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Regulation Of Attached Bacterial Growth By Adsorbed Proteins
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Book Synopsis Regulation of Attached Bacterial Growth by Adsorbed Proteins by :
Download or read book Regulation of Attached Bacterial Growth by Adsorbed Proteins written by and published by . This book was released on 1993 with total page 4 pages. Available in PDF, EPUB and Kindle. Book excerpt: The general objective of this project was to examine one component of the primary biofilm, protein, which has been hypothesized to be a major component of both dissolved and adsorbed organic pools in seawater. In previous work we found that surface energy was important in determining adsorption of protein to surfaces in seawater and degradation rates by bacteria and bacterial growth. In the last phase of this work, we examined the chemical nature of actual protein in seawater using HPLC techniques which we developed, standard chemical assays, and bioassays. We showed that protein in seawater is not the same chemically as cellular protein. Bacteria appear to treat nearly all seawater protein as if it were glycolated and degrade it at much lower rates than unmodified, fresh protein. Other experiments showed that cellular protein introduced into seawater is quickly modified by abiotic reactions and becomes less easily hydrolyzed by bacterial proteases within hours. The abiotic reactions appear to include adsorption to small colloids. These results begin to explain the growth dynamics of microorganisms on surfaces and the origin of refractory organic matter which is highly abundant in seawater ... Biofouling, Protein adsorption, Biofilm, Attached bacteria.
Book Synopsis Adsorption and Bacterial Adhesion Characteristics of Proteins, Microbial Growth Media and Milk on Abiotic Surfaces Under Static and Laminar Flow Conditions by : Nikhil Hiremath
Download or read book Adsorption and Bacterial Adhesion Characteristics of Proteins, Microbial Growth Media and Milk on Abiotic Surfaces Under Static and Laminar Flow Conditions written by Nikhil Hiremath and published by . This book was released on 2015 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: "Understanding the adhesion of bio-molecules and microorganisms to abiotic surfaces is an important consideration in food industry. The formation of these biofilms can have deleterious effects leading to contamination of food or materials coming into contact with them, causing spoilage &/or safety concerns. Among the biomolecules proteins are known to adhere to the surface very rapidly, followed by other biomolecules and microorganisms. In this study the two abiotic model surfaces, were conditioned with proteins [bovine serum albumin, sodium caseinate], microbial growth media [tryptic soya broth] and milk [whole milk, skim milk] under static and dynamic flow processing conditions. The extent of protein adsorbed to surfaces was quantified using the micro BCA protein assay. The food contact surfaces conditioned or without conditioning, were characterized using contact angle measurements to obtain various surfaces thermodynamic properties. The conditioning solutions were also characterized by dynamic light scattering and zeta potential measurements. The total free energy of interaction was calculated using the thermodynamic and the extended XDLVO models, and the adhesive potential of the biofilms was evaluated. The effect of preconditioning on the adhesion of bacteria to mica and polystyrene were studied. In addition the distribution of conditioning solutions and bacteria on the surface was evaluated using atomic force microscopy. The results under static conditions showed that the amount of proteins adsorbed on mica was much higher than polystyrene surfaces which may be because mica is more hydrophilic and polystyrene more hydrophobic. Sodium caseinate had the highest adsorption on both mica and polystyrene when compared to other conditioning solutions. The thermodynamic properties of conditioned surfaces showed that they favored electron-donating or Lewis base properties which are typical for proteins in hydrated states. DLS and Zeta potential results indicated that proteins were in aggregated states in solutions and had a negative surface charge. Preconditioning of mica and polystyrene surfaces altered their surface thermodynamic properties, especially, the electron donor and acceptor components of the surface free energy. This had a significant impact on the resulting hydrophobicity of the preconditioned surfaces as compared to the surfaces which were not conditioned. The computed free energy of interaction using the two models indicated the adhesion potential of conditioning solutions (in most cases) on both surfaces, but failed to accurately predict the extent of protein adsorption on surfaces. Under laminar flow conditions the amount of protein adsorbed onto mica and polystyrene surface was approximately two times higher than those obtained under static conditions. The DLS measurements of conditioning solutions showed that particle sizes of the conditioning solutions were more polydisperse than under static conditions. Dynamic flow condition also altered the surface thermodynamic properties of mica and polystyrene as observed with static conditions. The AFM analysis of both static and flow conditioned surfaces revealed that the conditioning film formed was not a continuous film but a heterogeneous deposit and attached in the form of aggregates. The adhesion of Listeria innocua to mica and polystyrene surfaces that were preconditioned with BSA, TSB and CAS under laminar flow showed lower adhesion values in comparison with those under static settings. However, with whole milk and skim milk, higher adhesion values of L. innocua were observed under flow conditions as compared with static conditions indicating bio-film source dependent variations. It was also observed that L. innocua cell adhesion on mica and polystyrene surfaces preconditioned with CAS WML and SML under static conditions were lower than Listeria monocytogenes under similar conditions, indicating strain dependent variations." --
Book Synopsis Scientific and Technical Aerospace Reports by :
Download or read book Scientific and Technical Aerospace Reports written by and published by . This book was released on 1994 with total page 892 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Microbial Biofilms by : Hilary M. Lappin-Scott
Download or read book Microbial Biofilms written by Hilary M. Lappin-Scott and published by Cambridge University Press. This book was released on 2003-09-18 with total page 332 pages. Available in PDF, EPUB and Kindle. Book excerpt: Biochemistry and ecology of biofilms from industrial, medical and other viewpoints.
Book Synopsis Molecular Biology of The Cell by : Bruce Alberts
Download or read book Molecular Biology of The Cell written by Bruce Alberts and published by . This book was released on 2002 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Microbial Adhesion and Aggregation by : K.C. Marshall
Download or read book Microbial Adhesion and Aggregation written by K.C. Marshall and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 423 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Peptides and Proteins as Biomaterials for Tissue Regeneration and Repair by : Mario Barbosa
Download or read book Peptides and Proteins as Biomaterials for Tissue Regeneration and Repair written by Mario Barbosa and published by Woodhead Publishing. This book was released on 2017-09-25 with total page 392 pages. Available in PDF, EPUB and Kindle. Book excerpt: Peptides and Proteins as Biomaterials for Tissue Regeneration and Repair highlights the various important considerations that go into biomaterial development, both in terms of fundamentals and applications. After covering a general introduction to protein and cell interactions with biomaterials, the book discusses proteins in biomaterials that mimic the extracellular matrix (ECM). The properties, fabrication and application of peptide biomaterials and protein-based biomaterials are discussed in addition to in vivo and in vitro studies. This book is a valuable resource for researchers, scientists and advanced students interested in biomaterials science, chemistry, molecular biology and nanotechnology. Presents an all-inclusive and authoritative coverage of the important role which protein and peptides play as biomaterials for tissue regeneration Explores protein and peptides from the fundamentals, to processing and applications Written by an international group of leading biomaterials researchers
Book Synopsis Physiological Models in Microbiology by : M. Bazin
Download or read book Physiological Models in Microbiology written by M. Bazin and published by CRC Press. This book was released on 2018-02-01 with total page 159 pages. Available in PDF, EPUB and Kindle. Book excerpt: Physiological Models in Microbiology consists of two volumes. Volume I considers models of basic growth processes and the effects of environmental factors on microbial growth. Volume II describes models of secondary processes, in particular, microbial death, spore germination, chemotaxis, and surface growth.
Book Synopsis Bacterial Adhesion to Cells and Tissues by : Itzhak Ofek
Download or read book Bacterial Adhesion to Cells and Tissues written by Itzhak Ofek and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 585 pages. Available in PDF, EPUB and Kindle. Book excerpt: Attachment to host cells or tissues is often the first step in the establishment of bacterial infections. A complex array of recognition, attachment, and virulence factors is involved in this process, which recent research has greatly illuminated. This comprehensive and authoritative volume discusses the specific cell and tissue-specific affinities of pathogenic microorganisms, including bioinorganic surfaces such as teeth, and is an essential reference for researchers and students of host-pathogen interactions.
Download or read book Bacteriophages written by David R. Harper and published by Springer Nature. This book was released on 2021-01-30 with total page 1376 pages. Available in PDF, EPUB and Kindle. Book excerpt: This first major reference work dedicated to the mannifold industrial and medical applications of bacteriophages provides both theoretical and practical insights into the emerging field of bacteriophage biotechnology. The book introduces to bacteriophage biology, ecology and history and reviews the latest technologies and tools in bacteriophage detection, strain optimization and nanotechnology. Usage of bacteriophages in food safety, agriculture, and different therapeutic areas is discussed in detail. This book serves as essential guide for researchers in applied microbiology, biotechnology and medicine coming from both academia and industry.
Download or read book Chemical Abstracts written by and published by . This book was released on 2002 with total page 2540 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Bio-Synthetic Hybrid Materials and Bionanoparticles by : Alexander Böker
Download or read book Bio-Synthetic Hybrid Materials and Bionanoparticles written by Alexander Böker and published by Royal Society of Chemistry. This book was released on 2015 with total page 328 pages. Available in PDF, EPUB and Kindle. Book excerpt: An overview of how biological structures can be used to produce new functional materials.
Book Synopsis Government Reports Annual Index by :
Download or read book Government Reports Annual Index written by and published by . This book was released on 1993 with total page 1298 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Industrial Biofouling by : T. Reg Bott
Download or read book Industrial Biofouling written by T. Reg Bott and published by Elsevier. This book was released on 2011-02-24 with total page 221 pages. Available in PDF, EPUB and Kindle. Book excerpt: Industrial Biofouling discusses the the challenges--and to a lesser extent, the benefits--of biofilms on industrial processing surfaces. It addresses the operating problems caused by establishment and growth of microorganisms, thereby enabling effective equipment design and operation that minimizes biofouling. Discusses the chemical and physical control of biofilm growth, with coverage of dosing techniques, equipment cleaning, and cost management Presents methods for monitoring and evaluating the effectiveness of control techniques Incorporates explicit figures and diagrams to aid in understanding
Book Synopsis Fermentation Microbiology and Biotechnology, Fourth Edition by : E. M. T. El-Mansi
Download or read book Fermentation Microbiology and Biotechnology, Fourth Edition written by E. M. T. El-Mansi and published by CRC Press. This book was released on 2018-12-17 with total page 662 pages. Available in PDF, EPUB and Kindle. Book excerpt: Fermentation Microbiology and Biotechnology, 4th Edition explores and illustrates the broad array of metabolic pathways employed for the production of primary and secondary metabolites, as well as biopharmaceuticals. This updated and expanded edition addresses the whole spectrum of fermentation biotechnology, from fermentation kinetics and dynamics to protein and co-factor engineering. It also sheds light on the new strategies employed by industrialist for increasing tolerance and endurance of microorganisms to the accumulation of toxic wastes in microbial-cell factories. The new edition builds upon the fine pedigree of its earlier predecessors and extends the spectrum of the book to reflect the multidisciplinary and buoyant nature of this subject area. Key Features Covers the whole spectrum of the field from fermentation kinetics to control of fermentation and protein engineering. Includes case studies specifically designed to illustrate industrial applications and current state-of-the-art technologies. Presents the contributions of eminent international academics and industrial experts. Offers new chapters addressing: The prospects and the role of bio-fuels refineries, Control of metabolic efflux to product formation in microbial-cell factories and Improving tolerance of microorganisms to toxic byproduct accumulation in the fermentation vessel.
Book Synopsis Biomaterials Science by : Buddy D. Ratner
Download or read book Biomaterials Science written by Buddy D. Ratner and published by Elsevier. This book was released on 2004-08-18 with total page 879 pages. Available in PDF, EPUB and Kindle. Book excerpt: The second edition of this bestselling title provides the most up-to-date comprehensive review of all aspects of biomaterials science by providing a balanced, insightful approach to learning biomaterials. This reference integrates a historical perspective of materials engineering principles with biological interactions of biomaterials. Also provided within are regulatory and ethical issues in addition to future directions of the field, and a state-of-the-art update of medical and biotechnological applications. All aspects of biomaterials science are thoroughly addressed, from tissue engineering to cochlear prostheses and drug delivery systems. Over 80 contributors from academia, government and industry detail the principles of cell biology, immunology, and pathology. Focus within pertains to the clinical uses of biomaterials as components in implants, devices, and artificial organs. This reference also touches upon their uses in biotechnology as well as the characterization of the physical, chemical, biochemical and surface properties of these materials. Provides comprehensive coverage of principles and applications of all classes of biomaterials Integrates concepts of biomaterials science and biological interactions with clinical science and societal issues including law, regulation, and ethics Discusses successes and failures of biomaterials applications in clinical medicine and the future directions of the field Cover the broad spectrum of biomaterial compositions including polymers, metals, ceramics, glasses, carbons, natural materials, and composites Endorsed by the Society for Biomaterials
Book Synopsis Microbial Growth in Drinking Water Supplies by : Dick van der Kooij
Download or read book Microbial Growth in Drinking Water Supplies written by Dick van der Kooij and published by IWA Publishing. This book was released on 2013-09-14 with total page 484 pages. Available in PDF, EPUB and Kindle. Book excerpt: Maintaining the microbial quality in distribution systems and connected installations remains a challenge for the water supply companies all over the world, despite many years of research. This book identifies the main concerns and knowledge gaps related to regrowth and stimulates cooperation in future research. Microbial Growth in Drinking Water Supplies provides an overview of the regrowth issue in different countries and the water quality problems related to regrowth. The book assesses the causes of regrowth in drinking water and the prevention of regrowth by water treatment and distribution. Editors: Dirk van der Kooij and Paul W.J.J. van der Wielen, KWR Watercycle Research Institute, The Netherlands
