Single Molecule Fluorescence Methods For Dna Nanostructure Assembly And Actuation

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Single-molecule Fluorescence Methods for DNA Nanostructure Assembly and Actuation

Author : Casey Platnich
Publisher :
ISBN 13 :
Total Pages : pages
Book Rating : 4.:/5 (129 download)

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Book Synopsis Single-molecule Fluorescence Methods for DNA Nanostructure Assembly and Actuation by : Casey Platnich

Download or read book Single-molecule Fluorescence Methods for DNA Nanostructure Assembly and Actuation written by Casey Platnich and published by . This book was released on 2021 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: "DNA nanotechnology relies on the molecular recognition properties of DNA to produce complex architectures through self-assembly. DNA nanostructures allow scientists to organize functional materials with nanoscale precision, with applications spanning materials science, bioengineering, and physics. In addition to their remarkable performance as frameworks for other species, DNA constructs also possess intrinsic dynamic properties due to the non-covalent interactions that bring them together and show exceptional promise in the areas of controlled, personalized drug delivery and the development of biosensors.Although DNA nanostructures have become increasingly intricate and functional, the development of tools to study them has lagged. Historically, ensemble measurements have been primarily used to analyze the outputs of DNA nanotechnology, averaging the behaviour of billions of molecules within a sample to generate an output. This averaging means that the structure and performance of each individual construct is obscured, concealing malformed structures and masking properties associated with heterogeneity in size, length, and shape in self-assembled samples. For integration into engineered nanomaterials and biomedical devices, it is imperative that the DNA nanomaterials within a sample be structurally identical, with predictable and controllable behaviour. This goal requires the development of new analytical tools for the real-time observation of nanostructure assembly and actuation.This thesis describes the expansion of single-molecule fluorescence methodologies to probe the self-assembly pathways of DNA nanomaterials. These techniques monitor the self-assembly kinetics of surface-grafted DNA constructs, giving rise to a new generation of monodisperse, user-defined structures prepared using fully automated methods. The dynamic nature of these measurements also allows the single-molecule differentiation of the mechanisms of supramolecular polymerization. Exploring the fine-grained detail of self-assembly will enable the actualization of DNA nanostructures in custom delivery and sensing applications. In establishing new strategies to detect the assembly pathways of DNA architectures, this thesis provides a cohesive framework towards understanding hierarchical DNA assemblies at the single-molecule level"--

Advancing DNA Nanotechnology Using Single Molecule Fluorescence Methodologies

Author : Amani Hariri
Publisher :
ISBN 13 :
Total Pages : pages
Book Rating : 4.:/5 (964 download)

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Book Synopsis Advancing DNA Nanotechnology Using Single Molecule Fluorescence Methodologies by : Amani Hariri

Download or read book Advancing DNA Nanotechnology Using Single Molecule Fluorescence Methodologies written by Amani Hariri and published by . This book was released on 2016 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: "By taking it out of its biological role, DNA has served as a robust, reliable and programmable nanofabrication building block for the assembly of a variety of two- and three-dimensional discrete nanometer-sized structures with arbitrary shapes and designs. These molecularly addressable materials could serve as platforms for the site-specific hybridization with nanometer precision. Besides structural complexity, DNA objects evolved to comprise an intrinsic dynamic character allowing them to respond to surrounding stimuli in a predictable manner. Many researchers have previously constructed nanostructures using methods that rely on the spontaneous assembly of DNA in solution, in which case the more complex the assembly design is, the more error-prone the product becomes. This constitutes a major problem for the application of DNA-based structures in dynamic devices such as machines, motors, robots, and computers, where even small errors in assembly can drastically affect performance. Therefore, quantitative tools for analysing structures and dynamics of complex DNA nanostructures are critical. Single molecule fluorescence techniques have been transformative to the field of nanoscience and have played a leading role in providing a mature understanding and an informative feedback on the building blocks and final products of self-assembly. This thesis demonstrates how different single molecule fluorescence methodologies can be utilized to study and advance the structure, dynamics and integrity of DNA-assemblies, specifically those shaped as nanotubes. Conceptually, this research can be divided into four main parts: (i) synthesis optimization, (ii) structural characterization, (iii) monitoring dynamic structures, and (iv) studying structural dynamics. First, a solid-phase synthesis strategy and its visualization through single-molecule spectroscopy was devised to assemble DNA nanotubes in a stepwise fashion, with a full control over their size and sequence pattern. This method paves the way for the production of custom-made DNA nanotubes with fewer structural flaws than the spontaneous-assembly method. Second, single molecule photobleaching and two-color colocalization approaches were uniquely combined to provide a systematic way of assessing the polydispersity, stoichiometry and degree of defectiveness of different DNA nanotubes structures. These approaches will be of significant importance for many research groups synthesizing large supramolecular structures or studying naturally occurring ones, such as protein clusters, amyloids, etc. Third, in situ single molecule immobilization-based fluorescence microscopy was employed to introduce structural changes into DNA nanotubes by dynamically adjusting one or several of the edge lengths between the building blocks using a combination of strand displacement and loops. This is interesting for sensing applications, especially when the analyte produces large scale, detectable structural changes, and also for creating molecular muscles, capable of extension and contraction under external control. Lastly, dynamics and robustness of DNA nanotubes, reconfigured in response to site-specific deletion of DNA strands, were investigated using two color single molecule microscopy. This strategy enables to develop a better understanding of the collective structural changes within DNA structures in response to modifications in their repeat unit. Together, the different methods developed in this thesis underline the importance of single molecule techniques as powerful tools which can advance the field of DNA nanotechnology by enabling the production of well-defined high-quality objects that can meet the designer's compositional and dynamic specifications." --

A Self Assembly Approach to Localization and Patterning of Optically Resolved Single Molecules

Author : Randall Mark Stoltenberg
Publisher : Stanford University
ISBN 13 :
Total Pages : 249 pages
Book Rating : 4.F/5 ( download)

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Book Synopsis A Self Assembly Approach to Localization and Patterning of Optically Resolved Single Molecules by : Randall Mark Stoltenberg

Download or read book A Self Assembly Approach to Localization and Patterning of Optically Resolved Single Molecules written by Randall Mark Stoltenberg and published by Stanford University. This book was released on 2011 with total page 249 pages. Available in PDF, EPUB and Kindle. Book excerpt: Directed assembly of single molecules is a central theme in nanotechnology. This body of work was inspired by a specific challenge involving ordered deposition of single DNAs on surfaces for massively parallel single molecule DNA sequencing via fluorescence microscopy. A potential 10-fold gain in data density is possible if single molecules can be forced into a regular array rather than randomly deposited. The dimensions of such an array are difficult to achieve with conventional lithography techniques. On one end, molecules must be separated by sufficient distance so their optical signatures do not overlap. This distance is on the order of hundreds of nanometers. On the other end, the attachment points for the molecules must have molecular dimensions. Bridging these two length scales is a formidable task. The ability to place nanometer scale objects with nanometer precision can been achieved through atomic force microscopy, scanning tunneling microscopy, optical tweezers, and ebeam lithography. All of these techniques, however, are serial in nature and hence do not serve the intended gain in data density. Another approach toward directed patterning of single molecules is through self-assembly. In this work, self-assembly of block copolymers is explored as a means to addressing the molecular and optical resolution length scales simultaneously. First, the challenge of molecular patterning for single molecule fluorescence microscopy is explored theoretically and the limits of this approach are defined. Block copolymers are introduced as a possible solution to generating the correct surface patterns for improved data density, and experimental results are compared to theoretical predictions. Second, the surface chemistry of these arrays is characterized, and I will show they can be selectively functionalized in preparation for directed assembly of DNAs. Third, these arrays are integrated into single molecule fluorescence imaging experiments to determine their potential for improved data density. What emerges from this work is not only a viable platform for increased single molecule fluorescence data density, but also a deeper understanding of the requirements for directed self-assembly of single molecules.

DNA and RNA Origami

Author : Julián Valero
Publisher : Springer Nature
ISBN 13 : 1071630288
Total Pages : 351 pages
Book Rating : 4.0/5 (716 download)

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Book Synopsis DNA and RNA Origami by : Julián Valero

Download or read book DNA and RNA Origami written by Julián Valero and published by Springer Nature. This book was released on 2023-05-11 with total page 351 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume detials diverse methodological approaches on the assembly and applications of DNA origami assemblies. Chapters guide readers through different synthetic and computational methods, isolation and structural characterization of 2D and 3D DNA origami nanoarchitectures, nanophotonics, drug delivery, biophysics, and synthetic biology.Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, DNA and RNA Origami: Methods and Protocols aims to serve as a guideline describing the current state-of-the-art assembly methodologies and applications of DNA origami nanostructures.

Fluorescence in Bio-inspired Nanotechnology

Author : Jonas Hannestad
Publisher : Springer Science & Business Media
ISBN 13 : 3319010689
Total Pages : 127 pages
Book Rating : 4.3/5 (19 download)

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Book Synopsis Fluorescence in Bio-inspired Nanotechnology by : Jonas Hannestad

Download or read book Fluorescence in Bio-inspired Nanotechnology written by Jonas Hannestad and published by Springer Science & Business Media. This book was released on 2013-07-20 with total page 127 pages. Available in PDF, EPUB and Kindle. Book excerpt: In his thesis Fluorescence in Bio-inspired Nanotechnology, Jonas Hannestad describes the evolving field of DNA nanotechnology in a lucid and easily accessible way. A central theme in the thesis is how biological structures and mechanisms constitute a basis for the design of novel technologies. Hannestad discusses how self-assembled, nanometer-scale DNA constructs can be functionalized using fluorescent labeling. In particular, he highlights how applications are based on fluorescence resonance energy transfer (FRET). Another important contribution is the development of a lipid monolayer platform for the step-by-step assembly of DNA nanoconstructs. The work in the thesis is based on five peer-reviewed papers published in high-profile journals, all of which involve major contributions from the author.

Single Molecule Super-resolution Microscopy Study on the Precision with which DNA Nanostructures Can Orient Fluorescent Dyes

Author : Brett Michael Ward
Publisher :
ISBN 13 :
Total Pages : 0 pages
Book Rating : 4.:/5 (134 download)

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Book Synopsis Single Molecule Super-resolution Microscopy Study on the Precision with which DNA Nanostructures Can Orient Fluorescent Dyes by : Brett Michael Ward

Download or read book Single Molecule Super-resolution Microscopy Study on the Precision with which DNA Nanostructures Can Orient Fluorescent Dyes written by Brett Michael Ward and published by . This book was released on 2020 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: "DNA nanotechnology enables the rapid, programmable self-assembly of novel structures and devices at the nanoscale. Utilizing the simplicity of Watson-Crick base pairing, DNA nanostructures are capable of assembling a variety of nanoparticles in arbitrary configurations with relative ease. Several emerging opto-electronic systems require a high degree of control of both the position and orientation of component fluorescent molecules, and while DNA nanostructures have demonstrated these capabilities, the precision with which DNA can orient fluorescent molecules is not well understood. Determining these bounds is critical in establishing the viability of DNA nanotechnology as a method of assembling fluorescent molecular networks. In this work, using a combination of single molecule emission dipole imaging and super-resolution microscopy techniques, we correlate the orientations of fluorescent dye molecules to the orientations of their DNA substrates along five degrees of freedom. Several species of dyes were embedded within a DNA sequence using either one or two covalent tethers. These strands were incorporated directly into DNA origami structures to investigate the dependence of the location and binding architecture of the dye on the orientational precision of DNA nanostructures. Dye functionalized strands were also folded into a simpler four-arm junction, which was then immobilized on an origami structure to study the influence of the DNA substrate on dye orientation. Correlated analysis of super-resolution images of origami structures and single molecule emission dipole images from the embedded fluorescent molecule within the same structure allowed us to directly measure the relative orientations of dye molecules within DNA nanostructures. The resulting measurements revealed a moderate degree of polar angle control but a large variation in azimuthal control for the majority of structures examined. These measurements establish a single-molecule method for measurement of correlated orientations and provide a powerful approach for future studies on increasing the precision in the orientational control of fluorescent dye molecule monomers by DNA nanostructures."--Boise State University ScholarWorks.

Artificially Controllable Nanodevices Constructed by DNA Origami Technology

Author : Yangyang Yang
Publisher : Springer
ISBN 13 : 9784431566908
Total Pages : 0 pages
Book Rating : 4.5/5 (669 download)

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Book Synopsis Artificially Controllable Nanodevices Constructed by DNA Origami Technology by : Yangyang Yang

Download or read book Artificially Controllable Nanodevices Constructed by DNA Origami Technology written by Yangyang Yang and published by Springer. This book was released on 2018-04-07 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: In this book, the author deals mainly with two topics: (1) single-molecule visualization of switching behaviors in the DNA nanoframe system utilizing different kinds of molecular switches through the use of high-speed atomic force microscope (AFM); (2) construction of photocontrollable DNA nanostructures in programmed patterns and direct visualization of the dynamic assembling process. Here, high-speed AFM was employed to observe the dynamic movements of single molecules. Compared to a traditional single-molecule analysis method, such as fluorescence spectroscopy or electron microscopy, high-speed AFM makes possible the real-time observation of molecule behaviors. DNA nanostructures were designed and assembled as scaffolds to incorporate interested biomolecules. The observations were carried out under robust conditions without complicated pretreatment. Moreover, the photoresponsive molecules were successfully assembled into around 100 nm-sized DNA nanostructures. The assembly/disassembly of nanostructures can be regulated reversibly by photoirradiation. This book explains how DNA origami has gradually become a useful tool for the investigation of biochemical interactions in defined nanospace. It also shows the possibility of DNA nanostructures acting as nanodevices for application in biological systems, serving as a good introduction to basic DNA nanotechnology.

Structural DNA Nanotechnology

Author : Nadrian C. Seeman
Publisher : Cambridge University Press
ISBN 13 : 0521764483
Total Pages : 269 pages
Book Rating : 4.5/5 (217 download)

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Book Synopsis Structural DNA Nanotechnology by : Nadrian C. Seeman

Download or read book Structural DNA Nanotechnology written by Nadrian C. Seeman and published by Cambridge University Press. This book was released on 2015 with total page 269 pages. Available in PDF, EPUB and Kindle. Book excerpt: Written by the founder of the field, this is a comprehensive and accessible introduction to structural DNA nanotechnology.

Developing Single-molecule Fluorescence Techniques and Their Application in the DNA Nanotechnology Field

Author : Roman Tsukanov
Publisher :
ISBN 13 :
Total Pages : 122 pages
Book Rating : 4.:/5 (97 download)

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Book Synopsis Developing Single-molecule Fluorescence Techniques and Their Application in the DNA Nanotechnology Field by : Roman Tsukanov

Download or read book Developing Single-molecule Fluorescence Techniques and Their Application in the DNA Nanotechnology Field written by Roman Tsukanov and published by . This book was released on 2014 with total page 122 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Protein Self-Assembly

Author : Jennifer J. McManus
Publisher : Humana
ISBN 13 : 9781493996803
Total Pages : 266 pages
Book Rating : 4.9/5 (968 download)

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Book Synopsis Protein Self-Assembly by : Jennifer J. McManus

Download or read book Protein Self-Assembly written by Jennifer J. McManus and published by Humana. This book was released on 2020-08-08 with total page 266 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume explores experimental and computational approaches to measuring the most widely studied protein assemblies, including condensed liquid phases, aggregates, and crystals. The chapters in this book are organized into three parts: Part One looks at the techniques used to measure protein-protein interactions and equilibrium protein phases in dilute and concentrated protein solutions; Part Two describes methods to measure kinetics of aggregation and to characterize the assembled state; and Part Three details several different computational approaches that are currently used to help researchers understand protein self-assembly. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Thorough and cutting-edge, Protein Self-Assembly: Methods and Protocols is a valuable resource for researchers who are interested in learning more about this developing field.

Handbook of Single Molecule Fluorescence Spectroscopy

Author : Chris Gell
Publisher : OUP Oxford
ISBN 13 : 0191523852
Total Pages : 278 pages
Book Rating : 4.1/5 (915 download)

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Book Synopsis Handbook of Single Molecule Fluorescence Spectroscopy by : Chris Gell

Download or read book Handbook of Single Molecule Fluorescence Spectroscopy written by Chris Gell and published by OUP Oxford. This book was released on 2006-08-17 with total page 278 pages. Available in PDF, EPUB and Kindle. Book excerpt: Analytical measurements at the single molecule level under ambient conditions have become almost routine in the past few years. The application of this technology to fundamental studies of heterogeneity in biomolecular structure and dynamics, chemical and biological reaction kinetics, and photophysics provides a rich playground for molecular scientists. The potential use of single molecule detection for nanotechnology and quantum information processing is a new and almost unexplored area. This handbook is intended for those interested in a practical introduction to single molecule investigations using fluorescence techniques and places special emphasis on the practicalities of achieving single molecule resolution, analysing the resulting data and exploration of the applications in biophysics. It is ideal for graduate research students and others embarking on work in this exciting field.

Single Molecule Tools, Part A: Fluorescence Based Approaches

Author :
Publisher : Academic Press
ISBN 13 : 008095927X
Total Pages : 559 pages
Book Rating : 4.0/5 (89 download)

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Book Synopsis Single Molecule Tools, Part A: Fluorescence Based Approaches by :

Download or read book Single Molecule Tools, Part A: Fluorescence Based Approaches written by and published by Academic Press. This book was released on 2010-08-17 with total page 559 pages. Available in PDF, EPUB and Kindle. Book excerpt: Single molecule tools have begun to revolutionize the molecular sciences, from biophysics to chemistry to cell biology. They hold the promise to be able to directly observe previously unseen molecular heterogeneities, quantitatively dissect complex reaction kinetics, ultimately miniaturize enzyme assays, image components of spatially distributed samples, probe the mechanical properties of single molecules in their native environment, and "just look at the thing" as anticipated by the visionary Richard Feynman already half a century ago. Single Molecule Tools, Part A: Fluorescence Based Approaches captures a snapshot of this vibrant, rapidly expanding field, presenting articles from pioneers in the field intended to guide both the newcomer and the expert through the intricacies of getting single molecule tools. Includes time-tested core methods and new innovations applicable to any researcher employing single molecule tools Methods included are useful to both established researchers and newcomers to the field Relevant background and reference information given for procedures can be used as a guide to developing protocols in a number of disciplines

Optical Antennas

Author : Mario Agio
Publisher : Cambridge University Press
ISBN 13 : 110701414X
Total Pages : 481 pages
Book Rating : 4.1/5 (7 download)

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Book Synopsis Optical Antennas by : Mario Agio

Download or read book Optical Antennas written by Mario Agio and published by Cambridge University Press. This book was released on 2013-01-03 with total page 481 pages. Available in PDF, EPUB and Kindle. Book excerpt: This consistent and systematic review of recent advances in optical antenna theory and practice brings together leading experts in the fields of electrical engineering, nano-optics and nano-photonics, physical chemistry and nanofabrication. Fundamental concepts and functionalities relevant to optical antennas are explained, together with key principles for optical antenna modelling, design and characterisation. Recognising the tremendous potential of this technology, practical applications are also outlined. Presenting a clear translation of the concepts of radio antenna design, near-field optics and field-enhanced spectroscopy into optical antennas, this interdisciplinary book is an indispensable resource for researchers and graduate students in engineering, optics and photonics, physics and chemistry.

Dna Nanotechnology For Bioanalysis: From Hybrid Dna Nanostructures To Functional Devices

Author : Giuseppe Domenico Arrabito
Publisher : World Scientific
ISBN 13 : 1786343819
Total Pages : 216 pages
Book Rating : 4.7/5 (863 download)

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Book Synopsis Dna Nanotechnology For Bioanalysis: From Hybrid Dna Nanostructures To Functional Devices by : Giuseppe Domenico Arrabito

Download or read book Dna Nanotechnology For Bioanalysis: From Hybrid Dna Nanostructures To Functional Devices written by Giuseppe Domenico Arrabito and published by World Scientific. This book was released on 2017-09-25 with total page 216 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book is intended for non-specialists and students, presenting a unique introduction to the field of DNA nanotechnology. The primary focus is on the extraordinary advantages of specificity and sensitivity obtained by integrating DNA nanostructures in bioanalytical devices.DNA Nanotechnology for Bioanalysis provides a concise and rigorous description for the fabrication of various types of functional nanostructures by optimized software-aided high-yield synthesis. Following this is the explanation of methods to decorate these nanostructures with molecules such as proteins, metal nanoparticles or bioorganic moieties covalently bonded onto DNA via self-assemblage processes. Also provided is a concise review on non-canonical DNA structures (such as G-quadruplexes) and their targeting by small molecules for applications in pharmacology. Finally, it describes the exciting applications of DNA nanostructures in life sciences and nanomedicine, including ultraspecific molecular delivery, control of cell behavior, analysis of cell lysate and DNA-based nano-tools for super-resolution sub-cellular imaging.

A Self Assembly Approach to Localization and Patterning of Optically Resolved Single Molecules

Author : Randall Mark Stoltenberg
Publisher :
ISBN 13 :
Total Pages : pages
Book Rating : 4.:/5 (745 download)

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Book Synopsis A Self Assembly Approach to Localization and Patterning of Optically Resolved Single Molecules by : Randall Mark Stoltenberg

Download or read book A Self Assembly Approach to Localization and Patterning of Optically Resolved Single Molecules written by Randall Mark Stoltenberg and published by . This book was released on 2011 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: Directed assembly of single molecules is a central theme in nanotechnology. This body of work was inspired by a specific challenge involving ordered deposition of single DNAs on surfaces for massively parallel single molecule DNA sequencing via fluorescence microscopy. A potential 10-fold gain in data density is possible if single molecules can be forced into a regular array rather than randomly deposited. The dimensions of such an array are difficult to achieve with conventional lithography techniques. On one end, molecules must be separated by sufficient distance so their optical signatures do not overlap. This distance is on the order of hundreds of nanometers. On the other end, the attachment points for the molecules must have molecular dimensions. Bridging these two length scales is a formidable task. The ability to place nanometer scale objects with nanometer precision can been achieved through atomic force microscopy, scanning tunneling microscopy, optical tweezers, and ebeam lithography. All of these techniques, however, are serial in nature and hence do not serve the intended gain in data density. Another approach toward directed patterning of single molecules is through self-assembly. In this work, self-assembly of block copolymers is explored as a means to addressing the molecular and optical resolution length scales simultaneously. First, the challenge of molecular patterning for single molecule fluorescence microscopy is explored theoretically and the limits of this approach are defined. Block copolymers are introduced as a possible solution to generating the correct surface patterns for improved data density, and experimental results are compared to theoretical predictions. Second, the surface chemistry of these arrays is characterized, and I will show they can be selectively functionalized in preparation for directed assembly of DNAs. Third, these arrays are integrated into single molecule fluorescence imaging experiments to determine their potential for improved data density. What emerges from this work is not only a viable platform for increased single molecule fluorescence data density, but also a deeper understanding of the requirements for directed self-assembly of single molecules.

Campanian Foraminifera from the Stanford University Campus, California

Author : Joseph John Graham
Publisher :
ISBN 13 :
Total Pages : 278 pages
Book Rating : 4.F/5 ( download)

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Book Synopsis Campanian Foraminifera from the Stanford University Campus, California by : Joseph John Graham

Download or read book Campanian Foraminifera from the Stanford University Campus, California written by Joseph John Graham and published by . This book was released on 1963 with total page 278 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Novel Approaches for Single Molecule Activation and Detection

Author : Fabio Benfenati
Publisher : Springer
ISBN 13 : 3662433672
Total Pages : 227 pages
Book Rating : 4.6/5 (624 download)

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Book Synopsis Novel Approaches for Single Molecule Activation and Detection by : Fabio Benfenati

Download or read book Novel Approaches for Single Molecule Activation and Detection written by Fabio Benfenati and published by Springer. This book was released on 2014-06-26 with total page 227 pages. Available in PDF, EPUB and Kindle. Book excerpt: How can we obtain tools able to process and exchange information at the molecular scale? In order to do this, it is necessary to activate and detect single molecules under controlled conditions. This book focuses on the generation of biologically-inspired molecular devices. These devices are based on the developments of new photonic tools able to activate and stimulate single molecule machines. Additionally, new light sensitive molecules can be selectively activated by photonic tools. These technological innovations will provide a way to control activation of single light-sensitive molecules, allowing the investigation of molecular computation in a biological environment.