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Download or read book Self-assembly of Recombinant Human Elastin Polypeptides with Potential for Use in Biomaterials Applications written by and published by . This book was released on 2001 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
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Author : Catherine M. (Catherine Mary) Bellingham
Publisher : National Library of Canada = Bibliothèque nationale du Canada
ISBN 13 : 9780612635920
Total Pages : 270 pages
Book Rating : 4.6/5 (359 download)
Download or read book Self-assembly of Recombinant Human Elastin Polypeptides with Potential for Use in Biomaterials Applications [microform] written by Catherine M. (Catherine Mary) Bellingham and published by National Library of Canada = Bibliothèque nationale du Canada. This book was released on 2001 with total page 270 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author : Catherine M. Bellingham
Publisher :
ISBN 13 :
Total Pages : 0 pages
Book Rating : 4.:/5 (133 download)
Download or read book Self-assembly of Recombinant Human Elastin Polypeptides with Potential for Use in Biomaterials Applications written by Catherine M. Bellingham and published by . This book was released on 2001 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Elastin is an extracellular matrix protein found in a number of tissues, including the large arteries such as the aorta, imparting the characteristics of extensibility and elastic recoil. Once laid down in tissues, polymeric elastin is not subject to turnover but is able to sustain its mechanical resilience through billions of cycles of extension and recoil. The process of ordered assembly of elastin into its extracellular, polymeric form remains one of the least well-understood steps in the biosynthesis of elastin. During this step, side chains of lysine residues in elastin monomers must be oxidatively deaminated and brought into juxtaposition in preparation for crosslinking. ' In vivo', several factors have been proposed to contribute to the alignment of elastin monomers in the formation of polymeric elastin, including a microfibrillar scaffold and a cell surface elastin binding protein. We have used a series of small, recombinant polypeptides based on sequences of human elastin to investigate the roles of various hydrophobic domains in promoting self-aggregation, and to determine whether this self-aggregation facilitates specific alignment of elastin polypeptides allowing crosslink formation at lysine residues. Our results demonstrate that polypeptides with as few as three hydrophobic and two crosslinking domains are able to self-aggregate into fibrillar structures essentially identical in appearance to those formed by the full-length elastin monomer, tropoelastin. Moreover, oxidation of lysine residues following aggregation, using a simple oxidizing agent (pyrroloquinoline quinone), results in spontaneous formation of lysine-derived covalent crosslinks between polypeptides, including desmosine and isodesmosine. Fabrication of these covalently crosslinked elastin polypeptides into membrane structures has also allowed assessment of their physical properties. Such membranes possess an elastic modulus, and extensibility and recoil properties similar to those of native insoluble elastin. These results strongly support the view that, independent of the influences of other factors, monomers of elastin possess an intrinsic ability to organize themselves into polymeric structures, aligning lysine residues for covalent crosslinking and forming matrices with elastomeric properties. Understanding the basis of the self-organizational ability of elastin-based polypeptides may provide important clues for the general design of self-assembling biomaterials.
Author : Helena S. Azevedo
Publisher : Woodhead Publishing
ISBN 13 : 0081020120
Total Pages : 614 pages
Book Rating : 4.0/5 (81 download)
Download or read book Self-assembling Biomaterials written by Helena S. Azevedo and published by Woodhead Publishing. This book was released on 2018-04-17 with total page 614 pages. Available in PDF, EPUB and Kindle. Book excerpt: Self-assembling biomaterials: molecular design, characterization and application in biology and medicine provides a comprehensive coverage on an emerging area of biomaterials science, spanning from conceptual designs to advanced characterization tools and applications of self-assembling biomaterials, and compiling the recent developments in the field. Molecular self-assembly, the autonomous organization of molecules, is ubiquitous in living organisms and intrinsic to biological structures and function. Not surprisingly, the exciting field of engineering artificial self-assembling biomaterials often finds inspiration in Biology. More important, materials that self-assemble speak the language of life and can be designed to seamlessly integrate with the biological environment, offering unique engineering opportunities in bionanotechnology. The book is divided in five parts, comprising design of molecular building blocks for self-assembly; exclusive features of self-assembling biomaterials; specific methods and techniques to predict, investigate and characterize self-assembly and formed assemblies; different approaches for controlling self-assembly across multiple length scales and the nano/micro/macroscopic properties of biomaterials; diverse range of applications in biomedicine, including drug delivery, theranostics, cell culture and tissue regeneration. Written by researchers working in self-assembling biomaterials, it addresses a specific need within the Biomaterials scientific community. Explores both theoretical and practical aspects of self-assembly in biomaterials Includes a dedicated section on characterization techniques, specific for self-assembling biomaterials Examines the use of dynamic self-assembling biomaterials
Author : Judith Tamara Cirulis
Publisher :
ISBN 13 : 9780494590607
Total Pages : 412 pages
Book Rating : 4.5/5 (96 download)
Download or read book Self-assembly and Fibre Formation of Elastin-like Polypeptides written by Judith Tamara Cirulis and published by . This book was released on 2009 with total page 412 pages. Available in PDF, EPUB and Kindle. Book excerpt: Elastin is a polymeric protein of the extracellular matrix that imparts the characteristics of extensibility and elastic recoil to tissues. Recombinant polypeptides based on the domain structures and sequences of human elastin self-assemble into organized fibrous structures, with physical properties similar to those of native polymeric elastin. Elastin self-assembly is initiated by a temperature-induced phase separation, called coacervation. Previous to this work, coacervation temperature had been the only parameter available to measure propensity for self-assembly. A variety of techniques were developed using spectrophotometry, microscopy, and rheometry to differentiate the stages of self-assembly, thereby enabling independent observation and quantitation of each stage, and allowing investigations into properties of polypeptides and solution conditions affecting these stages.Self-assembly in the presence of non-elastin, matrix-associated proteins showed that these proteins maintained the coacervate as small droplets, which sometimes flocculated into fibre-like structures. Rheometry demonstrated a second temperature-induced transition above the coacervation temperature, which resulted in gelation and viscoelastic characteristics similar to microgels.Kinetic analysis of self-assembly yielded two additional parameters: coacervation velocity and maturation velocity. Examining the effects of agitation, salt concentration, temperature, polypeptide concentration, size of a polypeptide, hydrophobic domain sequence, and cross-linking domain structure on the kinetics demonstrated that coacervation and maturation are independent stages of self-assembly involving distinct mechanisms. Microscopic observations showed that protein-rich droplets of coacervate grew by coalescence to a stable droplet size, which correlated to differences in maturation velocities between polypeptides. Coacervate droplet growth appeared limited by the formation of organized polypeptide at the surface of the droplets, decreasing surface fluidity. Many of the general principles of the physical chemistry of colloids and emulsions appeared to apply to the formation, growth and stabilization of coacervates of the elastin-like polypeptides.Together, these observations have resulted in a greater level of understanding of the entire self-assembly process, and provided a comprehensive model of elastin-like polypeptide self-assembly that relates to in vivo assembly of elastic fibres.
Author : Judith Cirulis
Publisher :
ISBN 13 :
Total Pages : pages
Book Rating : 4.:/5 (13 download)
Download or read book Self-assembly and Fibre Formation of Elastin-llke Polypeptides written by Judith Cirulis and published by . This book was released on with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author : Xuehai Yan
Publisher : John Wiley & Sons
ISBN 13 : 3527841253
Total Pages : 933 pages
Book Rating : 4.5/5 (278 download)
Download or read book Peptide Self-Assembly and Engineering written by Xuehai Yan and published by John Wiley & Sons. This book was released on 2024-02-09 with total page 933 pages. Available in PDF, EPUB and Kindle. Book excerpt: Peptide Self-Assembly and Engineering State-of-the-art research in peptide self-assembly, with coverage of fundamental aspects of how peptides self-assemble and an extensive number of applications Peptide Self-Assembly and Engineering: Fundamentals, Structures, and Applications (2V set) covers the latest progresses in the field of peptide self-assembly and engineering, including the fundamental principles of peptide self-assembly, new theory of nucleation and growth, thermodynamics and kinetics, materials design rules, and precisely controlled structures and unique functions. The broad contents from this book enable readers to obtain a systematical and comprehensive knowledge in the field of peptide self-assembly and engineering. Contributed by the leading scientists and edited by a highly qualified academic and an authority in the field, Peptide Self-Assembly and Engineering includes information on: Emerging areas in peptide assembly, such as immune agents, bioelectronics, energy conversion, flexible sensors, biomimetic catalysis, and more Existing applications in biomedical engineering, nanotechnology, and photoelectronics, including tissue engineering, drug delivery, and biosensing devices History of peptide self-assembly for design of functional materials and peptides’ unique mechanical, optical, electronic, and biological properties Various solvent conditions, such as pH, ionic strength, and polarity, that can affect the structure and stability of peptide assemblies A very comprehensive reference covering the latest progresses in the field of peptide self-assembly and engineering, Peptide Self-Assembly and Engineering is an essential resource for all scientists performing research intersecting with the subject, including biochemists, biotechnologists, pharmaceutical chemists, protein chemists, materials scientists, and medicinal chemists.
Author : Jaime Castillo
Publisher : CRC Press
ISBN 13 : 9814316946
Total Pages : 326 pages
Book Rating : 4.8/5 (143 download)
Download or read book Self-Assembled Peptide Nanostructures written by Jaime Castillo and published by CRC Press. This book was released on 2012-11-21 with total page 326 pages. Available in PDF, EPUB and Kindle. Book excerpt: The self-organization of bionanostructures into well-defined functional machineries found in nature has been a priceless source of ideas for researchers. The molecules of life, proteins, DNA, RNA, etc., as well as the structures and forms that these molecules assume serve as rich sources of ideas for scientists or engineers who are interested in developing bio-inspired materials for innovations in biomedical fields. In nature, molecular self-assembly is a process by which complex three-dimensional structures with well-defined functions are constructed, starting from simple building blocks such as proteins and peptides. This book introduces readers to the theory and mechanisms of peptide self-assembly processes. The authors present the more common peptide self-assembled building blocks and discuss how researchers from different fields can apply self-assembling principles to bionanotechnology applications. The advantages and challenges are mentioned together with examples that reflect the state of the art of the use of self-assembled peptide building blocks in nanotechnology.
Author : Bradley L. Nilsson
Publisher : Humana
ISBN 13 : 9781493978090
Total Pages : 0 pages
Book Rating : 4.9/5 (78 download)
Download or read book Peptide Self-Assembly written by Bradley L. Nilsson and published by Humana. This book was released on 2018-05-10 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume details methods and protocols on b-sheet assemblies and collagen. Divided into three parts chapters focus on expanding use of solid-state NMR as a powerful method to enhance structural understanding of self-assembled peptide materials, methods for the design, synthesis, and application of self-assembled peptide materials, and structural and mechanistic analyses of pathological amyloid systems that provide novel ways to assess function of the various possible aggregates as well to determine how the structure of these materials correlates to function/dysfunction in the biological context. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Peptide Self-Assembly: Methods and Protocols aims to capture modern methods that span the breadth of the exciting and expanding field of peptide self-assembly.
Author : Kevin Healy
Publisher : Elsevier
ISBN 13 : 0081006926
Total Pages : 4865 pages
Book Rating : 4.0/5 (81 download)
Download or read book Comprehensive Biomaterials II written by Kevin Healy and published by Elsevier. This book was released on 2017-05-18 with total page 4865 pages. Available in PDF, EPUB and Kindle. Book excerpt: Comprehensive Biomaterials II, Second Edition, Seven Volume Set brings together the myriad facets of biomaterials into one expertly-written series of edited volumes. Articles address the current status of nearly all biomaterials in the field, their strengths and weaknesses, their future prospects, appropriate analytical methods and testing, device applications and performance, emerging candidate materials as competitors and disruptive technologies, research and development, regulatory management, commercial aspects, and applications, including medical applications. Detailed coverage is given to both new and emerging areas and the latest research in more traditional areas of the field. Particular attention is given to those areas in which major recent developments have taken place. This new edition, with 75% new or updated articles, will provide biomedical scientists in industry, government, academia, and research organizations with an accurate perspective on the field in a manner that is both accessible and thorough. Reviews the current status of nearly all biomaterials in the field by analyzing their strengths and weaknesses, performance, and future prospects Covers all significant emerging technologies in areas such as 3D printing of tissues, organs and scaffolds, cell encapsulation; multimodal delivery, cancer/vaccine - biomaterial applications, neural interface understanding, materials used for in situ imaging, and infection prevention and treatment Effectively describes the many modern aspects of biomaterials from basic science, to clinical applications
Author : Stefan R. Schmidt
Publisher : John Wiley & Sons
ISBN 13 : 1118354583
Total Pages : 995 pages
Book Rating : 4.1/5 (183 download)
Download or read book Fusion Protein Technologies for Biopharmaceuticals written by Stefan R. Schmidt and published by John Wiley & Sons. This book was released on 2013-01-28 with total page 995 pages. Available in PDF, EPUB and Kindle. Book excerpt: The state of the art in biopharmaceutical FUSION PROTEIN DESIGN Fusion proteins belong to the most lucrative biotech drugs—with Enbrel® being one of the best-selling biologics worldwide. Enbrel® represents a milestone of modern therapies just as Humulin®, the first therapeutic recombinant protein for human use, approved by the FDA in 1982 and Orthoclone® the first monoclonal antibody reaching the market in 1986. These first generation molecules were soon followed by a plethora of recombinant copies of natural human proteins, and in 1998, the first de novo designed fusion protein was launched. Fusion Protein Technologies for Biopharmaceuticals examines the state of the art in developing fusion proteins for biopharmaceuticals, shedding light on the immense potential inherent in fusion protein design and functionality. A wide pantheon of international scientists and researchers deliver a comprehensive and complete overview of therapeutic fusion proteins, combining the success stories of marketed drugs with the dynamic preclinical and clinical research into novel drugs designed for as yet unmet medical needs. The book covers the major types of fusion proteins—receptor-traps, immunotoxins, Fc-fusions and peptibodies—while also detailing the approaches for developing, delivering, and improving the stability of fusion proteins. The main body of the book contains three large sections that address issues key to this specialty: strategies for extending the plasma half life, the design of toxic proteins, and utilizing fusion proteins for ultra specific targeting. The book concludes with novel concepts in this field, including examples of highly relevant multifunctional antibodies. Detailing the innovative science, commercial realities, and brilliant potential of fusion protein therapeutics, Fusion Protein Technologies for Biopharmaceuticals is a must for pharmaceutical scientists, biochemists, medicinal chemists, molecular biologists, pharmacologists, and genetic engineers interested in determining the shape of innovation in the world of biopharmaceuticals.
Author : Jill Lindsey Osborne
Publisher :
ISBN 13 : 9780494210383
Total Pages : 434 pages
Book Rating : 4.2/5 (13 download)
Download or read book Self-assembled Elastin-like Polypeptide Particles written by Jill Lindsey Osborne and published by . This book was released on 2006 with total page 434 pages. Available in PDF, EPUB and Kindle. Book excerpt: In this work, the self-assembly of a recombinant elastin-based block copolymer was investigated. The particle formation and dynamic behavior were characterized using inverted microscopy and dynamic light scattering. The morphology and stability were evaluated using scanning and transmission electron microscopy. Above a critical temperature the molecules self-assembled into a bimodal distribution of nano and micron-sized particles. The larger particles increased in size through coalescence. Micron-sized particle formation appeared largely reversible, although a self-assembly/disassembly hysteresis was observed. At high polyethylene glycol (PEG) concentrations particle coalescence and settling were reduced, particle stability seemed enhanced, and PEG coated the particles. Particle stabilization was also achieved through covalent crosslinking using glutaraldehyde. The particles captured a hydrophobic fluorescent probe with high efficiency and the data suggested a gradual release. This study laid the foundation for further particle size and stability optimization, and showed promise for particulate drug carriers using this family of elastin-based block copolymers.
Author : Peter R. Shewry
Publisher : Cambridge University Press
ISBN 13 : 1139438204
Total Pages : 409 pages
Book Rating : 4.1/5 (394 download)
Download or read book Elastomeric Proteins written by Peter R. Shewry and published by Cambridge University Press. This book was released on 2010-02-04 with total page 409 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book was originally published in 2002. Elastic proteins occur in a wide range of biological systems where they have evolved to fulfil precise biological roles. The best known include proteins in vertebrate muscles and connective tissues, such as titin, elastin and fibrillin, and spider silks. However, other examples include byssus and abductin from bivalve molluscs, resilin from arthropods and gluten from wheat. Interest in elastomeric proteins has been high for several reasons. Firstly, their biological and medical significance, particularly in human disease. Secondly, the unusual properties of proteins such as spider silks provide opportunities to develop materials. Thirdly, the development of scanning probe microscopy makes it possible to study structures and biomechanical properties of these proteins at the single molecule level. This book will be of value to anyone with an interest in the various aspects of elastomeric proteins.
Author : Ramanathan Nagarajan
Publisher : John Wiley & Sons
ISBN 13 : 1119001366
Total Pages : 364 pages
Book Rating : 4.1/5 (19 download)
Download or read book Self-Assembly written by Ramanathan Nagarajan and published by John Wiley & Sons. This book was released on 2019-01-07 with total page 364 pages. Available in PDF, EPUB and Kindle. Book excerpt: An introduction to the state-of-the-art of the diverse self-assembly systems Self-Assembly: From Surfactants to Nanoparticles provides an effective entry for new researchers into this exciting field while also giving the state of the art assessment of the diverse self-assembling systems for those already engaged in this research. Over the last twenty years, self-assembly has emerged as a distinct science/technology field, going well beyond the classical surfactant and block copolymer molecules, and encompassing much larger and complex molecular, biomolecular and nanoparticle systems. Within its ten chapters, each contributed by pioneers of the respective research topics, the book: Discusses the fundamental physical chemical principles that govern the formation and properties of self-assembled systems Describes important experimental techniques to characterize the properties of self-assembled systems, particularly the nature of molecular organization and structure at the nano, meso or micro scales. Provides the first exhaustive accounting of self-assembly derived from various kinds of biomolecules including peptides, DNA and proteins. Outlines methods of synthesis and functionalization of self-assembled nanoparticles and the further self-assembly of the nanoparticles into one, two or three dimensional materials. Explores numerous potential applications of self-assembled structures including nanomedicine applications of drug delivery, imaging, molecular diagnostics and theranostics, and design of materials to specification such as smart responsive materials and self-healing materials. Highlights the unifying as well as contrasting features of self-assembly, as we move from surfactant molecules to nanoparticles. Written for students and academic and industrial scientists and engineers, by pioneers of the research field, Self-Assembly: From Surfactants to Nanoparticles is a comprehensive resource on diverse self-assembly systems, that is simultaneously introductory as well as the state of the art.
Author : Morten Karsdal
Publisher : Academic Press
ISBN 13 : 0128098996
Total Pages : 274 pages
Book Rating : 4.1/5 (28 download)
Download or read book Biochemistry of Collagens, Laminins and Elastin written by Morten Karsdal and published by Academic Press. This book was released on 2016-07-29 with total page 274 pages. Available in PDF, EPUB and Kindle. Book excerpt: Biochemistry of Collagens, Laminins, and Elastin: Structure, Function, and Biomarkers provides a comprehensive introduction to collagen and structural proteins. Type I collagen is one of the most abundant molecules in the body, playing essential roles in different tissues, particularly bone and skin. A key aspect of type I collagen is its post-translational modifications which are essential for correct synthesis and structural integrity of collagens, for tissue-specific functionality, as well as for application as biomarkers of different pathologies. This volume summarizes current data on key structural proteins (collagens, laminins and elastin), reviews how these molecules affect pathologies, and describes selected modifications of proteins that result in altered signaling properties of the original extracellular matrix component. Further, it discusses the novel concept that an increasing number of components of the ECM harbor cryptic signaling functions that may be viewed as endocrine functions. Additionally, it highlights how this knowledge can be exploited to modulate fibrotic disease. Provides a comprehensive introduction to collagen and structural proteins Provides insight into emerging analytical technologies that can detect biomarkers of extracellular matrix degradation Includes a chapter dedicated to the biomarkers of structural proteins Contains insights into the biochemical interactions and changes to structural composition of proteins in disease states
Author :
Publisher :
ISBN 13 :
Total Pages : 972 pages
Book Rating : 4.F/5 ( download)
Download or read book Dissertation Abstracts International written by and published by . This book was released on 2002 with total page 972 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author : W.A. Linke
Publisher : Springer Science & Business Media
ISBN 13 : 9781402011917
Total Pages : 238 pages
Book Rating : 4.0/5 (119 download)
Download or read book Mechanics of Elastic Biomolecules written by W.A. Linke and published by Springer Science & Business Media. This book was released on 2003-05-31 with total page 238 pages. Available in PDF, EPUB and Kindle. Book excerpt: A representative cross-section of elastic biomolecules is covered in this volume, which combines seventeen contributions from leading research groups. State-of-the-art molecular mechanics experiments are described dealing with the elasticity of DNA and nucleoprotein complexes, titin and titin-like proteins in muscle, as well as proteins of the cytoskeleton and the extracellular matrix. The book speaks particularly to cell biologists, biophysicists, or bioengineers, and to senior researchers and graduate students alike, who are interested in recent advances in single-molecule technology (optical tweezers technique, atomic force microscopy), EM imaging, and computer simulation approaches to study nanobiomechanics. The findings discussed here have redefined our view of the role mechanical signals play in cellular functions and have greatly helped improve our understanding of biological elasticity in general.
