Single Molecule Fluorescence Microscopy Studies Of Dna Surface Interactions On Chemically Graded Organosilane Surfaces

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Silane Coupling Agents

Author : Edwin P. Plueddemann
Publisher : Springer Science & Business Media
ISBN 13 : 1489903429
Total Pages : 238 pages
Book Rating : 4.4/5 (899 download)

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Book Synopsis Silane Coupling Agents by : Edwin P. Plueddemann

Download or read book Silane Coupling Agents written by Edwin P. Plueddemann and published by Springer Science & Business Media. This book was released on 2013-11-11 with total page 238 pages. Available in PDF, EPUB and Kindle. Book excerpt: * It has been rumored that a bumble bee has such aerodynamic deficiencies that it should be incapable of flight. Fiberglass-reinforced polymer com posites, similarly, have two (apparently) insurmountable obstacles to per formance: 1) Water can hydrolyze any conceivable bond between organic and inorganic phase, and 2) Stresses across the interface during temperature cycling (resulting from a mismatch in thermal expansion coefficients) may exceed the strength of one of the phases. Organofunctional silanes are hybrid organic-inorganic compounds that are used as coupling agents across the organic-inorganic interface to help overcome these two obstacles to composite performance. One of their functions is to use the hydrolytic action of water under equilibrium condi tions to relieve thermally induced stresses across the interface. If equilib rium conditions can be maintained, the two problems act to cancel each other out. Coupling agents are defined primarily as materials that improve the practical adhesive bond of polymer to mineral. This may involve an increase in true adhesion, but it may also involve improved wetting, rheology, and other handling properties. The coupling agent may also modify the inter phase region to strengthen the organic and inorganic boundary layers.

Handbook of Single Molecule Fluorescence Spectroscopy

Author : Chris Gell
Publisher : OUP Oxford
ISBN 13 : 9780198529422
Total Pages : 278 pages
Book Rating : 4.5/5 (294 download)

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Book Synopsis Handbook of Single Molecule Fluorescence Spectroscopy by : Chris Gell

Download or read book Handbook of Single Molecule Fluorescence Spectroscopy written by Chris Gell and published by OUP Oxford. This book was released on 2006-08-17 with total page 278 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book is aimed at providing a practical introduction to single molecule fluorescence experiments, the analysis of the data, and applications of the techniques to the study of biological structure and function. The techniques have wide applications in biology, and underpin some aspects of nanotechnology and quantum information processing.

Quantitative Imaging in Cell Biology

Author :
Publisher : Academic Press
ISBN 13 : 0124202012
Total Pages : 609 pages
Book Rating : 4.1/5 (242 download)

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Book Synopsis Quantitative Imaging in Cell Biology by :

Download or read book Quantitative Imaging in Cell Biology written by and published by Academic Press. This book was released on 2014-06-25 with total page 609 pages. Available in PDF, EPUB and Kindle. Book excerpt: This new volume, number 123, of Methods in Cell Biology looks at methods for quantitative imaging in cell biology. It covers both theoretical and practical aspects of using optical fluorescence microscopy and image analysis techniques for quantitative applications. The introductory chapters cover fundamental concepts and techniques important for obtaining accurate and precise quantitative data from imaging systems. These chapters address how choice of microscope, fluorophores, and digital detector impact the quality of quantitative data, and include step-by-step protocols for capturing and analyzing quantitative images. Common quantitative applications, including co-localization, ratiometric imaging, and counting molecules, are covered in detail. Practical chapters cover topics critical to getting the most out of your imaging system, from microscope maintenance to creating standardized samples for measuring resolution. Later chapters cover recent advances in quantitative imaging techniques, including super-resolution and light sheet microscopy. With cutting-edge material, this comprehensive collection is intended to guide researchers for years to come. Covers sections on model systems and functional studies, imaging-based approaches and emerging studies Chapters are written by experts in the field Cutting-edge material

Polymeric and Self Assembled Hydrogels

Author : Xian Jun Loh
Publisher : Royal Society of Chemistry
ISBN 13 : 1849735611
Total Pages : 275 pages
Book Rating : 4.8/5 (497 download)

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Book Synopsis Polymeric and Self Assembled Hydrogels by : Xian Jun Loh

Download or read book Polymeric and Self Assembled Hydrogels written by Xian Jun Loh and published by Royal Society of Chemistry. This book was released on 2013 with total page 275 pages. Available in PDF, EPUB and Kindle. Book excerpt: The only book to give a complete picture of current hydrogel research, covering all the major applications as well as the fundamental principles behind them.

Current Protocols Essential Laboratory Techniques

Author : Sean R. Gallagher
Publisher : John Wiley & Sons
ISBN 13 : 047094241X
Total Pages : 679 pages
Book Rating : 4.4/5 (79 download)

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Book Synopsis Current Protocols Essential Laboratory Techniques by : Sean R. Gallagher

Download or read book Current Protocols Essential Laboratory Techniques written by Sean R. Gallagher and published by John Wiley & Sons. This book was released on 2012-03-19 with total page 679 pages. Available in PDF, EPUB and Kindle. Book excerpt: The latest title from the acclaimed Current Protocols series, Current Protocols Essential Laboratory Techniques, 2e provides the new researcher with the skills and understanding of the fundamental laboratory procedures necessary to run successful experiments, solve problems, and become a productive member of the modern life science laboratory. From covering the basic skills such as measurement, preparation of reagents and use of basic instrumentation to the more advanced techniques such as blotting, chromatography and real-time PCR, this book will serve as a practical reference manual for any life science researcher. Written by a combination of distinguished investigators and outstanding faculty, Current Protocols Essential Laboratory Techniques, 2e is the cornerstone on which the beginning scientist can develop the skills for a successful research career.

Frontiers in Chemical Engineering

Author : National Research Council
Publisher : National Academies Press
ISBN 13 : 030903793X
Total Pages : 236 pages
Book Rating : 4.3/5 (9 download)

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Book Synopsis Frontiers in Chemical Engineering by : National Research Council

Download or read book Frontiers in Chemical Engineering written by National Research Council and published by National Academies Press. This book was released on 1988-02-01 with total page 236 pages. Available in PDF, EPUB and Kindle. Book excerpt: In the next 10 to 15 years, chemical engineers have the potential to affect every aspect of American life and promote the scientific and industrial leadership of the United States. Frontiers in Chemical Engineering explores the opportunities available and gives a blueprint for turning a multitude of promising visions into realities. It also examines the likely changes in how chemical engineers will be educated and take their place in the profession, and presents new research opportunities.

A Research Agenda for Transforming Separation Science

Author : National Academies of Sciences, Engineering, and Medicine
Publisher : National Academies Press
ISBN 13 : 0309491703
Total Pages : 115 pages
Book Rating : 4.3/5 (94 download)

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Book Synopsis A Research Agenda for Transforming Separation Science by : National Academies of Sciences, Engineering, and Medicine

Download or read book A Research Agenda for Transforming Separation Science written by National Academies of Sciences, Engineering, and Medicine and published by National Academies Press. This book was released on 2019-10-30 with total page 115 pages. Available in PDF, EPUB and Kindle. Book excerpt: Separation science plays a critical role in maintaining our standard of living and quality of life. Many industrial processes and general necessities such as chemicals, medicines, clean water, safe food, and energy sources rely on chemical separations. However, the process of chemical separations is often overlooked during product development and this has led to inefficiency, unnecessary waste, and lack of consensus among chemists and engineers. A reevaluation of system design, establishment of standards, and an increased focus on the advancement of separation science are imperative in supporting increased efficiency, continued U.S. manufacturing competitiveness, and public welfare. A Research Agenda for Transforming Separation Science explores developments in the industry since the 1987 National Academies report, Separation and Purification: Critical Needs and Opportunities. Many needs stated in the original report remain today, in addition to a variety of new challenges due to improved detection limits, advances in medicine, and a recent emphasis on sustainability and environmental stewardship. This report examines emerging chemical separation technologies, relevant developments in intersecting disciplines, and gaps in existing research, and provides recommendations for the application of improved separation science technologies and processes. This research serves as a foundation for transforming separation science, which could reduce global energy use, improve human and environmental health, and advance more efficient practices in various industries.

Single Molecule Spectroscopy

Author : R. Rigler
Publisher : Springer Science & Business Media
ISBN 13 : 3642565441
Total Pages : 375 pages
Book Rating : 4.6/5 (425 download)

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Book Synopsis Single Molecule Spectroscopy by : R. Rigler

Download or read book Single Molecule Spectroscopy written by R. Rigler and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 375 pages. Available in PDF, EPUB and Kindle. Book excerpt: The topics range from single molecule experiments in quantum optics and solid-state physics to analogous investigations in physical chemistry and biophysics.

Silicon Surfaces and Formation of Interfaces

Author : Jarek Dabrowski
Publisher : World Scientific
ISBN 13 : 9789810232863
Total Pages : 580 pages
Book Rating : 4.2/5 (328 download)

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Book Synopsis Silicon Surfaces and Formation of Interfaces by : Jarek Dabrowski

Download or read book Silicon Surfaces and Formation of Interfaces written by Jarek Dabrowski and published by World Scientific. This book was released on 2000 with total page 580 pages. Available in PDF, EPUB and Kindle. Book excerpt: Silicon, the basic material for a multibillion-dollar industry, is the most widely researched and applied semiconductor, and its surfaces are the most thoroughly studied of all semiconductor surfaces. Silicon Surfaces and Formation of Interfaces may be used as an introduction to graduate-level physics and chemical physics. Moreover, it gives a specialized and comprehensive description of the most common faces of silicon crystals as well as their interaction with adsorbates and overlayers. This knowledge is presented in a systematic and easy-to-follow way. Discussion of each system is preceded by a brief overview which categorizes the features and physical mechanisms before the details are presented. The literature is easily available, and the references am numerous and organized in tables, allowing a search without the need to browse through the text. Though this volume focuses on a scientific understanding of physics on the atomistic and mesoscopic levels, it also highlights existing and potential links between basic research in surface science and applications in the silicon industry. It will be valuable to anyone writing a paper, thesis, or proposal in the field of silicon surfaces.

Introduction to Fluorescence

Author : David M. Jameson
Publisher : Taylor & Francis
ISBN 13 : 1439806055
Total Pages : 305 pages
Book Rating : 4.4/5 (398 download)

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Book Synopsis Introduction to Fluorescence by : David M. Jameson

Download or read book Introduction to Fluorescence written by David M. Jameson and published by Taylor & Francis. This book was released on 2014-01-22 with total page 305 pages. Available in PDF, EPUB and Kindle. Book excerpt: In color throughout, this text helps readers acquire a sound understanding of basic fluorescence theory and practice. It takes them through the history of important discoveries to the most current advances. The author introduces the fundamentals of the fluorescence phenomenon and gives detailed examples of fluorescence applications in the molecular life sciences, including biochemistry, biophysics, clinical chemistry and diagnostics, pharmaceutical science, and cell and molecular biology. The text includes references in each chapter, more than 250 figures, and the chemical structures of the most widely used fluorescent molecules.

Spectroscopy and Dynamics of Single Molecules

Author :
Publisher : Elsevier
ISBN 13 : 0128164646
Total Pages : 404 pages
Book Rating : 4.1/5 (281 download)

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Book Synopsis Spectroscopy and Dynamics of Single Molecules by :

Download or read book Spectroscopy and Dynamics of Single Molecules written by and published by Elsevier. This book was released on 2019-08-14 with total page 404 pages. Available in PDF, EPUB and Kindle. Book excerpt: Spectroscopy and Dynamics of Single Molecules: Methods and Applications reviews the most recent developments in spectroscopic methods and applications. Spectroscopic techniques are the chief experimental methods for testing theoretical models and research in this area plays an important role in stimulating new theoretical developments in physical chemistry. This book provides an authoritative insight into the latest advances in the field, highlighting new techniques, current applications, and potential future developments An ideal reference for chemists and physicists alike, Spectroscopy and Dynamics of Single Molecules: Methods and Applications is a useful guide for all those working in the research, design, or application of spectroscopic tools and techniques across a wide range of fields. - Includes the latest research on ultrafast vibrational and electronic dynamics, nonlinear spectroscopies, and single-molecule methods - Makes the content accessible to researchers in chemistry, biophysics, and chemical physics through a rigorous multi-disciplinary approach - Provides content edited by a world-renowned chemist with more than 30 years of experience in research and instruction

Small Molecule Microarrays: Methods and Protocols

Author : Mahesh Uttamchandani
Publisher : Methods in Molecular Biology
ISBN 13 : 9781493982424
Total Pages : 274 pages
Book Rating : 4.9/5 (824 download)

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Book Synopsis Small Molecule Microarrays: Methods and Protocols by : Mahesh Uttamchandani

Download or read book Small Molecule Microarrays: Methods and Protocols written by Mahesh Uttamchandani and published by Methods in Molecular Biology. This book was released on 2018-12-05 with total page 274 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Sample Preparation Techniques in Analytical Chemistry

Author : Somenath Mitra
Publisher : John Wiley & Sons
ISBN 13 : 0471457825
Total Pages : 480 pages
Book Rating : 4.4/5 (714 download)

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Book Synopsis Sample Preparation Techniques in Analytical Chemistry by : Somenath Mitra

Download or read book Sample Preparation Techniques in Analytical Chemistry written by Somenath Mitra and published by John Wiley & Sons. This book was released on 2004-04-07 with total page 480 pages. Available in PDF, EPUB and Kindle. Book excerpt: The importance of accurate sample preparation techniques cannot be overstated--meticulous sample preparation is essential. Often overlooked, it is the midway point where the analytes from the sample matrix are transformed so they are suitable for analysis. Even the best analytical techniques cannot rectify problems generated by sloppy sample pretreatment. Devoted entirely to teaching and reinforcing these necessary pretreatment steps, Sample Preparation Techniques in Analytical Chemistry addresses diverse aspects of this important measurement step. These include: * State-of-the-art extraction techniques for organic and inorganic analytes * Sample preparation in biological measurements * Sample pretreatment in microscopy * Surface enhancement as a sample preparation tool in Raman and IR spectroscopy * Sample concentration and clean-up methods * Quality control steps Designed to serve as a text in an undergraduate or graduate level curriculum, Sample Preparation Techniques in Analytical Chemistry also provides an invaluable reference tool for analytical chemists in the chemical, biological, pharmaceutical, environmental, and materials sciences.

Proteins

Author : Thomas E. Creighton
Publisher : Macmillan
ISBN 13 : 9780716770305
Total Pages : 534 pages
Book Rating : 4.7/5 (73 download)

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Book Synopsis Proteins by : Thomas E. Creighton

Download or read book Proteins written by Thomas E. Creighton and published by Macmillan. This book was released on 1993 with total page 534 pages. Available in PDF, EPUB and Kindle. Book excerpt: Organized on a combined basis of chronology and of structural and functional hierarchy, This comprehensive text describes all aspects of proteins--biosynthesis, evolution, dynamics, ligand binding, catalysis, and energy transduction--not just their structures. This edition (first was 1984) is thoroughly updated--especially in the area of protein biosynthesis--and features end-of-chapter exercises and problems, many of which require the student to consult the cited literature in order to obtain the answer. Annotation copyright by Book News, Inc., Portland, OR

DNA-Protein Interactions

Author : Andrew Arthur Travers
Publisher : Springer Science & Business Media
ISBN 13 : 9780412259906
Total Pages : 194 pages
Book Rating : 4.2/5 (599 download)

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Book Synopsis DNA-Protein Interactions by : Andrew Arthur Travers

Download or read book DNA-Protein Interactions written by Andrew Arthur Travers and published by Springer Science & Business Media. This book was released on 1993-04-30 with total page 194 pages. Available in PDF, EPUB and Kindle. Book excerpt: The binding of proteins to DNA and the manipulation of DNA by proteins are crucial aspects of the biological role of DNA in the living cell. This book provides a comprehensive and lucid discussion of the molecular interactions involved.

Introduction to Nanoscience

Author : Stuart Lindsay
Publisher : OUP Oxford
ISBN 13 : 0191609277
Total Pages : 480 pages
Book Rating : 4.1/5 (916 download)

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Book Synopsis Introduction to Nanoscience by : Stuart Lindsay

Download or read book Introduction to Nanoscience written by Stuart Lindsay and published by OUP Oxford. This book was released on 2009-10-22 with total page 480 pages. Available in PDF, EPUB and Kindle. Book excerpt: Nanoscience is not physics, chemistry, engineering or biology. It is all of them, and it is time for a text that integrates the disciplines. This is such a text, aimed at advanced undergraduates and beginning graduate students in the sciences. The consequences of smallness and quantum behaviour are well known and described Richard Feynman's visionary essay 'There's Plenty of Room at the Bottom' (which is reproduced in this book). Another, critical, but thus far neglected, aspect of nanoscience is the complexity of nanostructures. Hundreds, thousands or hundreds of thousands of atoms make up systems that are complex enough to show what is fashionably called 'emergent behaviour'. Quite new phenomena arise from rare configurations of the system. Examples are the Kramer's theory of reactions (Chapter 3), the Marcus theory of electron transfer (Chapter 8), and enzyme catalysis, molecular motors, and fluctuations in gene expression and splicing, all covered in the final Chapter on Nanobiology. The book is divided into three parts. Part I (The Basics) is a self-contained introduction to quantum mechanics, statistical mechanics and chemical kinetics, calling on no more than basic college calculus. A conceptual approach and an array of examples and conceptual problems will allow even those without the mathematical tools to grasp much of what is important. Part II (The Tools) covers microscopy, single molecule manipulation and measurement, nanofabrication and self-assembly. Part III (Applications) covers electrons in nanostructures, molecular electronics, nano-materials and nanobiology. Each chapter starts with a survey of the required basics, but ends by making contact with current research literature.

Microscale Surface Tension and Its Applications

Author : Pierre Lambert
Publisher : MDPI
ISBN 13 : 3039215647
Total Pages : 240 pages
Book Rating : 4.0/5 (392 download)

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Book Synopsis Microscale Surface Tension and Its Applications by : Pierre Lambert

Download or read book Microscale Surface Tension and Its Applications written by Pierre Lambert and published by MDPI. This book was released on 2019-10-21 with total page 240 pages. Available in PDF, EPUB and Kindle. Book excerpt: Building on advances in miniaturization and soft matter, surface tension effects are a major key to the development of soft/fluidic microrobotics. Benefiting from scaling laws, surface tension and capillary effects can enable sensing, actuation, adhesion, confinement, compliance, and other structural and functional properties necessary in micro- and nanosystems. Various applications are under development: microfluidic and lab-on-chip devices, soft gripping and manipulation of particles, colloidal and interfacial assemblies, fluidic/droplet mechatronics. The capillary action is ubiquitous in drops, bubbles and menisci, opening a broad spectrum of technological solutions and scientific investigations. Identified grand challenges to the establishment of fluidic microrobotics include mastering the dynamics of capillary effects, controlling the hysteresis arising from wetting and evaporation, improving the dispensing and handling of tiny droplets, and developing a mechatronic approach for the control and programming of surface tension effects. In this Special Issue of Micromachines, we invite contributions covering all aspects of microscale engineering relying on surface tension. Particularly, we welcome contributions on fundamentals or applications related to: Drop-botics: fluidic or surface tension-based micro/nanorobotics: capillary manipulation, gripping, and actuation, sensing, folding, propulsion and bio-inspired solutions; Control of surface tension effects: surface tension gradients, active surfactants, thermocapillarity, electrowetting, elastocapillarity; Handling of droplets, bubbles and liquid bridges: dispensing, confinement, displacement, stretching, rupture, evaporation; Capillary forces: modelling, measurement, simulation; Interfacial engineering: smart liquids, surface treatments; Interfacial fluidic and capillary assembly of colloids and devices; Biological applications of surface tension, including lab-on-chip and organ-on-chip systems.