Phage Display

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Phage Display

Author : Carlos F. Barbas
Publisher : CSHL Press
ISBN 13 : 0879697407
Total Pages : 724 pages
Book Rating : 4.8/5 (796 download)

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Book Synopsis Phage Display by : Carlos F. Barbas

Download or read book Phage Display written by Carlos F. Barbas and published by CSHL Press. This book was released on 2001 with total page 724 pages. Available in PDF, EPUB and Kindle. Book excerpt: Phage-display technology has begun to make critical contributions to the study of molecular recognition. DNA sequences are cloned into phage, which then present on their surface the proteins encoded by the DNA. Individual phage are rescued through interaction of the displayed protein with a ligand, and the specific phage is amplified by infection of bacteria. Phage-display technology is powerful but challenging and the aim of this manual is to provide comprehensive instruction in its theoretical and applied so that any scientist with even modest molecular biology experience can effectively employ it. The manual reflects nearly a decade of experience with students of greatly varying technical expertise andexperience who attended a course on the technology at Cold Spring Harbor Laboratory. Phage-display technology is growing in importance and power. This manual is an unrivalled source of expertise in its execution and application.

Phage Display

Author : Tim Clackson
Publisher : OUP Oxford
ISBN 13 : 0191516457
Total Pages : 358 pages
Book Rating : 4.1/5 (915 download)

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Book Synopsis Phage Display by : Tim Clackson

Download or read book Phage Display written by Tim Clackson and published by OUP Oxford. This book was released on 2004-03-04 with total page 358 pages. Available in PDF, EPUB and Kindle. Book excerpt: This new book is designed to enable researchers to design and undertake all aspects of a phage display project, from designing an experimental strategy and constructing a library to performing selections and analyzing the results.All of the protocols and chapters are extensively cross-referenced, allowing readers to move beyond the specific examples provided in order to customize the procedures for their own protein or selection system of interest. Phage Display is an up-to-date, comprehensive and integrated experimental guide to the technique, which is essential reading for anyone currently using, or wishing to use the technique for basic research and drug discovery.

Phage Display of Peptides and Proteins

Author : Brian K. Kay
Publisher : Elsevier
ISBN 13 : 0080538703
Total Pages : 369 pages
Book Rating : 4.0/5 (85 download)

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Book Synopsis Phage Display of Peptides and Proteins by : Brian K. Kay

Download or read book Phage Display of Peptides and Proteins written by Brian K. Kay and published by Elsevier. This book was released on 1996-10-23 with total page 369 pages. Available in PDF, EPUB and Kindle. Book excerpt: Both novices and experts will benefit from this insightful step-by-step discussion of phage display protocols.Phage Display of Peptides and Proteins: A Laboratory Manual reviews the literature and outlines the strategies for maximizing the successful application of phage display technology to one's research. It contains the most up-to-date protocols for preparing peptide affinity reagents, monclonal antibodies, and evolved proteins. - Prepared by experts in the field - Provides proven laboratory protocols, troubleshooting, and tips - Includes maps, sequences, and sample data - Contains extensive and up-to-date references

Antibody Phage Display

Author : Robert Aitken
Publisher : Methods in Molecular Biology
ISBN 13 :
Total Pages : 260 pages
Book Rating : 4.3/5 (91 download)

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Book Synopsis Antibody Phage Display by : Robert Aitken

Download or read book Antibody Phage Display written by Robert Aitken and published by Methods in Molecular Biology. This book was released on 2009-07-16 with total page 260 pages. Available in PDF, EPUB and Kindle. Book excerpt: In Antibody Phage Display expert researchers explore the latest in this cutting-edge technology, providing an invaluable resource that will guide readers in the design and execution of experiments based around antibody phage display.

Phage Display In Biotechnology and Drug Discovery

Author : Sachdev S. Sidhu
Publisher : CRC Press
ISBN 13 : 0849359120
Total Pages : 770 pages
Book Rating : 4.8/5 (493 download)

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Book Synopsis Phage Display In Biotechnology and Drug Discovery by : Sachdev S. Sidhu

Download or read book Phage Display In Biotechnology and Drug Discovery written by Sachdev S. Sidhu and published by CRC Press. This book was released on 2005-07-27 with total page 770 pages. Available in PDF, EPUB and Kindle. Book excerpt: The first and only guide to showcase the impact of phage display technology on drug discovery, this reference details the theories, principles, and methods impacting the field and demonstrates applications for peptide phage display, protein phage display, and the development of novel antibodies. Highlighting the current and future role of phage dis

Harnessing the Power of Viruses

Author : Boriana Marintcheva
Publisher : Academic Press
ISBN 13 : 0128105151
Total Pages : 298 pages
Book Rating : 4.1/5 (281 download)

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Book Synopsis Harnessing the Power of Viruses by : Boriana Marintcheva

Download or read book Harnessing the Power of Viruses written by Boriana Marintcheva and published by Academic Press. This book was released on 2017-11-13 with total page 298 pages. Available in PDF, EPUB and Kindle. Book excerpt: Harnessing the Power of Viruses explores the application of scientific knowledge about viruses and their lives to solve practical challenges and further advance molecular sciences, medicine and agriculture. The book contains virus-based tools and approaches in the fields of: i) DNA manipulations in vitro and in vivo; ii) Protein expression and characterization; and iii) Virus- Host interactions as a platform for therapy and biocontrol are discussed. It steers away from traditional views of viruses and technology, focusing instead on viral molecules and molecular processes that enable science to better understand life and offer means for addressing complex biological phenomena that positively influence everyday life. The book is written at an intermediate level and is accessible to novices who are willing to acquire a basic level of understanding of key principles in molecular biology, but is also ideal for advanced readers interested in expanding their biological knowledge to include practical applications of molecular tools derived from viruses. - Explores virus-based tools and approaches in DNA manipulation, protein expression and characterization and virus-host interactions - Provides a dedicated focus on viral molecules and molecular processes that enable science to better understand life and address complex biological phenomena - Includes an overview of modern technologies in biology that were developed using viral components/elements and knowledge about viral processes

Antibody Phage Display

Author : Philippa M. O’Brien
Publisher : Springer Science & Business Media
ISBN 13 : 1592592406
Total Pages : 391 pages
Book Rating : 4.5/5 (925 download)

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Book Synopsis Antibody Phage Display by : Philippa M. O’Brien

Download or read book Antibody Phage Display written by Philippa M. O’Brien and published by Springer Science & Business Media. This book was released on 2008-02-05 with total page 391 pages. Available in PDF, EPUB and Kindle. Book excerpt: The closing years of the 19th century and the start of the 20th century witnessed the emergence of microbiology and immunology as discrete sci- tific disciplines, and in the work of Roux and Yersin, perhaps the first benefits of their synergy—immunotherapy against bacterial infection. As we advance into the new millennium, microbiology and immunology again offer a c- ceptual leap forward as antibody phage display gains increasing acceptance as the definitive technology for monoclonal production and unleashes new - portunities in immunotherapy, drug discovery, and functional genomics. In assembling Antibody Phage Display: Methods and Protocols, we have aimed to produce a resource of real value for scientists who have followed the development of phage display technology over the past decade. The founding principles of phage display have always held an elegant simplicity. We hope that readers will find similar clarity in the technical guidance offered by the book’s contributors. In meeting our objectives, we have tried to cover the broad scope of the technology and the key areas of library construction, scre- ing, antibody modification, and expression. Of course, the technology cont- ues to advance apace, but we trust that readers will be able to gage the potential of phage display from our coverage, that some of its subtleties will emerge, and that our selection of methods will prove appealing. We are indebted to all the contributing authors for sharing their expertise with the wider scientific community.

Antibody Drug Discovery

Author : Clive R. Wood
Publisher : World Scientific
ISBN 13 : 1848166281
Total Pages : 490 pages
Book Rating : 4.8/5 (481 download)

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Book Synopsis Antibody Drug Discovery by : Clive R. Wood

Download or read book Antibody Drug Discovery written by Clive R. Wood and published by World Scientific. This book was released on 2012 with total page 490 pages. Available in PDF, EPUB and Kindle. Book excerpt: Antibody-based therapeutics are a central driver of the success of biopharmaceuticals. The discovery technology of this field is isolated to a limited number of centers of excellence in industry and academia. The objective of this volume is to provide a series of guides to those evaluating and preparing to enter particular areas within the field. Each chapter is written with a historical perspective that sets into context the significance of the key developments, and with the provision of “points to consider” for the reader as a value-added feature of the volume. All contributors are experts in their fields and have played pivotal roles in the creation of the technology.

Peptide, Protein and Enzyme Design

Author :
Publisher : Academic Press
ISBN 13 : 0128054344
Total Pages : 686 pages
Book Rating : 4.1/5 (28 download)

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Book Synopsis Peptide, Protein and Enzyme Design by :

Download or read book Peptide, Protein and Enzyme Design written by and published by Academic Press. This book was released on 2016-08-27 with total page 686 pages. Available in PDF, EPUB and Kindle. Book excerpt: De Novo Enzyme Design, the newest volume in the Methods in Enzymology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. This volume includes the design of metal binding maquettes, insertion of non-natural cofactors, Cu metallopeptides, non-covalent interactions in peptide assemblies, peptide binding and bundling, heteronuclear metalloenzymes, florinated peptides, De Novo imaging agents, and protein-protein interaction. - Continues the legacy of this premier serial with quality chapters on de novo enzyme design - Represents the newest volume in the Methods in Enzymology series, providing premier, quality chapters authored by leaders in the field - Ideal reference for those interested in the study of enzyme design that looks at both structure and mechanism

Antibody Engineering Volume 1

Author : Roland E. Kontermann
Publisher : Springer Science & Business Media
ISBN 13 : 3642011446
Total Pages : 770 pages
Book Rating : 4.6/5 (42 download)

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Book Synopsis Antibody Engineering Volume 1 by : Roland E. Kontermann

Download or read book Antibody Engineering Volume 1 written by Roland E. Kontermann and published by Springer Science & Business Media. This book was released on 2010-03-10 with total page 770 pages. Available in PDF, EPUB and Kindle. Book excerpt: Antibodies are indispensable tools for research, diagnosis, and therapy. Recombinant approaches allow the modification and improvement of nearly all antibody properties, such as affinity, valency, specificity, stability, serum half-life, effector functions, and immunogenicity. "Antibody Engineering" provides a comprehensive toolbox covering the well-established basics but also many exciting new techniques. The protocols reflect the latest "hands on" knowledge of key laboratories in this still fast-moving field. Newcomers will benefit from the proven step-by-step protocols, which include helpful practical advice; experienced antibody engineers will appreciate the new ideas and approaches. The book is an invaluable resource for all those engaged in antibody research and development.

Handbook of Immunoassay Technologies

Author : Sandeep K. Vashist
Publisher : Academic Press
ISBN 13 : 012811794X
Total Pages : 498 pages
Book Rating : 4.1/5 (281 download)

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Book Synopsis Handbook of Immunoassay Technologies by : Sandeep K. Vashist

Download or read book Handbook of Immunoassay Technologies written by Sandeep K. Vashist and published by Academic Press. This book was released on 2018-01-02 with total page 498 pages. Available in PDF, EPUB and Kindle. Book excerpt: Handbook of Immunoassay Technologies: Approaches, Performances, and Applications unravels the role of immunoassays in the biochemical sciences. During the last four decades, a wide range of immunoassays has been developed, ranging from the conventional enzyme-linked immunosorbent assays, to the smartphone-based point-of-care formats. The advances in rapid biochemical procedures, novel biosensing schemes, fully integrated lab-on-a-chip platforms, prolonged biomolecular storage strategies, device miniaturization and interfacing, and emerging smart system technologies equipped with personalized mobile healthcare tools are paving the way to next-generation immunoassays, and are all discussed in this comprehensive text. Immunoassays play a prominent role in clinical diagnostics as they are the eyes of healthcare professionals, helping them make informed clinical decisions via confirmed disease diagnosis, and thus enabling favorable health outcomes. The faster and reliable diagnosis of infections will further control their spread to uninfected persons. Similarly, immunoassays play a prominent role in veterinary diagnostics, food analysis, environmental monitoring, defense and security, and other bioanalytical settings. Therefore, they enable the detection of a plethora of analytes, which includes disease biomarkers, pathogens, drug impurities, environmental contaminants, allergens, food adulterants, drugs of abuse and various biomolecules. - Provides a valuable increase of understanding of cellular and biomedical functions - Gives the most updated resource in the field of immunoassays, providing the comprehensive details of various types of immunoassays that need to be performed in healthcare, and in industrial, environmental and other biochemical settings - Discusses all multifarious aspects of immunoassays - Describes the immunoassay formats, along with their principle of operation, characteristics, pros and cons, and potential biochemical and bioanalytical applications - Provides extensive knowledge and guided insights as detailed by experienced, renowned experts and key opinion makers in the field of immunoassays

Bacteriophages in Health and Disease

Author : Paul Hyman
Publisher : CABI
ISBN 13 : 1845939840
Total Pages : 296 pages
Book Rating : 4.8/5 (459 download)

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Book Synopsis Bacteriophages in Health and Disease by : Paul Hyman

Download or read book Bacteriophages in Health and Disease written by Paul Hyman and published by CABI. This book was released on 2012 with total page 296 pages. Available in PDF, EPUB and Kindle. Book excerpt: Bacteriophages are viruses that infect bacteria; as such, they have many potential uses for promoting health and combating disease. This book covers the many facets of phage-bacterial-human interaction in three sections: the role and impact of phages on natural bacterial communities, the potential to develop phage-based therapeutics and other aspects in which phages can be used to combat disease, including bacterial detection, bacterial epidemiology, the tracing of fecal contamination of water and decontamination of foods.

Mims' Medical Microbiology and immunology

Author : Richard Goering
Publisher : Elsevier Health Sciences
ISBN 13 : 0702072028
Total Pages : 662 pages
Book Rating : 4.7/5 (2 download)

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Book Synopsis Mims' Medical Microbiology and immunology by : Richard Goering

Download or read book Mims' Medical Microbiology and immunology written by Richard Goering and published by Elsevier Health Sciences. This book was released on 2018-02-27 with total page 662 pages. Available in PDF, EPUB and Kindle. Book excerpt: Learn all the microbiology and basic immunology concepts you need to know for your courses and exams. Now fully revised and updated, Mims' clinically relevant, systems-based approach and abundant colour illustrations make this complex subject easy to understand and remember. - Learn about infections in the context of major body systems and understand why these are environments in which microbes can establish themselves, flourish, and give rise to pathologic changes. This systems-based approach to microbiology employs integrated and case-based teaching that places the 'bug parade' into a clinical context. - Effectively review for problem-based courses with the help of chapter introductions and 'Lessons in Microbiology' text boxes that highlight the clinical relevance of the material, offer easy access to key concepts, and provide valuable review tools. - Approach microbiology by body system or by pathogen through the accompanying electronic 'Pathogen Parade' – a quickly searchable, cross-referenced glossary of viruses, bacteria and fungi - A new electronic 'Vaccine Parade' offers quick-reference coverage of the most commonly used vaccines in current clinical practice - Deepen your understanding of epidemiology and the important role it plays in providing evidence-based identification of key risk factors for disease and targets for preventative medicine. - Grasp and retain vital concepts easily, with a user-friendly colour coded format, succinct text, key concept boxes, and dynamic illustrations. - New and enhanced information reflects the growing importance of the human microbiota and latest molecular approaches - Access the complete contents on the go via the accompanying interactive eBook, with a range of bonus materials to enhance learning and retention – includes self-assessment materials and clinical cases to check your understanding and aid exam preparation.

Peptide Macrocycles

Author : Matthew B. Coppock
Publisher : Humana
ISBN 13 : 9781071616888
Total Pages : 469 pages
Book Rating : 4.6/5 (168 download)

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Book Synopsis Peptide Macrocycles by : Matthew B. Coppock

Download or read book Peptide Macrocycles written by Matthew B. Coppock and published by Humana. This book was released on 2021-11-02 with total page 469 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume explores the latest techniques and strategies used to study the field of peptide macrocycles. The chapters in this book ae organized into four parts: macrocycles synthesis, combinational library synthesis and screening, macrocycle characterization, and unique applications. Part One looks at a variety of peptide cyclization methodologies, and Part Two describes methods for the creation of peptide macrocycles libraries and their subsequent screening against biological targets of interest. Part Three discusses the study and characterization of peptide macrocycle-target interactions, and Part Four introduces unique applications for peptide macrocycles, from higher-order structure formation to post-synthetic functional modifications. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, Peptide Macrocycles: Methods and Protocols is a valuable resource for both novice and expert researchers looking to learn more about this developing field.

Membrane Proteins – Engineering, Purification and Crystallization

Author :
Publisher : Academic Press
ISBN 13 : 0128021934
Total Pages : 663 pages
Book Rating : 4.1/5 (28 download)

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Book Synopsis Membrane Proteins – Engineering, Purification and Crystallization by :

Download or read book Membrane Proteins – Engineering, Purification and Crystallization written by and published by Academic Press. This book was released on 2015-04-30 with total page 663 pages. Available in PDF, EPUB and Kindle. Book excerpt: Membrane Proteins – Engineering, Purification and Crystallization, a volume of Methods In Enzymology, encompasses chapters from the leading experts in the area of membrane protein biology. The chapters provide a brief overview of the topics covered and also outline step-by-step protocol for the interested audience. Illustrations and case example images are included wherever appropriate to help the readers understand the schematics and general experimental outlines. - Volume of Methods In Enzymology - Contains a collection of a diverse array of topics in the area of membrane protein biology ranging from recombinant expression, isolation, functional characterization, biophysical studies and crystallization

Human Monoclonal Antibodies

Author : Michael Steinitz
Publisher : Humana Press
ISBN 13 : 9781627035859
Total Pages : 0 pages
Book Rating : 4.0/5 (358 download)

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Book Synopsis Human Monoclonal Antibodies by : Michael Steinitz

Download or read book Human Monoclonal Antibodies written by Michael Steinitz and published by Humana Press. This book was released on 2013-09-14 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: The introduction of monoclonal antibodies revolutionized immunology. The development of human monoclonal antibodies was inspired primarily by the enormous clinical benefits promised by these reagents which can be used as anti-inflammatory reagents, anti-tumor reagents and reagents for passive immunization in a variety of pathologies. Human Monoclonal Antibodies: Methods and Protocols presents technical protocols of cellular and molecular methods for the production, purification and application of human monoclonal antibodies, as well as review articles on related topics of human monoclonal and polyclonal antibodies. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, Human Monoclonal Antibodies: Methods and Protocols seeks to serve both professionals and novices with its well-honed methodologies which will prove invaluable in a clinical setting.

Protein Engineering

Author : Huimin Zhao
Publisher : John Wiley & Sons
ISBN 13 : 3527344705
Total Pages : 41 pages
Book Rating : 4.5/5 (273 download)

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Book Synopsis Protein Engineering by : Huimin Zhao

Download or read book Protein Engineering written by Huimin Zhao and published by John Wiley & Sons. This book was released on 2021-08-23 with total page 41 pages. Available in PDF, EPUB and Kindle. Book excerpt: A one-stop reference that reviews protein design strategies to applications in industrial and medical biotechnology Protein Engineering: Tools and Applications is a comprehensive resource that offers a systematic and comprehensive review of the most recent advances in the field, and contains detailed information on the methodologies and strategies behind these approaches. The authors—noted experts on the topic—explore the distinctive advantages and disadvantages of the presented methodologies and strategies in a targeted and focused manner that allows for the adaptation and implementation of the strategies for new applications. The book contains information on the directed evolution, rational design, and semi-rational design of proteins and offers a review of the most recent applications in industrial and medical biotechnology. This important book: Covers technologies and methodologies used in protein engineering Includes the strategies behind the approaches, designed to help with the adaptation and implementation of these strategies for new applications Offers a comprehensive and thorough treatment of protein engineering from primary strategies to applications in industrial and medical biotechnology Presents cutting edge advances in the continuously evolving field of protein engineering Written for students and professionals of bioengineering, biotechnology, biochemistry, Protein Engineering: Tools and Applications offers an essential resource to the design strategies in protein engineering and reviews recent applications.