Separation Of Proteins By Liquid Liquid Extraction In Aqueous Polymeric Two Phase Systems

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Protein Structure Analysis

Author : Roza Maria Kamp
Publisher : Springer Science & Business Media
ISBN 13 : 3642592198
Total Pages : 311 pages
Book Rating : 4.6/5 (425 download)

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Book Synopsis Protein Structure Analysis by : Roza Maria Kamp

Download or read book Protein Structure Analysis written by Roza Maria Kamp and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 311 pages. Available in PDF, EPUB and Kindle. Book excerpt: "Protein Structure Analysis - Preparation and Characterization" is a compilation of practical approaches to the structural analysis of proteins and peptides. Here, about 20 authors describe and comment on techniques for sensitive protein purification and analysis. These methods are used worldwide in biochemical and biotechnical research currently being carried out in pharmaceu tical and biomedical laboratories or protein sequencing facilities. The chapters have been written by scientists with extensive ex perience in these fields, and the practical parts are well documen ted so that the reader should be able to easily reproduce the described techniques. The methods compiled in this book were demonstrated in student courses and in the EMBO Practical Course on "Microsequence Analysis of Proteins" held in Berlin September 10-15, 1995. The topics also derived from a FEBS Workshop, held in Halkidiki, Thessaloniki, Greece, in April, 1995. Most of the authors participated in these courses as lecturers and tutors and made these courses extremely lively and successful. Since polypeptides greatly vary depending on their specific structure and function, strategies for their structural analysis must for the most part be adapted to each individual protein. Therefore, advantages and limitations of the experimen tal approaches are discussed here critically, so that the reader becomes familiar with problems that might be encountered.

Aqueous Two-Phase Systems

Author : Rajni Hatti-Kaul
Publisher : Springer Science & Business Media
ISBN 13 : 1592590284
Total Pages : 442 pages
Book Rating : 4.5/5 (925 download)

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Book Synopsis Aqueous Two-Phase Systems by : Rajni Hatti-Kaul

Download or read book Aqueous Two-Phase Systems written by Rajni Hatti-Kaul and published by Springer Science & Business Media. This book was released on 2008-02-05 with total page 442 pages. Available in PDF, EPUB and Kindle. Book excerpt: A mixture of two polymers, or one polymer and a salt, in an aqueous medium separates into two phases: this phenomenon is useful in biotechn- ogy for product separations. Separation of biological molecules and particles in these aqueous two-phase systems (ATPS) was initiated over 40 years ago by P.-Å. Albertsson, and later proved to be of immense utility in biochemical and cell biological research. A boost in the application of ATPS was seen when problems of separations in biotechnology processes were encountered. Its simplicity, biocompatibility, and amenability to easy scaleup operations make the use of ATPS very attractive for large-scale bioseparations. Despite the advantages ATPS enjoys over other separation techniques, the application of two-phase systems has for a long time been confined to selected labora- ries. Recent years have, however, shown a trend in which increasing numbers of researchers employ two-phase partitioning techniques in both basic and applied research.

Extractive Bioconversions

Author : B. Mattiasson
Publisher : CRC Press
ISBN 13 : 9780824782726
Total Pages : 348 pages
Book Rating : 4.7/5 (827 download)

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Book Synopsis Extractive Bioconversions by : B. Mattiasson

Download or read book Extractive Bioconversions written by B. Mattiasson and published by CRC Press. This book was released on 1990-11-30 with total page 348 pages. Available in PDF, EPUB and Kindle. Book excerpt: Contributors from European and US universities and corporations review recent developments in the integration of downstream processing and bioconversion, describing their experience with many separation technologies, including some still in the experimental stage. The topics include the construction

Ionic-Liquid-Based Aqueous Biphasic Systems

Author : Mara G. Freire
Publisher : Springer
ISBN 13 : 3662528754
Total Pages : 322 pages
Book Rating : 4.6/5 (625 download)

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Book Synopsis Ionic-Liquid-Based Aqueous Biphasic Systems by : Mara G. Freire

Download or read book Ionic-Liquid-Based Aqueous Biphasic Systems written by Mara G. Freire and published by Springer. This book was released on 2016-10-05 with total page 322 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book offers comprehensive information on the fundamentals and applications of ionic-liquid-based aqueous biphasic systems, which have predominantly (and successfully) been employed as alternative platforms for the extraction, separation and purification of diverse high-value products. The book consists of an initial introduction providing a brief overview, from fundamentals to applications, followed by nine chapters addressing the respective phase diagrams (interpretation and characterization) and remarkable examples of their applications. It also includes two final chapters focusing on recent developments in the search for more environmentally-benign and biocompatible ionic-liquid-based aqueous biphasic systems, and on the progress made to date concerning the recovery, recycling and reuse of the phase-forming components, the goal being the development of cost-effective and sustainable processes. The book offers an interesting and useful guide for a broad readership in the fields of green chemistry, biotechnology, chemical engineering, and biochemistry, among others. Mara G. Freire is a Coordinator Researcher at CICECO - Aveiro Institute of Materials, Chemistry Department, University of Aveiro, Portugal.

Liquid Biphasic System

Author : Pau Loke Show
Publisher : Elsevier
ISBN 13 : 0128223987
Total Pages : 330 pages
Book Rating : 4.1/5 (282 download)

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Book Synopsis Liquid Biphasic System by : Pau Loke Show

Download or read book Liquid Biphasic System written by Pau Loke Show and published by Elsevier. This book was released on 2020-11-28 with total page 330 pages. Available in PDF, EPUB and Kindle. Book excerpt: Downstream bioprocesses have a significant role to play in the creation of a sustainable bio-based economy, enabling the creation of new products and systems from the more sustainable bioprocessing of natural products. Liquid Biphasic System: Fundamentals, Methods, and Applications in Bioseparation Technology explores in detail the fundamental processes and applications of this new separation system, aiding understanding of the basic principles of the technique and offering constructive criticisms on the latest findings.Including coverage of the background, principles, mechanisms, and applications, Liquid Biphasic System addresses how to adapt the technology for the purification of useful compounds with greater cost efficiency and greener processing. It is essential reading for bioprocess engineers, biochemical engineers, biosystem engineers, chemists and microbiologists working in the fields of bioprocessing. Researchers, scientists, and engineers concerned with the selection and evaluation of alternative bioseparation processes will find the book particularly useful. - Provides information and examples of advanced separations in a single source - Includes detailed descriptions of novel bioseparation systems - Covers the latest technologies related to advanced liquid–liquid separation and their applications in various industries

Intensification of Biobased Processes

Author : Andrzej Gorak
Publisher : Royal Society of Chemistry
ISBN 13 : 1788010329
Total Pages : 544 pages
Book Rating : 4.7/5 (88 download)

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Book Synopsis Intensification of Biobased Processes by : Andrzej Gorak

Download or read book Intensification of Biobased Processes written by Andrzej Gorak and published by Royal Society of Chemistry. This book was released on 2018-06-18 with total page 544 pages. Available in PDF, EPUB and Kindle. Book excerpt: The first book dedicated entirely to this area, Intensification of Biobased Processes provides a comprehensive overview of modern process intensification technologies used in bioprocessing.

Aqueous Two-Phase Systems for Bioprocess Development for the Recovery of Biological Products

Author : Marco Rito-Palomares
Publisher : Springer
ISBN 13 : 3319593099
Total Pages : 196 pages
Book Rating : 4.3/5 (195 download)

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Book Synopsis Aqueous Two-Phase Systems for Bioprocess Development for the Recovery of Biological Products by : Marco Rito-Palomares

Download or read book Aqueous Two-Phase Systems for Bioprocess Development for the Recovery of Biological Products written by Marco Rito-Palomares and published by Springer. This book was released on 2017-07-18 with total page 196 pages. Available in PDF, EPUB and Kindle. Book excerpt: This comprehensive and unique text presents a full overview of downstream processing useful for those new to the concept as well as professionals with experience in the area. The history and theoretical principles of Aqueous Two-Phase Systems (ATPS) are covered in depth. Information on ATPS characterization and application is included, and ATPS equilibria and system parameters that have significant effect on partition behavior are studied. Aqueous Two-Phase Systems for Bioprocess Development for the Recovery of Biological Products addresses specific applications of ATPS for the recovery and partial purification of high molecular weight compounds such as proteins, nucleic acids and polysaccharides, particulate bioproducts such as cells and organelles and low molecular weight compounds. Non-conventional strategies involving ATPS such as affinity systems, continuous liquid-liquid fractionation stages and the recovery from plant extracts are presented. Economic analysis of the application of ATPS in comparison to other fractionation techniques, particularly liquid chromatography, is considered, as are opportunity and current trends in the ATPS research area. Each chapter utilizes the contributors' experimental expertise in traditional and non-conventional ATPS strategies, as well as analysis of areas of opportunity and perspectives on the development and future applications of ATPS in both the lab and larger scale operations. The result is a thorough and singular overview of ATPS which has not been matched by any other text on the market.

Partitioning In Aqueous Two – Phase System

Author : Harry Walter
Publisher : Elsevier
ISBN 13 : 0323142915
Total Pages : 731 pages
Book Rating : 4.3/5 (231 download)

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Book Synopsis Partitioning In Aqueous Two – Phase System by : Harry Walter

Download or read book Partitioning In Aqueous Two – Phase System written by Harry Walter and published by Elsevier. This book was released on 2012-12-02 with total page 731 pages. Available in PDF, EPUB and Kindle. Book excerpt: Partitioning in Aqueous Two-Phase Systems: Theory, Methods, Uses, and Applications to Biotechnology is a collection of papers that discusses the applications of aqueous two-phase systems to problems of separation and extraction of macromolecules, organelles, and cells. Papers focus on the theoretical basis and the practical details of the procedures used. Some of the papers describe in one or a few steps how two components can be separated by the investigator manipulating their partitions so that one component is in one phase and the other component is in the other phase or at the interface. Investigators can also avail of developed batch extractions for plant organelles, cell membranes, nucleic acids, and proteins. The book cites as an example the partitioning of right-side-out and inside-out vesicles (obtained from fragments of thylakoid membranes) to the top and bottom phases, respectively, of a Dx-PEG system. Other papers describe the use of the countercurrent distribution when single extraction steps are not sufficient to produce a separation in materials that do not differ greatly in their partitioning behavior. The collection can prove valuable for bio-chemists, cellular biologists, micro-biologists, and developmental biologists.

The Science and Application of Aqueous Two-Phase Systems and Liquid-Liquid Phase Separation in Biotechnology and Bioengineering

Author : John Paul Frampton
Publisher : Frontiers Media SA
ISBN 13 : 288963356X
Total Pages : 117 pages
Book Rating : 4.8/5 (896 download)

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Book Synopsis The Science and Application of Aqueous Two-Phase Systems and Liquid-Liquid Phase Separation in Biotechnology and Bioengineering by : John Paul Frampton

Download or read book The Science and Application of Aqueous Two-Phase Systems and Liquid-Liquid Phase Separation in Biotechnology and Bioengineering written by John Paul Frampton and published by Frontiers Media SA. This book was released on 2020-01-13 with total page 117 pages. Available in PDF, EPUB and Kindle. Book excerpt: The phase separation of incompatible liquids has been a topic of significant importance in chemical and industrial engineering for many years. Well-understood examples of this phenomenon include the phase separation of oil with water and the phase separation of non-polar organic solvents with water. Similar behavior is observed when aqueous solutions of two or more incompatible polymers or polymers and salts are mixed. In these mixtures (referred to as aqueous two-phase systems), the separated phases are composed mostly of water. Aqueous two-phase systems have been used extensively for the extraction of high-value biological products from mixtures of biological materials. In recent years, aqueous two-phase systems have also found increased use as materials for streamlining and improving the capabilities of cell and molecular assays, and for the design of advanced cell culture systems. Similar behavior of biological materials in living systems has also been observed, with emerging roles in cell physiology.

Partitioning in Aqueous Two-phase Systems

Author : Harry Walter
Publisher : Orlando ; Toronto : Academic Press
ISBN 13 :
Total Pages : 744 pages
Book Rating : 4.3/5 (91 download)

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Book Synopsis Partitioning in Aqueous Two-phase Systems by : Harry Walter

Download or read book Partitioning in Aqueous Two-phase Systems written by Harry Walter and published by Orlando ; Toronto : Academic Press. This book was released on 1985 with total page 744 pages. Available in PDF, EPUB and Kindle. Book excerpt: Partitioning In Aqueous Two - Phase System ...

Principles of Multiple-Liquid Separation Systems

Author : Kit Wayne Chew
Publisher : Elsevier
ISBN 13 : 0323986463
Total Pages : 396 pages
Book Rating : 4.3/5 (239 download)

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Book Synopsis Principles of Multiple-Liquid Separation Systems by : Kit Wayne Chew

Download or read book Principles of Multiple-Liquid Separation Systems written by Kit Wayne Chew and published by Elsevier. This book was released on 2023-01-12 with total page 396 pages. Available in PDF, EPUB and Kindle. Book excerpt: Principles of Multiple-Liquid Separation Systems: Interaction, Application and Advancement describes the basic principles and advancements of multiple-liquid separation systems in downstream processing. Several important elements are included, such as the fundamental process and mechanisms of the multiple-liquid separation system, key principles of the interaction between different solvents and phase components, applications, and green solvents for the separation system. Furthermore, the book gives insights in commercializing this separation technique to industrial scale and making the process environmentally and economically sustainable. The book also presents constructive critics of this separation technique for both past and the latest findings. - Comprehensively reviews several advanced separation methods and their fundamentals in a single source - Covers a deep understanding of the interaction between various liquid phase techniques and the latest cases of advanced techniques applied in bioprocesses - Provides a critical and constructive judgement of costs and environmental sustainability of multiple-liquid separation systems

Separation and Purification Technologies in Biorefineries

Author : Shri Ramaswamy
Publisher : John Wiley & Sons
ISBN 13 : 111849346X
Total Pages : 730 pages
Book Rating : 4.1/5 (184 download)

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Book Synopsis Separation and Purification Technologies in Biorefineries by : Shri Ramaswamy

Download or read book Separation and Purification Technologies in Biorefineries written by Shri Ramaswamy and published by John Wiley & Sons. This book was released on 2013-02-04 with total page 730 pages. Available in PDF, EPUB and Kindle. Book excerpt: Separation and purification processes play a critical role in biorefineries and their optimal selection, design and operation to maximise product yields and improve overall process efficiency. Separations and purifications are necessary for upstream processes as well as in maximising and improving product recovery in downstream processes. These processes account for a significant fraction of the total capital and operating costs and also are highly energy intensive. Consequently, a better understanding of separation and purification processes, current and possible alternative and novel advanced methods is essential for achieving the overall techno-economic feasibility and commercial success of sustainable biorefineries. This book presents a comprehensive overview focused specifically on the present state, future challenges and opportunities for separation and purification methods and technologies in biorefineries. Topics covered include: Equilibrium Separations: Distillation, liquid-liquid extraction and supercritical fluid extraction. Affinity-Based Separations: Adsorption, ion exchange, and simulated moving bed technologies. Membrane Based Separations: Microfiltration, ultrafiltration and diafiltration, nanofiltration, membrane pervaporation, and membrane distillation. Solid-liquid Separations: Conventional filtration and solid-liquid extraction. Hybrid/Integrated Reaction-Separation Systems: Membrane bioreactors, extractive fermentation, reactive distillation and reactive absorption. For each of these processes, the fundamental principles and design aspects are presented, followed by a detailed discussion and specific examples of applications in biorefineries. Each chapter also considers the market needs, industrial challenges, future opportunities, and economic importance of the separation and purification methods. The book concludes with a series of detailed case studies including cellulosic bioethanol production, extraction of algae oil from microalgae, and production of biopolymers. Separation and Purification Technologies in Biorefineries is an essential resource for scientists and engineers, as well as researchers and academics working in the broader conventional and emerging bio-based products industry, including biomaterials, biochemicals, biofuels and bioenergy.

Separations Using Aqueous Phase Systems

Author : D. Fisher
Publisher : Springer Science & Business Media
ISBN 13 : 1468456679
Total Pages : 476 pages
Book Rating : 4.4/5 (684 download)

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Book Synopsis Separations Using Aqueous Phase Systems by : D. Fisher

Download or read book Separations Using Aqueous Phase Systems written by D. Fisher and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 476 pages. Available in PDF, EPUB and Kindle. Book excerpt: The use of aqueous two-phase systems for the partitioning of macromolecules, organelles and cells was originally developed by Per-Ake Albertsson in Sweden in the mid-fifties [1-3]. These systems were initially applied to separations of plant organelles and viruses but their use has now extended into most areas of cell biology and biochemistry [4,5]. Since 1979 biennial International Conferences on Partitioning in Aqueous Two-Phase Systems have been held in Los Angeles (1979), Sheffield (1981), Vancouver (1983) and Lund (1985). The 5th conference was held in Oxford from 23-28 August 1987 and was entitled "Advances in Separations Using Aqueous Phase Systems in Cell Biology and Biotechnology". It is the formal presentations from this meeting which comprise this volume. In contrast to earlier books on phase partitioning [4,5] this volume contains, for the first time, worldwide contributions from over sixty partitioners from a variety of scientific disciplines, thereby providing a detailed overview of the widespread application and potential of bioseparations using phase partitioning. Disciplines include Biophysics, Biochemistry, Cell Biology, Microbiology, Biotechnology and Process Engineering, in both academic and commercial establishments. These biennial conferences allow advances in these diverse partitioning fields to be reviewed and compared; they also provide an opportunity for those considering using phase partitioning to obtain information, advice and contacts. Attendance has grown steadily over the years and 140 scientists came to Oxford. The conference consisted of ten symposia on areas of application of partitioning which have been organised as specific chapters in this volume.

Bioseparation Engineering

Author : Ajay Kumar
Publisher : I. K. International Pvt Ltd
ISBN 13 : 9380026080
Total Pages : 223 pages
Book Rating : 4.3/5 (8 download)

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Book Synopsis Bioseparation Engineering by : Ajay Kumar

Download or read book Bioseparation Engineering written by Ajay Kumar and published by I. K. International Pvt Ltd. This book was released on 2009 with total page 223 pages. Available in PDF, EPUB and Kindle. Book excerpt: Bioseparation Engineering is meant for undergraduate and the postgraduate student community pursuing careers in Life Sciences. It concentrates on the more recent methods and techniques for separating components and products of the biotechnology industry. Each chapter deals with a specific type or area of application and includes information on the basic principles, industrial equipment available, commercial applications and an overview of current research and development. Main objective of the book is to provide in-depth knowledge of the subject in an interesting and paramount simple way

Highly Selective Separations in Biotechnology

Author : G. Street
Publisher : Springer Science & Business Media
ISBN 13 : 940111322X
Total Pages : 242 pages
Book Rating : 4.4/5 (11 download)

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Book Synopsis Highly Selective Separations in Biotechnology by : G. Street

Download or read book Highly Selective Separations in Biotechnology written by G. Street and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 242 pages. Available in PDF, EPUB and Kindle. Book excerpt: Success in meeting the challenge to produce the commercial products anticipated by the exploitation of biological processes depends upon provid ing effective separation protocols. Effectiveness can be measured in terms of selectivity, purity, resolution and validatory success. The major processing problems are associated with either the selective recovery of molecules which are present in low concentrations from complex mixtures or the selective removal of contaminants from the desired molecule. Central to the evolution of processes satisfying this demand are the regulatory requirements being imposed by governments on the purity of a product, especially in the health care market. Synthetic organic chemists are increasingly finding it advantageous to conduct one or more steps using either enzymic biotransformations where molecules with a single and consistent stereochemistry or chirality are required. The underlying princi ples behind the methods, techniques and processes currently being used and developed commercially rely upon the biospecific nature and properties of the desired molecule. When these factors are married to the more traditional techniques of precipitation, chromatography, liquid-liquid extraction and membrane processes, powerful tools emerge, allowing highly selective separations to be designed. The logical extension of these combinations is to apply genetic engineering techniques to influence the separations at a more fundamental and structural level by modifying the target protein at source, during its synthesis, to facilitate its separation in a given, selective manner, leading to the distinct possibility of producing 'designer' separation programmes.

Bioseparations of Proteins

Author : Ajit Sadana
Publisher : Elsevier
ISBN 13 : 0080528147
Total Pages : 367 pages
Book Rating : 4.0/5 (85 download)

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Book Synopsis Bioseparations of Proteins by : Ajit Sadana

Download or read book Bioseparations of Proteins written by Ajit Sadana and published by Elsevier. This book was released on 1997-11-27 with total page 367 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book covers the fundamentals of protein inactivation during bioseparation and the effect on protein processing. Bioseparation of Proteins is unique because it provides a background of the bioseparation processes, and it is the first book available to emphasize the influence of the different bioseparation processes on protein inactivation.Bioseparation of Proteins covers the extent, mechanisms of, and control of protein inactivation during these processes along with the subsequent and essential validation of these processes. The book focuses on the avoidance of protein (biologicalproduct) inactivation at each step in a bioprocess. It compares protein inactivation exhibited during the different bioseparation processes by different workers and provides a valuable framework for workers in different areas interested in bioseparations.Topics include separation and detection methods; estimates of protein inactivation and an analysis of this problem for different separation processes; strategies for avoiding inactivation; the molecular basis of surface activity and protein adsorption,process monitoring, and product validation techniques; and the economics of various bioseparation processes and quality control procedures.Key Features* Protein inactivation and other aspects of biological stability are critical to an effective bioseparation process; This book is a detailed and critical review of the available literature in an area that is essential to the effectiveness, validation, and economics of bioseparation processes for drugs and other biological products; Conveniently assembled under one cover, the survey of the literature and resulting perspective will greatly assist engineers and chemists in designingand improving their own processes; Key features of the text include:* detailed data on biological stability under various bioseparation conditions* extensive case studies from the literature on separation processes, validation, and economics* simplified analysis of protein refolding and inactivation mechanisms* consideration of adsorption theories and the effect of heterogeneity* coverage of both classical and novel bioseparation techniques, including chromatographic procedures

CRC Handbook of Thermodynamic Data of Polymer Solutions, Three Volume Set

Author : Christian Wohlfarth
Publisher : CRC Press
ISBN 13 : 1482274906
Total Pages : 1688 pages
Book Rating : 4.4/5 (822 download)

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Book Synopsis CRC Handbook of Thermodynamic Data of Polymer Solutions, Three Volume Set by : Christian Wohlfarth

Download or read book CRC Handbook of Thermodynamic Data of Polymer Solutions, Three Volume Set written by Christian Wohlfarth and published by CRC Press. This book was released on 2018-10-03 with total page 1688 pages. Available in PDF, EPUB and Kindle. Book excerpt: Providing valuable insight on physical behavior of polymer solutions, intermolecular interactions, and the molecular nature of mixtures, each volume in this one-of-a-kind handbook brings together reliable, easy-to-use entries, references, tables, examples, and appendices on experimental data from hundreds of primary journal articles, dissertations, and other published papers. This three-volume set presents hundreds of data sets including VLE/gas solubility isotherms, LLE and HPPE for polymer systems in supercritical fluids, as well as volumetric, enthalpic, and virial coefficient data sets, essential for handling industrial and laboratory processes involving all types of polymer systems.