Science in a Technical World: The Plant Tissue Culture Industry

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Publisher : W. H. Freeman
ISBN 13 : 9780716737865
Total Pages : 100 pages
Book Rating : 4.7/5 (378 download)

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Book Synopsis Science in a Technical World: The Plant Tissue Culture Industry by : American Chemical Society (ACS)

Download or read book Science in a Technical World: The Plant Tissue Culture Industry written by American Chemical Society (ACS) and published by W. H. Freeman. This book was released on 2000-12-30 with total page 100 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Science in a Technical World

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Author :
Publisher : W H Freeman & Company
ISBN 13 : 9780716738084
Total Pages : pages
Book Rating : 4.7/5 (38 download)

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Book Synopsis Science in a Technical World by : American Chemical Society Acs

Download or read book Science in a Technical World written by American Chemical Society Acs and published by W H Freeman & Company. This book was released on 2000-02-28 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:

Science in a Technical World: The Plant Tissue Culture Industry CD-ROM

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Author :
Publisher : W. H. Freeman
ISBN 13 : 9780716737902
Total Pages : 60 pages
Book Rating : 4.7/5 (379 download)

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Book Synopsis Science in a Technical World: The Plant Tissue Culture Industry CD-ROM by : American Chemical Society (ACS)

Download or read book Science in a Technical World: The Plant Tissue Culture Industry CD-ROM written by American Chemical Society (ACS) and published by W. H. Freeman. This book was released on 2000-01-15 with total page 60 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Micropropagation

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Publisher : Springer Science & Business Media
ISBN 13 : 940092075X
Total Pages : 474 pages
Book Rating : 4.4/5 (9 download)

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Book Synopsis Micropropagation by : P. Debergh

Download or read book Micropropagation written by P. Debergh and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 474 pages. Available in PDF, EPUB and Kindle. Book excerpt: Micropropagation is a technology that has developed within the past 30 years. Earlier overviews of plant tissue culture have reviewed micropropagation as just one of many tissue culture procedures in use. Since the applications of this technology have multiplied so rapidly in recent years, we decided that a specific overview of the technology was now appropriate Our book begins with a review of the general principles of tissue culture as applied to micropropagation. This review is concise since the general topic has been covered in numerous other books and reviews. The basic principles of laboratory design and construction are summarized in the second chapter. Common problems encountered in micropropagation, both during and after culture, are examined in detail in four chapters. As micropropagation developed from a laboratory curiosity to a commercial industry, different considerations became important. These are discussed in two chapters. An attempt has been made to assess the current status of commercial production around the world. This has been difficult because commercial production figures are often closely guarded and little has been done to collect statistics on this growing industry. Applications to a broad range of crops are discussed in a series of chapters. These try to report the state of the art in each area, but since applications for some crops are much more advanced than for others, the focus of these chapters varies depending upon the progress that has been made.

Plant Tissue Culture Engineering

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Publisher : Springer Science & Business Media
ISBN 13 : 1402036949
Total Pages : 480 pages
Book Rating : 4.4/5 (2 download)

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Book Synopsis Plant Tissue Culture Engineering by : S. Dutta Gupta

Download or read book Plant Tissue Culture Engineering written by S. Dutta Gupta and published by Springer Science & Business Media. This book was released on 2006-07-10 with total page 480 pages. Available in PDF, EPUB and Kindle. Book excerpt: It is my privilege to contribute the foreword for this unique volume entitled: “Plant Tissue Culture Engineering,” edited by S. Dutta Gupta and Y. Ibaraki. While there have been a number of volumes published regarding the basic methods and applications of plant tissue and cell culture technologies, and even considerable attention provided to bioreactor design, relatively little attention has been afforded to the engineering principles that have emerged as critical contributions to the commercial applications of plant biotechnologies. This volume, “Plant Tissue Culture Engineering,” signals a turning point: the recognition that this specialized field of plant science must be integrated with engineering principles in order to develop efficient, cost effective, and large scale applications of these technologies. I am most impressed with the organization of this volume, and the extensive list of chapters contributed by expert authors from around the world who are leading the emergence of this interdisciplinary enterprise. The editors are to be commended for their skilful crafting of this important volume. The first two parts provide the basic information that is relevant to the field as a whole, the following two parts elaborate on these principles, and the last part elaborates on specific technologies or applications.

The Science of Plant Tissue Culture as a Catalyst for Agricultural and Industrial Development in an Emerging Economy

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Publisher :
ISBN 13 :
Total Pages : pages
Book Rating : 4.:/5 (115 download)

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Book Synopsis The Science of Plant Tissue Culture as a Catalyst for Agricultural and Industrial Development in an Emerging Economy by : S.E. Aladele

Download or read book The Science of Plant Tissue Culture as a Catalyst for Agricultural and Industrial Development in an Emerging Economy written by S.E. Aladele and published by . This book was released on 2012 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: The Science of Plant Tissue Culture as a Catalyst for Agricultural and Industrial Development in an Emerging Economy.

Plant Cell and Tissue Culture – A Tool in Biotechnology

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Publisher : Springer Nature
ISBN 13 : 303049098X
Total Pages : 459 pages
Book Rating : 4.0/5 (34 download)

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Book Synopsis Plant Cell and Tissue Culture – A Tool in Biotechnology by : Karl-Hermann Neumann

Download or read book Plant Cell and Tissue Culture – A Tool in Biotechnology written by Karl-Hermann Neumann and published by Springer Nature. This book was released on 2020-10-01 with total page 459 pages. Available in PDF, EPUB and Kindle. Book excerpt: This textbook is clearly structured with fourteen richly illustrated chapters and practical examples for easy understanding and direct implementation. The methods and findings developed in the authors’ group are presented in detailed, revised chapters. Readers will find valuable updates on the molecular basis of biotechnological processes, secondary metabolite production and genetic engineering. In addition, the basic principles of important biotechnologies, as well as examples of specially designed crops that deliver improved productivity under stress conditions, are presented. This second edition sets the direction for future research on the basic aspects of plant tissue culture and its applications in the fields of secondary metabolite production and genetic engineering. It provides both general and specific information for students, teachers, academic researchers and industrial teams who are interested in new developments in plant tissue culture and its applications.

Plant Cell and Tissue Culture for the Production of Food Ingredients

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Publisher : Springer Science & Business Media
ISBN 13 : 1461547539
Total Pages : 286 pages
Book Rating : 4.4/5 (615 download)

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Book Synopsis Plant Cell and Tissue Culture for the Production of Food Ingredients by : Tong-Jen Fu

Download or read book Plant Cell and Tissue Culture for the Production of Food Ingredients written by Tong-Jen Fu and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 286 pages. Available in PDF, EPUB and Kindle. Book excerpt: Commercial development of cultured-derived food ingredients has attracted interna tional interest. As consumers have become more health conscious in recent years, the de mand for natural food ingredients and disease-preventative phytochemicals has increased tremendously. Plant Cell and Tissue Culture provides an alternative method for controlled production of these products. A wide range of food ingredients has been shown to be pro duced in culture. Much progress has been made in advancing this technology to the point that large-scale production has become possible. This book is developed from the Symposium "Plant Cell and Tissue Culture for Food Ingredient Production" which was held on April 13-17, 1997 at the American Chemical So ciety National Meeting in San Francisco, CA. In this book, international experts in acade mia, government, and industry discuss current advances in the field of plant cell and tissue culture with special emphasis on its application for food ingredient production. Topics re lated to various aspects of plant cell and tissue culture technology are discussed, including overviews of recent advances in plant metabolic pathway studies, process development for improving yields, and bioreactor design and operation for large-scale production. Economic considerations and issues related to the commercial development of culture-derived food in gredients are discussed. Also included are the safety assessment schemes and regulatory frameworks set up by regulatory agencies around the world.

Commercial Scale Tissue Culture for Horticulture and Plantation Crops

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Publisher : Springer Nature
ISBN 13 : 9811900558
Total Pages : 338 pages
Book Rating : 4.8/5 (119 download)

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Book Synopsis Commercial Scale Tissue Culture for Horticulture and Plantation Crops by : Shubhpriya Gupta

Download or read book Commercial Scale Tissue Culture for Horticulture and Plantation Crops written by Shubhpriya Gupta and published by Springer Nature. This book was released on 2022-06-16 with total page 338 pages. Available in PDF, EPUB and Kindle. Book excerpt: This edited book is focusing on the novel and innovative procedures in tissue culture for large scale production of plantation and horticulture crops. It is bringing out a comprehensive collection of information on commercial scale tissue culture with the objective of producing high quality, disease-free and uniform planting material. Developing low cost commercial tissue culture can be one of the best possible way to attain the goal of sustainable agriculture. Tissue culture provides a means for rapid clonal propagation of desired cultivars, and a mechanism for somatic hybridization and in vitro selection of novel genotypes. Application of plant tissue culture technology in horticulture and plantation crops provides an efficient method to improve the quality and nutrition of the crops. This book includes a description of highly efficient, low cost in vitro regeneration protocols of important plantation and horticulture crops with a detailed guideline to establish a commercial plant tissue culture facility including certification, packaging and transportation of plantlets. The book discusses somatic embryogenesis, virus elimination, genetic transformation, protoplast fusion, haploid production, coculture of endophytic fungi, effects of light and ionizing radiation as well as the application of bioreactors. This book is useful for a wide range of readers such as, academicians, students, research scientists, horticulturists, agriculturists, industrial entrepreneurs, and agro-industry employees.

INTRODUCTION TO PLANT CELL TISSUE AND ORGAN CULTURE

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Publisher : PHI Learning Pvt. Ltd.
ISBN 13 : 8120346777
Total Pages : 334 pages
Book Rating : 4.1/5 (23 download)

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Book Synopsis INTRODUCTION TO PLANT CELL TISSUE AND ORGAN CULTURE by : SUNIL D. PUROHIT

Download or read book INTRODUCTION TO PLANT CELL TISSUE AND ORGAN CULTURE written by SUNIL D. PUROHIT and published by PHI Learning Pvt. Ltd.. This book was released on 2012-10-30 with total page 334 pages. Available in PDF, EPUB and Kindle. Book excerpt: Designed primarily as a text for undergraduate and postgraduate students of Botany and Plant Biotechnology, the book discusses the theoretical aspects and modern applications of plant cell, tissue and organ culture. Written with the aim of providing up-to-date information on the subject, and focused on the concept of commercialization of plant cell culture, the contents have been presented with clarity. The book not only discusses the theoretical aspects of plant tissue culture but also emphasizes the art of its practice. It also provides a systematic explanation of asepsis and methods of sterilization, plant tissue culture techniques, culture of reproductive structures, plant tissue culture in germplasm conservation, its applications in the industry and plant pathology and operation and management of greenhouse hardening unit. In addition, it discusses in vitro propagation of plants (micropropagation) with a series of case studies pertaining to tree species and horticultural crops. Besides students, the book will also prove to be useful for researchers, scholars and teachers.

Advances in Plant Tissue Culture

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Publisher : Academic Press
ISBN 13 : 0323998194
Total Pages : 428 pages
Book Rating : 4.3/5 (239 download)

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Book Synopsis Advances in Plant Tissue Culture by : Avinash Chandra Rai

Download or read book Advances in Plant Tissue Culture written by Avinash Chandra Rai and published by Academic Press. This book was released on 2022-05-28 with total page 428 pages. Available in PDF, EPUB and Kindle. Book excerpt: Advances in Plant Tissue Culture: Current Developments and Future Trends provides a complete and up-to-date text on all basic and applied aspects of plant tissue cultures and their latest application implications. It will be beneficial for students and early-career researchers of plant sciences and plant/agricultural biotechnology. Plant tissue culture has emerged as a sustainable way to meet the requirements of fresh produces, horticultural crops, medicinal or ornamental plants. Nowadays, plant tissue culture is an emerging filed applied in various aspects, including sustainable agriculture, plant breeding, horticulture and forestry. This book covers the latest technology, broadly applied for crop improvement, clonal propagation, Somatic hybridization Embryo rescue, Germplasm conservation, genetic conservation, or for the preservation of endangered species. However, these technologies also play a vital role in breaking seed dormancy over conventional methods of conservation. Focuses on plant tissue culture as an emerging field applied in various aspects, including sustainable agriculture, plant breeding, horticulture and forestry Includes current studies and innovations in biotechnology Covers commercialization and current perspectives in the field of plant tissue culture techniques

Plant Tissue Culture: An Introductory Text

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Publisher : Springer Science & Business Media
ISBN 13 : 8132210263
Total Pages : 318 pages
Book Rating : 4.1/5 (322 download)

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Book Synopsis Plant Tissue Culture: An Introductory Text by : Sant Saran Bhojwani

Download or read book Plant Tissue Culture: An Introductory Text written by Sant Saran Bhojwani and published by Springer Science & Business Media. This book was released on 2013-03-20 with total page 318 pages. Available in PDF, EPUB and Kindle. Book excerpt: Plant tissue culture (PTC) is basic to all plant biotechnologies and is an exciting area of basic and applied sciences with considerable scope for further research. PTC is also the best approach to demonstrate the totipotency of plant cells, and to exploit it for numerous practical applications. It offers technologies for crop improvement (Haploid and Triploid production, In Vitro Fertilization, Hybrid Embryo Rescue, Variant Selection), clonal propagation (Micropropagation), virus elimination (Shoot Tip Culture), germplasm conservation, production of industrial phytochemicals, and regeneration of plants from genetically manipulated cells by recombinant DNA technology (Genetic Engineering) or cell fusion (Somatic Hybridization and Cybridization). Considerable work is being done to understand the physiology and genetics of in vitro embryogenesis and organogenesis using model systems, especially Arabidopsis and carrot, which is likely to enhance the efficiency of in vitro regeneration protocols. All these aspects are covered extensively in the present book. Since the first book on Plant Tissue Culture by Prof. P.R. White in 1943, several volumes describing different aspects of PTC have been published. Most of these are compilation of invited articles by different experts or proceedings of conferences. More recently, a number of books describing the Methods and Protocols for one or more techniques of PTC have been published which should serve as useful laboratory manuals. The impetus for writing this book was to make available a complete and up-to-date text covering all basic and applied aspects of PTC for the students and early-career researchers of plant sciences and plant / agricultural biotechnology. The book comprises of nineteen chapters profusely illustrated with self-explanatory illustrations. Most of the chapters include well-tested protocols and relevant media compositions that should be helpful in conducting laboratory experiments. For those interested in further details, Suggested Further Reading is given at the end of each chapter, and a Subject and Plant Index is provided at the end of the book.

Experiments in Plant Tissue Culture

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Publisher : International Potato Center
ISBN 13 : 9780521315166
Total Pages : 260 pages
Book Rating : 4.3/5 (151 download)

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Book Synopsis Experiments in Plant Tissue Culture by : John H. Dodds

Download or read book Experiments in Plant Tissue Culture written by John H. Dodds and published by International Potato Center. This book was released on 1985-10-31 with total page 260 pages. Available in PDF, EPUB and Kindle. Book excerpt: The second edition of Experiments in Plant Tissue Culture makes available new information that has resulted from recent advances in the applications of plant tissue culture techniques to agriculture and industry. This comprehensive laboratory text takes the reader through a graded series of experimental protocols and also provides an introductory review of each topic. Topics include: a plant tissue culture laboratory, aseptic techniques, nutritional components of media, callus induction, organ formation, xylem cell differentiation, root cultures, cell suspensions, micropropagation, embryogenesis, isolation and fusion of protoplasts, haploid cultures, storage of plant genetic resources, secondary metabolite production, and quantification of procedures. This volume offers all of the basic experimental methods for the major research areas of plant tissue culture, and it will be invaluable to undergraduates and research investigators in the plant sciences.

Plant Tissue Culture

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Publisher : Academic Press
ISBN 13 : 0124159206
Total Pages : 206 pages
Book Rating : 4.1/5 (241 download)

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Book Synopsis Plant Tissue Culture by : Roberta H. Smith

Download or read book Plant Tissue Culture written by Roberta H. Smith and published by Academic Press. This book was released on 2013 with total page 206 pages. Available in PDF, EPUB and Kindle. Book excerpt: Plant Tissue Culture, Third Edition builds on the classroom tested, audience proven manual that has guided users through successful plant culturing A.tumefaciens mediated transformation, infusion technology, the latest information on media components and preparation, and regeneration and morphogenesis along with new exercises and diagrams provide current information and examples. The included experiments demonstrate major concepts and can be conducted with a variety of plant material that are readily available throughout the year. This book provides a diverse learning experience and is appropriate for both university students and plant scientists. Provides new exercises demonstrating tobacco leaf infiltration to observe transient expression of proteins and subcellular location of the protein, and information on development of a customized protocol for protoplast isolation for other experimental systems Includes detailed drawings that complement both introductions and experiments Guides reader from lab setup to supplies, stock solution and media preparation, explant selection and disinfestations, and experimental observations and measurement Provides the latest techniques and media information, including A. tumefaciens mediated transformation and infusion technology Fully updated literature

Plant Tissue Culture and Its Bio-technological Application

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Publisher : Springer
ISBN 13 : 9783540082279
Total Pages : 448 pages
Book Rating : 4.0/5 (822 download)

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Book Synopsis Plant Tissue Culture and Its Bio-technological Application by : W. Barz

Download or read book Plant Tissue Culture and Its Bio-technological Application written by W. Barz and published by Springer. This book was released on 1983-12-01 with total page 448 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Handbook on Plant and Cell Tissue Culture

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Publisher : ASIA PACIFIC BUSINESS PRESS Inc.
ISBN 13 : 8178330296
Total Pages : 605 pages
Book Rating : 4.1/5 (783 download)

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Book Synopsis Handbook on Plant and Cell Tissue Culture by : NIIR Board of Consultants and Engineers

Download or read book Handbook on Plant and Cell Tissue Culture written by NIIR Board of Consultants and Engineers and published by ASIA PACIFIC BUSINESS PRESS Inc.. This book was released on 2005-10-01 with total page 605 pages. Available in PDF, EPUB and Kindle. Book excerpt: Plants cell tissue culture is a rapidly developing technology which holds promise of restructuring agricultural and forestry practices. During the last two decades cell culture have made considerable advanced in the field of agriculture, horticulture, plant breeding, forestry, somatic cell genetics, phytopathology etc. Plant cells can be grown in isolation from intact plants in tissue culture systems. The cells have the characteristics of callus cells, rather than other plant cell types. These are the cells that appear on cut surfaces when a plant is wounded and which gradually cover and seal the damaged area. Plant cells and tissue culture are often used for the production of primary and secondary metabolites. Plant tissue cultures can be initiated from almost any part of a plant. The physiological state of the plant does have an influence on its response to attempts to initiate tissue culture. The parent plant must be healthy and free from obvious signs of disease or decay. The source, termed explant, may be dictated by the reason for carrying out the tissue culture. Younger tissue contains a higher proportion of actively dividing cells and is more responsive to a callus initiation programme. The plants themselves must be actively growing, and not about to enter a period of dormancy. Plant tissue culture is used widely in plant science; it also has a number of commercial applications. Tissue culture is employed in; micropropagation, elimination of pathogens from plant materials, germoplasm storage, production of somaclonal varients, embryo rescue, production of haploids, production of artificial seeds, production of secondary metabolities, production of transgenic plants etc. Some of the fundamentals of the book are plant tissue culture, basic requirements for tissue culture laboratory, surface sterilization of explant materials, development of tissue culture techniques, principles of cell culture cell, special factors influencing growth and metabolism, media for culturing cells and tissues, sterilisation procedures, design and equipment of a tissue culture laboratory, isolation method for microorganisms for culture, culture preservation and stability, genetic modification of industrial microorganisms mutation etc. The present book discuss about the methods, culture preservation and stability procedures, storage and transportation of plant cell tissue culture. This book is an invaluable resource for research workers, students, technocrats, entrepreneurs, institutional libraries etc. TAGS Plant Tissue Culture in India, Commercialization of Plant Tissue Culture in India, Role of Plant Tissue Culture in Agriculture, Plant Tissue Culture Industry in India, Industrial Plant Tissue Culture, Tissue Culture in Agriculture, Plant Tissue Culture, Tissue Culture, Cell Culture and Tissue Culture, Tissue Culture and Cell Culture, Tissue Culture in Plants, Plant Cell and Tissue Culture, Commercial Plant Tissue Culture in India, Plant Tissue Culture Business Plan, Plant Tissue Culture and Biotechnology, Tissue Culture Plants, Plant Tissue Culture Business Plan, Business Opportunities in Plant Tissue Culture, Tissue Culture Methods, Cybrid Production, Process of Cybrids Production, Production of Cybrids, Production of Cybrid Plants, Production of Haploid Plants, Haploid Production, Plant Secondary Metabolism, Production of Secondary Metabolites, Production of Secondary Metabolites Using Plant Cell Cultures, Plant Tissue Cultures in Production of Secondary Metabolites, Secondary Metabolites Production, Production of Somatic Hybrid Plants, Somatic Hybridization of Plants, Somatic Hybrid, Somatic Hybrid Production, Production of Enriched Biomass, Enrichment on Biomass Production, Formulation of Tissue Culture Medium, Collection of Explant Materials, Subculture of Callus, Regeneration of Plants from Callus, Preparation of Chick Embryo Extract, Preparation of Embryo Extract from Young Embryos, Preparation of Bovine Embryo Extract, Preparation of Eagles Medium, Media for Plant Tissues, Organ Culture, Preparation of Trypsinised Embryonic Carcass, Enrichment Culture Methods, Genetic Modification of Industrial Microorganisms Mutation, Methods Favouring Formation of Hybrid DNA Molecules, Modes of Growth of Bacteria and Fungi, Mixed Culture and Mixed Substrate Systems, Spontaneous Mixed Culture Process, Maintenance of Protoplasts, Collection of Plant Materials, Storage of Germ Plasm of Potato, Mammalian Embryonic Tissues, Preparation of Tissues from Plants, Largescale Culture Methods, Preparation and Sterilisation of Apparatus, Preparation and Sterilisation of Media, Reservation, Storage and Transportation of Living Tissues and Cells, Culture of Plant Cells for Extraction of Secondary Metabolites, Preparation of Explant, Suspension Culture, Extraction of Secondary Metabolites, Biotransformation in Plant Cells, Immobilization of Plant Cells, Special Tissue Culture Media, Manufacturing Plant Cultures, Products from Plant Tissue Culture, Cultivation of Plant Tissue, Cultures of Tomato Roots, Tissue Culture of Tomato Roots, Preparation of Carrot Callus Culture, Tissue Culture of Carrot Callus, Carrot Callus Tissue for Culture, Cultivation of Cells in Vivo Transplantation, Cultures on Agar, Npcs, Niir, Process Technology Books, Business Consultancy, Business Consultant, Project Identification and Selection, Preparation of Project Profiles, Startup, Business Guidance, Business Guidance to Clients, Startup Project, Startup Ideas, Project for Startups, Startup Project Plan, Business Start-Up, Business Plan for Startup Business, Great Opportunity for Startup, Small Start-Up Business Project, Best Small and Cottage Scale Industries, Startup India, Stand Up India, Small Scale Industries, New Small Scale Ideas for Haploid Production Industry, Cybrid Production Business Ideas You Can Start on Your Own, Indian Secondary Metabolites Production Industry, Small Scale Somatic Hybrid Production, Guide to Starting and Operating Small Business, Business Ideas for Enriched Biomass Production, How to Start Secondary Metabolites Production Business, Starting Enriched Biomass Production, Start Your Own Somatic Hybrid Production Business, Secondary Metabolites Production Business Plan, Business Plan for Cybrid Production, Small Scale Industries in India, Haploid Production Based Small Business Ideas in India, Small Scale Industry You Can Start on Your Own, Business Plan for Small Scale Industries, Set Up Cybrid Production, Profitable Small Scale Manufacturing, How to Start Small Business in India, Free Manufacturing Business Plans, Small and Medium Scale Manufacturing, Profitable Small Business Industries Ideas, Business Ideas for Startup

Plant Cell and Tissue Culture

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Author :
Publisher : Springer Science & Business Media
ISBN 13 : 364281784X
Total Pages : 85 pages
Book Rating : 4.6/5 (428 download)

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Book Synopsis Plant Cell and Tissue Culture by : J. Reinert

Download or read book Plant Cell and Tissue Culture written by J. Reinert and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 85 pages. Available in PDF, EPUB and Kindle. Book excerpt: The techniques of plant organ, tissue, and cell culture concentrated on reproducibility, simplicity and accu are now established in many research laboratories racy with sufficient illustration to make all mani throughout the world and are being used in numerous pulations clear. areas of plant science. Methods have been developed The drawings of items used in the bench layout to propagate plants and free them from viruses using diagrams are symbolic and are 'keyed in' by number to shoot tip culture. The regeneration of plants from callus the list of materials and equipment. A line around an culture has also proved useful commercially. Elegant item indicates that is sterile. techniques have been used to synthesise somatic The adoption of an integrated text in which diagrams hybrids by the fusion of protoplasts and to transform are related spatially to the methods will, we hope, help cells. These and many other techniques have been the student to grasp the techniques quickly and effec and can be used to investigate a variety of botanical tively. This is first and foremost a manual which has its phenomena as well as to improve crop plants and now place on the laboratory bench open in front of the provide an important part of the basic experimental student, a book to be used! skills required by a majority of experimental botanists.