over forty sections of protocols for imaging techniques.

Quantitative and Qualitative Microscopy

Author : P. Michael Conn
Publisher : Academic Press
ISBN 13 : 1483268179
Total Pages : 511 pages
Book Rating : 4.4/5 (832 download)

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Book Synopsis Quantitative and Qualitative Microscopy by : P. Michael Conn

Download or read book Quantitative and Qualitative Microscopy written by P. Michael Conn and published by Academic Press. This book was released on 2013-10-22 with total page 511 pages. Available in PDF, EPUB and Kindle. Book excerpt: Methods in Neurosciences, Volume 3: Quantitative and Qualitative Microscopy is a collection of papers that deals with microscopic techniques in statistical measures. This volume describes microscopy using sophisticated stains and dyes to advance observation of tests and experiments. Section I describes autoradiography including micro chemical methods, high-resolution autoradiography, and single- or double-label quantitative autoradiography for use in imaging of brain activity patterns or determining cerebral physiology. Section II discusses the quantification of structures through statistical and computational methods including dynamic video imaging technology. Section III explains the use of tracers, toxins, or dyes in tracing neuronal connections. One paper addresses the use of small injections of axonally transported fluorescent tracers. Section IV explains staining technology such as using the silver impregnation method for frozen sections of human nervous tissue that are gathered from tissues preserved in formalin. Section V addresses freezing techniques and those using freeze-fracture methods in neurobiology. The text also discusses cryoprotection and other freezing methods to control ice crystals found in fixed or unfixed brain tissues. Section VI presents the combined and high-resolution methods in polarization microscopy and microscopic investigations. Cellular biologists, micro-chemists, and scientific researchers in the field of micro- and cellular biology will appreciate this book.

Molecular Neurobiology

Author :
Publisher :
ISBN 13 :
Total Pages : 390 pages
Book Rating : 4.3/5 (121 download)

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Book Synopsis Molecular Neurobiology by :

Download or read book Molecular Neurobiology written by and published by . This book was released on 1989 with total page 390 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Nanoscale Imaging of Synapses

Author : U. Valentin Nägerl
Publisher : Humana
ISBN 13 : 9781493949182
Total Pages : 0 pages
Book Rating : 4.9/5 (491 download)

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Book Synopsis Nanoscale Imaging of Synapses by : U. Valentin Nägerl

Download or read book Nanoscale Imaging of Synapses written by U. Valentin Nägerl and published by Humana. This book was released on 2016-08-23 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Synapses underlie rapid and flexible neural communication in the brain and they hold the key to understanding higher brain functions in health and disease. Because they are very small and highly dynamic, it is very difficult to study them with traditional techniques. Fortunately, recent ground-breaking advances in optical microscopy (e.g. STED, PALM, STORM, SIM) have greatly improved our ability to image living synapses at the nanoscale, even down to the level of single molecules. The proposed volume brings together leading researchers to review these exciting new techniques and their application in neurobiological research. It will explain and discuss the basic principles behind the various superresolution modalities, how they are implemented, what their scope and limitations are etc. In addition, several key research discoveries on synapses enabled by these novel approaches will be highlighted.

Imaging Neurons

Author : Rafael Yuste
Publisher :
ISBN 13 :
Total Pages : 848 pages
Book Rating : 4.3/5 (91 download)

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Book Synopsis Imaging Neurons by : Rafael Yuste

Download or read book Imaging Neurons written by Rafael Yuste and published by . This book was released on 2000 with total page 848 pages. Available in PDF, EPUB and Kindle. Book excerpt: In the past decade, advances in microscopy have been coupled with new methods of culturing and labeling cells to generate the new science of imaging. Imaging technologies allow investigators to look directly inside living cells and probe their form and function in unprecedented detail. This approach is revolutionizing many aspects of biomedical research, particularly neuroscience, in which visual techniques have traditionally been so important. This manual is the first comprehensive description of the range of imaging technologies being applied to living cells. With its origins in a laboratory course taught at Cold Spring Harbor Laboratory by the editors and contributors, it is packed with the kind of technical detail and practical advice that are essential for success, yet seldom found in the research literature. It covers both established methods and cutting-edge techniques such as multiphoton excitation microscopy and imaging of genetically engineered probes. Although it is neurons to which these technologies are most commonly applied, the methods described are readily adaptable to many other cell types. This book will therefore be an invaluable aid to investigators in cell and developmental biology and immunology as well as neuroscience who wish to take advantage of the extraordinary insights into cellular function offered by imaging technologies.

Cell Biological Applications of Confocal Microscopy

Author :
Publisher : Elsevier
ISBN 13 : 008049658X
Total Pages : 521 pages
Book Rating : 4.0/5 (84 download)

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Book Synopsis Cell Biological Applications of Confocal Microscopy by :

Download or read book Cell Biological Applications of Confocal Microscopy written by and published by Elsevier. This book was released on 2003-01-04 with total page 521 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume of the acclaimed Methods in Cell Biology series provides specific examples of applications of confocal microscopy to cell biological problems. It is an essential guide for students and scientists in cell biology, neuroscience, and many other areas of biological and biomedical research, as well as research directors and technical staff of microscopy and imaging facilities. An integrated and up-to-date coverage on the many various techniques and uses of the confocal microscope (CM). Includes detailed protocols accessible to new users Details how to set up and run a "Confocal Microscope Core Facility" Contains over 170 figures

New Techniques in Systems Neuroscience

Author : Adam D. Douglass
Publisher : Springer
ISBN 13 : 3319129139
Total Pages : 309 pages
Book Rating : 4.3/5 (191 download)

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Book Synopsis New Techniques in Systems Neuroscience by : Adam D. Douglass

Download or read book New Techniques in Systems Neuroscience written by Adam D. Douglass and published by Springer. This book was released on 2015-04-13 with total page 309 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume is essential reading for anyone wishing to understand the recent explosion of experimental tools in neuroscience that now make it possible to manipulate, record, and understand neuronal activity within the intact brain, and which are helping us learn how the many neurons that comprise a network act together to control behavior. Leaders in the field discuss the latest developments in optogenetics, functional imaging, circuit mapping, and the application of these tools to complex biological problems.

Scanning Electron Microscopy of Cerebellar Cortex

Author : Orlando Castejón
Publisher : Springer Science & Business Media
ISBN 13 : 1461501598
Total Pages : 140 pages
Book Rating : 4.4/5 (615 download)

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Book Synopsis Scanning Electron Microscopy of Cerebellar Cortex by : Orlando Castejón

Download or read book Scanning Electron Microscopy of Cerebellar Cortex written by Orlando Castejón and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 140 pages. Available in PDF, EPUB and Kindle. Book excerpt: In a clear exposition this inspirational book shows the in situ three-dimensional morphology of cerebellar neurons, intracortical circuits and synaptic connections that underpin the functioning of cerebellar neurons in networks. It brings together in one volume a new view of the three-dimensional cytoarchitectonic arrangement of the cerebellar cortex. The book shows the cerebellar extrinsic and intrinsic intracortical circuits formed by mossy and climbing fibers as exposed by the cryofracture methods. The high degree of lateral collateralization of these fibers is also displayed providing new insights on the information processing in the cerebellar cortex. Besides, field emission high resolution electron microscopy shows its potential contribution to the study of synaptic morphology. The concluding chapter deals with the contribution of scanning electron microscopy to cerebellar neurobiology. This monograph is an authoritative survey and a must for anyone who is interested in the structure of the central nervous system. It will also appeal to an interdisciplinary audience who wants to learn more about electron microscopy and neurocytology.

Axon Neurobiology: Fine-Scale Dynamics of Microstructure and Function

Author : Haruyuki Kamiya
Publisher : Frontiers Media SA
ISBN 13 : 2889661873
Total Pages : 272 pages
Book Rating : 4.8/5 (896 download)

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Book Synopsis Axon Neurobiology: Fine-Scale Dynamics of Microstructure and Function by : Haruyuki Kamiya

Download or read book Axon Neurobiology: Fine-Scale Dynamics of Microstructure and Function written by Haruyuki Kamiya and published by Frontiers Media SA. This book was released on 2020-12-09 with total page 272 pages. Available in PDF, EPUB and Kindle. Book excerpt: This eBook is a collection of articles from a Frontiers Research Topic. Frontiers Research Topics are very popular trademarks of the Frontiers Journals Series: they are collections of at least ten articles, all centered on a particular subject. With their unique mix of varied contributions from Original Research to Review Articles, Frontiers Research Topics unify the most influential researchers, the latest key findings and historical advances in a hot research area! Find out more on how to host your own Frontiers Research Topic or contribute to one as an author by contacting the Frontiers Editorial Office: frontiersin.org/about/contact.

Imaging in Neuroscience

Author : Fritjof Helmchen
Publisher :
ISBN 13 : 9780879699376
Total Pages : 0 pages
Book Rating : 4.6/5 (993 download)

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Book Synopsis Imaging in Neuroscience by : Fritjof Helmchen

Download or read book Imaging in Neuroscience written by Fritjof Helmchen and published by . This book was released on 2011 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: As imaging technologies have revolutionized research in many areas of biology and medicine, neuroscientists have often pioneered the use of these new visualization techniques. This volume is an essential guide to discovering and implementing these techniques in the neuroscience lab.

Molecular and Cellular Quantitative Microscopy

Author :
Publisher : Cuvillier Verlag
ISBN 13 : 3736918291
Total Pages : 168 pages
Book Rating : 4.7/5 (369 download)

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Book Synopsis Molecular and Cellular Quantitative Microscopy by :

Download or read book Molecular and Cellular Quantitative Microscopy written by and published by Cuvillier Verlag. This book was released on 2006-04-03 with total page 168 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Handbook of Neurophotonics

Author : Francesco S. Pavone
Publisher : CRC Press
ISBN 13 : 1498718760
Total Pages : 579 pages
Book Rating : 4.4/5 (987 download)

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Book Synopsis Handbook of Neurophotonics by : Francesco S. Pavone

Download or read book Handbook of Neurophotonics written by Francesco S. Pavone and published by CRC Press. This book was released on 2020-05-10 with total page 579 pages. Available in PDF, EPUB and Kindle. Book excerpt: The Handbook of Neurophotonics provides a dedicated overview of neurophotonics, covering the use of advanced optical technologies to record, stimulate, and control the activity of the brain, yielding new insight and advantages over conventional tools due to the adaptability and non-invasive nature of light. Including 32 colour figures, this book addresses functional studies of neurovascular signaling, metabolism, electrical excitation, and hemodynamics, as well as clinical applications for imaging and manipulating brain structure and function. The unifying theme throughout is not only to highlight the technology, but to show how these novel methods are becoming critical to breakthroughs that will lead to advances in our ability to manage and treat human diseases of the brain. Key Features: Provides the first dedicated book on state-of-the-art optical techniques for sensing and imaging across at the cellular, molecular, network, and whole brain levels. Highlights how the methods are used for measurement, control, and tracking of molecular events in live neuronal cells, both in basic research and clinical practice. Covers the entire spectrum of approaches, from optogenetics to functional methods, photostimulation, optical dissection, multiscale imaging, microscopy, and structural imaging. Includes chapters that show use of voltage-sensitive dye imaging, hemodynamic imaging, multiphoton imaging, temporal multiplexing, multiplane microscopy, optoacoustic imaging, near-infrared spectroscopy, and miniature neuroimaging devices to track cortical brain activity.

Single Cell ‘Omics of Neuronal Cells

Author : Jonathan V. Sweedler
Publisher : Springer Nature
ISBN 13 : 107162525X
Total Pages : 302 pages
Book Rating : 4.0/5 (716 download)

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Book Synopsis Single Cell ‘Omics of Neuronal Cells by : Jonathan V. Sweedler

Download or read book Single Cell ‘Omics of Neuronal Cells written by Jonathan V. Sweedler and published by Springer Nature. This book was released on 2022-08-10 with total page 302 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume discusses the latest techniques used in the diverse fields of single cell ‘omics and covers topics such as quantifying the single cell transcriptome; isolation of cells in nanoliter volumes for single cell proteomics measurements by nano-LC-MS/MS; and single cell protein characterization by immunoblotting. A wide range of methodologies are highlighted, ranging from high-yield chemical amplification to mass spectrometry and nanotechnology for the analysis of the chemical constituents of cells. In the Neuromethods series style, chapters include both in-depth overviews, as well as detailed protocols that provide the key advice from specialists you need to get successful results in your laboratory. Cutting-edge and comprehensive, Single Cell ‘Omics of Neuronal Cells is a valuable resource for experienced and novice researchers interested in learning more about this field and its future developments.

Far-Field Optical Nanoscopy

Author : Philip Tinnefeld
Publisher : Springer
ISBN 13 : 3662455471
Total Pages : 340 pages
Book Rating : 4.6/5 (624 download)

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Book Synopsis Far-Field Optical Nanoscopy by : Philip Tinnefeld

Download or read book Far-Field Optical Nanoscopy written by Philip Tinnefeld and published by Springer. This book was released on 2015-02-07 with total page 340 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book describes developments in the field of super-resolution fluorescence microscopy or nanoscopy. In 11 chapters, distinguished scientists and leaders in their respective fields describe different nanoscopy approaches, various labeling technologies, and concrete applications. The topics covered include the principles and applications of the most popular nanoscopy techniques STED and (f)PALM/STORM, along with advances brought about by fluorescent proteins and organic dyes optimized for fluorescence nanoscopy. Furthermore, the photophysics of fluorescent labels is addressed, specifically for improving their photoswitching capabilities. Important applications are also discussed, such as the tracking and counting of molecules to determine acting forces in cells, and quantitative cellular imaging, respectively, as well as the mapping of chemical reaction centers at the nano-scale. The 2014 Chemistry Nobel Prize® was awarded for the ground-breaking developments of super-resolved fluorescence microscopy. In this book, which was co-edited by one of the prize winners, readers will find the most recent developments in this field.

Three-Dimensional Confocal Microscopy: Volume Investigation of Biological Specimens

Author :
Publisher : Academic Press
ISBN 13 : 0323138969
Total Pages : 550 pages
Book Rating : 4.3/5 (231 download)

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Book Synopsis Three-Dimensional Confocal Microscopy: Volume Investigation of Biological Specimens by :

Download or read book Three-Dimensional Confocal Microscopy: Volume Investigation of Biological Specimens written by and published by Academic Press. This book was released on 2012-12-02 with total page 550 pages. Available in PDF, EPUB and Kindle. Book excerpt: The integration of confocal microscopy and volume investigation has led to an unprecedented ability to examine spatial relationships between cellular structure and function. The goal of this book is to familiarize the reader with these new technologies and to demonstrate their applicability to a wide range of biological and clinical problems. Volume investigation Three-dimensional reconstruction Fluroescent probe design Biological applications of confocal microscopy, including calcium imaging, receptor movement, and diagnostic pathology Confocal data display and analysis Twenty-eight pages of color