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Author : Robert Pollack
Publisher : Cold Spring Harbor Laboratory Press
ISBN 13 :
Total Pages : 742 pages
Book Rating : 4.3/5 (91 download)
Download or read book Readings in Mammalian Cell Culture written by Robert Pollack and published by Cold Spring Harbor Laboratory Press. This book was released on 1981 with total page 742 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author : Mohamed Al-Rubeai
Publisher : Springer
ISBN 13 : 3319103202
Total Pages : 766 pages
Book Rating : 4.3/5 (191 download)
Download or read book Animal Cell Culture written by Mohamed Al-Rubeai and published by Springer. This book was released on 2014-11-28 with total page 766 pages. Available in PDF, EPUB and Kindle. Book excerpt: Animal cells are the preferred “cell factories” for the production of complex molecules and antibodies for use as prophylactics, therapeutics or diagnostics. Animal cells are required for the correct post-translational processing (including glycosylation) of biopharmaceutical protein products. They are used for the production of viral vectors for gene therapy. Major targets for this therapy include cancer, HIV, arthritis, cardiovascular and CNS diseases and cystic fibrosis. Animal cells are used as in vitro substrates in pharmacological and toxicological studies. This book is designed to serve as a comprehensive review of animal cell culture, covering the current status of both research and applications. For the student or R&D scientist or new researcher the protocols are central to the performance of cell culture work, yet a broad understanding is essential for translation of laboratory findings into the industrial production. Within the broad scope of the book, each topic is reviewed authoritatively by experts in the field to produce state-of-the-art collection of current research. A major reference volume on cell culture research and how it impacts on production of biopharmaceutical proteins worldwide, the book is essential reading for everyone working in cell culture and is a recommended volume for all biotechnology libraries.
Author : Jennie Mather
Publisher : Springer Science & Business Media
ISBN 13 : 1461593611
Total Pages : 378 pages
Book Rating : 4.4/5 (615 download)
Download or read book Mammalian Cell Culture written by Jennie Mather and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 378 pages. Available in PDF, EPUB and Kindle. Book excerpt: The advantages of obtaining a completely defined environment for the growth of cells in vitro were recognized very early in the history of cell culture (Lewis and Lewis, 1911). Continued interest in the nutritional requirements of cells in vitro and in providing an optimal environment for cells led to the development of the complex nutrient mixtures available today in many media (Waymouth, 1972; Ham, 1965). However, serum remained an essential component of medium for the growth of most cell types in culture. The question of what factor (or factors) in serum was essential for cell growth and survival remained unanswered for several decades. Initially, experiments were designed to purify the "active component" of serum for the growth of cells in culture. These experiments identified fetuin (Fisher et at., 1958) and nonsuppressible insulinlike activity (Temin et at., 1972) as important components of serum. However, the complexity of serum and the very low levels of active components in serum hindered progress in identi fying and isolating serum factors.
Author : Jennie Mather
Publisher :
ISBN 13 : 9781461593621
Total Pages : 304 pages
Book Rating : 4.5/5 (936 download)
Download or read book Mammalian Cell Culture written by Jennie Mather and published by . This book was released on 1984-06-01 with total page 304 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author : Ronald Acton
Publisher : Elsevier
ISBN 13 : 0323142613
Total Pages : 771 pages
Book Rating : 4.3/5 (231 download)
Download or read book Cell Culture and Its Application written by Ronald Acton and published by Elsevier. This book was released on 2012-12-02 with total page 771 pages. Available in PDF, EPUB and Kindle. Book excerpt: Cell Culture and Its Application covers the proceedings of the First International Cell Culture Congress Symposium, which focuses on how cell culture technology could impact on cell biology. The symposium aims to establish facilities for the cultivation of mammalian cells, which in turn would hopefully enhance basic cell biology research. The book is organized into four symposium and workshop sessions, encompassing 45 chapters. The opening chapter recognizes the interlocking relationship of cell culture technology and substantive cell biology. Chapters 2-5 describe the biochemical events that mark the cell cycle, with emphasis on occurrence of histone phosphorylation at each cycle. A discussion on cell differentiation, as a phenomena of interacting, inductive, and inhomogeneous cell populations, is included in these chapters. The second symposium session deals with signs of a revolution in progress in cell culture technology. This includes impact of tissue culture in physiological research course and in understanding of integrated physiology. The last two symposium sessions cover the large-scale production of virus from tissue cultures for cell antigens. An approach to the study of aging using diploid human cells in culture as a model system is also presented. It involves isolation and characterization of HLA antigens from cultured cells and their contribution to the study of disease. A brief discussion on mycoplasma contamination, microplasma-cell-virus interaction, and advantages and limitations of direct and indirect culture for primary isolation and detection of mycoplasma contamination is provided. The book then proceeds by discussing cell differentiation of specific cell or organ, such as testis, sensory cell, hepatocyte, embryonic muscle cell, and brain cortex. The concluding chapters cover nutritional requirements for cell growth, defined culture media for specific cell type, issues and problems related to large-scale cell production, and quality control. Cell biologists and researchers will find this book invaluable.
Author : Tapan Kumar Mukherjee
Publisher : Springer Nature
ISBN 13 : 9811917310
Total Pages : 1286 pages
Book Rating : 4.8/5 (119 download)
Download or read book Practical Approach to Mammalian Cell and Organ Culture written by Tapan Kumar Mukherjee and published by Springer Nature. This book was released on 2022-11-07 with total page 1286 pages. Available in PDF, EPUB and Kindle. Book excerpt: This Major Reference Work offers a detailed overview of culturing primary, secondary cell lines, tissues, and organs. It first introduces various types of mammalian cell cultures, infrastructure requirements for a mammalian cell-culture laboratory. The subsequent chapters present the detailed protocols for the isolation of mammalian hematologic organs and cells. It also discusses various cell-based assays for monitoring cell viability, cell proliferation, cytotoxicity, cell senescence, and cell death assays. In addition, the book addresses the various problems encountered while culturing animal cells, their possible causes, and suggested solutions, presenting detailed protocols for isolation and primary culturing of various mammalian cells and hematoimmunologic organs in two dimensions. Lastly, it reviews the various applications of animal-cell culture, stem-cell culture, and tissue and organ culture. As such, this reference book is highly relevant for students and professionals new to cell-culture work as well as to those wishing to expand their skills from cell-line cultures to primary cultures and from conventional 2D cultures to 3D cultures.
Author : Km Ramkumar
Publisher : CRC Press
ISBN 13 : 9781032494524
Total Pages : 0 pages
Book Rating : 4.4/5 (945 download)
Download or read book Advanced Mammalian Cell Culture Techniques written by Km Ramkumar and published by CRC Press. This book was released on 2023-10 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: This up-to-date book compiles the basic and advanced laboratory techniques of mammalian cell culture. It provides elaborate stepwise protocols, key points, and troubleshooting tips.
Author : Billie Ruth Bird
Publisher :
ISBN 13 :
Total Pages : 208 pages
Book Rating : 4.:/5 (31 download)
Download or read book Basic Laboratory Techniques in Cell Culture written by Billie Ruth Bird and published by . This book was released on 1981 with total page 208 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author :
Publisher :
ISBN 13 : 9784881710012
Total Pages : 207 pages
Book Rating : 4.7/5 (1 download)
Download or read book Mammalian Cell Culture Technology written by and published by . This book was released on 1985 with total page 207 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author : Lubiniecki
Publisher : Routledge
ISBN 13 : 1351435701
Total Pages : 650 pages
Book Rating : 4.3/5 (514 download)
Download or read book Large-Scale Mammalian Cell Culture Technology written by Lubiniecki and published by Routledge. This book was released on 2018-05-02 with total page 650 pages. Available in PDF, EPUB and Kindle. Book excerpt: An interdisciplinary approach, integrating biochemistry, biology, genetics, and engineering for the effective production of protein pharmaceuticals. The volume offers a biological perspective of large-scale animal cell culture and examines diverse processing strategies, process management, regulator
Author : Joseph Feder
Publisher :
ISBN 13 : 9780122504310
Total Pages : 159 pages
Book Rating : 4.5/5 (43 download)
Download or read book Large-scale Mammalian Cell Culture written by Joseph Feder and published by . This book was released on 1985 with total page 159 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author : Lubiniecki
Publisher : Routledge
ISBN 13 : 135143571X
Total Pages : 656 pages
Book Rating : 4.3/5 (514 download)
Download or read book Large-Scale Mammalian Cell Culture Technology written by Lubiniecki and published by Routledge. This book was released on 2018-05-02 with total page 656 pages. Available in PDF, EPUB and Kindle. Book excerpt: An interdisciplinary approach, integrating biochemistry, biology, genetics, and engineering for the effective production of protein pharmaceuticals. The volume offers a biological perspective of large-scale animal cell culture and examines diverse processing strategies, process management, regulator
Author : Alan Doyle
Publisher :
ISBN 13 :
Total Pages : 204 pages
Book Rating : 4.3/5 (91 download)
Download or read book Cell Culture written by Alan Doyle and published by . This book was released on 1997-07-04 with total page 204 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book provides concise practical instructions for setting up a cell culture facility and operating it effectively and safely. Procedures are described to guide the readers from the initiation of primary cultures, handling established cell lines and quantifying their performance, through to the production of specialist cell types by immortalization. The user-friendly format makes Mammalian Cell Culture: Essential Techniques a convenient summary of techniques for experienced workers as well as an invaluable aid to tissue culture in nonspecialized environments. The Essential Techniques Series books are designed to provide you with immediate access to the protocols you require every day. These handy pocket-sized manuals are easy to carry around, and conveniently spiral bound making them ideal for lab bench work. Written by experienced laboratory researchers, each book in the Essential Techniques Series gives up-to-date, tried and tested practical information for the life scientist. For each key technique these books: introduce the most commonly used methods, explain the advantages and disadvantages of the methods, and give advice on which procedure to use, provide easy to follow step-by-step protocols, with experimental notes and tips on where to pause, plus information on safety and suppliers.
Author : Leda Castilho
Publisher : Taylor & Francis
ISBN 13 : 1134099711
Total Pages : 752 pages
Book Rating : 4.1/5 (34 download)
Download or read book Animal Cell Technology written by Leda Castilho and published by Taylor & Francis. This book was released on 2008-03-01 with total page 752 pages. Available in PDF, EPUB and Kindle. Book excerpt: Animal Cell Technology: from Biopharmaceuticals to Gene Therapy provides a comprehensive insight into biological and engineering concepts related to mammalian and insect cell technology, as well as an overview of the applications of animal cell technology. Part 1 of the book covers the Fundamentals upon which this technology is based and covers the science underpinning the technology. Part 2 covers the Applications from the production of therapeutic proteins to gene therapy. The authors of the chapters are internationally-recognized in the field of animal cell culture research and have extensive experience in the areas covered in their respective chapters.
Author : Ralf Pörtner
Publisher : Humana
ISBN 13 : 9781071601907
Total Pages : 0 pages
Book Rating : 4.6/5 (19 download)
Download or read book Animal Cell Biotechnology written by Ralf Pörtner and published by Humana. This book was released on 2019-12-20 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Animal Cell Biotechnology: Methods and Protocols, Fourth Edition constitutes a comprehensive manual of state-of-the-art techniques for setting up mammalian cell lines and media for development of biopharmaceuticals, and optimizing critical parameters for cell culture considering the whole cascade from the lab to the final production. Special emphasis was put on model-assisted concepts. Scientists with long-refined expertise describe cutting-edge techniques for the production of therapeutic proteins and vaccines. Capturing the major advances that have occurred in both science and the technology of these biopharmaceuticals, this important book covers the powerful new techniques used in cell line and media development, optimizing process techniques and process strategies, use of model-assisted tools for process design and optimization, and in analysis. Topics include cell line and media development, techniques for process development, model-based techniques for process development, process analysis, and downstream techniques. The volume is divided into five parts that reflect the processes required for different stages of production. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Animal Cell Biotechnology: Methods and Protocols, Fourth Edition provides a compendium of techniques for scientists in industrial and research laboratories that use mammalian cells for biotechnology purposes.
Author : R. Ian Freshney
Publisher : John Wiley & Sons
ISBN 13 : 1118873645
Total Pages : 1615 pages
Book Rating : 4.1/5 (188 download)
Download or read book Culture of Animal Cells written by R. Ian Freshney and published by John Wiley & Sons. This book was released on 2015-12-23 with total page 1615 pages. Available in PDF, EPUB and Kindle. Book excerpt: Since the publication of the sixth edition of this benchmark text, numerous advances in the field have been made – particularly in stem cells, 3D culture, scale-up, STR profiling, and culture of specialized cells. Culture of Animal Cells: A Manual of Basic Technique and Specialized Applications, Seventh Edition is the updated version of this benchmark text, addressing these recent developments in the field as well as the basic skills and protocols. This eagerly awaited edition reviews the increasing diversity of the applications of cell culture and the proliferation of specialized techniques, and provides an introduction to new subtopics in mini-reviews. New features also include a new chapter on cell line authentication with a review of the major issues and appropriate protocols including DNA profiling and barcoding, as well as some new specialized protocols. Because of the continuing expansion of cell culture, and to keep the bulk of the book to a reasonable size, some specialized protocols are presented as supplementary material online. Culture of Animal Cells: A Manual of Basic Technique and Specialized Applications, Seventh Edition provides the most accessible and comprehensive introduction available to the culture and experimental manipulation of animal cells. This text is an indispensable resource for those in or entering the field, including academic research scientists, clinical and biopharmaceutical researchers, undergraduate and graduate students, cell and molecular biology and genetics lab managers, trainees and technicians.
Author : Thomas B. Shea
Publisher :
ISBN 13 : 9781524977283
Total Pages : pages
Book Rating : 4.9/5 (772 download)
Download or read book Techniques in Mammalian Cell Culture written by Thomas B. Shea and published by . This book was released on 2018-11-27 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
