Read Books Online and Download eBooks, EPub, PDF, Mobi, Kindle, Text Full Free.
Download Pcr A Practical Approach 3 1998 Pcr In Situ Hybridization full books in PDF, epub, and Kindle. Read online Pcr A Practical Approach 3 1998 Pcr In Situ Hybridization ebook anywhere anytime directly on your device. Fast Download speed and no annoying ads. We cannot guarantee that every ebooks is available!
Author : C. S. Herrington
Publisher :
ISBN 13 :
Total Pages : 205 pages
Book Rating : 4.:/5 (778 download)
Download or read book PCR 3 written by C. S. Herrington and published by . This book was released on 1998 with total page 205 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author : C. S. Herrington
Publisher :
ISBN 13 :
Total Pages : 205 pages
Book Rating : 4.:/5 (112 download)
Download or read book PCR 3 written by C. S. Herrington and published by . This book was released on 1997 with total page 205 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author : C. Simon Herrington
Publisher : Oxford University Press
ISBN 13 : 0191565598
Total Pages : 223 pages
Book Rating : 4.1/5 (915 download)
Download or read book PCR 3 written by C. Simon Herrington and published by Oxford University Press. This book was released on 1997-10-30 with total page 223 pages. Available in PDF, EPUB and Kindle. Book excerpt: PCR in situ hybridization allows the detection of specific nucleic acid sequences and their distribution in the cell. It combines two powerful techniquesin situ hybridization (ISH), which allows cellular localization of DNA and RNA sequences in cells and tissues, and the polymerase chain reaction (PCR), which allows reproducible amplification of rare nucleic acid sequences. The combined technique and its variants greatly enhance the sensitivity of in situ hybridization and add morphological localization to the sensitivity of PCR. Such techniques have enormous potential for research and diagnosis but problems with reproducibility and reliability are often encountered. This book overcomes these problems by describing the key procedures in step-by-step detail and by providing the essential advice needed for success. Topics include: DNA in situ PCR and DNA PCR in situ hybridization (PCR ISH) for the detection of DNA targets in cells; reverse trancriptase in situ PCR (RT-PCR) and RT-PCR ISH for the detection of RNA targets; and PRINS (primed in situ synthesis) for chromosomal analysis in interphase nuclei and metaphase chromosome spreads. There are further chapters on fixation of tissues for PCR, selective ultraviolet radiation fractionation (SURF), application of in situ PCR to human tissues, applications and modifications of PCR-ISH, and automation of in situ amplification. PCR In Situ Hybridization is a unique and timely collection of well-tested protocols for the amplification of DNA and RNA in cells and tissues, drawing on the accumulated knowledge and experience of leading exponents of these techniques. For each topic covered, the authors provide detailed guidance on the key steps in the protocols, numerous hints and tips for success, and advice on trouble-shooting. PCR In Situ Hybridization will be invaluable to molecular biologists, pathologists, geneticists, and all those seeking to perform in situ analyses of nucleic acid molecules.
Author : C. S. Herrington
Publisher :
ISBN 13 : 9780199636334
Total Pages : 205 pages
Book Rating : 4.6/5 (363 download)
Download or read book PCR : a practical approach. 3 (1998). PCR in situ hybridization written by C. S. Herrington and published by . This book was released on 1998 with total page 205 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author : M. J. McPherson
Publisher : Oxford University Press, USA
ISBN 13 :
Total Pages : 300 pages
Book Rating : 4.:/5 (318 download)
Download or read book PCR written by M. J. McPherson and published by Oxford University Press, USA. This book was released on 1991 with total page 300 pages. Available in PDF, EPUB and Kindle. Book excerpt: Relatively simple to perform, yet extremely diverse in tis applications, polymerase chain reaction (PCR) has become an important tool in many areas of biomedical research. It enables scientists to produce large quantities of DNA from minute amounts of starting material. This book provides a general introduction to PCR for researchers new to the area. It covers gene analysis at the level of restriction enzyme polymorphism, point mutation and sequence analysis. Methods for using different source materials - cloned DNA, genomic DNA, RNA, nucleic acid from archive material and PCR products themselves - are given. The emphasis throughout is on the practical applications of the technique and detailed experimental protocols are included at all stages, as well as valuable advice on avoiding pitfalls and optimizing results. Although other manuals exist, this is the most up-to-date work of its kind and has the advantage of being presented in the popular Practical Approach format. It will be of value to researchers and advanced students working in a wide variety of fields, from molecular biology to medicine.
Author : M. J. McPherson
Publisher :
ISBN 13 : 0199634246
Total Pages : 362 pages
Book Rating : 4.1/5 (996 download)
Download or read book PCR 2 written by M. J. McPherson and published by . This book was released on 1995 with total page 362 pages. Available in PDF, EPUB and Kindle. Book excerpt: PCR (polymerase chain reaction) is now one of the most widely used of basic molecular biology techniques and is an indispensable research tool for the molecular biologist. The basic PCR technique provides the cornerstone for in vitro DNA amplification - allowing the researcher to producelarge quantities of DNA from minute amounts of starting material.PCR: A Practical Approach Volume 2 is not a revised version of PCR: A Pracical Approach, but sets out to address some of the exciting new applications fo PCR including cDNA cloning, mRNA quantitation and expression of proteins fromPCR products, genomic DNA mapping and fingerprinting, and mutational analysis.
Author :
Publisher :
ISBN 13 : 9780397587490
Total Pages : pages
Book Rating : 4.5/5 (874 download)
Download or read book PCR in Situ Hybridization written by and published by . This book was released on with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author : M. J. McPherson
Publisher :
ISBN 13 : 9780199631964
Total Pages : 0 pages
Book Rating : 4.6/5 (319 download)
Download or read book Polymerase Chain Reaction written by M. J. McPherson and published by . This book was released on 1991 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author : John M. S. Bartlett
Publisher : Springer Science & Business Media
ISBN 13 : 1592593844
Total Pages : 1083 pages
Book Rating : 4.5/5 (925 download)
Download or read book PCR Protocols written by John M. S. Bartlett and published by Springer Science & Business Media. This book was released on 2008-02-03 with total page 1083 pages. Available in PDF, EPUB and Kindle. Book excerpt: In this new edition, the editors have thoroughly updated and dramatically expanded the number of protocols to take advantage of the newest technologies used in all branches of research and clinical medicine today. These proven methods include real time PCR, SNP analysis, nested PCR, direct PCR, and long range PCR. Among the highlights are chapters on genome profiling by SAGE, differential display and chip technologies, the amplification of whole genome DNA by random degenerate oligonucleotide PCR, and the refinement of PCR methods for the analysis of fragmented DNA from fixed tissues. Each fully tested protocol is described in step-by-step detail by an established expert in the field and includes a background introduction outlining the principle behind the technique, equipment and reagent lists, tips on trouble shooting and avoiding known pitfalls, and, where needed, a discussion of the interpretation and use of results.
Download or read book PCR A Practical Approach, Vol. 1 ... written by and published by . This book was released on 1992 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author : Michael A. Innis
Publisher : Academic Press
ISBN 13 : 0080919634
Total Pages : 589 pages
Book Rating : 4.0/5 (89 download)
Download or read book PCR Applications written by Michael A. Innis and published by Academic Press. This book was released on 1999-05-11 with total page 589 pages. Available in PDF, EPUB and Kindle. Book excerpt: PCR is the most powerful technique currently used in molecular biology. It enables the scientist to quickly replicate DNA and RNA on the benchtop. From its discovery in the early 80's, PCR has blossomed into a method that enables everything from ready mutation of DNA/RNA to speedy analysis of tens of thousands of nucleotide sequences daily.PCR Applications examines the latest developments in this field. It is the third book in the series, building on the previous publications PCR Protocols and PCR Strategies. The manual discusses techniques that focus on gene discovery, genomics, and DNA array technology, which are contributing factors to the now-occurring bioinformatics boom.Key Features* Focuses on gene discovery, genomics, and DNA array technology* Covers quantitative PCR techniques, including the use of standards and kinetic analysisincludes statistical refinement of primer design parameters* Ilustrates techniques used in microscopic tissue samples, such as single cell PCR, whole cell PCR, laser capture microdissection, and in situ PCREntries provide information on:* Nomenclature* Expression* Sequence analysis* Structure and function* Electrophysiology* Parmacology* Information retrieval
Author : Julian E. Beesley
Publisher : Springer Science & Business Media
ISBN 13 : 146120139X
Total Pages : 277 pages
Book Rating : 4.4/5 (612 download)
Download or read book Immunocytochemistry and In Situ Hybridization in the Biomedical Sciences written by Julian E. Beesley and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 277 pages. Available in PDF, EPUB and Kindle. Book excerpt: Immunocytochemistry and in situ hybridization are widely used biomedical sciences. They are essential in medical diagnosis and in cell biology research. Affinity labeling is the central goal of the experimental strategy involving a series of techniques in a logical order; from the effects of specimen fixation, through specimen preparation to expose the antigen, to optimizing immunolabeling, to assessing the result and finally to safety considerations. Numerous examples of these techniques in biomedical sciences are included, as well as experimental assays and practical tips. This survey of methods will serve as an invaluable reference source in any laboratory setting (academic, industrial or clinical) involved in research in almost every branch of biology or medicine, as well as in pharmaceutical, biotechnological and clinical applications.
Author : Gerard J. Nuovo
Publisher : Lippincott Williams & Wilkins
ISBN 13 :
Total Pages : 536 pages
Book Rating : 4.3/5 (91 download)
Download or read book PCR in Situ Hybridization written by Gerard J. Nuovo and published by Lippincott Williams & Wilkins. This book was released on 1997 with total page 536 pages. Available in PDF, EPUB and Kindle. Book excerpt: Describes the technique whereby the extreme sensitivity of the polymerase chain reaction (PCR) is combined with the cell localizing ability of in situ hybridization. This revised and updated edition contains chapters on the basics of molecular biology; the nonspecific pathways of PCR; applications of PCR in situ hybridization--human papillomavirus, and HIV-1; and instrumentation. There is also an appendix on reagents for molecular biological analyses. Annotation copyright by Book News, Inc., Portland, OR
Author : Morteza Jalali
Publisher : Academic Press
ISBN 13 : 012803078X
Total Pages : 383 pages
Book Rating : 4.1/5 (28 download)
Download or read book Basic Science Methods for Clinical Researchers written by Morteza Jalali and published by Academic Press. This book was released on 2017-03-31 with total page 383 pages. Available in PDF, EPUB and Kindle. Book excerpt: Basic Science Methods for Clinical Researchers addresses the specific challenges faced by clinicians without a conventional science background. The aim of the book is to introduce the reader to core experimental methods commonly used to answer questions in basic science research and to outline their relative strengths and limitations in generating conclusive data. This book will be a vital companion for clinicians undertaking laboratory-based science. It will support clinicians in the pursuit of their academic interests and in making an original contribution to their chosen field. In doing so, it will facilitate the development of tomorrow's clinician scientists and future leaders in discovery science. - Serves as a helpful guide for clinical researchers who lack a conventional science background - Organized around research themes pertaining to key biological molecules, from genes, to proteins, cells, and model organisms - Features protocols, techniques for troubleshooting common problems, and an explanation of the advantages and limitations of a technique in generating conclusive data - Appendices provide resources for practical research methodology, including legal frameworks for using stem cells and animals in the laboratory, ethical considerations, and good laboratory practice (GLP)
Author : Wiley-VCH
Publisher : Wiley-VCH
ISBN 13 :
Total Pages : 488 pages
Book Rating : 4.:/5 (318 download)
Download or read book Ullmann's Biotechnology and Biochemical Engineering, 2 Volume Set written by Wiley-VCH and published by Wiley-VCH. This book was released on 2007-07-23 with total page 488 pages. Available in PDF, EPUB and Kindle. Book excerpt: The one-stop resource for all those involved in the biochemical and biotechnological industries. Based on the latest online edition of Ullmann's Encyclopedia of Industrial Chemistry containing articles never seen before in print, this ready reference meets the need for a detailed survey of the biochemical fundamentals and techniques as well as their applications in biochemical engineering and biobased production.
Author : R R Bowker Publishing
Publisher :
ISBN 13 : 9780835240871
Total Pages : 1312 pages
Book Rating : 4.2/5 (48 download)
Download or read book American Book Publishing Record Cumulative 1998 written by R R Bowker Publishing and published by . This book was released on 1999-03 with total page 1312 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author :
Publisher :
ISBN 13 :
Total Pages : 750 pages
Book Rating : 4.3/5 (91 download)
Download or read book Ullmann's Encyclopedia of Industrial Chemistry written by and published by . This book was released on 2003 with total page 750 pages. Available in PDF, EPUB and Kindle. Book excerpt:
