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Pcr Primer Design
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Book Synopsis PCR Primer Design by : Chhandak Basu
Download or read book PCR Primer Design written by Chhandak Basu and published by . This book was released on 2021 with total page 276 pages. Available in PDF, EPUB and Kindle. Book excerpt: This third edition provides new and updated chapters on design PCR primers for successful DNA amplification. Chapters are divided into seven parts, including primer design strategies for quantitative PCR, genotyping, multiplex PCR, in silico PCR primer design, and primer design to identify plant and animal viruses. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, PCR Primer Design, Third Edition aims to be useful for various fields of molecular biology, including biotechnology, molecular genetics, and recombinant DNA technology.
Book Synopsis PCR Primer Design by : Chhandak Basu
Download or read book PCR Primer Design written by Chhandak Basu and published by Humana. This book was released on 2015-02-19 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume provides an overview on design PCR primers for successful DNA amplification. Chapters focus on primer design strategies for quantitative PCR, in silico PCR primer design, and primer design using software. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, PCR Primer Design, Second Edition seeks to aid molecular biology students, researchers, professors and PCR enthusiasts.
Book Synopsis Basic Applied Bioinformatics by : Chandra Sekhar Mukhopadhyay
Download or read book Basic Applied Bioinformatics written by Chandra Sekhar Mukhopadhyay and published by John Wiley & Sons. This book was released on 2017-09-15 with total page 530 pages. Available in PDF, EPUB and Kindle. Book excerpt: An accessible guide that introduces students in all areas of life sciences to bioinformatics Basic Applied Bioinformatics provides a practical guidance in bioinformatics and helps students to optimize parameters for data analysis and then to draw accurate conclusions from the results. In addition to parameter optimization, the text will also familiarize students with relevant terminology. Basic Applied Bioinformatics is written as an accessible guide for graduate students studying bioinformatics, biotechnology, and other related sub-disciplines of the life sciences. This accessible text outlines the basics of bioinformatics, including pertinent information such as downloading molecular sequences (nucleotide and protein) from databases; BLAST analyses; primer designing and its quality checking, multiple sequence alignment (global and local using freely available software); phylogenetic tree construction (using UPGMA, NJ, MP, ME, FM algorithm and MEGA7 suite), prediction of protein structures and genome annotation, RNASeq data analyses and identification of differentially expressed genes and similar advanced bioinformatics analyses. The authors Chandra Sekhar Mukhopadhyay, Ratan Kumar Choudhary, and Mir Asif Iquebal are noted experts in the field and have come together to provide an updated information on bioinformatics. Salient features of this book includes: Accessible and updated information on bioinformatics tools A practical step-by-step approach to molecular-data analyses Information pertinent to study a variety of disciplines including biotechnology, zoology, bioinformatics and other related fields Worked examples, glossary terms, problems and solutions Basic Applied Bioinformatics gives students studying bioinformatics, agricultural biotechnology, animal biotechnology, medical biotechnology, microbial biotechnology, and zoology an updated introduction to the growing field of bioinformatics.
Download or read book PCR Primer written by Carl W. Dieffenbach and published by CSHL Press. This book was released on 2003 with total page 544 pages. Available in PDF, EPUB and Kindle. Book excerpt: The Polymerase Chain Reaction (PCR) technique was invented nearly 20 years ago. Its subsequent variations and applications were many and varied, and today molecular biology, clinical, and forensic laboratories make almost daily use of PCR. This second edition of the much-praised PCR Primer: A Laboratory Manual updates the tried-and-true methods and presents the advances made in the 10 years since the first edition. After introducing the basics for PCR and methods of sample preparation, PCR Primer provides laboratory-tested protocols for RT-PCR methods, detection of PCR products, analysis of differential expression, cloning, and mutagenesis. These step-by-step methods include extensive background information, as well as valuable troubleshooting information provided by the leading experts in this technology. This manual is a comprehensive and reliable source of the full range of PCR methods for novices and experienced investigators alike.
Download or read book PCR Primer Design written by Anton Yuryev and published by Springer Science & Business Media. This book was released on 2008-02-03 with total page 870 pages. Available in PDF, EPUB and Kindle. Book excerpt: At the heart of most high-throughput methods is the technique of polymerase chain reaction (PCR). This book focuses on primer design, which is critical to both the efficiency and the accuracy of the PCR. With intricate descriptions of basic approaches as well as specialized methods, "PCR Primer Design" is an exceptional reference for all those involved in studying the genome.
Book Synopsis The Soybean Genome by : Henry T. Nguyen
Download or read book The Soybean Genome written by Henry T. Nguyen and published by Springer. This book was released on 2017-09-20 with total page 211 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book examines the application of soybean genome sequences to comparative, structural, and functional genomics. Since the availability of the soybean genome sequence has revolutionized molecular research on this important crop species, the book also describes how the genome sequence has shaped research on transposon biology and applications for gene identification, tilling and positional gene cloning. Further, the book shows how the genome sequence influences research in the areas of genetic mapping, marker development, and genome-wide association mapping for identifying important trait genes and soybean breeding. In closing, the economic and botanical aspects of the soybean are also addressed.
Download or read book RT-PCR Protocols written by Nicola King and published by Springer Science & Business Media. This book was released on 2008-02-04 with total page 370 pages. Available in PDF, EPUB and Kindle. Book excerpt: Until the mid 1980s, the detection and quantification of a specific mRNA was a difficult task, usually only undertaken by a skilled molecular biologist. With the advent of PCR, it became possible to amplify specific mRNA, after first converting the mRNA to cDNA via reverse transcriptase. The arrival of this technique—termed reverse transcription-PCR (RT-PCR)—meant that mRNA suddenly became amenable to rapid and sensitive analysis, without the need for advanced training in molecular biology. This new accessibility of mRNA, which has been facilitated by the rapid accumulation of sequence data for human mRNAs, means that every biomedical researcher can now include measurement of specific mRNA expression as a routine component of his/her research plans. In view of the ubiquity of the use of standard RT-PCR, the main objective of RT-PCR Protocols is essentially to provide novel, useful applications of RT-PCR. These include some useful adaptations and applications that could be relevant to the wider research community who are already familiar with the basic RT-PCR protocol. For example, a variety of different adaptations are described that have been employed to obtain quantitative data from RT-PCR. Quantitative RT-PCR provides the ability to accurately measure changes/imb- ances in specific mRNA expression between normal and diseased tissues.
Book Synopsis PCR Protocols by : John M. S. Bartlett
Download or read book PCR Protocols written by John M. S. Bartlett and published by Springer Science & Business Media. This book was released on 2008-02-03 with total page 1083 pages. Available in PDF, EPUB and Kindle. Book excerpt: In this new edition, the editors have thoroughly updated and dramatically expanded the number of protocols to take advantage of the newest technologies used in all branches of research and clinical medicine today. These proven methods include real time PCR, SNP analysis, nested PCR, direct PCR, and long range PCR. Among the highlights are chapters on genome profiling by SAGE, differential display and chip technologies, the amplification of whole genome DNA by random degenerate oligonucleotide PCR, and the refinement of PCR methods for the analysis of fragmented DNA from fixed tissues. Each fully tested protocol is described in step-by-step detail by an established expert in the field and includes a background introduction outlining the principle behind the technique, equipment and reagent lists, tips on trouble shooting and avoiding known pitfalls, and, where needed, a discussion of the interpretation and use of results.
Author :Elizabeth van Pelt-Verkuil Publisher :Springer Science & Business Media ISBN 13 :1402062419 Total Pages :333 pages Book Rating :4.4/5 (2 download)
Book Synopsis Principles and Technical Aspects of PCR Amplification by : Elizabeth van Pelt-Verkuil
Download or read book Principles and Technical Aspects of PCR Amplification written by Elizabeth van Pelt-Verkuil and published by Springer Science & Business Media. This book was released on 2008-03-14 with total page 333 pages. Available in PDF, EPUB and Kindle. Book excerpt: Kary Mullis was awarded a Nobel Prize for inventing the PCR technique more than a decade ago in 1993. Since its "discovery", multiple adaptations and variations of the standard PCR technique have been described. This publication aims to provide the reader with a guide to the standard PCR technique and its many available variants, with particular emphasis being placed on the role of these PCR techniques in the clinical diagnostic laboratory (the central theme of this book).
Book Synopsis Quantitative Real-Time PCR by : Roberto Biassoni
Download or read book Quantitative Real-Time PCR written by Roberto Biassoni and published by Humana. This book was released on 2014-04-17 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Quantitative Real-Time PCR: Methods and Protocols focuses on different applications of qPCR ranging from microbiological detections (both viral and bacterial) to pathological applications. Several chapters deal with quality issues which regard the quality of starting material, the knowledge of the minimal information required to both perform an assay and to set the experimental plan, while the others focus on translational medicine applications that are ordered following an approximate logical order of their medical application. The last part of the book gives you an idea of an emerging digital PCR technique that is a unique qPCR approach for measuring nucleic acid, particularly suited for low level detection and to develop non-invasive diagnosis. Written for the Methods in Molecular Biology series, most chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, laboratory protocols and tips on troubleshooting and avoiding known pitfalls. Practical and authoritative, Quantitative Real-Time PCR: Methods and Protocols aims to aid researchers seeking to devise new qPCR-based approaches related to his or her area of investigation.
Book Synopsis Molecular Diagnostics of Infectious Diseases by : Harald H. Kessler
Download or read book Molecular Diagnostics of Infectious Diseases written by Harald H. Kessler and published by Walter de Gruyter GmbH & Co KG. This book was released on 2014-07-28 with total page 243 pages. Available in PDF, EPUB and Kindle. Book excerpt: The prevalence of infectious diseases is worldwide increasing. Therefore, detection methods for infectious pathogens change quickly. In the 3rd edition of Kessler ́s Molecular Diagnostics of Infectious Diseases laboratory professionalists get valueable information about the current diagnostic methods, tipps and tricks in terms of sample processing, quality control, and interpretation of the results. For clinicians the book is a valuable aid for decision-making in ordering appropriate tests as well as in assuring the necessary quality of the sample material.
Book Synopsis Laboratory Methods in Enzymology: DNA by :
Download or read book Laboratory Methods in Enzymology: DNA written by and published by Elsevier. This book was released on 2013-09-02 with total page 405 pages. Available in PDF, EPUB and Kindle. Book excerpt: Methods in Enzymology volumes provide an indispensable tool for the researcher. Each volume is carefully written and edited by experts to contain state-of-the-art reviews and step-by-step protocols. In this volume, we have brought together a number of core protocols concentrating on DNA, complementing the traditional content that is found in past, present and future Methods in Enzymology volumes. Indispensable tool for the researcher Carefully written and edited by experts to contain step-by-step protocols In this volume we have brought together a number of core protocols concentrating on DNA
Book Synopsis Bioinformatics Research and Development by : Sepp Hochreiter
Download or read book Bioinformatics Research and Development written by Sepp Hochreiter and published by Springer. This book was released on 2007-05-21 with total page 482 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book constitutes the refereed proceedings of the First International Bioinformatics Research and Development Conference, BIRD 2007, held in Berlin, Germany in March 2007. The 36 revised full papers are organized in topical sections on microarray and systems biology and networks, medical, SNPs, genomics, systems biology, sequence analysis and coding, proteomics and structure, databases, Web and text analysis.
Book Synopsis Forensic DNA Profiling Protocols by : Patrick J. Lincoln
Download or read book Forensic DNA Profiling Protocols written by Patrick J. Lincoln and published by Springer Science & Business Media. This book was released on 1998-01-22 with total page 617 pages. Available in PDF, EPUB and Kindle. Book excerpt: This state-of-the-art collection of easily reproducible methods includes all of the major techniques of DNA analysis currently used in forensic identity testing. The methods include the recovery of DNA from a large range of sample types, analysis of DNA as single and multi-locus VNTR probes, PCR amplification of STR and other loci, and mitochondrial sequencing. The expert scientists writing here -- many from laboratories around the world -- also discuss how to interpret the results in cases of unknown identity and disputed parentage.-- Covers all steps from extraction of human DNA through to analysis and interpretation-- Takes advantage of new methodologies such as capillary electrophoresis-- Clear step-by-step instructions ensure unfailing reproducibility.
Book Synopsis Biochemistry Laboratory Manual For Undergraduates by : Timea Gerczei Fernandez
Download or read book Biochemistry Laboratory Manual For Undergraduates written by Timea Gerczei Fernandez and published by Walter de Gruyter GmbH & Co KG. This book was released on 2015-03-11 with total page 208 pages. Available in PDF, EPUB and Kindle. Book excerpt: Biochemistry laboratory manual for undergraduates – an inquiry based approach by Gerczei and Pattison is the first textbook on the market that uses a highly relevant model, antibiotic resistance, to teach seminal topics of biochemistry and molecular biology while incorporating the blossoming field of bioinformatics. The novelty of this manual is the incorporation of a student-driven real real-life research project into the undergraduate curriculum. Since students test their own mutant design, even the most experienced students remain engaged with the process, while the less experienced ones get their first taste of biochemistry research. Inclusion of a research project does not entail a limitation: this manual includes all classic biochemistry techniques such as HPLC or enzyme kinetics and is complete with numerous problem sets relating to each topic.
Download or read book PCR Protocols written by Michael A. Innis and published by Academic Press. This book was released on 2012-12-02 with total page 501 pages. Available in PDF, EPUB and Kindle. Book excerpt: The correct procedures you need for frustration-free PCR methods and applications are contained in this complete, step-by-step, clearly written, inexpensive manual. Avoid contamination--with specific instructions on setting up your lab Avoid cumbersome molecular biological techniques Discover new applications
Author :Army Center for MIlitary History Publisher :Government Printing Office ISBN 13 :9780160920936 Total Pages :132 pages Book Rating :4.9/5 (29 download)
Book Synopsis Vietnam Studies the War in the Northen Provinces 1966-1968 by : Army Center for MIlitary History
Download or read book Vietnam Studies the War in the Northen Provinces 1966-1968 written by Army Center for MIlitary History and published by Government Printing Office. This book was released on 2013-12-16 with total page 132 pages. Available in PDF, EPUB and Kindle. Book excerpt: An account of North Vietnamese attempts to seize control of Quang Tri and Thua Thien Provinces and the response of the allied forces, particularly U.S. Army units. Contents Chapter I. EARLY DEVELOPMENTS Background The Northern Border, 1965-1967 Continuing Activity Along the Demilitarized Zone II. PREPARING FOR A SHOWDOWN The Anti-Infiltration System Free World Forces The Growth of Logistic Facilities Upgrading of the Vietnamese Army Forces III. THE BLEAK PICTURE Operation Niagara. The Battle of Keh Sanh- Opening Round The Tet Offensive--First Phase The Battle for Hue Intelligence Battle for Quang Tri Enemy Attacks on the Logistical System Task Force Clearwater IV. U.S. RESPONSE TO THE TET OFFENSIVE Planning for the Reliel of Khe Sanh Single Manager for Air Concept V. KHE SANH AND PEGASUS Planning for Pegasus Operation Orders VI. THE FREE WORLD COUNTEROFFENSIVE Opening Operations Back to A Shau VII. ANALYSIS OF NORTH VIETNAMES GOALS AND FAILURES Intelligence Organization for Combat Airmobility Superior Firepower Communications Logistics Improvement of Vietnamese Armed Forces The Other War Conclusion GLOSSARY INDEX
