Read Books Online and Download eBooks, EPub, PDF, Mobi, Kindle, Text Full Free.
Proteomic Single Synapse Analysis With Array Tomography
Download Proteomic Single Synapse Analysis With Array Tomography full books in PDF, epub, and Kindle. Read online Proteomic Single Synapse Analysis With Array Tomography ebook anywhere anytime directly on your device. Fast Download speed and no annoying ads. We cannot guarantee that every ebooks is available!
Book Synopsis Proteomic Single-synapse Analysis with Array Tomography by : Brad Lee Busse
Download or read book Proteomic Single-synapse Analysis with Array Tomography written by Brad Lee Busse and published by Stanford University. This book was released on 2011 with total page 194 pages. Available in PDF, EPUB and Kindle. Book excerpt: Array tomography (AT) is a high-resolution proteomic imaging method that exploits a combination of light and EM techniques to resolve fine details at the synapse level across large fields of view spanning entire circuits. Much of my graduate work has centered on helping to shape AT into an imaging method fit for rigorous use, by developing computational algorithms which take advantage of AT's particular qualities to automate its operation. Among them are: a mapping tool for microscopy software to automate the imaging of AT's unique section layout; Multistackreg, an alignment plugin which is capable of registering multiple imaging sessions on the same piece of tissue; a cross-correlation algorithm for fluorescent channel validation; the synaptogram, a tool for visualizing synapse-scale high-dimensional proteomic molecular complexes; and an active learning paradigm for large-scale synapse quantification.
Book Synopsis Proteomic Single-synapse Analysis with Array Tomography by : Brad Lee Busse
Download or read book Proteomic Single-synapse Analysis with Array Tomography written by Brad Lee Busse and published by . This book was released on 2011 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: Array tomography (AT) is a high-resolution proteomic imaging method that exploits a combination of light and EM techniques to resolve fine details at the synapse level across large fields of view spanning entire circuits. Much of my graduate work has centered on helping to shape AT into an imaging method fit for rigorous use, by developing computational algorithms which take advantage of AT's particular qualities to automate its operation. Among them are: a mapping tool for microscopy software to automate the imaging of AT's unique section layout; Multistackreg, an alignment plugin which is capable of registering multiple imaging sessions on the same piece of tissue; a cross-correlation algorithm for fluorescent channel validation; the synaptogram, a tool for visualizing synapse-scale high-dimensional proteomic molecular complexes; and an active learning paradigm for large-scale synapse quantification.
Book Synopsis Proteomic Imaging of Synaptic Change by : Nicholas Collins Weiler
Download or read book Proteomic Imaging of Synaptic Change written by Nicholas Collins Weiler and published by . This book was released on 2013 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: Plasticity of connections within the neocortical microcircuit is thought to be the substrate for perceptual learning and memory. Mouse whisker barrel cortex offers many unique experimental advantages for the study of neocortical plasticity because of an accessible sensory periphery and a repeated, stereotyped columnar architecture. These features make it possible to characterize the microcircuitry of the cortical column, a potential common circuit element across species and cortical areas. I have developed an experimental and computational pipeline for characterizing the distribution of molecularly distinct synapse populations within whisker barrel columns using array tomography, a technique developed in our laboratory. I have performed preliminary explorations of modifications of the synaptic architecture of the mouse barrel cortex microcircuit produced by a brief period of sensory deprivation during early adulthood, and identify specific synapse populations most subject to such modification. I have also worked with collaborators at the Open Connectome Project to host this data and my software publicly online so that others can also mine it to explore the proteomic complexity of synaptic microcircuits.
Book Synopsis Cardiac Mapping by : Mohammad Shenasa
Download or read book Cardiac Mapping written by Mohammad Shenasa and published by John Wiley & Sons. This book was released on 2012-10-17 with total page 968 pages. Available in PDF, EPUB and Kindle. Book excerpt: Cardiac Mapping is the cardiac electrophysiologist’s GPS. It will guide you to new places in the heart and help you find the old places more easily...a valuable addition to your bookshelf Douglas P. Zipes, from the Foreword. Over the course of three previous editions, this book has become the acknowledged gold standard reference on the electro-anatomical mapping of the heart. This new edition features greatly expanded coverage—the number of chapters have doubled to 80 with 40 new chapters—on leading edge science, new clinical applications and future frontiers, authored by a who’s-who of global electrophysiology. This unique text offers truly comprehensive coverage of all areas of cardiac mapping, from core scientific principals to methodological and technical considerations to the latest data that you can put to work caring for patients. In addition, the all new 4th edition adds essential content on: Mapping in experimental models of arrhythmias Mapping supraventricular and ventricular tachyarrhythmias New catheter-based techniques Also featuring a companion website with video clips illustrating essential techniques described in the text The only state-of-the-art, stand-alone text on this dynamic subject, Cardiac Mapping is an essential resource for basic scientists, clinical electrophysiologists, cardiologists and all physicians who care for patients with cardiac arrhythmias.
Book Synopsis Intercellular Junctions—Advances in Research and Application: 2012 Edition by :
Download or read book Intercellular Junctions—Advances in Research and Application: 2012 Edition written by and published by ScholarlyEditions. This book was released on 2012-12-26 with total page 475 pages. Available in PDF, EPUB and Kindle. Book excerpt: Intercellular Junctions—Advances in Research and Application: 2012 Edition is a ScholarlyEditions™ eBook that delivers timely, authoritative, and comprehensive information about Intercellular Junctions. The editors have built Intercellular Junctions—Advances in Research and Application: 2012 Edition on the vast information databases of ScholarlyNews.™ You can expect the information about Intercellular Junctions in this eBook to be deeper than what you can access anywhere else, as well as consistently reliable, authoritative, informed, and relevant. The content of Intercellular Junctions—Advances in Research and Application: 2012 Edition has been produced by the world’s leading scientists, engineers, analysts, research institutions, and companies. All of the content is from peer-reviewed sources, and all of it is written, assembled, and edited by the editors at ScholarlyEditions™ and available exclusively from us. You now have a source you can cite with authority, confidence, and credibility. More information is available at http://www.ScholarlyEditions.com/.
Download or read book Cellular Imaging written by Eric Hanssen and published by Springer. This book was released on 2017-12-30 with total page 339 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book highlights important techniques for cellular imaging and covers the basics and applications of electron tomography and related techniques. In addition, it considers practical aspects and broadens the technological focus by incorporating techniques that are only now becoming accessible (e.g. block face imaging). The first part of the book describes the electron microscopy 3D technique available to scientists around the world, allowing them to characterize organelles, cells and tissues. The major emphasis is on new technologies like scanning transmission electron microscopy (STEM) tomography, though the book also reviews some of the more proven technologies like electron tomography. In turn, the second part is dedicated to the reconstruction of data sets, signal improvement and interpretation
Book Synopsis Biological Field Emission Scanning Electron Microscopy, 2 Volume Set by : Roland A. Fleck
Download or read book Biological Field Emission Scanning Electron Microscopy, 2 Volume Set written by Roland A. Fleck and published by John Wiley & Sons. This book was released on 2019-04-29 with total page 741 pages. Available in PDF, EPUB and Kindle. Book excerpt: The go‐to resource for microscopists on biological applications of field emission gun scanning electron microscopy (FEGSEM) The evolution of scanning electron microscopy technologies and capability over the past few years has revolutionized the biological imaging capabilities of the microscope—giving it the capability to examine surface structures of cellular membranes to reveal the organization of individual proteins across a membrane bilayer and the arrangement of cell cytoskeleton at a nm scale. Most notable are their improvements for field emission scanning electron microscopy (FEGSEM), which when combined with cryo-preparation techniques, has provided insight into a wide range of biological questions including the functionality of bacteria and viruses. This full-colour, must-have book for microscopists traces the development of the biological field emission scanning electron microscopy (FEGSEM) and highlights its current value in biological research as well as its future worth. Biological Field Emission Scanning Electron Microscopy highlights the present capability of the technique and informs the wider biological science community of its application in basic biological research. Starting with the theory and history of FEGSEM, the book offers chapters covering: operation (strengths and weakness, sample selection, handling, limitations, and preparation); Commercial developments and principals from the major FEGSEM manufacturers (Thermo Scientific, JEOL, HITACHI, ZEISS, Tescan); technical developments essential to bioFEGSEM; cryobio FEGSEM; cryo-FIB; FEGSEM digital-tomography; array tomography; public health research; mammalian cells and tissues; digital challenges (image collection, storage, and automated data analysis); and more. Examines the creation of the biological field emission gun scanning electron microscopy (FEGSEM) and discusses its benefits to the biological research community and future value Provides insight into the design and development philosophy behind current instrument manufacturers Covers sample handling, applications, and key supporting techniques Focuses on the biological applications of field emission gun scanning electron microscopy (FEGSEM), covering both plant and animal research Presented in full colour An important part of the Wiley-Royal Microscopical Series, Biological Field Emission Scanning Electron Microscopy is an ideal general resource for experienced academic and industrial users of electron microscopy—specifically, those with a need to understand the application, limitations, and strengths of FEGSEM.
Book Synopsis Neural Circuits Revealed by : Mariano Soiza-Reilly
Download or read book Neural Circuits Revealed written by Mariano Soiza-Reilly and published by Frontiers Media SA. This book was released on 2015-09-14 with total page 183 pages. Available in PDF, EPUB and Kindle. Book excerpt: Deciphering anatomical and functional maps in the nervous system is a main challenge for both clinical and basic neuroscience. Modern approaches to mark and manipulate neurons are bringing us closer than ever to better understand nervous system wiring diagrams. Here we present both original research and review material on current work in this area. Together, this eBook aims to provide a comprehensive snapshot of some of the tools and technologies currently available to investigate brain wiring and function, as well as discuss ongoing challenges the field will be confronted with in the future.
Book Synopsis Synapse Development and Maturation by : Pasko Rakic
Download or read book Synapse Development and Maturation written by Pasko Rakic and published by Academic Press. This book was released on 2020-05-29 with total page 560 pages. Available in PDF, EPUB and Kindle. Book excerpt: Synapse Development and Maturation, the latest release in the Comprehensive Developmental Neuroscience series, presents the latest information on the genetic, molecular and cellular mechanisms of neural development. The book provides a much-needed update that underscores the latest research in this rapidly evolving field, with new section editors discussing the technological advances that are enabling the pursuit of new research on brain development. This volume focuses on the synaptogenesis and developmental sequences in the maturation of intrinsic and synapse-driven patterns. Features leading experts in various subfields as section editors and article authors Presents articles that have been peer reviewed to ensure accuracy, thoroughness and scholarship Includes coverage of mechanisms which regulate synapse formation and maintenance during development Covers neural activity, from cell-intrinsic maturation, to early correlated patterns of activity
Book Synopsis Comprehensive Developmental Neuroscience: Cellular Migration and Formation of Neuronal Connections by : S.J. Le Marchand
Download or read book Comprehensive Developmental Neuroscience: Cellular Migration and Formation of Neuronal Connections written by S.J. Le Marchand and published by Elsevier Inc. Chapters. This book was released on 2013-05-06 with total page 63 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis New Techniques in Systems Neuroscience by : Adam D. Douglass
Download or read book New Techniques in Systems Neuroscience written by Adam D. Douglass and published by Springer. This book was released on 2015-04-13 with total page 309 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume is essential reading for anyone wishing to understand the recent explosion of experimental tools in neuroscience that now make it possible to manipulate, record, and understand neuronal activity within the intact brain, and which are helping us learn how the many neurons that comprise a network act together to control behavior. Leaders in the field discuss the latest developments in optogenetics, functional imaging, circuit mapping, and the application of these tools to complex biological problems.
Book Synopsis Building up the inhibitory synapse by : Enrico Cherubini
Download or read book Building up the inhibitory synapse written by Enrico Cherubini and published by Frontiers E-books. This book was released on with total page 159 pages. Available in PDF, EPUB and Kindle. Book excerpt: Fast inhibitory transmission exerts a powerful control on neuronal excitability and network oscillations thought to be associated with high cognitive functions. An alteration of inhibitory signaling is associated with major neurological and psychiatric disorders including epilepsy. Once released from presynaptic nerve terminals, GABA and glycine cross the synaptic cleft and bind to postsynaptic receptors localized in precise apposition to presynaptic release sites. The functional organization of inhibitory synapses consists in a dynamic process which relies on a number of highly specialized proteins that ensure the correct targeting, clustering, stabilization and subsequent fate of synaptic receptors. Among the proteins involved in this task, the tubulin-binding protein gephyrin plays a crucial role. Through its self-oligomerization properties, this protein forms hexagonal lattices that trap GABAA and glycine receptors and link them to the cytoskeleton. By directly interacting with cell-adhesion molecules of the neuroligin-neurexin families that connect presynaptic and postsynaptic neurons at synapses, gephyrin ensures a backward control of presynaptic signaling. In addition, changes in clusters size is dynamically regulated by lateral diffusion of neurotransmitter receptors between the synaptic and extrasynaptic compartments and by their interaction with synaptic scaffold proteins. The aim of this Research Topic (research articles and reviews) is to bring together experts on the cellular and molecular mechanisms regulating the appropriate assembly, location and function of pre and postsynaptic specializations at inhibitory synapses. A particular emphasis will be on the role of receptor trafficking in synaptic stabilization and plasticity.
Book Synopsis Correlative Imaging by : Paul Verkade
Download or read book Correlative Imaging written by Paul Verkade and published by John Wiley & Sons. This book was released on 2019-09-04 with total page 248 pages. Available in PDF, EPUB and Kindle. Book excerpt: Brings a fresh point of view to the current state of correlative imaging and the future of the field This book provides contributions from international experts on correlative imaging, describing their vision of future developments in the field based on where it is today. Starting with a brief historical overview of how the field evolved, it presents the latest developments in microscopy that facilitate the correlative workflow. It also discusses the need for an ideal correlative probe, applications in proteomic and elemental analysis, interpretation methods, and how correlative imaging can incorporate force microscopy, soft x-ray tomography, and volume electron microscopy techniques. Work on placing individual molecules within cells is also featured. Correlative Imaging: Focusing on the Future offers in-depth chapters on: correlative imaging from an LM perspective; the importance of sample processing for correlative imaging; correlative light and volume EM; correlation with scanning probe microscopies; and integrated microscopy. It looks at: cryo-correlative microscopy; correlative cryo soft X-ray imaging; and array tomography. Hydrated-state correlative imaging in vacuo, correlating data from different imaging modalities, and big data in correlative imaging are also considered. Brings a fresh view to one of the hottest topics within the imaging community: the correlative imaging field Discusses current research and offers expert thoughts on the field’s future developments Presented by internationally-recognized editors and contributors with extensive experience in research and applications Of interest to scientists working in the fields of imaging, structural biology, cell biology, developmental biology, neurobiology, cancer biology, infection and immunity, biomaterials and biomedicine Part of the Wiley–Royal Microscopical Society series Correlative Imaging: Focusing on the Future will appeal to those working in the expanding field of the biosciences, correlative microscopy and related microscopic areas. It will also benefit graduate students working in microscopy, as well as anyone working in the microscopy imaging field in biomedical research.
Book Synopsis Computational and Analytical Mathematics by : David H. Bailey
Download or read book Computational and Analytical Mathematics written by David H. Bailey and published by Springer Science & Business Media. This book was released on 2013-09-15 with total page 710 pages. Available in PDF, EPUB and Kindle. Book excerpt: The research of Jonathan Borwein has had a profound impact on optimization, functional analysis, operations research, mathematical programming, number theory, and experimental mathematics. Having authored more than a dozen books and more than 300 publications, Jonathan Borwein is one of the most productive Canadian mathematicians ever. His research spans pure, applied, and computational mathematics as well as high performance computing, and continues to have an enormous impact: MathSciNet lists more than 2500 citations by more than 1250 authors, and Borwein is one of the 250 most cited mathematicians of the period 1980-1999. He has served the Canadian Mathematics Community through his presidency (2000–02) as well as his 15 years of editing the CMS book series. Jonathan Borwein’s vision and initiative have been crucial in initiating and developing several institutions that provide support for researchers with a wide range of scientific interests. A few notable examples include the Centre for Experimental and Constructive Mathematics and the IRMACS Centre at Simon Fraser University, the Dalhousie Distributed Research Institute at Dalhousie University, the Western Canada Research Grid, and the Centre for Computer Assisted Research Mathematics and its Applications, University of Newcastle. The workshops that were held over the years in Dr. Borwein’s honor attracted high-caliber scientists from a wide range of mathematical fields. This present volume is an outgrowth of the workshop on ‘Computational and Analytical Mathematics’ held in May 2011 in celebration of Dr. Borwein’s 60th Birthday. The collection contains various state-of-the-art research manuscripts and surveys presenting contributions that have risen from the conference, and is an excellent opportunity to survey state-of-the-art research and discuss promising research directions and approaches.
Book Synopsis Correlative Light and Electron MIcroscopy by : Thomas Muller-Reichert
Download or read book Correlative Light and Electron MIcroscopy written by Thomas Muller-Reichert and published by Academic Press. This book was released on 2012-08-10 with total page 466 pages. Available in PDF, EPUB and Kindle. Book excerpt: The combination of electron microscopy with transmitted light microscopy (termed correlative light and electron microscopy; CLEM) has been employed for decades to generate molecular identification that can be visualized by a dark, electron-dense precipitate. This new volume of Methods in Cell Biology covers many areas of CLEM, including a brief history and overview on CLEM methods, imaging of intermediate stages of meiotic spindle assembly in C. elegans embryos using CLEM, and capturing endocytic segregation events with HPF-CLEM. Covers many areas of CLEM by the best international scientists in the field Includes a brief history and overview on CLEM methods
Download or read book Dendrites written by Kazuo Emoto and published by Springer. This book was released on 2016-08-19 with total page 596 pages. Available in PDF, EPUB and Kindle. Book excerpt: Studies in human patients and animal models of disease suggest a strong correlation between defects in dendrite development and common neurological disorders such as autism. Much of this book is thus dedicated toward highlighting recent advances in our understanding of the cellular and molecular mechanisms that regulate the development and maintenance of dendrites, a crucial component of neurons. The book begins by presenting the current state of knowledge on the building blocks or cell biology of dendrites. Mechanisms that sculpt the stereotypic architecture of dendritic arbors and shape their connectivity are also discussed, along with recent work describing how dendritic organization and connectivity are perturbed in disease. A unique aspect of the book is its exploration of diverse neuronal cell types across vertebrates and invertebrates, allowing a comparison of mechanisms across distinct circuits and species. The book comprises six parts, which cover the major advances in the field: Part 1, Introduction; Part 2, Basic Biology of Dendrites; Part 3, Patterning Dendritic Architecture of Neurons and Their Populations; Part 4, Cellular and Molecular Control of Dendrite Development and Maintenance; Part 5, Synapse Formation onto Dendrites; and Part 6, Dendrites in Disease. The book offers an excellent point of entry for students interested in neuroscience, as well as for clinicians.
Book Synopsis Alterations to Synaptic Function and Connectivity in Area CA3 of the Hippocampus in Mouse Models of Mental Retardation by : Ricardo Andres Valenzuela
Download or read book Alterations to Synaptic Function and Connectivity in Area CA3 of the Hippocampus in Mouse Models of Mental Retardation written by Ricardo Andres Valenzuela and published by Stanford University. This book was released on 2011 with total page 165 pages. Available in PDF, EPUB and Kindle. Book excerpt: Down Syndrome and Fragile X Syndrome are disorders of mental retardation that are characterized by cognitive impairments and changes to other physical characteristics. A goal in the study of these diseases has been to understand the mechanisms that underlie the cognitive impairments present in these two disorders of mental retardation. A great deal of effort has been made to study synaptic function and structure in these disorders of mental retardation in order to determine whether there are any alterations present. Alterations to synaptic structure and function present during these disorders may give insight to the neural basis of the cognitive impairments that are characteristic of this disorder of mental retardation. An area of the brain that may be affected by these disorders is the hippocampus. This area of the brain has been extensively studied for its role in memory and alterations to synaptic function and structure may underlie some of the memory deficits present in these disorders of mental retardation. Both Down Syndrome patients and Fragile X Syndrome patients have deficits in their performance on memory tests. Down Syndrome patients also have a reduction in the number of neurons present in the hippocampus (Carlesimo et al., 1997). Fragile X Syndrome patients had structural abnormalities in the hippocampus including an enlargement of ventricular spaces (Jakala et al., 1997). Synaptic function and structure in the hippocampus of mouse models of Down Syndrome and Fragile X Syndrome were studied in order to determine this region of the brain was affected. Electrophysiology recordings in area CA3 of the hippocampus of the Ts65Dn Down Syndrome mouse model indicated there were disruptions to synaptic connectivity, decreases in excitatory and inhibitory synaptic transmission, and also a reduction in intrinsic interneuron activity. Imaging studies of CA3 in the Ts65Dn mouse did not show alterations to the number of synapses or structure of synapses suggesting that the alterations found with electrophysiology recordings are the result of functional changes to synapses. Electrophysiology study of the hippocampus in mouse models of Fragile X Syndrome has shown that inhibitory function was generally intact but that excitatory axons from neurons that lacked the Fragile X Mental Retardation Protein (FMRP) were less competitive at forming synapses in a mosaic expression system of the Fmr1 gene the lack of which causes the disease. These studies indicate that alterations to synaptic structure and function are present in the hippocampus of these mouse models of mental retardation. The differences however, were not the same in Down Syndrome and Fragile X Syndrome mouse models. Nonetheless, it is possible that the changes to synaptic function found in both of these mouse models leads to altered network function in the hippocampus which may, in turn, be the underlying cause of the memory deficits present in these disorders of mental retardation. The data presented in these studies indicate that the study of these mouse models of mental retardation can give insight to alterations caused by these disorders of mental retardation which may also lead to the development of new treatments.
