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Proteomics Of Microbial Pathogens
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Book Synopsis Proteomics of Microbial Pathogens by : Peter R. Jungblut
Download or read book Proteomics of Microbial Pathogens written by Peter R. Jungblut and published by John Wiley & Sons. This book was released on 2007-02-27 with total page 343 pages. Available in PDF, EPUB and Kindle. Book excerpt: Containing proven, high-quality research articles selected from the popular Proteomics journal, this is a current overview of the latest research into the proteomics analysis of microbial pathogens as well as several review articles.
Book Synopsis Proteomics of Microbial Human Pathogens by : Nelson C. Soares
Download or read book Proteomics of Microbial Human Pathogens written by Nelson C. Soares and published by Frontiers Media SA. This book was released on 2017-01-17 with total page 154 pages. Available in PDF, EPUB and Kindle. Book excerpt: According to the World Health Organization (WHO), in 2012 infectious diseases and related conditions account for more than 70% of premature deaths across 22 African countries and estimated 450 000 people worldwide developed multi-drug resistant tuberculosis. This alarming situation, of great public health concern, calls for the urgent development of novel and efficient responding strategies. The employment of important research platforms, such as genomics and proteomics, has contributed significant insight into the mechanisms underlying microbial infection and microbe-host interaction. In this Frontiers Research Topic, we aim to produce a timely and pertinent discussion regarding the current status of “Proteomics of microbial Human pathogens” and the role of proteomics in combating the challenges posed by microbial infection and indeed acquired anti-microbial resistance. As the field of proteomics progressed from 2-DE gel based approaches to modern LC-MS/MS based workflows, remarkable advances have been reported in terms of data quantity and quality. Given the immediate and enormous advantages that high resolution and accurate mass spectrometers have brought to the field, proteomics has now evolved into a robust platform capable of generating large amounts of comprehensive data comparable to that reported previously in genomics studies. For example, detection of the complete yeast proteome has been reported and other small proteomes, such as those of bacteria, are within reach. Mass spectrometry-based proteomics has become an essential tool for biologists and biochemists, and is now considered by many as an essential component of modern structural biology. Additionally, the introduction of high-resolution mass spectrometers has driven the development of various different strategies aimed at accurate quantification of absolute and relative amount of protein(s) of interest. Emerging targeted mass spectrometry methodologies such as; Selected Reaction Monitoring (SRM), Parallel Reaction Monitoring (PRM) and SWATH, are perhaps the latest breakthrough within the proteomics community. Indeed, through a label free approach, targeted mass spectrometry offers an unequalled capability to characterize and quantify a specific set of proteins reproducibility, in any biological sample. Usefully, Aebersold and colleagues have recently generated and validated a number of assays to quantify 97% of the 4,012 annotated Mycobacterium tuberculosis (Mtb) proteins by SRM. As such, the Mtb Proteome library represents a valuable experimental resource that will undoubtedly bring new insight to the complex life cycle of Mtb. Finally, as reviewed recently in Frontiers Research Topic, mass spectrometry-based proteomics has had a tremendous impact on our current understanding of post translational modification (PTM) in bacteria including the key role of PTMs during interaction of pathogenic bacteria and host interactions. We believe that our understanding of microbial Human pathogens has benefited enormously from both 2-DE gel and modern LC-MS/MS based proteomics. It is our wish to produce an integrated discussion surrounding this topic to highlight the existing synergy between these research fields. We envisage this Research Topic as a window to expert opinions and perspectives on the realistic practicalities of proteomics as an important tool to address healthcare problems caused by microbial pathogens.
Book Synopsis Proteomics of Microorganisms by : Michael Hecker
Download or read book Proteomics of Microorganisms written by Michael Hecker and published by Springer Science & Business Media. This book was released on 2003-07-18 with total page 234 pages. Available in PDF, EPUB and Kindle. Book excerpt: Starting with the discovery of penicillin, other antibiotics, and insulin, the quest for understanding and use of biological systems, i. e. , microorganisms and ani mal tissue, for the production of value products has lead to a dramatic increase in microbiological and bioengineering research in the last decades. Chemical and pharmaceutical companies quickly realized the huge commercial potential of these bioproducts and have spent millions of US dollars on R &D as well as on a build up of production facilities. Although there was limited knowledge about the cell's molecular mechanisms, which are the basis for the formation of the desired products, products from fermentation and extraction of biological matrices were a success right from the start. R&D projects within industry and academia on the continuous improvement of production processes, especially microbial productivity and down stream processing, allowed a fast return of investment and secured competitiveness in the market. Whereas the focus of such research projects was mainly on the discovery of strains with higher pro ductivity for the product of interest, e. g. , antibiotics, a lot of expertise and knowledge was generated allowing the use of biotechnological products and processes outside the pharmaceutical arena. The tremendous increase in knowl edge and the technological developments in microbial genetics where driven by these research projects and, accompanied with the advancements in nucleotide chemistry leading to a much better understanding of intracellular processes, served as a basis for modern molecular biology and recombinant biotech nology.
Book Synopsis Proteomics of Microbial Human Pathogens by :
Download or read book Proteomics of Microbial Human Pathogens written by and published by . This book was released on 2017 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: According to the World Health Organization (WHO), in 2012 infectious diseases and related conditions account for more than 70% of premature deaths across 22 African countries and estimated 450 000 people worldwide developed multi-drug resistant tuberculosis. This alarming situation, of great public health concern, calls for the urgent development of novel and efficient responding strategies. The employment of important research platforms, such as genomics and proteomics, has contributed significant insight into the mechanisms underlying microbial infection and microbe-host interaction. In this Frontiers Research Topic, we aim to produce a timely and pertinent discussion regarding the current status of "Proteomics of microbial Human pathogens" and the role of proteomics in combating the challenges posed by microbial infection and indeed acquired anti-microbial resistance. As the field of proteomics progressed from 2-DE gel based approaches to modern LC-MS/MS based workflows, remarkable advances have been reported in terms of data quantity and quality. Given the immediate and enormous advantages that high resolution and accurate mass spectrometers have brought to the field, proteomics has now evolved into a robust platform capable of generating large amounts of comprehensive data comparable to that reported previously in genomics studies. For example, detection of the complete yeast proteome has been reported and other small proteomes, such as those of bacteria, are within reach. Mass spectrometry-based proteomics has become an essential tool for biologists and biochemists, and is now considered by many as an essential component of modern structural biology. Additionally, the introduction of high-resolution mass spectrometers has driven the development of various different strategies aimed at accurate quantification of absolute and relative amount of protein(s) of interest. Emerging targeted mass spectrometry methodologies such as; Selected Reaction Monitoring (SRM), Parallel Reaction Monitoring (PRM) and SWATH, are perhaps the latest breakthrough within the proteomics community. Indeed, through a label free approach, targeted mass spectrometry offers an unequalled capability to characterize and quantify a specific set of proteins reproducibility, in any biological sample. Usefully, Aebersold and colleagues have recently generated and validated a number of assays to quantify 97% of the 4,012 annotated Mycobacterium tuberculosis (Mtb) proteins by SRM. As such, the Mtb Proteome library represents a valuable experimental resource that will undoubtedly bring new insight to the complex life cycle of Mtb. Finally, as reviewed recently in Frontiers Research Topic, mass spectrometry-based proteomics has had a tremendous impact on our current understanding of post translational modification (PTM) in bacteria including the key role of PTMs during interaction of pathogenic bacteria and host interactions. We believe that our understanding of microbial Human pathogens has benefited enormously from both 2-DE gel and modern LC-MS/MS based proteomics. It is our wish to produce an integrated discussion surrounding this topic to highlight the existing synergy between these research fields. We envisage this Research Topic as a window to expert opinions and perspectives on the realistic practicalities of proteomics as an important tool to address healthcare problems caused by microbial pathogens.
Book Synopsis Mass Spectrometry for Microbial Proteomics by : Haroun N. Shah
Download or read book Mass Spectrometry for Microbial Proteomics written by Haroun N. Shah and published by John Wiley & Sons. This book was released on 2010-10-28 with total page 545 pages. Available in PDF, EPUB and Kindle. Book excerpt: New advances in proteomics, driven largely by developments in mass spectrometry, continue to reveal the complexity and diversity of pathogenic mechanisms among microbes that underpin infectious diseases. Therefore a new era in medical microbiology is demanding a rapid transition from current procedures to high throughput analytical systems for the diagnosis of microbial pathogens. This book covers the broad microbiological applications of proteomics and mass spectrometry. It is divided into six sections that follow the general progression in which most microbiology laboratories are approaching the subject –Transition, Tools, Preparation, Profiling by Patterns, Target Proteins, and Data Analysis.
Book Synopsis Proteomics for Studying Foodborne Microorganisms and Their Impact on Food Quality and Human Health by : Rosa Anna Siciliano
Download or read book Proteomics for Studying Foodborne Microorganisms and Their Impact on Food Quality and Human Health written by Rosa Anna Siciliano and published by Frontiers Media SA. This book was released on 2019-10-15 with total page 126 pages. Available in PDF, EPUB and Kindle. Book excerpt: Foodborne microorganisms play a pivotal role in mediating the tight relation between food and human health from a dual perspective. In fact, notwithstanding the introduction of strict regulations and new technologies to ensure food quality and safety, foodborne pathogens continue to cause infections and diseases and represent a serious public health concern, while spoilage bacteria can severely affect food quality thus leading to major industry and commercial losses. On the other hand, probiotics positively affect human health, promoting digestion and uptake of dietary nutrients, strengthening intestinal barrier function, modulating immune response and enhancing antagonism towards pathogens. Nowadays, proteomics represents a key discipline to perform high-throughput studies in the field of food microbiology and enables the most accurate identification of complex networks of proteins involved in the cell adaptation to different growth conditions as well as in beneficial or harmful effects on human health. The present eBook offers an overview of the most up to date proteomic methodologies and further assesses the strength of proteomics in exploring different aspects of the foodborne microorganism life-style and defining biomarkers of food quality and safety.
Book Synopsis Microbial Proteomics by : Dörte Becher
Download or read book Microbial Proteomics written by Dörte Becher and published by Humana. This book was released on 2019-10-11 with total page 344 pages. Available in PDF, EPUB and Kindle. Book excerpt: This detailed volume explores state-of-the-art methods for the identification, quantification, and characterization of microbial proteins. Split into five parts, the content addresses global sample preparation and protein enrichment, subcellular fractionation, protein quantification, analysis of post-translational protein modifications, as well as metaproteomics, a relatively new branch of microbial proteomics that investigates the proteins of all microbes comprising an environmental consortium. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Microbial Proteomics: Methods and Protocols serves as a valuable and stimulating source for all beginners and advanced researchers in the field of microbial proteomics and beyond. Chapter 18 is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.
Book Synopsis Microbial Proteomics by : Ian Humphery-Smith
Download or read book Microbial Proteomics written by Ian Humphery-Smith and published by John Wiley & Sons. This book was released on 2006-06-30 with total page 540 pages. Available in PDF, EPUB and Kindle. Book excerpt: Discover important lessons learned about whole organism biology via microbial proteomics This text provides an exhaustive analysis and presentation of current research in the field of microbial proteomics, with an emphasis on new developments and applications and future directions in research. The editors and authors show how and why the relative simplicity of microbes has made them attractive targets for extensive experimental manipulation in a quest for both improved disease prevention and treatment and an improved understanding of whole organism functional biology. In particular, the text demonstrates how microbial proteomic analyses can aid in drug discovery, including identification of new targets, novel diagnostic markers, and lead optimization. Each chapter is written by one or more leading experts in the field and carefully edited to ensure a consistent and thorough approach throughout. Methods, technologies, and tools associated with the most promising approaches are stressed. Key topics covered include: Microbial pathogenesis at the proteome level Whole cell modeling Structural proteomics and computational analysis Biomolecular interactions Physiological proteomics Metabolic reconstruction using proteomics data While presenting the practical utility of proteomics data, the text is also clear on the field's current limitations, pointing to areas where further investigation is needed. Offering a state-of-the-art perspective from internationally recognized experts, this text is ideally suited for researchers and students across the gamut of genomic sciences, including biochemistry, microbiology, molecular biology, genetics, biomedical and pharmaceutical sciences, biotechnology, and veterinary science.
Book Synopsis Genomics and Clinical Diagnostics by : David Whitehouse
Download or read book Genomics and Clinical Diagnostics written by David Whitehouse and published by Royal Society of Chemistry. This book was released on 2019-01-29 with total page 594 pages. Available in PDF, EPUB and Kindle. Book excerpt: Genomics and genome technology is having, and continues to have, a major impact on all areas of bioscience research providing insights into the key area of molecular mechanisms of cells in health and disease. This is causing a profound effect on biomedical science and is accelerating the development of new diagnostic applications. This book provides a timely, graduate level introduction to the fast-paced area of genomics and clinical diagnostic technologies and introduces the concept of applications based on this area. The initial chapters focus on principal molecular technologies that underpin the information in the later chapters. In addition to introductory areas of nucleic acids and techniques in molecular biology, bioinformatics and proteomics, other key diagnostic areas such as the use of immunological reagents are covered. The later chapters provide more specialised examples of currently used diagnostic technologies and insights into selected key diagnostic challenges including specific examples of molecular microbial diagnostics and molecular biomarkers in oncology. The running themes through the chapters provides an insight into current and future perspectives in this rapidly evolving field.
Book Synopsis Advances in microbial food safety by : P. Fratamico
Download or read book Advances in microbial food safety written by P. Fratamico and published by Elsevier Inc. Chapters. This book was released on 2013-07-31 with total page 46 pages. Available in PDF, EPUB and Kindle. Book excerpt: Omic technologies, including genomics, proteomics and metabolomics, are used to study pathogen behavior at the molecular level and develop improved pathogen detection and typing systems. Omic technologies analyze complete or nearly complete expressions of cell functions. DNA sequencing has resulted in complete genomes of foodborne pathogens. Omic-based technologies explore biological processes in a quantitative and integrative manner. They facilitate identification of genes and proteins that contribute to survival and persistence in food and other environments, that play a role in pathogenesis and that are targets for detection methods and control strategies. Challenges that remain are performing genomic and proteomic studies in food and other complex matrices and interpreting and analyzing the data produced from these investigations to enhance food safety.
Book Synopsis Microbial Proteomics: Development in Technologies and Applications by : Divakar Sharma
Download or read book Microbial Proteomics: Development in Technologies and Applications written by Divakar Sharma and published by Bentham Science Publishers. This book was released on 2020-12-31 with total page 236 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume brings current knowledge of proteomics technologies and related developments with special reference to diseases caused by microbes. The editor has compiled chapters written by expert academicians which distill the information about useful methods in microbial proteomics for the benefit of readers. Chapters cover several methods used to investigate the microbial proteome and special topics such as antimicrobial drug resistance mechanisms, biomarker developments, post translational modifications. Key Features: -overview of several biochemical methods in proteomics -full-color, high quality images of the most frequent technologies and applications -concise, well organized, and didactic format -updates in basic applied information -bibliographic references -information on proteomics for tuberculosis treatment This reference work is intended for researchers seeking information on laboratory techniques applied in proteomics research and microbiology.
Book Synopsis Proteomics in Foods by : Fidel Toldrá
Download or read book Proteomics in Foods written by Fidel Toldrá and published by Springer Science & Business Media. This book was released on 2012-12-16 with total page 589 pages. Available in PDF, EPUB and Kindle. Book excerpt: Food proteomics is one of the most dynamic and fast-developing areas in food science. The goal of this book is to be a reference guide on the principles and the current and future potential applications of proteomics in food science and technology. More specifically, the book will discuss recent developments and the expected trends of the near future in food proteomics. The book will be divided into two parts. The first part (7 chapters) will focus on the basic principles for proteomics, e.g., sample preparation, such as extraction and separation techniques, analytical instrumentation currently in use, and available databases for peptide and protein identification. The second part of the book (26 chapters) will focus on applications in foods. It will deal with quality issues related to post-mortem processes in animal foods and quality traits for all foods in general, as well as the identification of bioactive peptides and proteins, which are very important from the nutritional point of view. Furthermore, consumers are now extremely susceptible to food safety issues, and proteomics can provide reassurance with different safety aspects, such as food authenticity, detection of animal species in the food, and identification of food allergens. All of these issues will be covered in this book. It is also worth noting that both editors are internationally recognized experts in the field of food science, and both have edited numerous food science books and handbooks.
Book Synopsis Identifying Microbes by Mass Spectrometry Proteomics by : Charles H. Wick
Download or read book Identifying Microbes by Mass Spectrometry Proteomics written by Charles H. Wick and published by CRC Press. This book was released on 2013-09-10 with total page 282 pages. Available in PDF, EPUB and Kindle. Book excerpt: All microbes, including bacteria, viruses, and fungi, can be classified and identified by matching a few peptides known to be unique to each organism. Identifying Microbes by Mass Spectrometry Proteomics describes ways to identify microorganisms using powerful new techniques combining hardware and software and yielding highly accurate methods for detection, identification, and classification of microbes. This straightforward technology can be used to detect unknown and unsequenced microorganisms as well as microbes in complex environmental samples. This book reviews various mass analyzers used for detection and describes ionization methods frequently used for analysis of microbial constituents, a necessary step in the preparation of mass spectrometry (MS) samples. The text also discusses diverse processing methods, which are used to analyze MS files for matching mass spectral profiles, and examines protein and nucleic acid sequence-based methods capable of classification and identification of microbial agents. The book also covers sample collection methods and specific sample preparation techniques. The text addresses using computer software and bioinformatics approaches for data mining to discriminate microbes using mass spectrometry proteomics (MSP). It also discusses historical pattern recognition-based methods and other approaches such as analysis of pyrolysis products, chemical ionization (CI) of fatty acid methyl esters, and MALDI-MS. The text contains examples of the application of the MSP technique for microbe detection and includes a survey of suitable and commercially available MS-based platforms. Successful applications include the identification of unknown microbes in honey bees associated with colony collapse disorder and the analysis of virus strains from the 2009 influenza pandemic. The final chapter outlines future trends in these groundbreaking uses of MS techniques, which are fast, not limited by sample type, and show potential in answering complex environmental questions.
Book Synopsis Applied Genomics of Foodborne Pathogens by : Xiangyu Deng
Download or read book Applied Genomics of Foodborne Pathogens written by Xiangyu Deng and published by Springer. This book was released on 2017-01-23 with total page 213 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book provides a timely and thorough snapshot into the emerging and fast evolving area of applied genomics of foodborne pathogens. Driven by the drastic advance of whole genome shot gun sequencing (WGS) technologies, genomics applications are becoming increasingly valuable and even essential in studying, surveying and controlling foodborne microbial pathogens. The vast opportunities brought by this trend are often at odds with the lack of bioinformatics know-how among food safety and public health professionals, since such expertise is not part of a typical food microbiology curriculum and skill set. Further complicating the challenge is the large and ever evolving body of bioinformatics tools that can obfuscate newcomers to this area. Although reviews, tutorials and books are not in short supply in the fields of bioinformatics and genomics, until now there has not been a comprehensive and customized source of information designed for and accessible to microbiologists interested in applying cutting-edge genomics in food safety and public health research. This book fills this void with a well-selected collection of topics, case studies, and bioinformatics tools contributed by experts at the forefront of foodborne pathogen genomics research.
Book Synopsis Recent Developments in Applied Microbiology and Biochemistry by : Viswanath Buddolla
Download or read book Recent Developments in Applied Microbiology and Biochemistry written by Viswanath Buddolla and published by Academic Press. This book was released on 2020-10-15 with total page 382 pages. Available in PDF, EPUB and Kindle. Book excerpt: Recent Developments in Applied Microbiology and Biochemistry, Vol. 2, provides a comprehensive treatment and understanding on application oriented microbial concepts, giving readers insights into recent developments in microbial biotechnology and medical, agricultural and environmental microbiology. Discusses microbial proteome analyses and their importance in medical microbiology Explores emerging trends in the prevention of current global health problems, such as cancer, obesity and immunity Shows recent approaches in the production of novel enzymes from environmental samples by enrichment culture and metagenomics approaches Guides readers through the status and recent developments in analytical methods for the detection of foodborne microorganisms
Book Synopsis Physical and Functional Protein Interactomes of Pathogenic Gram-negative Bacteria by : Sarah L. Keasey
Download or read book Physical and Functional Protein Interactomes of Pathogenic Gram-negative Bacteria written by Sarah L. Keasey and published by . This book was released on 2017 with total page 438 pages. Available in PDF, EPUB and Kindle. Book excerpt: Protein interactomes of microbial pathogens comprise the total interactions of proteins that occur within the cell, and are roadmaps to the molecular processes and biochemical pathways that govern cellular functions and virulence. By examining isolated binary protein complexes in a cell-free environment, systems-level networks can be constructed from the bottom-up. Alternatively, by starting with the proteome, a systems-level network can be broken down from the top to identify interactions within whole cells. I explored these experimental methods with two Gram-negative pathogens, Yersinia pestis and Klebsiella pneumoniae. I used a proteome microarray of the plague bacterium Y. pestis to generate a database of>77,000 binary protein interactions. The database provided a means to understand Y. pestis proteins in the context of cognate interaction partners and a foundation to model the Y. pestis interactome from the bottom-up. I integrated sequence-based comparisons of homology, pathways analysis, protein abundance measurements, and transcript expression levels to reveal complexes and pathways involved in replication, biosynthesis, virulence, metabolism, and other diverse biological processes. To understand the proteomic response of bacteria that may circumvent antibiotic treatment with drugs that inhibit the ribosome, I developed a culture-based model of persistent infections with an invasive strain of extended-spectrum beta-lactamase producing K. pneumoniae to identify drug-specific networks from the top-down. The proteomes of cells that were tolerant to streptomycin or doxycycline, identified by label-free mass spectrometry, indicated fluctuations ({plusmn}2-fold) in the abundance of>600 proteins. I integrated pathways analysis, transcription factor binding sites, and experimental validation of predicted networks to reveal distinct bacterial responses that were exclusive of the anticipated ribosomal target and were unique to each drug. Because many hypothetical proteins were detected in the proteomic datasets of Y. pestis and K. pneumoniae, my results expand proteome annotation for these under-studied organisms. The approaches described here illustrate the application of proteome-scale interactomes for the prediction of networks that function within important human pathogens. Importantly, the interactomes of these Gram-negative pathogens are a potential source of targets for the development of new antimicrobials and vaccines, and offer considerations for understanding the emergence of multi-drug resistant bacteria.
Book Synopsis Activity-Based Protein Profiling by : Stephan A. Sieber
Download or read book Activity-Based Protein Profiling written by Stephan A. Sieber and published by Springer Science & Business Media. This book was released on 2012-02-21 with total page 175 pages. Available in PDF, EPUB and Kindle. Book excerpt: ABPP Methodology: Introduction and Overview, by Matthew B. Nodwell und Stephan A. Sieber Activity-Based Protein Profiling for Natural Product Target Discovery, by Joanna Krysiak und Rolf Breinbauer Photoaffinity Labeling in Activity-Based Protein Profiling, by Paul P. Geurink, Laurette M. Prely, Gijs A. van der Marel, Rainer Bischoff und Herman S. Overkleeft Application of Activity-Based Protein Profiling to the Study of Microbial Pathogenesis, by William P. Heal und Edward W. Tate Functional Analysis of Protein Targets by Metabolomic Approaches, by Yun-Gon Kim und Alan Saghatelian
